From owner-repertoires@net.bio.net Fri Sep 08 23:00:00 1995 Path: biosci!NET.BIO.NET!biosci-help From: biosci-help@NET.BIO.NET (BIOSCI Administrator) Newsgroups: bionet.molecules.repertoires Subject: test of molreps@net.bio.net Date: 8 Sep 1995 17:47:14 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: Reply-To: biosci-help@net.bio.net NNTP-Posting-Host: net.bio.net test of molreps@net.bio.net From owner-repertoires@net.bio.net Fri Sep 08 23:00:00 1995 Path: biosci!biosci!not-for-mail From: kristoff@net.bio.net (David Kristofferson) Newsgroups: bionet.molecules.repertoires Subject: test of bionet.molecules.repertoires Date: 8 Sep 1995 17:29:18 -0700 Organization: BIOSCI International Newsgroups for Biology Lines: 11 Distribution: world Message-ID: <42qn4u$8lo@net.bio.net> NNTP-Posting-Host: net.bio.net This group is not yet ready for use. Please refrain from posting until an official opening announcement is made here. Thanks! Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-repertoires@net.bio.net Sat Sep 09 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!tank.news.pipex.net!pipex!in1.uu.net!zib-berlin.de!fu-berlin.de!news.dfn.de!uni-muenster.de!asterix.uni-muenster.de!katz From: katz@asterix.uni-muenster.de (Raphael Katz) Newsgroups: bionet.molecules.repertoires Subject: human telomerase Date: 10 Sep 1995 16:59:07 GMT Organization: Westfaelische Wilhelms-Universitaet Muenster, Germany Lines: 19 Message-ID: <42v5gr$1ib0@majestix.uni-muenster.de> NNTP-Posting-Host: asterix.uni-muenster.de X-Newsreader: TIN [version 1.2 PL2] Hallo, Wer verkauft menschliche Telomerase ? Welches Zellabor kann nach Auftrag Telomerase herstellen, z.B. durch Extraktion aus maligne entarteten Zellen gem„á Counter, Christopher M. et al., (1994) Proc. Natl. Acad. Sci. USA 91, 2900-2904 ? Who sells human telomerase, the telomere terminal transferase enzyme ? Who knows a cell laboratory, which can extract telomerase from carcinoma cells according Counter, Christo- pher M. et al., 1994 Proc. Natl. Acad. Sci. USA 91, 2900-2904 ? bye RAF From owner-repertoires@net.bio.net Sun Sep 10 23:00:00 1995 Path: biosci!biosci!not-for-mail From: biohelp@net.bio.net (BIOSCI Administrator) Newsgroups: bionet.molecules.repertoires Subject: MOLECULAR-REPERTOIRES/bionet.molecules.repertoires is ready! Date: 11 Sep 1995 16:25:26 -0700 Organization: BIOSCI International Newsgroups for Biology Lines: 206 Distribution: world Message-ID: <432gh6$t2m@net.bio.net> The MOLECULAR-REPERTOIRES/bionet.molecules.repertoires newsgroup is ready for operation. Please save these usage instructions for future reference!! IMPORTANT NOTE - The charter posted previously on bionet.announce during the voting listed the USENET name incorrectly as bionet.molecular.repertoires instead of bionet.molecules.repertoires. ^^ ^^ The newsgroup creation message sent out to news administrators contained the correct newsgroup name, so it is highly likely that the group was created correctly at your site. However, a copy of the charter including the typo was appended to the newsgroup creation message. In the event that the wrong newsgroup name appears at your site, please call this to the attention of your local news administrator. PLEASE NOTE that many USENET sites do not allow automatic creation of new USENET groups!!! If you do not see bionet.molecules.repertoires in your newsreader within another day or two, ask your news system administrator to act on our "newgroup" message to enable the group at your site. We have already done several tests and are certain that the group is currently propagating around the network. If he/she can not find the newsgroup message, have them retrieve the bionet checkgroups message from the anonymous FTP area on net.bio.net in pub/BIOSCI/doc/bionet-checkgroups-msg. This file contains the latest list of bionet USENET newsgroups and can be used to update your bionet distribution. If the newgroup did not arrive at your site, it may also be necessary for your news administrator to contact the upstream computer site providing you with your newsfeed and determine if they acted on the newgroup message. Subscribing to this group: -------------------------- IF YOU USE USENET NEWS: you need do nothing other than participate in bionet.molecules.repertoires when it appears in your newsreader. Depending upon your news software, this may entail you having to answer a prompt indicating that you want to subscribe. You might also try the command "g bionet.molecules.repertoires" in rn-like newsreaders. IF YOU ARE LOCATED IN EUROPE, AFRICA, OR CENTRAL ASIA: please send the word help in the body of your message to MXT@dl.ac.uk to retrieve general server usage instructions. To subscribe to the MOLECULAR-REPERTOIRES list, first be sure that you are sending mail from the address at which you wish to receive news postings, and then send the command SUB bionet-news.bionet.molecules.repertoires to MXT@dl.ac.uk. This message will be automatically read by the computer and your e-mail address will be extracted from the mail header and added to the list. 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This message will be automatically read by the computer and your e-mail address will be extracted from the mail header and removed from the list. IF YOU ARE LOCATED IN THE AMERICAS OR THE PACIFIC RIM: send a message to biosci-server@net.bio.net exactly as described above for subscribing except include the text unsubscribe molreps in the body of the message. Please be sure to send the message from the account whose address matches the one on the list. If your address differs, we will be notified automatically and will remove you manually from the list if we can determine what was your old address. Please contact biosci-help@net.bio.net if you have problems. IF YOU HAVE A PROBLEM: ---------------------- Please send a message to one of the following addresses depending upon your location Address Location ------- -------- biosci@daresbury.ac.uk Europe, Africa, and Central Asia biosci-help@net.bio.net Americas and the Pacific Rim and someone on the staff will help you. PLEASE DO NOT send mail to our personal e-mail addresses as this will delay a response to your request for help. How to post a message to the group: ----------------------------------- If you use news, simply post a message into bionet.molecules.repertoires. Be sure to set your "distribution" to "world" or else the message might not leave your site!! To post by e-mail, mail your message to one of the following addresses depending upon your location: Posting Address Location --------------- -------- molreps@daresbury.ac.uk Europe, Africa, and Central Asia molreps@net.bio.net Americas and the Pacific Rim and your message will be distributed automatically to everyone on the list and the USENET newsgroup. There is no editorial intervention. PLEASE DO NOT SEND SUBSCRIPTION REQUESTS TO THE POSTING ADDRESSES as you will bother everyone on the newsgroup!!! How to reply to a message on the group: --------------------------------------- If you are using a newsreader, simply use the reply or follow-up command on your newsreader (these vary from program to program) to send either private or public replies. If you are using e-mail, replies to messages that you receive will *NOT* be automatically returned to the group. This is the standard for Internet mailing lists as opposed to BITNET LISTSERVs which often send all replies back to everyone. You must be certain that your reply contains either of the two newsgroup posting addresses above in your message header if you want to share it with everyone on the group. Otherwise in most cases your reply may go back to only the original poster of the message to which you are replying. ALWAYS be certain that you examine the address on your messages before you send them!!! Once a message is sent there is no way to cancel it or bring it back!!! Some non-Internet compliant mail systems may attempt to send replies to our error-trapping address called BIOSCI-REQUEST. If yours does this, please be sure to readdress your message to molreps@net.bio.net or molreps@daresbury.ac.uk if you want to send it to the newsgroup. How to look at archives of the list: ------------------------------------ The BIOSCI archives and other BIOSCI information can be found on our WWW home page at URL http://www.bio.net/. Easy access from the WWW home page to our FTP/gopher area is available for information retrieval. Archives for MOLECULAR-REPERTOIRES/bionet.molecules.repertoires are kept in the anonymous FTP account at net.bio.net [204.31.212.2]. Look in the directory pub/BIOSCI/MOLECULAR-REPERTOIRES for posting archives. Each file is assigned a date such as 9312 for December 1993. Please note that ours is a UNIX system and all file and directory names are case-sensitive, i.e., upper case file names are different from lower case names. You can also access these same files via Gopher if you start a gopher session using net.bio.net as your gopher server. Gopher also allows you to view the individual messages within each monthly archive file. The files are in the MOLECULAR-REPERTOIRES directory. Postings to bionet.molecules.repertoires are also WAIS indexed and can be searched via either gopher or WAIS at our site. In gopher the option at net.bio.net is "Search Bionet USENET Articles" and in WAIS one should use the WAIS source biosci.src. This is a WAIS index of all BIOSCI/bionet messages including this newsgroup. Please see the BIOSCI FAQ for details. The FAQ can be requested from biosci-help@net.bio.net. Once again, if you have any administrative questions that require personal assistance, please address them to biosci-help@net.bio.net in the U.S. or biosci@daresbury.ac.uk in the UK. Best wishes for a successful newsgroup! Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-repertoires@net.bio.net Sun Sep 10 23:00:00 1995 Path: biosci!daresbury!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.molecules.repertoires Subject: test of molreps@daresbury.ac.uk Date: 11 Sep 1995 19:49:47 +0100 Lines: 3 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4320cb$hlt@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk test of molreps@daresbury.ac.uk test of molreps@daresbury.ac.uk From owner-repertoires@net.bio.net Sun Sep 10 23:00:00 1995 Path: biosci!daresbury!not-for-mail From: ajb@seqnet.dl.ac.uk Newsgroups: bionet.molecules.repertoires Subject: Test of molreps@dl: ignore Date: 11 Sep 1995 18:56:16 +0100 Lines: 5 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <431t80$f1r@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk Alan Bleasby BIONET Daresbury UK From owner-repertoires@net.bio.net Tue Sep 12 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!cs.utexas.edu!uunet!in1.uu.net!tank.news.pipex.net!pipex!lyra.csx.cam.ac.uk!cok-mac2.gen.cam.ac.uk!user From: c.okane@gen.cam.ac.uk (cok@mole.bio.cam.ac.uk) Newsgroups: bionet.molecules.repertoires Subject: Let's kick off with phage display? Date: Wed, 13 Sep 1995 13:21:00 +0000 Organization: Dept. of Genetics, University of Cambridge, UK Lines: 21 Message-ID: NNTP-Posting-Host: cok-mac2.gen.cam.ac.uk X-Newsreader: Value-Added NewsWatcher 2.0b19.1+ We're thinking here of using some of the recent (i.e., potentially high affinity) phage display libraries from Greg Winter's lab to raise antibodies against - peptides (taken from larger proteins, but just possibly also shorter neuropeptides) - E. coli expressed Drosophila proteins (pGEX vectors) We want to use them mainly for standard procedures like Westerns and immunocytochemistry, although it would be nice in the longer term to be able to block aspects of protein activity and have the option of epitope mapping the relevant bits of the protein. I just thought I would ask around about what experinces other people have had with this approach, compared to standard immunisation. Cahir c.okane@gen.cam.ac.uk -- Cahir O'Kane, Dept of Genetics, Univ. of Cambridge, UK cok@mole.bio.cam.ac.uk From owner-repertoires@net.bio.net Sun Sep 17 23:00:00 1995 Path: biosci!daresbury!not-for-mail From: "Andrew, Tel. +396-91093434" Newsgroups: bionet.molecules.repertoires Subject: repertoires and peptides Date: 18 Sep 1995 17:05:41 +0100 Lines: 61 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <43k5cl$o6r@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk In Message-Id: <199509121817.LAA01194@cmgm.Stanford.EDU> Date: Tue, 12 Sep 1995 11:20:50 -0800 renschle@cmgm.Stanford.EDU (Markus Renschler) wrote: >Hi, >I am confused about the appearance of another new bionet group called >bionet.molecules.peptides. Is the old molreps@IRBM group going to be on >bionet.molecules.repertoires or both? How will they be different? And I >guess there is no way to keep down noise, as there already is a message >regarding the availability of telomerase. > >Can you clarify those newsgroups for us? >Thanks, >Markus Renschler, Stanford University >renschle@cmgm.stanford.edu Dear Markus and others, The bionet.molecules.peptides newsgroup has been set up by the American Peptide Society and appears to be devoted almost exclusively to the study of peptide and protein chemistry (though you will have to look at their charter for details). Expect to see other newsgroups with the name bionet.molecules.whatever appearing in future. Bionet.molecules.repertoires has been created along different lines to include discussion about ALL classes of molecules which may be conceivably be produced/used as libraries, not just peptides or proteins. As such, the two groups are distinct and separate from each other, although their content is bound to overlap in some instances. Bionet.molecules.repertoires is the successor to molreps and I would encourage all those interested in continuing their participation in molreps to subscribe to the new newsgroup. Instructions on how to do this have been extensively posted by Dave Kristofferson and myself, so I will not repeat them here unless requested to do so. As far as noise is concerned it appears to be a phenomenon very much on the increase these days. If you see an off-topic message you vehemently disagree with please do not send messages to the newsgroup, biosci, or especially not to me as there is nothing I can do about this - accept it as a fact of life on the Internet for now. If you feel inclined to protest about an item please do so directly to the ORIGINAL poster of the message or their Internet provider (usually Postmaster@wherever.they.are). Hope this clarifies things a bit. Andrew |========================================================================| | Andrew Wallace | Discussion on phage display, | | | combinatorial libraries, etc. - | | IRBM P. Angeletti, | bionet.molecules.repertoires | | Via Pontina KM 30.600 | molreps@daresbury.ac.uk | | 00040 Pomezia, Italy. |-------------------------------------------| | | "It has not escaped our notice that | | Voice: +39-6-91093434 | the specific pairing we have postulated | | Fax: +39-6-91093225 | immediately suggests a possible copying | | Email: wallace@irbm.it | mechanism for the genetic material." | | | | | DISCLAIMER: I do not speak | J.D. Watson and F.H.C. Crick | | on behalf of anyone. | in Nature 171:737-737 (1953). | |========================================================================| From owner-repertoires@net.bio.net Mon Sep 18 23:00:00 1995 Path: biosci!daresbury!not-for-mail From: "Andrew, Tel. +396-91093434" Newsgroups: bionet.molecules.repertoires Subject: Postdoctoral position available Date: 19 Sep 1995 11:28:22 +0100 Lines: 10 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <43m606$i8e@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk Forwarded with permission: Postdoctoral position available immediately for an individual interested in the in vitro evolution of DNA binding proteins and their development as a strategy for gene therapy. Applicants should have a strong background in the characterization of DNA binding proteins, determination of affinity, foot printing, reporter gene assays etc. Send curriculum vitae and three references to:Dr. C.F. Barbas. Department of Molecular Biology, MB-11, The Scripps Research Institute, 10666 North Torrey Pines Road., La Jolla, CA 92037 , Fax(619) 554-6778. From owner-repertoires@net.bio.net Tue Sep 19 23:00:00 1995 Path: biosci!daresbury!not-for-mail From: "Andrew, Tel. +396-91093434" Newsgroups: bionet.molecules.repertoires Subject: Question about peptoids Date: 20 Sep 1995 18:33:47 +0100 Lines: 47 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <43pj9r$qv4@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk, mol-diversity@listserv.arizona.edu Dear All, Is anyone out there working with peptoids? If so, are you able to synthesise mono- and di- peptoids without difficulty? I have managed to synthesise a tripeptoid by "submonomer" synthesis as described in the Chiron paper on the subject, the compound in question being CHIR 2279 which I obtained with NMR and mass spec data identical to that reported, however when I tried to make a few monopeptoids I encountered some problems: 1) N-(4-aminobiphenyl)glycinamide -> 1H-NMR OK but low yield. 2) N-(2,2-diphenylethyl)glycinamide -> as in 1) 3) N-(4-phenoxyanilyl)glycinamide -> NMR shows possible rearrangement to a disubstituted aromatic species with no sign of the second aromatic ring. We only found 2 triplet and 2 doublet proton signals in the aromatic region. NMR of the starting amine 4-phenoxyaniline showed the correct compound. The above were all obtained after cleavage from a Rink-TentaGel support with 95% TFA/5% water, as described by Chiron. Has anyone else seen similar things, especially when working with 4-phenoxyaniline derivatives? Apart from this I have heard that, in general, it can be difficult to obtain mono- and dipeptoids from solid-phase synthesis, although tripeptoids work well. What are your experiences? Any advice/information gratefully received. Andrew |========================================================================| | Andrew Wallace | Discussion on phage display, | | | combinatorial libraries, etc. - | | IRBM P. Angeletti, | bionet.molecules.repertoires | | Via Pontina KM 30.600 | molreps@daresbury.ac.uk | | 00040 Pomezia, Italy. |-------------------------------------------| | | "It has not escaped our notice that | | Voice: +39-6-91093434 | the specific pairing we have postulated | | Fax: +39-6-91093225 | immediately suggests a possible copying | | Email: wallace@irbm.it | mechanism for the genetic material." | | | | | DISCLAIMER: I do not speak | J.D. Watson and F.H.C. Crick | | on behalf of anyone. | in Nature 171:737-737 (1953). | |========================================================================| From owner-repertoires@net.bio.net Tue Sep 19 23:00:00 1995 Path: biosci!daresbury!not-for-mail From: "Andrew, Tel. +396-91093434" Newsgroups: bionet.molecules.repertoires Subject: Mol-Diversity listserv Date: 20 Sep 1995 17:13:59 +0100 Lines: 62 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <43pek7$mt4@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk Dear All, You may be interested to know that there is another news list devoted to the topic of repertoires, it is called mol-diversity and is to be found on the listserv at arizona university. It seems to be mainly oriented towards chemistry and chemical libraries, so you molecular biologists may not be into this too much, but take a look and see what you think. Here is some information from the moderator of the list. ========================================================================= Welcome to Mol-Diversity Dear Subscriber: This list was set up in aim to facilitate the free exchange of ideas and information resources among molecular diversity researchers all over the world. As a unmoderated one, this list can be used for discussions in any topics related to molecular diversity for basic research and drug discovery. Any suggestions and comments about this forum and the issues covered by this forum are highly appreciated. The key to the success of such a list is its members' active participation. As a new member, you are encouraged to send a short message to the list introducing who you are, what you expect from the list, and, if possible, listing your recent publications for bibliographic exchanges. If you have any questions about this list, please contact Zhan G. Zhao at Zhao@biosci.arizona.edu ------------------------------------------------------------------------- To subscribe to the list, send mail as follows: To: listserv@listserv.arizona.edu Subj: (none) With the command: SUB mol-diversity your name in the body of the message. You will then receive the above welcome message and further instructions on how to participate. Andrew |========================================================================| | Andrew Wallace | Discussion on phage display, | | | combinatorial libraries, etc. - | | IRBM P. Angeletti, | bionet.molecules.repertoires | | Via Pontina KM 30.600 | molreps@daresbury.ac.uk | | 00040 Pomezia, Italy. |-------------------------------------------| | | "It has not escaped our notice that | | Voice: +39-6-91093434 | the specific pairing we have postulated | | Fax: +39-6-91093225 | immediately suggests a possible copying | | Email: wallace@irbm.it | mechanism for the genetic material." | | | | | DISCLAIMER: I do not speak | J.D. Watson and F.H.C. Crick | | on behalf of anyone. | in Nature 171:737-737 (1953). | |========================================================================| From owner-repertoires@net.bio.net Wed Sep 20 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!newsserver.jvnc.net!newsserver2.jvnc.net!netnews.upenn.edu!bb3.med.upenn.edu!user From: siegeld@mail.med.upenn.med (Don L. Siegel, Ph.D., M.D.) Newsgroups: bionet.molecules.repertoires Subject: Postdoctoral position available in Philadelphia, PA, USA Date: 20 Sep 1995 20:48:32 GMT Organization: Department of Pathology, University of Pennsylvania Lines: 10 Message-ID: NNTP-Posting-Host: bb3.med.upenn.edu Dr. Don Siegel (SiegelD@mail.med.upenn.edu) wishes to announce the opening of an NIH postdoctoral position in his laboratory in the Department of Pathology & Laboratory Medicine at the University of Pennsylvania School of Medicine for an individual interested in examining the human Ig repertoire used to encode anti-red blood cell antibodies and in characterizing their respective antigenic epitopes. Both cellular and recombinant phage display methods are to be utilized to express human anti-red blood cell antibodies and to develop novel inhibitory peptide mimetics with therapeutic immunomodulatory potential. For more information contact Dr. Siegel by E-mail or at 215-898-9655. From owner-repertoires@net.bio.net Thu Sep 21 23:00:00 1995 Path: biosci!daresbury!not-for-mail From: "Andrew, Tel. +396-91093434" Newsgroups: bionet.molecules.repertoires Subject: Fwd: Conference announcement by Scott D. Kahn of Biosym/Msi Date: 22 Sep 1995 15:55:20 +0100 Lines: 39 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <43uioo$5ek@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk ----------------------------------------------------------------------------- The following call for papers has been posted to several list servers. Sorry for any redundant messages (in advance). >> CALL FOR PAPERS << --------------- I am organizing a symposium for the Division of Chemical Information (CINF) of the ACS entitled "Managing Information in Databases of Three-Dimensional Structures" at the Spring ACS meeting in New Orleans, 24-29 March 1996. I would like to broadly focus of the symposium on the several aspects of _3D_ information management (e.g. integration with modeling/relational data, generation/storage of 3D structures, flexibiliy in searching, efficient indexing methods, etc.), and especially those that are potentially problematic as the compound generation process becomes more automated (i.e., via combinatorial methods). All _scientific_ presentations are welcomed; sales/ marketing presentations are actively discouraged (commercial and academic alike!). If you would like to contribute to this symposium, please send me four (4) copies of a 150 word abstract, including the original ACS abstract form, before 30 October 1995 at: Scott D. Kahn Biosym/Molecular Simulations 555 Oakmead Parkway Sunnyvale, California 94086-4023 Phone: (408) 522-0100 (secretary) FAX: (408) 522-0199 Email: skahn@msi.com or sdk@biosym.com ACS abstract forms can be obtained from me or from ACS Headquarters (Tel. 202-872-4396; email: natlmtg@acs.org). ------ Scott D. Kahn, Biosym/MSI (408) 522-0100 Email : scott@ba.msi.com or skahn@msi.com FAX (408) 522-0199 From owner-repertoires@net.bio.net Sun Sep 24 23:00:00 1995 Path: biosci!daresbury!not-for-mail From: "Janet Clench, Library, Tel:(39 6)91093220" Newsgroups: bionet.molecules.repertoires Subject: Molrep references Date: 25 Sep 1995 15:21:09 +0100 Lines: 118 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <446dsl$3uv@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk Dear old and new list members, Here below is a 3-week batch of references relevant to the Molecular Repertoires, Combinatorial & Phage Libraries field. Once a week (except on highdays and holidays) I search the new Current Contents diskettes available here at IRBM, and then put the new search up for your bibliography. Ciao! Janet **************************************************** SUBJECT: Combinatorial & Phage Libraries DATE: 11, 18, 25 September, 1995 **************************************************** Analytical Chemistry 67: 17 (SEP 1 1995) Y Dunayevskiy, P Vouros, T Carell, EA Wintner, J Rebek Characterization of the complexity of small molecule libraries by electrospray ionization mass spectrometry 2906-2915 Tetrahedron Letters 36: 34 (AUG 21 1995) M Goebel, I Ugi Beyond peptide and nucleic acid combinatorial libraries-applying unions of multicomponent reactions towards the generation of carbohydrate combinatorial libraries 6043-6046 Biochemistry 34: 33 (AUG 22 1995) W Tegge, R Frank, F Hofmann, WRG Dostmann Determination of cyclic nucleotide-dependent protein kinase substrate specificity by the use of peptide libraries on cellulose paper 10569-10577 Journal of Biological Chemistry 270: 34 (AUG 25 1995) IM Takenaka, SM Leung, SJ Mcandrew, JP Brown, LE Hightower Hsc70-binding peptides selected from a phage display peptide library that resemble organellar targeting sequences 19839-19844 Journal of Cell Biology 130: 5 (SEP 1995) R Pasqualini, E Koivunen, E Ruoslahti A peptide isolated from phage display libraries is a structural and functional mimic of an RGD-binding site on integrins 1189-1196 Thrombosis and Haemostasis 74: 1 (JUL 1995) LLK Leung Application of combinatorial libraries and protein engineering to the discovery of novel anti-thrombotic drugs 373-376 Journal of Biotechnology 41: 2-3 (JUL 31 1995) R Rigler Fluorescence correlations, single molecule detection and large number screening - Applications in biotechnology 177-186 Journal of Biotechnology 41: 2-3 (JUL 31 1995) M Little, F Breitling, S Dubel, P Fuchs, M Braunagel Human antibody libraries in Escherichia coli 187-195 Journal of Biotechnology 41: 2-3 (JUL 31 1995) R Frank Simultaneous and combinatorial chemical synthesis techniques for the generation and screening of molecular diversity 259-272 Molecular Medicine Today 1: 4 (JUL 1995) J Eichler, RA Houghten Generation and utilization of synthetic combinatorial libraries 174-180 Protein Engineering 8: Suppl. (1995) D Lener, F Felici, R Benarous, RA Calogero Use of a phage peptide library to isolate peptide sequences binding HIV-1 nucleocapsid protein (NCp7) 4 Protein Engineering 8: Suppl. (1995) CI Wang, Q Yang, CS Craik Isolation of a high affinity inhibitor of uPA by phage display of ecotin 84 Journal of Medicinal Chemistry 38: 16 (AUG 4 1995) GT Wang, S Li, N Wideburg, GA Krafft, DJ Kempf Synthetic chemical diversity: Solid phase synthesis of libraries of C-2 symmetric inhibitors of HIV protease containing diamino diol and diamino alcohol cores 2995-3002 Hybridoma 14: 4 (AUG 1995) P Kermani, L Peloquin, J Lagace Production of ScFv antibody fragments following immunization with a phage-displayed fusion protein and analysis of reactivity to surface-exposed epitopes of the protein F of Pseudomonas aeruginosa by cytofluorometry 323-328 Journal of Virology 69: 9 (SEP 1995) S Mattioli, L Imberti, R Stellini, D Primi Mimicry of the immunodominant conformation-dependent antigenic site of hepatitis A virus by motifs selected from synthetic peptide libraries 5294-5299 Gene 160: 2 (JUL 28 1995) E Soderlind, M Vergeles, CAK Borrebaeck Domain libraries: Synthetic diversity for de novo design of antibody V-regions 269-272 From owner-repertoires@net.bio.net Tue Sep 26 23:00:00 1995 Path: biosci!BIOC01.UTHSCSA.EDU!raman From: raman@BIOC01.UTHSCSA.EDU (C.S.RAMAN) Newsgroups: bionet.molecules.repertoires Subject: CDR3 Date: 27 Sep 1995 16:22:28 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 34 Sender: daemon@net.bio.net Distribution: world Message-ID: <9509272319.AA06797@bioc01.uthscsa.edu> NNTP-Posting-Host: net.bio.net Hello: Has anyone come across hydrophobic amino acid clusters in the CDRs (particularly CDR3) of heavy chain repertoires? For that matter, is there documented evidence for very hydrophobic CDRs in general. My understanding (via Padlan's review) is that aliphatic amino acids are the least represented in the CDRs. Any information on this subject would be highly appreciated. Cheers -raman -- _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ _/ _/ _/ C.S.RAMAN _/ _/ Department of Biochemistry _/ _/ University of Texas Health Science Center _/ _/ 7703 Floyd Curl Drive _/ _/ San Antonio, TX 78284-7760 _/ _/ USA _/ _/ _/ _/ Tel: (210) 567-6623 _/ _/ Fax: (210) 567-6595 _/ _/ E-mail: raman@bioc01.uthscsa.edu _/ _/ _/ _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ _/ _/ _/ How can it be that mathematics, a product of human thought _/ _/ independent of experience, is so admirably adapted to the _/ _/ objects of reality? -Albert Einstein _/ _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ From owner-repertoires@net.bio.net Wed Sep 27 23:00:00 1995 Path: biosci!daresbury!not-for-mail From: "Andrew, Tel. +396-91093434" Newsgroups: bionet.molecules.repertoires Subject: Meeting Report on Solid-Phase Synthesis, Edinburgh 1995 Date: 27 Sep 1995 19:08:29 +0100 Lines: 86 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <44c3ut$2pc@mserv1.dl.ac.uk> X-Vmsmail-Cc: WALLACE Original-To: ACALI@IRBM.IT, ALONZI@IRBM.IT, ALTAMURA@IRBM.IT, AMATI@IRBM.IT, AQUILINA@IRBM.IT, BARBATO@IRBM.IT, BARTHOLOMEW@IRBM.IT, BARTOLI@IRBM.IT, BAZZO@IRBM.IT, BEHRENS@IRBM.IT, BIANCHI@IRBM.IT, BRENNAN@IRBM.IT, BRUNIERCOLE@IRBM.IT Elisabetta Bianchi and Andrew Wallace - Meeting Report Edinburgh 1995 Report on the 4th International Symposium on Solid-Phase Synthesis and Combinatorial Chemical Libraries University of Edinburgh, 12th - 16th September 1995. This meeting was run as a series of concurrent sessions divided between the two themes Peptide Chemistry / Combinatorial Libraries and Nucleic Acid Chemistry / Glycoproteins. In most sessions there was a significant presence of speakers from industry or with industrial connections and as a result, few novel findings were presented; there was also a notable absence of questions and discussion after many presentations. There were, however, a number of interesting highlights which we report below: Tony Johnson from the MRC-LMB in Cambridge (group of R. Sheppard) presented some work on the use of backbone-protected peptide fragments for the synthesis of large polypeptides by fragment condensation strategies. In particular, they have discovered that the use of acetylated-2-hydroxy-4-methoxybenzyl groups attached to the amide nitrogen of protected peptides imparts remarkable solubilising properties facilitating purification by standard HPLC and permits the use of high concentrations of coupling fragments in condensation reactions. The synthesis of the Tat protein from HIV was described, as well as other proteins of up to 126 residues. Steven Kates from PerSeptive Biosystems described the use of a novel onium reagent, tetramethylfluoroformamidinium hexafluorophosphate (TFFH) for the in situ preparation of Fmoc-amino acid fluorides. This is compatible with the use of Arg(Pmc) and His(Trt), which cannot normally be isolated as the acid fluoride. The TFFH solution is stable in DMF for up to 3 weeks. Murray Goodman from UC San Diego described the use of urethane N-carboxyanhydrides of Fmoc-amino acids in the context of automated synthesis on an ABI instrument, permitting the use of coupling cycles as short as 15 minutes. One point of interest about combinatorial libraries was that thrombin seems to have entered into the list of favourite targets for finding active compounds from a library, along with the mu-opioid receptor. There were presentations from Richard Houghten, John Chabala (Pharmacopea) and Dan Cook (Isis pharmaceuticals), all of whom found active compounds against one or both of these targets. In addition there were presentations from Glaxo/Affymax (photolithography libraries), Sphinx Pharmaceuticals (biphenyl libraries by solid-phase Stille coupling, recently published) and Affymax (2+2 cycloaddition chemistry on solid-phase, again recently published). Holger Wenschuh from the Institute of Molecular Pharmacology, Berlin described the use of amino acid fluorides for the incorporation of hindered residues such as N-methylated amino acids or alpha-alpha disubstituted residues into peptides or peptide alcohols (peptaibols). Slow coupling of some hindered Fmoc-amino acids can lead to Fmoc-removal and oxazolone formation. This can be prevented by coupling in nonpolar solvents and silylation of the acceptor amino group prior to the coupling reaction. George Barany, University of Minnesota described a novel procedure for the formation of intramolecular disulphide bridges both in solution and on solid-phase by using either a solid-phase form of Ellman's reagent or various N-dithiasuccinoyl amines. Some poster presentations of interest: Immobilisation of histidine tagged peptides on nickel chelate derivatized microtitre plates from Rasmussen and coworkers of Nunc, Denmark. They described a new nitrilotriacetic acid derivative with anthraquinone which can be photochemically attached to polystyrene because quinones readily covalently attach to polymers upon UV irradiation. Photochemical coupling of peptides to polystyrene microwell plates by the same group from Denmark. The peptides to be covalently immobilised were acylated on solid-phase synthesis by N-carboxypentylanthraquinone-2-carboxamide as a photoprobe for the subsequent UV immobilisation step in the microwells. Selection of proteinase inhibitors from a conformationally constrained combinatorial library, from the group of Robin Leatherbarrow of Imperial College, London. They synthesised a library of 8000 disulphide-linked loops from the inhibitory region of the Bowman Birk Inhibitor protein on TentaGel beads and used this support-bound library to screen for inhibitors of chymotrypsin. Three out of nine positions were randomised and the library was cyclised on the solid support by oxidising in 20% DMSO, 5% acetic acid for 48 hours. From owner-repertoires@net.bio.net Thu Sep 28 23:00:00 1995 Path: biosci!daresbury!not-for-mail From: "Andrew, Tel. +396-91093434" Newsgroups: bionet.molecules.repertoires Subject: CV posting on behalf of Galina Kuzmicheva Date: 28 Sep 1995 20:34:11 +0100 Lines: 188 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <44etbj$ejd@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk, mol-diversity@listserv.arizona.edu, ilyichev@vector.nsk.su Dear All, I have been asked to post the following CV by a Russian scientist who is looking for a postdoc (about 1 year) in a good lab working on phage display technology anywhere outside Russia. Please pass this on to anyone who you think may be interested. Andrew =========================================================================== CURRICULUM VITAE NAME: Galina A. Kuzmicheva PRESENT POSITION AND ADDRESS: Senior Research Scientist, Research Institute of Gene Engineering State Research Center of Virology and Biotechnology "Vector" Koltsovo, Novosibirsk Region, 633159, Russia. HEAD OF DEPARTMENT: Russian Ministry of Public Health and Medical Industry FAX NUMBER: (383-2)-328831, TEL: (383-2)-647916 E-mail: ilyichev@vector.nsk.su (Galina Kuzmicheva) PERSONAL: Date and Place of Birth: July 18, 1956 Nicolaevka, Saratov Region, USSR Marital Status and Children: single Home Address: Galina Kuzmicheva, Russkaja Ave. 25-5, Novosibirsk, 630058, Russia PROFESSIONAL EXPERIENCE: 1994-present Senior Research Scientist, Research Institute of Gene Engineering State Research Center of Virology and Biotechnology "Vector", Koltsovo, Novosibirsk Region, Russia Projects and participation: 1. Mapping of antigenic determinants of Venezuelan equine encephalomyelitis virus (VEE, E2 protein) using phage display epitope libraries (the direct performer). 2. Selection of HBsAg mimotopes (Hepatitis B virus) from phage display libraries (the direct performer). 3. Construction gene-targeted phage display libraries, containing fragments of E2 protein of VEE (the main performer). 3. Construction derivative f88-4, displaying antigen determinant (232-240) E2 protein of VEE (in collaboration). 4. Development new vector M13, containing wild and recombinant gene VIII - M13-88 for phage display (in collaboration). 1994-1992 Header of Biochemical Laboratory, Novosibirsk Serpentry, Novosibirsk, 630111, Russia Projects and participation: 1. Development of methods of purification of native snake venoms (the project leader and the direct performer) 2. Developments of methods of crystallization and liophylization of native snake venoms (the project leader and the direct performer) 3. Investigation biological and enzymatic activities of dry snake venoms and development of chromatographer methods of separation of snakes venoms (in collaboration) 1992-1978 Research Scientist, Research and Technology Institute of Biologically Active Substances, "Vector", Berdsk, Novosibirsk Region, Russia Main projects and participation: 1. Investigation mutagenic properties of random oligonucleotides in Ames tests (the project leader and the performer executor). 2. Investigation mutagenic property of oligonucleotides with modified sugar-phosphate backbones using filamentous phage systems (the direct performer). 3. Protein design of short peptides by oligonucleotide directed mutagensis (in collaboration). 4. Development procaryotic genetic constructions, containing the encephalitic determinant S1 of myelin basic protein (the performer executor). 5. Construction and design of procaryotic vectors, containing the synthetic tryptophan and tac-promotores and investigation their promoter activities (the direct performer). 6. Chemico-enzymatic synthesis and cloning of the adrenocorticotropic hormone genetic equivalent fragments in E.coli (the direct performer). 7. Studies on the direct expression of genes of short peptides in Escherichia coli (in collaboration) 1983 - 1982. School of Molecular Biology and Gene Engeneering, Department of Moscow State University. Institute of Protein. Puschino, Moscow Region. Russia. Creation DNA-fragments library of genome of Thermus thermophilis in lamda package vector (graduation work). EDUCATION: Ph.D. 1992 Molecular biology, Institute of Molecular Biology "Vector", Koltsovo, Novosibirsk Region, Russia M.S. (equivalent) 1983 School of Molecular Biology and Gene Engeneering, Department of Moscow State University. Institute of Protein. Puschino, Moscow Region. Russia. M.S. 1978 (with Honor) Kuibichev State University, Chemical-biological Department Samara (Kuibchev), Russia MAIN PUBLICATION 1988-present in Russian (Abstracts in English) 1.Petrenko V.A., Kuzmicheva G.A., Tatkov S.I., Krasnoborov I.I., Pozdnyakov P.I.,Ilyichev A.A., Baturina I.I., Homov V.V. Study of the mutagenesis mechanism directed by phosphotriester analogs of oligonucleotides. The role of repair in mutating. Molekulyarnaya Biologiya. 1990. V.24. N.2 pp.460-466. 2.Petrenko V.A., Kuzmicheva G.A., Tatkov S.I., Krasnoborov I.I., Ilyichev A.A., Baturina I.I., Maistrenko V.F., Citovich A.V., Dolinnaya N.G., Shabarova Z.A. Mutagenesis directed by oligonucleotides containing modificated internucleotide bonds. I. The role of ribonucleotides in repair and replication of DNA. Molekulyarnaya Biologiya. 1990. V.24. N.5 pp. 1332-1338. 3.Petrenko V.A., Kuzmicheva G.A., Tatkov S.I., Krasnoborov I.I., Ilyichev A.A., Drutsa V.L., Shabarova Z.A. Investigation of mutagenic property of oligonucleotides with pirophosphate link using bacteriophage M13 system. Molekulyarnaya Biologiya. 1991. V.25.N 6 pp. 1539-1545. 4.Petrenko V.A., Kuzmicheva G.A., Tatkov S.I. Investigation of mutagenic property of modified oligonucleotides. All-Union Meeting of NC-duplexes. Kiev.1989. 5.Petrenko V.A., Kuzmicheva G.A., Karpenko L.I., Tatkov S.I., Ilyichev A.A. Investigation of mutagenic property of oligonucleotides analogues."Synthetic oligonucleotides: problems and frontiers of practical application". Moscow USSR June 23-30. 1991. p.96. 6.Petrenko V.A., Karpenko L.I., Tatkov S.I., Smirnova O.Yu., Kuzmicheva G.A., Ilyichev A.A. Mutagenesis induced by oligonucleotide phosphotriester analogues and directed at double-stranded DNA breaks. Molekulyarnaya genetika, microbiologiya i virusologiya. 1991. N 10.pp. 19-22. 7.Baturina I.I., Belova N.V., Belyavskaya V.A., Zagrebelny S.N., Zakabunin A.I., Kuzmicheva G.A., Melamed N.V., Putintseva N.I., Saikovich E.G.,Yakushenko V.V., Yasnetskaya S.M. Studies on the direct expression of peptide genes in Echerichia coli. I. Chemico-enzymatic synthesis and cloning of the adrenocorticotropic hormone ACTG 4-10 genetic equivalent fragment. Biotechnologiya 1988. V.4. N 2. pp.183-188. 8.Kuvshinov B.N., Kuzmicheva G.A. Masicheva B.I., Makshutov A.I, Nadolinnaya I.G., Petrenko B.A., Ilychev A.A. Molecular mimicry as a cause of induction of autoimmune diseases. The study of encephalytogenicity of recombinant proteins containing S1-determinant of myelin basic protein. Molekulyarnaya genetika, microbiologiya i virusologiya. in press REFERENCES 1.Prof. V.A.Petrenko. University of Missouri-Columbia Division of Biological Sciences 406 Tucker Hall Columbia, MO 65211 U.S.A. FAX NAMBER: 13148820123 TEL: 13148823344, 13148822720 E-mail: petrenk@biosci.mbp.missouri.edu 2.Prof.L.S.Sandakhchiev. State Research Center of Virology and Biotechnology "Vector", Koltsovo, Novosibirsk Region, 633159, Russia. FAX NUMBER: (383-2)-328831 TEL: (383-2)-328942 E-mail: vector@vector.nsk.su 3.Dr.A.A.Ilyichev. Research Institute of Gene Engineering State Research Center of Virology and Biotechnology Vector NPO, Koltsovo, Novosibirsk Region,633159 Russia. FAX NUMBER: (383-2)-328831 TEL: (383-2)-647815 E-mail: ilyichev@vector.nsk.su