From owner-diagnost@hgmp.mrc.ac.uk Wed Feb 5 14:33:19 2003 Return-Path: X-Original-To: diagnost-outgoing Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 910477D140 for ; Wed, 5 Feb 2003 14:33:19 +0000 (GMT) X-Original-To: diagnost-list@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 022547D288 for ; Wed, 5 Feb 2003 14:33:19 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6045) id 531CC7D140; Wed, 5 Feb 2003 14:33:18 +0000 (GMT) X-Original-To: diagnost-submit@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 56C187D0C7 for ; Mon, 13 Jan 2003 15:55:56 +0000 (GMT) Received: from mailbox4.ucsd.edu (mailbox4.ucsd.edu [132.239.1.56]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 3DA637D13C for ; Mon, 13 Jan 2003 15:55:50 +0000 (GMT) Received: from proxy.google.com (proxy.google.com [216.239.35.5]) by mailbox4.ucsd.edu (8.12.3/8.12.3) with ESMTP id h0DF32GS020055 for ; Mon, 13 Jan 2003 07:03:02 -0800 (PST) Received: from sjmm36.sj.google.com (sjmm36.prod.google.com [10.6.39.36]) by proxy.google.com (8.12.6/8.12.3) with ESMTP id h0DF2rib009363 for ; Mon, 13 Jan 2003 07:02:53 -0800 Received: (from news@localhost) by sjmm36.sj.google.com (8.12.6/8.12.3) id h0DF2rej019114 for bionet-diagnostics@moderators.isc.org; Mon, 13 Jan 2003 07:02:53 -0800 To: bionet-diagnostics@moderators.isc.org From: tony.oconnor@may.ie (Tony O'Connor) Newsgroups: bionet.cellbiol,bionet.molbio.methds-reagnts,bionet.diagnostics,bionet.immunology Subject: biotinylated protein for amplification Organization: http://groups.google.com/ Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Trace: posting.google.com 1042470173 19113 127.0.0.1 (13 Jan 2003 15:02:53 GMT) X-Complaints-To: groups-abuse@google.com NNTP-Posting-Date: 13 Jan 2003 15:02:53 GMT Date: Wed, 5 Feb 2003 14:33:18 +0000 (GMT) Sender: owner-diagnost@hgmp.mrc.ac.uk Precedence: bulk I want to biotinylate a protein to use as an "amplification layer" in an ELISA, as follows: [Solid-phase]-(Ab)-(Ag)-(biotinylated Ab)-(streptavidin)- *{biotinylated protein}n*-(HRP-streptavidin)n I'm doing this to replace a biotinylated anti-streptavidin Ab which is not amplifying the signal. I'm going to try BSA - there's always BSA in the lab :-) Has anyone tried other proteins (adequately-sized with several non-adjacent accessible amino groups) in such a system? Thanks, Tony Tony O'Connor Institute of Immunology NUI Maynooth Ireland tony.oconnor@may.ie From owner-diagnost@hgmp.mrc.ac.uk Wed Feb 5 14:33:52 2003 Return-Path: X-Original-To: diagnost-outgoing Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 9D9057D29E for ; Wed, 5 Feb 2003 14:33:52 +0000 (GMT) X-Original-To: diagnost-list@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 2D7097D140 for ; Wed, 5 Feb 2003 14:33:52 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6045) id 283357D211; Wed, 5 Feb 2003 14:33:51 +0000 (GMT) X-Original-To: diagnost-submit@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 2EEA67D31F for ; Sat, 18 Jan 2003 18:09:57 +0000 (GMT) Received: from chx400.switch.ch (unknown [130.59.10.2]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 08B1F7D31E for ; Sat, 18 Jan 2003 18:09:57 +0000 (GMT) Received: from clmboh1-nws3-ge0.columbus.rr.com ([65.24.3.198] helo=sandstorm.neo.rr.com) by chx400.switch.ch with esmtp (Exim 3.20 #1) id 18ZxPo-0001Kd-00 for bionet-diagnostics@moderators.isc.org; Sat, 18 Jan 2003 19:09:56 +0100 Received: (from news@localhost) by sandstorm.neo.rr.com (8.8.8+Sun/8.8.8) id NAA00645; Sat, 18 Jan 2003 13:16:52 -0500 (EST) To: bionet-diagnostics@moderators.isc.org Subject: test From: joe user Reply-To: chopkins Newsgroups: bionet.diagnostics Message-ID: X-Complaints-To: abuse@rr.com X-Trace: twister.neo.rr.com 1042913812 24.166.34.144 (Sat, 18 Jan 2003 13:16:52 EST) NNTP-Posting-Date: Sat, 18 Jan 2003 13:16:52 EST Organization: Road Runner High Speed Online -- Northeast Ohio Date: Wed, 5 Feb 2003 14:33:51 +0000 (GMT) Sender: owner-diagnost@hgmp.mrc.ac.uk Precedence: bulk 0123456789 From owner-diagnost@hgmp.mrc.ac.uk Mon Feb 10 10:50:44 2003 Return-Path: X-Original-To: diagnost-outgoing Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 195AA7D13B for ; Mon, 10 Feb 2003 10:50:44 +0000 (GMT) X-Original-To: diagnost-list@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 6B0217D1B1 for ; Mon, 10 Feb 2003 10:50:43 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6045) id D424B7D13B; Mon, 10 Feb 2003 10:50:42 +0000 (GMT) X-Original-To: diagnost-submit@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 970F07D292 for ; Fri, 7 Feb 2003 15:08:26 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 728547D273; Fri, 7 Feb 2003 15:08:26 +0000 (GMT) To: bionet-diagnostics@net.bio.net From: hiv2003@club-internet.fr (Int.Persistence.Workshop) Newsgroups: bionet.diagnostics Subject: International HIV Persistence Workshop Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <20030207150823.70ECBE0EE@relay-4m.club-internet.fr> Mime-Version: 1.0 Content-Type: text/html; charset=us-ascii X-Original-To: diagnost@hgmp.mrc.ac.uk X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 077297D29A for ; Fri, 7 Feb 2003 15:08:25 +0000 (GMT) X-Received: from relay-4m.club-internet.fr (relay-4m.club-internet.fr [194.158.104.43]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id A6FA47D0EE for ; Fri, 7 Feb 2003 15:08:24 +0000 (GMT) X-Received: from clubint/avps (lven1-2-227.n.club-internet.fr [213.44.5.227]) by relay-4m.club-internet.fr (Postfix) with SMTP id 70ECBE0EE for ; Fri, 7 Feb 2003 16:08:23 +0100 (CET) X-To: diagnost@net.bio.net X-Reply-To: kapoul@club-internet.fr Date: Mon, 10 Feb 2003 10:50:42 +0000 (GMT) Sender: owner-diagnost@hgmp.mrc.ac.uk Precedence: bulk
 

First International Workshop on HIV Persistence during Therapy

Saint Martin, FWI, 10-12 December 2003
Updated Information Available on the Meeting Website




You can find updated information on this Workshop on the Website including the advanced program. The deadline for abstract submission is: May 15, 2003.


Chalucet Hospital
TOULON   83056

Email: hiv2003@club-internet.fr


URL: http://www.avps.org/2003/hivp.htm

To unsubscribe send an email to kapoul@club-internet.fr

 
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