From owner-embldatabank@net.bio.net Thu Apr 03 23:00:00 1997 Path: biosci!mail.only!postal From: postal@mail.only Newsgroups: bionet.molbio.embldatabank Subject: Search Engine Secrets Date: 3 Apr 1997 18:28:14 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 120 Sender: daemon@net.bio.net Distribution: world Message-ID: <199704040206.SAA20906@global1.global.net> NNTP-Posting-Host: net.bio.net Dear Friend and Fellow Entrepreneur, DISCOVER The Most Powerful & PROVEN Strategies that Really Work To Place You At The Top of the Search Engines! If you have a web page, or site, that can't be found at the top of the search engines then this will be the most important information you will ever read. Everyone know that the key to success on the web is positioning. You are about to Discover the most Powerful Strategies used only by the very best on the Web... strategies so Powerful that once used will place your Web Page or site at the TOP 10 - 20 search engine listings! These TOP SECRET strategies will provide you with a cutting edge advantage over your competition and give you the long awaited results you have been looking for. Just imagine opening a Floodgate of People into your Home Page because you have the right information. It doesn't matter if you have one page or 1000 pages, you can achieve a top rating with this powerful information and soon squash your competition! This in-depth report covers: 7 Search Engine Tactics your competition doesn't want you to know! 7 The best kept secrets to getting you a top 10 - 20 listing! 7 The 10 top keywords searched for! 7 Getting better positioning than your opposition even when they have the same identical keywords! 7 Proven techniques for selecting the most effective keywords and how to arrange them! 7 A powerful way to get your listing seen by potential customers, even if they're not looking for you! 7 A little-known way to get multiple listings for your site in the same search engine! 7 Proven strategies used to resubmit your page or site and get that top rating even if you have it listed already! 7 How to get people to go to your site first even if they see your competition! 7 The most powerful words used to create the best Web Pages! 7 A Web tool used to market successfully in the Newsgroups! 7 Five things you should NEVER do! If you aren't at the top of the search engines now... your competition is! It's estimated over 1000 new Web Pages are coming online every day! Newspapers are reporting over 14,000 new www addresses are being submitted every week. The competition grows every minute! It just makes sense, that those who know and apply this information will definitely have the best chance of realizing their dreams of success. This in-depth report is normally US$49.95... However, if you order within the next 10 days... we'll include ABSOLUTELY FREE... OVER 1000 Links where you can advertise your web site FREE and you can have it all for JUST US$19.95! This INVALUABLE information alone is worth the asking price! Don't delay...this Extraordinary and Valuable Information can be yours today for ONLY $19.95 (USA FUNDS). Why Wait...Order Right Now! As an added BONUS, if you respond within 10 days: You'll also receive free tools, images, and tips to help you with Your Web Page construction, including free CGI scripts, buttons, backgrounds, and loads of Jpegs and Gifs, including animated Gifs! Please print, cut, and fill out the following order coupon: ---------------------------------------------------------------------------- Name __________________________________________________________ E-mail Address __________________________________________________________ Address __________________________________________________________ City ______________________ State ________ Zip ___________ Country______________________ Phone #______________________ SEARCH ENGINE SECRETS $19.95 Sales Tax (MA residents 5.00%) ______ Order total US $______ PAYMENT BY: ___ Check ___ Money Order ___ Cashiers Check - US FUNDS only! >>If you're ordering from outside the USA, only a Money Order in US Dollars will be accepted. No postal delivery is available outside the USA, so you must include your E-mail address accurately and legibly.<< ---------------------------------------------------------------------------- For fastest service use Cashiers Check or Money Order. Please include your e-mail address for 24 hour order processing upon clearance of funds. Please allow 2-3 weeks for processing by regular postal mail. Please make payable to -> EVA, Inc. and send to: EVA, Inc. 43 Riverside Ave., #72 Medford, MA 02155 USA Reminder: Your order must be postmarked by Monday April 14th in order to receive the bonuses and be eligible for the discount from the regular price of $49.95! ******************************************************************* sent you this message using WorldMerge, the fastest and easiest way to send personalized email messages to your customers, subscribers, leads or friends. Download your free copy today! ******************************************************************* From owner-embldatabank@net.bio.net Thu Apr 03 23:00:00 1997 Path: biosci!mail.only!postal From: postal@mail.only Newsgroups: bionet.molbio.embldatabank Subject: Search Engine Secrets Date: 4 Apr 1997 08:29:04 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 120 Sender: daemon@net.bio.net Distribution: world Message-ID: <199704041626.LAA06867@ns.newreach.net> NNTP-Posting-Host: net.bio.net Dear Friend and Fellow Entrepreneur, DISCOVER The Most Powerful & PROVEN Strategies that Really Work To Place You At The Top of the Search Engines! If you have a web page, or site, that can't be found at the top of the search engines then this will be the most important information you will ever read. Everyone knows that the key to success on the web is positioning. You are about to Discover the most Powerful Strategies used only by the very best on the Web... strategies so Powerful that once used will place your Web Page or site at the TOP 10 - 20 search engine listings! These TOP SECRET strategies will provide you with a cutting edge advantage over your competition and give you the long awaited results you have been looking for. Just imagine opening a Floodgate of People into your Home Page because you have the right information. It doesn't matter if you have one page or 1000 pages, you can achieve a top rating with this powerful information and soon squash your competition! This in-depth report covers: · Search Engine Tactics your competition doesn't want you to know! · The best kept secrets to getting you a top 10 - 20 listing! · The 10 top keywords searched for! · Getting better positioning than your opposition even when they have the same identical keywords! · Proven techniques for selecting the most effective keywords and how to arrange them! · A powerful way to get your listing seen by potential customers, even if they're not looking for you! · A little-known way to get multiple listings for your site in the same search engine! · Proven strategies used to resubmit your page or site and get that top rating even if you have it listed already! · How to get people to go to your site first even if they see your competition! · The most powerful words used to create the best Web Pages! · A Web tool used to market successfully in the Newsgroups! · Five things you should NEVER do! If you aren't at the top of the search engines now... your competition is! It's estimated over 1000 new Web Pages are coming online every day! Newspapers are reporting over 14,000 new www addresses are being submitted every week. The competition grows every minute! It just makes sense, that those who know and apply this information will definitely have the best chance of realizing their dreams of success. This in-depth report is normally US$49.95... However, if you order within the next 10 days... we'll include ABSOLUTELY FREE... OVER 1000 Links where you can advertise your web site FREE and you can have it all for JUST US$19.95! This INVALUABLE information alone is worth the asking price! Don't delay...this Extraordinary and Valuable Information can be yours today for ONLY $19.95 (USA FUNDS). Why Wait...Order Right Now! As an added BONUS, if you respond within 10 days: You'll also receive free tools, images, and tips to help you with Your Web Page construction, including free CGI scripts, buttons, backgrounds, and loads of Jpegs and Gifs, including animated Gifs! Please print, cut, and fill out the following order coupon: ---------------------------------------------------------------------------- Name __________________________________________________________ E-mail Address __________________________________________________________ Address __________________________________________________________ City ______________________ State ________ Zip ___________ Country______________________ Phone #______________________ SEARCH ENGINE SECRETS $19.95 Sales Tax (MA residents 5.00%) ______ Order total US $______ PAYMENT BY: ___ Check ___ Money Order ___ Cashiers Check - US FUNDS only! >>If you're ordering from outside the USA, only a Money Order in US Dollars will be accepted. No postal delivery is available outside the USA, so you must include your E-mail address accurately and legibly.<< ---------------------------------------------------------------------------- For fastest service use Cashiers Check or Money Order. Please include your e-mail address for 24 hour order processing upon clearance of funds. Please allow 2-3 weeks for processing by regular postal mail. Please make payable to -> EVA, Inc. and send to: EVA, Inc. 43 Riverside Ave., #72 Medford, MA 02155 USA Reminder: Your order must be postmarked by Monday April 14th in order to receive the bonuses and be eligible for the discount from the regular price of $49.95! ******************************************************************* sent you this message using WorldMerge, the fastest and easiest way to send personalized email messages to your customers, subscribers, leads or friends. Download your free copy today! ******************************************************************* From owner-embldatabank@net.bio.net Sat Apr 05 23:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.embldatabank Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 6 Apr 1997 03:00:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199704061000.DAA19326@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-embldatabank@net.bio.net Mon Apr 14 23:00:00 1997 From: "Orlando" Subject: Information needed! Newsgroups: bionet.molbio.embldatabank Message-ID: <01bc4950$5817d720$793509c8@orlando> X-Newsreader: Microsoft Internet News 4.70.1155 NNTP-Posting-Host: apolo6-a26.racsa.co.cr Date: 14 Apr 97 16:57:37 GMT Lines: 7 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news-peer.sprintlink.net!news.sprintlink.net!sprint!worldnet.att.net!newsfeed.internetmci.com!ns.racsa.co.cr! I work for an offshore bank and Iīm interested in learning how a Payable Through Bank in U.S.A. works and the general basis of the Offshore Banking. I would like to have some information about these topics and specially concerning to wire transfers because thatīs part of my job. You can send me the information through this newsgroup or through the e-mail: orlrodri@sol.racsa.co.cr From owner-embldatabank@net.bio.net Tue Apr 22 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!news.maxwell.syr.edu!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!ais.net!uunet!in3.uu.net!202.232.2.100!np1.iij.ad.jp!nf0.iij.ad.jp!nr0.iij.ad.jp!news.iij.ad.jp!rim.or.jp!news.kddnet.ad.jp!news3.intervia!news2.tokyo.mbn!news.tokyo.mbn!not-for-mail From: "Tomohiro Yoshizawa" Newsgroups: alt.books.iain-banks,alt.invest.penny-stocks,alt.invest.real-estate,alt.invest.technical-analysis.omega,alt.private.investigator,asu.investigates.info-highway,aus.invest,bionet.molbio.embldatabank,bionet.molbio.genbank,bionet.molbio.genbank.updat Subject: Where is the Internet Bank? Date: 23 Apr 1997 21:42:29 GMT Organization: Media,Tokyo,JAPAN Lines: 10 Message-ID: <01bc502e$1370ab80$LocalHost@tomo> NNTP-Posting-Host: cse-9.kofu.mbn.or.jp Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-2022-JP Content-Transfer-Encoding: 7bit X-Newsreader: Microsoft Internet News 4.70.1157 Xref: biosci bionet.molbio.embldatabank:813 bionet.molbio.genbank:2557 Hello! I am interested in Internet Banking. I would like to have my bank account in Hong Kong, US, or Swiss although I live in Japan now. So I am looking for the Home Page which I can apply the bank account by the Internet. Please teach me the where I can apply the bank account by the Internet. Please send to my e-mail address. Any information would be very appreciated. Thank you. Tomohiro From owner-embldatabank@net.bio.net Tue Apr 22 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!news.maxwell.syr.edu!ais.net!uunet!in3.uu.net!202.232.2.100!np1.iij.ad.jp!nf0.iij.ad.jp!nr0.iij.ad.jp!news.iij.ad.jp!rim.or.jp!news.kddnet.ad.jp!news3.intervia!news2.tokyo.mbn!news.tokyo.mbn!not-for-mail From: "Tomohiro Yoshizawa" Newsgroups: alt.books.iain-banks,alt.invest.penny-stocks,alt.invest.real-estate,alt.invest.technical-analysis.omega,alt.private.investigator,asu.investigates.info-highway,aus.invest,bionet.molbio.embldatabank,bionet.molbio.genbank,bionet.molbio.genbank.updat Subject: Where can I do the Commodity Future trading in the Internet? Date: 23 Apr 1997 21:42:31 GMT Organization: Media,Tokyo,JAPAN Lines: 11 Message-ID: <01bc502e$f3aab560$LocalHost@tomo> NNTP-Posting-Host: cse-9.kofu.mbn.or.jp Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-2022-JP Content-Transfer-Encoding: 7bit X-Newsreader: Microsoft Internet News 4.70.1157 Xref: biosci bionet.molbio.embldatabank:814 bionet.molbio.genbank:2558 Hello! I am interested in the Internet Futures Commodities trading. I would like to trade in the foreign market although I live in Japan now. So I am looking for the Home Page which I can apply the Commodity Future trading by the Internet. Please teach me the where I can apply the Commodity Future trading by the Internet. Please send to my e-mail address. Any information would be very appreciated. Thank you. Tomohiro From owner-embldatabank@net.bio.net Mon Apr 28 23:00:00 1997 Path: biosci!webtv.net!uunet!in1.uu.net!207.172.3.52!feed1.news.erols.com!howland.erols.net!ix.netcom.com!news From: Kevin Shire Newsgroups: alt.books.iain-banks,alt.invest.penny-stocks,alt.invest.real-estate,alt.invest.technical-analysis.omega,alt.private.investigator,asu.investigates.info-highway,aus.invest,bionet.molbio.embldatabank,bionet.molbio.genbank,bionet.molbio.genbank.updat Subject: Re: Where can I do the Commodity Future trading in the Internet? Date: Mon, 28 Apr 1997 21:40:34 -0700 Organization: Netcom Lines: 20 Message-ID: <33657BC2.40F3@ix.netcom.com> References: <01bc502e$f3aab560$LocalHost@tomo> NNTP-Posting-Host: sac-ca22-02.ix.netcom.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-NETCOM-Date: Mon Apr 28 10:39:51 PM PDT 1997 X-Mailer: Mozilla 3.01 (Win16; I) Xref: biosci bionet.molbio.embldatabank:819 bionet.molbio.genbank:2563 Tomohiro Yoshizawa wrote: > > Hello! I am interested in the Internet Futures Commodities > trading. I would like to trade in the foreign market > although I live in Japan now. So I am looking for the Home > Page which I can apply the Commodity Future trading by the > Internet. > > Please teach me the where I can apply the Commodity Future > trading by the Internet. Please send to my e-mail address. > Any information would be very appreciated. Thank you. > > Tomohiro Mr. Tomohiro: My name is Kevin Shire, I am a commodities broker in Sacramento Ca. I could make all the arrangements for you to open a U.S. bank account and trade commodities futures with me through my company, so please take this under advisement and I hope to here from you soon!! From owner-embldatabank@net.bio.net Mon Apr 28 23:00:00 1997 Newsgroups: bionet.molbio.embldatabank Path: biosci!bcm.tmc.edu!cs.utexas.edu!news.maxwell.syr.edu!uknet!usenet1.news.uk.psi.net!uknet!uknet!lyra.csx.cam.ac.uk!hgmp.mrc.ac.uk!ebi.ac.uk!news From: "Matteo R. diTommaso" Subject: TIGR EST Sequences Sender: news@ebi.ac.uk (usenet news) Message-ID: X-Priority: 3 (Normal) Date: Mon, 28 Apr 1997 23:59:48 GMT Lines: 25 Content-Type: TEXT/PLAIN; charset=US-ASCII Mime-Version: 1.0 Organization: European Bioinformatics Institute X-Newsreader: Chameleon Forum Client v. 6.0.1, NetManage Inc. The 91,000+ EST sequences recently submitted to GenBank/EMBL/DDBJ by The Institute For Genomic Research (TIGR) are now available from the EBI ftp server in GenBank flatfile format. An EMBL format file will be available shortly. You will find the file at: ftp://ftp.ebi.ac.uk/pub/databases/embl/TIGR_EST/ tigr_est_0497.gbf.Z this is the same file which can be found at the NCBI: ftp://ncbi.nlm.nih.gov/genbank/daily-nc tigr.est.0497.gbf.Z Although these sequences will be made available as part of the EMBL Release 51 (June 1997) in the EST divisions, we will not immediately release them with the normal daily and cumulative updates because of the size of the submission (>50MBytes). ------------------------------------- Matteo R. diTommaso The European Bioinformatics Institute tommaso@ebi.ac.uk 04/28/97 23:59:48 ------------------------------------- From owner-embldatabank@net.bio.net Tue Apr 29 23:00:00 1997 Path: biosci!daresbury!uninett.no!nntp.uio.no!news.uoregon.edu!newsfeed.orst.edu!news.orst.edu!ava.bcc.orst.edu!fieldk From: fieldk@ava.bcc.orst.edu (Katharine Field) Newsgroups: bionet.molbio.embldatabank Subject: Molecular Methods Workshop Course Date: 30 Apr 1997 00:11:45 GMT Organization: Oregon State University Lines: 160 Message-ID: <5k62o1$35o@news.orst.edu> NNTP-Posting-Host: ava.bcc.orst.edu Announcing a workshop on Methods in Molecular Biology at Oregon State University An intensive training course for graduate students, faculty and researchers in the biological and medical sciences offered by the Molecular and Cellular Biology Program Oregon State University Corvallis, OR (541) 737-3799 The methods of molecular biology, in particular recombinant DNA, have transformed research in the biological and medical sciences. This technology currently influences all aspects of biological research, has far-reaching applications in clinical diagnosis, and has led to economically important developments in agriculture and biotechnology. This course is designed to provide a hands-on introduction to molecular biological methods. Participants will spend two weeks in intensive laboratory work. In addition, daily lectures by molecular biologists will provide theoretical background and insight into cutting-edge research developments and techniques. Participants will learn the following techniques: cloning vector manipulation the polymerase chain reaction (PCR) preparation of recombinant DNA transformation of bacterial cells isolation of genomic DNA and RNA restriction analysis handling of radioactive isotopes isotopic and non-isotopic probe labelling Southern blots site-directed mutagenesis northern blots DNA sequencing synthesis of a fusion protein protein expression western blots yeast two-hybrid screening Prerequisites: An up-to-date background in DNA structure and chemistry, genetics and biochemistry. Schedule Session 1: July 14-26, 1997 Session 2: September 8-20, 1997 This is a highly intensive training course, requiring a full-time commitment from the participants. Activities are scheduled all day and some evenings. Classes begin in the laboratory at 8:00 AM. A formal lecture is held at 1:00 PM each day. There will be one Sunday off in the middle of session. Registration, Fees & Credit Registration will be limited to 24 students per session. A limited number of places in the course will be reserved for MCB students and other OSU graduate students who are required to take the course as part of their programs. All other course participants will be charged a workshop fee of $750 to take the course, or $900 to take the course and receive 3 credits through Continuing Higher Education (CHE) at Oregon State University. Lectures Only If you are not taking the lab course, you may sign up for the lecture portion only, MCB 524. MCB 524 is offered as a 1-credit, pass/no pass course through the summer school (July session) or as a fall-term course (September). There is no limit on enrollment. The course consists of approximately 15 one-hour lectures presented by faculty of the Molecular and Cellular Biology Program. MCB 525 students may not take MCB 524 concurrent with MCB 525. MCB 525 Staff Dr. Katharine Field, Coordinator Dr. Walt Ream, Co-coordinator (September) Dr. Gary Merrill, Co-coordinator (July) Housing Housing in studio "quad" apartments (single rooms with shared kitchen and bath) is available close to campus at a cost of approximately $185 for two weeks in July and $190 for two weeks in September. Restaurants are a short distance from class. A campus parking permit can be purchase for $5 per week. Class participants can use Dixon Recreation Center (lockers, swimming, weight room, outdoor track, and more) for $3.75 per day or $14 per week. Location The class will be held in the new Agricultural and Life Sciences Building on the Oregon State University Campus in Corvallis. Corvallis is a community of 45,000 residents located in the beautiful Willamette River Valley, an hour from the Pacific Coast and the Cascade Range. Important!! Graduate students who are required to take MCB 525 as part of their graduate program must provide a copy of their official program, or a letter from their major professor, along with the application, in order to be considered for the course. Workshop fees must be paid in full by two weeks before the first day of class! Further questions? Please call (541) 737-3799 or email - mcb@bcc.orst.edu -------------------------------------------------------- MCB 525 APPLICATION Please fill out and return by May 20, 1997. You will be notified if you have been accepted and registration forms will be mailed to you. You do not need to send in an application if you will be taking the lecture course only. Name: Position: Organization /Dept/P.I.'s name Highest Degree & date Address City State Zip Phone (work) (home) email address Preferred session July 14 - 26, 1997 September 8-20, 1997 Status Graduate Course for credit/program Workshop Course - no credit ($750) Workshop Course - 3 credits from CHE ($900) If you do not get into your preferred session, do you want to be considered for the other session? Yes No I will need housing Yes No Return to: MCB 525 Application Molecular and Cellular Biology Program 3021 Agricultural 7 Life Sciences Bldg. Oregon State University Corvallis, OR 97331-7303 or send by e-mail to: mcb@bcc.orst.edu From owner-embldatabank@net.bio.net Tue Apr 29 23:00:00 1997 Newsgroups: embnet.general,bionet.general,bionet.molbio.embldatabank Path: biosci!daresbury!lyra.csx.cam.ac.uk!hgmp.mrc.ac.uk!ebi.ac.uk!news From: "Matteo R. diTommaso" Subject: Re: TIGR EST Sequences X-Internet-To: ditommaso@ebi.ac.uk, etzold@ebi.ac.uk, "Kruszewska.Kasia" , lopez@ebi.ac.uk Sender: news@ebi.ac.uk (usenet news) Message-ID: X-Priority: 2 (High) Date: Tue, 29 Apr 1997 17:05:13 GMT Lines: 30 Content-Type: TEXT/PLAIN; charset=US-ASCII References: Mime-Version: 1.0 Organization: European Bioinformatics Institute X-Newsreader: Chameleon Forum Client v. 6.0.1, NetManage Inc. Xref: biosci bionet.general:26612 bionet.molbio.embldatabank:822 The 91,000+ EST sequences recently submitted to GenBank/EMBL/DDBJ by The Institute For Genomic Research (TIGR) are now available from the EBI ftp server in EMBL and GenBank flatfile formats. EMBL Format: ftp://ftp.ebi.ac.uk/pub/databases/embl/TIGR_EST/ tigr_est_0497.embl.Z GenBank Format: ftp://ftp.ebi.ac.uk/pub/databases/embl/TIGR_EST/ tigr_est_0497.gbf.Z The GenBank format file is the same file which can be found at the NCBI: ftp://ncbi.nlm.nih.gov/genbank/daily-nc tigr.est.0497.gbf.Z Although these sequences will be made available as part of the EMBL Release 51 (June 1997) in the EST divisions, we will not immediately release them with the normal daily and cumulative updates because of the size of the submission (>50MBytes compressed). For the EBI SRS servers we will treat this dataset as a second division of EMNEW. We should have this dataset indexed and available through SRS within a day. Matteo diTommaso -------------------------------------------------------- E-mail: ditommaso@ebi.ac.uk 04/29/97 17:05:14 WWW: http://jupiter.ebi.ac.uk/~tommaso/ EMBL Outstation - European Bioinformatics Institute Hinxton Hall, Hinxton, Cambs, CB10 1SD United Kingdom --------------------------------------------------------