From owner-embldatabank@net.bio.net Tue Nov 05 22:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.embldatabank Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 6 Nov 1996 02:03:33 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199611061000.CAA02527@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-embldatabank@net.bio.net Thu Nov 07 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: scistra@cs.sandia.gov (Sorin C. Istrail) Newsgroups: bionet.molbio.embldatabank Subject: RECOMB97: Hotel Reservation and Registration Information Date: 8 Nov 1996 01:55:48 -0000 Lines: 114 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <55u3v4$1nd@mserv1.dl.ac.uk> Original-To: embl-db@dl.ac.uk Please call the ElDorado hotel and make your reservation AS SOON AS POSSIBLE. This is a four-star and four-diamond hotel and we have a limited number of rooms avaiblable. ElDorado will direct the overflow to another hotel. FIRST ANNUAL INTERNATIONAL CONFERENCE ON COMPUTATIONAL MOLECULAR BIOLOGY RECOMB 97 http://www.cs.sandia.gov/recomb97 January 20-23, 1997 Eldorado Hotel Santa Fe, New Mexico Sponsored by SIGACT with support from SLOAN Foundation US Department of Energy ELDORADO HOTEL RESERVATIONS INFORMATION **** A four-star, four diamond-hotel **** Phones: 1-800 955-4455 505-988-4455 Fax: 505-995-4544 (reservations only) Webpage: http://www.eldoradohotel.com Email: rez@eldoradohotel.com Room Rates: Single/double $85 Sunday(1/19), Monday(1/20), Tuesday(1/21), Wednesday(1/22), Thursday(1/23), Friday(1/24) Saturday (1/25) Single/Double $135 Saturday(1/18) (For comparison, if reservations are not made at the RECOMB97 group rate, the average single/double rate would be $144 for these types of rooms.) RECOMB97 REGISTRATION INFORMATION A registration form can be obtained from the conference web page http://www.cs.sandia.gov/recomb97 Advanced Registration ACM Member Non-ACM Member $285 $360 Late/On-site Registration (After December 12) ACM Member Non-ACM Member $360 $435 Full time student Advanced Registration $160 Late/On-site Registration (After Decemebr 12) $210 Registration fee includes: one copy of the proceedings, four lunches, eight coffee breaks, and a banquet ticket. Student registration fee includes all of the above except the banquet ticket. For more information check out the conference web page http://www.cs.sandia.gov/recomb97 or contact Sorin Istrail RECOMB97 Conference Chair Sandia National Laboratories Massively Parallel Computing Research Laboratory MS 1110 Albuquerque, NM 87185-1110 Phone: (505) 845-7612 Fax : (505) 845-7442 Secretary: (505) 845-7432 Email: scistra@cs.sandia.gov www : http://www.cs.sandia.gov/~scistra From owner-embldatabank@net.bio.net Tue Nov 12 22:00:00 1996 Path: biosci!rutgers!uwm.edu!www.nntp.primenet.com!nntp.primenet.com!EU.net!news.sprintlink.net!news-peer.sprintlink.net!uunet!in3.uu.net!news.u.washington.edu!saul1.u.washington.edu!egn From: "E. Kolker" Newsgroups: bionet.molbio.embldatabank Subject: Abstract Date: Tue, 12 Nov 1996 16:01:31 -0800 Organization: University of Washington Lines: 83 Message-ID: References: <55u3v4$1nd@mserv1.dl.ac.uk> NNTP-Posting-Host: saul1.u.washington.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII NNTP-Posting-User: egn In-Reply-To: <55u3v4$1nd@mserv1.dl.ac.uk> ANNOUNCEMENT AND CALL FOR PAPERS Computational Biology Session: "Computing in the Genome Era" Eleventh International Conference on Mathematical and Computer Modelling and Scientific Computing March 31 - April 3, 1997 Georgetown University Conference Center Washington, DC, USA The Eleventh International Conference on Mathematical and Computer Modelling and Scientific Computing is scheduled to take place March 31 - April 3, 1997 at the Georgetown University Conference Center, Washington, DC, U.S.A. Plenary lectures by world-renowned scientists and sessions on many recent developments in engineering and sciences comprise a long-standing tradition at the ICMCM's. Mathematical and computer modelling and scientific computing have become powerful tools for solving complex problems and providing greater insights into the future. The objective of the conference is to bring together researchers from various disciplines including the traditional and emerging areas of engineering and sciences for cross-fertilization of ideas and exchange of information. The objective of the Computational Biology Session "Computing in the Genome Era" is to discuss the current state of computational biology, its approaches, methods, general problems, achievements, and future developments with emphasis on sequence research and analysis for the Genome Projects. Speakers of the session include: S. Altschul (NCBI, NIH, Bethesda), A. Bairoch (Geneva Univ., Switzerland), W. Gish (Wash. Univ., St. Louis), P. Green (Univ. of Wash., Seattle), S. Henikoff (FHCRC, HHMC, Seattle), L. Hood (Univ. of Wash., Seattle), E. Koonin (NCBI, NIH, Bethesda), D. Searls (SmithKline Beecham, King of Prussia), E. Trifonov (Weizmann Inst., Israel). Papers (Abstracts) are invited on all relevant aspects of computational biology for presentation at the session, to be selected on competitive basis by a steering committee. One-page abstracts (about 200 words) should clearly describe the work and its conclusions. Full length manuscripts (limited to six pages) of papers presented at the conference will be published in the Conference Proceedings, in a special issue of the journal MATHEMATICAL MODELLING AND SCIENTIFIC COMPUTING, Vol. 8, 1997 (ISSN 1067-0688). The manuscripts for the special issue are due June 15, 1997. The special issue of the journal will be published by September 1997. All participants shall pay the registration fee (pre-registration before February 15, 1997: IAMCM or IMACS members - $330, non-members - $375, graduate students - $210, undergraduate students - $175). Abstracts may be submitted in hard copy or via fax or by e-mail (preferred!) under subject "Abstract". The abstracts must be formatted to fit on 8-1/2 x 11 inch (approximately 21.5 cm x 28 cm or European Standard A-4 size) paper, typed in single space. The title must be capitalized and centered followed by the author's name(s), institution, and full address, including fax and e-mail. Send two copies (ONE copy if sent by fax or e-mail) of the abstract to the session organizer BEFORE November 30, 1996: Eugene Kolker Dept of Molecular Biotechnology and Genome Center Box 357730, University of Washington Seattle, WA 98195-7730, USA Fax: +1-206-685-7301 E-mail: egn@u.washington.edu Important dates: Closing date for receiving abstracts: November 30, 1996 Notification of acceptance: December 27, 1996 The Computational Biology Session is sponsored by SILICON GRAPHICS. Companies are welcome to participate in the session, present their tools and products, and/or co-sponsor the session. The Web site for the session is under contruction and will include information on the session, a list of related sites, links to the sponsors etc. From owner-embldatabank@net.bio.net Tue Nov 12 22:00:00 1996 Newsgroups: bionet.molbio.embldatabank Path: biosci!rutgers!uwm.edu!www.nntp.primenet.com!nntp.primenet.com!news.bbnplanet.com!cpk-news-hub1.bbnplanet.com!newsfeed.internetmci.com!malgudi.oar.net!utnetw!mabrini From: mabrini@uoft03.utoledo.edu (Pan Parag) Subject: Look here! Message-ID: Sender: news@utnetw.utoledo.edu (News Manager) X-Newsreader: TIN [version 1.2 PL2] Date: Tue, 12 Nov 1996 22:36:05 GMT Lines: 15 I am looking for anybody who is a practicing biologist or a biology research professional who can grant me a 15 minute interview preferably over the phone. I am doing this as part of my assignment for my Biology class so that I can graduate. If anybody is willing to help me out, please respond with your name and phone number to the following e-mail address: mabrini@uoft03.utoledo.edu. If my paper is successful, I will send you a copy of it in the mail. Mark Abrinica University of Toledo 419-472-2999 From owner-embldatabank@net.bio.net Wed Nov 13 22:00:00 1996 Path: biosci!rutgers!uwm.edu!www.nntp.primenet.com!nntp.primenet.com!howland.erols.net!panix!news.columbia.edu!news From: Richard Friedman Newsgroups: bionet.molbio.embldatabank Subject: Re: Look here! Date: Thu, 14 Nov 1996 09:19:44 +0100 Organization: Columbia University Lines: 1 Message-ID: <328AD620.5808@convex.hhmi.columbia.edu> References: NNTP-Posting-Host: ghostwriter.ccc.columbia.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01 (Macintosh; I; PPC) To: Pan Parag ok, I'm at (212)305-1667 -Rich From owner-embldatabank@net.bio.net Sat Nov 16 22:00:00 1996 Path: biosci!ihnp4.ucsd.edu!agate!howland.erols.net!bloom-beacon.mit.edu!senator-bedfellow.mit.edu!usenet From: Bugra Giritlioglu Newsgroups: bionet.molbio.bio-matrix,bionet.molbio.embldatabank,bionet.molbio.evolution Subject: CAN TWO MEN (or women) HAVE KIDS??? Date: Sat, 16 Nov 1996 19:37:35 -0500 Organization: Massachvsetts Institvte of Technology Lines: 21 Message-ID: <328E5E4F.DFC@mit.edu> NNTP-Posting-Host: modese.mit.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0Gold (Win95; I) Xref: biosci bionet.molbio.bio-matrix:790 bionet.molbio.embldatabank:711 bionet.molbio.evolution:5329 I am curious to see what people know about the possibility of same-sex parenting, i.e. the possibility of achieving fertilization by combining the genetic material from two sperms (or two eggs). I recently corresponded with an expert on this topic, and to my surprise, discovered that it may not be as impossible and crazy as it sounds. (hi again, if you subscribe to any of these newsgroups!!) I am trying to get in touch with practically anybody who is excited about this possibility and knows more about it than I do. My knowledge of the biology of reproduction is very hazy, however, I would seriously consider going back to school in molecular genetics if I knew that there were others interested in making this a reality. Is anybody out there doing research that might, directly or indirectly, benefit this cause. Give me a holler!! Please help me get started if you have any ideas, suggestions, etc. Also, any suggestions as to where else I might try posting this message? From owner-embldatabank@net.bio.net Sat Nov 16 22:00:00 1996 Path: biosci!agate!howland.erols.net!news.bbnplanet.com!cam-news-hub1.bbnplanet.com!uunet!in2.uu.net!newsgate.compuserve.com!news.production.compuserve.com!news From: dr. bev <74731.463@CompuServe.COM> Newsgroups: bionet.molbio.embldatabank,bionet.molbio.methds-reagnts Subject: oligonucleotide probe for bikunin Date: 10 Nov 1996 17:36:45 GMT Organization: self Lines: 10 Message-ID: <5653rd$m0e$1@mhafc.production.compuserve.com> Xref: biosci bionet.molbio.embldatabank:714 bionet.molbio.methds-reagnts:51595 I am a researcher interested in finding out whether an oligonucleotide probe for rat bikunin has ever been described in the biomedical literature. I have done online searches in medline and in genebanks on line (embldatabank and gdb). I already found oligonucleotide probes for human and mouse bikunin, but I need an oligonucleotide probe for the rat. Can anyone give me some tips on where else to look? Thanks. B. Waxler 74731.463@compuserve.com From owner-embldatabank@net.bio.net Sun Nov 17 22:00:00 1996 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.mel.connect.com.au!news.uwa.edu.au!newsman.murdoch.edu.au!vetmac3.murdoch.edu.au!user From: cummins@central.murdoch.edu.au (Jim Cummins) Newsgroups: bionet.molbio.bio-matrix,bionet.molbio.embldatabank,bionet.molbio.evolution Subject: Re: CAN TWO MEN (or women) HAVE KIDS??? Date: Mon, 18 Nov 1996 14:45:24 +0800 Organization: Murdoch University Lines: 19 Message-ID: References: <328E5E4F.DFC@mit.edu> NNTP-Posting-Host: vetmac3.murdoch.edu.au Xref: biosci bionet.molbio.bio-matrix:793 bionet.molbio.embldatabank:715 bionet.molbio.evolution:5336 In article <328E5E4F.DFC@mit.edu>, Bugra Giritlioglu wrote: > I am curious to see what people know about the possibility of same-sex > parenting, i.e. the possibility of achieving fertilization by combining > the genetic material from two sperms (or two eggs). This probably would not work as differential methylation during genomic imprinting in the gonad treats the male genes differently from the female. Male genes acting in the embryo serve to maximise growth and placental function at the expense of the maternal organism. Female genes serve to mimimise growth and preserve the maternal interest. One would have to de-imprint the genes to have same-sex fertilization. Occasionally one gets a dispermic egg developing without the egg's genes participating - the end result if a hydatidiform mole - a monster. Ocasionally eggs will activate spontaneously in the ovary and result in an ovarian teratoma. So we DO need two genders! -- URL http://numbat.murdoch.edu.au/spermatology/spermhp.html From owner-embldatabank@net.bio.net Sun Nov 17 22:00:00 1996 Path: biosci!daresbury!imbb1.imbb.forth.gr!nefeli.imbb.forth.gr!SAAVEDRA From: saavedra@nefeli.imbb.forth.gr Newsgroups: bionet.molbio.bio-matrix,bionet.molbio.embldatabank,bionet.molbio.evolution Subject: Re: CAN TWO MEN (or women) HAVE KIDS??? Date: Tue, 19 Nov 1996 00:22:16 GMT Organization: Imbb-forth,CREtE, creece Lines: 51 Message-ID: <009AB90E.45F8A2F8@nefeli.imbb.forth.gr> References: <328E5E4F.DFC@mit.edu> Reply-To: saavedra@nefeli.imbb.forth.gr NNTP-Posting-Host: nefeli.imbb.forth.gr Xref: biosci bionet.molbio.bio-matrix:794 bionet.molbio.embldatabank:716 bionet.molbio.evolution:5339 Dear Bugra, There have been attempts to fuse sea urchin eggs. References that I know are Bennet and Mazia. 1981. Interspecific fusion of sea urchin eggs. Surface events and cytoplasmic mixing. J.exp.Res. 131: 197-207 Bennet and Mazia. 1981. Fusion of fertilized and unfertilized sea urchin eggs. Maintenance of cell surface integrity. J.exp.Res. 134: 494-498. In bivalves, Guo et al. (J. Shellfish Res. 13: 193-198, 1994) have tried to induce tetraploidy by fusing zygotes, sperm (to use in fertilization of normal eggs) and blastomeres. I would be nice to try fusion of eggs. The first problem I see is wether cell fusion alone would activate the development. Carlos Saavedra Dept. of Biology University of Crete P.O.Box 1470 711 10 Iraklio, Crete (Greece) Tel: +30 81 393 378 Fax: +30 81 239 155 In article <328E5E4F.DFC@mit.edu>, Bugra Giritlioglu writes: >I am curious to see what people know about the possibility of same-sex >parenting, i.e. the possibility of achieving fertilization by combining >the genetic material from two sperms (or two eggs). > >I recently corresponded with an expert on this topic, and to my >surprise, discovered that it may not be as impossible and crazy as it >sounds. (hi again, if you subscribe to any of these newsgroups!!) > >I am trying to get in touch with practically anybody who is excited >about this possibility and knows more about it than I do. > >My knowledge of the biology of reproduction is very hazy, however, I >would seriously consider going back to school in molecular genetics if I >knew that there were others interested in making this a reality. > >Is anybody out there doing research that might, directly or indirectly, >benefit this cause. Give me a holler!! > >Please help me get started if you have any ideas, suggestions, etc. > >Also, any suggestions as to where else I might try posting this message? From owner-embldatabank@net.bio.net Sun Nov 17 22:00:00 1996 Path: biosci!daresbury!imbb1.imbb.forth.gr!nefeli.imbb.forth.gr!SAAVEDRA From: saavedra@nefeli.imbb.forth.gr Newsgroups: bionet.molbio.bio-matrix,bionet.molbio.embldatabank,bionet.molbio.evolution Subject: Re: CAN TWO MEN (or women) HAVE KIDS??? Date: Tue, 19 Nov 1996 01:16:35 GMT Organization: Imbb-forth,CREtE, creece Lines: 60 Message-ID: <009AB915.DC1B7308@nefeli.imbb.forth.gr> References: <328E5E4F.DFC@mit.edu>, Reply-To: saavedra@nefeli.imbb.forth.gr NNTP-Posting-Host: nefeli.imbb.forth.gr Xref: biosci bionet.molbio.bio-matrix:795 bionet.molbio.embldatabank:717 bionet.molbio.evolution:5340 In article , cummins@central.murdoch.edu.au (Jim Cummins) writes: >In article <328E5E4F.DFC@mit.edu>, Bugra Giritlioglu wrote: > >> I am curious to see what people know about the possibility of same-sex >> parenting, i.e. the possibility of achieving fertilization by combining >> the genetic material from two sperms (or two eggs). > >This probably would not work as differential methylation during genomic >imprinting in the gonad treats the male genes differently from the >female. Male genes acting in the embryo serve to maximise growth and >placental function at the expense of the maternal organism. Female genes >serve to mimimise growth and preserve the maternal interest. One would >have to de-imprint the genes to have same-sex fertilization. Occasionally >one gets a dispermic egg developing without the egg's genes participating >- the end result if a hydatidiform mole - a monster. Ocasionally eggs >will activate spontaneously in the ovary and result in an ovarian >teratoma. So we DO need two genders! > >-- >URL http://numbat.murdoch.edu.au/spermatology/spermhp.html In the bivalve Mulinia lateralis, development of gynogenetic individuals obtained by activation of the egg with UV-irradiated sperm (DNA is destroyed) and blockage of polar body extrusion is normal. Polar body and egg should have different imprinting patterns than sperm, and probably share the same pattern between them. Your argument of imprinting is not valid. Actually, I wonder if imprinting has been demonstrated in other organisms than mammals (I think it hasn't). Gynogens in bivalves indicate that you do not need two genomes of different sexes to make a viable embryo. Maybe you need two sexes, if you consider that activation of the egg is carried out with sperm (although irradiated). But even that is not true: activation of the egg can be achieved by treatment with potassium chloride in Mulinia. Diploid bivalves could be obtained fusing eggs if the fusion activates development at the same time. Otherwise you would need sperm, potassium chloride or other means to activate the eggs before, while or after fusion has occurred. Nobody has tried that, I guess. I have to recognize that most attempts of obtaining viable gynogens have not been successful, because animals dye during the larval phase or earlier. But we do not know if that is due to the treatment or to more fundamental reasons. One of these fundamental reasons is simply that deleterous genes are put in ho ozygosis by gynogenesis. Carlos Saavedra P.S.: maybe it wouldbe better to continue with this in bionet.molbio.molluscs. That newsgroup neeeds some activating agent, and I don't believe that potassium chloride would help in this case! Carlos Saavedra Dept. of Biology Univ. of Crete P.O. Box 1470 711 10 Iraklio, Crete (Greece) Tel: +30 81 393 378 Fax: +30 81 239 155 From owner-embldatabank@net.bio.net Mon Nov 25 22:00:00 1996 Path: biosci!rutgers!uwm.edu!news-peer.gsl.net!news.gsl.net!news.sprintlink.net!news-peer.sprintlink.net!howland.erols.net!usc!newshub.csu.net!mercury!nthomas From: nthomas@mercury.calstatela.edu (Nigel J. Thomas) Newsgroups: bionet.molbio.embldatabank Subject: Molecular Biology Pioneers Date: 26 Nov 1996 11:26:53 GMT Organization: Information Resources and Technology Lines: 13 Message-ID: <57ek5t$9ug@nuke.csu.net> NNTP-Posting-Host: mercury.calstatela.edu I am looking for biographical information on Molecular Biology pioneers Maurice Wilkins and Max Perutz, known for their work on the X-ray crystallography of DNA and haemoglobin respectively. In particular, can anyone tell me if (or where I might look to find out if) either of them are still alive, and, if not, when and where did they die. However, any other biographical information (preferably with references) or sugestions as to where I might be able to look it up would be appreciated. Please reply by email as my newsgroup connection is a bit unreliable, and very clunky.. Thanks, Nigel From owner-embldatabank@net.bio.net Mon Nov 25 22:00:00 1996 Path: biosci!agate!howland.erols.net!news-peer.gsl.net!news.gsl.net!usenet.eel.ufl.edu!warwick!lyra.csx.cam.ac.uk!news From: David Philip Judge Newsgroups: bionet.general,bionet.molbio.embldatabank,embnet.general Subject: EBI, HGMP and Sanger temporarily out of contact. Date: Tue, 26 Nov 1996 13:38:40 +0000 Organization: Cambridge University. Lines: 23 Message-ID: <329AF2E0.167E@mole.bio.cam.ac.uk> NNTP-Posting-Host: jennings.bio.cam.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (X11; I; IRIX 5.3 IP22) CC: David Philip Judge Xref: biosci bionet.general:24225 bionet.molbio.embldatabank:723 Due to an unfortunate accident by one of the building contractors working at the Hinxton Hall Site, it is currently not possible to contact the European Bioinformatics Institute (EBI), the HGMP Resource Centre or the Sanger Cente either by telephone or over the network. At present, it looks like it might take several hours to resolve this difficulty. Apologies are offered for the inconvenience this break in service will cause. David Judge -- __________________________________________________________________ David P. Judge Tel : +44-1223-333614 Room 204, FAX : +44-1223-333992 Department of Genetics, Email : dpj10@mole.bio.cam.ac.uk University of Cambridge, WWW : http://jennings.bio.cam.ac.uk/ Tennis Court Road, CAMBRIDGE CB2 3EH, ENGLAND. __________________________________________________________________