From owner-molec-model@net.bio.net Wed Sep 02 23:00:00 1998 Path: biosci!SLIP.NET!grizzly From: grizzly@SLIP.NET (Michael Sherrell) Newsgroups: bionet.molec-model Subject: Sciex API III+ LC/MS/MS Date: 2 Sep 1998 18:52:16 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: <01BDD6A2.72CFA9E0@oak-pm2-40-232.dialup.slip.net> NNTP-Posting-Host: net.bio.net Sciex API III LC/MS/MS available at an excellent price: Vintage 1993 Upgraded to III+ API, ES, APCI sources Mass range to 2400 amu Always and currently under PE service contract New price was $539,000; price now: $79,000 For more details, please give me a call. Mike Sherrell Grizzly Analytical (USA) 707 887 2919/fax 707 887 9834 www.grizzlyanalytical.com From owner-molec-model@net.bio.net Wed Sep 02 23:00:00 1998 Path: biosci!NS1.NIMR.MRC.AC.UK!jsaldan From: jsaldan@NS1.NIMR.MRC.AC.UK (Jose Saldanha) Newsgroups: bionet.molec-model Subject: [ANNOUNCE] Humanization bY Design WWW Site Date: 3 Sep 1998 05:38:42 -0700 Organization: National Institute for Medical Research Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <35EE8D53.E3E13A30@nimr.mrc.ac.uk> NNTP-Posting-Host: net.bio.net [ANNOUNCE] Humanization bY Design WWW Site http://www.cryst.bbk.ac.uk/~ubcg07s http://mathbio.nimr.mrc.ac.uk/jsaldan The "Humanization bY Design" web site contains a background to antibody humanization as well as discussing various issues concerning the design of humanized antibodies for therapy and diagnosis. It includes information on the majority of humanized antibodies that have been published in the scientific literature. Text and sequence searching facilities are provided to find the humanized antibody of interest. From owner-molec-model@net.bio.net Thu Sep 03 23:00:00 1998 Path: biosci!news.stanford.edu!nntp.cs.ubc.ca!newsfeed.direct.ca!news-peer.sprintlink.net!news-backup-east.sprintlink.net!news.sprintlink.net!207.180.0.49!news.ici.net!not-for-mail From: "Hilton Evans" Newsgroups: alt.sci.nmr,bionet.molec-model,de.sci.biologie,de.sci.chemie,ncsc.chemistry Subject: Explore Molecular Geometry Date: 4 Sep 1998 11:43:38 GMT Organization: Chempen Software Lines: 4 Message-ID: <01bdd7f9$50795780$2d28b4cf@hfevans.ici.net> NNTP-Posting-Host: d-ma-superpop-45.ici.net X-Newsreader: Microsoft Internet News 4.70.1162 ChemPen Chemical Structure Drawing and Modeling Software http://home.ici.net/~hfevans/chempen3d.htm From owner-molec-model@net.bio.net Fri Sep 04 23:00:00 1998 Path: biosci!pravda.ucr.edu!awabi.library.ucla.edu!208.134.241.18!newsfeed.internetmci.com!144.212.95.13!nntprelay.mathworks.com!news.mathworks.com!uunet!uunet!in2.uu.net!news.ici.net!not-for-mail From: "Hilton Evans" Newsgroups: bionet.molec-model,de.sci.chemie,sci.chem,sci.chem.labware,sci.chem.organomet,sci.engr.chem,sci.research Subject: Molecular Mechanics Date: 5 Sep 1998 15:53:14 GMT Organization: Chempen Software Lines: 6 Message-ID: <01bdd8e5$5735dca0$2d29b4cf@hfevans.ici.net> NNTP-Posting-Host: d-ma-superpop-1-45.ici.net X-Newsreader: Microsoft Internet News 4.70.1162 ChemPen3D Chemical Drawing and Modeling Software http://home.ici.net/~hfevans/chempen3d.htm -- Hilton Evans From owner-molec-model@net.bio.net Sat Sep 05 23:00:00 1998 Path: biosci!agate!newsfeed.berkeley.edu!howland.erols.net!vixen.cso.uiuc.edu!dhardy From: dhardy@students.uiuc.edu (david joseph hardy) Newsgroups: bionet.molec-model Subject: Announce: NAMD 1.5 released Date: 6 Sep 1998 00:37:08 GMT Organization: University of Illinois at Urbana-Champaign Lines: 93 Message-ID: <6sslfk$dpq$1@vixen.cso.uiuc.edu> NNTP-Posting-Host: ux9.cso.uiuc.edu Announcing the release of NAMD version 1.5 ------------------------------------------ The Theoretical Biophysics group of the Beckman Institute at the University of Illinois would like to announce the availability of version 1.5 of NAMD, a high-performance molecular mechanics program for simulating large biomolecular systems on parallel and distributed computers. This software is being made available to the molecular modeling community free of charge and includes commented source code and extensive documentation. New in this version ------------------- * Added features include rigid bonds and moving harmonic restraints. * Updated user guide, also available in HTML form. * Modified to work with PVM 3.4 beta. * Enhanced performance by as much as 30%. * Modified to work with the latest version of DPMTA (2.7). * Included DPMTA source to make installation easier. * Simplified build process, fewer options to specify, better documentation. * Several bug fixes. ==================== Basic information about NAMD ====================== Obtaining NAMD -------------- A more complete description of NAMD is available on the NAMD home page: http://www.ks.uiuc.edu/Research/namd/ The software itself is available via anonymous ftp in the directory: ftp://ftp.ks.uiuc.edu/pub/mdscope/namd/ Email questions to namd@ks.uiuc.edu. Features -------- Efficient full electrostatics: NAMD incorporates the Distributed Parallel Multipole Tree Algorithm (DPMTA) developed by the Scientific Computing Group at Duke University to provide full electrostatic interactions in O(N) time. To further reduce the computational cost, DPMTA is integrated using a multiple timestep integration scheme which computes full electrostatic interactions only periodically during the simulation. Scalable parallelism: NAMD has an efficient parallel design that allows large systems to scale well to many processors. The use of a spatial decomposition scheme combined with message-driven execution achieves load balance and the overlap of communication and computation. Modifiable: A major design goal of NAMD is to allow researchers to implement new algorithms and techniques easily. To achieve this, NAMD design and implementation is fully documented in the NAMD Programming Guide. NAMD has an object-oriented design implemented in C++ to provide a high degree of modularity and data abstraction. Portable: For communication, NAMD uses PVM (Parallel Virtual Machine) from Oak Ridge National Laboratory, which has itself been ported to most architectures. Porting NAMD is then simply a matter of having PVM and a reasonable C++ compiler. We have successfully ported NAMD to all of our UNIX machines, which include HP, SGI, Sun, and Linux, both single processor and shared memory multiprocessor. Compatibility with X-PLOR: The input and output files used by NAMD are identical to those used by the program X-PLOR. Thus, simulations can be easily migrated between the two packages, allowing the output of NAMD to be analyzed using X-PLOR or any other tool built for these file formats. Standard MD features: NAMD implements standard molecular dynamics features such as energy minimization, velocity rescaling, spherical boundary conditions, harmonic constraints, and Langevin dynamics. Requirements ------------ * UNIX with C and C++ compilers. * PVM (http://www.epm.ornl.gov/pvm/pvm_home.html). * For generating required PSF structure files, we recommend X-PLOR (http://xplor.csb.yale.edu/xplor-info/xplor-info.html). ========================================================================== Theoretical Biophysics Group NIH Resource for Macromolecular Modeling and Bioinformatics Beckman Institute for Advanced Science and Technology University of Illinois at Urbana-Champaign David Hardy namd@ks.uiuc.edu September 4, 1998 From owner-molec-model@net.bio.net Sat Sep 05 23:00:00 1998 Path: biosci!news.stanford.edu!Cabal.CESspool!bofh.vszbr.cz!news.maxwell.syr.edu!intgwpad.nntp.telstra.net!nsw.nntp.telstra.net!news.tig.com.au!not-for-mail From: "sparky" Newsgroups: bionet.molec-model,comp.lang.modeling.gams,de.rec.modelle,de.rec.modelle.bahn,de.rec.modelle.misc Subject: cool site Date: Mon, 7 Sep 1998 00:09:03 +1000 Organization: The Internet Group Ltd Lines: 13 Message-ID: <6su8ik$gdb$1@toto.tig.com.au> NNTP-Posting-Host: p23-max16.syd.ihug.com.au X-Newsreader: Microsoft Outlook Express 4.72.3110.22 X-MimeOLE: Produced By Microsoft MimeOLE V4.72.3110.22 hi... this is a site where you can go if you stuck with something and you can not find...just ask them...they'll search for it manually and display it for you...even if you like to see a picture or anything..just ask... go to : http://homepages.tig.com.au/~georgeis/sparky/cube.htm. bookmark it and send the address to your friends... it's a diferent site...it's something else.. From owner-molec-model@net.bio.net Sun Sep 06 23:00:00 1998 Path: biosci!reading.ac.uk!l.r.cooper From: l.r.cooper@reading.ac.uk (Lee Cooper) Newsgroups: bionet.molec-model Subject: gromos96 Date: 7 Sep 1998 03:33:39 -0700 Organization: pedal Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: <35F3B56E.A4@rdg.ac.uk> NNTP-Posting-Host: net.bio.net hello m-modellers, anyone out there using gromos96 to model post-translational modifications of proteins? any help is appreciated, answers will be summarized and re-posted. -- regards lee ================================================== \Mr. Lee R. Cooper Bsc(Hons), Dept. of Comp. Sci., \ \University of Reading, Whiteknights PoBox 225, \ \Reading, Berkshire. RG6 6AY (UK) Fax 0118 975 1994\ \Tel +44 (0)118 987 5123 x7645 (0915-1700) \ \Tel +44 (0)118 931 8188 x7817 (1900-2300) \ ================================================== From owner-molec-model@net.bio.net Sun Sep 06 23:00:00 1998 Path: biosci!IRIS.BIO.USTC.EDU.CN!zhouli From: zhouli@IRIS.BIO.USTC.EDU.CN (Zhou Li) Newsgroups: bionet.molec-model Subject: about PLS Date: 7 Sep 1998 06:44:40 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <35F4B53D.237C@iris.bio.ustc.edu.cn> NNTP-Posting-Host: net.bio.net hi,modellers Does anybody know about Partial Least Square algorithms? Need you help. -- Li Zhou Graduate Student on SBDD ( Structure Based Drug Design ) Molecular Modelling Group Laboratory of Structural Biology Dept. of Cell and Molecular Biology College of Life Sciences Univ. of Science and Technology of China Hefei 230027 P.R.China From owner-molec-model@net.bio.net Sun Sep 06 23:00:00 1998 Newsgroups: bionet.biophysics,bionet.molec-model Path: biosci!news.stanford.edu!nntp.cs.ubc.ca!newsfeed.direct.ca!news.idt.net!nntp2.cerf.net!nntp3.cerf.net!news.msi.com!uucp From: "Jeffrey L. Nauss" Subject: Post-doc opening: molecular modeling of protein-DNA complexes X-Nntp-Posting-Host: 146.202.26.50 Content-Type: text/plain; charset=us-ascii Message-ID: <35F42E2F.31A8CDF7@usa.net> Sender: uucp@msi.com Reply-To: jnauss@usa.net Content-Transfer-Encoding: 7bit Organization: Molecular Simulations, Inc. Mime-Version: 1.0 Date: Mon, 7 Sep 1998 19:04:15 GMT X-Mailer: Mozilla 4.05 [en] (Win95; I) X-Disclaimer: _ Unless stated otherwise, everything in this message is personal opinion and nothing in it is an official statement of Molecular Simulations Inc. Lines: 25 Xref: biosci bionet.biophysics:4371 bionet.molec-model:2231 Molecular Simulations Inc. has an *immediate* opening for a post-doctoral scientist to investigate the dynamics of topoisomerase I, its complex with DNA, and possibly new drug candidates. The position will involve extensive use of a variety of software packages being developed here at MSI. This position will be in our San Diego office and will last until April 1999. If you know of anyone interested in this position, please have them forward their resume and CV to me at the address shown below. -- Jeffrey L. Nauss, PhD Phone: (619) 799-5555 Product Specialist, Simulations Fax: (619) 458-0136 Molecular Simulations Inc E-mail: jnauss@msi.com 9685 Scranton Road San Diego, CA 92121 -disclaimer- unless stated otherwise, everything in the above message is personal opinion and nothing in it is an official statement of molecular simulations inc. From owner-molec-model@net.bio.net Tue Sep 08 23:00:00 1998 Path: biosci!news.stanford.edu!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!portc02.blue.aol.com!audrey01.news.aol.com!not-for-mail From: paraleepk@aol.com (Paralee PK) Newsgroups: bionet.molec-model Subject: 3-D structure of DNA Lines: 20 Message-ID: <1998090904080000.AAA18636@ladder01.news.aol.com> NNTP-Posting-Host: ladder01.news.aol.com X-Admin: news@aol.com Date: 9 Sep 1998 04:08:00 GMT Organization: AOL http://www.aol.com I'm taking a molecular biology class. The professor gave us a problem set. He said we could ask anyone for help. I'm asking you! Here's the problem: You want to determine the 3-D structure of a 25 base pair oligonucleotide duplex. The sequence is: 5'TTTTTAAAAATTTTTGGGGGGGGGG3' 3'AAAAATTTTTAAAAACCCCCCCCCC5' A. Describe the kinds of secondary and tertiary DNA structues that youmight expect to find in the DNA. B. Propose a multi-approach experimental strategy for testing your hypotheses. Describe the kinds of information each approach would provide, andhow the approach could be used to confirm or discount your hypotheses. What could be the alternative outcomes and conclusions? I was considering making a flow chart of the approaches and possible outcomes. We've been talking about the various microscopies, NMR and crystallography. I'm assuming we would use those methodologies. Thanks. From owner-molec-model@net.bio.net Tue Sep 08 23:00:00 1998 Path: biosci!agate!newsfeed.berkeley.edu!newsfeed.wli.net!uunet!uunet!in1.uu.net!news7-gui.server.ntli.net!news-feed.ntli.net!not-for-mail From: "David Hemmings" Newsgroups: bionet.molec-model Subject: Re: 3-D structure of DNA Date: 9 Sep 1998 23:32:12 GMT Organization: Virgin Net Usenet Service Lines: 76 Message-ID: <01bddbfe$495dc4a0$LocalHost@dave> References: <1998090904080000.AAA18636@ladder01.news.aol.com> NNTP-Posting-Host: 194.168.60.233 X-Newsreader: Microsoft Internet News 4.70.1155 Well you should really show that you have thought about the problem, before just blindly asking for someone to come up with the solution. Just asking will not help you in the long term. Get a good biochemistry book (Stryer, Voet, Matthews etc.) and a molecular biology book (Freifelder etc.) You have a palindromic sequence, AT rich region (multiple 2 Hbonds) GC rich region (multiple 3 Hbond region) Think about : Random Coil Hbonding (interstrand - obviously) thermodynamically what would GC rich region confer ? measure denaturing temp. intrastrand H-bonding HPhobic interactions (BASE STACKING - nice flat organic molecules with water about them, what will they do ? Cluster -> Helix disrupt Hphobic with methanol, TFA, NaCl measure denaturing temp. measure base stackin (CD, ORD) Cooperative base stacking VdW attractions) amount of 'fraying' at the ends (lack of Hbonding) - what sequenses give more/less ? DNA 'brething' (mportant) - what sequences most likely give it ? motifs -hairpin turns / stem and loop For 25 base pairs i think microscopies really out, only used on V.large sections of DNA - giving a gross structure (strand) NMR - TOCSY, NOESY, INADEQUATE The higher the % of helix, the stronger pattern seen with X-ray diffract. These are just some thoughts, you really should read up on this in the library. HTH DH Paralee PK wrote in article <1998090904080000.AAA18636@ladder01.news.aol.com>... > I'm taking a molecular biology class. The professor gave us a problem set. He > said we could ask anyone for help. I'm asking you! Here's the problem: > You want to determine the 3-D structure of a 25 base pair oligonucleotide > duplex. The sequence is: > 5'TTTTTAAAAATTTTTGGGGGGGGGG3' > 3'AAAAATTTTTAAAAACCCCCCCCCC5' > > A. Describe the kinds of secondary and tertiary DNA structues that youmight > expect to find in the DNA. > B. Propose a multi-approach experimental strategy for testing your hypotheses. > Describe the kinds of information each approach would provide, andhow the > approach could be used to confirm or discount your hypotheses. What could be > the alternative outcomes and conclusions? > > I was considering making a flow chart of the approaches and possible outcomes. > We've been talking about the various microscopies, NMR and crystallography. > I'm assuming we would use those methodologies. > > Thanks. > > From owner-molec-model@net.bio.net Tue Sep 08 23:00:00 1998 Path: biosci!news.stanford.edu!Cabal.CESspool!bofh.vszbr.cz!newsgate.cistron.nl!het.net!news.belnet.be!inf6serv.rug.ac.be!not-for-mail From: Wijnand Schepens Newsgroups: bionet.molec-model,bionet.biophysics,comp.programming,bionet.molbio.proteins,comp.graphics.visualization Subject: visualizing cluster of simple molecules Date: Wed, 09 Sep 1998 16:35:06 +0200 Organization: RUG Lines: 20 Message-ID: <35F69219.6C4B8549@rug.ac.be> NNTP-Posting-Host: lolita.rug.ac.be Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.5b1 [en] (Win95; I) X-Accept-Language: en Xref: biosci bionet.molec-model:2233 bionet.biophysics:4375 bionet.molbio.proteins:13269 Hi, I would like to visualize a cluster (set) of small (identical) molecules. I now the coordinates of every atom in every molecule. What I'm looking for is a simple way to see on the screen how it looks like. - Is there any freeware or shareware program (Windows or UNIX) that does this - What file-formats are appropriate for this goal and simple enough? I've tried to put the coordinates in a pdb-like format, and used rasmol to visualize it, but they expect proteins, and I don't need all that fancy stuff... Can anyone help me along? Wijnand Schepens From owner-molec-model@net.bio.net Wed Sep 09 23:00:00 1998 Path: biosci!news.stanford.edu!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.wli.net!portc04.blue.aol.com!audrey03.news.aol.com!not-for-mail From: paraleepk@aol.com (Paralee PK) Newsgroups: bionet.molec-model Subject: Re: 3-D structure of DNA Lines: 19 Message-ID: <1998091012044700.IAA07713@ladder03.news.aol.com> NNTP-Posting-Host: ladder03.news.aol.com X-Admin: news@aol.com Date: 10 Sep 1998 12:04:47 GMT Organization: AOL http://www.aol.com References: <01bddbfe$495dc4a0$LocalHost@dave> Yes, you are right. I need to work out all the possible structures and then develop an approach to select the correct one. I'm sorry I didn't proffer any ideas earlier. I was just trying to get it out there and get a response-- didn't know how long it would take to get help.-- while I was working on it myself. Yes, we are supposed to research it out in the lit and texts. Why do you think NMR/2-DNOE is inadequate? I've had another response that NMR should do it all. My teacher seems to lead us to 2-D NOE. I was going to work out the possible structures, then use a selective enzyme for single stranded DNA to confirm that there were loops running a gel to see if it was broken and if so into how many pieces to narrow down the options before moving to the next thing. Thanks for your help. May I continue to ask you questions as I work through this? Paralee From owner-molec-model@net.bio.net Thu Sep 10 23:00:00 1998 Path: biosci!agate!newsfeed.berkeley.edu!sunqbc.risq.qc.ca!news-peer.gip.net!news.gsl.net!gip.net!portc01.blue.aol.com!audrey01.news.aol.com!not-for-mail From: paraleepk@aol.com (Paralee PK) Newsgroups: bionet.molec-model Subject: Re: 3-D structure of DNA Lines: 35 Message-ID: <1998091100394100.UAA26697@ladder01.news.aol.com> NNTP-Posting-Host: ladder01.news.aol.com X-Admin: news@aol.com Date: 11 Sep 1998 00:39:41 GMT Organization: AOL http://www.aol.com References: <1998091012044700.IAA07713@ladder03.news.aol.com> I did kind of wonder about INADEQUATE. Thanks for clearing that up. Alot of the methods you suggest are not in the general texts. We have been referred to Glasel's - Introduction to Biophysical Methods . . . but it isn't available at the moment (I'm bugging the prof to get it on reserve). Thank you for your advice. I did some reading today. Your clues provided me with some direction. I think I'll have some hypotheses by tommorow as far as the specific probable structures. Right now I'm pretty sure it: -isn't circular: need to have 80 bp minimum to circularize -isn't Z-DNA in any portion: need alternatine purine pyrimidine and supercoiling that can only occur in circular DNA -could be 3x helical, but that requires supercoiling/circularization which I'm assuming we don't have . . . the "breathing and fraying" of the A*T region wouldn't provide enough energy to make this happen would it? -could be bent 8.7 degrees for each dinucleotide, best seen if a long repeating strip were synthesized -could be slipping- my teacher said left slipping (because of backbone config.) is preffered, does that mean the 5' end slips to the left/ -is not forming cruciform: supercoiling required for energy needed, hairpin has to have 3-4 unpaired bases in it, 10 base pairs minimum are required Do these conclusions seem right? I based them mostly on what I read in Sinden's book-DNA Structure and Function. I'm tending to think of using: NMR-NOE Tm-Absorbance At first I wanted to use an enzymatic digest to select for single stranded DNA, but I don't think there is going to be much. I need to find out more about NMR-NOE. Glasel's book seems to be unavailable here. Any other resources? Thank you for your listening ear! Paralee From owner-molec-model@net.bio.net Thu Sep 10 23:00:00 1998 Path: biosci!biosci!not-for-mail From: "clive delmonte" Newsgroups: bionet.biophysics,bionet.molec-model,bionet.xtallography Subject: Triple-stranded DNA Structure Date: 11 Sep 1998 08:36:41 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 43 Sender: daemon@net.bio.net Distribution: world Message-ID: <35f928ae.0@news.netdirect.net.uk> NNTP-Posting-Host: net.bio.net Xref: biosci bionet.biophysics:4389 bionet.molec-model:2240 bionet.xtallography:4392 The formation and properties of triple-stranded DNA are now described in a substantial body of literature. However, I have been searching for early research reports identifying triple-stranded DNA and I have come upon a problem. I would be grateful to hear what thoughts others have had about it. Leslie et al.(J Mol Biol 143 (1980) 49-72) reported that a fibre of poly(dI).poly(dC) which gave sharp X-ray diffraction spots shortly after being drawn was unstable and transformed irreversibly after a few days into poly(dC).poly(dI).poly(dC+). This new molecule also gave sharp spots in its X-ray diffraction pattern, and this new pattern did not have any of the original spots. Therefore the conversion was complete, or substantially complete. Very similar results were reported for fibres of poly(dA).poly(dT) converting to poly(dT).poly(dA).poly(dT) (J Mol Biol 88 (1974) 509-521) and for the formation of poly(U).poly(A).poly(U) (Nature New Biology 244 (1973) 99-101). The problem is evident: How and where would the torque arise inside a solid fibre that would permit one double helix to rotate so as to unwind one strand and rewind it onto an adjacent triple helix having a different diameter and rotating at a different angular velocity ? Inside the fibre, individual molecules would have a random axial translation so only very rarely would two adjacent double helices have the same axial starting point. How would there be a very high conversion of double to triple stranded molecules when any rotation of adjacent double helices would normally find the free ends of one of them axially displaced down the fibre compared to its neighbour ? None of the original workers offered any explanation as to how this conversion might be possible, nor, indeed, remarked that there might be something to explain. Naturally I have given this problem some thought, but I have been driven towards a daunting conclusion. I shall be glad to share my thoughts with anyone who lets me know they are interested, or anyone who offers their thoughts on this problem. clived@ndirect.co.uk From owner-molec-model@net.bio.net Thu Sep 10 23:00:00 1998 Path: biosci!agate!newsfeed.berkeley.edu!news.maxwell.syr.edu!rill.news.pipex.net!pipex!server1.netnews.ja.net!hgmp.mrc.ac.uk!csansom From: csansom@hgmp.mrc.ac.uk (Dr C Sansom) Newsgroups: bionet.molec-model Subject: Virtual Reality in drug design Date: 11 Sep 1998 11:43:14 GMT Organization: UK HGMP Resource Centre Lines: 45 Message-ID: <6tb2ci$9ha$1@niobium.hgmp.mrc.ac.uk> NNTP-Posting-Host: iron.hgmp.mrc.ac.uk Hello, Firstly, I would like to thank everyone who replied to my post about articles for Nature Biotechnology. The first article, on molecular modelling, provisionally titled "Extending the boundaries of molecular modeling", should be published in the October issue. I'll get back to those people who specifically wanted to talk about bioinformatics when I know when the editors will be needing that article. That is not all, tho': I have another request. I edit a regular feature in "The Biochemist" (the magazine of the UK's Biochemical Society, which goes out free to all 8000-odd members). This is called "The Cyber- biochemist": each article features a new or interesting development in computing which we hope will interest non-specialist biochemists. I may either write or commission articles. I am intending to feature the uses of Virtual Reality in drug discovery (current or potential) for the next issue, which has a deadline of the end of this month. I would be very interested in any potential contributions. I would be happy to talk by phone, but you may prefer just to send me your thoughts, sample Web pages or reviews by email. Alternatively - if anyone would like to *write* a short piece on this subject for the Biochemist, it's not too late.... The "rules" of the Cyberbiochemist are very simple. There is a fairly strict word limit of up to 700-800 words (not including space for references, urls and/or one diagram). Articles are to be written in an informal style and aimed at non-specialists. As this article will appear in the Christmas issue, the style will be even more informal than usual ;) Thank you very much for offers of help, in any format ;) I must apologise for the short notice. Please reply by email, preferably to the address below. Best regards, Dr Clare Sansom c.sansom@mail.cryst.bbk.ac.uk Honorary Teaching Fellow, Birkbeck College, London, UK Freelance consultant and science writer From owner-molec-model@net.bio.net Thu Sep 10 23:00:00 1998 Path: biosci!agate!newsfeed.berkeley.edu!oleane!jussieu.fr!moka.ccr.jussieu.fr!not-for-mail From: ptitjean@ccr.jussieu.fr (Michel PETITJEAN) Newsgroups: bionet.molec-model Subject: sequence alignment poorer than 3D alignment Date: 11 Sep 1998 12:02:31 +0200 Organization: CCR - Universites Paris VI/VII - Paris - France Lines: 6 Message-ID: <6tasfn$3sf4@moka.ccr.jussieu.fr> NNTP-Posting-Host: moka.ccr.jussieu.fr Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit Is there some pair of proteins having a sequence homology (% similarity) significantly lower than their % 3D-similarity ? (any definition of these percentages should work). References are welcome. Thanks. Michel Petitjean, Email: petitjean@itodys.jussieu.fr ITODYS (CNRS, ESA 7086) ptitjean@ccr.jussieu.fr From owner-molec-model@net.bio.net Sat Sep 12 23:00:00 1998 Path: biosci!pravda.ucr.edu!awabi.library.ucla.edu!128.230.129.106!news.maxwell.syr.edu!nntp.news.xara.net!xara.net!server5.netnews.ja.net!HEAnet!web3.tcd.ie!acer.gen.tcd.ie!tpwalsh From: tpwalsh@acer.gen.tcd.ie (Thomas Walsh) Newsgroups: bionet.molec-model,bionet.biophysics,comp.programming,bionet.molbio.proteins,comp.graphics.visualization Subject: Re: visualizing cluster of simple molecules Date: 9 Sep 1998 17:28:42 GMT Organization: Urmhumhan Lines: 13 Message-ID: <6t6dsa$1pg@web3.tcd.ie> References: <35F69219.6C4B8549@rug.ac.be> NNTP-Posting-Host: acer.gen.tcd.ie Xref: biosci bionet.molec-model:2245 bionet.biophysics:4393 bionet.molbio.proteins:13292 Wijnand Schepens wrote: >I would like to visualize a cluster (set) of small (identical) >molecules. >I now the coordinates of every atom in every molecule. What I'm looking >for is a simple way to see on the screen how it looks like. > >Can anyone help me along? I haven't used it but PREPI might do what you want: http://bonsai.lif.icnet.uk/people/suhail/prepi.html Tom Walsh From owner-molec-model@net.bio.net Sun Sep 13 23:00:00 1998 Path: biosci!biosci!not-for-mail From: "clive delmonte" Newsgroups: bionet.biophysics,bionet.molec-model,bionet.xtallography Subject: DNA Structure Discussion Continued Date: 14 Sep 1998 10:56:30 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1007 Sender: daemon@net.bio.net Distribution: world Message-ID: <35fd48d9.0@news.netdirect.net.uk> NNTP-Posting-Host: net.bio.net Xref: biosci bionet.biophysics:4396 bionet.molec-model:2249 bionet.xtallography:4399 Bill further asks whether the side-by-side structure set out in my first attachment does not contradict the structures derived from the fibres. This is a key question and requires a full answer (if you'll indulge me a little.) There are several aspects to this. First, Crick had never seen any of Franklin's (high quality) DNA fibre diffraction patterns when he and Watson proposed their structure. Watson had seen a B-DNA pattern only very briefly and could not study it at leisure. Plectonaemic winding in DNA had already been proposed by Pauling & Corey early in 1953 and Watson & Crick seem to have adopted that idea. A R Stokes, a colleague and co-worker of Crick, had deduced an important geometric ratio for the classical helical cross (Prog. Biophys. & Biophys. Chem. 5 (1955) 140-167, page 163) (see attachment) such that, for the angle from the vertical (delta), tan delta = helical pitch / (helical circumference). In Franklin's famous B-DNA diffraction pattern (see attachment), delta = 40 degrees approximately, and, as the pitch is known to be 33.4 Angstroms from the layer lines, we deduce that the major diffractors lie on a helix of diameter of about 12 to 13 Angstroms. This is close to Franklin's deduction of a diameter of 11 Angstroms from her Pattersons. I now have seventeen published DNA fibre diffraction studies in which the researchers make it explicitly clear that they chose a unit cell which would be large enough to accommodate a double helical diameter of some 19-23 Angstroms even though their patterns of systematic absences in the reflections would have allowed them to choose a smaller unit cell (into which the double helix could not be fitted.) I also have references to unpublished studies in 3 or 4 PhD theses where a larger unit cell was chosen than the raw diffraction data actually required. I should be very glad to forward the list of references to anyone interested. If you choose the smaller unit cell allowed by the dffraction pattern and recalculate the helical diameter (a simple geometric exercise, of course), you get diameters in the range 11.2 to 13.6 Angstroms. A really fascinating study in conventional chemistry is that of James & Mazia (Biochim.Biophys.Acta 10 (1953) 367-370). They discovered that 1 mg of dry calf thymus DNA could be spread in a monolayer to cover 0.28 square metres. They didn't notice that from just this result alone we can directly estimate the minor axis diameter of the duplex as 11.7 Angstroms. I would be very glad if people would like to calculate the diameter for themselves and compare their sums with mine. (James & Mazia measured the major axis diameter, that is the height of the spread film in a separate experiment as 21.6 Angstroms. Therefore their results lead us to a duplex with an elliptical section.) The new model for DNA structure leads to the presence of a "front" and a "rear" face with great implications for protein-DNA recognition, including the way such DNA would wind onto nucleosomal cores, for example. What problems would people see with this account so far, please ? Perhaps from NMR or diffraction from true crystals of oligonucleotides ? clive delmonte clived@ndirect.co.uk begin 666 Pagis Document (4).xif M24DJ`%@%``!815)/6"!$249&`@`$``(`, ````$`1 ````,`S (```0````` M````#P!#;&EV92!$96QM;VYT90`````!`( ``0#0`@`````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````!```` M`0``6 4````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M````````````````````````$ #^``0``0```````````00``0```$42```! M`00``0```-D+```#`0,``0````0````&`0,``0`````````1`00`' ```!X& M```6`0,``0```&T````7`00`' ```(X&```:`04``0```/X&```;`04``0`` M``8'```>`04``0````X'```?`04``0```!8'```H`0,``0````(````Q`0(` M& ```!X'``!*`00``0````````"JAP0``@```#*F````````-@<``%\'``#7 M!P``F@T``#H6```Y'P``62<``/$O``#F-P``"3X``*M#``!Z2 ``;4T```)2 M```W5@``9%L``)I@``!L9P``WFX``$9X``!5@ ``^H,``'.&``!1B ``PX@` M`/2(```NB0``>XD``"D```!X````PP4``* (``#_" ``( @``)@(``#U!P`` M(P8``*(%``#/! ``\P0``)4$```U! ``+04``#8%``#2!@``<@<``&@)```/ M" ``I0,``'D"``#>`0``<@```#$````Z````30```#<```"0`0```0```) ! M```!`````````) !````````D $``'8Q+C @6&5R;W@@26UA9V4@1F]R;6%T M`/________D!]K&BA/!$=_Q#WN&5:B/Y`=,J@B.J'K?A]1_____\`$ $____ M____("X+5W6^M&Z-[]]:C__Y`5UZ=;>BD^@RW+:TJQ[_:*DVM9D+JSIQ4(.@ MG("JHU_Z2;7Z.Q"2UZP^U^G5U&%DRE7]JE:6%=UU'K^VM7"W;7PZ0X[ZD!8K M@Z3R`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`0 0FX8:A6(B#*9%8\14R-LS(G,V9& P?#!,$= -$8C-I> M\$1T@8,&M-_7UJE]'UZ_3_O$8=Z6G^^E"5=4'>1^2G#/5"A=+M@VGZ[W6QZI M+JTZ":=A_?]OZ]+ZF_Q(S7U7PG#3#_""T$GM:;7NYS":OA:VFKOX1QX0?ZJ'Q07<)!*L8=VLZIA]A)]83O7OO3KU MZTO\4PN@L2!33%2:7_^B,'__3_N[X00JD0.VG7*+&01WT"IU0=+TDM?^P MOL;5UZ;DW]_>2/_KNT'J$)*=!)A;2[M]!-K:WZ:36@2Z5Z)HGSLOK_%6NU[W M[O:;BJ72V$&@F3[PB::IJG9TJ;M7A),>KL/]=/2L(,+7T^OO?7JEO_]O[X41 M_K]!)H.ZX:O23:A%CX9G])]L*7?"7O':JD^^UZ';"=/A5[)$ZB%W2_#:$)!! M`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`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`("R*9G1DXRB+9HB09&B(9$K1 M.BH1:!H9#"%6CH"'4C1FL'(AGLD"G04@8<[&QD@B5D=^.E^GJXCW::?2]_UK M6@_EL%#5[Q[5/%!*T^P1VC^%_\M@/6K?^PG^/9%-?22U]P[""#^0C?:D+=4E M!R=EQBGK"J3TD_29#KPWW6L)W^T&&:210G7;+158:%6W,#5;L+1 M`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`D=JQR%':Z:7U3%5]J*7B1=AK] B!>CT@2;IH'M-)K\GP__J MM?K<$$T.[D2:3";<(,\T$-4DF#6FG5UJK7Q45K3(EUO:#AK6MA_O[5=KB"6T M=H1'3;<-?T0OEV,)#7;I)_VE1QI#PETA]IK7XOEI`JS1>]P;]/I=<$0+W+4' MS_M6]!DK\5UN+22#9C_@`@ @FHRR%9&M% MIBH<^9D!!2-&5899F(0Q",(@2(B+36@I\S@I.*= Y\RG(U@TDXSNM&HRG(@P MA)2(4/@ZA]A^G5 HAJ@VO_8I*[YV,/MY:=D*J9K9, M4+I7R%RUIOQ\D?^KUW^J>-PK)'NO[!BZ<[,SU604<\/"%D.X4:PK?3Q4(.G_ MT60R%)&U^O"]AZ0:];2R'?W7M5L'7SLE:?:K#3;:H;1"PTK:5/=-?Q^]CH?K M5IK7I/AO^OM??AL*GI-Y/4R"0-1]**##'5V]:Q^X7?=-/H-^AN^K3_6O%;.Q M*M8]!!IL)+((/6W/!!=K^_JM_NU=:"?[_^ONER+D+2=4&0EM!=/(-5S/])X3 M+!R*OOOA.K2OWINEJ_"?M/M/?\/54&@3TZPTWJK82&Z(RJNA?JN3<+MSL_XF MH#&O^U\:JGUK=T0+O@F&BT@(PT525:N&$%5!K&N@F@EV%=$Q-XA-AOCD,VOK:]A! MN&T1>B$K&]W_(32U%_[O'<-?]?_7O:_]-NQ3D#-7A[WP[3_'[]?]K#?_^U?\ M@[G(@T*3Y&)KM;+52$D\AJ3KJKI>15'KT'4[X:W^]^D4E?780/!'G5,()^&V M+)W5FYGCMJOA,*^=G7QI?&W2_[4?O_U?3[0["J&Z3ZM1OMJKI:7"7^T4A7TG MO]&2=4+A,5E/SYTAW1"#WR=C0J=U,)>[:'?TU?I M+00?]M?\&>#06D$9F8_A-.H55R"E@A>H6E.\>OCM7[7J%5+356GVO_27+<>^ MP1!!X1!Z82]/7:API&VF%HU_]5^*#=5"5:FB]IK^FEJ55$)]VLR4DOP3P5!) M(+B&G-%H>H5"M0FG\LTTFL)KZ5UZ2[6G[JK6M_L)C#3^U=!2.(2PN_H-7*.$ MH-.@D_^\,I;^.U2;2UQ_6TZ:#6NU\LT%E8<'#"]=4V= B1UTUVT)#2,522K5 M!#]=R4I./^O"T$__77__+\XY;ESDL&FEH&OV@PE3PEM.07'MQ(M]9?!0OH(+ MT(CH/]>OM>U_I_?\?]>(:1!=F*:753]X=#JT$TL,)KM.Z540)=.@N,AL9A;7 M[OV09;_[6\?6O"J_#7X=881J:J$ZVG"2V$$:C%J[;]\2!8KB*"_KITFO_Z'= M6O_A;?VH8)(4]09!Q3=,[I!0L9#*ZGI=A=IL[5MKT*^TW7Q2R%[U#[WOZUKL M@[,,OAH-Z]*@A6GD,JW:\:1JAM,=06GNV_ZO^&KUW23_ADV;83;AA.ZK2U5, M*G-6QK"K[(KD_QUP?#ZZK5/VO?_W>R;@0B"=MZ=;6W06+3#3?@JRDH94-TPO M)NK#'K[Z?U7WWXU=VMA=`@;5/3V$XD-$-/JJM?06MJ5;V*Z#Z[SM6U[2A:(:; M^M:5+]0FD:_NOTB!`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`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`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`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`! ! M)J%)OJRY&8,'? Y:!@'@IG@/`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`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`QKL8,.+>3'(MH+^GI^.$[?N'M:H0ONG#7_Z MJUVVO]D&DU37O"KU9%R+=I:(W8FK)3'!"'A-B&"%_?WW:;=-*$&U%T+B^''2 MM6AAPT+WJ&J\/TZO?ZKK>@RL(8BG"C>&V$T$*"D'C[$-,OSCU7;^US119SOR M0'+0'P:('AIX%<-D% QJD:I4'$.N])QUPX;JDJKKVAD$FGD&^=M Z05[#M?1 MV!Y%3TJ2;NZ#7;=YU61GQL('=/N@974(T[A,O%"VPVV$#"(;.FC"#]IMKZW! MIO5;A'A^PX<)X_H..ET\*W6T()!O>&A3A!_O5=DGQ_5$#U?W5^ "`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`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`AAI^J%U=D"'[:0]#6E87[C3XI2&5855V$$L2!H&UW[_I4_C04IVUYZW0UT2 M>$"QPX:2ZIN_CX54EK_JP@_05*^K$:]6/OZ[_W7Q,D^$UV%DQIE"@=K_X/KU^G8[_R!F#4 M^&60D&O7H/#^@_]!]#/[UI?U:#X:D+!"9K7]IT$N+40?J"$'_37$4E^'QF&M M/3@J)1"WO(&@*^/U^T&2@<+_]^(U(*.<(0UL@[M^DJ'_YXW6$Z]?_;P@]*G8 M37Y(OK:@@?7(@[=^N_?R>!R[K4(./2_TUOZM+W__H-5IZZVG+<:>.R"787CV MOO^^[\>$&%SH$X?T^GH)^60+XD-EQKNE]=/?V60H82;@GAC[JC1A_ ! !":A M@FY6CO&606,\!H&4>,B1ED6\X#060PR3U#WL$SG45_2[KMSLK1HE M>H8W7KL$'(9 ;(NWG8&D&FTY9$:K3_JKID,D,[UZ35=4^Y T`L>_H)*=C@T4 MT/NFFD3<"1VE`TU=4:W(-QPA'_E;@O1Y+NL:#_J[00?VM1(9C)Z[7:AA=W^D M&D_L)<+XUI#]+WM)^Q2L@79-/^OM-8QLP_ZPOU_^GIH/PUUK:=!4%=#N(?M+ M2:W^D0+]W^&$L@NR"&FG^$@GBO\/\6JJGDZZ_X,I.E8M>PF$SX.W_Q!@N0R MMO5=-+_XR<&M88^'8086KO]#==\1W_Z&I#)!FOPUR&Z7_[($1_/+^0MO[1K: MPF@V^@P3O'#?Q_<:V/_OP[WK7=IECK\AV3N^M\)IH-_1`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`:H5IVG"5/BE^+:A+A/3Z_^TG[M0EUSLU"EV"M>F@JNZIIXJ MOZ%A)]-:I?KT1OX`( (FH,$V,Q2<-4F0:1-UH,@,LH#(!2.!EFLBSJH*A7(! M"O0%DM VSKD3&0,S@&@0U/W_?OK8N0YK^UJ\7T$].DU#0_U]KOTM:_\AD*\- MIJ[ZM0_?TG08ZUI?^%(GED9#O)N+7^G;6NUQE@6F0D4UPB!Y/[ZW7]-21.WP MZ9 \%%]7KUKM>';7"5?V.DOQ&%MG`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`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`@V&JVK7ZT'\6K%I MKO^L)A!X<(-B/']?[:_^=C 17VZ/L-R>&ROPOW#\M)2V'C_P7[#CA!MA)AH@ M>33)?"UVP_;J/RS$+],,-6WPO3?Z4,>Z_0:.[#,M-6Z??B*??>VPD0K^"IH4 MRAX>Q809`S L:9GA$,N\,*(>@Y:1CWX1`N;C8D,@-:X:&PG31%GPM./U_!!! MIL-I[WU)N4@9AF?[KRR"X%RYM>OONFMOO_1-U/(*&4[)QO3JZVH2Z>VT_])H M$&4;EMVS4GEX^&']PE[5.Z?AUR;A`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`URS`L4NCPI1$<,D&A;OK_(-X99Q!G@T$,9MEP+ET$(B-+^$N)!JAPFR MT! -`S&2;+(<#8%62=V%\*MO)N) MY'1(`V!I*UO?I:AA,FQ2CIF@4N!M:":>_T1?ZO+#8E"VO_6T5SH^*4`2D'2K?UIU;G:RR^>R.R.'(X$ MHC8:H9 N.4YX""0R!H#>X\FYK:JKPHB(B((IT3Q'Y#14DL?!X5BL40-H*XY MO!AS[* B5AII?3LD:[<@; )[).0[E#G'*0=05M*@TE7J3@0RXOL-$#8&XY!T M%+@;:4M);?ZHG!WY#(!I>#P0B]5\`$ $)J&PFQH9V89+2)NG#(#,.!DAH*@9 MD<#0&DOD<(1T1S,\UHTBW, 02T,LEH4M(S,J`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`:$Z:AWX29 OP>_TT'_!H?3[Z=5Z^^Z^*^K_""5XU3'!U[]=ZIUU[_1_ MV_U[#AA*JM6\.#2#__D#7Q7_[C]WZT_A3.@TG^'2_^[?TD[2[[_#_W3]#0W0 M=7""[?]?U2=!UZY-] AT10]UQO]<5(:X7#0A^U3^%_A/^&AVUWV$U<*DW'_: M_UIZ6WIKC[J$TO^0?L?_K_O3_('@H<-=K?ZN_W%K]5Z7_Y S!4Q_H)IU+,FT M%#6OU?]?_]=H--A?H,)=-!-J/E&Y;[(JO2K[5N0R=_$'C0-WH=I50800;_JN MOPTF/[^ZI?%=\FXV/7N\[*/_^$0=EF[]0J82;#V$'PG:]WU6\7P1`OLNFZ_A!A$'-Q5#>"I+OM],K%^N%[K^I5 B#_"A, M(*AW]H-?PL/OKJ@X?KG9Z#7^`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`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`/#*\+#08D,D&O C2UQ5?VPD&/T_3J__K=TY1=Q]A>%76@6K"3"7 M]-=O_^L?]SLI1'%?JM?4:Y#-?(9 G()?!"W_O___KV0-!$-!NJ]H-:]*Z"9! MT0/6QD_ZT^*"]JO]NL$M]?\ $`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`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`(":@T$W$&=_$8(648"V!6?#(SJ1[*J#D4!0>#*(M MD,0D@91:JT,T!RD(ZQ,(E+/@N:C)<9@#!H!H"KWVM.[JG3U02^+KH:(&89W7 M^H<@>#0^PEI/82""Z05M(MP2:Q^]V(N.M["2^N,0C/6U[>TR&7?-1'K2>*03 M"U.=A%XD\+6\J[VG]=+U+43OZ3U82Z:^&%\J,-.IP@A_P@@K'2W)/00I*>@A M71#Z?O=4*/=UPJ7A+=A?"'I#O4&N,)JE80023.Q'#T$#!K6AD1]7Z=Y#/"[6 MB!@W2?M+=U2U_:85]$$0G_"257:5/2^%WZA-KIK_206L/7'K2UQ($6MM*AM+ M(<%2:6&$DG5A:7XW^7:;7TDNK]<=?[ZP@UKPPH00]TXIVO_AA>$VNF%2H-[= M=;D#$=N*$--]`Q)6DD@VUG=I.1P0SPZ\DH_BVNTR>5I^TUJNO!$:_5M4J#"# M2"';AIO0:9 T!GPN+7K[[7:%K+52\D5KD"L'^0>$T]<1^V&FK0@@D':$/L_; MTTT&A#C]O;6H(C]:GU_K7U_D,SBQS_#[#0="*2>FB#WU3=Z?W\A"A^V@U))I M;].MJ_6O^3!(:N+6P@@ETC8%)^G=+2(B$-U7Z?=NNO5)A5\=6GK?Z2'VNMI5R;JDSST&\,AI0B-82 MU:A![8(*JJO3'7Z#A23TF\WM;]' (V&G\FY0@3;2?80:M-! V%"C"WH/V1P- M`UDN3=6#2QYJZ#TG:3<;\);6GK@L@T]<(,2)`W:54@Q0[#H'V$PU^_?35Z5! M$W06PVGKPDTTW##!-)J$$#4$VVE:["#:7W[VFE57W_]6_J\@3XK3(Q$: M*%H)!,CM4^L)OA'4#P)$O76ZVT_JTXTOM^_U>@]:8AA:"00L(E;)AC2RX7[: M?^I!;!6OZ;3Z7KW5-IKR"-4#Y%\CY:3"?"["T$$A-?#32TGN-AI>$P@__:JP MOA)K=6O:]>L-6K3X6CN!Z""69W;"#TK'#""]/W_&D&"?"X333'25X=?IM##( M'@LWUQP@H35^Z740O3>6?2_O_2&_Z7K_:&T]\*Z2"#"[BD[_;UK^%TD6LLJ* MKZ1 \#ARG7[5-WU$BVXH($PK6D[('@R$?L.PO^FTJZZ]?;.4&$]=/5P02JT2 MK(&7(&+U78MCD._M-26I:"22K>M/3^WH,BCN0-^JM+M($DN$Z__?TTW0;0=J M1N]5+6$[5_7Z]!P^TR!X)-6A#003U:02=!%WKJ0>G2_[0P^(S.MZJEPK(8O_ M__=W9L#"":[%(4E206WX759 \%[WW<[,)I)CK7_5DAUZU__V$&DW5A2"V"C* MIT$$M+!!J'D/UTTTGOAVDQ^OZ"Z54]N$X9(2\7[\082A6J;27A*$"2$6':MP MUM-:?B=V1MI_^K06E_\)K81 \"[O_A"1(A9$L@>'N[JHA)4EA_47)CB$V%1% MQ?C6F@Z^I&JM)+_]IN]_I*TT&MM<(&@M*NNM!$@#P46F$$U]?WX^U_^R'_3M MH;U^X6'_L()PJ"^"7D5W\=#^UI_I-)Z^A6J#:\AD`K.LT1'7)NJM*7U*GZM0 M@@ZH+I7:ZZ::0__#__5)Z_U[7?KY-[&<#+(X=$$'(3$(8I!.]:236)BPZ"NF MU=+&N@T.1?)G_HAFO_2'\?X^]KQP0::TN$_D#$"5G77"5I,H?^.UJGU?:7Z3 M"WI>(=5_XOKKKM(*UJTO;D+>/]7HAMXFZ_=+3]+IZ;_]EH<%T@>[T1C_"3IA M:2TN%876UIU?_J]4A9J>O^W]\I],)A7D-E*HSC6P@N0+H8.E]K$@MA'[D0<@ MQ9!(\/0^U6F_756O[2D#PRXOT2+;/9=&FB!@=-P8(6E?A!52;5:]0F0R`;'" M?T\=J@@?B0/!=ZONDZ7UI?L?V$'AL<(.\(,5#723TZ6T[6OXC__JNNEOWIK_ MIWIW"5P@G2>AU"T[PPMK6]KR!X:F>(::N-O?R,TPEUD'L M<4VD_U3\,)PW]>Z?7O:M:K?M/Q:*L"YUCC8U6$6O3<6UWC3C1#(+"+>KTK"=/_4-Z[>VFO:57 MKR;J`:1#%A?M! W"VR$_PVMQTVD^UC[M=+;N[O6U]:T%#('@5]TXIV%3[V1 M.[PPEQK^GOKK?;0;N'C]K:T'[A=.[7=,)Y#(!:CTE^N0RAZPGK:'=ZM)OW_( M,D0W";1#(!L=^Z8]4PFTF0/$CH@U60/$S]60V6GECOX3:6'N_:^G7#(%CKKO MAJ0QA BU"#=,2#'IM-RI!>F%6:P+K3:U(X"#ANH`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`\$.`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`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`( ()J&J4T)Q5LN*=\1+D6J&S-D01+@U%(%)`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`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`@":@J$!4S&=FK^@O27?X82")N3?$4^O3 MY 6"F_("I):7("I4PF%_^3"^MK#A?]-?Z]=VNOL)K>N(]-<@*H=B/3_;_]_] M+M>O[2"X__7^UWVUQ_^O_O______________________[^O_O_Z__^__X (` M(":@K?__________[U]____________________________?_^O_O6_K_[__ M__\`$ $FH,GWK_?U]__7___^___________________O_6]?[___________ M_W_^"Y ?`3H)__7?_UW@`@`@)J"F0'P$Z"?________________]_U__W___ M_____W_____?7\/HVN#R.O%E`/@)&>/_____________________________ M__P`0 0FH,Q ? 2,Q/__________________________________________ M______________C_`! !GAD``&0``````````````````````"@```!D```` M00````$`" ``````9!D``(,]``"#/0````$```````#8V-C8V-C8V-C8V-C8 MV-C8V-C8V-C8V-C8V-C8V-C8V-C8V-C8V-C8V-C8V-C8V-C8V-C8V-C8V-C8 MV-C8V-C8V-C8V-C8V-C8V-C8V-C8V-C8V-C8V-C8V-C8V-C8V-C8V-C8YMC7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U^;8U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?FV-?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7YMC7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U^;:U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?FX-?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7YN+7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U^;FU]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?FZ]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7YN_7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?SU]?7U]?7U]?BU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U^;SU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7\]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?F\]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7Y.W7Y-?7U]?O\]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7YM?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U_/8U]?:U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U^;7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?B[]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?FU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]K7U]?7U]?7U]?7U]?7U][7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7YM?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U_/7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?QU]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U^;7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U][7V.W7\]?S MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?FU]?7U]?7U]?UU_/7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?UU]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7\_'8[>37U^GIU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7YM?7 MU]?7U]?7U^#7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]C7V-C7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]CKV-CBU]?7U_/7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U^;7U]?7U]?7U]?7U]C7U]?7U]?7U]?7U]?7U^+7U^+K\][BV-S7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U^GIXMCOV.W:\^W7Z]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?FU]?7U]?7U]?D[]?8U]?7U]?7U]?7U]?7U^37 MY-CSV-[8V//7Z]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?O\=C8V-C8U]K8 MV.+SU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7YM?7U]?7U]?7U]C7U]?7U]?7 MU]?7U]?7U]?@VO/8V-C8V.GO7U^+8V-C8V-C8VMC8U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?FU]?7U]?7U]?7WMK:U]?7U]?7U]?7U]?8V-C8V-C8V-C8U]?7YM?8U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?DZ=?>V-C8V-C8V-C8V-C7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7YM?7U]?7U]?7U]C8WM?7U]?7U]?7U]?8V-C8V-C8V-C8 MU]?7U]?7U]C7U]?7U]?7U]?7U]?7U]?7U]?7U]?7Z>3@U_'SV-C8V-C8V-C8 MWM?7U]?7U]?7U]?7U]?7U]?7U]?7U^;7U]?7U]?7U]?FU_/7U]?7U]?7U]?7 MV-C8V-COV-C8V-?7U]?7U]?7V-?UU]?7U]?7U]?7U]?7U^O7U]?7U]?7U]?7 M\=C8V-C8V-C8V-C7U]?7U]?7U]?7U]?7U]?7U]?7U]?FU]?7U]?7U]?7[]?7 MU]?7U]?7U]?7V-C8V-C8V-C8[]?7U^_7U]?7U]?SYM?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?MV-C8V-C8V-C>[]?7U]?7U]?7U]?7U]?7U]?7U]?7YM?7 MU]?7U]?7U_77U]?7U]?7U]?7U]C8V-C8V-CDYM?7U]?7U]?7U]?7U^37V.W7 MU]?7U]?7U]C7U]C7U]?7U]?7U]?7U]?8V-C8V-C8V-C7U]?7U]?7U]?7U]?7 MU]?7U]?7U^;7U]?7U]?7U]?OU]?7U]?7U]?7U]?8V-C8V-C8V-?7U]?8U]?7 MU]?7U]?7U]C7U]?7U]?7U]?8U]?OU]?7U]?7U]?7U^O>V-C8V-C8V-C8U]?7 MU]?7U]?7U]?7U]?7U]?7U]?FU]?7U]?7U]?M[]?7U]?7U]?7U]?@V-C8V-C8 MV-C7U]?7U]?7U]?7U]?7U]?8VM?UU]C7U]?7V-?7U]?7U]?7U]?7U]?7YM[8 MV-C8V-C8V._7U]?7U]?7U]?7U]?7U]?7U]?7YM?7U]?7U]?7U]?7\]?7U]?7 MU]?7V-C8V-C8V-C8U]?7U]?7U]?7U]?7U]?7X-?8V-C8V.+7U]K7U]?7U]?7 MU]?7U]?7U]?BV-C8V-C8V-CKU]?7U]?7U]?7U]?7U]?7U]?7U^;7U]?7U]?7 MU][7U]?7U]?7U]?7U^O8V-C8V-C8W-?7U]?7U]?7U]?7U]?7U^G7U]C7V-?7 MU]?8U]?7U]?7U]?7U]?7U]?7\]C8V-C8V-C8\]?7U]?7U]?7U]?7U]?7U]?7 MU]?FU]?7U]?7U]?8U_'7U]?7U]?7U]?8V-C8V-C8V-K7U]?7U]?7U]?7U]?7 MU]?7U]?7V-COU]?7U]?7U]?7U]?7U]?7U]?7U]?8V-C8V-C8V-?7U]?7U]?7 MU]?7U]?7U]?7U]?7YM?7U]?7U]?7[]?8U]?7U]?7U]?7V-C8V-C8V.;7U]?7 MU]?7U]?7U]?7U]?7[>WDV-C8U]?7U]?7U]?7U]?7U]?7U]?7U]?7V-C8V-C8 MV-C7U]?7U]?7U]?7U]?7U]?7U]?7U^;7U]?7U]?7U^_7U]?7U]?7U]?7U]C8 MV-C8V-C8U]?7U]?7U]?7U]?7U]?7U]?7U^W7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7XMC8V-C8V-C8U]?7U]?7U]?7U]?7U]?7U]?7U]?FU]?7U]?7U]?IU]?7 MU]?7U]?7U]?8V-C8V-C8V-K7U]?7U]?7U]?7U]?7U]?FU]?7U]?@U]?7Z]?7 MU]?7U]?7U]?7U]?7U]C8V-C8V-C8V-?7U]?7U]?7U]?7U]?7U]?7U]?7YM?7 MU]?7U]?7U]?MU]?7U]?7U]?7V-C8V-C8V-C8U]?7U]?7U]?7U]?7U]?7Y-?7 MU]?7U]?7U]C7U]?7U]?7U]?7U]?7U^;KY-C8V-C8V-C7U]?7U]?7U]?7U]?7 MU]?7U]?7U^;7U]?7U]?7U]?7Z=?7U]?7U]?7U]C8V.#8V-C8V-?7U]?7U]?7 MU]?7U]?7U]C7U]?7V-?7U]?8U]?7U]?7U]?7U]?7U]?7V-C8V-C8V-C8U]?7 MU]?7U]?7U]?7U]?7U]?7U]?FU]?7U]?7U]?IU^;7U]?7U]?7U]?7W-C8V-C8 MV-C7U]?7U]?7U]?7U]?7U]?>U]?7U]?7U]?7V-?7U]?7U]?7U]?7U]?8[]C8 MV-C8V-C8U]?7U]?7U]?7U]?7U]?7U]?7U]?7YM?7U]?7U]?7U]?IU]?7U]?7 MU]?7U]C8V-C8V-C8WM?7U]?7U]?7U]?7U^_7V-?7U]?7U]?7U^+7\^W7U]?7 MU]?7U]?7V-[8V-C8V-C8V-?7U]?7U]?7U]?7U]?7U]?7U]?7U^;7U]?7U]?7 MU]?7VM?7U]?7U]?7U]?8V-C8V-C8V-CFU]?7U]?7U]?7U^_7U]?7U]?7U]?7 MU]?7U]C7U]?7U]?7U]?7U^;8V-C8V-C8V.W7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?FU]?7U]?7U]?7U][7U]?7U]?7U]?7V-C8V-C8V-K8[]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U_/7U]K8V-C8V-C8V-C7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7YM?7U]?7U]?7U]?K\]?7U]?7U]?7U]?8V-C8V-C8V.W: MX-?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?8Y.G7V-K8V-C8V-C8V-CK MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U^;7U]?7U]?7U^W7V-?7U]?7U]?7U]?7 MZ=K8V-C8V-C8V-?7U]?SU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]KF[]C8 MV-C8V-C8V-C8U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?FU]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?8V-K8V-C8V.G8YM?7\]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?SYM?8V-C8V-C8V-CBU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7YM?7 MU]?7U]?7Y-?7U]?7U]?7U]?7U]?7U]C8V-C@VMC8V-[:V-?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]K8V-C8V-C8X-CSU]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U^;7U]?7U]?7U]C7U]?7U]?7U]?7U]?7U]?7V-C8V-[FV-C8V.+7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U^W8V-[8Y-KOU][>U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?FU]?7U]?7U]?8U]C7U]?7U]?7U]?7U]?7U]?K MV.W8[]C8]=C7YM?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U_/KV-CBZ>+7Z=?K MXM?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7YM?7U]?7U]?7V-?7U]?7U]?7 MU]?7U]?7U]?7U^37U]?UU_/KVN_KU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?8 MV.+MU]?7XN+7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U^;7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?OU]?7VO7>\]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]K:]=K7U^38U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?MU]?7U]?7U]?7U]C7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U_/:Z=?7 MU]?7U]?7U]?7U]?7U]?7U]KBU]C7U]?SU]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7V-?8U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?UV-?8U]?7U]?7U]?7U]?7U]?7[^;7Y-K7U]?7WM?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]C7Y-?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?SU]KOU]?7U]?7U]?7U]?IV.O7Z]?DU]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?8U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?DU^_MWM?7Z=?7U]?7\^;7 M[^O7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7Z=?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U^;7U_/8 M\=?:XM?7[^37U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7[]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U_77\]CQU^+KU]?7U_/7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?8U][7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7YM?MU^;7Y-?7\=?IU]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7V-?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?BW-?7]=?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]C7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U_/7X-?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]H"``!E```````````````````````H````( ```!4````!``@` M`````* "``"#/0``@ST````!````````V-C8V-C8V-C8V-C8V-C8V-C8V-C8 MV-C8V-C8V-C8V-CFU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U^;7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7YM?7U]?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?FU]?7U]?7U]?7U]?7U]?7U]?7]=?7U]?7U]?7 MU]?7U^;7U]?7U]?7U]?7U]?7U]?7U]?7Y-?7U]?7U]?7U]?7YM?7U]?7U]?7 MU]?8U]?7U]?7U]?MV-CBU]?7U]?7U]?FU]? MU]?7U]?7U]?7[]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7[]?B MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?SXM?7U]?7U]?7U]?7 MU]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7U]?7 =U]?7U]?7U]?7U]?7U]?7U]?7U]?7LHD``%2C```` ` end From owner-molec-model@net.bio.net Sun Sep 13 23:00:00 1998 Path: biosci!biosci!not-for-mail From: "clive delmonte" Newsgroups: bionet.biophysics,bionet.molec-model,bionet.xtallography Subject: The Structure of Duplex DNA Date: 14 Sep 1998 10:54:57 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 75 Sender: daemon@net.bio.net Distribution: world Message-ID: <35fd48dc.0@news.netdirect.net.uk> NNTP-Posting-Host: net.bio.net Xref: biosci bionet.biophysics:4395 bionet.molec-model:2248 bionet.xtallography:4398 Eric, You are right, and it is timely, to raise the topological papers by Crick, Wang & Bauer (J Mol Biol 129 (1979) 449-461), and Bauer, Crick & White (Scientific American 243 (1980) 100-113). Their treatments did not apply to every side-by-side model, however : "..This proves that the linking number is not equal to zero (at least for the great majority of those, i.e., side-by-side, molecules.), and also only to such side-by-side models "...in which the two ...chains do not coil around each other ...but instead lie side by side over most of their length, having only a few helical turns." Their treatment only applies to side-by-side models, like that of Rodley et al., for which there is a net winding because an equal number of right and left turns do not get you back to an equivalent repeat point in space because of the D chirality of asymmetric carbon in all the pentoses. The model I have come upon has exactly and identically zero plectonaemic turns in its relaxed state over any length of duplex and is therefore not covered and constrained by the two papers just cited. Thermodynamics has always been a delight to me, though I find it difficult! Chemical energy is dissipated in all directions; that is, it is not a vector, but torque is a vector. Therefore chemical energy would seem to be unable to either wind or unwind two plectonaemic strands where no breakage of covalent bonds takes place. My solution to this conundrum is to say that neither winding nor unwinding does actually take place ! But tell me - let me tease you a little - what do you make of the results of Kabata et al. (SCIENCE 262 (1993) 1561-1563) who found that RNA polymerase slides along its DNA duplex substrate and does not follow a helical path ? An absolutely crucial test of the new model offers itself, though we need someone to carry it out. Drew & Travers (J Mol Biol 186 (1985) 773-790) have constructed a 169 bp circular, covalently closed length of duplex DNA. With this length of DNA there can only be a very limited number of topoisomers present if the ring closure, of itself, could introduce only a very few superhelical turns. That is, all the 169 bp molecules would share a range of superhelical turns between about -1 and +1. Therefore in suitably mild denaturing conditions, in the absence of any strand breakage, the 169 bp sequence would be largely separable into complementary covalently closed single-stranded DNA circles interlinked at very few places according to the new model. Thus the most direct route to distinguishing between the DNA double helix and the new model is based on topology. The complementary strands in that 169 bp sequence may actually be linked by 0, or perhaps ± 1 superhelical turns due to the algebra of the enzymic closure reaction used on the 169 bp linear duplex, and the denatured covalently closed circular strands could be decorated with a suitable protein and then be examined under AFM to determine their linking number. Therefore, if the 169 bp circular DNA is denatured under mild conditions, perhaps using aqueous urea, it could then be decorated with the single-strand DNA binding protein, the product of gene 32 from phage T4 (Scanning Microscopy 9 (1995) 705-727), so as to be rendered easily visible under AFM, and the actual numbers of crossovers then counted. According to the double helix model, LK will lie in the range 16 to 18, or so, while the new model predicts LK is -1, 0 or +1. A very similar experiment could be carried out with the small circular DNAs described by Bates & Maxwell (EMBO J 8 (1989) 1861-1866), where, for the smallest, the double helix model predicts LK = 9, and the new model predicts LK = -2. Is there any research group with the interest and ability to conduct this experiment ? clive delmonte clived@ndirect.co.uk From owner-molec-model@net.bio.net Sun Sep 13 23:00:00 1998 From: "clive delmonte" Newsgroups: bionet.biophysics,bionet.molec-model,bionet.xtallography Subject: Triple-stranded DNA Discussion on Structure Date: Mon, 14 Sep 1998 14:56:16 +0100 Lines: 1178 X-Newsreader: Microsoft Outlook Express 4.71.1712.3 X-MimeOLE: Produced By Microsoft MimeOLE V4.71.1712.3 NNTP-Posting-Host: 195.7.225.117 Message-ID: <35fd14f7.0@news.netdirect.net.uk> X-Trace: 14 Sep 1998 14:07:03 GMT, 195.7.225.117 Path: biosci!rutgers!rockyd.rockefeller.edu!newsfeed.nyu.edu!btnet-peer!btnet!demeter.clara.net!194.112.50.17.MISMATCH!news.clara.net!peer1.news.dircon.net!peer.news.nildram.co.uk!news-peer.netdirect.net.uk!news.netdirect.net.uk!195.7.225.117 Xref: biosci bionet.biophysics:4394 bionet.molec-model:2247 bionet.xtallography:4397 Let me thank Eric Fairfield, Kandala Chary and Bill Ross for their helpful, initial comments made in response to my request on 11th September for suggestions as to how the problem of triple strand formation inside fibres might be resolved. Kandala suggests that: "In poly(dI).poly(dC) and poly(dA).poly(dT) sequences dI and dA strands have a propencity to accept the third strand on the major groove side. And in poly(dI).poly(dC) and poly(dA).poly(dT) mixtures, before they are drawn into fibres, the duplexes are in dynamic equilibrium with monomeric strands. Thus, when one forms fibers, the need to unwind a duplex is not necessary. There are sufficient monomeric strands which hug to the duplexes, thus forming stable irreversible triplexes. I hope this will answer your question." These are very interesting points but it seems to me that there may be some difficulties with them. The dynamic equilibrium existing between single strands and duplexes could be seen to invite a very similar point about how exactly a duplex unwinds in solution during denaturation. Where does the torque come from ? I have never seen this question addressed. The unwinding situation in solution is very similar in biophysical terms to that found in fibres during triple strand formation when one duplex also has to "unwind", it seems to me. Moreover, in solution, the duplex is folded into 3 dimensions, compared to being linear in fibres, and unwinding might be even harder than in fibres. As I recall, the major groove does not exist on one side of a duplex, but follows a helical path around the duplex axis. If a third strand is to join the first two by lying in the major groove, it would have to be wound onto the original duplex, I believe. The initial fibre X-ray diffraction patterns were characteristic of those ascribed to duplexes and distinct from single strand diffraction patterns (from poly(rC) and from poly(rA), for example). Therefore single strands would seem to have been absent initially, but were produced in forming the triplex plus a residual single strand over a period of some days after the young fibre had been drawn. Bill suggests that: "Not knowing much about fiber diffraction, it's easy enough to speculate that somehow the original duplex was somehow not really a duplex, but assuming it was, I wonder if you are thinking about bond breaking, maybe some sort of autocatalytic mechanism." Again, I am grateful for these very interesting suggestions. Even if the initial duplexes were not really duplexes, they would seem to have wound around each other to form the final triplex (assuming that winding around was involved in triplex formation !) I think that here lies actually the germ of the answer. The initial duplexes were not duplexes in the sense that the strands were wound around each other, and the final triplex structures did not involve any strands winding around each other either. There was no unwinding and no winding either. This would then become the explanation of denaturation. The duplexes are not composed of two strands wound around each other, but two helical strands running antiparallel alongside each other. During denaturation the two helical strands could then move apart in a linear direction. In triplex formation in a fibre, one strand moves in a linear manner from its initial partner to an adjacent duplex. Probably the helical sugar-phosphate chains would move hardly at all, but the bases might rotate to reach their new partners. I wonder if you recall the paper by Kabata et al. (SCIENCE 262 (1993) 1561-1563) ? They found that single molecules of RNA polymerase were sliding along tethered molecules of duplex DNA. The polymerase did not describe a helical locus at all. The DNA was immobilised and could not rotate and nor did the polymerase rotate either. If duplex DNA consisted of two chains wound around each other, how would the polymerase "read" the message on the alternate half turns of the duplex which lay on the other side away from the polymerase ? Would that not require two reading mechanisms, one each to be used on alternating half turns ? This would hardly be conducive to high quality transduction of the message contained in the base sequence. Kabata et al. did not address this question. If the DNA actually consisted of two antiparallel helical chains running alongside each other, the RNA polymerase could remain on the same face of the duplex and read the whole message. Attached to this note should be a diagram of the structure of duplex B-DNA, if I understand the scanner software, which I believe can account for all the experimental results with DNA which are known to me and would be very glad to consider with you what you think its limitations might be. Each sugar-phosphate helix would have the same diameter as the width of a Watson-Crick base pair, about 11 Angstroms, and the same pitch as a Watson-Crick B form double helix, about 34 Angstroms. Aaron Klug told Robert Olby in "The Path to the Double Helix", page 373, that Rosalind Franklin had been working with her Pattersons on separate helices of diameter 11 Angstroms, which would have been before she could have known about Watson-Crick base pairs (whose width is necessarily close to 11 also in order to make the geometry work). You may know Max Delbruck (Watson's tutor) wrote to Watson in 1953: "These (DNA strands) would have to be untwiddled to separate the threads....In any event, one must postulate that the DNA opens up in some manner, both for replication and for doing its business otherwise. In the structure you describe this opening up is opposed both by the two hydrogen bonds per nucleotide, and by the interlocking of the helices, and it becomes a very important consideration to find a way out of this dilemma, or to think of a modification of the structure that does not involve interlocking." Delbrück's prescience stands out across 40 years and more. I have been actively scouring the X-ray and NMR literature to bring together those many experimental results which would seem to support the suggested side-by-side model. If you would wish to follow the experimental results further along what I find a very absorbing avenue of thought do let me know. begin 666 Views of B DNA take 2.xif M24DJ`%@%``!815)/6"!$249&`@`$``(`, ````$`1 ````,`S (```0````` M````#P!#;&EV92!$96QM;VYT90`````!`( ``0#0`@`````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````!```` M`0``6 4````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M```````````````````````````````````````````````````````````` M````````````````````````$ #^``0``0```````````00``0```%8+```! M`00``0```$P0```#`0,``0````0````&`0,``0`````````1`00`& ```!X& M```6`0,``0```+$````7`00`& ```'X&```:`04``0```-X&```;`04``0`` M`.8&```>`04``0```.X&```?`04``0```/8&```H`0,``0````(````Q`0(` M& ```/X&``!*`00``0````````"JAP0``@```&2Z````````%@<``# '``!* M!P````H``-86``#$)0``#"H``(LN``")/0``/TP```1/``!B60``/FP``,5U M```2>0``.H$``)>#``#@A@``V8P``#^2``#%EP``V9P``'.=``"-G0``&@`` M`!H```"V`@``U@P``.X.``!(! ``?P0``/X.``"V#@``Q0(``%X*``#<$@`` MAPD``$T#```H" ``70(``$D#``#Y!0``9@4``(8%```4!0``F@```!H````? M````D $```$```"0`0```0````````"0`0```````) !``!V,2XP(%AEB#$N1"!L"\-K(;)&LBPY!5 2";#5\.B%A[C=7D>KVX.N@BD!CX^I% MA[?U#V&_J^&ZW^&]:]AAL(F/I+NYVECK_NX)U_W<[%- U]>[1V-BA-=43?<. M^$'7U\-.J:_]0X?3J]?M`UVO5?$<)K2:7DW!@V?KU\('T03VDKU\/PGZI=4_ M5U];R#O]`_JO4C&%_]:7_H(3H?W7UUI_6E]>O]M+5*O?_6ZKK_<+5=>GWR:! MUTNE_X7__(1'LCLCLN?M2M 1W7K@A$1(9TQ5^50%'%:^@]^""__7#M(+_KR& MP?BPI*P:%271;W1(7?!(+UW_0?JEI>D^DWT@2___+);,SWI=/_KDW50U(>@M M?I*ODW"@VH?"7^OKDW+ 5I9PP(I$,S%Z__IRSEPE$#!K^OKUT0;T+_%H$OJJ M2W7EE7"M>E_5?>&D.O^N3'Z^%(R/6M?7_%!$(&?_6_?^D0,$Z_K7UZ1%!/^O MI=>%)2??KZ7]PKK_W_KU?_2W05_P7_HFQ,,Q+]]7A>J\(AK___X2Z2Z"F!/6 MLF]3^%_7BN_R;C@WEC^B"1__6DJR; I&`:Z[A?_4F!D=5_>"%_TOI?0D5N5D MA7_X3KJ61+B-K_Z[P?Z]$-*?[ED)00IP1)56O1#3;JX?U541\<;I?"#)PT?Z M^B"HWJOZUT/]4U"7_\D@']:7^O[ZK_T@Z^G^E_X8*O_ZD8!?^*ZVU?H:6OKI M$F&=?KM>OYE@%__7(N/^E__K77^H=5__7Y-QL6%2VM>KRQ_U_KW5$/ +^O_2 M__7_\(&0V2J77^NO]=<-?],@O(1_ZU__^^M_AA?_U5)?UZL=\ $`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`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`/_0*#PDE]?__25=5]?U__TB0OKJ3OI82_^E_2]>_X7=< M(+^OKKZ]?___?Z[O__"TO_UUT3'>O__^$OO___A+U7___5?__-KU_O:K6^^E MI?_6EZ7I?_:6G_]?T_WW!!=?__2U7Z__'Z7]?>N[O06E_JO7U6O__^O]_W_\ M)?Z[U_U?__O5*E__ZW]PBAPNO__75+2___U]_Z[K^3<6!<[-4: 7,$$M>O_P MEYV9`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`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`-/Z]Z]_Z^_I>[NO^]?=[WID2%_=I.I,`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`G_ZU___]X7__"$,Z!@)KUZ27U_]__%? MPO___OKY90_WMZ8+U_071.*__I:_W_=-7_]__^_^]TU3]?1)TDE___Z___PT MO_^ONTO;_Z:IK7ZNE5?___7__R#C_WK^K_V_OZ!K?ZZ707__I__1/SD?^N+_ M_=_Z7^_\6B! ;_]+5+_U__^A(F7]?_K_]?__OJ0<'I5^J"07^O]?UT$&KK_O M]_7]WW]^[K(1(__2TDO]?7_]>_U3DM#'?O_____^$D']>JZI?__K_6H,+K\( M?__[^___R%P@7*K=?Z6%K7___ZTI!<P7 M="LE`-F___Z5>&@:25?72U_UUZ7O77__DA%.!'>X(@T?^_N]=]?(,#9#0G9\ M.E_]?_]4J[_W__P9<4P#7_D;#-?^_Z^OTB&4H!H:7KK>NO_K7____$?]H@H+ MOO[_[:77(:>A#-GK\+_[_5)4E__[^_?OH@XW_^_:VJ^A)4$AI"R7TGK]?Z5) M+5?WUWWD%W(8KWOR13VW=W?:<-+7DZ(Z`?PJK___Z5?_U]_^3!%_;7]_^T&V M%]4B$#,9`\3BQ]?_]5_Z27[_U_];]O___B0S1.OZ1$@(B*6NO_?^EI*O7__^ MO7U[__\&E6JDM&E]?_K_JE7___^E]_M_^W?@PNO0=+____=:]__N__7__W;_ M[%?I.$$JKK__JDJ26KW__MVJ6_H,^9A___ZKTL(@>#<:7___O6J_^_K?O7\C M'NA$'_^_W6J6@B#4$Z_K__>M+___WJJ_WA/_W_AA?A)($0S!9__K7^@:HG=) M*U7O__TD$V]/E.#:[_W;[#2^DH0(-I?__7R#`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`OL(7KZ<4ZI/ M_U_H(,/____LJ X2TEOOM_\B8+I8A_I0Y%[%=57_JJ0;?_WW_7.H--]=_^_E M("C]_R'%P@UU_7_\)OK2_?_J*Z___[*<&G[:KR&<:13ALI+]?^M)OO[_V_6M M5_[_\H#62UPD%"D%"3"A56M:5?H+7^O__@J22_JOKBOQJH1#5TTD$%_Q_T@0 M;K___I! R-RE327_U74@UU4OI:(X(:MT%2K^O^%#?77__I".M5]]]\AF`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`PA(&&1:K2[JO_Z?""__X3?_^[<@A2HD%$$;(9 R MB"&>%2TWZ7_J]+[_TD^_]_P7(9C*0(C23";TDJ]=)M>O:_WO_W^E2],+Z^ZU M^E_O]!!O_?[ZUIH(/>DEI?5.E7_^EOT___>U%O22_]+5)_7ZPP?_O_22#R#B MX3TJ7_:6E__VDW__OZP>#2;ZI=?2:I)?_X5_W?_T@X9 P#08:A()=?A(0J7K M_A(,/O^V]VN'+0&&4$F_2_K2JO_^F_^_OT$$1+*S+0G#*5C27_75?_^G_W_] M4$)H!B#&TDE^OI)?_ZMOW_O]$P" W:=:7^DOUW_5O=__]2#!@&Y:`(,OI?WT MDE_O])M?O>_[* R@W+04@_5?J_2__VK;___V"AART!1X5*OZ2U]_>J;^_OWX MA W%JDO_JK_]?;O_[_1#5*@[A()?Z80025^_;T@WMN[OM[:(%TH'>DO_I)+J MN_2O[_^[D"-CO227_K6J__M___Z()N>]?_I*B8_>_K2?U=O?^$'NJZ[])4'[ M__;O?[^\)!NX5??U5*NO_2;_O]WX3VX2^O2___Z0;_]_Z2>Q5?J_27_U_;[_ M_\)]?UX2PE_7WTW_W;?VDW:"]>D@H25]_?A6]OM_OT@>TB0__^U]?_IO[_O] M)O4+5>]!6E__Z3?_^_T&[27OWK(82*O_^F_^]]_>TO_(8VDUU^[ZM^0,-#]][<)KI/_J&%?_^%]R,!I7J MW](>DJ^FD5Q8,_U_V4 PWV4`K/_Z4M"D"2$*`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`$ $M!F#FL,%N M#!<]ES(61H1"C.C/#+0P[SMRM)4D@J_TG)<"^^_UHAZ8M*JI)+N_D@&M[] M=>GZ]+:JJ25GP:[_Y!N1^@\.%"2"05K)%B[28D-"3WMLBDZ7"#<,-52ID07) M/A;J4,N#LAE";]OA4E_D-01I4I!]""8M+5"*#(8++;_77]D%:9&/2JHB$_5) M`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`+UV0*S<3#72IOI+(,,I4I:AF!PV@DM! M>JN=`TOL@RFY407G2I(-_YX-I+L)_"I/PH4A!_RU;#5;2A4MMU42&Q.FH5PZ MJ$DF_Y!0DJLM4"0;""JH3;A:DBQ))1C2H*_K77#2A99L*A]=>NE]_A5A5NEH M)O4+A*%"UZMZU7K!I4X3]87UT@K(-WUTJPH?D&!R4*'JNDE4."!!"###U4+5 M+N%M]=+I6UD#!LE>O7\@0K_ZUJ%MFV1P8""#=:]69CL1"J\(H?]1PPD";&/5 M4"AD,U2!$=62%"Z5*#5"WI)_(K,T*86 SA @8/5%Q!*%+9B=N"_IZ"\'2#?" M7Z2N@4--?Z6V%#85:TJJ#$"U58.%?JP]*]+#(8*?8,-!*W2V0T"<(*##R)A+#HJ( M(%##PA"L@7"3H&B&&F"4,1!2`GH!Z,<@)C 6!AY 35@JV%("8D&;!@H_____ M______^ " " __________Y9HX;,'D-8P2'+`3FB>@:$'A$4((AF@)+19%0. M1)!)AR&F`DJ(0\'D]2&4`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`@Q) MN8#+,S_X+^NLU]>O^%UW)E3)N( PW_H$%7_PNJ____PB# %^[KU_KZ7_]?O7 M(()/_A?]4O_?_P["?]$(+Z]0T*"@IW5>OX2\+IZ^O]K?]"'#3"!5]:7587 M?_]O]Z#UT0?V4$OZ7ZZH+K_]O_7?<1#@DE^NE\KI0?_]?]?_Z23__7_[^E_H MT@E___X2\ MFQ,"Y/>OPEK_TE_^_]_?>D_EN!$N%PM5__PO7[_\?9-JPT%UH\>#(\81YD^B$%&KVA$1'V0S=A2__X7K_UU^UZ)%.N^YV*B4 MM?_^OZW__O77]1!4M+_UU_^O]+ZO_X6JU_2]?_?K_OUO^$&%_UU]+_^OZU]? M?R6?UW_T07'7_VE?U___A R&R'U_K\)___M_?]OD%QPO"(P%'_Z^$O___?=? M\1ZD##'TJ\*M!0O_PN]O^W3A^H=?7UK07^]5"?_U>O@`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`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`W_?3A!R#?9"K_7^O7___1!6E?^][\R-`:-_T]_K__KZ_W_^FG_O M?YDM!JW]]PM_]?7_DB"K___HAC"CE(_Z_N"_^G"!_ZVJ6E]809>,)$%WZ__] MW!"+ZO_^9!89IM'!__TY#-WG_Z__0B0SDB?___1.AD$A\D4>_??Y!N(_[HAB MKN#^O^__D-LLM?_WKPA$\)3__^Z($*_=OH)NF&0TS*O_^OZ!JO_]?J#]_^_\ M@@&_]N@]$"!V20-9=>M.O_DR'2?K^OTH-J^U]^]HD4=W]$(!.B&-65L-37K_ M7_2Z____U5_O^__[Z;P0/?__ZKZD6&%__?ZU_[__Z__^D&R:`U?_I?3^H.O_ M^_?NNO__K?^]/H.'K77__K;7__[]?_Z__V__].=^!=?7JO_UI5__7_I>[W__ MU[=-+H)SM :/_]?_\*@__^J____OO]]_]O[^O"U7^PA!__^^%O__O=]^]_?6 M'_U_6M=>#____]__^_O_^D'"Z277__JLL@/^M7UM/!>M^ZOU_O_^%___U]>B M7#97_[O%:)1?UNO^__;_YW@&/_^O^)8_A$4$_]U_TO^^UW_?[Z].%_7_UKKS MP.$#!___Y"Q_[W__7V__^""7_I?__0D/92O]?Z_>E__O][?[_T\$"__[ZUWT M+?UO_Z?7_[_W__W^I)<$"JO_7_K>05"]?7_]=?_^[_OW]/^"$@76.M4F_^_\ M-_M_?^M?_?_YA^_W>OR%`UZ_VO_U\D$?_Z_U?__N_ZO^_VMM01#--/JLA!5= M5]:I$4%5^E__K"7[__>E?O[R">?7;5!5[Z(*%'_K]0?_?UUKU_]_W?^T[;XC MW4$O_#[__C?U__ZU2_OO?_?__WPNM=$-,/!_JM?KU^___?O_W___[6"_KD%0 M>B&<#7_[[K_I;UNE__?_]__V&MD(6OZ(HY8XY P$Z]=?___^OOVO_K_?=_6# M-@H7_H3X:4@TCGT_TOZ___^%?__]NMWW?[!A*.NOHC S4)%___^__Z_I?^_? M^_;_^#!?UWHI`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`0=@8$&D:R(61U9!3-&2LS<2MFHR!,BPR%!H)DM+(*E_Q__?[2);G37Z;6DO__J3 M.TJ__J_?^2?(:H?KO_?^G=K7I,>EZ__I1K_Z_>K2'7(&%?]??]_VJ?Z0=+]J MJ_Z5+__?_DQPEY!<3[O__W(+C@O:I]5"=*O__T@J__]>]+5_?WOI)+22O_MUOZ_P4@G?Z:U__]Y#44$N(%^*@F.E7__]I)=))M]_WWZ[OP MFU]!!TE7_W^0:"L@P"?_[_U2__]^3'U>O]!?ZI/^_KUZ(& O__]=]>M?[_[; M]?Z03UZ3I+UO_Z!7[7__TJZU_W[OZ?]ZW^MZ7__]*__]+VNJ]?__3]5D-&JU M_]:6DO__\)A__]_ZI=)>[[\?D4#%4/TDNO2_U5?_1 @RO___\DZ]?[WO?R8! MNM6__]!/7_^OI PZ_V_O^J2_[[_[LB S:A_U?JT@<)!5__]([X,;V^E^]K3D MF&BT$$G_O]X9\&Q9`POI_6JZ"=)?_K_;O_]_[JR<-+ZW__XD,N>H/__PTGT2 M==?_K(/M'6E[__6*I4O_W^0T]*AA_3_Y`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`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`P&^D(8>$O_TDDO]]TF_>[_]$2T&Z((4V]0@OKZ_^Z_;_^W_B M@^0V#2;I*M+_7_[]*__[_D-,K>0,$TWI)+ZNJ__Z[?_[_:(9A([(9HBW]?OH M+7]_TF^][U_D%"7L'L:007____?AW?O>]O1 C<.&0U9JE6OU22"?]^]/][_? MR";/EH.&HZ26M]! @DJKU^DP[_]_PB%WJY:"X%VDE[_ZO^_]O>^_OZ#VGI+Z M]*DO_U2OW]_W03RT`0(_7_JDO______MRT"MU77ZU)NG__28=__O?">+JE_? M2I??]]-]NW_OT&])+[UJDK]:]?_]_[J_I+U^EK__T@W_N[?_W27_ZTOO_U;? M>__T$\.DEV_I0DM?>_5M7M^[;>X0;NDO2UUU__]-O_OZZAK85?_72__]6_3W MOWNAQKK^DZ__VZ3[ZWO?]K_^H9LPEM?[]-^$__W2M+_>J$A@<_K_^M7>R)!G M_W7[7_>E$?__U?92`7_]];4F_Z0-4G__V]ORG!K___23PI(?R&!R4ZU__Z3^ M3@VW]_I2T#0C""7[2Q&D5Q(&+MK^V#.!L8/B0+H3_=NZDW=40SMS&NN_2?_^ MHI^0:D_?_TBM#"![=+37_?_^Z[D&YB]^_U<'NPE_\GJ&D___3?(,"2O;??5$ M';I0/^EO]$F&P-_[^@3\D6^G^^F#N\B%*ZCZ:;_U?(I2!$C1]5VOZ\'($+@R MT"H/(9@C_W3K:O\,@0@($WI+O^X6G@Y:`0[2^E6GN07@FK?\13W6^W]D2#"A MX.,AK47J7_Y5@H!3NPL.6FG!7JZ?H(DN9ANA^M7I)=]O9## M4/ P,@T+G#(9X7?_5__(:9TV]>^[Q(98XID-->V$#6B#`VOTOD7SC_T2+4W2 MI?W<@VS08=S6/D&Y/_3>@0E/FV7!/NJOUM?Y#3T1-Z@:Y9T)QY#+$?]OH1,Q M_T[=U_^R!AP5`PE^Q M5-ZI?OP0R*;EN@P@[T2(*?^DW_^D_27?]((&^@V^A!C])]+_IO7W_JB;K(** MA.U"1* (K"3:H*MT$$K=))W^M C*$#6DV](@H?Z;U_PE?2_]^=F83X5^OTGA M$=*5)>M0F_KM[[20/]MZR4?2;QPG?7MZ^_WT=@A(/ZOI5_]+7^E?5?UZA!J" M7D$MO_^DWTO])-ZI5]O:H.RW%PS*H(G"WU7:UZ2_]/_O_Z:9;B0-7IV])*Y" M'I7]?ZOJ$K[WPD$&F6Y,-;I.VJI<:MZ7]TM]5^_Z&6XP-;PFV'H)*'H(,=!! M=\)!!W22O?X2"09;AAI>M]4D0P)5=*OKIOU>_O^0S5R/[TED-+0C/2=5_U=? M^_M40:8R#=<8UNWTL4#J]5_K>DE_OU"!X7??6DFMU6N^F__?]40N(-^_OUDK M*'NETOUI,:2_]O4)IZ_OZ4(2{I?^O___3O??8=!!?]5I?K3_WM_2M<'[ M5]4@@B"E[I)?ONDO_VN[]-ZKI+VDE__U]_Z_RT$@$;=+27238(H=?_ ML?_?_I/+09BH(-Z""20002"08B$EU#4),($$_[=TD&#"2RSO4WI4J].OP:U_ MWJ]\&J<6F^DJ55TO@R/D[(X,*UO_X5+4$M,/Z22A@@J_B(TG7;][)ZEND#4L4DJ55V MLEQ O%)RD#9?]QI/7:I+2^0?SGT(.E9&!E_N_O1;IPUH,)4DD0AM""H1UI.> M#5IOO9 BM4#T$0:UYM!):A4OTDQ(- A]_(4)2>I#-'"M!:22ODX(:KKI60:S M'_DC',C6:A#>Z4+#6A#]A)D%`MW>_2&I!0NG:2TJU2_!09!RQ_KUL*%]*D%( M(7(N$GT^@S,%#7^^J0R"ZZMUI*F(=.DNA0-;_]<%##552PW6M;[(,,N]:AAZ23?UI7YJ!K?!-*PZI)O27IV[,P;.P32AAP@D$ M@8<-))*@P^) NCL6MNE2OTDD%\AF`_UNDPK>DEH)OD- %?6&]"F_TE^07$O( M+Y6P=*W"I:IOD97N1U:+(G9%1PWI7TE23?2[U"":P 0`0"U"X:"K,E@%R;*@ M,$.*#):9%63 +DV'!"),@V2TR=DM,U&:C+(,%(D);D-B0$WI*DDW>E])?#!R M!@&0IO?JEKTO_4D#X9 PG$(,.D@DD$"8>J5Z23I]AE6C8:S#I*JJ^EPFJ7]@ MQ#";'I:=UK!?]*&PTVJ2JP]?(F&%2]X9!H"!-ZI0GZ6RK!1U_#(-584-:JF[ M2ILG#5257X8H-I*DE?]FPVJ_X83#JJ4-^E$@U75;^#";TE2;UY#7I]_EJ @% M8-A!+"03#Z2D&,,$I(*OP3])(*]4I"BW[^6HL@JO2Z3;=E_7*8% S((,.] M!A(*"(K(J93!0"@%;TOJO4*'I!%WA=<&D@WZ3ZP@R".0T#A=$,&X,._]*F34 M`AE,`H/AU5=:Q&V@HBETM*#:085>E5M GA=)O@Y"`WUUV0V$0SE]86&(EBL*A4,A MGL+Y",P%QNEOZ),Q5?Z+*0&'A M>#!Y&W"D%].'2%!DW.(4,AL,!%0!`BUO"():J0$DL#%D,- P6'80)*0$QP/, MM X>U"D!/P62T."6%!2 F0"N%AI!2 F,!I!*&D%$+"(5<8E"4(%"TH6M$&\' M'(:)+<1#__^H9-<4'____