From owner-immunology@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!agate!howland.reston.ans.net!vixen.cso.uiuc.edu!sdd.hp.com!saimiri.primate.wisc.edu!nntp.msstate.edu!tpm-sprl!jbh From: jbh@anat.UMSMED.EDU (James B. Hutchins) Newsgroups: bionet.immunology Subject: Re: Fading problem with flourecein labelling Message-ID: Date: 2 Dec 93 01:44:03 GMT Organization: Dept. of Anatomy, Univ. of Mississippi Medical Center Lines: 29 In article <2div26$6hv@netnews.upenn.edu> cychan@mail.sas.upenn.edu (Cecilia Y Chan) writes: > >I am having a lot of problems with fading using flourescein label for Ab. >I have tried various mounting reagents, but they all seem to fade [stuff deleted] You do not say what you have tried, so excuse me if this is redundant... We have had good luck with three formulations: 1) Commercial media such as FluorSave (Calbiochem) or whatever the name of Molecular Probes' similar product is (Slow Fade, I think). (I have no connection to either of these companies.) 2) A product from Mallinckrodt diagnostics division called Conray (sold as a contrast agent for X-rays, I think). 3) Homemade formulas containing n-propyl-gallate in buffered glycerol (I can send references/recipes to anyone interested). Have you tried any of these? If so, I'm stumped! Jim -- Jim Hutchins [] E-Mail: jbh@anat.umsmed.edu Asst Prof of Anatomy [] Asst Prof of Neurology Univ Mississippi Med Ctr [] Jackson, MS From owner-immunology@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!news From: sfm@manduca.neurobio.arizona.edu (Stephen Matheson) Newsgroups: bionet.immunology Subject: Re: Fading problem with flourecein labelling Message-ID: <2djdoe$svo@organpipe.uug.arizona.edu> Date: 2 Dec 93 00:43:58 GMT References: <2div26$6hv@netnews.upenn.edu> Organization: University of Arizona UNIX Users Group Lines: 31 NNTP-Posting-Host: manduca.neurobio.arizona.edu From article <2div26$6hv@netnews.upenn.edu>, by cychan@mail.sas.upenn.edu (Cecilia Y Chan): > I am having a lot of problems with fading using flourescein label for Ab. > I have tried various mounting reagents, but they all seem to fade over a > period of several weeks. With many of the mounting reagents, there also > seems to be high background and the dye seems to disperse out of the > cells. But if I don't mount them, the dye fades when it's exposed to light. I'm currently using a polyvinyl alcohol-based formulation that contains DABCO as an anti-fading agent. I got the recipe from _Culturing Nerve Cells_ by Banker and Goslin, MIT Press, 1991. It's the best I've ever tried. DABCO, btw, is 1,4-diazoabicyclo[2,2,2]octane. A few comments, though. First, you probably know that fluorescence of fluorescein is maximal above pH about 8.5. Second, the best I've ever heard in terms of longevity of fluorescence is Banker & Goslin's claim of "months" with the polyvinyl alcohol-based medium. So your "several weeks" is not all that bad. Third, there are some new variants of fluorescein that exhibit lower background and less fading. We've been pleased with the results using dichlorotriazinylamino fluorescein (DTAF) from Jackson. Last, if there's any way to switch to a *real* fluorophore like Cy3 or lissamine rhodamine, do it. > I would really appreciate any suggestions or references for articles > regarding this topic. The Jackson catalog is an excellent source of information. -- Steve Matheson Program in Neuroscience University of Arizona sfm@neurobio.arizona.edu From owner-immunology@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!bcm!biosci!WRAIR-EMH1.ARMY.MIL!dexper From: dexper@WRAIR-EMH1.ARMY.MIL Newsgroups: bionet.immunology Subject: Immunol. Contraception: Societal Issues Message-ID: <9311250412.AA03681@net.bio.net> Date: 25 Nov 93 02:14:00 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 16 I think that there are two issues: (1) Should a contraceptive vaccine be made at all? and (2) Are the vaccines currently in clinical trials safe? You might begin by asking the people who are protesting the clinical trials whether they would be in favor of a vaccine if they could be satisfied that it is safe. Some feminists have said that birth control is a technique for "blaming" women for population problems. If safety is the only issue here, you might point out that vaccines do not go into clinical trials until they have shown safety and efficacy in animal tests. And of course safety testing is the first phase of clinical trials. You might also point out that decisions to develop vaccines are usually not made by scientists alone but by public agencies spending tax money and which are accountable to the taxpayer. If vaccines are developed by private firms, the licensing process is still by a public agency. Jack Komisar dexper@wrair-emh1.army.mil From owner-immunology@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!HAL.HAHNEMANN.EDU!dalyt From: dalyt@HAL.HAHNEMANN.EDU Newsgroups: bionet.immunology Subject: December 2, 1993 Message-ID: <00976694.6C4408A0.16524@hal.hahnemann.edu> Date: 2 Dec 93 17:08:34 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 24 Hello Folks. I,m looking for a source of the Fc fragment from MOUSE IgG. Cappel sells the Fc fragment from rabbit, goat and human IgG but not mouse. I've considered preparing some myself but there is a time factor and the cost of protein-A/protein-G, etc.. Any information would be greatly appreciated. Since I do not read this BB all that often, please send any responses via e-mail to dalyt@hal.hahnemann.edu. Thank you in advance. T. ---------------------------------------------------------------------------- THOMAS M. DALY / / / / DEPT. OF MICRO. & IMMUNO. MS 405 Voice (215) 762-1903 /___/ / / HAHNEMANN UNIVERSITY Fax (215) 762-8075 / / / / BROAD & VINE STS., DALYT@hal.hahnemann.edu / / /___/ PHILA., PA 19102 ---------------------------------------------------------------------------- From owner-immunology@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!ETSUACAD.ETSU.EDU!JB5369 From: JB5369@ETSUACAD.ETSU.EDU (GORDON BETTS) Newsgroups: bionet.immunology Subject: IFN TAU Message-ID: <9312021625.AA08526@net.bio.net> Date: 2 Dec 93 16:25:49 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 23 RECENTLY JACK KOMISAR WROTE: There is no interferon tau. There was a letter to the editor earlier this year, probably in Nature, dealing with the problem. Apparently some people use a tau on their word processors to stand for a gamma, since there is no ASCII code for a lower-case gamma. They intend to remove the tau before submitting the paper and draw in a gamma by hand. However, sometimes they forget, and several papers have appeared about interferon tau. Jack Komisar dexper@wrair-emh1.army.mil ALTHOUGH SOMEONE MAY HAVE CONFUSED THEIR GREEK ALPHABET, THERE IS AN IFN TAU WITH 80+% SEQUENCE HOMOLOGY WITH IFN'S ALPHA AND BETA. IT HAS NO MORE HOMOLOGY WITH IFN GAMMA THAN DO THE ALPHA AND BETA. Distrust Simplicity. Simplify, simplify. Alfred North Whitehead Henry David Thoreau ================================================================== J. Gordon Betts East Texas State University Dept. of Biological Sciences Commerce, TX 75429-3011 JB5369@ETSUACAD.ETSU.EDU From owner-immunology@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!ETSUACAD.ETSU.EDU!JB5369 From: JB5369@ETSUACAD.ETSU.EDU (GORDON BETTS) Newsgroups: bionet.immunology Subject: INTERFERON TAU Message-ID: <9312021618.AA08229@net.bio.net> Date: 2 Dec 93 16:13:38 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 26 IN A RECENT NOTE, HORST IBELGAUFTS WRITES: Dear netters, what is interferon tau? can anyone also provide me with a suitable reference? thanks a lot. IN REPLY: INTERFERON TAU IS STRUCTURALLY SIMILAR TO IFN'S ALPHA AND BETA WITH SIMILAR FUNCTIONS. IT IS PRODUCED BY THE EMBRYONIC TROPHOBLAST OF COWS, SHEEP AN GOATS AT ABOUT DAY 11 OF PREGNANCY (DEPENDING ON THE EXACT SPECIES). IT INHIBITS PROSTAGLANDIN F2 ALPHA PRODUCTION WHICH WOULD OTHERWISE CAUSE REGRESSION OF THE CORPUS LUTEUM AND THE CONSEQUENT NEXT ESTROUS CYCLE WOULD ENSUE. THUS IT IS THE PROTEIN RESPONSIBLE FOR MATERNAL RECOGNITION OF PREGNANCY IN THESE SPECIES. IT WAS ORIGINALLY NAME BOVINE (OR OVINE OR CAPRINE) TROPHOBLAST PROTEIN-1 TO SIGNIFY IT AS THE FIRST MAJOR PROTEIN PRODUCED BY THE TROPHOBLAST. GOOD REFERE NCES TO SEARCH WOULD BE PAPERS BY P.J. HANSEN, R.M. ROBERTS OR F.W. BAZER. BAZER AND ROBERTS WERE RESPONSIBLE FOR THE EARLY DISCOVERY OF THIS IFN. Distrust Simplicity. Simplify, simplify. Alfred North Whitehead Henry David Thoreau ================================================================== J. Gordon Betts East Texas State University Dept. of Biological Sciences Commerce, TX 75429-3011 JB5369@ETSUACAD.ETSU.EDU From owner-immunology@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!wupost!medicine.wustl.edu!wums.wustl.edu!wetsel_r From: wetsel_r@wums.wustl.edu Newsgroups: bionet.immunology Subject: RE: CD62 (PADGEM) Ab for flow cytometry Message-ID: <2DEC93.12360729@wums.wustl.edu> Date: 2 Dec 93 17:36:07 GMT References: Organization: Washington University in St. Louis, Medical Library Lines: 29 NNTP-Posting-Host: msnews.wustl.edu In a previous article, mpkedra@cyf-kr.edu.pl (Dariusz Kedra ) wrote: >Dear Networkers, >I'm looking for any comercial or noncomercial source of >Ab against CD62 (PADGEM). >Thanks in advance. >Darek Kedra >Dept of Pathophysiology >University School of Medicin, >Cracow, Poland >tel: (48) (12) 188-484 >fax: 210-993 >e-mail:mpkedra@lfs.cyf-kr.edu.pl Darek: Try Harlan Bioproducts, P.O.Box 29176, Indianapolis, IN 46229 1-800-9-Science, FAX 317-894-1840. Get one of thier catalogs as they have numerous antibodies to various CD antigens. David =========================================================================== + David L. Haviland, Ph.D. Internet:"haviland@kids.wustl.edu" + + Washington Univ. School of Med. A.K.A : The Compiler + + Dept. of Peds./Pulm. Box 8116 ICBM-Net : Just hit St. Louis + + 400 S. Kingshighway &-6 <- User is Brain Dead... + + St. Louis, MO 63110 FAX: 314-454-2476 + + (314) 454-6076 + =========================================================================== From owner-immunology@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!spool.mu.edu!howland.reston.ans.net!usenet.ins.cwru.edu!news.ecn.bgu.edu!willis1.cis.uab.edu!right.dom.uab.edu!user From: holland@gasmac.dom.uab.edu (Steve Holland) Newsgroups: sci.med,bionet.general,bionet.immunology Subject: Anyone at SIU/Springfield? Message-ID: Date: 2 Dec 93 18:00:04 GMT Sender: root@cis.uab.edu (Operator) Followup-To: sci.med Organization: UAB Lines: 10 Xref: biosci sci.med:26620 bionet.general:6709 bionet.immunology:766 I would like to correspond with someone at SIU/Springfield to get some information about what it is like there. I am interested in a position at the medical school in the Gastroenterology division. I have visited there, and am interested in other insights from people familiar with the place. If willing, please e-mail me at holland@gasmac.dom.uab.edu Thanks, Steve Holland From owner-immunology@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!cs.utexas.edu!swrinde!dptspd!TAMUTS.TAMU.EDU!not-for-mail From: sac5001@TAMUTS.TAMU.EDU (Scott Alexander Coonrod) Newsgroups: bionet.immunology Subject: Re: Fading problem with flourecein labelling Message-ID: <2dl67s$4hd@TAMUTS.TAMU.EDU> Date: 2 Dec 93 16:47:56 GMT References: <2div26$6hv@netnews.upenn.edu> Organization: Texas A&M University, College Station Lines: 4 NNTP-Posting-Host: tamuts.tamu.edu Have you tried Slow Fade mounting media from Molecular Probes It has worked well for me in the past. Also make sure to keep the slides in the dark and in the fridge. Regards sac5001@tamu.edu From owner-immunology@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!europa.eng.gtefsd.com!howland.reston.ans.net!vixen.cso.uiuc.edu!uchinews!mica!TEllis From: tellis@mica.meddean.luc.edu (TEllis) Newsgroups: bionet.immunology Subject: Re: Source of IL12? Message-ID: <2dldft$1of@mica.meddean.luc.edu> Date: 2 Dec 93 18:51:27 GMT References: <9311300331.AA15887@worldlink.worldlink.com> Sender: -Not-Authenticated-[3881] Organization: Loyola University of Chicago Lines: 7 NNTP-Posting-Host: slip1.meddean.luc.edu X-Posted-From: InterNews 1.0@mica.meddean.luc.edu. Xdisclaimer: No attempt was made to authenticate the sender's name. Recombinant human IL-12 is available from R&D Systems, Minneapolis (Catalog number 219-IL). R&D can be reached at (800) 343-7475. We have used this preparation and found it to be quite satisfactory for our purposes. Good Luck. From owner-immunology@net.bio.net Thu Dec 02 22:00:00 1993 Path: biosci!lhc!darwin.sura.net!news-feed-2.peachnet.edu!emory!swrinde!cs.utexas.edu!uunet!munnari.oz.au!ariel.ucs.unimelb.EDU.AU!ucsvc.ucs.unimelb.edu.au!lugb!news From: BOTDJL@LURE.LATROBE.EDU.AU (LOWEN,Darren) Newsgroups: bionet.immunology Subject: separation of light and heavy chains Message-ID: <1993Dec2.015636.6733@lugb.latrobe.edu.au> Date: 2 Dec 93 01:56:36 GMT Sender: news@lugb.latrobe.edu.au (USENET News System) Distribution: bionet Organization: La Trobe University Lines: 10 X-News-Reader: VMS NEWS v1.25 Dear Netters, I would be very grateful if someone could point me in the right direction for a protocol which is designed for the separation and purification of light chains from an IgG monoclonal Ab without contaminating heavy chains. Thanks in advance for any help that you may be able to offer. Darren Lowen botdjl@lure.latrobe.edu.au From owner-immunology@net.bio.net Thu Dec 02 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!cs.utexas.edu!swrinde!menudo.uh.edu!nuchat!texhrc!mjw@texaco.com From: wolskmj@Texaco.com (Mike Wolski) Newsgroups: bionet.immunology Subject: Seeking any information regarding Chronic ITP Keywords: ITP, Thrombocytopenia Message-ID: <1993Dec3.003454.19598@texhrc.uucp> Date: 3 Dec 93 00:34:54 GMT Sender: wolskimj@Texaco.com (Mike Wolski 6690) Organization: Texaco Lines: 18 Nntp-Posting-Host: mjwolski Does anyone have any information regarding an auto-immune disorder know as ITP? My 4-year old son has been diagnosed as having Chronic ITP 10 months ago, and I'd like to hear from others familiar with it and its treatment. Thanks! -- Regards, Mike Wolski Sys/Net/Zone Admin. gearhead@texaco.com "Race forever; Work whenever...." - - Follow Your Dreams - - From owner-immunology@net.bio.net Fri Dec 03 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!uknet!pipex!howland.reston.ans.net!europa.eng.gtefsd.com!darwin.sura.net!hearst.acc.Virginia.EDU!maxwell!mgk2r From: mgk2r@Virginia.EDU (Michael G. Kurilla) Newsgroups: bionet.immunology Subject: Re: Immunol. Contraception Message-ID: <1993Dec3.162408.20962@Virginia.EDU> Date: 3 Dec 93 16:24:08 GMT References: <931129104509.33182@UABCVSR.cvsr.uab.edu> Distribution: bionet Organization: University of Virginia Lines: 16 txpljfg@UABCVSR.CVSR.UAB.EDU writes: > > The issue is: Are we really talking about contraception here? Many > vaccines tend to induce immunity that is relatively permanent. If this > were the case with an immunologic vaccine, then the result would > effectively be sterilization. > Actually, most vaccines are not permanent, hence the need for booster shots. Data is just now being accumulated on the timespan of effective protection of the recombinant Hep B vaccine. The smallpox vaccine (still used by those of use who work with vaccinia virus) has only recently been upgraded from 3 year booster to 10 year booster. Mike K From owner-immunology@net.bio.net Fri Dec 03 22:00:00 1993 Path: biosci!daresbury!bioftp.unibas.ch!rc1!ub4b!EU.net!howland.reston.ans.net!usenet.ins.cwru.edu!news.ecn.bgu.edu!willis1.cis.uab.edu!beagley.dom.uab.edu!user From: holland@gasmac.dom.uab.edu (Steve Holland) Newsgroups: bionet.immunology Subject: Re: Seeking any information regarding Chronic ITP Message-ID: Date: 3 Dec 93 16:01:14 GMT References: <1993Dec3.003454.19598@texhrc.uucp> Sender: root@cis.uab.edu (Operator) Followup-To: bionet.immunology Organization: UAB div GI Lines: 13 In article <1993Dec3.003454.19598@texhrc.uucp>, wolskmj@Texaco.com (Mike Wolski) wrote: > > > Does anyone have any information regarding an auto-immune disorder > know as ITP? My 4-year old son has been diagnosed as having Chronic > ITP 10 months ago, and I'd like to hear from others familiar with it and > its treatment. Thanks! Put this question to sci.med. There are a lot of excellent generalists there that might be willing to discuss the case. Steve Holland From owner-immunology@net.bio.net Sun Dec 05 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!uknet!pipex!howland.reston.ans.net!xlink.net!scsing.switch.ch!swidir.switch.ch!univ-lyon1.fr!jussieu.fr!zaphod.crihan.fr!news.univ-rennes1.fr!pan.univ-rennes1.fr!user From: Choukrou@univ-rennes1.fr (BOUHALLIER H.) Newsgroups: bionet.immunology Subject: Hypereosinophilia=leucemia? Message-ID: Date: 6 Dec 93 13:58:40 GMT Followup-To: bionet.immunology Organization: Geosciences Lines: 6 NNTP-Posting-Host: pan.univ-rennes1.fr Hello net users, Is it possible to treat a strong hypereosinophilia (eosinophils>40 000) knowing that no parasitic organism can be identified so far? Thank you for replies! From owner-immunology@net.bio.net Sun Dec 05 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!europa.eng.gtefsd.com!howland.reston.ans.net!xlink.net!scsing.switch.ch!swidir.switch.ch!univ-lyon1.fr!jussieu.fr!zaphod.crihan.fr!news.univ-rennes1.fr!pan.univ-rennes1.fr!user From: Choukrou@univ-rennes1.fr (BOUHALLIER H.) Newsgroups: bionet.immunology Subject: Hypereosinophilia=leucemia? Message-ID: Date: 6 Dec 93 13:58:40 GMT Followup-To: bionet.immunology Organization: Geosciences Lines: 6 NNTP-Posting-Host: pan.univ-rennes1.fr Hello net users, Is it possible to treat a strong hypereosinophilia (eosinophils>40 000) knowing that no parasitic organism can be identified so far? Thank you for replies! From owner-immunology@net.bio.net Sun Dec 05 22:00:00 1993 Path: biosci!lhc!darwin.sura.net!howland.reston.ans.net!xlink.net!scsing.switch.ch!swidir.switch.ch!univ-lyon1.fr!jussieu.fr!zaphod.crihan.fr!news.univ-rennes1.fr!pan.univ-rennes1.fr!user From: Choukrou@univ-rennes1.fr (BOUHALLIER H.) Newsgroups: bionet.immunology Subject: Hypereosinophilia=leucemia? Message-ID: Date: 6 Dec 93 13:58:40 GMT Followup-To: bionet.immunology Organization: Geosciences Lines: 6 NNTP-Posting-Host: pan.univ-rennes1.fr Hello net users, Is it possible to treat a strong hypereosinophilia (eosinophils>40 000) knowing that no parasitic organism can be identified so far? Thank you for replies! From owner-immunology@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!pipex!zaphod.crihan.fr!news.univ-rennes1.fr!roland.univ-rennes1.fr!nmenoux From: nmenoux@roland.univ-rennes1.fr ( Nicolas Menoux) Newsgroups: bionet.immunology Subject: Human Class1-2 Histocompatibility Antigen Message-ID: <2e1ns5$pd@news.univ-rennes1.fr> Date: 7 Dec 93 11:02:29 GMT Organization: Universite de Rennes 1, France Lines: 20 NNTP-Posting-Host: roland.univ-rennes1.fr X-Newsreader: TIN [version 1.2 PL0] Hello, I am a novice in immunology but I am really intersted in. I have recently read an article about the human Histocompatibility Antigen which present at the cell surface peptides. (I am sorry for vocabulary, I do not know the exact translation, the article was in french.) The role played by this Human Histo.. to vehicule peptides, is it the only role ? Can you help me in my long research !! I have no background in medicine, pharmacology and immunology but it is a passion. I am really intersted in research. I hope, one day, use my Computer Science and Electyrical Engineering diploma in such research domain. Thank you a lot! Nicolas MENOUX -------- Nicolas.Menoux@univ-rennes1.fr From owner-immunology@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!europa.eng.gtefsd.com!emory!cs.utk.edu!willis1.cis.uab.edu!doma1.immun.uab.edu!user Newsgroups: bionet.immunology Subject: Re: Hypereosinophilia=leucemia? Message-ID: Date: 6 Dec 93 22:44:36 GMT References: Sender: root@cis.uab.edu (Operator) Followup-To: bionet.immunology Organization: Univ. of Alabama at Birmingham Lines: 18 In article , Choukrou@univ-rennes1.fr (BOUHALLIER H.) wrote: > > Hello net users, > > Is it possible to treat a strong hypereosinophilia (eosinophils>40 000) > knowing that no parasitic organism can be identified so far? > Try assaying the serum for interleukin-5. I have seen a hypereosiniphila patient have correlative high IL-5 levels. If IL-5 is up, possible ways to treat it might be with IFN gamma, sIL-5R, immunosuppression especially anything targeted to T cells, maybe even passive transfer of anti-IL-5. WARNING: I am not a physician, but a humble grad student so these are merely speculations. I'd be curious to see what the status quo on treatment is. Allen Black Dept. of Microbiology U. of Alabama at Birmingham From owner-immunology@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!pipex!howland.reston.ans.net!spool.mu.edu!umn.edu!lynx.unm.edu!dns1.NMSU.Edu!smori From: smori@nmsu.edu (Shahram Mori) Newsgroups: bionet.immunology Subject: IL-2 interesting finding Message-ID: <2e066bINNsoh@dns1.NMSU.Edu> Date: 6 Dec 93 20:54:35 GMT Organization: New Mexico State University, Las Cruces, NM Lines: 14 NNTP-Posting-Host: dante.nmsu.edu X-Newsreader: TIN [version 1.2 PL1] Dear netters, I read in a recent Science article, that IL-2 deficient mice had normal CD4 and CD8 responses while their NK cells activity was drastically reduced. I was very surprised. IL-2 is supposed to be an important factor in the differentiation of T-cells. There has been suggestions ( By Jonas Salk and Peter Bretscher) that a switch in Th-1 to Th-2 is the reason for progression of HIV to AIDS. If IL-2 deficient mice can have normal CMI, then what is it that induces the differentiation of T-cells. How does this affect the idea about Th1 switch to Th2. I would like to hear your ideas on this matter. cheers Shahram e-mai smori@nmsu.edu From owner-immunology@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!news.cs.umb.edu!hsdndev!rutgers!headwall.Stanford.EDU!agate!mendel.Berkeley.EDU!frauwirt From: frauwirt@mendel.Berkeley.EDU (Ken Frauwirth) Newsgroups: bionet.immunology Subject: Re: IL-2 interesting finding Message-ID: <2e0ku5$sa5@agate.berkeley.edu> Date: 7 Dec 93 01:06:13 GMT References: <2e066bINNsoh@dns1.NMSU.Edu> Organization: University of California, Berkeley Lines: 42 NNTP-Posting-Host: mendel.berkeley.edu In article <2e066bINNsoh@dns1.NMSU.Edu>, smori@nmsu.edu (Shahram Mori) writes: |> |> Dear netters, |> I read in a recent Science article, that IL-2 deficient mice had normal |> CD4 and CD8 responses while their NK cells activity was drastically reduced. |> I was very surprised. IL-2 is supposed to be an important factor in the |> differentiation of T-cells. There has been suggestions ( By Jonas Salk and |> Peter Bretscher) that a switch in Th-1 to Th-2 is the reason for |> progression of HIV to AIDS. If IL-2 deficient mice can have normal CMI, then |> what is it that induces the differentiation of T-cells. How does this |> affect the idea about Th1 switch to Th2. |> I would like to hear your ideas on this matter. |> cheers |> Shahram |> e-mai smori@nmsu.edu What seems to be very likely (especially based on similar results of other cytokine knock-outs) is that many of the cytokines have overlapping, if not completely redundant, functions. Also, according to a recent seminar here by Anne O'Gara of DNAX: 1) IL-12 + gamma-IFN will induce CD4+ cells to differentiate into a Th-1 phenotype in vitro, when also supplied with antigen and APC (D.C.'s, which do not induce differentiation without added cytokines). 2) Anti-IL-4 will *also* induce differentiation to Th-1 phenotype, but apparently by a different mechanism (IL-12/IFN + anti-IL-4 gives an additive effect). Since there may be more than one mechanism of inducing Th-1 phenotype, the loss of one mechanism may not have much visible effect on the mice under the conditions tested. However, it is also important to remember that all of these knockout mice live in antiseptic environments and are tested under very defined conditions. It is not unlikely that defects would become evident under the stresses of normal (i.e. non-laboratory) existence. -- Ken Frauwirth BBB IIIII OO K K EEEEE N N frauwirt@mendel.berkeley.edu B B I O O K K E NN N Dept. of Molec. & Cell Bio. BBB I O O KK EEEE N N N Immunology Division B B I O O K K E N NN Univ. of Cal., Berkeley BBB IIIII OO K K EEEEE N N From owner-immunology@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!licr.dn.mu.oz.au!RUNTING From: RUNTING@licr.dn.mu.oz.au Newsgroups: bionet.immunology Subject: purfication of rat antibodies Message-ID: <01H65ZT9UZIA001Z5C@muwayb.ucs.unimelb.edu.au> Date: 6 Dec 93 06:42:30 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 12 Hi netters, being a molecular biologist introduced to the world of monoclonal antibodies by necessity, I'm feeling lost as to how to purify my rat anti mouse monoclonals I have just finished producing. As far as I can see Rat antibodies are notoriously hard to purify. Has anyone out there any advice as to how I should go about purification ? Any help would be much appreciated. Andrew Runting Ph.D. Student, Ludwig Institute for Cancer Research Melbourne, Australia From owner-immunology@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!JUDY.ENG.UCI.EDU!liang From: liang@JUDY.ENG.UCI.EDU (liang) Newsgroups: bionet.immunology Subject: CHINESES_BIOTECH_NET_FOUNDED Message-ID: <9312060501.AA19405@judy.eng.uci.edu> Date: 6 Dec 93 05:01:00 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 12 CBNet (Chinese Biotechnology Network) is a non-profit organization composed of professionals in biological, chemical, medical sciences, engineering and related fields. The CBNet sponsors the Chinese Biotechnology Internet Forum (CBIF) newsletter. To subscribe CBIF, please send an email to Listserv@UCSD.Edu with the message body: Add CB-Net. From owner-immunology@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!JUDY.ENG.UCI.EDU!liang From: liang@JUDY.ENG.UCI.EDU (liang) Newsgroups: bionet.immunology Subject: CHINESES_BIOTECH_NET_FOUNDED Message-ID: <9312061945.AA20510@judy.eng.uci.edu> Date: 6 Dec 93 19:45:58 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 12 CBNet (Chinese Biotechnology Network) is a non-profit organization composed of professionals in biological, chemical, medical sciences, engineering and related fields. The CBNet sponsors the Chinese Biotechnology Internet Forum (CBIF) newsletter. To subscribe CBIF, please send an email to Listserv@UCSD.Edu with the message body: Add CB-Net. From owner-immunology@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!agate!mendel.Berkeley.EDU!frauwirt From: frauwirt@mendel.Berkeley.EDU (Ken Frauwirth) Newsgroups: bionet.immunology Subject: Re: IL-2 Message-ID: <2e31i3$83s@agate.berkeley.edu> Date: 7 Dec 93 22:53:55 GMT References: <199312071711.AA26609@wugate.wustl.edu> Distribution: bionet Organization: University of California, Berkeley Lines: 53 NNTP-Posting-Host: mendel.berkeley.edu In article <199312071711.AA26609@wugate.wustl.edu>, brett@BORCIM.WUSTL.EDU writes: |> Yes, the recent findings on IL-2 have given us much to think about. Ken |> Frauwith and anyone else - you offer the much overused redundancy argument: |> that is, if IL-2 knockouts don't behave as we would predict, then some other |> mechanism must be compensating. While this argument may be insightful, it |> could just be handwaving unless you propose some other mechanism. It was |> interesting to note in the literature earlier this year (Cell, V73, pp147-57) |> that human XSCID diease maps to the IL-2R gamma gene, and mutations at this |> locus were found in these patients. Thus, humans with defective IL-2 signaling |> can be immunodeficient. Although the murine knockouts were of different genes, |> either IL-2 is functioning in ways other than we have previously thought, or |> human and murine IL-2 are not entirely functionally homologous. ANY COMMENTS? |> |> brett@borcim.wustl.edu Whether or not the "redundancy" argument is overused, there are only three explanations for a lack of any visible phenotype in a knockout experiment: 1) The gene is not completely disrupted, and still has some function. 2) The gene is not necessary under the tested conditions, but might be critical under other specific conditions (e.g. to combat a specific class of parasites or viruses). 3) Some other gene product can compensate for the loss, at least enough to give apparently normal phenotype. Under more stressful conditions, this compensation may not be enough for normal function, similar to explanation 2). (A fourth explanation is that the gene is truly dispensable, with no real function, but let's discount that.) One way to explain an apparent redundancy, especially in light of the putative IL-2R/XSCID connection, is the possibility of multiple ligands for the IL-2 receptor. I do not know if a search for other IL-2-like genes has been undertaken, but it is not unreasonable to think that there may be related genes. However, it is not necessary that the the two ligands be very closely related in sequence. Many receptors are capable of binding multiple ligands, even unrelated molecules (this is especially true of neurotransmitter receptors). In fact, the EGF receptor has two known ligands, EGF and TGF-alpha, which are the products of distinct genes. I do not know the degree of homology, if any, between the two growth factors, but the existence of multiple ligands for grwoth factor receptors (including IL-2) could help explain the lack of a dramatic phenotype for growth factor gene knockouts. One way of addressing this is to disrupt the gene(s) for the receptor, rather than the ligand. -- Ken Frauwirth BBB IIIII OO K K EEEEE N N frauwirt@mendel.berkeley.edu B B I O O K K E NN N Dept. of Molec. & Cell Bio. BBB I O O KK EEEE N N N Immunology Division B B I O O K K E N NN Univ. of Cal., Berkeley BBB IIIII OO K K EEEEE N N "When a rocket loves a space station *very very* much..." - T. Servo From owner-immunology@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!cs.utexas.edu!swrinde!emory!news-feed-2.peachnet.edu!ukma!seqanal.mi.uky.edu!woodward From: woodward@seqanal.mi.uky.edu (Jerry Woodward) Newsgroups: bionet.immunology Subject: POSTDOCTORAL POSITION AVAILABLE-IMMUNOLOGY Message-ID: <2e2opj$mel@s.ms.uky.edu> Date: 7 Dec 93 20:24:19 GMT Organization: University of Kentucky, Dept. of Math Sciences Lines: 21 NNTP-Posting-Host: seqanal.mi.uky.edu X-Newsreader: TIN [version 1.1 PL9] A postdoctoral position is available immediately to study mechanisms of immunological tolerance and autoimmunity in a transgenic mouse model. This model involves the production of transgenic mice expressing various antigens or immunoregulatory molecules in different parts of the eye. These mice can then be used to evaluate mechanisms of tolerance within immunologically privileged sites. Experience in cellular immunology, mouse handling, and some molecular biology is desirable. Applicants must be U.S. citizens or permanent residents. Please send a CV, a description of your research, and three letters of reference to: Jerold G. Woodward, Ph.D. University of Kentucky Medical Center Lexington, KY 40536-0084 Phone: (606) 233-5538 Fax: (606) 257-8994 E-mail: woodward@seqanal.mi.uky.edu From owner-immunology@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!pipex!howland.reston.ans.net!cs.utexas.edu!not-for-mail From: MEGPRASA@thcme.indstate.edu ("PRASAD S. KULKARNI" ) Newsgroups: bionet.general,bionet.immunology,bionet.molbio.gene-linkage,bionet.molbio.hiv,bionet.molbio.methds-reagnts,bionet.virology Subject: Request for sample of RSV-LTR for use as a promoter Message-ID: <68346D5CE9@thcme.indstate.edu> Date: 7 Dec 93 19:41:38 GMT Sender: daemon@cs.utexas.edu Organization: UTexas Mail-to-News Gateway Lines: 19 Xref: biosci bionet.general:6774 bionet.immunology:784 bionet.molbio.gene-linkage:269 bionet.molbio.hiv:271 bionet.molbio.methds-reagnts:9708 bionet.virology:326 NNTP-Posting-Host: cs.utexas.edu Hi, I would like to request readers of this newsgroup to send me a sample of the RSV-LTR for use as a promoter for a gene that we need to express in human hematopoietic cell lines. I would also like to receive comments/suggestions about the suitability of the RSV-LTR as a promoter in this situation (i.e expression of a gene in a human hematopoietic cell line). My mailing address is: Prasad Kulkarni Department of Life Sciences Indiana State University Terre Haute, IN 47809 U.S.A Fax: (812) 237-4480 e.mail: MEGPRASA@thcme.indstate.edu Thank you very much. Prasad From owner-immunology@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!BORCIM.WUSTL.EDU!brett From: brett@BORCIM.WUSTL.EDU Newsgroups: bionet.immunology Subject: IL-2 Message-ID: <199312071711.AA26609@wugate.wustl.edu> Date: 7 Dec 93 17:11:18 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 13 Yes, the recent findings on IL-2 have given us much to think about. Ken Frauwith and anyone else - you offer the much overused redundancy argument: that is, if IL-2 knockouts don't behave as we would predict, then some other mechanism must be compensating. While this argument may be insightful, it could just be handwaving unless you propose some other mechanism. It was interesting to note in the literature earlier this year (Cell, V73, pp147-57) that human XSCID diease maps to the IL-2R gamma gene, and mutations at this locus were found in these patients. Thus, humans with defective IL-2 signaling can be immunodeficient. Although the murine knockouts were of different genes, either IL-2 is functioning in ways other than we have previously thought, or human and murine IL-2 are not entirely functionally homologous. ANY COMMENTS? brett@borcim.wustl.edu From owner-immunology@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!agate!mendel.Berkeley.EDU!frauwirt From: frauwirt@mendel.Berkeley.EDU (Ken Frauwirth) Newsgroups: bionet.immunology Subject: Re: Human Class1-2 Histocompatibility Antigen Message-ID: <2e32kt$83s@agate.berkeley.edu> Date: 7 Dec 93 23:12:29 GMT References: <2e1ns5$pd@news.univ-rennes1.fr> Organization: University of California, Berkeley Lines: 41 NNTP-Posting-Host: mendel.berkeley.edu In article <2e1ns5$pd@news.univ-rennes1.fr>, nmenoux@roland.univ-rennes1.fr ( Nicolas Menoux) writes: |> Hello, |> |> I am a novice in immunology but I am really intersted in. |> I have recently read an article about the human Histocompatibility Antigen |> which present at the cell surface peptides. (I am sorry for vocabulary, |> I do not know the exact translation, the article was in french.) |> |> The role played by this Human Histo.. to vehicule peptides, is it the only |> role ? |> |> Can you help me in my long research !! I have no background in medicine, |> pharmacology and immunology but it is a passion. I am really intersted in |> research. I hope, one day, use my Computer Science and Electyrical Engineering |> diploma in such research domain. |> |> Thank you a lot! |> |> Nicolas MENOUX |> -------- |> Nicolas.Menoux@univ-rennes1.fr |> It is my understanding (as a graduate student in a lab which studies antigen processing and presentation) that the only known function of the MHC proteins is to present peptide antigens for recognition by T cell receptors. Class I MHC is responsible for presenting peptides derived from proteins produced inside the cell (including viral proteins), while Class II MHC appears to be responsible for presenting peptides derived from cell-external (and perhaps cell-surface as well) proteins, including those phagocytosed by macrophages. If you have more specific questions, feel free to e-mail me at the address below. -- Ken Frauwirth BBB IIIII OO K K EEEEE N N frauwirt@mendel.berkeley.edu B B I O O K K E NN N Dept. of Molec. & Cell Bio. BBB I O O KK EEEE N N N Immunology Division B B I O O K K E N NN Univ. of Cal., Berkeley BBB IIIII OO K K EEEEE N N "When a rocket loves a space station *very very* much..." - T. Servo From owner-immunology@net.bio.net Tue Dec 07 22:00:00 1993 Path: biosci!ccs.carleton.ca!hmehrani From: hmehrani@ccs.carleton.ca (HOSSEIN MEHRANI) Newsgroups: bionet.immunology Subject: Doctoral position in Brazil, USP Message-ID: <9312080558.AA26340@alfred.ccs.carleton.ca> Date: 8 Dec 93 05:58:24 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 27 ATENTION (Brazilians only): ONE DOUTORAL POSITION AVAILABLE AT UNIVEERSITY OF SAO PAULO TO WORK WITH CHEMICAL MODELS OF INFLAMATION, THE ROLE OF OXYRADICALS AND IRON IONS. get in contact with Dr.Marcelo Hermes-Lima Departamento de Imunologia, ICB, USP Internet: hmehrani@ccs.carleton.ca FAX: 613-788-4389 I'm starting a new lab in march/abril and I'm looking for entusiastic canditates. We will apply for a CNPq or FAPESP fellowship. Sincerely: Dr.Marcelo H.-Lima xxsxxhxxxosxxxxxxxxxxxxxxxxxxxxxxxxxhxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxoxxu Other positions also available: Mestrado (1), Aperfeicoamento (3)3 , Postdoctoral fellow (1). "Iniciacao" (3) XCCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX From owner-immunology@net.bio.net Tue Dec 07 22:00:00 1993 Path: biosci!TUI.LINCOLN.AC.NZ!MCFARLAN From: MCFARLAN@TUI.LINCOLN.AC.NZ Newsgroups: bionet.immunology Subject: software for teaching Message-ID: <12578951AB3@tui.lincoln.ac.nz> Date: 8 Dec 93 00:54:56 GMT Sender: daemon@net.bio.net Distribution: bionet Organization: Lincoln University Lines: 47 Greetings: I have an interest in procuring software for undergraduate and graduate teaching in Immunology and /or Microbiology. Any knowledge on commercially available or homemade kits would be welcome. Similiarly, if anybody has information on Immunology videos available for teaching purposes I would be grateful. Thanks, Robin. Robin McFarlane, Principal Research Officer, Animal and Veterinary Science Group, Lincoln University, NEW ZEALAND. Phone: 64-03-3252811 (ext 8176), Fax: 64-03-3253851 e mail:McFarlane@lincoln.ac.nz From owner-immunology@net.bio.net Tue Dec 07 22:00:00 1993 Path: biosci!POST.ITS.MCW.EDU!vkurup From: vkurup@POST.ITS.MCW.EDU (Viswanath P. Kurup PhD) Newsgroups: bionet.immunology Subject: Post-doctoral position Message-ID: <9312082032.AA10192@post.its.mcw.edu> Date: 8 Dec 93 20:32:50 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 9 A post-doctoral position available immediately for an Immunologist-Molecular Biologist with experience in DNA sequencing and protein expression at the Allergy-Immunology Division of the Medical College of Wisconsin at Milwaukee. Send C.V. and 3 references to: Viswanath P. Kurup, Proferofessor, department of Medicine, Medical college of wisconsin and research service-151I, 5000 west National avenue, Milwaukee, WI. 53295-1000. Fax.(414) 382-5374. From owner-immunology@net.bio.net Wed Dec 08 22:00:00 1993 Path: biosci!daresbury!zeta.bmc.uu.se!corax.udac.uu.se!sunic!EU.net!howland.reston.ans.net!news.moneng.mei.com!uwm.edu!caen!zip.eecs.umich.edu!umn.edu!lynx.unm.edu!dns1.NMSU.Edu!smori From: smori@nmsu.edu (Shahram Mori) Newsgroups: bionet.immunology Subject: IL-2's immune function Message-ID: <2e89obINNcpb@dns1.NMSU.Edu> Date: 9 Dec 93 22:44:27 GMT Organization: New Mexico State University, Las Cruces, NM Lines: 19 NNTP-Posting-Host: dante.nmsu.edu X-Newsreader: TIN [version 1.2 PL1] Dear netters, What surprises me about this study is that In Vitro the effect of IL-2 is very important. The in vitro anti-viral studies showed a lack of T-cell response. This can only mean that there is another important factor that may work synergistically with IL-2. Ken mentioned that it might be a still functional IL-2. According to the science paper (Vol 262 : 1059) These mice were deficient in IL-2 ( IL-2 -/-) therefore there can not be a question about a semi-functional IL-2. The mice were assayed by infecting them with Vaccinia Virus and LCMV.I don't think that the antiseptic environment would affect these particular results. Is there an IL-2 co-factor. Do we have the true IL-2 receptor? Thanks Shahram Mori Program in Molecular Biology Department of Chemistry Box 3C NMSU Las cruces NM 88003 From owner-immunology@net.bio.net Wed Dec 08 22:00:00 1993 Path: biosci!daresbury!zeta.bmc.uu.se!corax.udac.uu.se!sunic!EU.net!howland.reston.ans.net!cs.utexas.edu!uunet!olivea!news.bu.edu!rocket1 From: rocket1@bu.edu (Richard Near) Newsgroups: bionet.immunology Subject: cd44 and LFA1 on stroma Message-ID: <2e7rea$a8d@news.bu.edu> Date: 9 Dec 93 18:40:10 GMT Organization: Boston University Lines: 12 NNTP-Posting-Host: acs3.bu.edu X-Newsreader: TIN [version 1.2 PL0] We have been noting the expression of CD44 (pgp-1) and LFA-1 in unexpected places, on bone marrow stromal cells and on lymph node stromal cells. Has anyone else seen or heard of this? (The stains are relatively strong with commercial antibodies and using several isotype control antibodies). Rick for Dave Sherr email rocket1@acs.bu.edu Thanks From owner-immunology@net.bio.net Thu Dec 09 22:00:00 1993 Path: biosci!bcm!cs.utexas.edu!swrinde!gatech!willis1.cis.uab.edu!doma8.immun.uab.edu!user Newsgroups: bionet.immunology Subject: Re: IL-2's immune function Message-ID: Date: 10 Dec 93 21:24:53 GMT References: <2e89obINNcpb@dns1.NMSU.Edu> Sender: root@cis.uab.edu (Operator) Followup-To: bionet.immunology Organization: Univ. of Alabama at Birmingham Lines: 40 In article <2e89obINNcpb@dns1.NMSU.Edu>, smori@nmsu.edu (Shahram Mori) wrote: > > > Dear netters, > What surprises me about this study is that In Vitro the effect of IL-2 is > very important. The in vitro anti-viral studies showed a lack of T-cell > response. This can only mean that there is another important factor that > may work synergistically with IL-2. Ken mentioned that it might be a still > functional IL-2. According to the science paper (Vol 262 : 1059) These mice > were deficient in IL-2 ( IL-2 -/-) therefore there can not be a question about > a semi-functional IL-2. The mice were assayed by infecting them with Vaccinia > Virus and LCMV.I don't think that the antiseptic environment would affect > these particular results. Is there an IL-2 co-factor. Do we have the true > IL-2 receptor? IMHO, I think it is clear that both IL-4 and IL-7 can in some cases act as an IL-2 indepedant T cell growth factors just like IL-2. IL-4 is not the mode of action in this transgenic model though, but they don't discount other factors. I would not be surprised if there are even more "TCGF"s than these though. If you look at the IL-2 receptor you'll see it has 3 subunits. Just as oncostatin M, LIF, IL-6 and CNTF have different "receptors" but all share gp130 signalling molecule in the receptor complex, it would not be far fetched to think that other molecules may appropriate an IL-2R subunit. Also it seems that the IL-2beta,IL-2gamma, IL-4,IL-7,IL-9, and EPO receptors are in the same family. This too may explain overlapping function. Check out "Cytokine receptors and and signal transduction" in FASEB Journal Dec. 1992 vol.6, p3387 by Taga and Kishimoto, its a pretty good review and tends to highlight the similarities between the diferrent cytokine receptors. Anyway, I guess I'm kinda happy that IL-2 isn't the ultimate cytokine, that would be too simple and kinda boring. Allen Black Dept. of Microbiology Univ. of Birmingham at Alabama "I am not a scientist, but I've seen one on T.V." From owner-immunology@net.bio.net Thu Dec 09 22:00:00 1993 Path: biosci!UABCVSR.CVSR.UAB.EDU!txpljfg From: txpljfg@UABCVSR.CVSR.UAB.EDU Newsgroups: bionet.immunology Subject: Re: IL-2's immune function Message-ID: <931210170214.22578@UABCVSR.cvsr.uab.edu> Date: 10 Dec 93 23:02:14 GMT References: <9312102152.AA29430@net.bio.net> Sender: daemon@net.bio.net Distribution: bionet Lines: 48 > In article <2e89obINNcpb@dns1.NMSU.Edu>, smori@nmsu.edu (Shahram Mori) > wrote: ><> > > > IMHO, I think it is clear that both IL-4 and IL-7 can in some cases act > as an IL-2 indepedant T cell growth factors just like IL-2. IL-4 is not > the mode of action in this transgenic model though, but they don't discount > other factors. I would not be surprised if there are even more "TCGF"s than > these though. If you look at the IL-2 receptor you'll see it has 3 > subunits. Just as oncostatin M, LIF, IL-6 and CNTF have different > "receptors" but all share gp130 signalling molecule in the receptor > complex, it would not be far fetched to think that other molecules may > appropriate an IL-2R subunit. Also it seems that the IL-2beta,IL-2gamma, > IL-4,IL-7,IL-9, and EPO receptors are in the same family. This too may > explain overlapping function. Check out "Cytokine receptors and and signal > transduction" in FASEB Journal Dec. 1992 vol.6, p3387 by Taga and > Kishimoto, its a pretty good review and tends to highlight the similarities > between the diferrent cytokine receptors. Anyway, I guess I'm kinda happy > that IL-2 isn't the ultimate cytokine, that would be too simple and kinda > boring. > > > > Allen Black > Dept. of Microbiology > Univ. of Birmingham at Alabama > "I am not a scientist, but I've seen one on T.V." > This discussion is interesting to me because of the results that we have found in the examination of cytokine expression in endomyocardial biopsies from human cardiac transplant recipients. Since they are immunosuppressed with cyclosporine and steroids, it is unusual to see much IL-2 production anywhere. But as they approach rejection, it seems that a subset of samples show a substantial increase in the expression of IL-4 and IL-10 just prior to the diagnosis of rejection, which is not what I would expect given some of the tenets of TH1/TH2 paradigm. They behave as if they are potentiating the response instead, which could happen if there is a shift in the composition or the type of signal transmitted by the target receptors. ============================================================================== James F. George, Ph.D. "Back off man, I'm a scientist" Department of Surgery Bill Murray in Ghostbusters University of Alabama at Birmingham 205-934-4261 voice txpljfg@uabcvsr.cvsr.uab.edu =============================================================================== From owner-immunology@net.bio.net Thu Dec 09 22:00:00 1993 Path: biosci!bcm!cs.utexas.edu!uunet!news.centerline.com!noc.near.net!delphi.bc.edu!bcvms.bc.edu!markmano From: markmano@bcvms.bc.edu Newsgroups: bionet.immunology Subject: expresion on viral-envelope Message-ID: <1993Dec10.164429.1@bcvms.bc.edu> Date: 10 Dec 93 20:44:29 GMT Organization: Boston College Lines: 13 NNTP-Posting-Host: bcvax2.bc.edu Dear netters I was asked to answer the following: can anybody help me with a protocol for expresion library where all the proteins are expressed as fusion proteins to one of the envelope proteins. I have heard a lecture form Gerg Winter three years ago in France where such a system was used to fish antibodies. Some body said that there might allready be a kit for it. I am not so much on the technical litrature of DNA. Thanks Ofer Markman, Boston College. From owner-immunology@net.bio.net Sun Dec 12 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!zib-berlin.de!math.fu-berlin.de!news.th-darmstadt.de!rbg.informatik.th-darmstadt.de!anh From: anh@rbg.informatik.th-darmstadt.de (Oliver Anh) Newsgroups: bionet.immunology Subject: Cell culture Date: 13 Dec 1993 17:13:59 GMT Organization: TU Darmstadt Lines: 14 Distribution: world Message-ID: <2ei7snINNmjv@rs18.hrz.th-darmstadt.de> NNTP-Posting-Host: rbhp75.rbg.informatik.th-darmstadt.de I, I'm a french student, 5. semester, actually in Germany I'm making a seminar ( student are listening ) of Immunology My subject is Cell Culture. I don't know what to say, what kind of experience is possible, and how to do it. Could someone give me some infos, Thanx in advance, Merry Xmas and Happy New Year. ------------------------------------------------------------------------------- / / Olivier ANH (ERAMUS Student) | 'I'm a poor lonesome student, \ X / anh@rbg.informatik.th-darmstadt.de | and I'm a long long 'way from home' ------------------------------------------------------------------------------- From owner-immunology@net.bio.net Sun Dec 12 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!cs.utexas.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!moe.ksu.ksu.edu!crcnis1.unl.edu!ns.ccsn.edu!earth.doane.edu!dkatz From: dkatz@earth.doane.edu Newsgroups: bionet.immunology Subject: lab hints Date: 13 Dec 93 15:01:14 CTS Organization: Doane College Lines: 19 Distribution: world Message-ID: <1993Dec13.150114.1@earth.doane.edu> Reply-To: self NNTP-Posting-Host: earth.doane.edu Since this is to be the first semester for me to teach immunology, I will probably be asking some questions of you tolerent immunologists this next semester. Please bear with me. I will be doing a lab with the course, and have come up with a couple of ideas. 1. One of the standard lab experiments is to immunize and follow the course of the antibody production. If we do this, it will have to be in mice, since we have not got the facility to handle larger animals. Has anyone ever thought about getting serum samples from rather large puppies as they undergo their puppy shots ,and following the antibody titers this way? 2. Another technique that is often used is having students isolate antibodies from serum samples. Off hand, does anyone know if it is very different to take antibodies out of colostrum or milk samples? 3. If anyone has any other ideas that are used in immuno. labs, I'd be very interested in speaking or emailing with you. Budgets are limited, but I want to give my students the best experience an old bacterial geneticist can give in immunology. Thanks for any help. Sue Katz, Biology, Doane College, Crete, NE. email at DKatz@Doane.edu. From owner-immunology@net.bio.net Sun Dec 12 22:00:00 1993 Path: biosci!bcm!cs.utexas.edu!sdd.hp.com!nigel.msen.com!emory!europa.eng.gtefsd.com!uunet!pipex!zaphod.crihan.fr!news.univ-rennes1.fr!roland.univ-rennes1.fr!nmenoux From: nmenoux@roland.univ-rennes1.fr ( Nicolas Menoux) Newsgroups: bionet.immunology Subject: ===== READ ME ===== Date: 13 Dec 1993 22:27:00 GMT Organization: Universite de Rennes 1, France Lines: 162 Message-ID: <2eiq7k$j03@news.univ-rennes1.fr> NNTP-Posting-Host: roland.univ-rennes1.fr X-Newsreader: TIN [version 1.2 PL0] Nicholas MENOUX 10, rue Michelet F35700 RENNES FRANCE Phone: (33)99368508 Fax: (33)99286957 Dec.13, 1993 Hello ! At present, I am looking for a job or a training period in immunology research programs. I am novice in that field because I have a master of science in Electrical Engineering and Computer Science but not in immunology. However, I am really interested in switching in that research field, especially in Tcells and MHC molecules researchs. I really want to work for researchers by designing and carrying out graphic tools systems with PCs or workstations like sparc, RS/6000,etc.. I am motivated to learn immunology technics and knowledge and help researchers in their works. I have a strong interest in Image (3D, 2D). I think I have a good Graphic interface background with numerous platforms like OpenLook, XWindow, GKS. Moreover, my educational background in harware could be a plus to develop some new devices in order to improve Human-Machine interface in immunology. As you can see, I have the research feeling and I am always fascinated in working for research. I got my Master degree in Computer Science and Electrical Engineering from Engineer School, Nantes University - FRANCE. I am quick at learning, hard working, results oriented and really creative. I think the combination of knowledges in both programming and Image with training and experience will enable me to make contribution to your laboratory. The following is what I am familiar with * C, C++, Fortran, Pascal and Visual-Basic * Sun OpenWindows, MS-Windows 3.1, X-WINDOW, GKS, ... * Unix SunOs, AIX, MS-Dos 6.0 * 3D Image, ray-tracing, ... * MS-Word, FrameMaker, Ventura-Publisher My resume is enclosed for your reference. Thank you for considering my qualifications for any potential position. Please call or e-mail me if I can provide any additional information. Sincerely, Nicholas MENOUX =============================================================================== NICHOLAS MENOUX Current address: 10, rue Michelet F35700 RENNES FRANCE Phone: (33)99368508 Fax: (33)99286957 E-mail: nmenoux@univ-rennes1.fr Date of birth: 01/25/69 Nationality: French Marital Status: Single Languages spoken: English (TOEFL 567), German, French EDUCATIONAL BACKGROUND ====================== 1990-1992 Three-year course in IRESTE (University of Nantes - Graduate School of Electrical Engineering and Computer Science) MS CS/EE : Diploma obtained in June 1992 1988-1989 Two-year preparation school in depth math & physics courses (intensive preparation for the competitive entrance examination giving access to advanced national schools of engineering) 1987 Baccalaureat C (French equivalent to the Higher Leaving Certificate, math and physics, with scientific orientation) ABILITIES ========= * Sense of Creativity * Resourcefulness and Adaptation Operating Systems: DOS, MS Windows, Unix, Aix, ... Graphic Interfaces: Windows, X-Window, GKS, Phigs, Openview, Sunview, ... Languages: C, C++, Pascal, SQL, Visual Basic, ... MicroP: 68000, 80x86, 6502, ... PROFESSIONAL BACKGROUND ======================= 1992 IBM France (Montpellier). Six-month industrial training period as a local area network administrator in the Advanced Techniques Group. I was responsible for 20 Xstations, 6 PS2 and 3 RS/6000 workstations under AIX 3.2 and TCP/IP protocols. I designed an Xwindow module in order to facilitate LAN administration. Moreover, I made a close study of external connections on Internet with X.25 protocols (IP encapsulated). 1992 Real-time application in VxWorks environment. I set a real-time speed control up in a distributed architecture with a Sun Sparc and two 68030 VME cards. The Sun workstation was used to design the speed graph (Sunview) and both VME cards to control the motor. 1992 I carried out a graphic program to assist researchers in spectrum analysis. (Atomic and Molecular Physics Department - University of Rennes - France) 1991 Two-month industrial training period with the S2HF research laboratory in Nantes. This laboratory works on Hyper-frequency for radar measurements. I made an easy and interactive interface to visualize 2D & 3D hyper-frequency images. 1991 Communication project selected by the Telesystems Institute of Paris. I presented my project in May 1991 in M.I.T and Boston Communication School. 1990 Industrial training period with La Telephonie Centrale (French Telephone Company which marketed Numeris(ISDN) telephone). I designed and carried out a local area network to facilitate interdepartment communication inside the company. I used Dbase III+ with DBcom option module and LAN servers. 1988-1989 I designed my own 1200 bps server at home on Apple //c. It was based on the modem card of the well-known french terminal : MINITEL. 1987 One-month training period with GRAVI Production (French company specialized in Image Synthesis and 3D animation). I made a program on Apple //c to preview 3D wire frames and a serial link to the main graphic server. 1984 I designed a video-game in 6502 machine code on Oric Atmos. It was selected by LORICIELS french video-game editor. 1983 I designed my first video-game "CANADA" on ORIC-1. It was commercialized by MICROLOGIC, a french video-game editor. MISCELLANEOUS ============= * Numerous travels all around the World (USA, Russia, European and Eastern countries, ...) * Participate in different school newspapers * Black & White photographs, Drawing * Squash From owner-immunology@net.bio.net Sun Dec 12 22:00:00 1993 Newsgroups: bionet.immunology Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!usenet.ins.cwru.edu!news.ecn.bgu.edu!willis1.cis.uab.edu!beagley.dom.uab.edu!user From: holland@gasmac.dom.uab.edu (Steve Holland) Subject: Re: lab hints Message-ID: Followup-To: bionet.immunology Sender: root@cis.uab.edu (Operator) Organization: UAB div GI References: <1993Dec13.150114.1@earth.doane.edu> Date: Mon, 13 Dec 1993 21:57:07 GMT Lines: 52 In article <1993Dec13.150114.1@earth.doane.edu>, dkatz@earth.doane.edu wrote: > > Since this is to be the first semester for me to teach immunology, I will > probably be asking some questions of you tolerent immunologists this next > semester. Please bear with me. I will be doing a lab with the course, and > have come up with a couple of ideas. > 1. One of the standard lab experiments is to immunize and follow the course > of the antibody production. If we do this, it will have to be in mice, since > we have not got the facility to handle larger animals. Has anyone ever thought > about getting serum samples from rather large puppies as they undergo their > puppy shots ,and following the antibody titers this way? An interesting idea. the problem will be getting antigen to plate for an ELISA. I suppose you could go with an Ouchterloney, but an ELISA is more current. You might look into donations from the company that makes the vaccines. They might also have some info on their results with antibody levels developed after vaccination. > 2. Another technique that is often used is having students isolate antibodies > from serum samples. Off hand, does anyone know if it is very different to take > antibodies out of colostrum or milk samples? Well, the technique to draw the sample will be different. An interesting aspect would be to look at colostrum vs milk antibody levels, colostrum is much higher. Local dairy farmers might be able to give you milk samples from just after vaccination to see what happens. One practical problem is eliminating the fat. a spin in a refrigerated ultracentrifuge works well. there is also a product called lipo-clean that may work on milk. It is great for ascites. Salivary antibody levels are another source of antibodies. More practical than stool antibody levels in humans under the circumstances. There was an article that described measuring stool antibodies in mice - you can extract IgA from the pellets in mice. > 3. If anyone has any other ideas that are used in immuno. labs, I'd be very > interested in speaking or emailing with you. Budgets are limited, but I want > to give my students the best experience an old bacterial geneticist can give in > immunology. How about skin transplants in mice across strains and in and out of offspring and F2's and the like? Could get some cell lines responsive to cytokines going and do some assays. weHI 279 grow without cytokine and are killed by interferon gamma. Maybe some IL-1 assays using thymocyte proliferation? best of luck with the course. Please post some of your plans. I would like to akeep notes on a low cost immunology lab experience for future teaching I will do. steve Holland From owner-immunology@net.bio.net Sun Dec 12 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!ulowell!europa.eng.gtefsd.com!howland.reston.ans.net!agate!msuinfo!netnews.upenn.edu!jkessler From: jkessler@mail.sas.upenn.edu (Jason A Kessler) Newsgroups: bionet.immunology Subject: Tolerence induction in B lymphocytes Date: 13 Dec 1993 18:46:37 GMT Organization: University of Pennsylvania, School of Arts and Sciences Lines: 12 Distribution: world Message-ID: <2eidad$rtp@netnews.upenn.edu> NNTP-Posting-Host: mail.sas.upenn.edu Hi. I am an undergraduate here at the University of Pennsylvania and am looking for current theories, views, etc. on the topic of self-non-self discrimination in the immature B cell population. Any information that anyone can provide me with would be greatly appreciated. I am especially curious if there are any parallels between T cell and B cell tolerence induction with respect to required environmental conditions (e.g. analagous factor in B cells differentiation to the thymus epithelial cells responsible for selection of T cell populations). Please email the responses to me directly. Thank you very much. jkessler@mail.sas.upenn.edu From owner-immunology@net.bio.net Mon Dec 13 22:00:00 1993 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!noc.near.net!delphi.bc.edu!bcvms.bc.edu!markmano From: markmano@bcvms.bc.edu Newsgroups: bionet.immunology Subject: SUMMARY: PHAGE DISPLAY Date: 13 Dec 93 19:40:28 EDT Organization: Boston College Lines: 166 Message-ID: <1993Dec13.194028.1@bcvms.bc.edu> NNTP-Posting-Host: bcvax2.bc.edu Thank you all for your replies on my question about phage display: I am enclosing a summary of the responses I have got so far: From: IN%"ajgeorge@rpms.ac.uk" 13-DEC-1993 11:49:45.99 To: IN%"markmano@bcvms.bc.edu" CC: Subj: phAge display Ofer Markman I enclose a few references re phage display systems. Hope they are of use. I do not know how to send messages to immunology bulletin board via Gopher so am sending direct to you.Any help on that much appreciated! The references are fairly eclectic, I have concentrated on non-antibody ones as they are less well known! Phage display systems are marketed by Pharmacia (for antibodies) and Promega. (there may be others). Barbas, C. F., III, Kang, A. S., Lerner, R. A. & Benkovic, S. J. (1991). Assembly of Combinatorial Antibody Libraries on Phage Surfaces: The Gene III Site. Proceedings of the National Academy of Sciences (U.S.A.), 88, 7978-7982. Breitling, F., Dubel, S., Seehaus, T., Klewinghaus, I. & Little, M. (1991). A Surface Expression Vector for Antibody Screening. Gene, 104, 147-153. Clackson, T., Hoogenboom, H. R., Griffiths, A. D. & Winter, G. (1991). Making Antibody Fragments Using Phage Display Libraries. Nature (London), 352, 624-628. Cwirla, S. E., Peters, E. A., Barrett, R. W. & Dower, W. J. (1990). Peptides on Phage: a Vast Library of Peptides for Identifying Ligands. Proceedings of the National Academy of Sciences (U.S.A.), 87, 6378-6382. Devlin, J. J., Panganiban, L. C. & Devlin, P. E. (1990). Random Peptide Libraries: a Source of Specific Protein Binding Molecules. Science, 249, 404-406. George, A. J. T. (1993). The Production of Antibodies Using Phage Display Libraries. In Monoclonal Antibodies, ed. M. A. Ritter, pp. in press. Cambridge: Cambridge University Press. Greenwood, J., Willis, A. E. & R.N.Perham (1991). Multiple Display of Foreign Peptides on a Filamentous Bacteriophage. Peptides from Plasmodium falciparum Circumsporozoite Protein as Antigens. Journal of Molecular Biology, 220, 821-827. Hammer, J., Takacs, B. & Sinigaglia, F. (1992). Identification of a Motif for HLA-DR1 Binding Peptides Using M13 Display Libraries. Journal of Experimental Medicine, 176, 1007-1013. Hawkins, R. E., Russell, S. J. & Winter, G. (1992). Selection of Phage Antibodies by Binding Affinity. Mimicking Affinity Maturation. Journal of Molecular Biology, 226, 889-896. Hoogenboom, H. R., Griffiths, A. D., Johnson, K. S., Chiswell, D. J., Hudson, P. & Winter, G. (1991). Multi-Subunit Proteins on the Surface of Filamentous Phage: Methodologies for Displaying Antibody (Fab) Heavy and Light Chains. Nucleic Acids Research, 19, 4133-4137. Hoogenboom, H. R. & Winter, G. (1992). By-Passing Immunisation. Human Antibodies from Synthetic Repertoires of Germline VH Gene Segments Rearranged in vitro. Journal Molecular Biology, 227, 381-388. Huse, W. D., Sastry, L., Iverson, S. A., Kang, A. S., Alting-Mees, M., Burton, D. R., Benkovic, S. J. & Lerner, R. A. (1989). Generation of a Large Combinatorial Library of the Immunoglobulin Repertoire in Phage Lambda. Science, 246, 1275-1281. Kang, A. S., Barbas, C. F., Janda, K. D., Benkovic, S. J. & Lerner, R. A. (1991). Linkage of Recognition and Replication Functions by Assembling Combinatorial Antibody Fab Libraries along Phage Surfaces. Proceedings of the National Academy of Sciences (U.S.A.), 88, 4363-4366. Lowman, H. B., Bass, S. H., Simpson, N. & Wells, J. A. (1991). Selecting High-Affinity Binding Proteins by Monovalent Phage Display. Biochemistry, 30, 10832-10838. Marks, J. D., Hoogenboom, H. R., Bonnert, T. P., McCafferty, J., Griffiths, A. D. & Winter, G. (1991). By-passing Immunization. Human Antibodies from V-gene Libraries Displayed in Phage. Journal of Molecular Biology, 222, 581-597. Marks, J. D., Hoogenboom, H. R., Griffiths, A. D. & Winter, G. (1992). Molecular Evolution of Proteins on Filamentous Phage. Mimicking the Strategy of the Immune System. Journal of Biological Chemistry, 267, 16007-16010. Oldenburg, K. R., Longanathan, D., Goldstein, I. J., Scholtz, P. G. & Gallop, M. A. (1992). Peptide Ligands for a Sugar-Binding Protein Isolated from a Random Peptide Library. Proceedings of the National Academy of Sciences (U.S.A.), 89, Roberts, B. L., Markland, W., Ley, A. C., Kent, R. B., White, D. W., Guterman, S. K. & Ladner, R. C. (1992). Directed Evolution of a Protein: Selection of Potent Neutrophil Elastase Inhibitors Displayed on M13 Fusion Phage. Proceedings of the National Academy of Sciences (U.S.A.), 89, 2429-2433. Schatz, P. J. (October 1993). Use of Peptide Libraries to Map the Substrate Specificity of a Peptide-Modifying Enzyme - A 13 Residue Consensus Peptide Specifies Biotinylation in Escherichia coli. Bio - Technology, 11, 1138-1143. Smith, G. P. (1985). Filamentous Fusion Phage: Novel Expression Vectors that Display Cloned Antigens on the Virion Surface. Science, 228, 1315-1317. Stephen, C. W. & Lane, D. P. (1992). Mutant Conformation of p53. Precise Epitope Mapping Using a Filamentous Phage Epitope Library. Journal of Molecular Biology, 225, 577-583. Tsunetsgu-Yokota, Y., Tatsumi, M., Robert, V., Devaux, C., Spire, B., Chermann, J. C. & Hirsch, I. (1991). Expression of an Immunogenic Region of HIV by a Filamentous Bacteriophage Vector. Gene, 99, 261-265. Good luck Andrew ****************************************************************************** Andrew J.T. George Department of Immunology Royal Postgraduate Medical School Hammersmith Hospital Du Cane Road London W12 0NN Tel 081 743 2030 ext 2089 From overseas +44 81 743 2030 ext 2089 Fax 081 749 8034 +44 81 749 8034 Email: JANET ajgeorge@uk.ac.rpms BITNET ajgeorge%uk.ac.rpms@ukacrl.bitnet INTERNET ajgeorge%rpms.ac.uk@nsfnet-relay.ac.uk UUCP ajgeorge%uk.ac.rpms@net.uu.uunet ****************************************************************************** From: IN%"J.Hexham@sheffield.ac.uk" "Mark Hexham]" 13-DEC-1993 07:58:09.47 To: IN%"markmano@bcvms.bc.edu" There are several systems around for doing this for a reveiw and lots of examples see Gene vol 23 I think where the whole issue is devoted to applications of this method. The two vectors I know of for this technique for antibodies are from Greg Winters lab sold as akit by Pharmacia (in UK anyway) and the pComb 3 vector available from Carlos Barbas or Dennis Burton at Scripps free for research purposes. ===================================================== Date: Sat, 11 Dec 1993 15:09:47 -0500 From: Angelo Gunasekera Organization: Princeton University Pharmacia sells a kit for peptide library work (specially designed to make single chain antibodies) Hope this helps Angelo From owner-immunology@net.bio.net Mon Dec 13 22:00:00 1993 Path: biosci!genpharm.com!dmoregan From: dmoregan@genpharm.com ("Donna Munoz O'Regan") Newsgroups: bionet.immunology Subject: Mouse IL-4 Date: 13 Dec 1993 16:33:38 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <199312140017.QAA17132@netcomsv.netcom.com> Subject: Time:4:06 PM OFFICE MEMO Mouse IL-4 Date:12.13.93 Hi, I was wondering has anyone injected mouse IL-4 into a mouse to stimulate an immune response and if so, 1) at what concentration did you use it and secondly, 2) what route of injection did you use? I'd appreciate any ideas you may have. Thanks in advance. Donna Munoz-O'Regan dmoregan@genpharm.com From owner-immunology@net.bio.net Mon Dec 13 22:00:00 1993 Path: biosci!CARROLL1.CC.EDU!lzett From: lzett@CARROLL1.CC.EDU (Leslie D Zetter) Newsgroups: bionet.immunology Subject: Subscribe Date: 13 Dec 1993 15:32:56 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <9312132332.AA26080@net.bio.net> SUBSCRIBE PETE BURROWS burrows@orion.cmc.uab.edu From owner-immunology@net.bio.net Mon Dec 13 22:00:00 1993 Path: biosci!bcm!cs.utexas.edu!uunet!caen!batcomputer!ghost.dsi.unimi.it!univ-lyon1.fr!news.imag.fr!ciril.fr!mouze From: mouze@ciril.fr (Marc Mouze-Amady) Newsgroups: bionet.immunology Subject: any experience with salivary cytokines? Date: 14 Dec 1993 09:21:13 GMT Organization: CIRIL, Nancy, France Lines: 16 Message-ID: <2ek0i9$rma@arcturus.ciril.fr> NNTP-Posting-Host: hp825.inrs.fr X-Newsreader: TIN [version 1.1 PL9] Hello, I am looking for references on cytokines excreted into saliva. Any help would be appreciated.Thanks in advance. Marc (mouze@inrs.fr). +=======================================================+ + Marc MOUZE-AMADY PhD + Voice : 33-83.50.20.00 + + I.N.R.S. + ext. 25.28 + + Environ. Physiol. dept. +========================+ + Occupational Physiol. lab. + Fax : 33-83.50.20.19 + + B.P. 27 + 33-83.50.20.97 + + F-54501 Vandoeuvre cedex +========================+ + FRANCE + E.mail: mouze@inrs.fr + +=======================================================+ ........................................................ From owner-immunology@net.bio.net Mon Dec 13 22:00:00 1993 Path: biosci!bloom-beacon.mit.edu!gatech!howland.reston.ans.net!pipex!zaphod.crihan.fr!jussieu.fr!univ-lyon1.fr!news.imag.fr!ciril.fr!mouze From: mouze@ciril.fr (Marc Mouze-Amady) Newsgroups: bionet.immunology Subject: any experience with salivary cytokines? Date: 14 Dec 1993 08:47:49 GMT Organization: CIRIL, Nancy, France Lines: 16 Message-ID: <2ejujl$e4q@arcturus.ciril.fr> NNTP-Posting-Host: hp825.inrs.fr X-Newsreader: TIN [version 1.1 PL9] Hello bionetters, I am looking for references on cytokines excreted into saliva. Any help would be appreciated.Thanks in advance. Marc. +=======================================================+ + Marc MOUZE-AMADY PhD + Voice : 33-83.50.20.00 + + I.N.R.S. + ext. 25.28 + + Environ. Physiol. dept. +========================+ + Occupational Physiol. lab. + Fax : 33-83.50.20.19 + + B.P. 27 + 33-83.50.20.97 + + F-54501 Vandoeuvre cedex +========================+ + FRANCE + E.mail: mouze@inrs.fr + +=======================================================+ ........................................................ From owner-immunology@net.bio.net Mon Dec 13 22:00:00 1993 Newsgroups: bionet.immunology Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!agate!ames!decwrl!decwrl!netcomsv!chiron.com!chiremv!green From: green@chiremv.chiron.com (Grant Green) Subject: Re: SUMMARY: PHAGE DISPLAY Message-ID: Sender: news@chiron.com Nntp-Posting-Host: chiremv Organization: Chiron Corporation References: <1993Dec13.194028.1@bcvms.bc.edu> Date: Tue, 14 Dec 1993 17:01:35 GMT Lines: 8 Let's not forget R.C. Ladner et al., U.S. Patent No. 5,223,409: "Directed Evolution of Novel Binding Proteins" -- @@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@ Grant Green |The brain is a wonderful organ: it starts working the moment green@chiron.com| you get up, and doesn't stop till you get to work. @@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@ From owner-immunology@net.bio.net Tue Dec 14 22:00:00 1993 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!pipex!zaphod.crihan.fr!jussieu.fr!univ-lyon1.fr!news.imag.fr!ciril.fr!mouze From: mouze@ciril.fr (Marc Mouze-Amady) Newsgroups: bionet.immunology Subject: any experience with salivary cytokines? Date: 15 Dec 1993 16:07:47 GMT Organization: CIRIL, Nancy, France Lines: 24 Message-ID: <2encoj$1i3@arcturus.ciril.fr> NNTP-Posting-Host: hp825.inrs.fr X-Newsreader: TIN [version 1.1 PL9] [ Article crossposted from sci.med.psychobiology ] [ Author was Marc Mouze-Amady ] [ Posted on 14 Dec 1993 09:25:28 GMT ] [ Article crossposted from bionet.immunology ] [ Author was Marc Mouze-Amady ] [ Posted on 14 Dec 1993 08:47:49 GMT ] Hello bionetters, I am looking for references on cytokines excreted into saliva. Any help would be appreciated.Thanks in advance. Marc. +=======================================================+ + Marc MOUZE-AMADY PhD + Voice : 33-83.50.20.00 + + I.N.R.S. + ext. 25.28 + + Environ. Physiol. dept. +========================+ + Occupational Physiol. lab. + Fax : 33-83.50.20.19 + + B.P. 27 + 33-83.50.20.97 + + F-54501 Vandoeuvre cedex +========================+ + FRANCE + E.mail: mouze@inrs.fr + +=======================================================+ ...................................................... From owner-immunology@net.bio.net Tue Dec 14 22:00:00 1993 Newsgroups: bionet.immunology Path: biosci!CS.Arizona.EDU!rainbow.cse.nau.edu!nauvax.ucc.nau.edu!alr From: alr@nauvax.ucc.nau.edu (LADY ANNA) Subject: Lupus Message-ID: <14DEC93.19293365@nauvax.ucc.nau.edu> Sender: news@rainbow.cse.nau.edu (Usenet News (system)) Nntp-Posting-Host: nauvax.ucc.nau.edu Organization: Northern Arizona University Date: Tue, 14 Dec 1993 19:29:33 GMT Lines: 12 Hi again. I read recently that they are putting together a new drug that may help lupus patients. I think it is called 714X or something to that effect. It consists of a shot in the leg at least once a day. I would really like any additional info anybody has on this. Having SLE, this is a rather important subject to me. Thanks. Please Email me or post here Anna Rogers alr@nauvax.ucc.nau.edu From owner-immunology@net.bio.net Tue Dec 14 22:00:00 1993 Path: biosci!genpharm.com!dmoregan From: dmoregan@genpharm.com ("Donna Munoz O'Regan") Newsgroups: bionet.immunology Subject: None Date: 15 Dec 1993 12:43:54 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <199312152042.MAA10470@netcomsv.netcom.com> NNTP-Posting-Host: net.bio.net Subject: Time:12:08 PM OFFICE MEMO None Date:12.15.93 Hi, I posted this query a few days ago, and I haven't received any replies, is it because no readers have done this procedure or is it because I didn't mail it properly to the immunology bulletin board. So here is my question: I was wondering has anyone injected mouse IL-4 into a mouse to stimulate an immune response and if so, 1) at what concentration, 2) volume, 3) what route of injection did you use? I'd appreciate any ideas and references you may have. Thanks in advance. Donna Munoz-O'Regan e-mail address: dmoregan@genpharm.com From owner-immunology@net.bio.net Wed Dec 15 22:00:00 1993 Newsgroups: bionet.immunology Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!usenet.ins.cwru.edu!news.ecn.bgu.edu!willis1.cis.uab.edu!right.dom.uab.edu!user From: holland@gasmac.dom.uab.edu (Steve Holland) Subject: Purifying Ig out of milk Message-ID: Followup-To: bionet.immunology Sender: root@cis.uab.edu (Operator) Organization: UAB Date: Thu, 16 Dec 1993 16:05:07 GMT Lines: 25 Recently someone posted a request for experiments that could be used for teaching. In the post was a request for info on extracting Ig from milk. George Mestecky is at UAB and has been working with IgA for years. I got this protocol verbally from one of his techs. When getting the milk, add PMSF and Soybean Trypsin inhibitor. Milk may then be frozen until processed. If processed immediatly, still add the antiproteinases. Perform all steps under refrigeration. Defat the milk by spinning at 10000 RPM for a while. Decasseinate the milk by bringing pH to 4 with acetic acid. Allow to stir for 1 hour, then spin hard and keep supernatant. Cassein will come down in with Ig in a slat cut, so it needs to come out with this acid precipitation. Perform 50% ammonium sulphate salt cut. Ig will be in precipitate. If you are just doing an ouchterlony all this may not be necessary. Steve Holland From owner-immunology@net.bio.net Wed Dec 15 22:00:00 1993 Path: biosci!genpharm.com!dmoregan From: dmoregan@genpharm.com ("Donna Munoz O'Regan") Newsgroups: bionet.immunology Subject: MOUSE IL-4 Date: 15 Dec 1993 16:14:52 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <199312160006.QAA24001@netcomsv.netcom.com> NNTP-Posting-Host: net.bio.net Subject: Time:12:08 PM OFFICE MEMO MOUSE IL-4 Date:12.15.93 Hi, I posted this query a few days ago, and I haven't received any replies, is it because no readers have done this procedure or is it because I didn't mail it properly to the immunology bulletin board. So here is my question: I was wondering has anyone injected mouse IL-4 into a mouse to stimulate an immune response and if so, 1) at what concentration, 2) volume, 3) what route of injection did you use? I'd appreciate any ideas and references you may have. Thanks in advance. Donna Munoz-O'Regan e-mail address: dmoregan@genpharm.com From owner-immunology@net.bio.net Thu Dec 16 22:00:00 1993 Newsgroups: bionet.immunology Path: biosci!bcm!cs.utexas.edu!uunet!EU.net!sun4nl!star.cs.vu.nl!balaena!mac133.bio.vu.nl!user From: cweijden@bio.vu.nl (C v/d Weijden) Subject: hyalonuridase Message-ID: Followup-To: bionet.immunology Sender: news@bio.vu.nl Organization: Mol.Micro, Vrije Uni., Amsterdam, NL Date: Fri, 17 Dec 1993 16:08:25 GMT Lines: 9 I am interested in an assay for hyalonuridase, preferably a micro-assay. Does somebody know such an assay. Please E-mail me this: PHJ.van_dalen.omb.acta@med.vu.nl -- Ph.J van Dalen Oral Microbiology Medical Faculty Free University, Amsterdam From owner-immunology@net.bio.net Fri Dec 17 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!agate!usenet.ins.cwru.edu!magnus.acs.ohio-state.edu!gchacko From: gchacko@magnus.acs.ohio-state.edu (George W Chacko) Newsgroups: bionet.immunology Subject: 41H16 Hybridoma Date: 18 Dec 1993 21:48:32 GMT Organization: The Ohio State University Lines: 8 Distribution: world Message-ID: <2evtrg$p90@charm.magnus.acs.ohio-state.edu> NNTP-Posting-Host: bottom.magnus.acs.ohio-state.edu Does anyone have information on the availability of mab 41H16 reactive against human FcgammaRII? It was raised in the laboratory of Theodore Zipf and I was told that the hybridoma is presently the subject of a legal dispute. George Chacko From owner-immunology@net.bio.net Mon Dec 20 22:00:00 1993 Path: biosci!daresbury!s-crim1!mbbhm From: mbbhm@s-crim1.dl.ac.uk (B.M. Meehan) Newsgroups: bionet.immunology Subject: Request for field sera Date: 21 Dec 1993 11:35:44 GMT Organization: SERC Daresbury Lab, Warrington, U.K. Lines: 29 Distribution: bionet Message-ID: <2f6n2g$1mk@mserv1.dl.ac.uk> NNTP-Posting-Host: s-crim1.dl.ac.uk Summary: Request for positive field sera Keywords: coronavirus lentivirus X-Newsreader: TIN [version 1.2 PL2] Hi, I am presently working on the production of baculovirus expressed animal virus antigens for use in ELISA diagnostics. The viruses we are working on are the coronaviruses transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV) in addition to the lentiviruses maedi visna (MVV) and caprine arthritis encephalitis virus (CAEV). The main problem that we are having is the availability of positive field sera to these viruses. We only have experimental sera for evaluation purposes. Because of this I would be grateful for any sources of positive field sera for these diseases. We would be able to provide an import licence prior to dispatch and cover postage costs. Any source of help would be greatly appreciated Brian Meehan Brian Meehan Dept. of Veterinary Science The Queen's University of Belfast Stormont Belfast BT4 3SD uk tele uk (0)232 525643 fax uk (0)232 761662 E-mail MBBHM@seqnet.dl.ac.uk From owner-immunology@net.bio.net Mon Dec 20 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!warwick!pipex!howland.reston.ans.net!gatech!isye!jloden From: jloden@isye.gatech.edu (Janice Loden) Newsgroups: bionet.immunology Subject: Low Immune System Date: 21 Dec 1993 20:15:15 GMT Organization: Georgia Institute of Technology Lines: 23 Message-ID: <2f7lgj$9if@news.gatech.edu> NNTP-Posting-Host: isye.isye.gatech.edu Hi! I am new to this newsgroup, but I hope that someone can help answer my question(s). I have a 5 year old son, Glenn. 2 1/2 years ago I was told that Glenn had a low immune system and might need these treatments (intraveniously) over a 1 or 2 year period. In the meantime the doctor took blood and looked at the T's. Two of them were low. THey froze this blood, gave him all of his immunizations (again), and took blood 6 weeks later. THey then sent the frozen blood along with the newly taken blood to a lab to check his T's again. After checking this they gave him a pnemacockal (spelling is wrong) vaccine. Since birth he has had pnemonia 4 times. After getting this vaccine, he has been fine, until this week. His left lung is not looking good. Does anybody out there have any information on the T's in blood system and how they work? He has had an AIDS test, it was negative. *********************************************************************** * Janice Loden * * * * "Life is a ceremony, * * (404) 894-9317, fax (404) 894-2301 * the universe is alive, * * * I am honored to be a * * jloden@isye.gatech.edu * part of it" * * * *********************************************************************** From owner-immunology@net.bio.net Tue Dec 21 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!warwick!uknet!EU.net!howland.reston.ans.net!cs.utexas.edu!geraldo.cc.utexas.edu!utxvms.cc.utexas.edu!nigel From: nigel@utxvms.cc.utexas.edu Newsgroups: bionet.immunology Subject: L6 L8 cDNA library Date: 22 Dec 93 11:41:11 CST Organization: University of Texas @ Austin Lines: 16 Message-ID: <1993Dec22.114111.1@utxvms.cc.utexas.edu> NNTP-Posting-Host: bisque.cc.utexas.edu This is a search for a friend. I am looking for an L8 or L6 rat muscle cell cDNA expression library. The library should have been made from cells just before or during the fusion to form muscle cells. Does anyone have such a library? I know that this is a fantastically specific request but who knows? Please email me at Nigel@UTXVMS.cc.utexas.edu. Thanks Nigel Atkinson Asst Prof Zoology Univ. Texas at Austin Nigel@UTXVMS.cc.utexas.edu From owner-immunology@net.bio.net Wed Dec 22 22:00:00 1993 Path: biosci!dwe.csiro.au!Mark.Bradley From: Mark.Bradley@dwe.csiro.au (Mark Bradley) Newsgroups: bionet.immunology Subject: Salmonella recomb. Vaccines Date: 16 Dec 1993 15:27:05 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <199312162329.KAA08799@artemis.cbr.dwe.csiro.au> I am interested in exploring this technology and would like to be pointed to any recent reviews on the area of salmonella recombinant vaccines. Thanks in advance *********************************************************** Dr Mark Bradley Cooperative Research Centre for the Biological Control of Vertebrate Pest Populations CSIRO, Division of Wildlife & Ecology PO Box 84 Lyneham, Canberra, ACT, 2602 AUSTRALIA Fax: (06) 242 9242 Phone: (06) 2421733 email:mpb@cbr.dwe.csiro.au ********************************************************** From owner-immunology@net.bio.net Wed Dec 22 22:00:00 1993 Newsgroups: bionet.immunology Path: biosci!daresbury!doc.ic.ac.uk!agate!howland.reston.ans.net!vixen.cso.uiuc.edu!moe.ksu.ksu.edu!osuunx.ucc.okstate.edu!vms.ucc.okstate.edu!zeljkos From: zeljkos@vms.ucc.okstate.edu Subject: help ponceau staining Message-ID: <1993Dec22.231023.1@vms.ucc.okstate.edu> Lines: 13 Sender: news@osuunx.ucc.okstate.edu (USENET News System) Nntp-Posting-Host: vms.ucc.okstate.edu Organization: Oklahoma State University Computer Center Date: Thu, 23 Dec 1993 05:10:23 GMT I am having troubles using Ponceau S solution for temporary staining of western blots. I am definitely sure that my proteins were transferred to the membrane (rainbow marker confirms that) and in the satisfactory quantity. The Ponceau S (Sigma) solution was always prepared fresh according to Current Protocols in Mol. Biol., what means: 0.5g of Ponceau S dissolved in 1ml of acetic acid and diluted to 100ml with ddH20. The membrane used were PVDF either from Sigma or Biorad. I have tried with various incubation times from 5 to 30 minutes. Destining was under stream of the ddH20. Directly after semi dry transfer, the membrane was rinsed with PBS (50mM KP, 100mM NaCl) and immersed into Ponceau S solution. PLEASE DO SOMEONE HAS IDEA WHAT WAS WRONG. Thanks Zeljko. From owner-immunology@net.bio.net Wed Dec 22 22:00:00 1993 Path: biosci!hubcap!darwin.sura.net!tulane!wupost!waikato!canterbury.ac.nz!chmeds.ac.nz!arae From: arae@chmeds.ac.nz Newsgroups: bionet.immunology Subject: Message-ID: <1993Dec24.113305.392@chmeds.ac.nz> Date: 24 Dec 93 11:33:05 +1200 Lines: 13 Our Immunology Unit is interested in establishing a confirmatory Western Blot assay for suspected Scl-70 antibody positive samples. Can anybody help me identify a good source of the Scl-70 antigen? Any help would be appreciated. I can be e-mailed on AaronR@garbo.uwasa.fi or ARae@bliss.chmeds.ac.nz or AaronR%chs_path@chhlth.govt.nz Cheers Aaron From owner-immunology@net.bio.net Wed Dec 29 22:00:00 1993 Newsgroups: bionet.immunology Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!newsrelay.iastate.edu!news.iastate.edu!kharkins From: kharkins@iastate.edu (Kristi R Harkins) Subject: Hybridoma Training Message-ID: Sender: news@news.iastate.edu (USENET News System) Organization: Iowa State University, Ames, IA Distribution: usa Date: Thu, 30 Dec 1993 21:57:58 GMT Lines: 20 I am interested in attending a training short course(s) which covers techniques used in Hybridoma Monoclonal Antibody Production. I am interested in both basic techniques training, as well as up-to-date training on innoculation regimes vs. antibody specificity/affinity, alternative antibody designs (anti-rat, anti-human), large scale antibody production (bioreactor), etc... Please send course info via email to kharkins@iastate.edu or by mail to: Dr. Kristi Harkins Iowa State University 1104 Molecular Biology Ames, IA 50011 -- ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ Kristi R. Harkins, Lab Director <> Office: 515/294-2472 Cell & Hybridoma Facility <> Lab: 515/294-8504 Iowa State University <> FAX: 515/294-0453 From owner-immunology@net.bio.net Wed Dec 29 22:00:00 1993 Newsgroups: bionet.immunology Path: biosci!daresbury!doc.ic.ac.uk!warwick!pipex!uunet!yeshua.marcam.com!zip.eecs.umich.edu!caen!msuinfo!netnews.upenn.edu!cronkite.ocis.temple.edu!VM.TEMPLE.EDU!RAPHAEL From: RAPHAEL@VM.TEMPLE.EDU Subject: Re: familial granulomatosis Message-ID: <16CB31166D.RAPHAEL@VM.TEMPLE.EDU> Sender: news@cronkite.ocis.temple.edu (NetWork News (readnews)) Nntp-Posting-Host: vm.temple.edu Organization: Temple University Date: Thu, 30 Dec 1993 00:57:52 GMT Lines: 12 Organization: Temple University Keywords: granuloma X-Newsreader: NNR/VM S_1.3.2 I am investigating the syndrome of familial granulomatous arthritis, uveitis and rash. This is the only known example, in humans, of a multisystem, granulomatous inflammatory condition inherited in an autosomal dominant manner. I would like to hear from anyone who may care/investigate such patients and/or has an interest in the basic immunology of granuloma formation. My research group is in the midst of a linkage analysis to try and isolate the putative gene chromosomal location and a study to better define the immunobiology of the condition. From owner-immunology@net.bio.net Thu Dec 30 22:00:00 1993 Path: biosci!bcm!cs.utexas.edu!usc!newshub.sdsu.edu!sunstroke!frohwer From: frohwer@sunstroke.sdsu.edu (Forest Rohwer) Newsgroups: bionet.immunology Subject: genes and cytokines Date: 31 Dec 1993 21:54:38 GMT Organization: San Diego State University, College of Sciences Lines: 8 Message-ID: <2g272u$q78@pandora.sdsu.edu> NNTP-Posting-Host: sunstroke.sdsu.edu X-Newsreader: TIN [version 1.2 PL0] Is anyone out there familiar with studies in which cells (i.e. T cells) were stimulated with a cytokine (i.e. IL-2) and then specific responding genes are isolated. I imagine that this could be done by subtractive hybridization and am thinking about trying it with immortalized T cells and IL-2, therefore I would be interested in any previous studies or ideas on this approach. Please feel free to E-mail me directly at frohwer@sunstroke.edu or through this bullentin board. Thanks in advance.