From owner-molluscs@net.bio.net Thu Mar 07 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Bertrand COLLET Newsgroups: bionet.molbio.molluscs Subject: (no subject) Date: 8 Mar 1996 10:35:59 -0800 Organization: IFREMER Lines: 12 Sender: daemon@net.bio.net Approved: boulding@uoguelph.ca Distribution: world Message-ID: <4hpmno$91q@ys.ifremer.fr> NNTP-Posting-Host: net.bio.net Hello, I work on oyster genetics and I try to extract DNA from lyophilised tissus (gill or mantle). I used the classical phenol-chloroform procedure which is ok with DNA extracted from biopsed tissu but failed with DNA from lyophilised tissu. Is anybody know a good method for DNA extraction ? Thanks From owner-molluscs@net.bio.net Mon Mar 11 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Louise.English@ml.csiro.au (Louise English) Newsgroups: bionet.molbio.molluscs Subject: oyster mtDNA primers Date: 12 Mar 1996 15:51:00 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Approved: boulding@uoguelph.ca Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Could anyone please tell me of any primers they have used to amplify up variable regions of mitochondrial DNA in oysters (or any other mollusc or bivalve)? I'm having some difficulty with this in Crassostrea gigas (Pacific or Japanese oyster). Louise English Phone:+61 02 325 379 CSIRO Division of Fisheries Email:Louise.English@ml.csiro.au GPO Box 1538 Fax:+61 02 325 485 Hobart Tas 7001