From owner-molluscs@net.bio.net Fri Jun 21 23:00:00 1996 Path: biosci!biosci!not-for-mail From: jgauley@sfu.ca (Julie Lynne Gauley) Newsgroups: bionet.molbio.molluscs Subject: marine invertebrates Date: 22 Jun 1996 08:38:20 -0700 Organization: Simon Fraser University Lines: 2 Sender: daemon@net.bio.net Approved: thay@uoguelph.ca Distribution: world Message-ID: <4qac3r$lkd@morgoth.sfu.ca> NNTP-Posting-Host: net.bio.net how are gastropods similar to foraminiferans? From owner-molluscs@net.bio.net Fri Jun 21 23:00:00 1996 Path: biosci!biosci!not-for-mail From: "Andrew G. McArthur" Newsgroups: bionet.molbio.molluscs Subject: PCR from Mucous (fwd) Date: 22 Jun 1996 08:38:02 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 52 Sender: daemon@net.bio.net Approved: thay@uoguelph.ca Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net >From the MOLLUSCA listserver... ---------- Forwarded message ---------- Date: Wed, 19 Jun 96 00:51:15 PDT From: Neville Sweijd To: Multiple recipients of list Subject: PCR from Mucous Hello I hope there are some people on this list that can help. I am seeking advice on how to extract DNA from mucous and/or whether it is possible to PCR directly from mucous. This is part of a forensic project on abalone where often the only clue is slime. We have a system that works well on all sorts of processed tissue but I am looking for a protocol to extract DNA from mucous. I presume that a CTAB, Phenol, Etoh type protocol would be the way to go. Any suggestions will be welcome. Thanks Neville Sweijd (nsweijd@botzoo.uct.ac.za) +-----------------------------+ | Neville Sweijd | | Department of Zoology | | University of Cape Town | | . * ~ ~ * , | | /'` \ | | /' /^^'\ , | | { | @/ |. | | {o `\ / ) | | {o '- ' ) | | {o +27-21-6503632 (ph) | | {o+27-21-6503301 (fax) | | {o ) | | {o ) | | {o ) | | {o )`\. | | {o ) `\. | | Nsweijd@botzoo.uct.ac.za | |ABNET: listerserver@uct.ac.za| | http://www.uct.ac.za/depts/ | | zoology/abnet/index.html | +-----------------------------+ From owner-molluscs@net.bio.net Sat Jun 22 23:00:00 1996 Path: biosci!biosci!not-for-mail From: "Andrew G. McArthur" Newsgroups: bionet.molbio.molluscs Subject: marine mollusc in situs (fwd) Date: 23 Jun 1996 09:38:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Approved: thay@uoguelph.ca Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net This one from the MOLLUSCA listserv... Date: Tue, 18 Jun 96 10:25:36 PDT From: "Sarah L. French" To: Multiple recipients of list Subject: marine mollusc in situs Hello, I am writing to inquire whether anyone has experience or protocols for in situ hybridization or in situ PCR in marine molluscs. Are there any particular problems associated with the increased ionic strength of seawater vs normal cell culture media? What about the intracellular milieu? Thanks for any info you can offer. Sarah French Department of Biology University of Virginia Charlottesville, VA 22901 slf3a@virginia.edu