From owner-insulin@net.bio.net Wed Apr 02 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Rob Steele Newsgroups: bionet.cellbiol.insulin Subject: lectin receptor protein-tyrosine kinase Date: 3 Apr 1997 07:45:09 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 26 Sender: daemon@net.bio.net Approved: tpillay@ucsd.edu Distribution: world Message-ID: <5i0ja5$81u@net.bio.net> NNTP-Posting-Host: net.bio.net Since this group apparently needs something to sink its teeth into, I'll provide some bait. We have discovered what I believe to be the first of its kind among receptor protein-tyrosine kinases. It is an RTK in which the extracellular portion contains four C-type lectin domains. Thus we have a RTK which potentially has carbohydrates as its ligands rather than proteins. We found the gene for this receptor while PCR'ing for genes encoding receptor protein-tyrosine kinases involved in pattern formation in the simple animal hydra. Database and literature searches have turned up no other examples of this type of RTK. Is anyone in this group aware of any other examples of RTKs which contain C-type lectin domains? ================================================================================ Rob Steele Associate Professor Department of Biological Chemistry 240 D Medical Sciences I University of California, Irvine Irvine, CA 92697-1700 office phone: 714-824-7341 lab phone: 714-824-4343 fax: 714-824-2688 e-mail: resteele@uci.edu www: http://128.200.5.5/steele.html From owner-insulin@net.bio.net Wed Apr 09 23:00:00 1997 Path: biosci!biosci!not-for-mail From: mbzrl@mbn1.biochem.nottingham.ac.uk (Rob) Newsgroups: bionet.cellbiol.insulin Subject: recombinant IAPP Date: 10 Apr 1997 12:12:23 -0700 Organization: University of Nottingham Lines: 8 Sender: daemon@net.bio.net Approved: tpillay@ucsd.edu Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hi Does anyone know whether IAPP has ever been expressed sucessfully in E. coli as, for example, a GST fusion ? Thanks Rob From owner-insulin@net.bio.net Wed Apr 09 23:00:00 1997 Path: biosci!biosci!not-for-mail From: sld95@skynet.be (Grognet Pierre) Newsgroups: bionet.cellbiol.insulin Subject: Rat insulin MoAbs Date: 10 Apr 1997 12:12:26 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Approved: tpillay@ucsd.edu Distribution: world Message-ID: <1.5.4.32.19970410110714.006c8ca4@mail.skynet.be> NNTP-Posting-Host: net.bio.net Dear colleagues, Does anyone know where I could find paired antibodies (MAbs) for the ELISA of rat insulin/proinsulin. Thanks in advance. P. Grognet IBB-SLD 2 Avenue du Barbeau B-1160 Brussels Belgium Fax ++32 (0)2.520.32.82 From owner-insulin@net.bio.net Sun Apr 13 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "Volkhard Helms" Newsgroups: bionet.cellbiol.insulin Subject: 3D structure modelling Date: 14 Apr 1997 01:38:28 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 67 Sender: daemon@net.bio.net Approved: tpillay@ucsd.edu Distribution: world Message-ID: <9704111120.ZM10944@chemcca10.ucsd.edu> NNTP-Posting-Host: net.bio.net Hi John, > I have read in the literature that it is possible to model the 3D > structure of kinase domains based on the coordinates of existing crystal > structures: considering that the catalytic core of protein kinase seems to be pretty well conserved in crystal structures of different protein kinases, homology modelling of other family members seems to be quite feasible > How useful is this technique? (ie, how accurate, esp with regard to loop > structures) there are no limitations to accuracy per se. It just depends how similar the structure of your target protein and those of possible templates are. One challenge is to find the best template(s), e.g. from protein sequence comparison, since there are now a number of protein kinase structures available. Some methods allow you to use a number of templates as input. Another question is whether you are mostly interested in a coarse picture of the overall structure of 'your' protein kinase, or in specific details such as the active site conformation. Obtaining an accurate active site model or accurate loop conformations would probably be asking too much from pure homology modelling. You might improve the model by including biochemical data in some way. > How might I proceed to do this? (access to data, programs/computer > facilities) I am not really an expert in this ... Data: you can download crystal structures from the Protein Data Bank via WWW at http://pdb.pdb.bnl.gov/ Computer facilities: best if you have access to a graphics workstation, maybe a powerful PC might do as well. Programs: * MSI has a Homology module that is quite popular and is interfaced to the INSIGHT software, probably expensive + I don't think it runs on PCs * MODELLER is an academic software written by A. Sali at Rockefeller, especially written for homology modelling, might work on PC ? * WHATIF is a super-fancy academic graphic program by G. Vriend at EMBL Heidelberg (license ca. 250 $), runs also on PC, is a bit tricky to learn, but is VERY powerful * there are certainly others, these are the once I have heard of the proper choice probably depends on how deep you want to get into this and whether you have access to INSIGHT e.g. You will be able to find many excellent examples of homology modelling in the literature, there exist also methods how you can estimate how good your model is. Check in the journals 'Proteins', 'Protein Science', and 'Protein Engineering'. give it a try! Volkhard Helms p.s. some early examples for protein kinases are Knighton et al. Science, 258, 130 and Knighton et al. PNAS, 90, 5001 but there are more powerful techniques today From owner-insulin@net.bio.net Wed Apr 23 23:00:00 1997 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.cellbiol.insulin Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 23 Apr 1997 23:25:03 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 242 Sender: daemon@net.bio.net Approved: tpillay@ucsd.edu Distribution: world Message-ID: <199704220900.CAA01331@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. 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For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-insulin@net.bio.net Wed Apr 23 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "Robert W. Georgantas III" Newsgroups: bionet.cellbiol.insulin Subject: Help with a Posting Date: 23 Apr 1997 23:25:06 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 127 Sender: daemon@net.bio.net Approved: tpillay@ucsd.edu Distribution: world Message-ID: <01IHPC8PZ6N28WVYN4@jhmail.hcf.jhu.edu> NNTP-Posting-Host: net.bio.net I am a student with the Department of Pharmacology and Molecular Sciences at Johns Hopkins University, School of Medicine. I have started up a small internet site called "The Science Guide" located at the URL http://www.scienceguide.com. I started the site to address the lack of a unifying source of information for scientists on the internet. As you can see in the announcement below the Guide consists of a number of sections designed to help scientists find information on the internet, The news section is currently our most popular service receiving over 4K visits per day, with a hundreds of subscribers to the Daily News Emailer. Since we do not charge for our service, and since we are not accepting advertisers; we have only a small number of routes by which to spread the word about the Guide (we have no advertising budget as well). One of these routes is by posting to Usenet groups. We have done this on a small basis by looking at a news group to assure that the users of the group are appropriate for our service, and then posting the Announcement enclosed below to the group. We Do Not Spam. Over the last month we have posted to roughly forty news groups and have received a great response in the form of emails telling us how useful our site is, and will become as we add more content to it. We have not received a single negative response, which we have interpreted to mean that our site is useful enough to scientists that they do not mind our "off-subject" posting. That brings me to the crux of the email, I was hoping that you could take the time to visit the Science Guide, determine if it would be of use to the subscribers of the group which you modify, and if so - post the announcement text below to the group. This would be of great help to us in getting the word out about the Guide. If you feel that this posting would be inappropriate, please accept my apologies for having taken your time, and just disregard this letter. One thing that may be particularly of interest, is our plan to start monitoring Congress for bills containing items concerning scientific funding. We plan on doing this to take advantage of the power of the internet to set up a pseudo lobbying group. As an example - during the Congressional Debates over the NIH budget a few months ago we encouraged our readers to email their congress-person asking for an increase in the budget. At the time we had just started the guide and had only a small number of readers, but now that we have literally thousands of users (and our usership is growing at 40% per week) and we think that we could make a significant difference in funding by informing our users of congresses' actions and helping them to act in writing to the House, or Senate, or President. Thank you Robert Georgantas - The Science Guide PS. You can check to make sure that I am actually who I say that I am buy fingering my student email account: rgeorgan@welchlink.welch.jhu.edu. The Science Guide is hosted under the .com domain by Hway Technologies, because Hopkins was not willing to host a site with our potential traffic. ------------------Announcement Text------------------------ Announcing the SCIENCE GUIDE. http://www.scienceguide.com A New Internet Directory and Information Service run by Scientists and Physicians for Scientists and Physicians. After visiting the Guide, If you have any suggestion for making the Guide better please let us know. (webmaster@scienceguide.com) The Science Guide consists of a number of different sections designed to help the scientist and physician find information on the internet and to sponsor communication between those interested in science: NEWS SECTION Every day the Science Guide compiles medical and research news from national news sources around the net. Most of the news articles are concerned with medicine, bioscience, and physics, but all other sciences from agriculture to zoology are commonly included. News sources currently listed include: CNN, EurekAlert, HMS Beagle, MSNBC Sci-Tech, Science Magazine's ScienceNow, CBS Space News, USA Today, The Albuquerque Journal, Scientific American Web Weekly, The Why Files, Discover Magazine, Scientific American, Smithsonian Magazine, and the Technology Review. The news pages also list links to news sources not compiled within the News site. We are currently working on adding a number of other sources to the site to make it even more useful. To make getting science news even easier, we send out a DAILY NEWS EMAILER listing the articles which have been compiled on our site. Anyone can subscribe to the Emailer by sending an email to news@scienceguide.com with the message "Subscribe" DIRECTORY OF USENET NEWS GROUPS and DISCUSSION LISTS The Directory of Usenet and Discussion Groups is compiled quarterly from different sources around the net to provide the scientist and those interested in science easy access to these invaluable sources of discourse and information. We are currently working on finding the proper subscription method for each of the discussion lists. This is taking a bit longer that we thought so please pardon our dust. The Usenet portions of this section are complete. ON-LINE JOURNAL HYPERLINK SECTION The Journals Section contains links to peer reviewed scientific journals on the Internet. Each listing clearly indicates whether the journal provides only the table of contents, TOC with abstracts, or the full text of the journal EMPLOYMENT SECTION The Jobs and Positions Section contains hyperlinks to the best Scientific Employment Databases and Classifieds on the net. GRANTS and FUNDING SECTION The funding section contains links to the best funding and grant databases on the Internet, making it very easy for scientists to quickly find funding opportunities. The featured site of the section is "The Community of Science," a Johns Hopkins service designed to help scientists find and continue funding. From owner-insulin@net.bio.net Wed Apr 23 23:00:00 1997 Path: biosci!biosci!not-for-mail From: daemon@net.bio.net Newsgroups: bionet.cellbiol.insulin Subject: IRS-2 Antibody Date: 23 Apr 1997 23:25:00 -0700 Organization: Diabetes Research Institute D=B8sseldorf Lines: 16 Approved: tpillay@ucsd.edu Distribution: world Message-ID: <3357FB79.2BD9@uni-duesseldorf.de> Reply-To: tillm@uni-duesseldorf.de NNTP-Posting-Host: net.bio.net I am searching for an IRS-2 antibody to be used for immunoprecipitation and Western blotting analysis. Is there anyone who knows where to buy an IRS-2 antibody ?! I`ve tested the IRS-2 antiserum from UBI in cardiac tissue. Martin Till Diabetes Research Institute D=FCsseldorf Auf`m Hennekamp 65 40225 D=FCsseldorf email : tillm@uni-duesseldorf.de =46ax. : 0211-3382-603 From owner-insulin@net.bio.net Mon Apr 28 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Peter Fedorcsak Newsgroups: bionet.cellbiol.insulin Subject: help in HIR IP,WB Date: 28 Apr 1997 23:37:48 -0700 Organization: Rikshospitalet Lines: 19 Sender: daemon@net.bio.net Approved: tpillay@ucsd.edu Distribution: world Message-ID: <335F34AD.BB3B1223@klinmed.uio.no> NNTP-Posting-Host: net.bio.net Hi, I've tried to immunoprecipitate the insulin receptor from HepG2 cells (5*10^6 / sample) w/ monoclonal AB from Calbiochem. On the western I detect the proteins w/ BioRad Enhanced Gold Stain (detection limit claimed to be 100 pg). I can see the heavy chains of the IgGs on the western blot, but the insulin receptor is absent. What should I do to detect them? Any suggestion appreciated. Peter Fedorcsak Rikshospitalet, Oslo From owner-insulin@net.bio.net Tue Apr 29 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "T.S. Pillay" Newsgroups: bionet.cellbiol.insulin Subject: IP from HepG2 cells Date: 29 Apr 1997 23:45:28 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Approved: tpillay@ucsd.edu Distribution: world Message-ID: <33662C47.46F9@rpms.ac.uk> NNTP-Posting-Host: net.bio.net To Peter Fedorcsak: I assume you also blotted with the same antibody: are you sure that this antibody reacts well with denatured receptor on Western blots. Perhaps your detectionantibody should be an antipeptide one or vice versa. Yours sincerely