From owner-embldatabank@net.bio.net Mon Apr 01 23:00:00 1996
Path: biosci!agate!usenet.ins.cwru.edu!gatech!newsfeed.internetmci.com!uwm.edu!chi-news.cic.net!news.uoregon.edu!newsfeed.orst.edu!news.orst.edu!ava.bcc.orst.edu!fieldk
From: fieldk@ava.bcc.orst.edu (Katharine Field)
Newsgroups: bionet.molbio.embldatabank
Subject: Molecular Biology Workshop
Date: 1 Apr 1996 17:16:52 GMT
Organization: Oregon State University
Lines: 143
Message-ID: <4jp324$agn@news.orst.edu>
NNTP-Posting-Host: ava.bcc.orst.edu

                       
   Announcing a Workshop on Methods in Molecular Biology
              at Oregon State University 1996

Offered by the  Molecular and Cellular Biology Program 
Oregon State University
Corvallis, Oregon (541) 737-3799

An intensive training course for graduate students, faculty and researchers
in the biological and medical sciences

The methods of molecular biology, in particular recombinant DNA work, have
transformed research in the biological and medical sciences.  This
technology currently influences all aspects of biological research, has
far-reaching applications in clinical diagnosis, and has led to economically
important developments in agriculture and biotechnology.

This course provides a hands-on introduction to molecular
biological methods.  Participants will spend two weeks in intensive
laboratory work.  In addition, daily lectures by molecular biologists will
provide theoretical background and insight into cutting-edge research
developments and techniques.

Participants will learn the following techniques:
 cloning vector manipulation
 the polymerase chain reaction (PCR)
 preparation of recombinant DNA
 transformation of bacterial cells
 isolation of genomic DNA and RNA
 restriction analysis
 handling of radioactive isotopes
 isotopic and non-isotopic probe labelling
 Southern blots
 site-directed mutagenesis
 northern blots
 DNA sequencing
 synthesis of a fusion protein
 protein expression
 western blots

Prerequisites:  A good, recent background in DNA structure and chemistry,
genetics and biochemistry.

                             Schedule:  
MCB 525 - Methods in Molecular Biology
Session 1       July 15 - July 27, 1996
Session 2       September 9  - September 21,  1996

This is a highly intensive training course, requiring a full-time
committment from the participants.  Activities are scheduled all day and
some evenings.  Classes begin in the laboratory at 8:00 AM.  The lecture
portion of the course is at 1:00 PM.  There will be one Sunday off in the
middle of the session. 

Registration will be limited to 24 students per session.  A limited number
of places in the course will be reserved for MCB students and other OSU
graduate students who are required to take the course as part of their
programs.  All other course participants will be charged a workshop fee of
$750 to take the course, or $900 to take the course and receive 3 credits
through Continuing Higher Education (CHE).

                            Important!!! 
OSU graduate students who are required to take MCB 525 as part of their 
graduate program must provide copies of their official programs, or a 
letter from their major professors, along with the application, in order 
to be considered for the course. 

                      Registration, Fees & Credit
Please fill out and return by April 15, 1996.  You will be notified if you
have been accepted and registration forms will be mailed to you.  You do not
need to send in an application if you will be taking the lecture course only.
Workshop fees must be paid in full by two weeks before the first day of class. 

                           Lectures only:
MCB 524 - is offered as a 1-credit, pass/no pass course through the summer
school (July) or as a fall-term course (September).  There is no limit on enrollment.  The course consists of approximately 15 one-hour lectures. MCB 525 students may not take  MCB 524.

                              Staff:
Dr. Katharine Field, Coordinator
Dr. Walt Ream, co-coordinator
Dr. Gary Merrill, co-coordinator
Faculty of the Molecular and Cellular Biology Program

                             Housing:
Housing in studio "quad" apartments (single rooms with shared kitchen and
bath) is available close to campus at a cost of $185 for two weeks.
Restaurants are a short distance from class.  A campus parking permit can be
purchased for $5 per week.

Class participants can use Dixon Recreation Center (lockers, swimming,
weight room, exercise equipment, outdoor track, and more) for $3 per day.

                            Location:
The class will be held in the new Agricultural and Life Sciences Building on
the Oregon State University campus in Corvallis.  Corvallis is a community
of 45,000 residents located in the beautiful Willamette River Valley, an
hour from the Pacific Coast and the Cascade Range.  

                               APPLICATION:
------------------------------------------------------------------------------
Name:   

Position:       

Organization or School, P. I.'s name:    

Highest Degree, Date:    

Address: 

City:                         State:        Zip:      

Phone: (work)               (home)   

email address:   

Preferred Session:  _____July 15-July 27, 1996
               	    _____September 9 - September 21, 1996

Status:  _____ Graduate Course for Credit/Program
         _____ Workshop Course - no credit ($750)
         _____ Workshop Course - 3 credits from CHE ($900)

If you do not get into your preferred session, do you want to be considered
for the other session? _____ Yes _____ No      
I will need housing    _____ Yes _____ No

Return to: MCB 525 Application
           Molecular and Cellular Biology
           3021 Agricultural and Life Sciences
           Oregon State University
           Corvallis, OR  97331-7303     
Or send via email to mcb@bcc.orst.edu
Further Questions: Please call (541) 737-3799 or email  mcb@bcc.orst.edu

Oregon State University is an Affirmative Action/Equal Opportunity employer
and complies with Section 504 of the Rehabilitation Act of 1973.  







From owner-embldatabank@net.bio.net Tue Apr 02 23:00:00 1996
Path: biosci!daresbury!nntp-trd.UNINETT.no!newsfeed.sunet.se!news01.sunet.se!sunic!news99.sunet.se!news.funet.fi!news.csc.fi!nokia.fi!news.nmp.nokia.com!ourf14.nmp.nokia.com!jpn
From: jpn@ourf14.nmp.nokia.com (Jukka Polonen)
Newsgroups: bionet.molbio.embldatabank
Subject: q
Date: 2 Apr 1996 12:43:00 GMT
Organization: Nokia Mobile Phones
Lines: 1
Message-ID: <4jr7ck$j6t@sanfs04.nmp.nokia.com>
NNTP-Posting-Host: ourf14.nmp.nokia.com
X-Newsreader: TIN [version 1.2 PL2]



From owner-embldatabank@net.bio.net Tue Apr 02 23:00:00 1996
Newsgroups: bionet.molbio.embldatabank
Path: biosci!daresbury!hgmp.mrc.ac.uk!ebi.ac.uk!EMBL-EBI.ac.uk!malik
From: malik@EMBL-EBI.ac.uk (Ansar Malik)
Subject: ANNOUNCEMENT: Immunogenetics IMGT database - update 
Sender: news@ebi.ac.uk (Mr news)
Message-ID: <DpA8rq.Bvz@ebi.ac.uk>
Date: Wed, 3 Apr 1996 10:50:13 GMT
Lines: 56
Reply-To: malik@EMBL-EBI.ac.uk (Ansar Malik)
Organization: European Bioinformatics Institute (EMBL) - UK
X-Newsreader: mxrn 6.18-32
Keywords: database, immunology, T-cell-receptor, sequences, immunogenetics.


IMMUNOGENETICS IMGT DATABASE
===================================

Professor Marie-Paule LEFRANC
Laboratoire d'ImmunoGenetique Moleculaire, LIGM
UMR CNRS 9942, Institut de Genetique Moleculaire
BP 5051, 1919 route de Mende, 34033 MONTPELLIER Cedex 1
FRANCE
Telephone: (33) 67 61 36 34
FAX      : (33) 67 04 02 31/67 04 02 45
e-mail   : lefranc@ligm.crbm.cnrs-mop.fr

INTRODUCTION
============

The ImMunoGeneTics database, IMGT, is an integrated specialised database
containing nucleotide sequence information of genes important in the function
of the immune system. It collects and annotates sequences belonging to the
immunoglobulin superfamily which are involved in immune recognition, these are
the B cell antigen receptor (Immunoglobulin or Ig), the T cell antigen receptor
(TCR) (LIGM-database) and the class I and class II molecules of the 
Human Leucocyte Antigens (HLA) system (HLA-database).

IMGT/LIGM DATABASE  
==================
An integrated immumogenetics database (IMGT/LIGM) specialising in Ig and TcR is
under development through collaboration between LIGM, IFG and EMBL oustation
EBI. This database consists of the Ig and TcR sequence entries.
Collaborators:
  
Release 3 (March 1996) contains 5109 fully annotated sequence entries.
 
LIGM Montpellier :Marie-Paule Lefranc (coordinator)
                  Veronique Giudicelli,Denys Chaume
EMBL-EBI         :Ansar Malik
IFG              :Werner Mueller
MRC 	         :Ian Tomlinson

ACCESS/DATA DISTRIBUTION
========================

WWW server:     http://www.ebi.ac.uk/imgt/
FTP server:     ftp.ebi.ac.uk/pub/databases/imgt
E_MAIL server:  email netserv@ebi.ac.uk  -- send "help IMGT" in the mail body.

This database is available on CD-ROM as an acompanying database
to the EMBL Nucleotide Sequence Database at nominal cost.

=======================================================================
Ansar Malik Ph.D                        | Email:Malik@ebi.ac.uk
EBI - European Bioinformatics Institute | URL:  http://www.ebi.ac.uk
Hinxton Hall, Hinxton                   | Tel:  +44 (1223) 494417
Cambridge CB10 1RQ, UK                  | Fax:  +44 (1223) 494968
========================================================================


From owner-embldatabank@net.bio.net Fri Apr 05 23:00:00 1996
Path: biosci!biosci!not-for-mail
From: apweiler@ebi.ac.uk (Rolf Apweiler)
Newsgroups: ebi.servers,bionet.general,bionet.molbio.embldatabank,bionet.molbio.proteins,embnet.general
Subject: ANNOUNCEMENT: Beta release of TREMBL, a supplement to SWISS-PROT
Date: 6 Apr 1996 15:53:05 -0800
Organization: European Bioinformatics Institute (EMBL) - UK
Lines: 81
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <DpE8y8.H1G@ebi.ac.uk>
Reply-To: apweiler@ebi.ac.uk (Rolf Apweiler)
NNTP-Posting-Host: net.bio.net
Xref: biosci bionet.general:20998 bionet.molbio.embldatabank:624 bionet.molbio.proteins:7526


The Beta release of TREMBL, a protein sequence database supplementing the 
SWISS-PROT Protein Sequence Data Bank is now available from  
ftp.ebi.ac.uk in directory /pub/databases/trembl.


INTRODUCTION
============

TREMBL is a protein sequence database supplementing the SWISS-PROT Protein
Sequence Data Bank. TREMBL contains the translations of all coding 
sequences (CDS) present in the EMBL Nucleotide Sequence Database not 
integrated in SWISS-PROT. At the moment we treat TREMBL as an independent 
dataset in SWISS-PROT format, but shortly TREMBL will become a part of 
SWISS-PROT. 


BETA RELEASE OF TREMBL
======================

This TREMBL release is created from the EMBL nucleotide sequence release 46
and contains 98'447 sequence entries, comprising 25'879'831 amino acids.

TREMBL is split in two main sections; SP-TREMBL and REM-TREMBL:

SP-TREMBL (SWISS-PROT TREMBL) contains the entries (81'861) which should be 
incorporated into SWISS-PROT. SP-TREMBL is partially redundant against 
SWISS-PROT, since approximately 40'000 SP-TREMBL entries are only 
additional sequence reports of proteins already in SWISS-PROT. We will try 
to merge these sequence reports as fast as possible with the already 
existing SWISS-PROT entries for these proteins, so as to make SWISS-PROT
and TREMBL completely nonredundant.
SP-TREMBL is organized in subsections:

fun.dat (Fungi):                3772 entries
inv.dat (Invertebrates):        9689 entries
mam.dat (Other Mammals):        1907 entries
mhc.dat (MHC proteins):         2007 entries
org.dat (Organelles):           5241 entries
phg.dat (Bacteriophages):        939 entries
Pln.dat (Plants):               5294 entries
pri.dat (Primates):             6425 entries
pro.dat (Prokaryotes):         15645 entries
rod.dat (Rodents):              5837 entries
unc.dat (Unclassified):          201 entries
vrl.dat (Viruses):             22677 entries
vrt.dat (Other Vertebrates):    2227 entries


REM-TREMBL (REMaining TREMBL) contains the entries (16'586) that we do 
not want to include in SWISS-PROT.

ACCESS/DATA DISTRIBUTION
========================

FTP server:     ftp.ebi.ac.uk/pub/databases/trembl

TREMBL is also available on the SWISS-PROT CD-ROM.



TREMBL HAS BEEN PREPARED BY:
============================

Rolf Apweiler, Alain Gateau, Vivien Junker, Fiona Lang, and Claire 
O'Donovan at the EMBL Outstation - European Bioinformatics Institute
(EBI) in Hinxton;
Amos Bairoch at the Medical Biochemistry Department of the University 
of Geneva.

=======================================================================
Rolf Apweiler                           | Email:apweiler@ebi.ac.uk
EBI - European Bioinformatics Institute | URL:  http://www.ebi.ac.uk
Wellcome Trust Genome Campus, Hinxton   | Tel:  +44 (1223) 494435
Cambridge CB10 1RQ, UK                  | Fax:  +44 (1223) 494968
========================================================================






From owner-embldatabank@net.bio.net Mon Apr 08 23:00:00 1996
Path: biosci!uunet.uu.net!aditya!raman
From: aditya!raman@uunet.uu.net (K. Ramnarayan)
Newsgroups: bionet.molbio.embldatabank
Subject: (none)
Date: 9 Apr 1996 13:22:40 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 1
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199604031649.IAA05464@aditya.tbc.com>
NNTP-Posting-Host: net.bio.net

unsubscribe embl-db ramnarayan

From owner-embldatabank@net.bio.net Mon Apr 08 23:00:00 1996
Path: biosci!daresbury!usenet
From: Martin Hilbers <mph@dl.ac.uk>
Newsgroups: bionet.molbio.embldatabank,embnet.general
Subject: Re: ANNOUNCEMENT: Beta release of TREMBL, a supplement to SWISS-PROT
Date: 9 Apr 1996 10:12:38 GMT
Organization: Daresbury Lab, Warrington, U.K.
Lines: 18
Distribution: bionet
Message-ID: <4kdd6m$kfs@mserv1.dl.ac.uk>
References: <DpE8y8.H1G@ebi.ac.uk>
NNTP-Posting-Host: s-ind2.dl.ac.uk
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 1.1IS (X11; I; IRIX 5.3 IP12)
X-URL: news:DpE8y8.H1G@ebi.ac.uk

Wow,

Looks nice. But what is the relation between this TREMBL and the TREMBL
produced by Thure Etzold in Heidelberg, which was also created from
EMBL 46.0 and contains 169636 entries ?


Martin
-- 
---------------------------------------------------------------------------
|  Martin Hilbers          |       E-mail: m.p.hilbers@dl.ac.uk           |
|  SEQNET                  |       Tel:    +44-1925-603492                |
|  Daresbury Laboratory    |       Fax:    +44-1925-603100                |
|  Daresbury, Warrington   |----------------------------------------------|
|  Cheshire WA4 4AD        |    SEQNET is the UK national EMBNet node     |
|  United Kingdom          |    http://www.dl.ac.uk/SEQNET/home.html      |
---------------------------------------------------------------------------


From owner-embldatabank@net.bio.net Mon Apr 08 23:00:00 1996
Path: biosci!biosci!not-for-mail
From: Matteo diTommaso <ditommaso@ebi.ac.uk>
Newsgroups: hinxton.announce,embnet.general,ebi.general,bionet.software,bionet.molbio.embldatabank
Subject: "SynCron" - Tools for EMBL Database Update
Date: 8 Apr 1996 21:39:21 -0700
Organization: European Bioinformatics Institute
Lines: 72
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <NEWTNews.828977543.8674.tommaso@juliet.ebi.ac.uk>
NNTP-Posting-Host: net.bio.net
Xref: biosci bionet.software:15195 bionet.molbio.embldatabank:625


Subj="SynCron" tools for maintaining synchronised copies of the EMBL 
Nucleotide Sequence Database  

Looking at the statistics for access to the EMBL Nucleotide Sequence Database 
update files on the EBI ftp site (ftp://ftp.ebi.ac.uk/pub/databases/embl/new/) 
we observe that many people download the full cumulative data file 
(cumulative.dat) rather than the daily update files.  In an attempt to make 
daily update files more useful and to provide a reliable mechanism for 
re-generating the cumulative.dat file locally from daily updates, the EBI and 
the Swiss EMBNet node have jointly developed a set of tools which can be used 
to fetch the daily updates and update the local database.

The programs make use of 'transaction listings' made available on the EBI ftp 
site. These transaction listings are now supplied with every update file and 
include a record of each update, insert and delete operation to the EMBL 
Nucleotide Sequence Database as represented in the flat-file updates.  The 
naming scheme for transaction listings is the same as for daily, weekly, and 
cumulative updates with the extension ".lis".  The transaction listings are 
found in:

ftp://ftp.ebi.ac.uk/pub/databases/embl/new/list/

and look like:

Acc#   ID  Action DateStamp  Ver# Division
T58328 AA328 U 19951108232958 3    EST
T58329 AA329 C 19951108233007 3    EST
T58330 AA330 D 19951108233015 3    EST
R67977 AA977 U 19951108230600 3    EST
R67978 AA978 U 19951108230611 3    EST 
where U=Update C=Create D=Delete

Using the tools it is possible to regenerate the cumulative.dat file (at a 
remote site) reliably from daily updates.  Validation of the new 
cumulative.dat file is also possible using the transaction listing provided at 
the EBI.

Using these programs it should be possible to keep a copy of the EMBL 
Nucleotide Sequence Database that exactly matches the contents of the database 
in operation at the EBI for external services with manual intervention 
required only in the event of some failure in network transfer of the file - 
etc.

These programs are available by anonymous ftp from (the _002 version number 
will change as the programs are updated):

UNIX Version:
ftp://ftp.ebi.ac.uk/pub/software/unix/listtools/SynCron_002.tar.gz

VMS Version:
(backup/gzip)
ftp://ftp.ebi.ac.uk/pub/software/vms/listtools/SynCron_002.bck-gz
OR
(tar/compress)
ftp://ftp.ebi.ac.uk/pub/software/vms/listtools/SynCron_002.tar_Z


Matteo diTommaso
Database Programming Group
EMBL Outstation
The European Bioinformatics Institute
E-mail:   ditommaso@ebi.ac.uk  


Nicole Redaschi and Reinhard Doelz
Biozentrum - University of Basel
EMBnet Node Switzerland  
E-Mail: embnet@comp.bioz.unibas.ch




From owner-embldatabank@net.bio.net Wed Apr 10 23:00:00 1996
Newsgroups: bionet.molbio.embldatabank,embnet.general
Path: biosci!daresbury!hgmp.mrc.ac.uk!ebi.ac.uk!EMBL-EBI.ac.uk!apweiler
From: apweiler@EMBL-EBI.ac.uk (Rolf Apweiler)
Subject: Re: ANNOUNCEMENT: Beta release of TREMBL, a supplement to SWISS-PROT
Sender: news@ebi.ac.uk (Mr news)
Message-ID: <Dpp4qy.Dz6@ebi.ac.uk>
Date: Thu, 11 Apr 1996 11:47:22 GMT
Distribution: bionet
Lines: 122
Reply-To: apweiler@EMBL-EBI.ac.uk (Rolf Apweiler)
References: <DpE8y8.H1G@ebi.ac.uk> <4kdd6m$kfs@mserv1.dl.ac.uk>
Organization: European Bioinformatics Institute (EMBL) - UK
X-Newsreader: mxrn 6.18-32


In article <4kdd6m$kfs@mserv1.dl.ac.uk>, Martin Hilbers <mph@dl.ac.uk> writes:
>Wow,
>
>Looks nice. But what is the relation between this TREMBL and the TREMBL
>produced by Thure Etzold in Heidelberg, which was also created from
>EMBL 46.0 and contains 169636 entries ?
>
>
>Martin

Hi Martin,

Thure Etzolds TREMBL and TREMBL done by SWISS-PROT are related. TREMBL done 
by SWISS-PROT is the successor or the next generation of Thures TREMBL.  
SWISS-PROT kept the name TREMBL for their Translation of EMBL CDS, since 
the tools used to create the translations of the CDS are based on the 
program 'trembl' written by Thure Etzold at the EMBL in Heidelberg.

So, what are the differences?
Thure translates all CDS in the EMBL nucleotide sequence database into 
TREMBL entries. 
TREMBL done by SWISS-PROT excludes all the entries which are already 
integrated in SWISS-PROT to make SWISS-PROT and TREMBL non-redundant.
Also, all TREMBL entries are in SWISS-PROT format
and SWISS-PROT merges TREMBL entries and adds computer generated 
annotation, references and cross-references to other databases.
So, the entries are quite different. An example see below.

In June the first official release of TREMBL done by SWISS-PROT will be 
available and at that stage Thures TREMBL will disappear. However, everybody 
who wants to generate a translation of all EMBL CDS as single entries can 
still use Thures program 'trembl' to do so; it will be distributed as 
part of SRS.

For more information see the release notes of TREMBL and SWISS-PROT or 
read:
              Bairoch A., and Apweiler R.
              The SWISS-PROT protein sequence data bank and its new
              supplement TREMBL.
              Nucleic Acids Res. 24:21-25(1996).


We hope this info helps.


Rolf Apweiler and Thure Etzold


Examples

Thures TREMBL entry:

ID   S64977_2  standard; PRT; 228 AA.
AC   S64977;
DR   EMBL; S64977; S64977.
DE   gene: "COII"; 
DE   COI=cytochrome oxidase subunit I...COIII=cytochrome oxidase subunit
DE   III [Drosophila simulans, Mitochondrial, 8 genes, 1990 nt]
OS   Drosophila simulans (fruit fly)
OC   Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
OC   Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
OC   Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
OG   Mitochondrion
FT   CDS             115..799
FT                   /note="Description: cytochrome oxidase subunit II"
FT                   /gene="COII"
FT                   /db_xref="FLYBASE:FBgn0012877"
FT                   /db_xref="PID:e87967"
FT                   /translation="MSTWANLGLQDSASPLMEQLIFFHDHALLILVMITVLVGYLMFML
FT                   FFNNYVNRFLLHGQLIEMIWTILPAIILLFIALPSLRLLYLLDEINEPSVTLKSIGHQW
FT                   YWSYEYSDFNNIEFDSYMIPTNELSIDGFRLLDVDNRVILPMNSQIRILVTAADVIHSW
FT                   TIPALGVKVDGTPGRLNQTNFFINRPGLFYGQCSEICGANHSFMPIVIESVPVNHFIKW
FT                   ISSNNS"
CC   translated using genetic code table "Invertebrate Mitochondrial"
SQ   Sequence   228 BP;
>S64977_2
MSTWANLGLQDSASPLMEQLIFFHDHALLILVMITVLVGYLMFMLFFNNYVNRFLLHGQL
IEMIWTILPAIILLFIALPSLRLLYLLDEINEPSVTLKSIGHQWYWSYEYSDFNNIEFDS
YMIPTNELSIDGFRLLDVDNRVILPMNSQIRILVTAADVIHSWTIPALGVKVDGTPGRLN
QTNFFINRPGLFYGQCSEICGANHSFMPIVIESVPVNHFIKWISSNNS


Corresponding SWISS-PROT TREMBL entry:

ID   E87967         PRELIMINARY;   PRT;   228 AA.
DT   26-MAR-1996 (EMBLREL. 46, CREATED)
DT   26-MAR-1996 (EMBLREL. 46, LAST SEQUENCE UPDATE)
DT   26-MAR-1996 (EMBLREL. 46, LAST ANNOTATION UPDATE)
DE   CYTOCHROME C OXIDASE POLYPEPTIDE II (EC 1.9.3.1).
GN   COII.
OS   DROSOPHILA SIMULANS (FRUIT FLY).
OG   MITOCHONDRION.
OC   EUKARYOTA; METAZOA; ARTHROPODA; INSECTA; DIPTERA.
RN   [1]
RP   SEQUENCE FROM N.A.
RX   MEDLINE; 93374296.
RA   KANEKO M., SATTA Y., MATSUURA E.T., CHIGUSA S.I.;
RL   GENET. RES. 61:195-204(1993).
CC   -!- CATALYTIC ACTIVITY: 4 FERROCYTOCHROME C + O(2) = 2 H(2)O + 4
CC       FERRICYTOCHROME C.
CC   -!- FUNCTION: SUBUNIT I AND II FORM THE FUNCTIONAL CORE OF THE ENZYME
CC       COMPLEX. ELECTRONS ORIGINATING IN CYTOCHROME C ARE TRANSFERRED VIA
CC       HEME A AND CU(A) TO THE BINUCLEAR CENTER FORMED BY HEME A3 AND
CC       CU(B). SUBUNIT II BINDS CU(A) AND CYTOCHROME C.
CC   -!- COFACTOR: COPPER A AND HEME GROUP.
CC   -!- SUBCELLULAR LOCATION: INTEGRAL MEMBRANE PROTEIN. (MITOCHONDRIAL).
CC   -!- SIMILARITY: TO OTHER MITOCHONDRIAL OR BACTERIAL COX2 SUBUNITS.
DR   EMBL; S64977; E87967; -.
DR   FLYBASE; FBGN0012877; DSIM\MT:COI.
DR   PROSITE; PS00078; COX2.
KW   MITOCHONDRION; OXIDOREDUCTASE; COPPER; TRANSMEMBRANE.
FT   METAL       196    196       COPPER A (PROBABLE).
FT   METAL       200    200       COPPER A (PROBABLE).
FT   METAL       204   >204       COPPER A (PROBABLE).
SQ   SEQUENCE   228 AA;  26160 MW;  0E17E9D6 CRC32;
     MSTWANLGLQ DSASPLMEQL IFFHDHALLI LVMITVLVGY LMFMLFFNNY VNRFLLHGQL
     IEMIWTILPA IILLFIALPS LRLLYLLDEI NEPSVTLKSI GHQWYWSYEY SDFNNIEFDS
     YMIPTNELSI DGFRLLDVDN RVILPMNSQI RILVTAADVI HSWTIPALGV KVDGTPGRLN
     QTNFFINRPG LFYGQCSEIC GANHSFMPIV IESVPVNHFI KWISSNNS
//


From owner-embldatabank@net.bio.net Sun Apr 28 23:00:00 1996
Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!news2.digex.net!usenet
From: Vadim Babenko <74672.2423@compuserve.com>
Newsgroups: bionet.molbio.embldatabank
Subject: MAC Version of FREE Vector NTI Viewer
Date: 29 Apr 1996 17:41:06 GMT
Organization: Express Access Online Communications, USA
Lines: 13
Message-ID: <4m2uvi$8bj@news4.digex.net>
NNTP-Posting-Host: informax.digex.net

InforMax, Inc., a provider of molecular biology software and general software solutions,
has released the MAC version of its FREE Vector NTI Viewer which can be downloaded 
from http://www.informaxinc.com. The Viewer can be installed as a helper application 
within WWW browsers, such as Netscape or Microsoft Internet Explorer, to retrieve and 
visualize molecule data in Vector NTI Molecule Document, GenBank, EMBL and 
FASTA formats. It also makes it possible to visualize structures stored as MIME 
attachments in Internet mail messages. The Viewer can read GenBank, EMBL, FASTA 
files and unannotated nucleotide sequences from ASCII files and create and visualize 
virtual molecules for them. The Viewer allows simple molecule analysis - searching for 
restriction sites, ORFs, etc. Publication-quality graphics maps may be printed, saved in an 
external file, or transferred via the clipboard to other applications quickly and easily for 
inclusion in articles and reports. 


