From owner-repertoires@net.bio.net Mon Dec 04 22:00:00 1995 Path: biosci!daresbury!not-for-mail From: "Andrew, Tel. +396-91093434" Newsgroups: bionet.molecules.repertoires Subject: Test message - do not read Date: 4 Dec 1995 18:58:55 -0000 Lines: 1 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <49vgdf$ir0@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk This is just a test. From owner-repertoires@net.bio.net Tue Dec 05 22:00:00 1995 Path: biosci!agate!usenet.kornet.nm.kr!usenet.hana.nm.kr!usenet.seri.re.kr!news.dacom.co.kr!nntp.coast.net!harbinger.cc.monash.edu.au!lugb.latrobe.edu.au!newsmgr From: Mick Foley Newsgroups: bionet.molecules.repertoires Subject: storing phage at 4oC Date: 6 Dec 1995 02:25:31 GMT Organization: LaTrobe University Lines: 11 Message-ID: <4a2uur$vna@lugb.latrobe.edu.au> References: <49vgdf$ir0@mserv1.dl.ac.uk> NNTP-Posting-Host: bcgf1.latrobe.edu.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Hi all, We have been storing phage expressing a scFv on gene III at 4oC and find that afetr 4-5 days we get loss of binding to the antigen. Even after PEG precipitation. Can anyone suggest the best way to store them at 4oC so they remain useful for assays etc. Cheers, Mick From owner-repertoires@net.bio.net Fri Dec 08 22:00:00 1995 Path: biosci!ihnp4.ucsd.edu!agate!howland.reston.ans.net!nntp.coast.net!news00.sunet.se!sunic!news99.sunet.se!news.rccn.net!gnu.mat.uc.pt!histocentro From: psantos@gemini.ci.uc.pt (Paulo Santos) Newsgroups: bionet.molecules.repertoires Subject: Biosensors (MHC-peptide-TCR) Date: 9 Dec 1995 18:19:27 GMT Organization: LUSOTRANSPLANTE Lines: 25 Message-ID: <4acjvf$4vr@gnu.mat.uc.pt> NNTP-Posting-Host: histocentro.uc.pt X-Newsreader: News Xpress Version 1.0 Beta #3 I would like to have some information about biosensors and it's use for analysing molecules interaction. My special interest is the aplication to the MHC-peptide and TCR affinities (study the interaction of a special MHC allele and a pool of naturally or synthetic peptides, or vice-versa, a special peptide and a pool of MHC alleles). Thanks in advance. Paulo Santos Laboratorio de Genetica Molecular Centro de Histocompatibilidade do Centro LUSOTRANSPLANTE Faculdade de Medicina da Universidade de Coimbra, piso 3 Apartado 3004 P-3049 COIMBRA Codex PORTUGAL Telefone +351-39-33693 +351-39-20527 Fax +351-39-33674 Email: psantos@gemini.ci.uc.pt From owner-repertoires@net.bio.net Mon Dec 11 22:00:00 1995 Path: biosci!daresbury!not-for-mail From: devant@merck.de (devant) Newsgroups: bionet.molecules.repertoires Subject: Postdoctoral Fellowship Date: 12 Dec 1995 06:41:11 -0000 Lines: 48 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4aj867$fui@mserv1.dl.ac.uk> X-Sender: devant@wingate.merck.de Original-To: molreps@dl.ac.uk Hi everyone out there! You have just finished your Phd and you are looking for a position as a postdoctoral fellow in the German pharmaceutical industry? Well - if you are working in the field of non-peptidic combinatorial chemistry you might be just the person we are looking for. Recently, within the Department of Medicinal Chemistry of Merck KGaA (Darmstadt, Germany), a small group of chemists started to enter the magic field of combinatorial chemistry and we are looking for someone experienced in solid phase synthesis to join us as a postdoctoral fellow (beginning with 1 year with the option of a second year). Our interest, besides of the automated synthesis of millions and millions of compounds for biological screening, is to bring the vast world of preparative organic chemistry onto the solid phase. Your start here should be in the first quarter next year. Sounds interesting to you and fits your plans? Need more information? Just drop an email to the address given below. Explore also http://www.merck.de and http://www.th-darmstadt.de/darmstadt.html to get an idea how it is like here. Ralf M. Devant ---------------------------------------------------------------------------- --------------------------------------------------------------------- Dr. Ralf M. Devant Merck KGaA Med. Chem. Res. Labs / CNS Department Frankfurter Str. 250 64271 Darmstadt/Germany email: devant@merck.de Tel. (49) 6151 727548 FAX (49) 6151 710757 ---------------------------------------------------------------------------- -------------------------------------------------------------------- From owner-repertoires@net.bio.net Sat Dec 16 22:00:00 1995 Path: biosci!agate!news.Stanford.EDU!nntp-hub2.barrnet.net!snooze.ser.bbnplanet.com!crick.sura.net!news.ums.edu!umabnet.ab.umd.edu!beaker.ab.umd.edu!user From: pemanuel@umabnet.ab.edu (Peter) Newsgroups: bionet.molecules.repertoires Subject: Exprssn Vector for Fab in Bact? Date: Fri, 15 Dec 1995 14:46:30 -0500 Organization: UMAB Lines: 15 Message-ID: NNTP-Posting-Host: beaker.ab.umd.edu Can anyone suggest a vector for the expression of recombinant Fabs in bacterial systems. The vector should have the ability to allow for induction with no leaky expression in the uniduced state. Carb or Amp resistance is a plus as is Not/ Spe sites for insertion of protein encoding antibody genes. Any suggestions are welcome. Peter -- Peter Emanuel Ph.D. University of Maryland School of Medicine 410-706-429410-706-8012 FAX From owner-repertoires@net.bio.net Tue Dec 19 22:00:00 1995 Path: biosci!agate!usenet.kornet.nm.kr!usenet.hana.nm.kr!usenet.seri.re.kr!news.dacom.co.kr!nntp.coast.net!harbinger.cc.monash.edu.au!lugb.latrobe.edu.au!newsmgr From: Mick Foley Newsgroups: bionet.molecules.repertoires Subject: rat oligos Date: 19 Dec 1995 23:43:55 GMT Organization: LaTrobe University Lines: 14 Message-ID: <4b7inr$rm3@lugb.latrobe.edu.au> NNTP-Posting-Host: bcgf1.latrobe.edu.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Hi all, Does anyone have any information on the availability of either primers or the sequences of the appropriate primers for amplifying scFvs from rat McAbs. I am particularly interested in IgG(2a). We have such a monoclonal and want to clone a scFv or Fab. Thanks for your help. Cheers, Mick From owner-repertoires@net.bio.net Wed Dec 27 22:00:00 1995 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molecules.repertoires Subject: BIOSCI miniFAQ, ver. 14-DEC-95 Date: 28 Dec 1995 02:00:17 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 199 Sender: daemon@net.bio.net Distribution: world Message-ID: <199512281000.CAA12860@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 14-DEC-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. Contents: -------- 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index in addition to the master index for the entire set. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net