From owner-markers@hgmp.mrc.ac.uk Thu Feb 1 12:31:59 2001 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 7A2FA17AF5; Thu, 1 Feb 2001 12:31:59 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6033) id D17B517AC4; Thu, 1 Feb 2001 12:31:57 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 2482217B1A; Wed, 31 Jan 2001 14:59:38 +0000 (GMT) Received: from snipp.uninett.no (snipp.uninett.no [128.39.3.166]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id E671E415F4 for ; Wed, 31 Jan 2001 14:59:28 +0000 (GMT) Received: (qmail 4626 invoked by uid 121); 31 Jan 2001 14:59:26 +0000 (GMT) To: bionet-genomes-markers@net.bio.net From: "Thomas Moen" Newsgroups: bionet.genomes.markers Subject: More on missing AFLP bands Organization: UNINETT news service Message-ID: <95998e$4ge$1@snipp.uninett.no> X-Trace: snipp.uninett.no 980953166 4622 128.39.177.199 (31 Jan 2001 14:59:26 GMT) X-Complaints-To: news-abuse@uninett.no X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 5.00.2615.200 X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2615.200 Date: Thu, 1 Feb 2001 12:31:57 +0000 (GMT) Sender: owner-markers@hgmp.mrc.ac.uk Precedence: bulk Hi there! This is a follow up on an earlier message sent by Martin Parr, and the reply of Alastair Hamilton. Concerning those weak bands that appear only on the autoradiographs; does anyone know if they can be trusted (segregation etc.), or if they are just artifacts? Why are some bands weaker than others anyway...? Thanks! Thomas thomas.moen@akvaforsk.nlh.no From owner-markers@hgmp.mrc.ac.uk Thu Feb 1 12:32:05 2001 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 92E2017B15; Thu, 1 Feb 2001 12:32:05 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6033) id 3241F17AF5; Thu, 1 Feb 2001 12:32:03 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 1AEFC17C01; Wed, 31 Jan 2001 20:47:23 +0000 (GMT) To: bionet-genomes-markers@net.bio.net From: yoder@vegmail.ucdavis.edu ("Yoder, John") Newsgroups: bionet.genomes.markers Subject: post doc in plant-plant signaling Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <16D03631929FD311BC5D009027D0CBB24426AF@vegmail.ucdavis.edu> MIME-Version: 1.0 Content-Type: text/plain; charset="iso-8859-1" X-Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id D365517B0C; Wed, 31 Jan 2001 20:47:21 +0000 (GMT) X-Received: from vegmail.ucdavis.edu (vegmail.ucdavis.edu [169.237.8.2]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 9B31D41659 for ; Wed, 31 Jan 2001 20:47:14 +0000 (GMT) X-Received: by vegmail.ucdavis.edu with Internet Mail Service (5.5.2650.21) id ; Wed, 31 Jan 2001 12:52:07 -0800 X-To: "'markers@net.bio.net'" X-Mailer: Internet Mail Service (5.5.2650.21) Date: Thu, 1 Feb 2001 12:32:03 +0000 (GMT) Sender: owner-markers@hgmp.mrc.ac.uk Precedence: bulk Post doctoral position is available to investigate the molecular genetics of plant-plant interactions. We are interested in defining the signaling pathways that regulate how plants grow in the vicinity of other plants. The goal of the post doctoral research is to assign biochemical and in vivo functions to transcripts hypothesized as signal mediators between plants (Matvienko et al., 2001 Plant J. 25(3) 1-15. More details of our research can be found at . The successful candidate will have extensive expertise in molecular biology, yeast genetics and/or biochemistry. Experience in bioinformatics highly preferred. Two year appointment with salary commensurate with qualifications. Applicants should send a letter of interest, C.V., and a list of references (including telephone numbers and e-mail addresses): John I. Yoder, Prof. Department of Vegetable Crops University of California, Davis Davis, CA. 95616 530-752-1741 jiyoder@ucdavis.edu --- From owner-markers@hgmp.mrc.ac.uk Thu Feb 8 13:14:51 2001 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 1174817AD7; Thu, 8 Feb 2001 13:14:50 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6033) id 3E77917AB7; Thu, 8 Feb 2001 13:14:49 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 3632817C09; Wed, 7 Feb 2001 19:25:41 +0000 (GMT) To: bionet-genomes-markers@net.bio.net From: gordonr@Ms.UManitoba.CA (Richard Gordon) Newsgroups: bionet.genomes.markers Subject: Finding Reprint Authors' E-Mail Addresses Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" ; format="flowed" X-Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 6005917C06; Wed, 7 Feb 2001 19:25:37 +0000 (GMT) X-Received: from naos.cc.umanitoba.ca (naos.cc.umanitoba.ca [130.179.16.122]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 2E6FA415DF for ; Wed, 7 Feb 2001 19:25:16 +0000 (GMT) X-Received: from [24.108.134.118] (24-108-134-118.ivideon.com [24.108.134.118]) by naos.cc.umanitoba.ca (8.9.0/8.9.0) with ESMTP id NAA17641 for ; Wed, 7 Feb 2001 13:25:10 -0600 (CST) X-Sender: gordonr@mail.ms.umanitoba.ca X-To: markers@net.bio.net Date: Thu, 8 Feb 2001 13:14:49 +0000 (GMT) Sender: owner-markers@hgmp.mrc.ac.uk Precedence: bulk Eric Cowdrey and I have put together a web page that makes it relatively easy to find the e-mail addresses of academic colleagues, for the purpose of requesting reprints: Finding Reprint Authors' E-Mail Addresses http://www.umanitoba.ca/faculties/medicine/radiology/search/searchindex.html There are no a d v e r t i s e m e n t s or costs. If you use it, please let us know of omissions, corrections, or improvements you'd like, and forward it to your colleagues. -- Dr. Richard Gordon, Radiology, University of Manitoba, HSC Rm. GA216, 820 Sherbrook St. Winnipeg R3A 1R9 Canada Adjunct: Electrical & Computer Engineering, Exec Member: CSTB, CARRF, IEEE-EMBS phone:(204)789-3828, fax:(204)787-2080, e-mail: GordonR@ms.umanitoba.ca New book: The Hierarchical Genome & Differentiation Waves: Novel Unification of Development, Genetics & Evolution: http://www.wspc.com.sg/books/lifesci/2755.html Finding Reprint Authors' E-Mail Addresses: http://www.umanitoba.ca/faculties/medicine/radiology/search/searchindex.html --- From owner-markers@hgmp.mrc.ac.uk Thu Feb 15 23:36:35 2001 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 26FB917C09; Thu, 15 Feb 2001 23:36:35 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6033) id 0001017B6B; Thu, 15 Feb 2001 23:36:33 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id CEFAB17AB6; Thu, 15 Feb 2001 14:16:47 +0000 (GMT) To: bionet-genomes-markers@net.bio.net From: oss601@bangor.ac.uk (Halina Sobolewska) Newsgroups: bionet.genomes.markers Subject: Re. more on missing AFLP bands Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <3A8BE47F.632B27D3@bangor.ac.uk> MIME-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id ADD2317A62; Thu, 15 Feb 2001 14:16:19 +0000 (GMT) X-Received: from pragmatix.bangor.ac.uk (pragmatix.bangor.ac.uk [147.143.2.14]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 7B4B541630 for ; Thu, 15 Feb 2001 14:15:36 +0000 (GMT) X-Received: from kirk.bangor.ac.uk (kirk [147.143.2.86]) by pragmatix.bangor.ac.uk (8.9.3+Sun/8.9.3) with ESMTP id OAA08218 for ; Thu, 15 Feb 2001 14:15:29 GMT X-Received: from bangor.ac.uk (adcr105 [147.143.5.105]) by kirk.bangor.ac.uk (8.9.3+Sun/8.9.3) with ESMTP id OAA16436 for ; Thu, 15 Feb 2001 14:15:28 GMT X-Mailer: Mozilla 4.76 [en] (Win98; U) X-Accept-Language: en X-To: markers@net.bio.net Date: Thu, 15 Feb 2001 23:36:33 +0000 (GMT) Sender: owner-markers@hgmp.mrc.ac.uk Precedence: bulk Hi Thomas, I think it's fairly inevitable that PCR amplification of multiple templates is going to amplify some fragments more efficiently than others. Further, because this is PCR, the tiniest variations in amplification efficiency during early cycles will translate into enormous differences in the concentration of different amplicons by the end. Of course a PCR explanation for fainter bands this doesn't rule out artefacts, though it isn't something I have seen. Obviously, to discriminate between background noise and faint bands it's necessary to set a threshold. Providing bands that are close to this threshold amplify consistently then they should be trustworthy. Life, however, is not just or fair and I have seen published work that reports considerable differences in the relative intensity of different AFLP bands between duplicates, though, again, it's not something I have experienced. I'd be very keen to hear from anyone who has. I could dig out some references on this if that would be helpful Regards Alastair Alastair Hamilton From: Thomas Moen (thomas.moen@akvaforsk.nlh.no) >>Hi there! >>This is a follow up on an earlier message sent by Martin Parr, and the reply >>of Alastair Hamilton. Concerning those weak bands that appear only on the >>autoradiographs; does anyone know if they can be trusted (segregation etc.), >>or if they are just artifacts? Why are some bands weaker than others >>anyway...? >>Thanks! >>Thomas --- From owner-markers@hgmp.mrc.ac.uk Mon Feb 19 18:06:21 2001 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id F004317B5E; Mon, 19 Feb 2001 18:06:20 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6033) id 8400517B5D; Mon, 19 Feb 2001 18:06:19 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id DDDE517AF6; Mon, 19 Feb 2001 04:45:07 +0000 (GMT) To: bionet-genomes-markers@net.bio.net From: bein@edu.21cn.com (bein) Newsgroups: bionet.genomes.markers Subject: how to search cSNP? Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <77452BD01388.AAA4F65@letter.gzsums.edu.cn> Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Content-Transfer-Encoding: 7bit X-Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 6BB9317AEA; Mon, 19 Feb 2001 04:45:06 +0000 (GMT) X-Received: from info.gzsums.edu.cn (info.gzsums.edu.cn [202.116.96.34]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id D14A1415E5 for ; Mon, 19 Feb 2001 04:44:59 +0000 (GMT) X-Received: from letter.gzsums.edu.cn (localhost [127.0.0.1]) by info.gzsums.edu.cn (8.9.1b+Sun/8.9.1) with ESMTP id MAA22131 for ; Mon, 19 Feb 2001 12:36:53 +0800 (CST) X-Received: from cisums ([202.116.124.184]) by letter.gzsums.edu.cn (Netscape Messaging Server 3.6) with SMTP id AAA4F65 for ; Mon, 19 Feb 2001 12:50:00 +0800 X-To: "markers@net.bio.net" X-mailer: FoxMail 3.0 beta 1 [cn] Date: Mon, 19 Feb 2001 18:06:19 +0000 (GMT) Sender: owner-markers@hgmp.mrc.ac.uk Precedence: bulk Dear colleages: Could anyone kindly tell me how to search cSNP within a cytogenetic bank region, say all cSNPs within 3p21, or between two microsatellites. Thank you very much. yours Benjamin Feng Sun Yat-sen University of Medical sciences Guangzhou, 510060 P.R.China --- From owner-markers@hgmp.mrc.ac.uk Wed Feb 21 11:08:12 2001 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 2AA8317AF0; Wed, 21 Feb 2001 11:08:12 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6033) id 0A6B717AEB; Wed, 21 Feb 2001 11:08:10 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 42F4717B7E; Tue, 20 Feb 2001 17:41:54 +0000 (GMT) To: bionet-genomes-markers@net.bio.net From: dbell@qnis.net ("Deanne Bell") Newsgroups: bionet.genomes.markers Subject: software packages Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <200102201743.f1KHh6i18706@ns.qnis.net> MIME-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 7bit X-Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 19AA917B7C; Tue, 20 Feb 2001 17:41:50 +0000 (GMT) X-Received: from ns.qnis.net (ns.qnis.net [206.171.190.2]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id CCB65415DA for ; Tue, 20 Feb 2001 17:41:32 +0000 (GMT) X-Received: from gateway2000 (dialup25.Fresno.qnis.net [207.104.115.25]) by ns.qnis.net (8.11.0/8.11.0) with ESMTP id f1KHh6i18706 for ; Tue, 20 Feb 2001 09:43:06 -0800 (PST) X-To: X-MSMail-Priority: Normal X-Priority: 3 X-Mailer: Microsoft Internet Mail 4.70.1155 Date: Wed, 21 Feb 2001 11:08:10 +0000 (GMT) Sender: owner-markers@hgmp.mrc.ac.uk Precedence: bulk Dear Alastair You mentioned that there are several software packages on the market which can import ABI densitometric data - would you be so kind as to recommend some websites or names as I have only found those that are mutally exclusive of ABI. Thank you for your time Deanne Bell Molecular Markers Lab Technician USDA Agricultural Research Service 2021 S. Peach Ave. Fresno, CA 93727 e-mail: dbell@qnis.net phone: 559 453-3170 fax: 559 453-3088 --- From owner-markers@hgmp.mrc.ac.uk Wed Feb 21 11:08:22 2001 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id A1FC217AEB; Wed, 21 Feb 2001 11:08:21 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6033) id 8223917AE9; Wed, 21 Feb 2001 11:08:20 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id B394017B7E; Tue, 20 Feb 2001 17:41:50 +0000 (GMT) To: bionet-genomes-markers@net.bio.net From: dbell@qnis.net ("Deanne Bell") Newsgroups: bionet.genomes.markers Subject: subscribe questions Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <200102201743.f1KHh4i18703@ns.qnis.net> MIME-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 7bit X-Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 7FC4417B7C; Tue, 20 Feb 2001 17:41:46 +0000 (GMT) X-Received: from ns.qnis.net (ns.qnis.net [206.171.190.2]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id CA93F41656 for ; Tue, 20 Feb 2001 17:41:30 +0000 (GMT) X-Received: from gateway2000 (dialup25.Fresno.qnis.net [207.104.115.25]) by ns.qnis.net (8.11.0/8.11.0) with ESMTP id f1KHh4i18703 for ; Tue, 20 Feb 2001 09:43:04 -0800 (PST) X-To: X-MSMail-Priority: Normal X-Priority: 3 X-Mailer: Microsoft Internet Mail 4.70.1155 Date: Wed, 21 Feb 2001 11:08:20 +0000 (GMT) Sender: owner-markers@hgmp.mrc.ac.uk Precedence: bulk I just sent a "subscribe markers" e-mail to the majordomo@hgmp.mrc.ac.uk - Is this how I can sign up for this newsgroup? I do not have a Usenet interface. I was referred to this newsgroup by Martin Parr, but I did not find the Markers group listed on the bio-net home / information page. Deanne Bell Molecular Markers Lab Technician USDA Agricultural Research Service 2021 S. Peach Ave. Fresno, CA 93727 e-mail: dbell@qnis.net phone: 559 453-3170 fax: 559 453-3088 ---