From owner-diagnost@hgmp.mrc.ac.uk Wed Nov 1 10:04:29 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 4FD2517B72; Wed, 1 Nov 2000 10:04:28 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id D395317B75; Wed, 1 Nov 2000 10:04:25 +0000 (GMT) To: diagnost@net.bio.net Newsgroups: bionet.diagnostics From: nobody@hgmp.mrc.ac.uk, [mailto:nobody@hgmp.mrc.ac.uk]@hgmp.mrc.ac.uk, On@hgmp.mrc.ac.uk, Behalf@hgmp.mrc.ac.uk, Of@hgmp.mrc.ac.uk Subject: How long can the coated ELISA plate be kept at 4 degree Message-Id: <20001101100425.D395317B75@mercury.hgmp.mrc.ac.uk> Date: Wed, 1 Nov 2000 10:04:25 +0000 (GMT) Sender: owner-diagnost@hgmp.mrc.ac.uk Precedence: bulk Hallo Dear Mr. I am measuring cytokines with ELISA now. I always coat the plate and keep it overnight at 4 degree. Sometime after I coated the plate, it happened that I couldn't block it and add samples on next day or even a few days. I don't know how long I can keep the coated plates at 4 degree. Would you please tell me about this question. Thanks in advance. Yous sincerely Meixia Zhang --- From owner-diagnost@hgmp.mrc.ac.uk Thu Nov 2 09:35:12 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 0396D17BBB; Thu, 2 Nov 2000 09:35:10 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 1D53A17BBE; Thu, 2 Nov 2000 09:35:08 +0000 (GMT) To: diagnost@net.bio.net Newsgroups: bionet.diagnostics From: news@hgmp.mrc.ac.uk, [mailto:news@theplanet.net]@hgmp.mrc.ac.uk, On@hgmp.mrc.ac.uk, Behalf@hgmp.mrc.ac.uk, Of@hgmp.mrc.ac.uk, David@hgmp.mrc.ac.uk, A@hgmp.mrc.ac.uk, C@hgmp.mrc.ac.uk, Jones@hgmp.mrc.ac.uk Subject: Re: How long can the coated ELISA plate be kept at 4 degree Message-Id: <20001102093508.1D53A17BBE@mercury.hgmp.mrc.ac.uk> Date: Thu, 2 Nov 2000 09:35:08 +0000 (GMT) Sender: owner-diagnost@hgmp.mrc.ac.uk Precedence: bulk Not a bad idea to do some comparisons, but when I was in the business I found that 3-4 weeks at 4oC seemed to show no degradation in quality. But different antigens/antibodies may behave differently Regards-- David A C Jones www.dacjones.co.uk Web Design Genealogy and Family Formerly a Scientist! wrote in message news:8topr7$c3s$1@mercury.hgmp.mrc.ac.uk... > > Hallo Dear Mr. > > I am measuring cytokines with ELISA now. I always coat the plate and > keep it overnight at 4 degree. Sometime after I coated the plate, it > happened that I couldn't block it and add samples on next day or even a > few days. I don't know how long I can keep the coated plates at 4 > degree. Would you please tell me about this question. > > > Thanks in advance. > > > Yous sincerely > > Meixia Zhang > > > --- > > From owner-diagnost@hgmp.mrc.ac.uk Mon Nov 6 11:51:17 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 79AAF17AFE; Mon, 6 Nov 2000 11:51:05 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id C983917B06; Mon, 6 Nov 2000 11:45:25 +0000 (GMT) To: diagnost@net.bio.net Newsgroups: bionet.diagnostics From: rout@demdwug2.mediaways.net, [mailto:rout@demdwug2.mediaways.net]@hgmp.mrc.ac.uk Subject: Re: Help Message-Id: <20001106114525.C983917B06@mercury.hgmp.mrc.ac.uk> Date: Mon, 6 Nov 2000 11:45:25 +0000 (GMT) Sender: owner-diagnost@hgmp.mrc.ac.uk Precedence: bulk nobody@hgmp.mrc.ac.uk, [mailto:nobody@hgmp.mrc.ac.uk], On, Behalf, Of schrieb: > Could anyone tell me where I can purchurse anti-human nestin primary > antibody ? > > thank you very much! hui59@263.net Try Biogenesis, Chemicon, BD Pharmingen. They all are referenced in Linscotts's. In Germany, ask DPC Biermann (+49 5221 349817) kind regards, RWM From owner-diagnost@hgmp.mrc.ac.uk Thu Nov 16 12:40:31 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id DAEBB17B05; Thu, 16 Nov 2000 12:40:29 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id AE8E417AF5; Thu, 16 Nov 2000 12:40:26 +0000 (GMT) To: diagnost@net.bio.net Newsgroups: bionet.diagnostics From: nobody@hgmp.mrc.ac.uk, [mailto:nobody@hgmp.mrc.ac.uk]@hgmp.mrc.ac.uk, On@hgmp.mrc.ac.uk, Behalf@hgmp.mrc.ac.uk, Of@hgmp.mrc.ac.uk Subject: identification of rDNA Message-Id: <20001116124026.AE8E417AF5@mercury.hgmp.mrc.ac.uk> Date: Thu, 16 Nov 2000 12:40:26 +0000 (GMT) Sender: owner-diagnost@hgmp.mrc.ac.uk Precedence: bulk Dear colleagues, I want to check plasmid inserts in a Southern HYB for clones with repetitive sequences. So I am planning to hybridyze with maize genomic DNA to identify these rDNA containing clones. I am not sure about the amount of labelled probe to use for this HYB. For a normal Southern with a certain fragment as probe i use 50-100ng (P32-labelling). But is this enough to cover all species of rDNA within genomic DNA? Thanks for your answers in advance, Marcel ---