From owner-immuno@hgmp.mrc.ac.uk  Thu Jun  1 10:17:01 2000
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From: "Mehdi Alimadadi" <mehdia@interchange.ubc.ca>
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Dear Ad,

Is there any special reason for you to use cryo sections?  I mean did you
use actually frozen sections with microtome or cryosections with cryostat?
In which one do you believe the antigens remains better and intact?

A.M.







From owner-immuno@hgmp.mrc.ac.uk  Sat Jun  3 05:50:36 2000
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From: "Len" <rabeez@ihug.co.nz>
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Subject: case study on x-linked agammaglobulinemia
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Hi every one, I am looking for a case study on Bruton's type
agammaglobulinemia, any advice on where to look would be appreciated, or
even basics on a case would be great - gender, age, symptoms, lab data,
treatment.  Even a point in the right direction of where to look on the web
would be good..I tried medscape / medline, but they only had extracts and
the like, no whole cases..

Thanks in advance, Lenny





From owner-immuno@hgmp.mrc.ac.uk  Sun Jun  4 11:15:03 2000
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begin 666 freenews.netfront.net.iaf
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From owner-immuno@hgmp.mrc.ac.uk  Sun Jun  4 15:29:59 2000
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II International Conference
Heat shock proteins in Immune Response
Farmington, Connecticut, USA
October 8-12, 2000 

Topics to be discussed at the conference will include:
·       HSP-peptide complexes as vaccines against cancers and infectious
agents, including results from completed and ongoing clinical trials.
·       Structural insights into HSP-peptide interaction.
·       Evolution of immunological properties of HSPs.
·       Role of HSPs in antigen presentation by MHC molecules.
·       Role of HSPs in innate immunity.
·       HSPs as Immunomodulators and as targets of autoimmune response.

Confirmed speakers at the Conference are:  R. Amato (Houston), P.
Anderson (Boston), S. Basu (Farmington), R. Binder (Farmington),
Calderwood (Boston), R. DeSantis (Naploi), D. Forsdyke (Ontario), M.
Heike (Mainz), L. Hightower (Storrs), R. Kiessling (Stockholm), H. Kolb
(Dusseldorf), Z. Li (Farmington), A. Ménoret (Farmington), C. Nicchitta
(Durham), E. Repasky (Buffalo), S. Sastry (New York), N. Sato (Sapporo),
H. Schild (Tubingen), T. Spies (Seattle), J. Subjeck (Buffalo), H. Udono
(Nagasaki), W. van Eden (Utrecht), R. Vile (Rochester), A. von Bonin
(Hamburg), R. Young (Boston).

The deadline for registration and for submission of abstracts is July
15, 2000.  Student and post-doctoral travel grants will be awarded based
on the scientific quality of the submitted abstracts. This conference is
being sponsored by the Cell Stress Society, Antigenics Inc., Elan Corp.,
Immunex Corp.,and  Zeneca Corp.  For further information and details,
please contact:

Further information is available at our new web site: 
http://www.hspimmunity.com

Antoine Menoret




From owner-immuno@hgmp.mrc.ac.uk  Sun Jun  4 15:32:07 2000
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II International Conference
Heat shock proteins in Immune Response
Farmington, Connecticut, USA
October 8-12, 2000 

Topics to be discussed at the conference will include:
·       HSP-peptide complexes as vaccines against cancers and infectious
agents, including results from completed and ongoing clinical trials.
·       Structural insights into HSP-peptide interaction.
·       Evolution of immunological properties of HSPs.
·       Role of HSPs in antigen presentation by MHC molecules.
·       Role of HSPs in innate immunity.
·       HSPs as Immunomodulators and as targets of autoimmune response.

Confirmed speakers at the Conference are:  R. Amato (Houston), P.
Anderson (Boston), S. Basu (Farmington), R. Binder (Farmington),
Calderwood (Boston), R. DeSantis (Naploi), D. Forsdyke (Ontario), M.
Heike (Mainz), L. Hightower (Storrs), R. Kiessling (Stockholm), H. Kolb
(Dusseldorf), Z. Li (Farmington), A. Ménoret (Farmington), C. Nicchitta
(Durham), E. Repasky (Buffalo), S. Sastry (New York), N. Sato (Sapporo),
H. Schild (Tubingen), T. Spies (Seattle), J. Subjeck (Buffalo), H. Udono
(Nagasaki), W. van Eden (Utrecht), R. Vile (Rochester), A. von Bonin
(Hamburg), R. Young (Boston).

The deadline for registration and for submission of abstracts is July
15, 2000.  Student and post-doctoral travel grants will be awarded based
on the scientific quality of the submitted abstracts. This conference is
being sponsored by the Cell Stress Society, Antigenics Inc., Elan Corp.,
Immunex Corp.,and  Zeneca Corp.  For further information and details,
please contact:

Further information is available at our new web site: 
http://www.hspimmunity.com

Antoine Menoret




From owner-immuno@hgmp.mrc.ac.uk  Sun Jun  4 23:31:01 2000
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From: infoonthem.2@russia.com
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From owner-immuno@hgmp.mrc.ac.uk  Tue Jun  6 08:58:23 2000
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Message-ID: <393CAE59.4FCB7DBB@pharma.ethz.ch>
Date: Tue, 06 Jun 2000 09:55:05 +0200
From: Antoine Logean <alogean@pharma.ethz.ch>
Organization: ETHZ D-PAR
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How can I get a list of all human cytosolic proteins that have been
caracterized by crystatlisation or NMR
methods ?

thank you for your help




From owner-immuno@hgmp.mrc.ac.uk  Tue Jun  6 21:45:16 2000
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From: Bernd Wollscheid <wollscheid@immunbio.mpg.de>
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Subject: Boxit-Database for Molecular Biology Laboratories
Date: Tue, 06 Jun 2000 22:39:45 +0100
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> This message is in MIME format. Since your mail reader does not understand
this format, some or all of this message may not be legible.

--MS_Mac_OE_3043175986_16549884_MIME_Part
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--MS_Mac_OE_3043175986_16549884_MIME_Part
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<HTML>
<HEAD>
<TITLE>Boxit-Database for Molecular Biology Laboratories</TITLE>
</HEAD>
<BODY>
http://www.boxit-labstorage.de/ <BR>
<BR>
To all Labscientists,<BR>
<BR>
=80 Did someone recently leave your lab? <BR>
<BR>
=80<B> Can=B4t find things anymore? <BR>
</B><BR>
=80 Have you lost the information about the contents of a tube or where it is=
 stored? <BR>
<BR>
=80 Are you sick of spending time searching in folders and labbooks or at -20=
=B0C for a plasmid? <BR>
<BR>
=80 Are you and your co-workers wasting time and money to recreate what you c=
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From owner-immuno@hgmp.mrc.ac.uk  Tue Jun  6 22:16:04 2000
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From: "Theophilus Samuels" <theophilus.samuels@btinternet.com>
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Subject: 'Mistaken self' - study by van Noort et al.
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Has anyone read the paper published by Johannes M. van Noort et al. in the
Journal of Immunology (Vol. 105 p46-57, 2000) describing the possible role
of [alpha] B-crystallin in Multiple Sclerosis? If so, any comments?

T.L.S.







From owner-immuno@hgmp.mrc.ac.uk  Thu Jun  8 15:59:27 2000
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I'm currently using Molecular Device's SoftmaxPro ELISA plate reader
software to read ELISA plates and was wondering if I could get
comments/suggestions/recommendations on other ELISA plate reader
software?

-Martin




From owner-immuno@hgmp.mrc.ac.uk  Thu Jun  8 17:15:11 2000
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Hi, you may want to check out this free Elisa reader software:

http://members.tripod.com/~gestur/programs/titri.htm

However, I have not used it and I don't know if that is what you are
looking for. It is free, though.

Oswaldo



Naomi Kautz wrote:
> 
> I'm currently using Molecular Device's SoftmaxPro ELISA plate reader
> software to read ELISA plates and was wondering if I could get
> comments/suggestions/recommendations on other ELISA plate reader
> software?




From owner-immuno@hgmp.mrc.ac.uk  Fri Jun  9 01:52:36 2000
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From: lyndal@deakin.edu.au (Lyndal Kerr)
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Subject: Problems with Screening hybridoma's
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I am having problems with some Mab's we have produced and would greatly
appreciate any advice -

We immunised Balb/C mice with a Pinpoint (Promega) bacterial fusion
protein.  Prior to fusion, immune sera was tested with both the pinpoint
fusion protein and a GST fusion of the same protein.  End point titres
were excellent using both antigens, suggesting a good immune response to
our protein of interest.  Performed fusion and used the pinpoint fusion
protein to screen supernatants.  Positives were selected then
re-screened with the GST fusion protein to identify clones secreting
antibodies specific for our antigen and not the pinpoint tag.  Two lines
were pursued (2 rounds of single cell cloning - all OK - isotyped as IgM
Kappa) and supernants collected for use in westerns and
immunohistochemistry.  When titering the Mab by ELISA we used the
purified pinpoint fusion protein, GST fusion protein and the cleaved
(GST) protein, including GST alone as a negative control.  Both
supernatants from each line showed strong immunoreactivity with all
antigens including the GST -ve controls (all other controls - OK).  This
is bizarre since the GST fusion protein was not used to immunise mice.
We have also performed Western and Dot Blotting with the same
supernatants using the antigens listed above and obtained completely
different results (Westerns - weakly detecting GST but none of the other
antigens; Dot Blots - weakly detecting all GST contructs but strong
reactivity with the pinpoint fusion protein).  Immunohistochemistry
using the Mab supernatants has localised our protein to a specific
region in the brain however no staining is seen in any region of the
brain using anti-GST!

I know this is rather long-winded but I would much appreciate any idea's
- we are banging our heads against the wall!!!

Thanks in advance

Lyndal Kerr
lyndal@deakin.edu.au


---




From owner-immuno@hgmp.mrc.ac.uk  Fri Jun  9 13:21:47 2000
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From: Mike Clark <mrc7@cam.ac.uk>
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Subject: Re: Problems with Screening hybridoma's
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In article <394043D0.94118DC3@deakin.edu.au>, Lyndal Kerr
<URL:mailto:lyndal@deakin.edu.au> wrote:
> I am having problems with some Mab's we have produced and would greatly
> appreciate any advice -
> 
> We immunised Balb/C mice with a Pinpoint (Promega) bacterial fusion
> protein.  Prior to fusion, immune sera was tested with both the pinpoint
> fusion protein and a GST fusion of the same protein.  End point titres
> were excellent using both antigens, suggesting a good immune response to
> our protein of interest.  Performed fusion and used the pinpoint fusion
> protein to screen supernatants.  Positives were selected then
> re-screened with the GST fusion protein to identify clones secreting
> antibodies specific for our antigen and not the pinpoint tag.  Two lines
> were pursued (2 rounds of single cell cloning - all OK - isotyped as IgM
> Kappa) and supernants collected for use in westerns and
> immunohistochemistry.  When titering the Mab by ELISA we used the
> purified pinpoint fusion protein, GST fusion protein and the cleaved
> (GST) protein, including GST alone as a negative control.  Both
> supernatants from each line showed strong immunoreactivity with all
> antigens including the GST -ve controls (all other controls - OK).  This
> is bizarre since the GST fusion protein was not used to immunise mice.
> We have also performed Western and Dot Blotting with the same
> supernatants using the antigens listed above and obtained completely
> different results (Westerns - weakly detecting GST but none of the other
> antigens; Dot Blots - weakly detecting all GST contructs but strong
> reactivity with the pinpoint fusion protein).  Immunohistochemistry
> using the Mab supernatants has localised our protein to a specific
> region in the brain however no staining is seen in any region of the
> brain using anti-GST!
> 
> I know this is rather long-winded but I would much appreciate any idea's
> - we are banging our heads against the wall!!!
> 
> Thanks in advance
> 
> Lyndal Kerr
> lyndal@deakin.edu.au
> 

Hi Lyndal,

This is not an unusual occurrence in making hybridomas. The mistake is in
thinking that you only get back monoclonal antibodies specific for the
immunising antigen. Immunisation helps to increase the probability of
getting the antibody you want but isn't an absolute condition.

I learnt a similar lesson in the early days of hybridomas when I was a PhD
student in Cesar Milsteins laboratory. I had immunised rats with mouse
immunoglobulin with the intention of making some useful reagents for
screening mouse monoclonal antibodies. I carried out the fusion and then
screened the hybridoma supernatants on SRBC coupled with mouse Ig. This was
a convenient screening system as I could look for agglutination and lysis
just as used by Kohler and Milstein for Sp1 in their 1975 Nature paper.

What I ended up with was some of the first rat monoclonal antibodies
specific for SRBC (not mouse Ig!) even though SRBC was not an immunogen.

>From phage work we that numerous antibody specificities can be obtained
from naive libraries without any need to immunise. Immunisation increases
the probability of success but is no guarantee.

In your case I suggest you need to screen more hybridomas and to include
the negative controls at an earlier stage. The fact that your antibodies
are IgM may be indicative that they are part of a primary repertoire not
selected on your immunising antigen, i.e. no class-switching to IgG has
taken place. You conduct your assays using IgG specific reagents to avoid
detecting IgM although this might restrict your selection too much if no
IgG antibodies have been made.

P.S. [ Don't forget August 2000 is the 25th Anniversary of the Kohler and
Milstein Nature paper. There is a special issue of Immunology Today in
preparation.]

Mike Clark,                        <URL:http://www.path.cam.ac.uk/~mrc7/>
-- 
 o/ \\    //            ||  ,_ o   M.R. Clark, PhD. Division of Immunology
<\__,\\  //   __o       || /  /\,  Cambridge University, Dept. Pathology
 ">    ||   _`\<,_    //  \\ \> |  Tennis Court Rd., Cambridge CB2 1QP
  `    ||  (_)/ (_)  //    \\ \_   Tel.+44 1223 333705  Fax.+44 1223 333875




From owner-immuno@hgmp.mrc.ac.uk  Fri Jun  9 15:02:45 2000
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From: "Chong Wai Yin( Zhang Weixian)" <wychong@singnet.com.sg>
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Hi, I like to ask a question on cell wall synthesis. Is the
penicillin-binding protein of bacteria
conserve? As in e.g.. the pbp in S.aureus is different from that in say....
bacillus, or even Staphylococci spp.?

Kelvin Chong
-- For Nature, heartless, witless Nature
   Will neither know nor care.
   And from my pillow, looking forth by light
   Of moon or favouring stars, I could behold
   The antechapel where the statue stood
   Of Newton with his prism and silent face,
   The marble index of a mind for ever
   Voyaging through strange seas of Thought, alone. --





From owner-immuno@hgmp.mrc.ac.uk  Sat Jun 10 00:42:14 2000
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From: "Gareth Griffiths" <gareth.griffiths1@virgin.net>
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I am trying to find a supplier of reporter antibody conjugates against
marmoset immunoglobulins of all classes. I realise that this species is not
big in immunological circles but I wondered if any research groups have made
any of these reagents.

Any help would be much appreciated,

Thank you

Gareth Griffiths





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---




From owner-immuno@hgmp.mrc.ac.uk  Sat Jun 10 22:16:45 2000
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From: "Karine Crozat" <Karine.Crozat@wanadoo.fr>
X-Newsgroups: bionet.immunology
Subject: Re: immunostimulation
Date: Sat, 10 Jun 2000 22:41:32 +0200
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Oui, j'ai plein d'idées mais je ne comprends la "nature" du test d'
immunostimulation. Quels sont les éléments "cibles" à étudier?

A bientôt, en attendant de plus amples informations...

Francis Beauvais <beauvais@netcourrier.com> a écrit dans le message :
393114AE.BDB67A45@netcourrier.com...
>
> Dear netters,
>
> Have you some idea for a in vitro test for immunostimulation ?
>
> Thanks
>
> beauvais@worldnet.fr
>
>
>





From owner-immuno@hgmp.mrc.ac.uk  Sat Jun 10 22:16:47 2000
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I have been studying Immunology at the Luminy University in Marseille
(France) this year and I am looking for a practice period on an Immunology
laboratory in order to know more about manipulations and methods.
My degree corresponds to 4 years at a French University.

If you are not interested about my supply, could you please spread it by
word of mouth ?

Thank you for your help.





From owner-immuno@hgmp.mrc.ac.uk  Tue Jun 13 10:27:38 2000
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Why don't you do a homology search?

There are, by the way, several PBP's in each organism, with different Mws, and
(I'd assume) different primary sequence, but these groups are very probably
conserved in themselves

"Chong Wai Yin( Zhang Weixian)" wrote:

> Hi, I like to ask a question on cell wall synthesis. Is the
> penicillin-binding protein of bacteria
> conserve? As in e.g.. the pbp in S.aureus is different from that in say....
> bacillus, or even Staphylococci spp.?
>
> Kelvin Chong
> -- For Nature, heartless, witless Nature
>    Will neither know nor care.
>    And from my pillow, looking forth by light
>    Of moon or favouring stars, I could behold
>    The antechapel where the statue stood
>    Of Newton with his prism and silent face,
>    The marble index of a mind for ever
>    Voyaging through strange seas of Thought, alone. --

--
Susanne Rohrer
Institute of Medical Microbiology
University of Zurich
Switzerland

srohrer at immv dot unizh dot ch





From owner-immuno@hgmp.mrc.ac.uk  Tue Jun 13 12:18:03 2000
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From: wind@biobase.dk ("Troels Wind")
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Hi all,

I just finished mapping, via alanine-scanning, the epitopes recognised by a
few murine monoclonals raised against a human protein. To my surprise, I
find that the functional epitopes for these antibodies, i.e. those residues
in the antigen that when mutated to alanine reduces the affinity more than
3-fold,  are conserved between the human and the murine version of the
protein.

One explanation would be that residues close to the epitope, that are not
conserved, 'covers' the epitope in the mouse antigen but not in the human
antigen.

Could this be the case and have any of you heard of similar observations ?

I do not know yet if the antibodies actually binds the mouse antigen, but it
will be tested soon.


Thank you in advance,
Troels


Troels Wind, Ph.D.
Laboratory of Cellular Protein Science
Department of Molecular and Structural Biology
University of Aarhus
Gustav Wieds Vej 10C
8000 Aarhus C
Denmark

Phone: (+45) 8942 5079
Fax: (+45) 8612 3178

"Only two things are infinite, the universe and
human stupidity, and I'm not sure about the former."
                                                   -Albert Einstein


---




From owner-immuno@hgmp.mrc.ac.uk  Thu Jun 15 16:10:12 2000
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From: nan@inflam.lu.se
X-Newsgroups: bionet.immunology
Subject: isotype switch in established B cell hybridoma
Date: Thu, 15 Jun 2000 14:59:47 GMT
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Dear friends,

We are interested in getting the isotype switch variants of
an established, stable B-cell hybridoma. Now, the hybridoma
secretes IgG1 antibodies. I would like to use gamma interferon
with the hybridoma to get the IgG2a antibodies.
Is there any established protocol available or possible
references? Any lead will be highly useful.

With regards,
K.S.Nandakumar


Sent via Deja.com http://www.deja.com/
Before you buy.




From owner-immuno@hgmp.mrc.ac.uk  Thu Jun 15 16:50:04 2000
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From: "JEAN-CHRISTIAN LECHIEN" <jeanlechien@swing.be>
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Subject: SITE DE LA MALADIE DE BEHCET
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Bonjour à tous,
Le site de l4association de Behcet est :
http://perso.wanadoo.fr/boiteau.bruno/
mon mail
jeanlechien@swind.be
Le but de l'association est de rassembler le plus grand nombre de patients
affin de les aider, eux ainsi que leurs familles et de mieux faire connaître
aux médecins cette pathologie.
Si vous connaissez une personne qui est atteint du syndrome de Behcet,
n'hésitez pas à me contacter.
Merçi d'avance.
Jean-Christian Lechien





From owner-immuno@hgmp.mrc.ac.uk  Fri Jun 16 16:22:58 2000
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I will appreciate if somebody in there that uses the ELISA kits Predicta
for cytokines (from Genzyme), is so kind to e-mail me (or fax) the
protocols for IFN-g, IL-2, IL-4 and IL-10. I suppose the protocol is
essentially the same for all of them.

Thanks a lot.

Rubén López Santiago
Head. Laboratory of Cellular Immunology.
E.N.C.B.-I.P.N.
Apdo. Postal M-1551
Palacio Postal.
06002 Mexico, D.F. MEXICO

Phone: (525) 729-6300 ext. 62368
Fax: (525) 729-6300 ext. 62489
e-mail: rlopez@bios.encb.ipn.mx
           rsantiag@redipn.ipn.mx


---




From owner-immuno@hgmp.mrc.ac.uk  Fri Jun 16 19:06:14 2000
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From: larrymysz@aol.com (LarryMysz)
X-Newsgroups: bionet.immunology
Subject: Re: ELISA Plate Reader Software
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In article <393FB4BF.13FF39B4@genitope.com>,
  naomi@genitope.com wrote:
> I'm currently using Molecular Device's SoftmaxPro ELISA plate reader
> software to read ELISA plates and was wondering if I could get
> comments/suggestions/recommendations on other ELISA plate reader
> software?
>
> -Martin
>

Maybe I can help.

My company has developed ELISA reader software which is installed in about 200
labs.  We have a new version that simply collects absorbance data from a plate
and places it in an Excel workbook.

Prior versions of the program communicate with Molecular Devices readers, but
this latest version has not yet been programmed for them.  The lab where I
previously tested Molecular Devices equipment now has a different reader.

I'll provide a free copy of the software in exchange for a brief telephone
conversation and a little test time on a Molecular Devices reader.

You can see a program description and download a sample copy of ELISACalc Lite
from:
http://customized-applications.com

For more details, please contact me at my corporate e-mail address
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From owner-immuno@hgmp.mrc.ac.uk  Fri Jun 16 19:33:20 2000
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From: Wenwu Jin <wxj8@po.cwru.edu>
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Subject: Help: Angiotensin II receptor AT1 antibody
Date: Fri, 16 Jun 2000 14:37:56 -0400
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We have been trying to set up Western Blot analysis for angiotensin II
AT1 receptor in mouse kidney.  We have tried the antibody from Santa
Cruz Biotechnology (cat# sc-1173) without success.  I wonder if anyone
could provide any advice and/or information about sources of antibodies
for AT1 receptors.
Any help will be greatly appreciated.
Thank you.

Wenwu Jin




From owner-immuno@hgmp.mrc.ac.uk  Sat Jun 17 14:52:38 2000
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The article "My Fight Against Multiple Sclerosis" by Roger MacDougall which 
can be
found at www.cadvision.com/embrya/roger.html is very interesting.  He 
recovered by
following a diet which eliminated foods causing allergic reactions.  Some 
additional
reading on the internet indicates that small particles of undigested foods 
may enter
the bloodstream and cause allergic reactions.  This made me wonder if it 
might be
better to try to prevent absorption of undigested food particles rather than 
trying to
avoid eating foods which will cause problems.  Insoluble fiber and adequate 
liquid
will probably keep the particles larger and less likely to be absorbed.  Also 
using
means to maintain or improve the integrity of the lining of the intestines 
might help.
Glutamine supplements are supposed to do this by providing protein for the 
lining.
Ralph L. Samson


---




From owner-immuno@hgmp.mrc.ac.uk  Sun Jun 18 11:19:03 2000
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From: Genetic Engineer <netman@glide.net.in>
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--------------B48D50B8C0FB2F3C8F97BD5B
Content-Type: text/plain; charset=us-ascii
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    Hello,

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Netman

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--------------B48D50B8C0FB2F3C8F97BD5B
Content-Type: text/html; charset=us-ascii
Content-Transfer-Encoding: 7bit

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--------------B48D50B8C0FB2F3C8F97BD5B--





From owner-immuno@hgmp.mrc.ac.uk  Mon Jun 19 06:31:16 2000
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From: Ian Davis <Ian.Davis@ludwig.edu.au>
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Subject: "Th1-type" antibodies
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Dear All,

<entering sweeping generalisation mode>
In the mouse, IgG1 antibody responses tend to be induced in the context
of a Th2-type cytokine microenviroment and IgG2a for Th1-type
cytokines.  In the human, IgG4 is probably the counterpart to IgG1 in
the mouse.  What is the human counterpart for the Th1-type antibody?

Email replies preferred please to Ian.Davis@ludwig.edu.au

Thanks,
Ian Davis.




From owner-immuno@hgmp.mrc.ac.uk  Mon Jun 19 14:11:05 2000
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From: Mike Clark <mrc7@cam.ac.uk>
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Subject: Re: "Th1-type" antibodies
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In article <394DB026.246D@ludwig.edu.au>, Ian Davis
<URL:mailto:Ian.Davis@ludwig.edu.au> wrote:
> Dear All,
> 
> <entering sweeping generalisation mode>
> In the mouse, IgG1 antibody responses tend to be induced in the context
> of a Th2-type cytokine microenviroment and IgG2a for Th1-type
> cytokines.  In the human, IgG4 is probably the counterpart to IgG1 in
> the mouse.  What is the human counterpart for the Th1-type antibody?
> 
> Email replies preferred please to Ian.Davis@ludwig.edu.au
> 
> Thanks,
> Ian Davis.
> 

I would be inclined to be cautious over drawing parallels between mouse and
human IgG subclasses. My reasoning is quite simple. It is clear from an
analysis of the gene organisation and the gene and protein sequences that
the subclasses of IgG in mouse and human have arisen independently. 

The numbers of IgG subclasses vary from species to species so there is
really no valid scientific argument to assume parallels between
IgG subclasses for distantly related mammalian species such as rodents and
primates.

Mike                             <URL:http://www.path.cam.ac.uk/~mrc7/>
-- 
Mike Clark, PhD. Part II Pathology Organiser
Cambridge University, Dept. Pathology
Tennis Court Rd., Cambridge CB2 1QP
Tel.+44 1223 333705  Fax.+44 1223 333875




From owner-immuno@hgmp.mrc.ac.uk  Mon Jun 19 18:21:43 2000
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From: gloriacotter@aol.com (GloriaCotter)
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Subject: New "Health Only" search engine-contributions welcome
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From owner-immuno@hgmp.mrc.ac.uk  Mon Jun 19 22:39:29 2000
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From: Ian Davis <Ian.Davis@ludwig.edu.au>
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Subject: Re: "Th1-type" antibodies
Date: Tue, 20 Jun 2000 07:39:32 +1000
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Mike Clark wrote:
> I would be inclined to be cautious over drawing parallels between mouse and
> human IgG subclasses. My reasoning is quite simple. It is clear from an
> analysis of the gene organisation and the gene and protein sequences that
> the subclasses of IgG in mouse and human have arisen independently.
> 
> The numbers of IgG subclasses vary from species to species so there is
> really no valid scientific argument to assume parallels between
> IgG subclasses for distantly related mammalian species such as rodents and
> primates.

Dear Mike,

Thanks for your comments.  Your point is taken and of course is quite
correct, however perhaps I should phrase my question differently: is
there a human IgG subclass that is preferentially induced in the context
of a Th1-type microenvironment?  Such a class exists in the mouse.

Thanks,
Ian.




From owner-immuno@hgmp.mrc.ac.uk  Tue Jun 20 04:16:52 2000
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Subject: Re: IgA Deficiency: Any Treatments ??
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Marc-

I am a 44 year old male diagnosed with IgA deficiency about 3
years ago.  For me the symptoms are continuous colds from
October through May-1 every 2-3 weeks.  My internist is a gastro
specialist who sits on various FDA committees.  In addition he
has degrees in pharmacology and nutrition.  He mentioned that
some of the leading edge research on crohn's disease suggests
that using lactobacillus can create IgA's in the intestinal
tract.  In January I started taking Lactobacillus GG 2x a day
and have not had a cold since mid February.  On occassion, when
I have felt a cold coming on(about 3 or 4 times)I have used a
product called Zicam which is a zinc nasal spray.  The
combination seems to be very effective for me.  I have 3 kids
and that I have managed to avoid illness is a unique
experience.  Beyond that, the only things I do is  minimize
sugar and work out regularly.  Hope this helps.

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From owner-immuno@hgmp.mrc.ac.uk  Tue Jun 20 14:41:04 2000
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The account I read of Roger MacDougall raises several problems:

He did not achieve recovery after 4 years of being on his diet. Not
evidence.
His diagnosis may have been suspect, I suspect he had a viral infection that
caused his problems.
Roger himself stated that many others followed his diet without success. At
first he thought that they mustn't have followed it closely enough but upon
closer inspection dismissed this idea.
If MS could be caused by his solution then given the numbers of people that
have tried it a cure would be now be obvious ... no conspiracies theories
please.
If MS was simply an allergic reaction (though the implication of TNFa and
mast cell involvement leans towards this) surely this would have been
established by now via familial studies.
Our bodies are always fighting off foreign particles, lining the gut wall
will solve nothing and may lead to nutrient deficiencies.

I had a brief look at this months ago because some blunderheads told me that
the cure for MS was obvious (they heard of Roger's account too) but of
course the medical profession would not recognise this because they are in
cohorts with the drug companies. I'm not saying you're saying this but would
like to point out that Roger himself was much more circumspect of his
methods than many of those who picked up on his idea. I think there is merit
in exploring the allergen idea, but only for heuristic purposes. The answer
is going to be, already is, much more complex than that.

Personally, my non professional advice to people with MS is:

avoid strenuous exercise, this increase ROS and raises heat shock protein
levels, which may be implicated also ...
Avoid stress, raises pkc activity which probably exacerbates the condition
via inflammatory pathways.
Sleep well, may help lower il1 levels.

And for science fiction:

LOWER your immune response potential (ginkgo biloba may help here but
doubtful) as this may slow the disease but there are complications with
that. I don't know, it's damn difficult.


John H.
Remove 4x

<RSAMSON18@cs.com> wrote in message news:14.50fbebe.267cdc9a@cs.com...
> The article "My Fight Against Multiple Sclerosis" by Roger MacDougall
which
> can be
> found at www.cadvision.com/embrya/roger.html is very interesting.  He
> recovered by
> following a diet which eliminated foods causing allergic reactions.  Some
> additional
> reading on the internet indicates that small particles of undigested foods
> may enter
> the bloodstream and cause allergic reactions.  This made me wonder if it
> might be
> better to try to prevent absorption of undigested food particles rather
than
> trying to
> avoid eating foods which will cause problems.  Insoluble fiber and
adequate
> liquid
> will probably keep the particles larger and less likely to be absorbed.
Also
> using
> means to maintain or improve the integrity of the lining of the intestines
> might help.
> Glutamine supplements are supposed to do this by providing protein for the
> lining.
> Ralph L. Samson
>
>
> ---





From owner-immuno@hgmp.mrc.ac.uk  Thu Jun 22 06:50:08 2000
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From: Marc <mns@onepine.com>
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Subject: Re: IgA Deficiency: Any Treatments ??
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Redward,
                        Thanks for your response. I have an appointment
to see an allergist next
month, a recommendation from another physician. I will take you up on
you suggestion concerning lactobacillus and let you know. Greatly
appreciate your response to my post, glad that you found something that
apparently works. I went through an entire battery of test shots a few
years back (pneumonia, tetanus, etc, etc....) only to be told that
nothing could be done (Mt.Sinai immunology dept. in New York). Will keep
informed of any progress and/or prescriptions that I come up with.
Thanks again,

Marc

redward wrote:

> Marc-
>
> I am a 44 year old male diagnosed with IgA deficiency about 3
> years ago.  For me the symptoms are continuous colds from
> October through May-1 every 2-3 weeks.  My internist is a gastro
> specialist who sits on various FDA committees.  In addition he
> has degrees in pharmacology and nutrition.  He mentioned that
> some of the leading edge research on crohn's disease suggests
> that using lactobacillus can create IgA's in the intestinal
> tract.  In January I started taking Lactobacillus GG 2x a day
> and have not had a cold since mid February.  On occassion, when
> I have felt a cold coming on(about 3 or 4 times)I have used a
> product called Zicam which is a zinc nasal spray.  The
> combination seems to be very effective for me.  I have 3 kids
> and that I have managed to avoid illness is a unique
> experience.  Beyond that, the only things I do is  minimize
> sugar and work out regularly.  Hope this helps.
>
> Got questions?  Get answers over the phone at Keen.com.
> Up to 100 minutes free!
> http://www.keen.com




From owner-immuno@hgmp.mrc.ac.uk  Thu Jun 22 15:45:57 2000
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From: kevin-ault@uiowa.edu (Kevin Ault)
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I am aware of one study in my field of interest that is relevant to
this thread.  de Gruijl and colleagues published a paper about HPV
antigens and precancerous lesions of the uterine cervix.  It was in
the International Journal of Cancer in 1996.  Basically they were able
to correlate an IgG2 response to clearance of the viral infection and
they concluded that this indicated IFN gamma production at the site of
infection.   -  Kevin Ault MD University of Iowa USA
(kevin-ault@uiowa.edu) 




From owner-immuno@hgmp.mrc.ac.uk  Thu Jun 22 19:19:51 2000
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MedCell Biologics, Inc (www.medcellbio.com) is a private
biopharmaceutical company located in San Diego with the following job
openings:

Production Scientist/Biomanufacturing- Ph.D. in bioengineering,
immunology or other relevant field or BS or MS with 3 or more years
experience in large-scale culture of human cells.  Knowledge and
experience with large-scale mammalian cell culture, large-scale immune
cell subset purification techniques, cGMP manufacturing of biological
products, process monitoring of oxygen, pH, glucose, lactate and
ammonia, endotoxin assay and sterility testing.  Experience with
preparation of LAK cells, TIL cells, dendritic cells, stem cells or
cells for bone marrow transplantation for infusion is highly desired.   

Immunologist/QA-QC- Ph.D. in Immunology or equivalent field or BS or MS
with 3 or more years experience with immunological techniques. 
Experience in flow cytometry analysis of cell viability, DNA analysis
and intracellular cytokine expression,  RNA protectionase assay, RT-PCR,
cytokine ELISA, NK function assay, measurement of T-proliferative
responses, immunomagnetic purification of T-cell subpopulations, 
measurement of cytotoxic T-cell activity and quantitation of functional
T-cells by limiting dilution is desired.  Familiarity with cGMP for
biologicals would be helpful. 

Research Scientist/Technician:  BS/MS Immunology or related discipline
with over 2 years experience in immunological techniques.  Experience in
isolating and characterizing human lymphocytes, tissue culture (e.g.,
establishing and maintaining cultures of primary  human cells), flow
cytometry and general immunological methodology (e.g., cytokine ELISAs,
internal cytokine staining and cell proliferation assays). 

Research Scientist/Technician: BS/MS with 3 years experience in
molecular biology and immunology. The candidate will be highly familiar
with the FACS staining and analysis of cell surface markers, and
cell-based assays related to immunology or inflammation. The candidate
will be skilled in working with antibodies and most aspects of molecular
biology including RNA isolation, PCR, and northern and western blot
analyses. The applicant must have excellent verbal and written
communication skills, be highly organized, and capable of handling
concurrent projects. Preference will be given to candidates with
experience in immunology, inflammation or a related field.

Interested applicants should send a cover letter, CV and salary history
to:

Dr Micheal Gruenberg
mgruenberg@medcellbio.com




From owner-immuno@hgmp.mrc.ac.uk  Sat Jun 24 22:21:39 2000
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From: RSAMSON18@cs.com
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The book "MS Something Can Be Done and You Can Do It" by Robert W. Soll, M.D.,
Ph.D., favors The Composite Theory which includes both The Viral Theory and 
the
Autoimmune Theory.  He includes food allergies in the autoimmune part.  One
interesting fact is that MS is more prevalent among women.  One study said 
women
were 75% of the affected and another statistic said they were 60%.  This 
suggests
that the reproductive capability might be involved.  There might be an 
autoimmune
response to the foetus and/or there might be a response to the semen and 
sperm from the male.  The age pattern also favors this.
Ralph L. Samson


---




From owner-immuno@hgmp.mrc.ac.uk  Sun Jun 25 10:40:12 2000
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From: Jamie Cunliffe <cunlij@my-deja.com>
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In article <c4.5799635.26868055@cs.com>,
  RSAMSON18@cs.com wrote:
> The book "MS Something Can Be Done and You Can Do It" by Robert W.
Soll, M.D.,
> Ph.D., favors The Composite Theory which includes both The Viral
Theory and
> the
> Autoimmune Theory.  He includes food allergies in the autoimmune
part.  One
> interesting fact is that MS is more prevalent among women.  One study
said
> women
> were 75% of the affected and another statistic said they were 60%.
This
> suggests
> that the reproductive capability might be involved.  There might be
an
> autoimmune
> response to the foetus and/or there might be a response to the semen
and
> sperm from the male.  The age pattern also favors this.
> Ralph L. Samson
>
> ---
>

You may be interested to look at my web site (below) and the article on
Behcet's syndrome. This has a lot to say about MS. It shows how the
disease is likely to be a combination of auto-immune reactivity (auto-
rejection), response to hidden infection and some elements of
vasculitis BUT, all of these are manifestions of the immune rejection.
It also has a lot to say on why women have a higher incidence of MS but
men get a more severe form of the illness. It shows how a variety of
factors affect it and it shows that, along with many other "auto-
rejective" illnesses it is modulated by menstruation, pregnancy, stress
and aging (the Clinical Morphostasis article has more to say on this).

Jamie

Waterside Health Centre, SO45 5WX, UK
Home pages
http://www.ndirect.co.uk/~greenprac/jamie.html


Sent via Deja.com http://www.deja.com/
Before you buy.




From owner-immuno@hgmp.mrc.ac.uk  Mon Jun 26 02:51:50 2000
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From: jaymone@paonline.com ("Jay Mone")
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Subject: Re: IgA Deficiency: Any Treatments
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Marc,
I read with interest your post about IgA deficiency, and the suggestion of
lactobacillus as a treatment.  As a microbiologist, I have never seen
anything suggesting that lactobacillis has any therapeutic effect on this
disorder, which, presumably, has a genetic basis.  As you probably know,
antibodies (of which IgA is one type), are very specific for the substances
which induce their production.  A cold virus induces Abs against that
particular virus, and no other.  With these two basic ideas (genetics and
specificity) on the table, I fail to see how lactobacillis would induce Abs
against anything other than lactobacillis.  It is very likely, although I'm
not that familiar with this area of the literature, that lactobacillis
induces IgA Abs (in people who can produce them) since the bacteria inhabits
the intestinal surfaces (among other places) where IgA is the most important
Ab type.  How this would help you prevent colds and other respiratory
infections is beyond me.  If you have some insight, I would be interested in
learning about it.

Jay Mone'


---




From owner-immuno@hgmp.mrc.ac.uk  Mon Jun 26 11:37:17 2000
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From: Mike Clark <mrc7@cam.ac.uk>
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In article <200006252151343.SM00303@[216.220.164.55]>, Jay Mone
<URL:mailto:jaymone@paonline.com> wrote:
> Marc,
> I read with interest your post about IgA deficiency, and the suggestion
> of lactobacillus as a treatment.  As a microbiologist, I have never seen
> anything suggesting that lactobacillis has any therapeutic effect on this
> disorder, which, presumably, has a genetic basis.  As you probably know,
> antibodies (of which IgA is one type), are very specific for the
> substances which induce their production.  A cold virus induces Abs
> against that particular virus, and no other.  With these two basic ideas
> (genetics and specificity) on the table, I fail to see how lactobacillis
> would induce Abs against anything other than lactobacillis.  It is very
> likely, although I'm not that familiar with this area of the literature,
> that lactobacillis induces IgA Abs (in people who can produce them) since
> the bacteria inhabits the intestinal surfaces (among other places) where
> IgA is the most important Ab type.  How this would help you prevent colds
> and other respiratory infections is beyond me.  If you have some insight,
> I would be interested in learning about it.
> 
> Jay Mone'
> 

I would agree with the above. One of the most common causes of IgA
deficiency is a genetic defect in the ability to make IgA (commonly there
is a gene deletion). For this type of IgA deficiency there no chance of
stimmulating IgA production through any form of antigen challenge.

Mike Clark,                        <URL:http://www.path.cam.ac.uk/~mrc7/>
-- 
 o/ \\    //            ||  ,_ o   M.R. Clark, PhD. Division of Immunology
<\__,\\  //   __o       || /  /\,  Cambridge University, Dept. Pathology
 ">    ||   _`\<,_    //  \\ \> |  Tennis Court Rd., Cambridge CB2 1QP
  `    ||  (_)/ (_)  //    \\ \_   Tel.+44 1223 333705  Fax.+44 1223 333875




From owner-immuno@hgmp.mrc.ac.uk  Mon Jun 26 16:45:08 2000
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Hi there is another quirky idea regarding some autoimmune diseases which
suggests that maternal-fetal traffic of immature or virgin T cells may
contribute to disease progression.  Traffic can go both ways but may, for
women, be more important as a pregnant person as opposed to being the fetus.
Diana Bianchi, who work with the herzenbergs back in the day, has found male
cells in a women who's last pregnancy (her son) was 27 years prior.  Kinda
interesting don't you know!
markH





From owner-immuno@hgmp.mrc.ac.uk  Tue Jun 27 03:45:09 2000
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From: "Peter Jaskolski" <jaskolski@optusnet.com.au>
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Subject: IGF-IIR
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I have been searching with little success for an antibody against the
(murine or human) IGF-II receptor. if anyone knows of a company or research
group likely to have this antibody, please reply. Thank you.
Ruth jaskolski





From owner-immuno@hgmp.mrc.ac.uk  Tue Jun 27 16:20:17 2000
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"Bacteria Pharg"  - Info Please

I am looking for any leads (sites, links, snail mail addresses, books,
people) related to the subject and research of "Bacteria Pharg " (may not be
spelt correctly)

My Daughter has just finished a Biology  Degree and was very interested in a
program on TV about 2 years ago on  UK TV (don't know the channel) but all
enquires drawn a blank can anyone help.

Thank you.

P Charlton







From owner-immuno@hgmp.mrc.ac.uk  Tue Jun 27 16:22:07 2000
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From owner-immuno@hgmp.mrc.ac.uk  Tue Jun 27 19:27:59 2000
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From: "Joe Chandler" <jchandler@mainebiotechnology.com>
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Hi all,
We have a few fusions, particularly in rat spleen cells x mouse myeloma,
where there is an preponderance of fibroblasts growing int he fusion plate.
There does not appear to be a relationship between the immungen and the
occurance of the fibroblasts (but I haven't thoroughly analyzed this
possibility).  Very often the fibroblasts starve the burgeoning hybridomas
and we losse them.  I vaguely remember a kind of plate or some procedure
that thwarted fibroblast growth.  My recollection what that you used these
plates or followed this procedure when you were trying to grow primary
endothelial cells as fibroblasts were a common nuisance.  Any ideas will be
greatly appreciated.
Joe





From owner-immuno@hgmp.mrc.ac.uk  Tue Jun 27 22:50:41 2000
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From: Stacey Wilder <wilderest@msu.edu>
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Hi,
I am looking for manufacturers/researchers who can provide me with Pig
IL-5,IL-4,IL-10 and TGF-b Cytokines and antibodies.Any info would be of
great help.
Thanks in advance.
Geetha
mail to :parthasa@pilot.msu.edu




From owner-immuno@hgmp.mrc.ac.uk  Wed Jun 28 08:40:09 2000
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From: Tarmo Humppi <tarmo.humppi@pvtt.mil.fi>
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Subject: Aggregation of IgG during purification
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We have tried to purify polyclonal rabbit antibodies from serum using
affinity column that has the peptide antigen attached to it. The
problem is that the eluted IgG aggregates immediately after elution. We
have used Tris buffer pH 7.5 as starting buffer and glysine pH 3.0 for
elution. Is there a way to get the aggregated IgG back to solution? We
have tried increasing ionic strenght but it hasn´t helped. All tips are
appreciated!

--
Tarmo


Sent via Deja.com http://www.deja.com/
Before you buy.




From owner-immuno@hgmp.mrc.ac.uk  Wed Jun 28 10:25:10 2000
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From: Mike Clark <mrc7@cam.ac.uk>
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In article <8jc99o$pfg$1@nnrp1.deja.com>, Tarmo Humppi
<URL:mailto:tarmo.humppi@pvtt.mil.fi> wrote:
> We have tried to purify polyclonal rabbit antibodies from serum using
> affinity column that has the peptide antigen attached to it. The
> problem is that the eluted IgG aggregates immediately after elution. We
> have used Tris buffer pH 7.5 as starting buffer and glysine pH 3.0 for
> elution. Is there a way to get the aggregated IgG back to solution? We
> have tried increasing ionic strenght but it hasn´t helped. All tips are
> appreciated!
> 

The 'aggregated immunoglobulin' is quite likely to include denatured
protein which is unlikely to completely renature. Firstly do you you need
to get it all back into solution? Have you estimated the activity of the
starting and the eluted material (that is the total activity e.g. titre x
volume not the specific activity e.g. titre) ? Perhaps you have recovered
enough anyway. There will always be some losses in any purification
process. It may also be that you have eluted at too low a pH to start with.
The way low pH works is that you are partially dentaturing the antibody Fab
to elute it. As your antigen is a peptide I would be surprised if the
affinity is so high to require pH3. Try a stepwise elution using pH3.5,
pH3.2, pH3.0 and perhaps finish with pH2.5 to clean up the column. Check
the activity in each eluate after dialysis and pool the appropriate
fractions.


Mike Clark,                        <URL:http://www.path.cam.ac.uk/~mrc7/>
-- 
M.R. Clark, PhD. Division of Immunology
Cambridge University, Dept. Pathology
Tennis Court Rd., Cambridge CB2 1QP
Tel.+44 1223 333705  Fax.+44 1223 333875




From owner-immuno@hgmp.mrc.ac.uk  Wed Jun 28 11:08:07 2000
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From: "M. Corradi" <lab2NOSPAM@plantechno.com>
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Subject: help for phage-display
Date: Wed, 28 Jun 2000 12:03:17 +0200
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Hi all!
There is in this newsgroup some researcher from Italy who use phage-display
for selection of antibodies? I have to work with it but I'm not so
experienced and I encountered some problems, probably because following a
protocol is not always enough.
I would like to discuss with someone expert and, if it's possible for him,
I'll come with pleasure to see an application.
Thanks in advance

Dr. Corradi Massimiliano






From owner-immuno@hgmp.mrc.ac.uk  Wed Jun 28 22:10:22 2000
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From: lindow@molpharm.dk
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Subject: cDNA for mouse IgG1 HC constant regions
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Hi group

I want to make a fusion protein with the constant regions of mouse IgG1
heavy chain. If anyone can help obtaining the cDNA for that gene, I
would be extremely grateful. It doesn't seem to be in my mouse spleen
cDNA library.

Cheers,

Morten Lindow
Lab Molecular Pharmacology
Copenhagen University
Denmark
lindow@molpharm.dk


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From owner-immuno@hgmp.mrc.ac.uk  Wed Jun 28 22:11:36 2000
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Peter C <pjc-techmor@cwcom.net> wrote in message
news:YC365.2682$uO1.29797@news1-hme0...
> "Bacteria Pharg"  - Info Please
>
> I am looking for any leads (sites, links, snail mail addresses, books,
> people) related to the subject and research of "Bacteria Pharg " (may not
be
> spelt correctly)
>
> My Daughter has just finished a Biology  Degree and was very interested in
a
> program on TV about 2 years ago on  UK TV (don't know the channel) but all
> enquires drawn a blank can anyone help.
>
> Thank you.
>
> P Charlton
>
>

Bacteriophage?

 I can't believe anyone can do a biology degree and not know something about
bacteriophages - viruses th