From owner-maize@net.bio.net Thu Apr 02 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: Richard Thompson <thompson@mpiz-koeln.mpg.de>
Newsgroups: bionet.maize
Subject: anti-GUS antibodies
Date: 3 Apr 1998 06:33:15 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 18
Sender: daemon@net.bio.net
Approved: msachs@uiuc.edu
Distribution: world
Message-ID: <9804030958.AA08363@mercury.mpiz-koeln.mpg.de>
NNTP-Posting-Host: net.bio.net

Hello out there, I am looking for a supplier, commercial or otherwise, of
antibody raised to E.coli beta-glucuronidase (GUS to you!). This seems
difficult to track down, so I would be grateful for any tips.

Thanks in advance,

Richard Thompson
Richard Thompson
MPI fuer Zuechtungsforschung
Carl-von-Linn=E9 Weg 10
D-50829 KOELN, GERMANY
Tel.+49-221-5062-440
Fax.+49-221-5062-413






From owner-maize@net.bio.net Thu Apr 02 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: Ed Coe <ed@teosinte.agron.missouri.edu>
Newsgroups: bionet.maize
Subject: [Fwd: Please send me info on corn]
Date: 2 Apr 1998 20:43:10 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 32
Sender: daemon@net.bio.net
Approved: msachs@uiuc.edu
Distribution: world
Message-ID: <35245EEE.16CB7440@teosinte.agron.missouri.edu>
Reply-To: Rachel N. <AoBLover@aol.com>
NNTP-Posting-Host: net.bio.net



Received: from imo18.mx.aol.com (imo18.mx.aol.com [198.81.17.40]) by
teosinte.agron.missouri.edu (8.6.10/8.6.10) with ESMTP id VAA03722 for
<db_request@teosinte.agron.missouri.edu>; Thu, 2 Apr 1998 21:44:46 -0600
Received: from AoBLover@aol.com
	by imo18.mx.aol.com (IMOv13.ems) id NURVa14951
	for <db_request@teosinte.agron.missouri.edu>; Thu, 2 Apr 1998
22:39:11 -0500 (EST)
From: AoB Lover <AoBLover@aol.com>
Message-ID: <54f54f53.352459e2@aol.com>
Date: Thu, 2 Apr 1998 22:39:11 EST
To: db_request@teosinte.agron.missouri.edu
Mime-Version: 1.0
Subject: Please send me info on corn
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7bit
X-Mailer: AOL 3.0 16-bit for Windows sub 38

Dear whoever receives this e-mail from the Corn/Maize homepage,
        I am starting a new science project on "What is the effect of Pigments
on the growth of Corn". I'm planting  mutated albino corn seeds and regular
(pigmented) corn seeds, to find out what the effect is.
I would really appreciate it if you could send me any current information on
the growth of corn (and the growth of albino corn.)
And anything eles that you think that could contribute to my research.
Thank you very much,
Rachel N.





From owner-maize@net.bio.net Fri Apr 03 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: Ed Coe <ed@teosinte.agron.missouri.edu>
Newsgroups: bionet.maize
Subject: hulless popcorn
Date: 4 Apr 1998 07:48:42 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 24
Sender: daemon@net.bio.net
Approved: msachs@uiuc.edu
Distribution: world
Message-ID: <35264472.4BFE6B05@teosinte.agron.missouri.edu>
References: <199804040135.TAA01341@teosinte.agron.missouri.edu>
Reply-To: Susan Peoples <drp@gnat.net>, maize@net.bio.net
NNTP-Posting-Host: net.bio.net

drp@gnat.net wrote:

> message originates from 207.144.101.222 891653711
> Submittor's Name: Susan Peoples
> Submittor's Email Address: drp@gnat.net
> Comments Follow:
>
> I am in Georgia and would like to purchase some hulless popcorn due to
>orthodontic reasons.  Please notify me on where I may obtain this popcorn.



It is sometimes called Japanese Hulless.  It is my favorite popcorn,
because it is light and tasty and leaves less hulls.
I find it in my grocery as the white variety on the shelf; packages do
not always say hulless.  If not available in a regular grocery, check a
specialty grocery.
Others on the maize net may have further information.
Ed Coe






From owner-maize@net.bio.net Mon Apr 06 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: ttsonev@obzor.bio21.acad.bg
Newsgroups: bionet.maize
Subject: FESPP Congress: Deadline for abstracts is 30 April
Date: 7 Apr 1998 06:58:28 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 23
Sender: daemon@net.bio.net
Approved: msachs@uiuc.edu
Distribution: world
Message-ID: <3529F1CC.AB6FD7DE@obzor.bio21.acad.bg>
NNTP-Posting-Host: net.bio.net

Dear colleagues,

The deadline for submission of abstracts for the 11th Congress of the
Federation of European Societies of Plant Physiology (FESPP) has been
extended to 30 April 1998.

The Organizing Committee

Please visit our web site:
http://www.bio21.acad.bg/fespp98/
mirror site:
http://www.bulgaria.com/fespp98/

E-mail contacts:
Organizing committee:
fespp98@obzor.bio21.acad.bg
Congress Sectretariat - Company for International Meetings - CIM Ltd.:
CIM@EINET.BG






From owner-maize@net.bio.net Tue Apr 07 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: Ed Coe <ed@teosinte.agron.missouri.edu>
Newsgroups: bionet.maize
Subject: To Maize Colleagues
Date: 8 Apr 1998 11:57:39 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 18
Sender: daemon@net.bio.net
Approved: msachs@uiuc.edu
Distribution: world
Message-ID: <352BB1BB.C65D7D30@teosinte.agron.missouri.edu>
NNTP-Posting-Host: net.bio.net

Joyce Bork, Chair, Science Dept., Webster University, has informed me
that Ginny Harrison died in November after a valiant battle with
cancer.  The University will soon be naming the biology lab the Ginny
Harrison Biology Lab, and in their new building, which should be ready
in five to seven  years, her name will be on a new lab.

A memorial fund has been established at Webster University, to receive
gifts in Ginny's name toward new equipment in the Science Dept., at the
following address:

(For the Ginny Harrison Fund)
Webster University
Development Office
470 East Lockwood
St. Louis, MO 63119




From owner-maize@net.bio.net Wed Apr 08 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: limj03@mcrcr6.med.nyu.edu
Newsgroups: bionet.maize
Subject: copy number of the gene of interest
Date: 9 Apr 1998 12:49:50 -0700
Organization: NYU Medical Center, 550 First Ave., New York, NY 10016
Lines: 25
Sender: daemon@net.bio.net
Approved: msachs@uiuc.edu
Distribution: world
Message-ID: <01IVO3EDS67M8WVZX8@MCRCR6.MED.NYU.EDU>
Reply-To: limj03@mcrcr6.med.nyu.edu, maize@net.bio.net
NNTP-Posting-Host: net.bio.net

Dear Maize Netters,

Now I am facing a puzzle, so that I would like to get any help from you. The
puzzle is as follows:

I have recently cloned and sequenced a maize gene with sequence homology to
the Arabidopsis gene SCARECROW. In order to study, its expression pattern,
I have tried to make a gene specific probe for in situ. To test whether it
is really gene specific, I performed Southern, but the probe (310bp)
recognized two bands. The probe is made based on the sequences of the 3'end
of the gene. It contains 3'UTR and coding sequences (half and half). The
simplest explanation is that there may be two very related genes in maize
genome. But I recently noticed that maize is more or less allotetraploidy.
Does it matter? Any comment is welcome.

Thank you!

Jun Lim
e-mail: limj03@mcrcr6.med.nyu.edu
New York University
Bio Dept. Rm 1009
Washington Square East, NY10003




From owner-maize@net.bio.net Wed Apr 08 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: Mike McMullen <mcmullen@teosinte.agron.missouri.edu>
Newsgroups: bionet.maize
Subject: Re: copy number of the gene of interest
Date: 9 Apr 1998 14:30:44 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 33
Sender: daemon@net.bio.net
Approved: msachs@uiuc.edu
Distribution: world
Message-ID: <6gjem4$21t@net.bio.net>
Reply-To: maize@net.bio.netTo: limj03@mcrcr6.med.nyu.edu, maize@net.bio.net
NNTP-Posting-Host: net.bio.net

It only matters if you care which gene is actually expressed in a specific
tissue, developmental stage or condition.  Get rid of the coding region
and the 3' UTR will most probably be gene-specific.

Mike

On 9 Apr 1998 limj03@mcrcr6.med.nyu.edu wrote:

> Dear Maize Netters,
>
> Now I am facing a puzzle, so that I would like to get any help from you. The
> puzzle is as follows:
>
> I have recently cloned and sequenced a maize gene with sequence homology to
> the Arabidopsis gene SCARECROW. In order to study, its expression pattern,
> I have tried to make a gene specific probe for in situ. To test whether it
> is really gene specific, I performed Southern, but the probe (310bp)
> recognized two bands. The probe is made based on the sequences of the 3'end
> of the gene. It contains 3'UTR and coding sequences (half and half). The
> simplest explanation is that there may be two very related genes in maize
> genome. But I recently noticed that maize is more or less allotetraploidy.
> Does it matter? Any comment is welcome.
>
> Thank you!
>
> Jun Lim
> e-mail: limj03@mcrcr6.med.nyu.edu
> New York University
> Bio Dept. Rm 1009
> Washington Square East, NY10003




From owner-maize@net.bio.net Thu Apr 09 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: RBOUCHARD@ACS.WOOSTER.EDU (Robert A. Bouchard)
Newsgroups: bionet.maize
Subject: Re: copy number of the gene of interest
Date: 10 Apr 1998 08:38:56 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 19
Sender: daemon@net.bio.net
Approved: msachs@uiuc.edu
Distribution: world
Message-ID: <01IVPAV0AMDK8WYDT3@ACS.WOOSTER.EDU>
Reply-To: maize@net.bio.net
NNTP-Posting-Host: net.bio.net


In my experience (specifically with maize the gene families for the
ubiquitins, shsps, and hsp 80s) a probe confined *strictly* to the 3'UTR
will generally be gene-specific at normal stringencies even when the ORFs
of the related genes have very high homology.  I suggest you use pcr to
lift out the 3' UTR starting at a point just beyond the stop codon of your
coding sequence.



---

Robert A. Bouchard                        Phone:  330-263-2433 [Note: new]
Department of Biology                     FAX:    330-263-2378 [area code]
Mateer Hall                               eMail:  RBOUCHARD@ACS.wooster.edu
931 College Street,  The College of Wooster,  Wooster, Ohio 44691-2363




From owner-maize@net.bio.net Mon Apr 13 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: Matt Evans <mmevans@facstaff.wisc.edu>
Newsgroups: bionet.maize
Subject: Ds alleles of color genes in W23 or W64A
Date: 13 Apr 1998 18:53:17 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 26
Sender: daemon@net.bio.net
Approved: msachs@uiuc.edu
Distribution: world
Message-ID: <v03007800b1586a1f6610@[144.92.63.133]>
NNTP-Posting-Host: net.bio.net

I am using one of transposed Ac stocks generated by Hugo Dooner to try to
tag a gene.  However, I need to converge the Ac into a particular inbred
background to be able to see the phenotype of interest.  I was wondering if
anyone had seeds (and could share) of either bz1-m2(DI)::Ds or r-m3::Ds in
either a W23 or W64A inbred background (M14 or A158 could also work but
would not be ideal).  Alternatively,  I could use a stable bz1 allele in
one of these inbreds if available and converge the responding bz1-m2::Ds
allele along with the Ac from the stock from the coop.
Thanks,
Matt





Matthew Evans
Laboratory of Genetics
445 Henry Mall
University of Wisconsin-Madison
Madison, Wisconsin  53706
(608) 262-3286 (phone)
(608) 262-2976 (fax)





From owner-maize@net.bio.net Mon Apr 13 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: Rishi Shukla <rshukla@ews.uiuc.edu>
Newsgroups: bionet.maize
Subject: Starch Content.
Date: 14 Apr 1998 09:11:54 -0700
Organization: University of Illinois at Urbana-Champaign
Lines: 13
Sender: daemon@net.bio.net
Approved: msachs@uiuc.edu
Distribution: world
Message-ID: <Pine.HPP.3.96.980412143716.11292A-100000@glhpx1.ews.uiuc.edu>
Reply-To: Rishi Shukla <rshukla@ews.uiuc.edu>, maize@net.bio.net
NNTP-Posting-Host: net.bio.net

Hello Everyone,

              I am trying to analyze starch content of a suspension which
has under <0.2% starch. The standard procedure (amylase+glucoamylase) is
not yielding accurate/reproducible results. Could someone suggest an
alternative trick or technique.

Any pointers will be highly appreciated.

Thank you.
Rishi Shukla.



From owner-maize@net.bio.net Mon Apr 13 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: BIOSCI Administrator <biohelp@net.bio.net>
Newsgroups: bionet.maize
Subject: BIOSCI/bionet miniFAQ & Fundraiser
Date: 14 Apr 1998 06:38:36 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 235
Sender: daemon@net.bio.net
Approved: msachs@uiuc.edu
Distribution: world
Message-ID: <199804140900.CAA29880@net.bio.net>
NNTP-Posting-Host: net.bio.net

(LAST REVISION: 30-JUL-95)

This BIOSCI "miniFAQ" is designed to answer the questions that come up
the *most frequently*.  The main BIOSCI FAQ (Frequently Asked
Questions) is accessible on the World Wide Web at URL
http://www.bio.net/.

If you can not find an answer to your question in this or other
documentation, the BIOSCI technical support staff answers e-mail
queries sent to

		       biosci-help@net.bio.net

We can only answer questions about the use of the newsgroups and
mailing lists.  We unfortunately do not have the staff to do Internet
information searches or answer scientific questions.  Please post
those to the appropriate BIOSCI/bionet newsgroups.


	Contents:
	--------
	0) BIOSCI NEEDS YOUR SUPPORT!!

	1) Using the WWW to access the BIOSCI/bionet newsgroups.

	2) What to do about "spams," i.e., junk mail, ads, etc.

	3) Examples of subscribing and unsubscribing to the mailing lists.

	4) The BIOSCI user address and research interest directory.


0) BIOSCI NEEDS YOUR SUPPORT!!
------------------------------
BIOSCI's government funding has been expended, and we are now
operating solely from advertising revenue that we have raised from our
Web site at http://www.bio.net/.  We need just a few minutes of your
time to help us serve you.

You can do two important things which will take very little time for
you individually and will immensely help us continue to help you.

First, please use our WWW system at http://www.bio.net/ to access the
archives.  You can post or reply to messages via your Web browser as
described in item #1 below.  Your usage helps attract sponsors. If you
contact any of our sponsors, please be sure to thank them for
supporting BIOSCI. It is critical for them to get this feedback if
they are to continue their sponsorship for the long term.

Second, if you work for a company or organization that provides
products or services of interest to the biology community, please pass
this message on to your marketing or marketing communications
department or other appropriate group.  Please ask them to help
support BIOSCI by sponsoring our Web site and explain the uses and
benefits of the system to the biology community. If they are
interested, they can then contact us for further information at our
tech support address, biosci-help@net.bio.net.


1) Using the WWW to access the BIOSCI/bionet newsgroups.
--------------------------------------------------------
As of 10 December 1995, all BIOSCI/bionet full newsgroups are
accessible through the World Wide Web (WWW) at URL http://www.bio.net.
One can read and reply publicly or privately to both recent postings
and archived messages through one's Web browser if it is configured
properly to send e-mail.  Each newsgroup is equipped with its own WAIS
index.  The main BIOSCI home page also has access to the BIO-JOURNALS
Table of Contents database WAIS index and the BIOSCI user address
database described in another item further below.


2) What to do about "spams," i.e., junk mail, ads, etc.
-------------------------------------------------------
BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups),
mailing lists, and a hypermail archive at URL http://www.bio.net/.
The same postings are distributed on all media (except for a small
number of mailing-list-only groups at net.bio.net).  Unfortunately it
is becoming a despicable practice on the Internet (by a few people out
to make a fast buck) to do automated mass postings to thousands of
newsgroups and mailing lists.  These attempts to grab free advertising
are refered to as "spams" in the usual, somewhat boneheaded, net
terminology.  USENET is more susceptible to this practice, and many
spams originate on the USENET groups and then are passed on to the
mailing lists.  However, spammers also get lists of mailing addresses
and hit these too, so neither medium is immune.

What should you do personally if you get junk mail?
---------------------------------------------------
Just delete it and move on without reading it further.  Filing a
protest is becoming increasingly useless because spammers are often
disguising the addresses where the messages are sent from.  Unless you
really understand Internet mail systems, your attempt at protest by
sending replies to the message will often end up being sent to the
address of an innocent person that the spammer is victimizing.

What can BIOSCI/bionet do to protect its newsgroups?
----------------------------------------------------
The only solution currently available is to moderate the newsgroup.
If this newsgroup is already moderated, then you are in good shape.
Moderation protects the USENET distribution from about 95% of the
spams that are being sent to date and protects the mailing lists
completely.  Moderation means, however, that someone has to take the
time to review each message before it goes out.  We have set up
software here that simply allows the moderator to forward to an
address at net.bio.net messages that (s)he wishes to have distributed.
This takes no more time than that needed to read the message and pass
it on, say about 1 min. per message.

Most newsgroups currently have a discussion leader who is responsible
for their newsgroup.  The discussions leaders and their e-mail
addresses are listed in the BIOSCI Information Sheet which is
available on the Web at http://www.bio.net/.  If a newsgroup is being
hit with too many junk postings, please contact the discussion leader
for that group and see if there is interest in moderating the group.
Please do not assume that by simply posting a complaint to the
newsgroup itself, anyone on the BIOSCI staff will act on your
complaint.  With close to 100 newsgroups to run, the BIOSCI staff has
to rely on the discussion leaders of each newsgroup to report problems
directly to us at biosci-help@net.bio.net.

We will moderate any of our newsgroups if the discussion leader tells
us that the readership of the group wishes to do so and if a moderator
is willing to do the work.  For most BIOSCI/bionet groups, this
entails only a few minutes of work each day.

Moderating a newsgroup will resolve probably 95% of the junk postings
on the USENET distribution.  Unfortunately there are easy ways for
determined spammers to override the moderation mechanism on USENET,
but we can protect our e-mail subscribers from unwanted postings if
the newsgroup is moderated.  You can also access our newsgroups over
the WWW at URL http://www.bio.net.  While this Web interface will not
stop spammers from trying to post to the groups, this will give you
yet another way, besides using USENET news, to keep the junk out of
your personal mail files.  For those of you with local USENET news
systems, the Web interface will also give you faster access to new
newsgroups and recent postings.


3) Examples of subscribing and unsubscribing to the mailing lists.
------------------------------------------------------------------
PLEASE NOTE: The BIOSCI management does NOT act on
subscription/unsubscription requests that are posted improperly to the
newsgroups and mailing lists.  People who do this only bother everyone
on the lists to no avail.  Please be sure to follow the proper
procedures below.

Gory details are in the BIOSCI Information sheets on the Web at
http://www.bio.net.  Below we give an example utilizing the
METHODS-AND-REAGENTS list at both of our two BIOSCI sites:

Users in the Americas and Pacific Rim countries who use the BIOSCI
------------------------------------------------------------------
node at computer net.bio.net:
----------------------------

A) Determine the "listname" which is the <=8 character mail address
                                         ^^^^^^^^^^^^^
   for the group.  These can be found in the BIOSCI Info. Sheet.  For
   the METHODS-AND-REAGENTS group the mailing address is
   methods@net.bio.net.  The listname is the portion of the address to
   the left of the @ sign, i.e., "methods".  The listname is used with
   the "subscribe" and "unsubscribe" commands illustrated below.

B) Mail all commands in the body of a mail message addressed to
   biosci-server@net.bio.net.  Do NOT send commands to the newsgroup
   posting addresses!  Leave the Subject: line blank, any text on it
   will be ignored.

C) In the body of your message put one or more of the following
   commands with an "end" command on the last line, e.g.,

   subscribe methods
   unsubscribe methods
   end

   Do NOT put your e-mail address or other text on these lines.  The
   server only allows you to cancel your subscription if the address
   on your mail header matches the address on our mailing list.
   Please ask for help at biosci-help@net.bio.net if your address has
   changed, e.g., if you know you are on the list but the server tells
   you that you are not a member.


Users in Europe, Africa, and Central Asia who use the BIOSCI node at
--------------------------------------------------------------------
computer daresbury.ac.uk (also known as dl.ac.uk):
-------------------------------------------------

To subscribe and unsubscribe to/from the BIOSCI lists, you need to
specify the full USENET newsgroup name with "bionet-news." prepended.
The USENET newsgroup names are listed in the BIOSCI Information sheet
on the Web at http://www.bio.net/.  For the METHODS-AND-REAGENTS list
the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the
appropriate commands are

    sub bionet-news.bionet.molbio.methds-reagnts

    unsub bionet-news.bionet.molbio.methds-reagnts

These commands are included in a message addressed to mxt@dl.ac.uk,
NOT to the newsgroup mailing addresses.  As usual, include the text in
the body of the message as text on the Subject: line is ignored.

To unsubscribe from all the lists at the UK node, use

    unsub bionet-news

Please note that if the address in the list is different than the one
in your mail message header, you will not be able to unsubscribe by
this method. If you have problems, please mail biosci@daresbury.ac.uk.


4) The BIOSCI user address and research interest directory.
-----------------------------------------------------------
Please take this opportunity to add your name, address, and research
interest information to the BIOSCI User Address Database if you have
not already done so.

You can fill out the address form directly through our Web page at URL
http://www.bio.net/adrform.html.

The address database is reindexed nightly for WWW access (the URL is
http://www.bio.net/).  If you are not directly on the Internet but can
reach it by e-mail, please use our waismail server to access the user
directory.  waismail use is described above.  You can also request a
user address form by e-mail from biosci-help@net.bio.net.

Please check your database entry from time-to-time to see if your
address information is still up-to-date.  Because of our limited
personnel resources, we ask that you resubmit a *complete* form to
revise your entry; we only replace complete entries and do not have
resources to edit old forms.




From owner-maize@net.bio.net Thu Apr 16 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: "W. Allen Miller" <wamiller@iastate.edu>
Newsgroups: bionet.maize
Subject: POSTDOCTORAL POSITION: TRANSLATION IN PLANTS
Date: 17 Apr 1998 09:06:00 -0700
Organization: Iowa State University
Lines: 30
Sender: daemon@net.bio.net
Approved: msachs@uiuc.edu
Distribution: world
Message-ID: <35377C21.598F2EAA@iastate.edu>
Reply-To: wamiller@iastate.edu
NNTP-Posting-Host: net.bio.net

POSTDOCTORAL POSITION: TRANSLATION IN MAIZE

Join a dynamic lab that is active in studying RNA structure and function

in virus replication and translation. Your job would be to characterize
and optimize a viral mRNA sequence that gives efficient cap-independent
translation in plants with the goal of using it for high-level gene
expression in transgenic maize.

Experience in RNA and/or plant molecular biology desired.

Start: Summer or Fall. Duration: At least two years.

See: http://www.public.iastate.edu/~wamiller/

Contact: W. Allen Miller
Associate Professor
351 Bessey Hall
Iowa State Univesity
Ames, IA 50011
wamiller@iastate.edu

Iowa State University, already a leader in plant genetics, is committed
to being a top institution in plant molecular biology (see
http://www.ag.iastate.edu/plantsci/plant.html). Outstanding nucleic
acids facilities, plant transformation facilities, and other
biotechnology facilities are well-established.




From owner-maize@net.bio.net Thu Apr 23 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: "Yongli Xiao" <yxiao@iastate.edu>
Newsgroups: bionet.maize
Subject: Ac PCR
Date: 24 Apr 1998 11:53:04 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 12
Sender: daemon@net.bio.net
Approved: msachs@uiuc.edu
Distribution: world
Message-ID: <199804241805.NAA26896@isua1.iastate.edu>
NNTP-Posting-Host: net.bio.net

Hi! Everyone,

I am graduate student at Iowa State Univ. I want to use long PCR kit to
amplify maize genomic DNA which contain Ac element. I wonder if somebody
had experience amplyfing Ac.

Thank you in advance!

Yongli




From owner-maize@net.bio.net Thu Apr 23 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: Tobias Baskin <baskin@biosci.mbp.missouri.edu>
Newsgroups: bionet.maize
Subject: question for spanish speakers
Date: 24 Apr 1998 12:57:31 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 20
Sender: daemon@net.bio.net
Approved: msachs@uiuc.edu
Distribution: world
Message-ID: <v03020925b166ab37f379@[128.206.162.73]>
Reply-To: Tobias Baskin <baskin@biosci.mbp.missouri.edu>, maize@net.bio.net
NNTP-Posting-Host: net.bio.net

Greetings,
	  Can any one tell me what the spanish word "acuminados" means when
used in reference to maize? I was reading something in spanish that
referred to "granos acuminados", but I can't find acuminados is my
dictionary.

	Thanks,
		Tobias Baskin

      _      ____   ^      __   ____   Tobias I. Baskin
     /  \   /      / \    /   \ \            University ofMissouri
    /   |  /      /   \   \      \              BiologicalSciences
   /___/  /__    /___  \   \      \__            109 Tucker Hall
  /      /      /       \   \      \            Columbia, MO 65211-7400 USA
 /      /      /         \   \      \          voice: 573-882-0173
/      /____  /           \   \__/   \____ fax: 573-882-0123





From owner-maize@net.bio.net Thu Apr 23 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: Rich Jorgensen <raj@ag.arizona.edu>
Newsgroups: bionet.maize
Subject: Postdoctoral Position Available
Date: 24 Apr 1998 11:55:39 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 39
Sender: daemon@net.bio.net
Approved: msachs@uiuc.edu
Distribution: world
Message-ID: <v03007806b16687c111a3@[128.196.152.134]>
NNTP-Posting-Host: net.bio.net

A postdoctoral position is available to study gene silencing, with emphasis
on hierarchies of homology-based control of anthocyanin gene expression and
resetting/remodeling of chromatin-based states of gene expression in
petunia and arabidopsis.

Applicants should send CV, statement of research accomplishments and
aspirations, and names of three references to Rich Jorgensen, Department of
Plant Sciences, University of Arizona (http://ag.arizona.edu/PLS/ ).
Minimum qualifications: Ph.D. in molecular genetics or related discipline.
Preferred qualifications: Experience in epigenetics, chromatin, and
transgene silencing in plants. Applications accepted until the position is
filled. University of Arizona is an EEO/AA Employer-M/W/D/V.
http://www.arizona.edu/

Recent publications (reprints available on request):
Que, Q, HY Wang, JJ English, RA Jorgensen. 1997. The frequency and degree
of cosuppression by sense chalcone synthase transgenes are dependent on
transgene promoter strength and are reduced by premature nonsense codons in
the transgene coding sequence. Plant Cell 9:1357-1368.
Que, Q, and RA Jorgensen. 1998. Homology-based control of gene expression
patterns in transgenic petunia flowers. Developmental Genetics 22:100-109.
Jorgensen, RA, RG Atkinson, RLS Forster, and WJ Lucas. 1998. An RNA-based
information superhighway in plants? Science 279:1486-1487.
Que, Q, HY Wang, and RA Jorgensen. 1998. Distinct patterns of pigment
suppression are produced by allelic sense and antisense chalcone synthase
transgenes in petunia flowers. Plant Journal, 13: 401-409.
Jorgensen, RA. 1995. Cosuppression, flower color patterns, and metastable
gene expression states. Science 268: 686-691.


Rich Jorgensen
Dept. of Plant Sciences
Forbes 303
University of Arizona
Tucson, AZ  85721-0036  USA
raj@ag.arizona.edu




From owner-maize@net.bio.net Fri Apr 24 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: "White, Jeffrey" <J.WHITE@CGNET.COM>
Newsgroups: bionet.maize
Subject: RE: granos acuminados
Date: 25 Apr 1998 08:30:15 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 14
Sender: daemon@net.bio.net
Approved: msachs@uiuc.edu
Distribution: world
Message-ID: <D7928EB64BE3D01182810000F803B3A52830E6@201.CIMMYT.CGIAR.ORG>
Reply-To: maize@net.bio.net
NNTP-Posting-Host: net.bio.net

Hello Tobias and others,

A more precise translation is "acuminate":

said of an acute apex whose sides are somewhat concave and taper to a
protracted point

>From Lawrence, Taxonomy of Vascular Plants.

-- Jeff White





From owner-maize@net.bio.net Fri Apr 24 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: pstinard@uiuc.edu (Phil Stinard)
Newsgroups: bionet.maize
Subject: granos acuminados
Date: 25 Apr 1998 07:54:09 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 10
Sender: daemon@net.bio.net
Approved: msachs@uiuc.edu
Distribution: world
Message-ID: <v01530500b166e415876d@[130.126.27.177]>
Reply-To: maize@net.bio.net
NNTP-Posting-Host: net.bio.net

Dear Tobias,

According to the Vox Dictionary of the Spanish Language, "acuminado" means
"pointed."  Therefore, "granos acuminados" would be "pointed kernels".

--Phil Stinard





From owner-maize@net.bio.net Sat Apr 25 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: SURESH NAIK <snaik@kelvin.ncl.res.in>
Newsgroups: bionet.maize
Subject: Re: question for spanish speakers
Date: 26 Apr 1998 12:09:32 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 32
Sender: daemon@net.bio.net
Approved: msachs@uiuc.edu
Distribution: world
Message-ID: <Pine.LNX.3.96.980426085610.23471B-100000@kelvin.ncl.res.in>
Reply-To: maize@net.bio.net
NNTP-Posting-Host: net.bio.net


Dear Tobias:

If I can understand the words one by one, Granos has to do with grains and
the word `acuminados' can be understood to do something with the word
`ACUTE' which means pointed.  Althouogh I don't understand much of
Spanish, combining both should have something to do with the shape of the
grains.  Possible that they call maize "granos acuminados" because of its
shape:-pointed towards one end.

Best
SURESH

+++++++++++++++++++++++++++++++++++
SURESH NAIK
Plant Molecular Biology Unit
Division of Biochemical Sciences
National Chemical Laboratory
Pune 411 008
INDIA

FAX: +91-212-338234
Email: snaik@kelvin.ncl.res.in

"ONLY WHEN THE LAST TREE HAS DIED &
THE LAST RIVER POISONED & THE LAST
FISH CAUGHT WILL WE REALIZE WE
CAN'T EAT MONEY"
+++++++++++++++++++++++++++++++++++++




From owner-maize@net.bio.net Sun Apr 26 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: Pierre Lejeune <Pierre.Lejeune@ulg.ac.be>
Newsgroups: bionet.maize
Subject: Re: question for spanish speakers
Date: 27 Apr 1998 06:27:42 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 24
Sender: daemon@net.bio.net
Approved: msachs@uiuc.edu
Distribution: world
Message-ID: <3.0.1.32.19980427100012.006a4dc0@pop3.mailst.ulg.ac.be>
Reply-To: maize@net.bio.net
NNTP-Posting-Host: net.bio.net


In french, 'acumine' means : terminated by a thin and elongated tip.

Cheers,

Pierre

--------------------------------------------
Pierre LEJEUNE

Universite de LIEGE
Dept. Biologie Vegetale
Service de Physiologie
Sart Tilman B22
B4000 LIEGE
BELGIUM

Tel: (32) 4 366 38 36
Fax : (32) 4 366 38 31
Email : Pierre.Lejeune@ulg.ac.be
-------------------------------------------------




From owner-maize@net.bio.net Sun Apr 26 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: Tobias Baskin <baskin@biosci.mbp.missouri.edu>
Newsgroups: bionet.maize
Subject: granos acuminados--Gracias!
Date: 27 Apr 1998 08:12:02 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 22
Sender: daemon@net.bio.net
Approved: msachs@uiuc.edu
Distribution: world
Message-ID: <v03020905b16a526cf47f@[128.206.162.73]>
NNTP-Posting-Host: net.bio.net

Greetings,

Many thanks to all those who answered my querey about the meaning
of granos acuminados.

And if anyone else cares, the bottomline English equivalent is
"pointed kernels".

	Muchas Gracias,
			Tobias Baskin

      _      ____   ^      __   ____   Tobias I. Baskin
     /  \   /      / \    /   \ \            University ofMissouri
    /   |  /      /   \   \      \              BiologicalSciences
   /___/  /__    /___  \   \      \__            109 Tucker Hall
  /      /      /       \   \      \            Columbia, MO 65211-7400 USA
 /      /      /         \   \      \          voice: 573-882-0173
/      /____  /           \   \__/   \____ fax: 573-882-0123





From owner-maize@net.bio.net Mon Apr 27 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: Hillmare <Hillmare@icon.co.za>
Newsgroups: bionet.maize
Subject: use for maize waste products?
Date: 28 Apr 1998 06:50:25 -0700
Organization: NCM
Lines: 9
Sender: daemon@net.bio.net
Approved: msachs@uiuc.edu
Distribution: world
Message-ID: <3545DC7A.6325@icon.co.za>
Reply-To: Hillmare@icon.co.za
NNTP-Posting-Host: net.bio.net

Could you tell me if there is alternative uses for the waste products of
white maize after it has been milled, other than for animal feed.


Thank You
H Schulze

E-mail hillmare@icon.co.za


From owner-maize@net.bio.net Tue Apr 28 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: Mike Foley <foley@btny.purdue.edu>
Newsgroups: bionet.maize
Subject: Postdoctoral position
Date: 29 Apr 1998 06:32:13 -0700
Organization: Purdue University
Lines: 17
Sender: daemon@net.bio.net
Approved: msachs@uiuc.edu
Distribution: world
Message-ID: <3546E2AF.2A77@btny.purdue.edu>
Reply-To: foley@btny.purdue.edu
NNTP-Posting-Host: net.bio.net

Postdoctoral Position
Purdue University, West Lafayette, Indiana

A postdoctoral position is available to study the genetic and
environmental basis for dormancy in cereal grain seeds.  We have
developed recombinant inbred (RI) lines and other population segregating
for dormancy.  The overall objectives are to determine the germination
phenotype and genotype of RI lines under various environmental
conditions and identify molecular markers for dormancy QTL.  These
objectives are consistent with the goal of cloning dormancy QTL.
Candidates should have a background in molecular genetics.  Previous
experience in genetics, statistics and seed biology is desirable.  Send
a curriculum vitae and the names, telephone numbers, and e-mail
addresses of three references to Dr. Michael Foley, Department of Botany
and Plant Pathology, Purdue University, West Lafayette, IN 47907-1155;
fax 765-494-0363; e-mail foley@btny.purdue.edu.


From owner-maize@net.bio.net Wed Apr 29 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: Wendy Shirilla <Wendy@AdvInfoC.com>
Newsgroups: bionet.maize
Subject: Info. from the 15TH EUCARPIA CONGRESS MAIZE-SORGHUM
Date: 30 Apr 1998 06:56:40 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 41
Sender: daemon@net.bio.net
Approved: msachs@uiuc.edu
Distribution: world
Message-ID: <01BD72A2.EF039620@GAT2295.AdvInfoC.com>
NNTP-Posting-Host: net.bio.net


Maize Database
db_request@teosinte.agron.missouri.edu
[Forwarding the accompanying request to the maize net.  Can someone help
with this?    -Ed Coe]

Dear Sir/Madam:

I am interested in obtaining a copy of the following paper:

     15TH EUCARPIA CONGRESS MAIZE-SORGHUM
     1990 4-8 June, 277-288
     Use of NIR Prediction of Digestibility in a Breeding Program for Silage
     Maize.  Ronsin, T.

I have been unable to locate this document through conventional sources.  I
found the name of your organization connected with a Eucarpia congress for
a different year. Would you please supply me with a copy of this paper or
direct me with further information to a source where I might be able to
locate it?

PLEASE use our DEPARTMENT NUMBER (at the bottom) for any responses and for
billing.  We must have this number in order to locate our original order
and to process payment.

**If the cost exceeds $15.00, please send a Price Quote for further
approval.  Otherwise, please send the paper along with an invoice.

Thank you for your assistance!

Kind regards,

Wendy Shirilla
Dept: 1701479

Advanced Information Consultants
41575 Joy Rd.
Canton, MI  48187 USA
734-459-9090 (phone)
734-459-8990 (fax)
Wendy@AdvInfoC.com

