From owner-embldatabank@net.bio.net Sun Nov 02 22:00:00 1997 Path: biosci!daresbury!uninett.no!newscore.univie.ac.at!europa.clark.net!205.252.116.205!howland.erols.net!ix.netcom.com!news From: IntimateSVC@GetPaid.com Newsgroups: bionet.molbio.embldatabank Subject: Women Pay You For Intimate Services Date: Sun, 2 Nov 1997 22:07:02 Organization: Netcom Lines: 12 Message-ID: <63jf49$iqe@dfw-ixnews11.ix.netcom.com> NNTP-Posting-Host: haw-ak1-04.ix.netcom.com X-NETCOM-Date: Sun Nov 02 9:06:49 PM CST 1997 GIRLS PAY YOU FOR YOUR SPECIAL INTIMATE SERVICES Men around the country are making $500 plus weekly in their spare time providing services to lonely women in need. This service can be provided by men just like you. Anyone can provide these services, race, age (over18) , and looks do not matter. Send $2.00 cash for details via Snail Mail, or send $1 cash for details via your E-Mail address. Services, 811 Flushing Ave. Brooklyn, NY 11206 C/O Jeffrey Hardman From owner-embldatabank@net.bio.net Wed Nov 05 22:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.embldatabank Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 6 Nov 1997 10:07:09 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Distribution: world Message-ID: <199711061000.CAA24115@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-embldatabank@net.bio.net Thu Nov 06 22:00:00 1997 Path: biosci!daresbury!uninett.no!sn.no!news-feed.ifi.uio.no!recycled.news.erols.com!howland.erols.net!newsfeed.internetmci.com!199.0.65.142!news-feed1.tiac.net!news-master.tiac.net!news@tiac.net From: propdig Newsgroups: bionet.molbio.embldatabank Subject: National Biotech Register(NatBio) New Service Date: Fri, 07 Nov 1997 14:14:30 -0500 Organization: propdig Lines: 5 Message-ID: <34636896.2E8E@barryinc.com> Reply-To: propdig@barryinc.com NNTP-Posting-Host: p2.ts4.lowel.ma.tiac.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0C-NSCP (Win95; U) NatBio, http://www.barryinc.com/bio is pleased to introduce a new service on our web site. A Biotech Industry Company profile search. Are you looking for information on a particular Biotech company? We probably have it. We also invite you to check out your own company. If you are not listed, email us the data and we will add it for free. From owner-embldatabank@net.bio.net Fri Nov 07 22:00:00 1997 Path: biosci!USERS.AFRICAONLINE.CO.KE!wellcome From: wellcome@USERS.AFRICAONLINE.CO.KE ("Wellcome Trust, Nairobi.") Newsgroups: bionet.molbio.embldatabank Subject: Long PCR primers Date: 8 Nov 1997 05:40:13 -0800 Organization: Wellcome Trust Research Laboratories Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: <34650219.2747@users.AfricaOnline.Co.Ke> Reply-To: wellcome@users.africaonline.co.ke NNTP-Posting-Host: net.bio.net Dear Colleagues, If there any risk of amplifying a fragment DNA with primer which are 50 to 60 pb. Actually, I want to introduce a enzyme restriction site in these primers so that I can easily detected them in agore or Acrylamide gel. Therefore I do need longer primers. But I wandering about doing a PCR such long oligonucleotides. My regards, -- Wellcome Trust Research Labs, PO Box 43640, Nairobi, Kenya. Tel 254 (2) 725390, Fax 254 (2) 711673 E-mail: wellcome@users.africaonline.co.ke From owner-embldatabank@net.bio.net Tue Nov 11 22:00:00 1997 Path: biosci!rutgers!uwm.edu!newsfeeds.sol.net!europa.clark.net!206.229.87.25!news-peer.sprintlink.net!news-peer-east.sprintlink.net!news.sprintlink.net!Sprint!rill.news.pipex.net!pipex!join.news.pipex.net!pipex!server1.netnews.ja.net!hgmp.mrc.ac.uk!ebi.ac.uk!stoehr From: stoehr@ebi.ac.uk (Peter Stoehr) Newsgroups: bionet.molbio.embldatabank,embnet.general Subject: EMBL Release 52 available Date: 12 Nov 97 18:29:40 +0000 Organization: European BioInformatics Institute Lines: 124 Distribution: embnet Message-ID: <1997Nov12.182940@ebi.ac.uk> NNTP-Posting-Host: mars.ebi.ac.uk Release 52 of the EMBL Nucleotide Sequence Database is available from the EBI FTP server. FTP server Directory ----------- --------- ftp.ebi.ac.uk pub/databases/embl/release EMBL flat-file format pub/databases/embl/release/gcg GCG8.x standard format Some extracts from the release notes are appended below. Fuller details in: ftp://ftp.ebi.ac.uk/pub/databases/embl/release/relnotes.doc The cumulative update file pub/databases/embl/new/cumulative.dat.Z will be changed at Thursday 10-NOV-1997 15:00 GMT to include only data created since release 52. Regards, Peter Stoehr EMBL-EBI ======== The EMBL Nucleotide Sequence Database was frozen to make Release 52 on the 17th October 1997. The release contains 1,787,004 sequence entries comprising 1,181,167,498 nucleotides. This represents an increase of about 26% over Release 51. A breakdown of Release 52 by division is shown below: Division Entries Nucleotides ----------------- ------- ----------- Bacteriophage 1351 2132530 ESTs 1282047 472985339 Fungi 17338 43598348 GSSs 46472 24917886 HTG 1300 89642076 Human 71873 124647874 Invertebrates 27362 105437150 Organelles 23449 22009199 Other Mammals 13715 13190473 Other Vertebrates 12732 14134235 Plants 21031 34469699 Patent 90819 29402838 Prokaryotes 40292 90331355 Rodents 36085 44729959 STSs 50721 17472981 Synthetic 2382 5192851 Unclassified 2540 2377434 Viruses 45495 44495271 ----------------- -------- ------------ Total 1787004 1181167498 ----------------- -------- ------------ 1.1 EST Database Files In order to keep the size of the data files within reasonable limits for handling purposes, we have split the EST division into several files. At this release we have created two extra files of EST data named EST12.DAT and EST13.DAT. Additional files will be added in subsequent releases as appropriate. 1.2 Patent Sequences In PATENT.DAT At this release we have grouped all entries derived from the patent literature into one file PATENT.DAT. Previously we had merged such data into the other divisions based on taxonomy, and PATENT.DAT only contained sequences which were shorter than 15bp and which were known to be duplicates or reverse complements of existing patent sequences. 2 FORTHCOMING CHANGES 2.1 Nucleotide And Protein Identifiers 2.1.1 Nucleotide Indentifiers The NI linetype of the EMBL flat-file format currently contains a unique identifier for the nucleotide sequence. While the sequence remains the same, so does the value of this identifier. When a sequence change occurs, however minor, a new NI value will be assigned whilst the accession number on the AC line may remain unchanged. These identifiers are collaboratively maintained with GenBank and DDBJ, for example: NI g21954 It has become clear from users and other database groups that confusion has been created about the relationship between these identifiers and the GenBank 'gi' numbers. It has been decided therefore to introduce a new system of nucleotide identifiers of the form 'accession.version', eg: X12345.3, where the accession number part will be stable, but the version part will be incremented if the sequence changes. Subject to synchronisation of this change with GenBank and DDBJ, we plan to implement this new form of nucleotide identifier at release 54, March 1998. 2.1.2 Protein Identifiers Protein identifiers are currently assigned to all CDS features in the nucleotide sequence database and are found in the feature table qualifier /db_xref, eg: /db_xref="PID:e123456789" As for nucleotide identifier values (above), confusion resulted amongst users concerning the relationship of these to GenBank 'gi' numbers also assigned to CDS features. To clarify this, and to adopt a comparable scheme of identifiers for both nucleotides and proteins, the collaborating databases have decided to create a new feature table qualifier /protein_id, eg: /protein_id="AAA12345.1" This form of identifier also allows easier tracking of changing protein identifiers by external databases than the previous PIDs. This qualifier consists of a stable ID portion (3+5 format with 3 positions letters and 5 numbers) plus a version number after a decimal point. The version number will change only when the protein sequence coded by the CDS changes, while the stable part will remain unchanged. This qualifier will be valid only on CDS features which translate into a valid protein. Subject to synchronisation of this change with GenBank and DDBJ, we plan to implement this new form of protein identifier at release 54, March 1998.