From owner-gdb@net.bio.net Wed May 08 23:00:00 1996 Newsgroups: bionet.molbio.embldatabank,bionet.molbio.gdb,bionet.molbio.evolution,bionet.molbio.genbank Path: biosci!rutgers!uwm.edu!lll-winken.llnl.gov!nntp.coast.net!howland.reston.ans.net!vixen.cso.uiuc.edu!newsfeed.internetmci.com!in2.uu.net!sangam!iitb!powai!powai.cc.iitb.ernet.in!mmrajan From: "Dr.M.M.Rajan" Subject: Mosquito Cell lines Sender: news@powai.cc.iitb.ernet.in (news system user) Message-ID: Date: Thu, 9 May 1996 12:52:47 GMT To: cell@nccs.ernet.in Reply-To: "Dr.M.M.Rajan" Content-Type: TEXT/PLAIN; charset=US-ASCII Mime-Version: 1.0 Organization: Computer Centre, Indian Institute of Technology, Bombay Lines: 44 Xref: biosci bionet.molbio.embldatabank:637 bionet.molbio.gdb:458 bionet.molbio.evolution:4528 bionet.molbio.genbank:2288 >From mmrajan@cc.iitb.ernet.in Thu May 9 18:03:03 1996 Date: Thu, 9 May 1996 17:58:02 +0530 From: "Dr.M.M.Rajan" Newsgroups: bionet.cellbiol Subject: Mosquito cell lines! Dear Newsgroup reader, Hi! One of my colleagues, Dr.Supriya Kshirsagar, in the Molecular Biology Division is working on mitochondrial dna. She would like to know where she could get mosquito genomic library of any of the species (of the genera Anopheles, Aedes, Culex) any stage (larvae to adult). Anybody with knowledge or information about this, may I please request them to write back or contact: cell@nccs.ernet.in Thanking in anticipation, Yours M.M.Rajan. ---------------------------------------------------------------------------- Dr.M.M.Rajan, M.B.,B.S., Doctoral Research Scholar - Biomedical Engineering, School of Biomedical Engineering, Indian Institute of Technology, Bombay - 400 076. email : mmrajan@cc.iitb.ernet.in At present at: National centre for Cell Sciences Pune - 411 029 cell@nccs.ernet.in ----------------------------------------------------------------------------- From owner-gdb@net.bio.net Sun May 12 23:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.gdb Subject: IMPORTANT - BIOSCI Fundraising Update! Date: 13 May 1996 02:01:21 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 154 Sender: daemon@net.bio.net Distribution: world Message-ID: <199605130900.CAA27944@net.bio.net> NNTP-Posting-Host: net.bio.net BIOSCI is about halfway to its funding goal!! I'm interrupting the usual monthly posting of the BIOSCI miniFAQ to bring you up to date on BIOSCI fundraising progress, a topic of concern to your future use of this resource. Thank you in advance for taking the time to read this message carefully. Last year we announced that BIOSCI was going to adopt the U.S. Public Broadcasting System model to fund its operations after our DOE/NSF grant runs out later this year. Unlike PBS, we are not soliciting contributions from users; we are only selling ads on our Web pages solely to cover our operating costs. Our goal is to seek sponsorships until we build up an operating reserve of about $100,000 and then cease further promotions until we need to build the reserve back up. (The accountants among our readership will be familiar with the problem of deferred revenue which we can not safely utilize until ads have been displayed for a period of time.) We are only about halfway to our funding goal and need to raise further funds to avoid having to curtail services at net.bio.net. Fundraising is time-consuming, however, and we need your help as explained further below. Our operating costs consist of our network connection, phone lines, hardware maintenance (we will be getting newer and faster hardware soon!), plus 0.7 FTE of salaries covering UNIX systems admin, technical support, quality assurance, i.e., testing, of our system, and administrative costs (such as the time it takes to actually find/write/call potential sponsors and raise money!). Although the BIOSCI staff does get compensated for a portion of the work that they do, this project has always received a lot of free after-hours and "vacation" time labor, so we hope that no one will begrudge the time that we do charge to the project to serve you. All of the three part-time staff members, Dave Mack, Julie Lawrence, and myself, have full time day jobs and families in addition to working hard to keep this service running for all of you. Julie and Dave Mack are subcontractors for BIOSCI; my time that is charged to the project defrays a portion of my regular salary instead of adding to my income. Besides having to relocate the project, we were very busy this last year building new infrastructure such as our WWW hypermail interface to the system. This was released last December along with scores of WAIS indices for the newsgroups. Virtually everything is complete, although we do continue to find and fix bugs (many through your helpful feedback!). We are still having some problems with our WAIS indexing. The archives continue to grow rapidly. We are running over 100 indexes now versus three previously and any systems crashes cause greater havoc with the indexing than before! We are still working to fix this as fast as our resources permit and appreciate your patience, but we have been able to automate a lot of the infrastructure to reduce labor as compared to past requirements. We have also implemented new software to make moderation of BIOSCI/bionet newsgroups much easier and combat the growing problem of Internet junk mail and USENET "spamming." About 20% of our groups are now moderated, many of them by the BIOSCI staff! This, for example, made a major difference last year in the quality of content in our EMPLOYMENT/bionet.jobs.offered newsgroup which many commercial concerns and recruiting firms are using **without charge** to recruit candidates for positions in the biological sciences. We are also now in a position to have sponsors for individual newsgroups as you will have noticed if you have visited http://www.bio.net/ and clicked on "Access the BIOSCI/bionet newsgroups" recently. So, how can you help?? ---------------------- As noted above it can take a lot of time to contact potential sponsors if I have to do it all myself. Our request is quite simple. You can do two important things which will take very little time for you individually. First, please use our WWW system at http://www.bio.net/ to access the archives. You can now post or reply to messages via your Web browser. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. Our hope is to quickly raise several large corporate/institutional sponsors on our heavily-used WWW locations (some stats appended below), and then end this sponsorship campaign so that our resources can continue to be used for service provision, not fundraising. Many of our specialty newsgroup WWW archives are still used by small communities of scientists (and they haven't been heavily promoted yet). While these may be valuable niche markets to some advertisers, it will generate more labor and overhead having to find these sponsors, fairly price the locations, and deal with lots of smaller sponsorships than fewer mid-to large sponsors. We are striving to keep our operation as lean and efficient as possible since we are not trying to make careers out of running BIOSCI. We are trying if at all possible to avoid the administrative overhead entailed with processing lots of small payments to reach our fundraising goals. I'd like to thank all of you for your help in advance. In helping us, you are also helping yourselves, not only in keeping this resource available for all of the both large and small research communities that we serve, but also by alleviating the need for us to go back and compete with researchers for tight grant dollars! We promised NSF when we were awarded the BIOSCI grant that we would carry out this mission to make the service self-supporting. With your help, we will succeed in continuing BIOSCI's work into its second decade. Thank you very much! Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net A list of our prime WWW sponsorship locations follow. Please contact us for further details. ---------------------------------------------------------------------- The overall BIOSCI WWW pages are currently visited by users from close to 5500 unique computer hosts per week. Web servers only log the Internet computer/host name and frequently more than one individual can connect to us from a particular host. Main home page, http://www.bio.net, visited recently by about 2100 unique hosts per week Main Newsgroups archives page, http://www.bio.net/archives.html, visited recently by about 1200 Unique hosts per week BIO-JOURNALS archive page, http://www.bio.net/BIO-JOURNALS.html, visited recently by about 1000 unique hosts per week. EMPLOYMENT archive pages: http://www.bio.net:80/hypermail/EMPLOYMENT/ and monthly header pages, visited recently by about 800 unique hosts per week. Address database search page, http://www.bio.net/addrsearch.html, visited recently by about 450 unique hosts per week. Methods newsgroup archive pages, http://www.bio.net:80/hypermail/METHDS- REAGNTS/ and monthly header pages, visited recently by about 350 unique hosts per week. Ads can also be displayed on various combinations of other BIOSCI/bionet newsgroups. Please contact us at biosci-help@net.bio.net for details. ---------------------------------------------------------------------- From owner-gdb@net.bio.net Mon May 13 23:00:00 1996 Path: biosci!GDB.ORG!avoltz From: avoltz@GDB.ORG (Amy Voltz) Newsgroups: bionet.molbio.gdb Subject: bcl2 cDNA in GDB Date: 14 May 1996 09:14:58 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 50 Sender: daemon@net.bio.net Distribution: world Message-ID: <3198B0B8.2A64@gdb.org> NNTP-Posting-Host: net.bio.net >Dear Jeff There is info. on a cDNA probe for BCL2 in the human Genome Database (http://gdbwww.gdb.org) Access the "Finding Genes in GDB" interface at http://gdbwww.gdb.org/gdb5.6/lite/docs/home.html (NB this interface is being redeveloped for the newest release of GDB and will be available shortly - but the data for this clone has not changed.) Choose "Reagents for gene analysis" Enter BCL2 and choose cDNA clones To view detailed info for this clone, which hybridizes to the 3' end of exon 1, choose the highlighted GDB ID. This detail also has contact info. for Yoshihide Tsujimoto in Phila., who submitted this info. to GDB. Please let me know if you have any trouble accessing this information. Sincerely, Amy Voltz avoltz@gdb.org > > Subject: bcl-2 cDNA probe > > Date: Mon, 6 May 1996 13:59:33 GMT > > I am looking for a specific probe for human bcl-2. Does anyone have > this > probe or know of its availability commmericially? > > thanks. > > -- > Jeffrey S. Upperman,MD Internet: upperman@umdnj.edu > Research Fellow Voice: (201) 982-4474 > Dept. Anatomy, Cell Biology & Injury Fax: (201) 982-6803 > Dept. of Surgery Beeper: (201) 708-4150 > New Jersey Medical School Office: (201) 982-5045 > 185 S. Orange Ave., G606 Lab: (201) 982-5404 > Newark, NJ 07103 > > ---------------------------------------------------------------------- > > * Next message: Mike Rogers: "Re: Ni-NTA Spin kit ....HELP" > * Previous message: Robert G. Hamilton: "Re: license for TAQ" > * Next in thread: Richard Moldwin: "Re: bcl-2 cDNA probe" > * Reply: Richard Moldwin: "Re: bcl-2 cDNA probe" From owner-gdb@net.bio.net Mon May 13 23:00:00 1996 Path: biosci!GDB.ORG!avoltz From: avoltz@GDB.ORG (Amy Voltz) Newsgroups: bionet.molbio.gdb Subject: Human DHFR clones in GDB Date: 14 May 1996 09:26:35 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 61 Sender: daemon@net.bio.net Distribution: world Message-ID: <3198B366.1081@gdb.org> NNTP-Posting-Host: net.bio.net > Human DHFR clones? > > Fourie Joubert (fourie@crimson) > 7 May 1996 10:53:27 GMT > > * Messages sorted by: [ date ][ thread ][ subject ][ author ] > * Next message: Cambridge Healthtech Institute: "Meeting > Announcement: Gene Amplification and Detection" > * Previous message: Song Tan: "Re: Inoue Comp Cells" > > ---------------------------------------------------------------------- > > To: methods@net.bio.net > Dear Fourie, The human Genome Database (GDB, http://gdbwww.gdb.org) has information on 10 human genomic clones for the DHFR gene. Access the "Finding Genes in GDB" interface at http://gdbwww.gdb.org/gdb5.6/lite/docs/home.html (NB this interface is currently being redeveloped for the most recent release of GDB and will be available soon, but data for the DHFR gene has not changed.) Choose "Reagents for Gene analysis" Enter DHFR and choose genomic clones. To view detailed information for each clone, including who you can contact to obtain it, choose the highlighted GDB ID. I hope this information is helpful to you. Sincerely, Amy Voltz avoltz@gdb.org > From: fourie@crimson (Fourie Joubert) > > Subject: Human DHFR clones? > > Date: 7 May 1996 10:53:27 GMT > > Hi > > I am trying to obtain clones of human DHFR in any vector. If anyone > would be willing to share, please let me know. > > We are working on the malaria form of the enzyme and would like to > do some comparisons... > > Any help would be sincerely appreciated! > > -------------- > Fourie Joubert > Department of Biochemistry > University of Pretoria > fourie@scientia.up.ac.za > http://suntiger.ee.up.ac.za/biodocs/home.html > +27-12-420-2011 > ---------------------------------------------------------------------- > > * Next message: Cambridge Healthtech Institute: "Meeting > Announcement: Gene Amplification and Detection" > * Previous message: Song Tan: "Re: Inoue Comp Cells" From owner-gdb@net.bio.net Tue May 14 23:00:00 1996 Path: biosci!GDB.ORG!avoltz From: avoltz@GDB.ORG (Amy Voltz) Newsgroups: bionet.molbio.gdb Subject: SWISS-PROT funding crisis, help us ! Date: 15 May 1996 10:53:48 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net ******************************************** http://expasy.hcuge.ch/sprot/help-sprot.html ******************************************** Attention, researchers! If you use SWISS-PROT and want to keep using it, check out the URL given above and respond to the request for support. From owner-gdb@net.bio.net Wed May 15 23:00:00 1996 Path: biosci!GDB.ORG!avoltz From: avoltz@GDB.ORG (Amy Voltz) Newsgroups: bionet.molbio.gdb Subject: EC numbers in GDB Date: 16 May 1996 10:15:40 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 29 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net How can I find the EC (Enzyme Commision) number for my favorite gene in GDB? Are these linked directly to the EC database? ------------------------------------------------------------------------ EC numbers are now linked to the appropriate protein products of genes in GDB. To query for a specific EC number: Search by Query Forms from the GDB homepage. Choose "Enzyme Link" under "External Link" Enter your EC number of choice using this exact format EC: 1.15.1.1 as Name for the Enzyme Link Query Choose Protein SUPEROXIDE DISMUTASE This will retrieve the protein product SUPEROXIDE DISMUTASE Within the detail for this protein are the 3 sod genes, these genes can be accessed directly via these links. To get to an EC number and the EC database from a gene: Choose Product from the gene detail for any gene of interest Only those genes that code for enzyme products will have a product field in the gene detail. This link will take you directly to the protein entry in GDB from which you will see the EC number and link to the EC database. I hope this information is helpful to you. Sincerely, Amy Voltz avoltz@gdb.org From owner-gdb@net.bio.net Wed May 29 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!newshub.csu.net!csulb.edu!drivel.ics.uci.edu!news.service.uci.edu!design.eng.uci.edu!pecota From: Doug Pecota Newsgroups: bionet.molbio.gdb,bionet.general Subject: Best mRNA purification kits for E. coli Date: Thu, 30 May 1996 11:26:32 -0700 Organization: University of California, Irvine Lines: 15 Message-ID: NNTP-Posting-Host: design.eng.uci.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Xref: biosci bionet.molbio.gdb:468 bionet.general:21907 I plan to purify the mRNA for my favorite gene locus in E. coli. It has several different mRNA species 70, 230, 370, 415 and 440 nucleotides long. What is the best RNA kit to purify mRNA of this size? I know several venders sell such kits (Gibco BRL, Clonetech, Qiagen and Boehringer Mannheim). Someone has indicated to me that the Qiagen kit was not very good. If you have experience with these types of kits and could give me a recommendation please contact me or post. Thanks Doug Pecota pecota@eng.uci.edu University of California Irvine