From owner-diagnost@hgmp.mrc.ac.uk Tue Aug 20 10:21:56 2002 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id D9D0717BFB for ; Tue, 20 Aug 2002 10:21:53 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 1707717BE8 for ; Tue, 20 Aug 2002 10:21:48 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6045) id E3EED17C00; Tue, 20 Aug 2002 10:19:49 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 3B63617D36 for ; Tue, 6 Aug 2002 18:36:51 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id EBB4217C53; Tue, 6 Aug 2002 18:36:45 +0100 (BST) To: bionet-diagnostics@net.bio.net From: Alan.Wheeler@gov.ky ("Wheeler, Alan") Newsgroups: bionet.diagnostics Subject: Question about agarose gel electrophoresis Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <4F3534137955D3119C2400508B0EC60A07353FEE@SVREXCH> MIME-Version: 1.0 Content-Type: multipart/alternative; boundary="----_=_NextPart_001_01C23D6F.C6675480" X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 660D317D37 for ; Tue, 6 Aug 2002 18:36:43 +0100 (BST) X-Received: from svrexch.exchange.gov.ky (unknown [209.27.55.193]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id C6F5117C53 for ; Tue, 6 Aug 2002 18:36:34 +0100 (BST) X-Received: by SVREXCH with Internet Mail Service (5.5.2653.19) id ; Tue, 6 Aug 2002 12:36:26 -0500 X-To: "'diagnost@net.bio.net'" X-Mailer: Internet Mail Service (5.5.2653.19) Date: Tue, 20 Aug 2002 10:19:49 +0100 (BST) X-Razor-id: 6470471b91e884822915aacf119d5da58a04788d Sender: owner-diagnost@hgmp.mrc.ac.uk Precedence: bulk This message is in MIME format. Since your mail reader does not understand this format, some or all of this message may not be legible. ------_=_NextPart_001_01C23D6F.C6675480 Content-Type: text/plain; charset="iso-8859-1" I am just starting to monitor for West Nile Virus using PCR. I expect to be getting a large number of negative samples. I am wondering if it is possible to re-use a gel if all the samples run in it come up negative. What I was thinking of doing would be to prepare 24 samples and run them three times in the same gel. Please send any replies directly to me because I have not yet signed up to this group. regards Alan Disclaimer The contents of this e-mail are confidential to the addressee and are intended solely for their exclusive use. If you are not the addressee, you have received this e-mail in error and any disclosure, copying, distribution or action taken in reliance on it is prohibited and may be unlawful. Any opinions expressed in this e-mail are those of the author and do not necessarily reflect those of the Cayman Islands Government or anyone associated thereof. Visit the official website of the Cayman Islands Government at http://www.gov.ky ------_=_NextPart_001_01C23D6F.C6675480 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Question about agarose gel electrophoresis

I am just starting to monitor for West = Nile Virus using PCR. I expect to be getting a large number of negative = samples. I am wondering if it is possible to re-use a gel if all the = samples run in it come up negative. What I was thinking of doing would = be to prepare 24 samples and run them three times in the same gel. =

Please send any replies directly to me = because I have not yet signed up to this group.

regards

Alan

Disclaimer

The contents of this e-mail are = confidential to the addressee and are intended solely for their = exclusive use. If you are not the addressee, you have received this = e-mail in error and any disclosure, copying, distribution or action = taken in reliance on it is prohibited and may be unlawful.

Any opinions expressed in this e-mail = are those of the author and do not necessarily reflect those of the = Cayman Islands Government or anyone associated thereof.

Visit the official website of the = Cayman Islands Government at http://www.gov.ky=20

------_=_NextPart_001_01C23D6F.C6675480-- --- From owner-diagnost@hgmp.mrc.ac.uk Tue Aug 20 10:22:33 2002 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 06A9317C01 for ; Tue, 20 Aug 2002 10:22:29 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 9272417BF0 for ; Tue, 20 Aug 2002 10:22:24 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6045) id 6E1F717C04; Tue, 20 Aug 2002 10:20:23 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 718DA17E5F for ; Wed, 7 Aug 2002 17:01:14 +0100 (BST) Received: from birch.cc.uic.edu (birch.cc.uic.edu [128.248.155.162]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 77B7817F64 for ; Wed, 7 Aug 2002 16:06:47 +0100 (BST) Received: (qmail 865 invoked from network); 7 Aug 2002 10:06:30 -0500 Received: from tigger.cc.uic.edu (128.248.155.51) by birch.cc.uic.edu with SMTP; 7 Aug 2002 10:06:30 -0500 From: Padmaja Vellanki X-Sender: padmaja@tigger.cc.uic.edu To: bionet-diagnostics@net.bio.net Subject: mycoplasma contamination - protein expresssion Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Newsgroups: bionet.diagnostics Date: Tue, 20 Aug 2002 10:20:23 +0100 (BST) X-Razor-id: eba6638a3ec982aaa5711b8fbef83818cac2591f Sender: owner-diagnost@hgmp.mrc.ac.uk Precedence: bulk Can anyone explain how mycoplasma contamination can affect protein expression in Eukaryotic system?? Also, suggest me the best Eukaryotic system for recombinant protein production in large scale. thaks padmaja