From owner-diagnost@hgmp.mrc.ac.uk Mon Jul 5 16:45:43 2004 Return-Path: X-Original-To: diagnost-outgoing Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 5CD667D123 for ; Mon, 5 Jul 2004 16:45:43 +0100 (BST) X-Original-To: diagnost-list@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id DBB8C7D0FF for ; Mon, 5 Jul 2004 16:45:42 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6045) id A726D7D123; Mon, 5 Jul 2004 16:45:35 +0100 (BST) X-Original-To: bionet-diagnostics@net.bio.net Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 411CA7D34E; Wed, 26 May 2004 15:15:18 +0100 (BST) Received: from dexter.wzw.tum.de (dexter.wzw.tum.de [141.40.103.101]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 6BC6C7D347; Wed, 26 May 2004 15:15:10 +0100 (BST) Received: from pfaffl.wzw.tum.de ([141.40.217.71]) by dexter.wzw.tum.de (8.12.6/8.12.6/SuSE Linux 0.6) with ESMTP id i4QEF9dL000257; Wed, 26 May 2004 16:15:09 +0200 Message-Id: <5.2.1.1.0.20040526161642.028f7e70@pollux.wzw.tum.de> X-Sender: genequan@pollux.wzw.tum.de X-Mailer: QUALCOMM Windows Eudora Version 5.2.1 To: bio-srs@net.bio.net, bionauts@net.bio.net, cellbiol@net.bio.net, immuno@net.bio.net, molmodel@net.bio.net, microbio@net.bio.net, fluorpro@net.bio.net, bionet-diagnostics@net.bio.net, btechniq@net.bio.net, bionews@net.bio.net, bio-www@net.bio.net, 7tms_r@net.bio.net, zbrafish@net.bio.net, x-plor@net.bio.net, recom@net.bio.net, prenatal@net.bio.net, plant-ed@net.bio.net, phbiotec@net.bio.net, mol-evol@net.bio.net From: "qPCR.Gene-Quantification.Info Newsletter" Subject: May 2004 Mime-Version: 1.0 Content-Type: multipart/alternative; boundary="=====================_26938062==.ALT" Newsgroups: bionet.diagnostics Date: Mon, 5 Jul 2004 16:45:35 +0100 (BST) Sender: owner-diagnost@hgmp.mrc.ac.uk Precedence: bulk --=====================_26938062==.ALT Content-Type: text/plain; charset="iso-8859-1"; format=flowed Content-Transfer-Encoding: quoted-printable Gene Quantification Newsletter May 2004 A new URL is defined for the Gene Quantification home page http://www.gene-quantification.info A lot of NEWS are presented: On HKG and normalization page: Guideline to reference gene selection for quantitative real-time PCR Selection of appropriate control genes to assess expression of tumor=20 antigens using real-time RT-PCR Comparison of RiboGreen=AE and 18S rRNA quantitation for normalizing=20 real-time RT-PCR expression analysis Novel Internal Controls For Real-Time PCR Assays Selection of optimal internal controls for gene expression profiling of=20 liver disease On real-time PCR page: Real-time PCR in the microbiology laboratory Quantitative RT-PCR: pitfalls and potential Real-time RT-PCR: Neue Ans=E4tze zur exakten mRNA Quantifizierung On chemistry page: http://dyes.gene-quantification.info/ Short summary of detection formats SYBR Green I BEBO TaqMan Probes LightCycler Hybridisation Probes Molecular Beacons Scorpions LNA Primers Light-Up Probes Q Zyme Assay Amplifluor Quantitative PCR Detection System LUX (Light Upon eXtension) primers The iCycler iQ Detection System for multiplex real-time PCR Assays A novel real-time quantitative PCR method using attached universal template= =20 probe Determination of real-time PCR efficiency All necessary informations about qPCR efficiency estimation http://efficiency.gene-quantifi= cation.info BioInformatics in real-time PCR A lot of applications related to qPCR or real-time PCR STATISTICS AND GENE EXPRESSION ANALYSIS by TERRY SPEED http://bioinformatics.gene-= quantification.info Endocrinology, Physiology and Immunology Application of real-time PCR in molecular Physiology, molecular=20 Endocrinology and molecular Immunology http://physiology.gene-quantifi= cation.info The 1st international qPCR Symposium & Application Workshop Please look at the nice pictures of the Symposium http://qpcr2004.gene-quantificati= on.info and Workshop talks are FREE for download http://qpcr= 2004.gene-quantification.info/qpcr2004-workshop.html Please send the NEWSLETTER and the=20 http://www.gene-quantification.info=20 home page links to further scientists and friends who are interested in=20 real-time PCR, new quantification strategies, relative or absolute gene=20 quantification using kinetic PCR. best regards Michael Pfaffl ############################################# For further questions concerning real-time PCR and if you want to SUBSCRIBE= =20 to our monthly appearing GENE QUANTIFICATION NEWSLETTER please contact=20 gene.quantification@wzw.tum.de You= =20 will receive all new features and future REST & REST-XL & BestKeeper=20 updates. GENE QUANTIFICATION web page and the GENE QUANTIFICATION NEWSLETTER are=20 educational sites with the only purpose of facilitating access to=20 biology-related information over the internet. The web page is optimised=20 for Netscape Navigator version 4.7 & Internet Explorer version 5.0 (and=20 higher versions) with an optimal screen resolution of 1024 x 768 pixels. Disclaimer & copyright on=20 http://www.gene-quantification.info=20 homepage. ******************************************************************** www.Gene-Quantification.Info mail: gene.quantification@wzw.tum.de homepage: http://www.gene-quantification.info PD Dr. Michael W. Pfaffl Physiology - Weihenstephan Life Science Center Weihenstephan, Technical University of Munich Weihenstephaner Berg 3 D-85350 Freising-Weihenstephan, GERMANY ******************************************************************** =20 --=====================_26938062==.ALT Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable

Gene Quantification Newsletter May 2004

A new URL is defined for the Gene Quantification home page

http://www.gene-quantificatio= n.info
 

A lot of NEWS are presented:

On HKG and normalization page:
Guideline to reference gene selection for quantitative real-time PCR
Selection of appropriate control genes to assess expression of tumor antigens using real-time RT-PCR
Comparison of RiboGreen=AE and 18S rRNA quantitation for normalizing real-time RT-PCR expression analysis
Novel Internal Controls For Real-Time PCR Assays
Selection of optimal internal controls for gene expression profiling of liver disease

On real-time PCR page:
Real-time PCR in the microbiology laboratory
Quantitative RT-PCR: pitfalls and potential
Real-time RT-PCR: Neue Ans=E4tze zur exakten mRNA Quantifizierung

On chemistry page:
http://dyes.gene-quantificat= ion.info/

Short summary of detection formats
SYBR Green I
BEBO
TaqMan Probes
LightCycler Hybridisation Probes
Molecular Beacons
Scorpions
LNA Primers
Light-Up Probes
Q Zyme Assay
Amplifluor Quantitative PCR Detection System
LUX (Light Upon eXtension) primers
The iCycler iQ Detection System for multiplex real-time PCR Assays
A novel real-time quantitative PCR method using attached universal template probe


Determination of real-time PCR efficiency

All necessary informations about qPCR efficiency estimation
http://efficiency.gene= -quantification.info

BioInformatics in real-time PCR

A lot of applications related to qPCR or real-time PCR
STATISTICS AND GENE EXPRESSION ANALYSIS by TERRY SPEED
http://bioinformat= ics.gene-quantification.info


Endocrinology, Physiology and Immunology

Application of real-time PCR in molecular Physiology, molecular Endocrinology and molecular Immunology
http://physiology.gene= -quantification.info


The 1st international qPCR Symposium & Application Workshop

Please look at the nice pictures of the Symposium
http://qpcr2004.gene-qua= ntification.info
and Workshop talks are FREE for download
ht= tp://qpcr2004.gene-quantification.info/qpcr2004-workshop.html

 
Please send the NEWSLETTER and the http://www.gene-quantificat= ion.info home page links to further scientists and friends who are interested in= real-time PCR, new quantification strategies, relative or absolute gene= quantification using kinetic PCR.

best regards

Michael Pfaffl

#############################################

For further questions concerning real-time PCR and if= you want to SUBSCRIBE to our monthly appearing GENE QUANTIFICATION= NEWSLETTER please contact gene.quantification@wzw.tum.= de   You will receive all new features and future REST= &  REST-XL  &  BestKeeper updates.

GENE QUANTIFICATION web page and the GENE QUANTIFICATION NEWSLETTER are= educational sites with the only purpose of facilitating access to= biology-related information over the internet. The web page is optimised= for Netscape Navigator version 4.7 & Internet Explorer version 5.0 (and= higher versions) with an optimal screen resolution of 1024 x 768= pixels.
 
Disclaimer & copyright on http://www.gene-quantificatio= n.info homepage.

******************************************************************= **
www.Gene-Quantification.Info

mail:       = ;    gene.quantification@wzw.tum.de
homepage:       http://www.gene-quantification.info

PD Dr. Michael W. Pfaffl
Physiology - Weihenstephan
Life Science Center Weihenstephan,
Technical University of Munich
Weihenstephaner Berg 3
D-85350 Freising-Weihenstephan, GERMANY
******************************************************************= ** --=====================_26938062==.ALT-- From owner-diagnost@hgmp.mrc.ac.uk Mon Jul 5 16:48:15 2004 Return-Path: X-Original-To: diagnost-outgoing Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 42CC47D128 for ; Mon, 5 Jul 2004 16:48:15 +0100 (BST) X-Original-To: diagnost-list@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 7EF6C7D126 for ; Mon, 5 Jul 2004 16:48:14 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6045) id 054C37D128; Mon, 5 Jul 2004 16:48:12 +0100 (BST) X-Original-To: bionet-diagnostics@net.bio.net Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id A620E7D1C6 for ; Mon, 7 Jun 2004 06:21:30 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 8D3057D0EF; Mon, 7 Jun 2004 06:21:29 +0100 (BST) To: bionet-diagnostics@net.bio.net From: DAM8U@hscmail.mcc.virginia.edu ("Moody, David *HS") Newsgroups: bionet.diagnostics Subject: lab calculations Organization: BIOSCI/MRC Rosalind Franklin Centre for Genomics Research Message-ID: content-class: urn:content-classes:message MIME-Version: 1.0 Content-Type: multipart/alternative; boundary="----_=_NextPart_001_01C44C4F.46911CC5" X-Original-To: diagnost@net.bio.net X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 3E6B77D148 for ; Mon, 7 Jun 2004 06:21:28 +0100 (BST) X-Received: from HSCEMAIL5.hscs.virginia.edu (hscemail5.hscs.Virginia.EDU [128.143.176.152]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 454577D0EF for ; Mon, 7 Jun 2004 06:21:25 +0100 (BST) X-Received: from HSCSEMAIL3.hscs.virginia.edu ([128.143.165.14]) by HSCEMAIL5.hscs.virginia.edu with Microsoft SMTPSVC(5.0.2195.6713); Mon, 7 Jun 2004 01:19:23 -0400 X-MimeOLE: Produced By Microsoft Exchange V6.0.6487.1 X-MS-Has-Attach: X-MS-TNEF-Correlator: X-Thread-Topic: lab calculations X-Thread-Index: AcRMT0MiM17tUNS6RJKDbea/vPzXXw== X-To: X-OriginalArrivalTime: 07 Jun 2004 05:19:23.0687 (UTC) FILETIME=[FEA91370:01C44C4E] Date: Mon, 5 Jul 2004 16:48:12 +0100 (BST) Sender: owner-diagnost@hgmp.mrc.ac.uk Precedence: bulk This is a multi-part message in MIME format. ------_=_NextPart_001_01C44C4F.46911CC5 Content-Type: text/plain; charset="us-ascii" Content-Transfer-Encoding: quoted-printable Hello, Does anyone know of a software tool that can help streamline tedious dilution and other common lab calculations? I found this one at http://www.solutionsolver.com , it seems pretty good, and has a free version. Any thoughts? I am not looking to spend much money. ------_=_NextPart_001_01C44C4F.46911CC5 Content-Type: text/html; charset="us-ascii" Content-Transfer-Encoding: quoted-printable lab calculations

Hello,

Does = anyone know of a software tool that can help streamline tedious dilution = and other common lab calculations? I found this one at http://www.solutionsolver.com = , it seems pretty good, and has a free version. Any thoughts? I am not = looking to spend much money.

------_=_NextPart_001_01C44C4F.46911CC5-- --- From owner-diagnost@hgmp.mrc.ac.uk Mon Jul 5 16:48:19 2004 Return-Path: X-Original-To: diagnost-outgoing Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 4EADD7D12C for ; Mon, 5 Jul 2004 16:48:19 +0100 (BST) X-Original-To: diagnost-list@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 9BC9F7D12D for ; Mon, 5 Jul 2004 16:48:18 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6045) id A48097D12C; Mon, 5 Jul 2004 16:48:13 +0100 (BST) X-Original-To: bionet-diagnostics@net.bio.net Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 0C7FC7D12B for ; Wed, 9 Jun 2004 00:48:51 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 9FAC47D105; Wed, 9 Jun 2004 00:48:50 +0100 (BST) To: bionet-diagnostics@net.bio.net From: americanbiotechnology@earthlink.net Newsgroups: bionet.diagnostics Subject: American Biotechnology News Organization: BIOSCI/MRC Rosalind Franklin Centre for Genomics Research Message-ID: <20040608164839.AEDB84967F6377DE@earthlink.net> charset="iso-8859-1" X-Original-To: diagnost@net.bio.net X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id D23CD7D12B for ; Wed, 9 Jun 2004 00:48:49 +0100 (BST) X-Received: from earthlink.net (unknown [63.236.35.133]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 76A637D105 for ; Wed, 9 Jun 2004 00:48:47 +0100 (BST) X-To: diagnost@net.bio.net X-Reply-To: americanbiotechnology@earthlink.net Date: Mon, 5 Jul 2004 16:48:13 +0100 (BST) Sender: owner-diagnost@hgmp.mrc.ac.uk Precedence: bulk Dear Colleague,

Dear Colleague,

Please accept my invitation to receive a free subscription to our journal, American Biotechnology Laboratory.

American Biotechnology Laboratory is the leading life science tabloid publication that includes news and information about the latest laboratory products. Each issue features articles submitted by prominent scientists from around the world reviewing laboratory techniques and applications of instrumentation and software.

For your FREE subscription to American Biotechnology Laboratory please respond to this email or return by fax to 408-446-0771 with the following data:

 

 

 

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