Janel Laidman
Department of Chemistry and Biochemistry
California State University, Los Angeles

email jwheele@calstatela.edu --------------6ED4F9BA10B4FDE15B5E4F4F-- From owner-methods@hgmp.mrc.ac.uk Tue Nov 2 18:15:39 1999 Return-Path: Delivered-To: methods-archive@hgmp.mrc.ac.uk Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 8F3BA17A58; Tue, 2 Nov 1999 18:15:37 +0000 (GMT) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id SAA04502 for ; Tue, 2 Nov 1999 18:15:26 GMT Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id SAA02133 for methods-list@hgmp.mrc.ac.uk; Tue, 2 Nov 1999 18:15:25 GMT X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: fernando Subject: Synthetic VH + VL scFv Library X-Newsgroups: bionet.molbio.methds-reagnts Message-ID: <0f9f8e5a.68157e17@usw-ex0106-048.remarq.com> Lines: 15 Bytes: 294 X-Originating-Host: 193.136.151.73 Organization: http://www.remarq.com: The World's Usenet/Discussions Start Here X-Wren-Trace: eF14UFFID0UOG0xHVhxSUEAATllKXRJYV3FbXEVdRVNbVRhIWlNYRxENAAsdTB0cEgAEAwRVbg== Date: Tue, 02 Nov 1999 10:01:03 -0800 X-Complaints-To: wrenabuse@remarq.com X-Trace: WReNphoon3 941565933 10.0.2.44 (Tue, 02 Nov 1999 10:05:33 PST) To: methods@hgmp.mrc.ac.uk Sender: owner-methods@hgmp.mrc.ac.uk Precedence: bulk Hi all, I looking for a Synthetic VH + VL scFv Library for my research, do you know where can I ask for one? thanks, Fernando * Sent from RemarQ http://www.remarq.com The Internet's Discussion Network * The fastest and easiest way to search and participate in Usenet - Free! From owner-methods@hgmp.mrc.ac.uk Tue Nov 2 18:45:37 1999 Return-Path: Delivered-To: methods-archive@hgmp.mrc.ac.uk Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 4392017A58; Tue, 2 Nov 1999 18:45:35 +0000 (GMT) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id SAA05846 for ; Tue, 2 Nov 1999 18:45:31 GMT Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id SAA02502 for methods-list@hgmp.mrc.ac.uk; Tue, 2 Nov 1999 18:45:29 GMT X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: kaghoram@garnet.acns.fsu.edu (Karthik Aghoram) X-Newsgroups: bionet.molbio.methds-reagnts Subject: Re: 5' RACE looking for a generic protocol Date: 2 Nov 1999 18:28:33 GMT Organization: Florida State University Lines: 41 Message-ID: <7vnagh$bhp$1@news.fsu.edu> References: <381F2718.5D64C8C0@calstatela.edu> X-Newsreader: TIN [version 1.2 PL2] To: methods@hgmp.mrc.ac.uk Sender: owner-methods@hgmp.mrc.ac.uk Precedence: bulk I used GIBCO's kit (I solemnly affirm that I am not affiliated to them) with good success on guard-cell material. The key, of course, is in primer design. You can find their protocol on www.lifetech.com. Others have reported success with Clontech, but it did nothing in my hands. Good luck. Mahaanole Vellanoweth's Lab (ddeloss@calstatela.edu) wrote: : --------------6ED4F9BA10B4FDE15B5E4F4F : Content-Type: text/plain; charset=us-ascii : Content-Transfer-Encoding: 7bit : I'm looking for a generic (i.e. not a kit) protocol for 5' RACE (for : plant materials). If anyone can point me in the direction of one I'd : appreciate it. Thanks. : Janel Laidman : Department of Chemistry and Biochemistry : California State University, Los Angeles : email jwheele@calstatela.edu : --------------6ED4F9BA10B4FDE15B5E4F4F : Content-Type: text/html; charset=us-ascii : Content-Transfer-Encoding: 7bit : : I'm looking for a generic (i.e. not a kit) protocol for 5' RACE : (for plant materials). If anyone can point me in the direction of : one I'd appreciate it. Thanks. :

Janel Laidman :
Department of Chemistry and Biochemistry :
California State University, Los Angeles :

email jwheele@calstatela.edu : --------------6ED4F9BA10B4FDE15B5E4F4F-- From owner-methods@hgmp.mrc.ac.uk Tue Nov 2 18:45:37 1999 Return-Path: Delivered-To: methods-archive@hgmp.mrc.ac.uk Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 921DD17AB2; Tue, 2 Nov 1999 18:45:36 +0000 (GMT) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id SAA05847 for ; Tue, 2 Nov 1999 18:45:32 GMT Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id SAA02507 for methods-list@hgmp.mrc.ac.uk; Tue, 2 Nov 1999 18:45:30 GMT X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: gbaker@U.ARIZONA.EDU ("Gregory L. Baker") X-Newsgroups: bionet.molbio.methds-reagnts Subject: Nonradiocative Northern Method Recomendation Date: 2 Nov 1999 10:21:23 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 38 Distribution: world Message-ID: <000701bf255e$12c7c9e0$109dc480@Liver-one.cba.arizona.edu> To: methods@hgmp.mrc.ac.uk Sender: owner-methods@hgmp.mrc.ac.uk Precedence: bulk This is a multi-part message in MIME format. ------=_NextPart_000_0004_01BF2523.65CA40E0 Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Our lab would like to get away from using 32P for northerns. A few = years ago we had tried to perform non-rad northerns in the lab with = little sucess. I was wondering if anyone has had any recent experience = using no-rad probes for northerns and could suggest either a method, = vendor, or web site to help get us started. Thanks in advance. ------=_NextPart_000_0004_01BF2523.65CA40E0 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable

Our lab would like to get away from using 32P for=20 northerns. A few years ago we had tried to perform non-rad = northerns in=20 the lab with little sucess. I was wondering if anyone has had any = recent=20 experience using no-rad probes for northerns and could suggest either a=20 method, vendor, or web site to help get us started. Thanks in = advance.