From owner-embldatabank@net.bio.net Fri Aug 11 23:00:00 1995
Path: biosci!internet!biosci!not-for-mail
From: biohelp (BIOSCI Administrator)
Newsgroups: bionet.molbio.embldatabank
Subject: UNSUBSCRIBING, BIOSCI ARCHIVES, ADDRESS DATABASE & BIOSCI FAQ
Date: 12 Aug 1995 02:00:42 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 347
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199508120900.CAA11786@net.bio.net>
NNTP-Posting-Host: net.bio.net


Four important items follow: How to cancel e-mail subscriptions to
BIOSCI newsgroups, BIOSCI archive searching, the BIOSCI FAQ, and the
BIOSCI User Address Directory form.  If you have not yet listed
yourself in our BIOSCI user directory, please take a few minutes to
complete and return the form below.  If your personal information has
changed since you listed yourself, please send us a complete new
updated form.  We can not make manual revisions to existing entries.

				Sincerely,

				Dave Kristofferson
				BIOSCI/bionet Manager

				biosci-help@net.bio.net



	 **** How to cancel a BIOSCI e-mail subscription ****

If you want to cancel your e-mail subscription to this group, 
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This would send your request to all of the readers of the newsgroup,
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If you need personal assistance, a BIOSCI staff member can be
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Support Address                      Location
---------------                      --------
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biosci-help@net.bio.net              Americas and the Pacific Rim


		 **** SEARCHING BIOSCI ARCHIVES ****

The easiest way to search the BIOSCI archives is to use Mosaic or
another World Wide Web browser and connect to the BIOSCI WWW home page
at URL http://www.bio.net/.  Select the hypertext link to the BIOSCI
archives.  This gives you read access to all newsgroup messages and
the ability to search the indexes described below.

You can also use gopher software and connect over the Internet to
net.bio.net, the U.S. BIOSCI computer.  We maintain three indexes
which are searchable from the main gopher menu on net.bio.net: (1) an
index of all BIOSCI postings; (2) an index of individual journal
article references from the Table of Contents postings on the
BIO-JOURNALS newsgroup; and (3) an index of BIOSCI users including
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interests, and newsgroup participation.

E-mail users can search the BIOSCI archives by using our waismail
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help

to waismail@net.bio.net.  Leave the Subject: line blank (anything
entered on the Subject: line is ignored).

WAIS software can also be used to search the archives as described in
the BIOSCI FAQ (see below).

Finally, the BIOSCI archive files are accessible by anonymous FTP to
net.bio.net [134.172.2.69] in the directory pub/BIOSCI.


       **** BIOSCI FREQUENTLY ASKED QUESTIONS (FAQ) SHEET ****

New users of BIOSCI/bionet may want to read the "Frequently Asked
Questions" or "FAQ" sheet for BIOSCI.  The FAQ provides details on how
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documentation is available through our WWW home page at URL
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by sending the command

info faq

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The FAQ is also posted on the first of each month to the newsgroup
BIONEWS/bionet.announce immediately following the posting of the
BIOSCI information sheet.


	       **** BIOSCI USER ADDRESS DIRECTORY ****

Please take this opportunity to add your name and address information
to the BIOSCI User Address Database if you have not already done so.

Below is the address form that we would like each reader of the
BIOSCI/bionet newsgroups to complete and return if you would like to
be listed in our database.  The database serves as a directory that
enables biologists, who are currently using (or even just reading) the
BIOSCI newsgroups, to look up e-mail addresses and other information
about our users.

The address database is reindexed nightly for WAIS, waismail, gopher,
and WWW access (the URL is http://www.bio.net).  If you have access to
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access to WAIS, please use our WAIS source biologists-addresses.src.
If you are not on the Internet, please use our waismail server (send
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command in the body of the mail message.).

Please carefully follow the instructions for completing the form
below and return it to either of the following two addresses
(whichever is more convenient for you).  Thanks in advance for taking
the time to complete and return the form.

Addresses for returning forms         Location        Network
-----------------------------         --------        -------
biovote@net.bio.net                   U.S.A.          Internet/BITNET
biovote@daresbury.ac.uk               U.K.            JANET


	     MAKING SURE THAT YOUR INFORMATION IS CURRENT

This notice will be mailed bimonthly to each newsgroup.  You should
check your database entry from time-to-time to see if your address
information is still up-to-date.


		  Using Gopher to complete the form
                  ---------------------------------

If you don't want to use a text editor, you can also use Dan
Jacobson's gopher site to fill out the address database form as
follows.  Otherwise skip this section on gopher and proceed to the
instructions for filling out the form below.

> To add yourself to the database just point your
> gopher client at merlot.gdb.org and select the following:
> 
> -->  14. Searching For Biologists/
> 
>  -->  9.  E-mail Addresses of Biosci-Bionet Users/
> 
>   -->  1.  Add (or Correct) Your Address to the BIOSCI User Address
> Data..
> 
> 
> And fill out the form.

or Rob Harper's gopher site in Europe as follows:

> Europeans can point their gopher client at gopher.csc.fi and add their
> information to the database. All entries will be mailed directly to
> Dave for incorporation in a wais source.
> 
> The path to the questionare is as follows.
> 
> 
> 6.  Information in English/
> 
>     5.  Scientific and other topics/
> 
>         1.  Finnish EMBnet BioBox/
> 
>             9.  FAQ Files/
> 
>                 5.  Bionauts Address Database (questionaire) <TEL>
> 



	    IMPORTANT INSTRUCTIONS - PLEASE READ CAREFULLY

Please enter all responses after the : on each line, leaving one (1)
blank space after the : (i.e., before the start of your text).

Please do NOT extend your responses past the end of each line (80
characters).

PLEASE DO NOT alter any of the field identifiers such as "first name: ". 
If you have nothing to enter after a field identifier, PLEASE LEAVE IT
- do not delete it even if there is no data on the line in question.

Several lines are provided at the end of the form for comments, but,
please adhere to the line length restriction.

On the date: line, please enter the date in the DD-MM-YY format, e.g.,
15-05-93 for 15 May 1993.  This line will tell others when the
information was last updated.  Please be sure to include the 0's for
single digit days or months, e.g., 15-05-93, not 15-5-93.

Note that the "e-mail network: " line below is for specifying, e.g.,
"Internet," "BITNET," "EARN," "JANET," or whatever other network that
your computer may be on.

If you are uncertain about any field, please feel free to leave it
blank, but please DO NOT DELETE the field identifier from the form!

In the first field below, "New information or Update ...", please
enter "N" if this is the first time that you have registered in the
directory or "U" if you are correcting a listing that you sent to us
previously.

The comment: lines may be used for anything that you like but PLEASE
DO NOT DELETE THEM FROM THE FORM OR ALTER THEM.  One suggested use is
to list the names of the newsgroups in which you participate.  Please
use the MAILING LIST name (see below - the latest version of the list
can be requested from biosci@net.bio.net) instead of the USENET name
even if you don't participate by e-mail.  WAIS might get confused by
the periods in the USENET names.  This allows one to retrieve via WAIS
or waismail the list of participants in a particular group.

For example:

comment: ARABIDOPSIS PLANT-BIOLOGY BIONEWS

On the comment: lines
use these names below ---- NOT the USENET names below

MAILING LIST NAME          USENET Newsgroup Name
-----------------          ---------------------
ACEDB-SOFT                 bionet.software.acedb
AGEING                     bionet.molbio.ageing
AGROFORESTRY               bionet.agroforestry
ARABIDOPSIS                bionet.genome.arabidopsis
ASCB                       bionet.prof-society.ascb
BIOCAN                     bionet.prof-society.cfbs
BIOFORUM                   bionet.general
BIO-INFORMATION-THEORY     bionet.info-theory
BIONAUTS                   bionet.users.addresses
BIONEWS                    bionet.announce
BIO-JOURNALS               bionet.journals.contents
BIO-MATRIX                 bionet.molbio.bio-matrix
BIOPHYSICAL-SOCIETY        bionet.prof-society.biophysics
BIOPHYSICS                 bionet.biophysics
BIO-SOFTWARE               bionet.software
BIOTHERMOKINETICS          bionet.metabolic-reg
BIO-WWW                    bionet.software.www
CARDIOVASCULAR-RESEARCH    bionet.biology.cardiovascular
CELEGANS                   bionet.celegans
CELL-BIOLOGY               bionet.cellbiol
CHLAMYDOMONAS              bionet.chlamydomonas
CHROMOSOMES                bionet.genome.chromosomes
COMPUTATIONAL-BIOLOGY      bionet.biology.computational
CSM                        bionet.prof-society.csm
CYTONET                    bionet.cellbiol.cytonet
DROSOPHILA                 bionet.drosophila
EMBL-DATABANK              bionet.molbio.embldatabank
EMF-BIO                    bionet.emf-bio
EMPLOYMENT                 bionet.jobs
EMPLOYMENT-WANTED          bionet.jobs.wanted
FASEB                      bionet.prof-society.faseb
GDB                        bionet.molbio.gdb
GENBANK-BB                 bionet.molbio.genbank
GENETIC-LINKAGE            bionet.molbio.gene-linkage
GRASSES-SCIENCE            bionet.biology.grasses
HIV-MOLECULAR-BIOLOGY      bionet.molbio.hiv
HUMAN-GENOME-PROGRAM       bionet.molbio.genome-program
IMMUNOLOGY                 bionet.immunology
INFO-GCG                   bionet.software.gcg
JOURNAL-NOTES              bionet.journals.note
METHODS-AND-REAGENTS       bionet.molbio.methds-reagnts
MICROBIOLOGY               bionet.microbiology
MOLECULAR-EVOLUTION        bionet.molbio.evolution
MOLECULAR-MODELLING        bionet.molec-model
MOLLUSC-MOLECULAR-NEWS     bionet.molbio.molluscs
MYCOLOGY                   bionet.mycology
NEUROSCIENCE               bionet.neuroscience
N2-FIXATION                bionet.biology.n2-fixation
PARASITOLOGY               bionet.parasitology
PHOTOSYNTHESIS             bionet.photosynthesis
PLANT-BIOLOGY              bionet.plants
POPULATION-BIOLOGY         bionet.population-bio
PROTEIN-ANALYSIS           bionet.molbio.proteins
PROTEIN-CRYSTALLOGRAPHY    bionet.xtallography
PROTISTA                   bionet.protista
RAPD                       bionet.molbio.rapd
SCIENCE-RESOURCES          bionet.sci-resources
STADEN                     bionet.software.staden
STRUCTURAL-NMR             bionet.structural-nmr
TROPICAL-BIOLOGY           bionet.biology.tropical
URODELES                   bionet.organisms.urodeles
VIROLOGY                   bionet.virology
WOMEN-IN-BIOLOGY           bionet.women-in-bio
YEAST                      bionet.molbio.yeast
ZBRAFISH                   bionet.organisms.zebrafish

Listing newsgroups on the comment: line is optional, of course.

Thanks again for your cooperation!



--------------- please cut here and return portion below ---------------

New information or Update to old record (enter N or U): 
date (DD-MM-YY): 
first name: 
middle initial: 
family name: 
job title: 
e-mail address: 
e-mail network: 
phone number: 
FAX number: 
institution: 
address1: 
address2: 
address3: 
city: 
state/province: 
country: 
postal code: 
research interest: 
research interest: 
comment: 
comment: 
comment: 
comment: 
comment: 


From owner-embldatabank@net.bio.net Thu Aug 17 23:00:00 1995
Path: biosci!bcm!cs.utexas.edu!swrinde!tank.news.pipex.net!pipex!warwick!news.shef.ac.uk!newsmaster@sheffield
From: "D.J.Cant" <D.J.Cant@shef.ac.uk>
Newsgroups: bionet.molbio.embldatabank
Subject: Help!  I need some addresses! Email and Postal!
Date: 18 Aug 1995 12:24:32 GMT
Organization: Medicine/dentistry, University of Sheffield , UK
Lines: 21
Message-ID: <4120q0$9nj@hippo.shef.ac.uk>
NNTP-Posting-Host: pc077042.shef.ac.uk
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 1.1N (Windows; I; 16bit)

I am looking for the addresses to the following institutes:

(If there is an Email address to contact them with, then so much the 
better.)

         MRC (England)
         ICRF (England)
         EMBL (Germany)
         Max Planck Inst. (Germany)
         Biozentrum (Switzerland)
         F. Miescher Inst. (Switzerland)

If as in some cases, there may be more than one address, i would likle to 
have all of them.  I wouold be extremely appreciative of anyone who can 
help me.  Please use my Email address to contact me.



                 Charles Cant.



From owner-embldatabank@net.bio.net Mon Aug 21 23:00:00 1995
Path: biosci!bcm!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!EU.net!Germany.EU.net!news.dfn.de!fu-berlin.de!news.belwue.de!News.Uni-Marburg.DE!news.th-darmstadt.de!fauern!gs.dfn.de!immunbio.mpg.de!immunbio.mpg.de!news
Newsgroups: bionet.molbio.embldatabank
Subject: Re: Help!  I need some addresses! Email and Postal!
Message-ID: <1995Aug21.215844.196@immunbio.mpg.de>
From: GARTMANN@IMMUNBIO.MPG.DE (Christoph Gartmann)
Date: 21 Aug 95 21:58:44 +0100
References: <4120q0$9nj@hippo.shef.ac.uk>
Distribution: world
Organization: Max-Planck-Institut fuer Immunbiologie
Nntp-Posting-Host: mpi1.immunbio.mpg.de
X-News-Reader: VMS NEWS 1.24In-Reply-To: "D.J.Cant"'s message of 18 Aug 1995 12:24:32 GMTLines: 35
Lines: 35

In <4120q0$9nj@hippo.shef.ac.uk> "D.J.Cant" writes:

> I am looking for the addresses to the following institutes:
> 
> (If there is an Email address to contact them with, then so much the 
> better.)
> 
>          MRC (England)
>          ICRF (England)
>          EMBL (Germany)
>          Max Planck Inst. (Germany)
>          Biozentrum (Switzerland)
>          F. Miescher Inst. (Switzerland)
> 
> If as in some cases, there may be more than one address, i would likle to 
> have all of them.  I wouold be extremely appreciative of anyone who can 
> help me.  Please use my Email address to contact me.

Concerning "Max Planck Inst.": There are more than 60 Max-Planck-Institutes,
each dedicated to a specific topic. And the range is very broad (e.g.
"international and foreign law" or "immunobiology", both here in Freiburg).
Thus, you should be a bit more specific. Or are you really interested in
archaeology (this one is located in Rome and has not yet an email connection as
far as I know)?

Regards,
   Christoph Gartmann


+----------------------------------------------------------------------------+
| Max-Planck-Institut fuer      Phone   : +49-761-5108-465   Fax: -221       |
| Immunbiologie                 PSI     : PSI%(0262)45050160374::GARTMANN    |
| Postfach 1169                 Internet: gartmann@immunbio.mpg.de           |
| D-79011  Freiburg, FRG                                                     |
+----------- Do you know MENUE, the user environment for OpenVMS? -----------+

From owner-embldatabank@net.bio.net Mon Aug 21 23:00:00 1995
Newsgroups: bionet.molbio.embldatabank
Path: biosci!daresbury!hgmp.mrc.ac.uk!ebi.ac.uk!jecop
From: jecop@ebi.ac.uk (Jeroen Coppieters)
Subject: Re: Help!  I need some addresses! Email and Postal!
Sender: news@ebi.ac.uk (Mr news)
Message-ID: <DDpHx4.5ow@ebi.ac.uk>
Date: Tue, 22 Aug 1995 09:48:40 GMT
References: <4120q0$9nj@hippo.shef.ac.uk>
Organization: European Bioinformatics Institute
X-Newsreader: TIN [version 1.2 PL2]
Lines: 47

D.J.Cant (D.J.Cant@shef.ac.uk) wrote:
: I am looking for the addresses to the following institutes:

: (If there is an Email address to contact them with, then so much the 
: better.)

:          MRC (England)
As you are mailing from the UK, I would expect you know that "MRC" is not an 
institute. You can get information on MRC institutes at
http://scitsc.wlv.ac.uk/ukinfo/uk.map.res.html
:          ICRF (England)
:          EMBL (Germany)
You can find information on EMBL Heidelberg, and the 3 outstations (Hamburg,
Grenoble, Hinxton) at http://www.embl-heidelberg.de
Depending on whom you want to contact, I could give you several 100 email 
addresses.
:          Max Planck Inst. (Germany)
:          Biozentrum (Switzerland)
http://www.ch.embnet.org
:          F. Miescher Inst. (Switzerland)

: If as in some cases, there may be more than one address, i would likle to 
: have all of them.  I wouold be extremely appreciative of anyone who can 
: help me.  Please use my Email address to contact me.



:                  Charles Cant.



--
==============================================================
         . O .                               Jeroen Coppieters
     . O O o   O .                            Software Support
   O O O O *o    O O          Jeroen.Coppieters@embl-ebi.ac.uk
  O O O O(   *o  )O O                     (or jecop@ebi.ac.uk)
  )O O O O   o*  O O(                         ++44 1223 494422 
  O O O O( o*    )O O
  )O O O O  *o   O O(                      EMBL Outstation EBI
  O O O O(   *o  )O O      (European Bioinformatics Institute)
  )O @ O O   o*  O O(                             Hinxton Hall
    O O O( o*   )O('                                   Hinxton
     ` O(   *o O  '                         Cambridge CB10 1RQ
         ` O '                                              UK
http://www.ebi.ac.uk
==============================================================

From owner-embldatabank@net.bio.net Tue Aug 29 23:00:00 1995
Path: biosci!daresbury!bioftp.unibas.ch!citi2.fr!jussieu.fr!univ-lyon1.fr!swidir.switch.ch!swsbe6.switch.ch!scsing.switch.ch!news.rccn.net!iris02.itqb.unl.pt!doom.itqb.unl.pt!amartins
From: amartins@doom.itqb.unl.pt (Alexandra Martins)
Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.genbank,bionet.genome.chromosomes,bionet.molbio.embldatabank
Subject: Vero cells transfection/internal control
Date: 30 Aug 1995 18:35:45 GMT
Organization: Instituto Gulbenkian de Ciencia
Lines: 11
Message-ID: <422b21$ql3@iris02.itqb.unl.pt>
NNTP-Posting-Host: doom.itqb.unl.pt
Keywords: Transfection, monkey cells, transient expression
X-Newsreader: TIN [version 1.2 PL2]
Xref: biosci bionet.molbio.methds-reagnts:32857 bionet.molbio.genbank:2103 bionet.genome.chromosomes:792 bionet.molbio.embldatabank:538

Dear netters,

I am doing transient expression experiments using Vero cells (green
monkey kidney cells) and I need to probe the cellular DNA with an
endogeneous cellular gene.
Can anyone provide me the sequence of a Vero gene with a known copy
number?
Thanks in advance,

Alexandra Martins


