From owner-gene-linkage@net.bio.net Sun Aug 02 23:00:00 1998 Path: biosci!SLIP.NET!grizzly From: grizzly@SLIP.NET (Michael Sherrell) Newsgroups: bionet.molbio.gene-linkage Subject: Sequencers/synthesizers Date: 3 Aug 1998 16:05:59 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 55 Sender: daemon@net.bio.net Distribution: world Message-ID: <01BDBEF8.4279FE60@oak-pm1-50-178.dialup.slip.net> NNTP-Posting-Host: net.bio.net I have a number of peptide and oligo synthesizers and sequencers for = sale: Licor 4200S-2 two-laser Sequencer, 4 mos. old Licor 4000L sequencer, ~$30K PerSeptive 9050, ~$6K PerSeptive Expedite 8909, <=3D $14K ABI 394, rebuilt, warranteed, Oligonet-ready, ~$12K ABI 394, rebuilt, warranteed, non-Oligonet, ~$11K ABI 391, rebuilt, warranteed, $7K ABI 430, rebuilt, warranteed, ~$12K ABI 431, ~$14K ABI 432 Synergy ABI 433, rebuilt by ABI, warranteed, < $40K ABI 373 classic, $10,500 ABI 373 stretch, 5-filter, 36-lane, $29K ABI 477, <=3D $10K ABI Catalyst 800 ABI 120, 130, $2.5K [We rebuild valve blocks for ABI 430, 431 and 39x @ $65/port, 90-day = warranty] also: LC-MS: Finnigan TSQ 7000, ~$200K Fisons VG 2000, <$100K Finnigan MAT 900, <$50K Finnigan MAT 90, ~$45K =09 MALDI-TOF: HP, masses to 500 KDa, lightly used, <=3D$60K Finnigan Laser MAT 2000, <$40K Other expensive hi-tech items: Bio-Dot sub-microliter 8-channel aspirate/dispense system (typically = 96-well microplate source, glass slide, microwell plate or membrane = target), < 1 year old Molecular Devices 445SI-MAC Phosphorimager, ~ 3 years old, currently = operating and under maintenance contact I also have available a few other synthesizers and sequencers and a = wide selection of HPLCs, mass specs, robots and other lab instruments. Please contact me to discuss any of these items, or if you have any = items you might like to sell. Michael Sherrell Grizzly Analytical (USA) 707 887 2919/fax 707 887 9834 www.grizzlyanalytical.com From owner-gene-linkage@net.bio.net Thu Aug 06 23:00:00 1998 Path: biosci!news.stanford.edu!newsfeed.concentric.net!newshub.northeast.verio.net!sunqbc.risq.qc.ca!news-peer-east.sprintlink.net!news-peer.sprintlink.net!news-backup-west.sprintlink.net!news.sprintlink.net!199.224.117.12!news-xfer.epix.net!news1.epix.net!epix-news From: "Peter J. Floriani, Ph.D." Newsgroups: bionet.molbio.gene-linkage,bionet.molbio.gene-org Subject: Re: What is 3'-5' and 5'-3'? Date: Thu, 06 Aug 1998 20:48:48 -0700 Organization: epix Internet Services Lines: 54 Message-ID: <35CA7920.6FFC@epix.net> References: <35BF5288.4EBBFEE1@library.utoronto.ca> NNTP-Posting-Host: lspt-66ppp217.epix.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.02E-KIT (Win16; U) Jeff Heeney wrote: > A book entitled dictionary of science states "The two strands run in > opposite directions, 3'-5' on one strand corresponding to 5'-3' on the > other. > What the hell are they talking about?! > Sorry, specifically...what is the meaning of the ' character. > Thanks. > You can go back to doing something serious now. It's a serious question - so I'll try answering it. Seriously! The numbers are the numbers of the carbons of the deoxyribose (sugar) part of the DNA (remember that means deoxyRIBOnucleic acid). These carbon numbers are marked with a prime to distinguish them from the carbons which are in the purine or pyrimidine part - these are the famous A, C, G, T "bases" you've heard about. The carbons in those are numbered, but not marked with a prime. The prime distinguishes the carbons in the ACGT part from those in the sugar. Now both the carbon called five-prime and the carbon called three-prime have OH groups attached. These are the places by which one nucleoside (that means a purine or pyrimidine stuck to the sugar) is stuck to another. Either of these places can be connected to a phosphate (PO4), and then the unit is called a nucleotide. The three-prime carbon of one base may be connected to a phosphate and that same phosphate may be connected to the five-prime carbon of another base. This connection is called a phosphodiester bond. So, DNA is a string (sorry, that's my computer science showing) DNA is a polymer of nucleotides. Clearly, both the five-prime and the three-prime carbons of each nucleotide (except for the first and last) are connected to other nucleotides. The "ends" are different, since one has a free five-prime carbon (the 5' end) and the other has a free three-prime carbon (the 3' end). Like this: base base base base 5'-sugar-3'-PO4-5'-sugar-3'-PO4-5'-sugar-3'-PO4-5'-sugar-3' (I hope that shows the "base" on top of the "sugar" - if not, just use your imagination.) For more details, see the nearest available organic chemistry book. One I've used is "Biochemistry" by J. David Rawn, p. 627-629 and 665-668. Sincerely, Peter J. Floriani, Ph.D. floriani@epix.net ==================================================================== "I have often thanked God for the telephone." G. K. Chesterton, 1910 ==================================================================== From owner-gene-linkage@net.bio.net Fri Aug 07 23:00:00 1998 Path: biosci!news.stanford.edu!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-peer.gip.net!news.gsl.net!gip.net!portc01.blue.aol.com!audrey01.news.aol.com!not-for-mail From: ateasd5941@aol.com (ATeasd5941) Newsgroups: bionet.molbio.gene-linkage Subject: Genetic Cures of Insurance Disasters? Lines: 60 Message-ID: <1998080812414300.IAA28538@ladder01.news.aol.com> NNTP-Posting-Host: ladder01.news.aol.com X-Admin: news@aol.com Date: 8 Aug 1998 12:41:43 GMT Organization: AOL, http://www.aol.co.uk If you believe that genetics is the way forward in finding a cure, how do you think medicine would be developed from such discoveries? Have you considered the power of the insurance companies? Who are the developments worth more to, the pharmaceutical companies or the insurance companies? If the insurance companies got hold of results ( which they will do eventually) then couples will be forced to undergo screening of foetuses. What about the ethical issues and the trauma of abortions for women? Who helps you to cope with being told that you are going to get a terminal disease, because the screening WILL come before a cure? What is really being developed mass screening programs or cures? If a screening program is used then the cures will not be needed, it is cheaper to get rid of the imperfection to start with than to put it right. ( funny thing this because left to its own devices nature does this anyway) Governments will be left with no option once put under pressure to use the cheapest option. Is genetic medicine going to be used to vet employees and force them into lower status work? Are Governments going to use it to cut health bills ( anything is possible in the future)? Are you helping to finance a system that is going to label you and your family second class citizens? While everyone is caught up in their enthusiasm have they thought about some of little boys and girls ( also known as scientists) understanding the sociological consequences of what they discover? You wouldn't give a child a chemistry set to play with unattended. Who's attending these ' scientists ' at work? Who do you honestly think is going to be putting right the wrong once it happens, whether it's the pressure of the insurance companies, governmental, medical or the pharmaceutical companies or any physical damage? I am sorry if I am shattering the illusion under which your hopes are pinned, but put some thought to this. I also know that there are many medical professionals who agree with me, but they just don't have the guts to stand forward from the rest for what they believe in. When medicine is used to some of these ends then it is no longer a healing art to be respected, it becomes a source of distress and illness for many people. Information has the power to transform things and make them better, but only if the information is truly understood. No offline discussion about this please, I am leaving you with your thoughts. Carol T From owner-gene-linkage@net.bio.net Wed Aug 12 23:00:00 1998 Path: biosci!news.stanford.edu!newsfeed.berkeley.edu!news.maxwell.syr.edu!newsfeed.nacamar.de!nntp.news.xara.net!xara.net!server5.netnews.ja.net!server3.netnews.ja.net!server4.netnews.ja.net!server2.netnews.ja.net!ral!usenet From: Paul Denny Newsgroups: bionet.molbio.gene-linkage Subject: SSLP allele sizes for CBA vs. BALB strains? Date: Thu, 13 Aug 1998 17:21:45 +0100 Organization: MRC Mouse Genome Centre Lines: 100 Message-ID: <35D31264.89E4C937@har.mrc.ac.uk> NNTP-Posting-Host: 193.63.70.134 Mime-Version: 1.0 Content-Type: multipart/alternative; boundary="------------1D9314DCF9C2065C62F6CF19" X-Mailer: Mozilla 4.05 (Macintosh; I; PPC) --------------1D9314DCF9C2065C62F6CF19 Content-Type: text/plain; charset=us-ascii; x-mac-type="54455854"; x-mac-creator="4D4F5353" Content-Transfer-Encoding: 7bit Is anyone working on crosses involving CBA and BALB/C strains and sizing SSLP markers? We are desperate for polymorphic markers for this strain combination, as they appear quite closely related, with only about 25-30% polymorphism. thanks in advance Paul -- ------------------------------------------------------------------------ Paul Denny Genome Group Leader MRC UK Mouse Genome Centre & Mammalian Genetics Unit Harwell, Oxfordshire OX11 ORD, UK. Phone (direct) +44-(0)1235-824535 Phone (switchboard) +44-(0)1235-834393 Fax +44-(0)1235-824540 email paul@har.mrc.ac.uk Website http://www.mgc.har.mrc.ac.uk --------------1D9314DCF9C2065C62F6CF19 Content-Type: text/html; charset=us-ascii Content-Transfer-Encoding: 7bit Is anyone working on crosses involving CBA and BALB/C strains and sizing SSLP markers? We are desperate for polymorphic markers for this strain combination, as they appear quite closely related, with only about 25-30% polymorphism.

thanks in advance

Paul

-- 

Paul Denny
Genome Group Leader
MRC UK Mouse Genome Centre & Mammalian Genetics Unit
Harwell, Oxfordshire
OX11 ORD, UK.
 
Phone (direct)  +44-(0)1235-824535
Phone (switchboard) +44-(0)1235-834393
Fax +44-(0)1235-824540
email paul@har.mrc.ac.uk
Website http://www.mgc.har.mrc.ac.uk
 

 
 

   --------------1D9314DCF9C2065C62F6CF19-- From owner-gene-linkage@net.bio.net Wed Aug 12 23:00:00 1998 Path: biosci!daresbury!not-for-mail From: 1 Newsgroups: bionet.molbio.gene-linkage Subject: Having any problems with "Perkin Elmer GeneAmp system 2400.?" Date: 13 Aug 1998 07:12:07 +0100 Lines: 24 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <6qu03n$mss@mserv1.dl.ac.uk> X-Authentication-Warning: firewall.barc.ernet.in: smap set sender to using -f MIME-Version: 1.0 Original-To: Newsgroup Bionet , "bionet.molbio.methds-reagnts" Fellow Researchers, We had bought a Perkin elmer Geneamp system 2400 thermal cycler around 1.5 yrs back.It had initially given satisfactory results, but after 7-8 months of working it was erratic in its amplification.Infact It used to give amplification intermitently,which caused loss of 7 months of labour and lots of chemicals till we finally realised that the machine had gone bad. The Indian agents here repaired it, but it has the problem where a couple of wells don't give any amplification. This is not a isolated case.In Mumbai (INDIA) there are 4 pcs of same model with problems. I would like to if there are others in the World who are having problems with the 2400 PCR system especially from underdeveloped countries. Suresh Shettigar email Scientific Officer MB & AD.1-108-H. BARC Mumbai-85 INDIA. Note: Perkin elmer are welcome to respond to this question. From owner-gene-linkage@net.bio.net Fri Aug 14 23:00:00 1998 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.gene-linkage Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 15 Aug 1998 02:00:05 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Distribution: world Message-ID: <199808150900.CAA26308@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-gene-linkage@net.bio.net Wed Aug 19 23:00:00 1998 Path: biosci!news.stanford.edu!newsfeed.berkeley.edu!news.maxwell.syr.edu!nntp.news.xara.net!xara.net!server5.netnews.ja.net!server3.netnews.ja.net!qmw!not-for-mail From: Dave Curtis Newsgroups: bionet.molbio.gene-linkage Subject: Re: disease gene Date: Thu, 20 Aug 1998 18:45:52 +0100 Organization: Queen Mary & Westfield College, London, UK Lines: 20 Message-ID: <35DC60D0.DC869F1C@hgmp.mrc.ac.uk> References: <35DC42B4.2452B7EF@my.signature.file> NNTP-Posting-Host: dialup-port15.qmw.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.05 [en] (Win95; I) > Is that possible a disease locus has several alleles but only one of > these alleles causing disease? Any example or refernece? Very many genes will have harmless polymorphisms, perhaps not even in coding areas or affecting amino acid sequence, and each of these can be viewed as an allele. If there are also one or two harmful mutations then the gene has some alleles causing disease and others not. I would have thought that practically every disease locus fitted these criteria. Of course, if the variation has to be at exactly the same site in terms of coding sequence then the there would be fewer examples. However this is probably unnecessarily restrictive because when multiple different mutations cause disease they generally occur at different places within the gene. From the point of view of the locus these are all alleles. If you go right down to the sequence I suppose you could argue that they represented different polymorphic systems. For practical purposes, a disease locus which can harbour pathogenic mutations will typically also have several harmless polymorphic systems within it or close to it, and these can be used as genetic markers for some applications. From owner-gene-linkage@net.bio.net Wed Aug 19 23:00:00 1998 Path: biosci!news.stanford.edu!newsfeed.berkeley.edu!news.maxwell.syr.edu!cpk-news-hub1.bbnplanet.com!cpk-news-feed4.bbnplanet.com!cpk-news-feed1.bbnplanet.com!news.bbnplanet.com!news.iquest.net!cronkite.d48.lilly.com!not-for-mail From: Chi-Hse Teng Newsgroups: bionet.molbio.gene-linkage Subject: disease gene Date: Thu, 20 Aug 1998 10:37:24 -0500 Organization: Eli Lilly and Company Lines: 13 Message-ID: <35DC42B4.2452B7EF@my.signature.file> NNTP-Posting-Host: oct97ct.d51.lilly.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.04 [en] (Win95; U) Hi, Is that possible a disease locus has several alleles but only one of these alleles causing disease? Any example or refernece? Thanks. Chi-Hse Teng -- Chi-Hse Teng Please use this e-maill address: teng_chi-hse@lilly.com From owner-gene-linkage@net.bio.net Thu Aug 20 23:00:00 1998 Path: biosci!news.stanford.edu!newsfeed.berkeley.edu!howland.erols.net!world6.bellatlantic.net!news From: jaugust@bellatlantic.net (John Augustine) Newsgroups: bionet.molbio.gene-linkage Subject: EXCLUSIVE YOU Date: Fri, 21 Aug 1998 12:49:34 GMT Organization: Bell Atlantic Internet Solutions Lines: 15 Message-ID: <35dd6bfb.434456@news4.bellatlantic.net> NNTP-Posting-Host: client-151-197-124-10.bellatlantic.net X-Newsreader: Forte Free Agent 1.1/16.230 Why do we experience Life from this body and no other body? What is so UNIQUE (One of a Kind) in your body, which is EXCLUSIVE (You)? It does NOT exist in any other body. 5.8 billion bodies with the same "ingredients" except for one. If we had a machine that could make a perfect duplicate of you, atom for atom, would you experience Life from your original body only, or both your original and your duplicate bodies? Identical twins are duplicate bodies and they have identical genes. However, each of the twins experiences Life from his/her own body, and not both bodies. Each body has an EXCLUSIVE One of a Kind "ingredient".  From owner-gene-linkage@net.bio.net Sun Aug 23 23:00:00 1998 Path: biosci!MAIL.VT.EDU!inah From: inah@MAIL.VT.EDU Newsgroups: bionet.molbio.gene-linkage Subject: postdoctoral position Date: 24 Aug 1998 07:04:47 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 59 Sender: daemon@net.bio.net Distribution: world Message-ID: <199808241401.KAA29184@sable.cc.vt.edu> NNTP-Posting-Host: net.bio.net !! POSTDOCTORAL POSITION AVAILABLE !! Organization: Departments of Dairy Science and Statistics, and Interdepartmental Genetics Program, Virginia Tech, Blacksburg Desired start date: immediate Duration: up to 2 years (1-year contract with renewal) Area of research: Statistical Genetics, Statistical Gene Mapping and Genetic Parameter Estimation, Generalized Linear Mixed Models. Specific research areas include multiple polygene mapping in complex pedigrees using maximum likelihood, mixed linear model, and Bayesian methodologies, polygene mapping for categorical traits, statistical issues in fine-mapping of QTLs, application of sampling-based algorithms to genetic parameter estimation, and finite-loci models. There are also increasing opportunities for collaborations with other researchers on campus to perform DNA sequence analyses. Project Leader: Dr. Ina Hoeschele, Professor of Genetics, Adjunct Professor of Statistics Requirements: Ph.D. in Genetics, Biology, Statistics, or related field; computing experience: familiarity with Unix environments, considerable and current programming skills in Fortran90, C or C++; good communication skills Responsibilities: The main responsibility of the position(s) will be to conduct research on a project within the funding framework; additional responsibilities include presentation of research results at scientific meetings and preparation of manuscripts for publication, and may include participation in the advisement of graduate students, and presenting lectures at the graduate level, if desired. Postdoctoral fellows are given the opportunity to audit one course per semester. Salary: $30,000 + benefits The successful candidate will be working in a team with two other postdoctoral fellows. Please send Curriculum Vitae, a list of publications, and three references to: Dr. Ina Hoeschele Department of Dairy Science 2160 Litton Reaves Hall Virginia Tech Blacksburg, VA 24061-0315 U.S.A. If you need additional information, please contact Dr. Ina Hoeschele at: phone: (540) 231-4760, fax: (540) 231-5014, email: inah@vt.edu. Additional information may be found on our homepage (http://www.dasc.vt.edu/ Hoeschele/ hoeschele.html). Ina Hoeschele Professor of Genetics Adjunct Professor of Statistics phone: (540)231-4760 fax: (540)231-5014 email: inah@vt.edu homepage: http://www.dasc.vt.edu/hoeschele/hoeschele.html homepage for VT Genetics Program: http://www.dasc.vt.edu/hoeschele/genetics.htm homepage for VT Institute for Genomics: http://www.nbiap.vt.edu/vigen From owner-gene-linkage@net.bio.net Mon Aug 24 23:00:00 1998 Path: biosci!news.stanford.edu!newsfeed.berkeley.edu!howland.erols.net!feed2.news.erols.com!erols!newsfeed.xcom.net!shore!news.ultranet.com!not-for-mail From: "Marc Andelman" Newsgroups: bionet.molbio.gene-linkage Subject: San Francisco job Date: Tue, 25 Aug 1998 16:48:03 -0700 Organization: UltraNet Communications , an RCN Company http://www.ultranet.com/ Lines: 27 Message-ID: <6rv7ff$5sb$1@strato.ultra.net> NNTP-Posting-Host: d16.dial-4.cmb.ma.ultra.net X-Complaints-To: abuse@ultra.net X-Ultra-Time: 25 Aug 1998 20:40:15 GMT X-Newsreader: Microsoft Outlook Express 4.72.2106.4 X-MimeOLE: Produced By Microsoft MimeOLE V4.72.2106.4 Molecular Biologist wanted Industrial experience requested. This is for an early stage start up in the San Francisco area backed by a top notch venture firm. This person will utilize homologous PCR, gene databases, and other techniques to identify a family of cell phenotype specific proteins important to fundamental cell processes. Must have good managerial ability to be able to manage an important outside collaboration. Interested people please E mail Marc Andelman, President, Biosource Inc. drgonfly@ultranet.com, or call 508 853 8803 or fax 508 853 8772. Biosource is an employer paid fee service. Unless specifically requested, please allow me only to get back in touch if this or some other position is a dead ringer for each parties interests. I will of course call first before sending anyone's information along. Regards, Marc Andelman From owner-gene-linkage@net.bio.net Mon Aug 24 23:00:00 1998 Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed4.bbnplanet.com!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newspeer.monmouth.com!news.ultranet.com!not-for-mail From: "Marc Andelman" Newsgroups: bionet.molbio.gene-linkage Subject: genomics position Date: Tue, 25 Aug 1998 16:53:17 -0700 Organization: UltraNet Communications , an RCN Company http://www.ultranet.com/ Lines: 20 Message-ID: <6rv7p8$2r8$1@ligarius.ultra.net> NNTP-Posting-Host: d16.dial-4.cmb.ma.ultra.net X-Complaints-To: abuse@ultra.net X-Ultra-Time: 25 Aug 1998 20:45:28 GMT X-Newsreader: Microsoft Outlook Express 4.72.2106.4 X-MimeOLE: Produced By Microsoft MimeOLE V4.72.2106.4 Biosource is the oldest recruitment firm in the biotech industry and an employer paid fee service. We currently have a client seeking to hire people to manage positional cloning projects. Experience such as with BACs, YACs, and contigs is required. This is an extremely well funded company outside of the United States. Interested people please E-mail Marc Andelman at drgonfly@ultranet.com, or call 508 853 8803, or FAX 508 853 8773. Unless specifically asked, please allow me only to get back in touch if this or some other position is a dead ringer for a person's and the client's interest. I will of course call first before sending anyone's information along. Thank you, Marc Andelman From owner-gene-linkage@net.bio.net Fri Aug 28 23:00:00 1998 Path: biosci!news.stanford.edu!nntp.cs.ubc.ca!unixg.ubc.ca!msw From: msw@unixg.ubc.ca (mickey) Newsgroups: bionet.molbio.gene-linkage Subject: systat and cluster analysis Date: 29 Aug 1998 08:13:08 GMT Organization: University of British Columbia, Vancouver, B.C., Canada Lines: 7 Message-ID: <6s8d6k$ott$1@nntp.ucs.ubc.ca> NNTP-Posting-Host: netinfo3.ubc.ca X-Newsreader: TIN [version 1.2 PL2] has anyone ever used systat for qualitative cluster analysis? and if so, how did you account for missing data in the matrix? whenever i've used it, systat has automatically discarded any sample with missing values, rendering most of my analysis useless. thanks in advance mick From owner-gene-linkage@net.bio.net Sun Aug 30 23:00:00 1998 Path: biosci!MAIL.VT.EDU!inah From: inah@MAIL.VT.EDU Newsgroups: bionet.molbio.gene-linkage Subject: (Fwd) stat gen position Date: 31 Aug 1998 12:59:07 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 49 Sender: daemon@net.bio.net Distribution: world Message-ID: <199808311956.PAA00928@sable.cc.vt.edu> NNTP-Posting-Host: net.bio.net This message was sent to me by Cathy Laurie of Cereon Genomics. They are looking for a statistical geneticist. ------- Forwarded Message Follows ------- From: CATHY.C.LAURIE@cereon.com To: inah Subject: stat gen query Date: 04 Aug 1998 09:40:18 -0500 Dear Ina, I am writing to ask your advice in identifying potential job candidates for a position in statistical genetics, but first a little background information. I recently moved from Duke University to become director of genetics at a biotechnology company in Cambridge, Mass called Cereon Genomics. Cereon is a collaboration between Millennium Pharmaceuticals (a high tech genomics company) and Monsanto. The goal of the company is to develop and apply genomics to agricultural problems. We are mainly concerned with microbial and plant genetics. The company is founded by a group of scientists, many from academia, and it provides some exciting opportunities to pursue interesting biological questions that require large resources. One of the areas of application is identification and cloning of QTLs from crop plants and I am organizing a company-wide effort to do this. We need a statistical geneticist to work primarily on QTL analysis, but also on other statistical problems in genomics (e.g. mapping, transcriptional profiling). I would like to find someone with really good training in statistics who understands genetics, can think critically, and be adaptable. Direct experience in QTL work is highly desirable, but not essential. Someone with at least a few years of postdoctoral experience is most desirable. Please let me know if any potential candidates come to mind or if you can recommend other people to whom I should send this query. Thank you, Cathy Ina Hoeschele Professor of Genetics Adjunct Professor of Statistics phone: (540)231-4760 fax: (540)231-5014 email: inah@vt.edu homepage: http://www.dasc.vt.edu/hoeschele/hoeschele.html homepage for VT Genetics Program: http://www.dasc.vt.edu/hoeschele/genetics.htm homepage for VT Institute for Genomics: http://www.nbiap.vt.edu/vigen From owner-gene-linkage@net.bio.net Mon Aug 31 23:00:00 1998 Newsgroups: alt.support.cancer,bionet.molbio.gene-linkage Path: biosci!news.stanford.edu!newsfeed.concentric.net!newshub.northeast.verio.net!news2.ais.net!jamie!ais.net!uunet!uunet!uunet!in3.uu.net!world!sphinx From: sphinx@world.std.com (SPHINX Technologies) Subject: Re: All lies? Questions on conventional cancer theraphy. Message-ID: Date: Tue, 1 Sep 1998 08:42:16 GMT Bcc: sphinx References: <6sbg8c$hi9$1@apple.news.easynet.net> <1998083020453100.QAA13883@ladder01.news.aol.com> Organization: SPHINX Technologies, Inc., Wellesley Hills, MA Keywords: cancer, pleomorphic bacteria, gene sequencing, drug discovery, hints Lines: 316 (molbio readers please bear with me, or string-search for "genome" or "Nobel" :^) In article <1998083020453100.QAA13883@ladder01.news.aol.com>, AJKim0 wrote: >"Ozgur Karabiyik" wrote: >>I have been told that the conventional cancer treatment is full of lies and >>driven by a combination of money hungry scientists and the blind minded >>doctors. I don't think scientists in general are "money hungry", nor are doctors in general greedy. If anybody in the field is motivated by greed, it would be the Big Money Interests behind the drug companies. The scientists and doctors, like anybody else, like to be well paid for the long years of effort they put in learning their skills. The businesspeople and the stockholders want to see a good "return on investment" to induce them to put their money into drug and medical investments rather than the bank. There's nothing wrong with any of this. As for lies... that would be another matter, if true. Deliberately suppressing information about better treatment methods because they would amount to a quick and inexpensive cure, and cut off the flow of profits on the more expensive medicines... THAT would be an abomination. But it wouldn't involve the doctors or the scientists. Maybe some business executives of the type that brought us the Big Tobacco conspiracy to hide information on addictiveness of nicotine and on manipulation of nicotine levels to ensure addiction, but not the scientists and doctors. >You have been told this? By whom? Admittedly, apart from surgery (because you >can cut it right out), there is quite a bit of uncertainty when it comes to >[mainstream cancer treatments]? >[But there is no clinical data on alternative treatments showing any >effectiveness.]? < snipped t this too, approximate reconstruction. > I agree, although proponents of alternative cancer therapy have pointed out some disturbing discrepancies between the final verdict on some of those studies and the results of other earlier studies. And they have made criticisms of the methodology of the studies which have, time after time, dismissed herbal and other "alternative" cancer treatments or cures as "ineffective". I feel that cancer patients deserve to be made aware of this other side of the controversy. There is one book I've identified that I feel does a fairly good job of that, and it's: [1] Ross Pelton, R.Ph., Ph.D., and Lee Overholser, Ph.D., "Alternatives in Cancer Therapy", NY: Fireside/Simon&Schuster, 1994. ISBN 0-671-79623-2. >>Is there a truth in this? Ask yourself why the number of people who are >>diagnosed with cancer does not drop over the years while the money reserved >>for cancer resarch is rising. Somebody is getting rich behind this scheme. >The fact that we still have lots of people newly diagnosed with cancer has >nothing to do with the TREATMENT of cancer. There may be a number of causative >factors: increased fat intake, increased amount of toxins in the environment, >people living longer, etc. And increased _hydrogenated_ fat intake (aka trans-fatty acids), increased amount of preservatives and pesticide residues in food, REDUCED intake of ESSENTIAL fatty acids, notably those containing omega-3 oils; thinning of the ozone layer; increased exposure to ionizing (nuclear) radiation; increased exposure to non-ionizing radiation (radio waves). Mineral depletion of soils leading to mineral deficiencies. Maybe increased exposure to synthetic chemicals in clothing (synthetic fibers next to the skin etc.). >But the number of newly diagnosed cases and death rates declined in the last >several years: >"Cancer incidence and death rates for all cancers combined and for most of the >top 10 cancer sites declined between 1990 and 1995, reversing an almost 20-year >trend of increasing cancer cases and deaths in the United States. A scientific >paper on the topic, "Cancer Incidence and Mortality, 1973-1995" was published >in the March 15, 1998 issue of CANCER." That's good news - this might reflect the acknowledgement, just around 1990, that antioxidants really ARE important, as those "health food nuts" and "alternative medicine" nuts had been saying for 25 years or more by then! >The money which is allocated to cancer research is increasing because there is >general awareness of the fact that we aren't winning the war against cancer >despite years of research, and that we should tackle this problem with more >fervor than we have been. Who's getting rich? Probably the pharmaceutical >companies which manufacture and market the chemotherapeutic drugs, simply >because their products are "in demand." Yep. Although some of the "alternative medicine" folks are probably doing quite well too, since the public has started to take its own path and use these alternatives almost more than it does "conventional medicine" for some matters, usually chronic, non-life-threatening illness where they have time to see for themselves the effectiveness of the methods without great risk. In many of those kinds of cases, evidently they are finding out that the people who are selling natural remedies know what they are talking about, and deciding that those kinds of remedies are more cost-effective in meeting their needs. And this, even when these remedies aren't covered by their medical insurance! >>Why a cure still not been found? According to Dr. Robert C. Atkins, a "complementary" medicine practitioner (="alternative" PLUS "traditional" -- whatever he believes is best for each patient), not one but SEVERAL of them HAVE been found, but are being kept from the public by labelling them "unproven". See, for example, his comments in his book "Dr. Atkins' Health Revolution". His Web site is at www.atkinscenter.com, and his toll-free phone number is 1-800-2ATKINS. I have no financial interest in any of this, by the way - I just think it is relevant and that Dr. Atkins is both competent and sincere in what he does. And according to Dr. Lorraine Day, SHE cured herself of advanced (breast) cancer, and others can, too, using methods which I think are along the lines of the "macrobiotic diet" approach [1], though probably with her own twist on it. Her Web site is at www.drday.com, toll-free phone is 1-800-574-2437. She offers two videotapes describing her methods, I believe, in enough detail that a cancer patient could put them into practice without having to use her as their attending physician. I also have no financial interest in Dr. Day's operations. I have a *LOT* of _intellectual_ interest in her thesis that "you only get well by rebuilding your immune system". Even if you use Mainstream Medicine methods initially, that advice makes sense to prevent a recurrence of disease for the long term. >Remember, the human body comprises trillions of cells. And each one of these >cells has genome containing hundreds of thousands of genes, all controlled by >exquisitely intricate regulation mechanisms. Mutations in the genes which >allow normal growth of cells (proto-oncogenes), or the genes which normally >inhibit growth (tumor suppressor genes), will allow cancer cells to get out of >hand. We're talking about things at the molecular level. Do you think it's >easy to spot which genes have mutated, among the hundreds of thousands? Even >our body's own immune system has trouble differentiating between normal and >cancer cells. This raises an interesting point. If this is truly the mechanism of cancer, shouldn't there be some obvious patterns that arise from the combinatorial possibilities of the genome? How many different kinds of mutations of each gene would be possible? A lot, right? How many of them would be "viable" in the sense of causing cancer? Would there not be a huge variety of effects seen? What are there, 64 "letters" in the alphabet of nucleotides, of which twenty are codons for amino-acid residues, and a few others are stop codes and whatnot? With 150,000 or so codons in one gene, there ought to be a MIND-BOGGLING number of possible mutations, and all of them ought to have an easily-calculated probability. To me, it seems likely that there ought to be a gazillion different ways that mutations could cause growth to get out of hand and be observed as cancer, even if that gazillion is a tiny fraction of all possible mutations. OTOH if Glover, Rife, Slifkin, Avecedo, Livingston-Wheeler, et al are right, and there is ONE cancer organism, the number of variations of cancer would basically come down to the number of different types of cell lines that can be infected. I.e., we don't see astronomically large numbers of different kinds of cancers, we see the same kind recurring in all humans and all animals, dependent primarily on what site in the body is infected. I'm not very close to the research on the genetics of cancer, but from what little I DO know, the combinatorics strongly suggest that these folks are right and the mainstream is self-deluded. I would almost go so far as to say that this is OBVIOUS, once you pose the question in the way I just did. Any comments from the peanut gallery out there? (Of course this argument doesn't even USE the fact that the above-mentioned scientists supposedly could isolate the organism from and cancerous cell and show it to you in a microscope [2] [3].) >>Is it because the conventional theraphy is >>the wrong path to fight against cancer? THAT, I'm afraid, is a decision each patient must make for himself or herself. >I wouldn't say "wrong," but it's an unrefined approach, because conventional >therapies attack both the normal and the cancerous cells, although they mainly >target rapidly dividing cells. That's right, whereas if the "single cancer organism" theory is right, then the folks who are trying to come up with cancer cures are barking up the wrong tree. Which could be the REAL reason that cures are proving elusive. Maybe the genomists should go find out what this organism IS that the other school says they can REPEATBLY ISOLATE from every cancerous cell [1], sequence its genome, and design a drug to kill *IT*. What's the big deal? If I *WERE* in that field, that's sure as Heck what *I* would be doing in pursuit of my Nobel Prize. (just trying to MOTIVATE somebody out there listening.) >>Why do we, people diagnosed with >>cancer, have to shut our eyes and take in what ever our doctors are offering >>us as the treatment? I had to. > >We don't have to, but I did, like you did, because I did have faith in >conventional therapies. I looked at the medical literature, and found that the >chemotherapy drugs and the extensive radiation that I received were effective >against my cancer, compared to radiotherapy given alone. > >The other options would have been for me to go up to 1) prayer mountain, and >pray for healing, or 2) look for some other venues of treatment. The reasons >that I did neither of these are that 1) although I have faith in God, I >believed that He wanted me to give it my best shot first, and 2) there wasn't >enough data out there for me to try alternative therapies as the mainstay of >treatment. During my chemo and radiation, at the urging of my sister, I took a >whole lot of Reishi mushroom (bitter as heck) and ginseng extracts, as well as >antioxidants. But my cancer spread anyway. More's the pity you didn't look into reference [1] above and find out more about alternative treatments that REALLY work. I'm not saying you should have totally RELIED on such treatments, but IMO you could have found a half dozen or so treatments with a lot more data on them than the ones you mentioned. (I've never heard of either of them as a cancer treatment, by the way, for whatever that's worth. Except that now I see from a quick look in the Index of [1] that ginseng is mentioned, and the passage where it appears says that the active ingredient in it as far as cancer is concerned is probably Geranium-132, to which a whole chapter of the book is devoted. This is apparently not an isotope but an organically-bound form of Geranium. The chapter suggests that it's an immune-system booster, BTW. Also, ginseng (Japanese variety) is listed as having 260-320 ppm of Ge-132, presumably by weight. And the clinical trials which showed useful positive results used 50 mg/Kg/day of Ge-132. Presumably you can do the math to compare this with how much you got from ginseng.) Then there's "The Grape Cure", described in detail in [4], and Essiac, about which I don't know very much at the moment except that a lot of people credit it for ridding them of cancer. I suppose I should order Caisse's book on it. I've seen a posting recently stating that several different "recipes" for Essiac Tea are available in various places, and some of them are bogus, i.e. ineffective. I could probably find the details in my "vast, well-organized file system". Or you could use DejaNews. >They told me that my condition was at a critical stage and I could die >due to the size of the tumor. And they assured me that this was the >only way to fight cancer. Were they wrong? I don't know. There's >probably something out there somewhere that will help shrink the size of >the tumor, but how would you be able to find out? As far as they were >concerned, that WAS the only (effective) way to fight cancer. > >Maybe, in the short term, it is true. The chemotheraphy and radiotheraphy >helped me greatly. But I know, as much as my doctors, that the tumor WILL >grow back. This sounds like an ideal situation for you to take a renewed interest in alternative therapy methods that just MIGHT prevent that outcome, since you presumably have some time, and nothing better to do in the meantime. If I were in that situation, I'd develop a real strong taste for GRAPES [4] for awhile, and probably for FLAX OIL too. As a minimum. If I were motivated by being PERSONALLY in that situation, I'd probably do a lot more detailed evaluation of the information in [1] and maybe personally talk to a few complementary practitioners such as Day and Atkins, before deciding what "alternative" path to follow. But please note that this constitutes merely a free-speech expression of my opinion, not advice. >Did you go into remission? If you did, how do you know that it WILL grow back? >For my cancer, 66% of people who received the chemo and radiation that I did >never experience recurrence. You could very well fall into the group of people >who are cured (or at least 5 years without recurrence). >>All I can say is there are people >>who get better without the use of conventional treatment. > >I believe so. But I wonder how likely it would be for the majority of cancer >patients. Well, you could look at some of the statistics in [1], and make the assumption that they are for real. And you could (particularly with your evidently very high level of medical knowledge) make some informed choices of which methods ought to combine well. >>And I am looking for those alternatives, too. > >Best of luck to you. Right now I'm thinking about enrolling in a clinical >trial, because for me, I think it holds more promise than Essiac tea, laetrile, >hydrazine sulfate, or anything else. It's my opinion based on my own research. Unless you're interested in purity of experimental methodology, instead of maximizing your own chances, why not do that IN ADDITION TO some alternative approaches, particularly ones that are mainly nutritional and aimed at building general health? And if you want some guidance in drug-nutrient interaction, I can vector you over to a world-class consultant in that area. E-mail me if interested. >>Search www for IAT Center, Gaston Naessens Clinic, Livingston Clinic. >Was all that a plug for these clinics? If it was, reference [1] gives some background on all of them, for anybody who's interested. >>Don`t be affraid of cancer, be affraid of the wrong threatment! That's what Dr. Lorraine Day says, also, in her video "I'm Not Afraid of Cancer Anymore!". (Which by the way, I've never seen. But based on an interview which I heard on a talk show, I would expect her videos to be worth their $20 price tag for anyone who is evaluating alternative methods.) >I'm not afraid of cancer. I'm afraid that there are quacks and scammers out >there who are willing to make a quick buck off a desperate cancer patient >without hesitation. >Andre Alas, that's probably true. But there are also people out there who are apparently competent and sincere, and have identified which methods REALLY work. You really ought to read, for example, Atkins's "Health Revolution" book if you haven't already. -John S. [1] See above, near beginning of posting. [2] Barry Lynes, "The Cancer Cure That Worked: Fifty Years of Suppression", Queensville, Ontario: Marcus Books, 1987; Fourth printing, 1992. ISBN 0-919951-30-9. [3] Dr. Robert E. Netterberg and Robert T. Taylor, "The Cancer Conspiracy", NY: Pinnacle Books, Inc., 1981. ISBN 0-523-41466-8. [4] Johanna Brandt, "The Grape Cure", reprinted by Ehert Literature Publishing Co., Inc., 19 Babcock Place, Yonkers, NY 10701-2714. I bought my copy at a local "natural-foods" supermarket, possibly Bread & Circus or Nature's Heartland (Boston are). Note: B&C is known as "Fresh Fields" in the Washington DC area after recently acquiring FF. From owner-gene-linkage@net.bio.net Mon Aug 31 23:00:00 1998 Path: biosci!AAMERICAFIRSTFINANCIAL.COM!finance From: finance@AAMERICAFIRSTFINANCIAL.COM Newsgroups: bionet.molbio.gene-linkage Subject: AD: Visa - MasterCard - 100% Guarantee Regardless of Credit Problems Date: 1 Sep 1998 12:28:03 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Distribution: world Message-ID: <199809011924.PAA05000@adni.net> NNTP-Posting-Host: net.bio.net This message is sent in compliance of the new e-mail bill: SECTION 301, Paragraph (a)(2)(C) of s. 1618 Sender : America First Financial, 15615 Waterloo Road, Cleveland, OH 44110 Phone : 216-481-3573 E-mail : finance@aamericafirstfinancial.com To be removed from our mailing list, simply reply with "REMOVE" in the subject. Welcome to America First Financial, your credit card connection. 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