From owner-repertoires@net.bio.net Wed Jan 03 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: "Andrew, Tel. +396-91093434" Newsgroups: bionet.molecules.repertoires Subject: Question about Fab vectors Date: 4 Jan 1996 18:06:32 -0000 Lines: 24 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4ch4v8$ks9@mserv1.dl.ac.uk> Original-To: pemanuel@umabnet.ab.edu, molreps@dl.ac.uk Dear Peter, Did you get any replies to your question about vectors for the expression of Fabs? If you did, it might be helpful to others to post a summary of what you found out. Thanks, Andrew |========================================================================| | Andrew Wallace | Discussion on phage display, | | | combinatorial libraries, etc. - | | IRBM P. Angeletti, | bionet.molecules.repertoires | | Via Pontina KM 30.600 | molreps@daresbury.ac.uk | | 00040 Pomezia, Italy. |-------------------------------------------| | | "It has not escaped our notice that | | Voice: +39-6-91093434 | the specific pairing we have postulated | | Fax: +39-6-91093225 | immediately suggests a possible copying | | Email: wallace@irbm.it | mechanism for the genetic material." | | | | | DISCLAIMER: I do not speak | J.D. Watson and F.H.C. Crick | | on behalf of anyone. | in Nature 171:737-737 (1953). | |========================================================================| From owner-repertoires@net.bio.net Sun Jan 07 22:00:00 1996 Path: biosci!IMB.IMB.AC.RU!grachev From: grachev@IMB.IMB.AC.RU (Alexei Grachev) Newsgroups: bionet.molecules.repertoires Subject: Ph.D. studentship or research assistant position wanted. Date: 8 Jan 1996 13:41:54 -0800 Organization: Institute of Molecular Biology Lines: 187 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601082139.AAA14202@imb.imb.ac.ru> NNTP-Posting-Host: net.bio.net Hello, Everybody, I am 23 y.o. junior scientist with MS in Biophysics, working in the Engelghardt Institute of Molecular Biology. I am looking for Ph.D. studentship abroad (preferably in Europe). Also any research position, (research assistant or something else), leading to Ph.D. in 3-5 years will be wonderful. I have been working in the field of Molecular Biology for 7 years, starting at the time when I was studying in secondary school. I continued my work in this field during my studying in Lomonosov Moscow State University. I performed my first scientific research at Institute of Carcinogenesis (Cancer Research Center, Moscow). My diploma project (5th-6th years) consisted of the study of Rous Sarcoma Virus (RSV) mRNA stability and transcriptional activity of RSV promoter in RSV- transformed hamster fibroblasts, also I was involved in some other projects (one of them - Molecular markers of cervical cancer). During my work I studied the Basic Molecular Biology Techniques such as molecular cloning, Southern, Northern blot hybridization, sequencing, the most common cell culture techniques, CAT assay, Luciferase assay, PCR. With best regards, Alexei Gratchev. P.S. Please find my CV and Official Transcripts enclosed. -CURRICULUM VITAE.- Name: Alexei Gratchev Personal: Born in February 23, 1972, Moscow, Russia (USSR). Education: 1989-1995: Student of Physical Department of Lomonosov Moscow State University ( MS in Biophysics, 1995) Mailing address: Moscow, 117419, PO Box 767, Russia. E-mail: grachev@imb.imb.ac.ru Professional employment: 1987-1989: Technichian, Institute of Carcinogenesis, Cancer Research Center, Moscow, Russia. 1989-II.1995: Research Technichian, Institute of Carcinogenesis, Cancer Research Center, Moscow, Russia. II.1995-Present: Research Technichian, Engelhardt Institute of Molecular Biology, Moscow, Russia. Research Experience: 1994-1995 Project title: Modulation of the expression of Rous Sarcoma Virus by N-ras oncogene (Supervisor: Dr. Natalja Kisseljova). List of Publuications: E.Samoylova, A.Gratchev, S.Petrov, G.Shaikhaiev. Consensus and type specific amplification in studying of HPV infection in women with cervical cancer in Russia. Book of Abstr. of 2nd Intern.Congress of Papillomavirus in Human Pathology, Paris, France, 112, 1994. References: Prof. Alexander G.Tatosyan, Head of the Laboratory of Tumour Transforming Genes. Cancer Research Center, Kashirskoye sh., 24, Moscow,115478, Russia. Tel: (095) 323-58-11 FAX: (095) 324-12-05 E-mail: oncogene@glas.apc.org Dr. Oleg A.Pavlish, Ph.D., Laboratory of Viral Carcinogenesis, Cancer Research Center, Kashirskoye sh., 24, Moscow,115478, Russia. Tel: (095) 323-57-55 FAX: (095) 324-12-05 Dr. Irina UDALOVA Malaria group, Department of Paediatrics, The Institute of Molecular Medicine, John Radcliffe Hospital, The University of Oxford, Oxford OX3 9DU tel.: 441-865-222-881 FAX : 441-865-222-626 E-mail: iudalova@molbiol.ox.ac.uk -Moscow State University namef after M.V.Lomonosov- -Physical Department- -Official transcript- Name: Alexei Gratchev Date of admission: 09/1/89 Graduate date: 02/28/95 Grade obtained: Masters degree in Physics Course name Hours Grade total obtained History of society and politics 100 5 Economical laws of society development 36 5 Ethics and aesthetics 18 passed Foreing language (English) 340 5 Philosophy 68 5 Global history and culture 68 5 Selected humanities 36 passed Structure and functioning of Economics 32 passed Physical training 416 passed Analytic geometry and linear algebra 102 5 Experimental techniques, safety and environmental protection 102 passed Differential and integral equations; variational calculus 112 5 History and methodology of physics 36 passed Quantum theory 170 5 Practical course (Molecular biology) 350 passed Calculus 328 5 Atomic physics 72 5 Mechanics 72 5 Molecular physics 96 5 Optics 88 5 Electricity 108 5 Nuclear physics 64 5 Practical training in general physics 364 passed Practical training in programming and computer maintance 18 passed Radio physics 44 5 Practical course: electronics and automatics 72 passed Automation of the experiment 36 passed Practical training in physics 402 passed Theory of probability and mathematical statistics 64 5 Complex variable functions 72 5 Thermodynamics and statistical physics 154 4 Methods of mathematical physics 126 5 Numerical methods in physics 36 4 Electrodynamics 136 5 Physics of fluids 36 4 Theoretical mechanics and basis of continua 152 5 courses of specialisation (Biophysics) 210 passed Industrial practice 19 weeks 5 Optical and magnetical properties of molecules 54 5 Biochemistry 54 5 Genegal biology 36 4 Physical chemistry 72 4 Physiology 32 5 Quantum chemistry 32 5 Physics of biological polymers 38 5 Diploma project Modulation of the expression of Rous sarcoma virus under the influence of N-ras oncogene 5 5-excellent; 4-good; 3-satisfactory; 2-bad; 1-very bad Translation is right Mrs. Valentina Tsitovich Assistant Director Engelghardt Institute of Molecular Biology Thank you for considering my application. From owner-repertoires@net.bio.net Mon Jan 08 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: Troels Wind Newsgroups: bionet.molecules.repertoires Subject: Leader sequence Date: 9 Jan 1996 17:45:17 -0000 Lines: 14 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4cu9jd$851@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk (molreps) Dear all, In most phagemid-systems I've seen, the leader-sequence of choice is the pelB. What is the reason for this? Is it the best, or is it just a habbit? Furthermore, can one be sure that the pelB peptide is cleaved off after translocation across the inner membrane? Thanks in advance, Troels Wind University of Aarhus Denmark From owner-repertoires@net.bio.net Tue Jan 09 22:00:00 1996 Path: biosci!BOTANY.UFL.EDU!jshao From: jshao@BOTANY.UFL.EDU Newsgroups: bionet.molecules.repertoires Subject: stringent elution of phage Date: 10 Jan 1996 07:35:53 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601101533.KAA18516@cutter.clas.ufl.edu> NNTP-Posting-Host: net.bio.net Hi, netter: Has any one tried to use more harsh eluent like Urea to elute displayed phage except Glycine at pH2 in the biopanning? Is renaturation of phage a problem here? If you have any experience, please e-mail me at: jshao @botany.ufl.edu Thanks. Jiahong Shao From owner-repertoires@net.bio.net Tue Jan 09 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: Andrew Bradbury Newsgroups: bionet.molecules.repertoires Subject: phage libraries etc Date: 10 Jan 1996 14:31:44 -0000 Lines: 2 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4d0ikg$1ab@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk Is this a news group I can subscribe to? andrew Bradbury From owner-repertoires@net.bio.net Wed Jan 10 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: "Andrew, Tel. +396-91093434" Newsgroups: bionet.molecules.repertoires Subject: Fair Shares Date: 11 Jan 1996 14:55:29 -0000 Lines: 33 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4d38d1$bua@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk Dear All, While it is great to see people posting questions to molreps for help with procedures, reagents, etc. (please keep doing this, it is one of the reasons molreps was created in the first place) please remember that the primary purpose of a newsgroup is the *sharing* of information. Therefore if you have a piece of helpful advice please post it to the newsgroup as well as to the person asking the question. To those asking questions - if you receive useful replies you should post a summary of them, not only to be helpful to others but also to help ensure that the newsgroup stays around for the future. I'd like to put together a FAQ list for this group so if anyone has any suggestions for topics and questions, useful ideas or wants to help with writing something please let me know. Andrew |========================================================================| | Andrew Wallace | Discussion on phage display, | | | combinatorial libraries, etc. - | | IRBM P. Angeletti, | bionet.molecules.repertoires | | Via Pontina KM 30.600 | molreps@daresbury.ac.uk | | 00040 Pomezia, Italy. |-------------------------------------------| | | "It has not escaped our notice that | | Voice: +39-6-91093434 | the specific pairing we have postulated | | Fax: +39-6-91093225 | immediately suggests a possible copying | | Email: wallace@irbm.it | mechanism for the genetic material." | | | | | DISCLAIMER: I do not speak | J.D. Watson and F.H.C. Crick | | on behalf of anyone. | in Nature 171:737-737 (1953). | |========================================================================| From owner-repertoires@net.bio.net Wed Jan 10 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: Troels Wind Newsgroups: bionet.molecules.repertoires Subject: Max. size of pIII fusion partner? Date: 11 Jan 1996 18:58:59 -0000 Lines: 13 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4d3mlj$2r6@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk (molreps) Hi again, Here's another question regarding phage display. There must oviously be a upper limimt to the size of the protein displayed on phage, even when fused to pIII in a monovalent manner. Can anyone guide me to a ref adressing this issue or contribute with their own experience, i.e. how large a molecule have YOU displayed? Thanks, Troels Wind Institute of Molecular and Structural Biology University of Aarhus Denmark From owner-repertoires@net.bio.net Sun Jan 14 22:00:00 1996 Path: biosci!agate!holonet!colossus.holonet.net!news2.net99.net!news.cais.net!primus.ac.net!news.internetMCI.com!newsfeed.internetmci.com!newsxfer2.itd.umich.edu!newsxfer.itd.umich.edu!news2.acs.oakland.edu!nntp.coast.net!news00.sunet.se!sunic!news99.sunet.se!columba.udac.uu.se!image.bmc.uu.se!user From: msz@bio.embnet.se (Michael Szardenings) Newsgroups: bionet.molecules.repertoires Subject: Re: Fair Shares Date: Mon, 15 Jan 1996 10:16:18 +0100 Organization: Uppsala University, BMC, Farm.Farm. Lines: 48 Distribution: bionet Message-ID: References: <4d38d1$bua@mserv1.dl.ac.uk> NNTP-Posting-Host: image.bmc.uu.se In article <4d38d1$bua@mserv1.dl.ac.uk>, "Andrew, Tel. +396-91093434" wrote: > Dear All, > > While it is great to see people posting questions to molreps for help with > procedures, reagents, etc. (please keep doing this, it is one of the > reasons molreps was created in the first place) please remember that the > primary purpose of a newsgroup is the *sharing* of information. Therefore if > you have a piece of helpful advice please post it to the newsgroup as well > as to the person asking the question. I just wanted to say, that I agree. I have been following this newsgroup, since it ever came into existence and the most useful thing were monthly literature updates. I just wonder where all those people are, that use 'repertoire' methods. Only very recently I have started to work myself wholehearted in this field, but I can not claim to have taken any advantage or to have received new interesting information by reading this group regularly. > To those asking questions - if you receive useful replies you should post a > summary of them, not only to be helpful to others but also to help ensure > that the newsgroup stays around for the future. To the only question, that was posted from myself to this list I got 0.0 replies. perhaps this is the same for other people. Perhaps this is also why this news group seems to me more or less dying. > I'd like to put together a FAQ list for this group so if anyone has any > suggestions for topics and questions, useful ideas or wants to help with > writing something please let me know. That would be GREAT. Regards to all out there in the silence Michael ========================================================================= * * ****** ******** ********************************************* ** ** *** ** ******** * Michael Szardenings * *** *** ***** *** * Biomedical Center, Pharm. Pharm. * **** **** ***** *** * Box 591 Tel.:int46-18-17422* ** *** ** ** *** ******** * S-751 24 Uppsala FAX :int46-18-55971* ** * ** ****** ******** ********************************************* ========================================================================= INTERNET : MSZ@bmc.uu.se ========================================================================= From owner-repertoires@net.bio.net Sun Jan 14 22:00:00 1996 Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!uwm.edu!msunews!harbinger.cc.monash.edu.au!lugb.latrobe.edu.au!newsmgr From: Mick Foley Newsgroups: bionet.molecules.repertoires Subject: (no subject) Date: 15 Jan 1996 02:08:47 GMT Organization: LaTrobe University Lines: 73 Message-ID: <4dccvf$s30@lugb.latrobe.edu.au> NNTP-Posting-Host: bcgf1.latrobe.edu.au Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Hi all, A few weeks ago I posted the following question: > We have been storing phage expressing a scFv on gene III at 4oC and find that after 4-5 days we get loss of binding to the antigen. Even after PEG precipitation. Can anyone suggest the best way to store them at 4oC so they remain useful for assays etc.< Thanks for all those who replied with useful stuff here is a summary of the replies: Henni Hoogenboom said: We store our phages in 15% glycerol at -70 (also for repertoires), which provides a stable source of phage as regard to infection titers. Also the display is maintained all right. Cinzia Trabni offered this advice we have experienced more Fab-phage than scFv-phage, but in both cases we store them at 4oC upon addition of 1 mM PMSF and 0,02% NaN3 and they are working for all the time we store them. A few days ago I have used a Fab-phage prepared in 1992! Anyway, it could be possible that a specific scFv is particularly unstable or that, if you reveal the binding through a tag, that the tag itself is easily degraded. Paolo Monacis’ experience was: We found that it is very important to prevent bacterial growth in the phage supernatant. To this end we carefully pellet bacteria, when long-term storage is needed we filter with 0.225 filters the sup, finally we add preservants (NaN3, Thi merosal) to avoid bacterial growth. In this way sups retain their binding proper ties at least for 3-4 weeks. Lastly Markus Renschler offered this advice: your observation is correct. We always grow them fresh for assays. The phage remains infectious for years at 4 degrees, thus it can always be used to reinfect fresh bacteria. We freeze the bacteria infected with each clone and usually keep purified phage at 4 degrees, but don't use them for assays. ------------------------------------------------------------------------- ------------- We have started to add NaN3 as suggested and found that the phage stay active in ELISA and dot blots for a few weeks. It does seem that there is no substitute for making them fresh if you want to squeeze every last OD unit out of your phage. In our experience using them as soluble scFv and visualising with an antibody to the peptide tag is better. They are simple to produce and are relatively stable for at least a few weeks. Thanks once again for the help. Cheers, Mick From owner-repertoires@net.bio.net Sun Jan 14 22:00:00 1996 Newsgroups: bionet.molecules.repertoires Path: biosci!fconvx.ncifcrf.gov!kaylor!toms From: toms@kaylor.ncifcrf.gov (Tom Schneider) Subject: Re: More on sequence logos Message-ID: Sender: usenet@ncifcrf.gov (C News) Nntp-Posting-Host: kaylor.ncifcrf.gov Organization: Frederick Biomedical Supercomputer Center X-Newsreader: TIN [version 1.2 PL2] Distribution: bionet Date: Mon, 15 Jan 1996 20:46:27 GMT Lines: 63 NOTE: We had a lot of trouble posting last fall so I don't think the following response ever got out. My posting was about a method for displaying the results of a "molecular repertoire" so that you don't throw away your data by making a "consensus sequence". The method is a graphical one called a "sequence logo". Tom | From: toms@kaylor.ncifcrf.gov (Tom Schneider) | Newsgroups: bionet.molecules.repertoires | Subject: Re: More on sequence logos | Date: Thu, 19 Oct 1995 04:02:26 GMT | Organization: Frederick Biomedical Supercomputer Center | Message-ID: Andrew Wallace writes: | Sounds interesting, Tom. Would you be prepared to post some of the Web page | material here so that those of us without access to a web browser can also | take a look? Unfortunately that's difficult because the sequence logos are a visual display. They are in PostScript and I have put some in GIF format for the web pages. If you have access to ftp (file transfer protocol) you can look into my archive at ftp.ncifcrf.gov in the directory pub/delila. Some example logos there are: SequenceLogoSculpture.ps (do this one first!) gallery.logo.ps globin.logo.ps hawaii.fig1.ps keller.ps lambcro.logo.ps lexa.logo.ps rbs1055.clist.ps rbs1055.logo.ps ribo.logo.ps splice.cover.ps t7.logo.ps papers: colonsplice.ps hawaii.ps (do this one first!) logopaper.ps (the original paper) malign.ps (multiple alignment) nano2.ps (review) philgen.ps (about databases) primer.ps (teaches info theory) secondlaw.ps sipblurb.ps trieste1995.ps (review) The set of papers we can send to people are listed in: cover.ps If you would like these, email me your physical address. Tom Schneider National Cancer Institute Laboratory of Mathematical Biology Frederick, Maryland 21702-1201 toms@ncifcrf.gov http://www-lmmb.ncifcrf.gov/~toms/ From owner-repertoires@net.bio.net Sun Jan 14 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: "Andrew, Tel. +396-91093434" Newsgroups: bionet.molecules.repertoires Subject: Conference announcement Date: 15 Jan 1996 15:57:57 -0000 Lines: 295 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4ddti5$sri@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk Forwarded from the MOL-DIVERSITY listserver. Note that I am not the original poster of this message so please direct all follow-up enquiries to the appropriate address below. Andrew This announcement has been published on several listservers, I apologize for any duplication or inconvenience. NOTE: A hardcopy of the Provisional Program and Registration Form for the conference will be mailed at the end of January to all authors who will present papers or posters at the conference and to those scientists who replied to the first circular indicating their interest. ------------------------------------------------------------------------------ PROVISIONAL PROGRAM AND REGISTRATION FORM FOURTH INTERNATIONAL CONFERENCE ON CHEMICAL STRUCTURES Sunday, June 2, through Thursday, June 6, 1996 Leeuwenhorst Congress Center Noordwijkerhout, The Netherlands Sponsors: --------- Chemical Structure Association (CSA) Chemistry-Information-Computer Division of the Society of German Chemists (GDCh) Division of Chemical Information of the American Chemical Society (ACS) New Swiss Chemical Society (NSCS) Royal Netherlands Chemical Society (KNCV) Royal Society of Chemistry, Chemical Information Group (RSC) The International Conference on Chemical Structures brings together an international group interested in handling chemical structures and related topics. Participants discuss research and development in the processing, storage, retrieval and use of chemical structures. The conference fosters cooperation among organizations and researchers involved with chemical structures and chemical information. Organizing Committee -------------------- Dr. Guenter Grethe, Chairman MDL Information Systems, Inc. USA Mrs. Janet E.Ash United Kingdom Dr. John M. Barnard BCI Ltd. United Kingdom Mr. John E. Blackmore Infonology Ltd. United Kingdom drs. Charles L. Citroen Delft University of Technology The Netherlands Dr. Vincent J. van Geerestein B. V. Organon The Netherlands Dr. Reiner Luckenbach Beilstein Institut Germany Dr. Jacques Weber Universite' de Gene've Switzerland Program ------- The conference will open with a keynote address on Sunday afternoon, June 2, and will continue until lunchtime on Thursday, June 6. The main technical program consists of five plenary sessions that include papers listed below. In addition there will be a new-product review session for commercial presentations, an extended poster session, and an exhibition featuring both commercially available software and services and also software from research projects. There will be ample time for informal discussions. The official conference language is English. Location and Accommodation -------------------------- The conference will take place at the Leeuwenhorst Congress Center, Noordwijkerhout, The Netherlands. This is a modern, comprehensive center, which is easily reached from Schiphol airport and readily accessible from major European cities by train or automobile. The Center, near Leiden and approximately equidistant from Amsterdam and Den Haag, is in a quiet rural setting only 2 km from the dunes and 4 km from the beach. There are a number of recreational facilities available, including tennis courts, an indoor swimming pool, and bicycle rentals. Accommodation at the Center includes single and twin-bedded rooms, each with its own shower and toilet, some have a bath. All rooms have telephones. Provisional Program ------------------- The keynote address on Sunday will be delivered by Prof. Dr. Juergen Brickmann, TH Darmstadt, Germany. Following is a list of papers that will be presented in the plenary sessions, additional papers might be selected by the Technical Program Committee. Over thirty posters will be presented in poster sessions Session A - Diversity Analysis ------------------------------ W. Douglas Hounshell, MDL Information Systems, Inc., "Algorithmic Classification of Structures by Functional Groups and Ring Systems". Robert D. Brown, Abbott Laboratories, "Investigating Molecular Diversity. Evaluating Descriptors and Clustering Methods". Peter Willett, University of Sheffield, "New Techniques for Dissimilarity-Based Compound Selection". Andrew Rusinko III, Glaxo Wellcome Inc., "Novel Uses of Graph Eigenvalues: Application to Database Mining and Combinatorial Chemistry". Daniel Domine, CTIS, "Optimal Test Series Design in Structure-Activity Relationship Studies". Johann Gasteiger, Universitaet Erlangen-Nuernberg, "Chemical Structures Are Three-Dimensional: The 3D-MoRSE Code". Session B - Combinatorial Chemistry ----------------------------------- Wendy A. Warr, Wendy Warr & Associates, "An Overview of Information Management Problems in Combinatorial Chemistry and an Evaluation of Some Solutions". Dieter Poppinger, Ciba Ltd., "CICLOPS - The Ciba Chemical Library Optimization System". Mark G. Bures, Abbott Laboratories, "Investigating Molecular Diversity of Combinatorial Libraries". Geoff M. Downs, Barnard Chemical Information Ltd., "Techniques for Generating Descriptive Fingerprints in Combinatorial Libraries". Session C - General Papers -------------------------- Pierre Benichou, Questel-Orbit, "Handling Genericity in Chemical Structures Using the Markush Darc Software". Jun Xu, Bio-Rad Laboratories, "GMA: A Generic Match Algorithm for Structural Homomorphism, Isomorphism, Maximal Common Sub-structure Match and Its Applications". Markus Wagener, Universitaet Erlangen-Nuernberg, "Determination of Maximum Common Three-Dimensional Substructures Using a Genetic Algorithm". Session D - 3D-Structure Handling --------------------------------- John H. van Drie, Upjohn Laboratories, "The Selectivity Principle and Its Use in Developing Queries for 3D Database Searching". Peter Willett, University of Sheffield, "Similarity Searching in Databases of 3-D Structures Using Molecular Field Information". Mathew A. Hahn, Biosym/Molecular Simulations, "Three-Dimensional Shape- Based Searching of Conformationally Flexible Compounds". Zsolt Zsoldos, University of Leeds, "Fast Ligand Docking from an Infinite 3D Database". Johanna M. Jansen, Uppsala University, "Drug Design Using the Minireceptor Concept". Session E - Reaction and Synthesis Planning ------------------------------------------- Engelbert Zass, ETH Zuerich, "Beilstein CrossFire Plus Reactions - A New Reaction Database in Comparison". Paul E. Blower, Chemical Abstracts Service, "Finding Reactions in a Large Database". Martin A. Ott, CAOS/CAMM Center, "Long-Range Strategies in the LHASA Program: The Quinone Diels Alder Transform". Rainer Moll, CASAF Gesellschaft fuer Computerchemie mbH, "Reaction Databases and Synthesis Planning: Combined Application and Synergistic Effects". Guido Sello, Universita' di Milano, "Organic Synthesis Planning: Some Hints from Similarity". For Information For more information about the conference, contact: Dr. John M. Barnard Barnard Chemical Information Ltd. 46 Uppergate Road Stannington Sheffield S6 6BX United Kingdom Tel:44 114 233 3170 Fax:44 114 234 3415 e-mail:barnard@bci1.demon.co.uk Dr. Vincent J. van Geerestein N. V. Organon P.O.Box 20 5340 BH Oss The Netherlands Tel:31 412 661 882 Fax:31 412 662 539 e-mail:v.geerestein@organon.akzonobel.nl Dr. Guenter Grethe MDL Information Systems, Inc. 14600 Catalina Street San Leandro, CA 94577 USA Tel:1 510 895 1313 Fax:1 510 614 3652 e-mail:guenter@mdli.com Dr. Reiner Luckenbach Beilstein Institut Varrentrappstrasse 40-42 D-60486 Frankfurt Germany Tel:49 69 7917 250 Fax:49 69 7917 511 e-mail:rluckenbach@beilstein.com For information about the exhibition contact: Mr. Harry Collier Infonortics Limited Tel.: 44 1249 814584 Fax.: 44 1249 813656 e-mail: 100142.2211@compuserve.com Registration ------------ Name (Dr/Mr/Mrs/Miss/Ms) ______________________________________ Organization___________________________________________________ Address________________________________________________________ ________________________________________________________ Tel.:___________________________Fax:___________________________ e-mail address:________________________________________________ Conference Fee (includes registration, proceedings, all meals, excursion, conference dinner) ---- Dfl. 1020 Dfl.______ Companion Fees (includes breakfasts, dinners, excursion, conference dinner) ---- Dfl. 500/per person Dfl.______ Name of Companion(s)__________________________________ ___________________________________ Room Fees (for four nights - Sunday through Thursday) Standard: single / with companion ---- Dfl. 360 / 660 Dfl._______ Large: single / with companion ---- Dfl. 560 / 980 Dfl._______ Extra nights accomodations are available at timely request from the local organizing committee but will have to be paid directly to the Conference Center on departure. Discounts Advance registration prior to March 1, 1996 (- Dfl. 100) Dfl._______ Advance student registration prior to March 1, 1996 (- Dfl.500) Dfl._______ Total Dfl._______ You will be invoiced for the amount due or you can fill in the credit card details below __American Express __VISA __MasterCard/Eurocard Your name as it appears on the card_____________________________________ Card Number_____________________________ Expiration Date______________ Signature___________________________________ Date___________________ Fax number for registrations : (+31) 412 662 539 --- Dr. Vincent J. van ---------------------------- Geerestein Bursaries --------- A limited number of bursaries are available for attending the conference. Student bursaries: The Chemical Structure Association Trust is offering bursaries to cover the conference and accomodation fees for up to three students Open bursaries: Up to L1000 will be awarded to assist attendees unable to meet their own conference expenses. These bursaries, also to be awarded by the Chemical Structure Association Trust, result from generous sponsorship by the ZENECA Group. All bursaries will be allocated on merit, and applications for a bursary should be made, together with a supporting reference, before 1st March 1996, to: Janet Ash, Secretary of Chemical Structure Association Trust Herengracht 14E 1015BK Amsterdam The Netherlands Tel/Fax: (+31) 20 6269610 e-mail: ash@euronet.nl From owner-repertoires@net.bio.net Sun Jan 14 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: "Andrew, Tel. +396-91093434" Newsgroups: bionet.molecules.repertoires Subject: Repertoirian of the Year Date: 15 Jan 1996 15:51:34 -0000 Lines: 26 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4ddt66$s2s@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk Dear All, Who do you think has made the greatest contribution to the field of molecular diversity / combinatorial chemistry during the past year? Is there someone you have in mind who would deserve the title Repertoirian of the Year 1995? Who would you propose? Send your suggestions to molreps@dl.ac.uk or molreps@net.bio.net and let's see who we can come up with! Andrew |========================================================================| | Andrew Wallace | Discussion on phage display, | | | combinatorial libraries, etc. - | | IRBM P. Angeletti, | bionet.molecules.repertoires | | Via Pontina KM 30.600 | molreps@daresbury.ac.uk | | 00040 Pomezia, Italy. |-------------------------------------------| | | "It has not escaped our notice that | | Voice: +39-6-91093434 | the specific pairing we have postulated | | Fax: +39-6-91093225 | immediately suggests a possible copying | | Email: wallace@irbm.it | mechanism for the genetic material." | | | | | DISCLAIMER: I do not speak | J.D. Watson and F.H.C. Crick | | on behalf of anyone. | in Nature 171:737-737 (1953). | |========================================================================| From owner-repertoires@net.bio.net Tue Jan 16 22:00:00 1996 Path: biosci!daresbury!yama.mcc.ac.uk!peer-news.britain.eu.net!psinntp!psinntp!psinntp!howland.reston.ans.net!vixen.cso.uiuc.edu!newsfeed.internetmci.com!newsserver.jvnc.net!synapse.bms.com!news-admin From: nathan@protos.bms.com (Nathan O. Siemers) Newsgroups: bionet.molecules.repertoires Subject: Re: Leader sequence Date: 16 Jan 1996 19:33:04 -0800 Organization: Bristol-Myers Squibb Lines: 29 Distribution: bionet Message-ID: <5120ozxzpr.fsf@protos.bms.com> References: <4cu9jd$851@mserv1.dl.ac.uk> NNTP-Posting-Host: protos.bms.com X-Newsreader: September Gnus v0.26/Emacs 19.29 >>> On 9 Jan 1996 17:45:17 -0000, Troels Wind said: TW> Dear all, TW> In most phagemid-systems I've seen, the leader-sequence of choice TW> is the pelB. What is the reason for this? Is it the best, or is it TW> just a habbit? As far as I know, no systematic study has shown that pelb is better or more versatile than other bacterial leader sequences. Perhaps others know more than I... TW> Furthermore, can one be sure that the pelB peptide is cleaved off TW> after translocation across the inner membrane? I would say that *if* it is translocated, the leader will be clipped. So if are you truly isolating a periplasmic fraction your protein should be all right. If you isolate from sups, you may have periplasmic material or possibly cytoplasmic protein (from cell lysis) with the leader still intact. TW> Thanks in advance, TW> Troels Wind University of Aarhus Denmark nathan From owner-repertoires@net.bio.net Thu Jan 18 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: "Andrew, Tel. +396-91093434" Newsgroups: bionet.molecules.repertoires Subject: Conference announcement Date: 19 Jan 1996 10:08:39 -0000 Lines: 298 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4dnqj7$i1s@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk *I am resending this message due to a network failure. Sorry for any *inconvenience. Forwarded from the MOL-DIVERSITY listserver. Note that I am not the original poster of this message so please direct all follow-up enquiries to the appropriate address below. Andrew This announcement has been published on several listservers, I apologize for any duplication or inconvenience. NOTE: A hardcopy of the Provisional Program and Registration Form for the conference will be mailed at the end of January to all authors who will present papers or posters at the conference and to those scientists who replied to the first circular indicating their interest. ------------------------------------------------------------------------------ PROVISIONAL PROGRAM AND REGISTRATION FORM FOURTH INTERNATIONAL CONFERENCE ON CHEMICAL STRUCTURES Sunday, June 2, through Thursday, June 6, 1996 Leeuwenhorst Congress Center Noordwijkerhout, The Netherlands Sponsors: --------- Chemical Structure Association (CSA) Chemistry-Information-Computer Division of the Society of German Chemists (GDCh) Division of Chemical Information of the American Chemical Society (ACS) New Swiss Chemical Society (NSCS) Royal Netherlands Chemical Society (KNCV) Royal Society of Chemistry, Chemical Information Group (RSC) The International Conference on Chemical Structures brings together an international group interested in handling chemical structures and related topics. Participants discuss research and development in the processing, storage, retrieval and use of chemical structures. The conference fosters cooperation among organizations and researchers involved with chemical structures and chemical information. Organizing Committee -------------------- Dr. Guenter Grethe, Chairman MDL Information Systems, Inc. USA Mrs. Janet E.Ash United Kingdom Dr. John M. Barnard BCI Ltd. United Kingdom Mr. John E. Blackmore Infonology Ltd. United Kingdom drs. Charles L. Citroen Delft University of Technology The Netherlands Dr. Vincent J. van Geerestein B. V. Organon The Netherlands Dr. Reiner Luckenbach Beilstein Institut Germany Dr. Jacques Weber Universite' de Gene've Switzerland Program ------- The conference will open with a keynote address on Sunday afternoon, June 2, and will continue until lunchtime on Thursday, June 6. The main technical program consists of five plenary sessions that include papers listed below. In addition there will be a new-product review session for commercial presentations, an extended poster session, and an exhibition featuring both commercially available software and services and also software from research projects. There will be ample time for informal discussions. The official conference language is English. Location and Accommodation -------------------------- The conference will take place at the Leeuwenhorst Congress Center, Noordwijkerhout, The Netherlands. This is a modern, comprehensive center, which is easily reached from Schiphol airport and readily accessible from major European cities by train or automobile. The Center, near Leiden and approximately equidistant from Amsterdam and Den Haag, is in a quiet rural setting only 2 km from the dunes and 4 km from the beach. There are a number of recreational facilities available, including tennis courts, an indoor swimming pool, and bicycle rentals. Accommodation at the Center includes single and twin-bedded rooms, each with its own shower and toilet, some have a bath. All rooms have telephones. Provisional Program ------------------- The keynote address on Sunday will be delivered by Prof. Dr. Juergen Brickmann, TH Darmstadt, Germany. Following is a list of papers that will be presented in the plenary sessions, additional papers might be selected by the Technical Program Committee. Over thirty posters will be presented in poster sessions Session A - Diversity Analysis ------------------------------ W. Douglas Hounshell, MDL Information Systems, Inc., "Algorithmic Classification of Structures by Functional Groups and Ring Systems". Robert D. Brown, Abbott Laboratories, "Investigating Molecular Diversity. Evaluating Descriptors and Clustering Methods". Peter Willett, University of Sheffield, "New Techniques for Dissimilarity-Based Compound Selection". Andrew Rusinko III, Glaxo Wellcome Inc., "Novel Uses of Graph Eigenvalues: Application to Database Mining and Combinatorial Chemistry". Daniel Domine, CTIS, "Optimal Test Series Design in Structure-Activity Relationship Studies". Johann Gasteiger, Universitaet Erlangen-Nuernberg, "Chemical Structures Are Three-Dimensional: The 3D-MoRSE Code". Session B - Combinatorial Chemistry ----------------------------------- Wendy A. Warr, Wendy Warr & Associates, "An Overview of Information Management Problems in Combinatorial Chemistry and an Evaluation of Some Solutions". Dieter Poppinger, Ciba Ltd., "CICLOPS - The Ciba Chemical Library Optimization System". Mark G. Bures, Abbott Laboratories, "Investigating Molecular Diversity of Combinatorial Libraries". Geoff M. Downs, Barnard Chemical Information Ltd., "Techniques for Generating Descriptive Fingerprints in Combinatorial Libraries". Session C - General Papers -------------------------- Pierre Benichou, Questel-Orbit, "Handling Genericity in Chemical Structures Using the Markush Darc Software". Jun Xu, Bio-Rad Laboratories, "GMA: A Generic Match Algorithm for Structural Homomorphism, Isomorphism, Maximal Common Sub-structure Match and Its Applications". Markus Wagener, Universitaet Erlangen-Nuernberg, "Determination of Maximum Common Three-Dimensional Substructures Using a Genetic Algorithm". Session D - 3D-Structure Handling --------------------------------- John H. van Drie, Upjohn Laboratories, "The Selectivity Principle and Its Use in Developing Queries for 3D Database Searching". Peter Willett, University of Sheffield, "Similarity Searching in Databases of 3-D Structures Using Molecular Field Information". Mathew A. Hahn, Biosym/Molecular Simulations, "Three-Dimensional Shape- Based Searching of Conformationally Flexible Compounds". Zsolt Zsoldos, University of Leeds, "Fast Ligand Docking from an Infinite 3D Database". Johanna M. Jansen, Uppsala University, "Drug Design Using the Minireceptor Concept". Session E - Reaction and Synthesis Planning ------------------------------------------- Engelbert Zass, ETH Zuerich, "Beilstein CrossFire Plus Reactions - A New Reaction Database in Comparison". Paul E. Blower, Chemical Abstracts Service, "Finding Reactions in a Large Database". Martin A. Ott, CAOS/CAMM Center, "Long-Range Strategies in the LHASA Program: The Quinone Diels Alder Transform". Rainer Moll, CASAF Gesellschaft fuer Computerchemie mbH, "Reaction Databases and Synthesis Planning: Combined Application and Synergistic Effects". Guido Sello, Universita' di Milano, "Organic Synthesis Planning: Some Hints from Similarity". For Information For more information about the conference, contact: Dr. John M. Barnard Barnard Chemical Information Ltd. 46 Uppergate Road Stannington Sheffield S6 6BX United Kingdom Tel:44 114 233 3170 Fax:44 114 234 3415 e-mail:barnard@bci1.demon.co.uk Dr. Vincent J. van Geerestein N. V. Organon P.O.Box 20 5340 BH Oss The Netherlands Tel:31 412 661 882 Fax:31 412 662 539 e-mail:v.geerestein@organon.akzonobel.nl Dr. Guenter Grethe MDL Information Systems, Inc. 14600 Catalina Street San Leandro, CA 94577 USA Tel:1 510 895 1313 Fax:1 510 614 3652 e-mail:guenter@mdli.com Dr. Reiner Luckenbach Beilstein Institut Varrentrappstrasse 40-42 D-60486 Frankfurt Germany Tel:49 69 7917 250 Fax:49 69 7917 511 e-mail:rluckenbach@beilstein.com For information about the exhibition contact: Mr. Harry Collier Infonortics Limited Tel.: 44 1249 814584 Fax.: 44 1249 813656 e-mail: 100142.2211@compuserve.com Registration ------------ Name (Dr/Mr/Mrs/Miss/Ms) ______________________________________ Organization___________________________________________________ Address________________________________________________________ ________________________________________________________ Tel.:___________________________Fax:___________________________ e-mail address:________________________________________________ Conference Fee (includes registration, proceedings, all meals, excursion, conference dinner) ---- Dfl. 1020 Dfl.______ Companion Fees (includes breakfasts, dinners, excursion, conference dinner) ---- Dfl. 500/per person Dfl.______ Name of Companion(s)__________________________________ ___________________________________ Room Fees (for four nights - Sunday through Thursday) Standard: single / with companion ---- Dfl. 360 / 660 Dfl._______ Large: single / with companion ---- Dfl. 560 / 980 Dfl._______ Extra nights accomodations are available at timely request from the local organizing committee but will have to be paid directly to the Conference Center on departure. Discounts Advance registration prior to March 1, 1996 (- Dfl. 100) Dfl._______ Advance student registration prior to March 1, 1996 (- Dfl.500) Dfl._______ Total Dfl._______ You will be invoiced for the amount due or you can fill in the credit card details below __American Express __VISA __MasterCard/Eurocard Your name as it appears on the card_____________________________________ Card Number_____________________________ Expiration Date______________ Signature___________________________________ Date___________________ Fax number for registrations : (+31) 412 662 539 --- Dr. Vincent J. van ---------------------------- Geerestein Bursaries --------- A limited number of bursaries are available for attending the conference. Student bursaries: The Chemical Structure Association Trust is offering bursaries to cover the conference and accomodation fees for up to three students Open bursaries: Up to L1000 will be awarded to assist attendees unable to meet their own conference expenses. These bursaries, also to be awarded by the Chemical Structure Association Trust, result from generous sponsorship by the ZENECA Group. All bursaries will be allocated on merit, and applications for a bursary should be made, together with a supporting reference, before 1st March 1996, to: Janet Ash, Secretary of Chemical Structure Association Trust Herengracht 14E 1015BK Amsterdam The Netherlands Tel/Fax: (+31) 20 6269610 e-mail: ash@euronet.nl From owner-repertoires@net.bio.net Thu Jan 18 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: "Andrew, Tel. +396-91093434" Newsgroups: bionet.molecules.repertoires Subject: Repertoirian of the Year Date: 19 Jan 1996 10:07:08 -0000 Lines: 29 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4dnqgc$i05@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk *I am resending this message due to a network failure. Sorry for any *inconvenience. Dear All, Who do you think has made the greatest contribution to the field of molecular diversity / combinatorial chemistry during the past year? Is there someone you have in mind who would deserve the title Repertoirian of the Year 1995? Who would you propose? Send your suggestions to molreps@dl.ac.uk or molreps@net.bio.net and let's see who we can come up with! Andrew |========================================================================| | Andrew Wallace | Discussion on phage display, | | | combinatorial libraries, etc. - | | IRBM P. Angeletti, | bionet.molecules.repertoires | | Via Pontina KM 30.600 | molreps@daresbury.ac.uk | | 00040 Pomezia, Italy. |-------------------------------------------| | | "It has not escaped our notice that | | Voice: +39-6-91093434 | the specific pairing we have postulated | | Fax: +39-6-91093225 | immediately suggests a possible copying | | Email: wallace@irbm.it | mechanism for the genetic material." | | | | | DISCLAIMER: I do not speak | J.D. Watson and F.H.C. Crick | | on behalf of anyone. | in Nature 171:737-737 (1953). | |========================================================================| From owner-repertoires@net.bio.net Thu Jan 18 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: "Andrew, Tel. +396-91093434" Newsgroups: bionet.molecules.repertoires Subject: Forwarded Conference Announcement Date: 19 Jan 1996 11:04:54 -0000 Lines: 29 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4dntsm$m8q@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk Forwarded from bionet.molbio.proteins Msg # 6279 This year's international Perspectives on Protein Engineering Conference is being held in Montpellier, South of France 2-6 March 1997 The overall theme is "Folds to Function" Sub-themes include: Molecular Bioinformatics (gene function discovery); protein evolution and design; protein folding; biocatalysis (enzyme engineering) and protein-nucleic acid interactions. A large World Wide Web site holds all the conference information, the scientific abstract and an on-line registration facility for the conference. Web site at - http://www.cryst.bbk.ac.uk/CEC/pope5.htm The European Commission has sponsored many groups funded under Framework III (Protein Structure and Function) to come to the conference. The result is that 5 "EU networks" are holding satellite minisymposia: Protein Translation Factors (Brian Clark, Aarhus) Cellulase Structure and Function (Richard Haser, Marseille) Glutamate receptors (Joel Bockaert/Jean Rossier, Montpellier/Gif-sur-Yvette) ScatterFactor/Human Growth Factor (Ermanno Gherardi, Cambridge) "Plantibodies - endowing plants with new functions (Arjen Schots, Wageningen) All these network programmes http://www.cryst.bbk.ac.uk/CEC/networks.html -- PERSPECTIVES SECRETARIAT POPE CONFERENCE Unit 5 The Hillside Center Upper Green ST. High Wycombe HP11 2RB, UK Fax +44 1 494 446672 From owner-repertoires@net.bio.net Thu Jan 18 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: "Andrew, Tel. +396-91093434" Newsgroups: bionet.molecules.repertoires Subject: PCR primers database available via web Date: 19 Jan 1996 10:10:56 -0000 Lines: 172 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4dnqng$i52@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk * Resending due to network failure - sorry for any inconvenience. Forwarded announcement from the EBI, Cambridge, UK Andrew ------------------------------------------------------------------------ Dear Colleague, I would like to draw your attention to a new database which is now being established. This is the PCR primers database. The database is based on submissions made by colleagues who perform PCR experiments. It holds information on tested, working sets of primers. The information should enable researchers to synthesize primers and follow the original experimental design, thus avoiding the hassle and high cost which is often associated with designing of new primers. Your Email address has been extracted from Usenet news articles. If this message was inadvertently sent to you, please excuse me for the trouble and inconvenience that it caused. This letter is an open invitation for you or any of your colleagues to visit the database WWW site (or alternatively use ftp or our email server) check the existing documentation and information and add your "public" tested and working primer sets to the database. Such a contribution may later save someone else much work. Currently, there's no possibility to feed the database by journal scanning, so the database relies ONLY on the good will of the researchers community. The database home page can be accessed through EBI's home page at: http://www.ebi.ac.uk/ in the databases area, or directly at: http://www.ebi.ac.uk/primers_home.html The data and documentation can be obtained through anonymous ftp to: ftp.ebi.ac.uk under: /pub/databases/primers/ Users who have no other means of connection besides email, can now use the EMBL-EBI automatic mail server to retrieve the database files and documents. To start working with the mail server in general, send a message containing the word "HELP" either in the subject line or in the body of message, to: netserv@ebi.ac.uk Here are the commands which are available for the primers database: Here is a summary of all the available commands for the primers database, that can be specified in an email message sent to netserv@ebi.ac.uk ------------------------------------------------------------------------- To obtain send the command ------------------------------------------------------------------------- A general help file HELP PRIMERS The database fields definitions file GET PRIMERS:DEFINITIONS A bogus filled entry as an example GET PRIMERS:EXAMPLE An electronic data submission form GET PRIMERS:SUBMISSION.FORM The actual data file with the entries GET PRIMERS:PRIMERS ------------------------------------------------------------------------- Dear colleague, please do not forget, this database will NOT be created by journal scanning and active searching, but from direct submissions from researchers only. The key to the success of this database is your submissions, so do set aside some spare time and send your primers. Our email: primers@ebi.ac.uk WWW : http://www.ebi.ac.uk/primers_home.html fax : +44 1223 494468 Phone : +44 1223 494437 I highly welcome any questions, corrections, remarks or discussions concerning the PCR Primers database (or the British weather or any other topic... :). Email is the most preferred way. Best wishes. Good luck with your PCR experiments. Yours, Benny. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Benny Shomer External Biological Liaison Officer, EMBL outstation - The EBI, Hinxton Hall, Hinxton,Cambridge CB10 1RQ, UK Tel: +44-223-494437 Fax: +44-223-494468 Email: bshomer@EBI.ac.uk http://www.ebi.ac.uk/ebi_docs/staff/benny.html ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From owner-repertoires@net.bio.net Thu Jan 18 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: "Andrew, Tel. +396-91093434" Newsgroups: bionet.molecules.repertoires Subject: List of World Wide Web (www) sites Date: 19 Jan 1996 10:09:44 -0000 Lines: 41 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4dnql8$i31@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk *Resending due to network failure - sorry for any inconvenience. Dear Molecular Librarians, Here is the latest list of web sites of interest to repertoirians: http://www.bio.net/ - the BIOSCI web site itself (MOLREPS message archive) http://bionmr1.rug.ac.be/chemistry/overview.html - a useful chemistry site http://vesta.pd.com/ - site for the journal MOLECULAR DIVERSITY http://www.mrc-cpe.cam.ac.uk/imt-doc/vbase-home-page.html - immunoglobulin v-gene database Molecular biologists desk reference< Keep those sites coming in! Regards, Andrew |========================================================================| | Andrew Wallace | Discussion on phage display, | | | combinatorial libraries, etc. - | | IRBM P. Angeletti, | | | Via Pontina KM 30.600 | molreps@irbm.it | | 00040 Pomezia, Italy. |-------------------------------------------| | | "It has not escaped our notice that | | Voice: +39-6-91093434 | the specific pairing we have postulated | | Fax: +39-6-91093225 | immediately suggests a possible copying | | Email: wallace@irbm.it | mechanism for the genetic material." | | | | | DISCLAIMER: I do not speak | J.D. Watson and F.H.C. Crick | | on behalf of anyone. | in Nature 171:737-737 (1953). | |========================================================================| From owner-repertoires@net.bio.net Thu Jan 18 22:00:00 1996 Path: biosci!irbm.it!wallace From: wallace@irbm.it (Andrew Wallace) Newsgroups: bionet.molecules.repertoires Subject: Thanks to Mick and the others Date: 19 Jan 1996 11:19:07 -0800 Organization: IRBM Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: <9601191146.AA08398@a2.irbm.it> NNTP-Posting-Host: net.bio.net Mick, thanks for posting this list of responses. I hope we can see more of this kind of activity, otherwise the group will die out. Actually I was lucky to see this message - I only found it by netsurfing to biosci's site at http://www.bio.net/ otherwise I would never have known it was there. Is anyone else having so much trouble with their mail recently? Maybe this is one reason why there's not much activity on this group - the messages are not getting out! Andrew |======================================================================| | Andrew Wallace | Discussion on phage display, | | | combinatorial libraries, etc. - | | IRBM P. Angeletti, | bionet.molecules.repertoires | | Via Pontina KM 30.600 | molreps@daresbury.ac.uk | | 00040 Pomezia, Italy. |-----------------------------------------| | | "It has not escaped our notice that | | Voice: +39-6-91093434 | the specific pairing we have postulated | | Fax: +39-6-91093225 | immediately suggests a possible copying | | Email: wallace@irbm.it | mechanism for the genetic material." | | | | | DISCLAIMER: I do not speak | J.D. Watson and F.H.C. Crick | | on behalf of anyone. | in Nature 171:737-737 (1953). | |======================================================================| From owner-repertoires@net.bio.net Sun Jan 21 22:00:00 1996 Path: biosci!pwinet.upj.com!hdfische From: hdfische@pwinet.upj.com (Dave Fischer) Newsgroups: bionet.molecules.repertoires Subject: new web site Date: 22 Jan 1996 05:40:16 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601221338.IAA12620@fatty.law.cornell.edu> NNTP-Posting-Host: net.bio.net Douglas Youvan's new web site at Kairos Scientific is now available and includes topics on combinatorial libraries, molecular repertoires, etc. The URL is http://www.Kairos-scientific.com At a recent conference on Applied Molecular Evolution Dr. Youvan invited all those with related web sites and interests to submit links for him to include from this site. From owner-repertoires@net.bio.net Wed Jan 24 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Forwarded mail from Andrew Bradbury Date: 25 Jan 1996 13:13:49 -0000 Lines: 22 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4e7vmd$dme@mserv1.dl.ac.uk> X-Vmsmail-Cc: WALLACE Original-To: bagni@A1.IRBM.IT, barbato@A1.IRBM.IT, bazzo@A1.IRBM.IT, bianchi@A1.IRBM.IT, clench@A1.IRBM.IT, cortese@A1.IRBM.IT, defrancesco@A1.IRBM.IT, delmastro@A1.IRBM.IT, esposito@A1.IRBM.IT, fattorid@A1.IRBM.IT, felici@A1.IRBM.IT Does anyone know of a tag (apart from myc) which has the following characteristics: hybridoma is available (i.e. you can grow your own) active at N and C terminal, and also within the middle of a protein small no background if used as a tag in transfected mammalian cells can be used to purify proteins I would like to use this to make a phage antibody library with a non-myc tag thanks to all andrew bradbury Andrew Bradbury SISSA c/o ICGEB Area di Ricerca Padriciano 99 Trieste 34012 Italy Tel +39 40 398995 Fax +39 40 398991 From owner-repertoires@net.bio.net Wed Jan 24 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Forwarded from bionet.proteins - another possible FAQ? Date: 25 Jan 1996 13:22:39 -0000 Lines: 12 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4e806v$edp@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk Does anyone have or know of any commercially available antibodies to filamentous phage minor coat protein pIII? I am looking for something that is specific and can be used in a Western blot. I am aware that there are commercially available rabbit anti-M13 from Pharmacia and Stratagene but would prefer something a bit more specific. Any help or suggestions are welcome. Thanks, Lyle Najita Plant Pathology University of California - Davis From owner-repertoires@net.bio.net Thu Jan 25 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Forwarded reply to pIII antibodies question Date: 26 Jan 1996 14:20:15 -0000 Lines: 33 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4eanuv$1od@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk, proteins@dl.ac.uk From: bradbury@icgeb.trieste.it (Andrew Bradbury) Subject: Re: Forwarded from bionet.proteins - another possible FAQ? anti M13 gp3 antibodies are provided (polyclonal) by GATC, a German company. These are very good (we've used them) and are diluted 1/10000 for western blots. The company can be reached on Fax +49 7531 57313. or telephone 7531 57209. andrew bradbury >Does anyone have or know of any commercially available antibodies to >filamentous phage minor coat protein pIII? I am looking for something >that is specific and can be used in a Western blot. I am aware that there >are commercially available rabbit anti-M13 from Pharmacia and Stratagene >but would prefer something a bit more specific. Any help or suggestions >are welcome. > >Thanks, > >Lyle Najita >Plant Pathology >University of California - Davis Andrew Bradbury SISSA c/o ICGEB Area di Ricerca Padriciano 99 Trieste 34012 Italy Tel +39 40 398995 Fax +39 40 398991 From owner-repertoires@net.bio.net Thu Jan 25 22:00:00 1996 Path: biosci!bloom-beacon.mit.edu!newsserver.pixel.kodak.com!news.sprintlink.net!rebecca!newserve!ub!dsinc!netnews.upenn.edu!msunews!uwm.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!newsfeed.internetmci.com!in2.uu.net!news00.sunet.se!sunic!news99.sunet.se!news.uni-c.dk!wind From: wind@biobase.dk (Troels Wind) Newsgroups: bionet.molecules.repertoires Subject: Anti pIII mAb Date: 25 Jan 1996 14:53:14 GMT Organization: News Server at UNI-C, Danish Computing Centre for Research and Education. Lines: 16 Message-ID: <4e85gq$e32@news.uni-c.dk> NNTP-Posting-Host: biobase.dk X-Newsreader: TIN [version 1.2 PL2] Hi all, Andrew forwarded a question about the availability of monoclonals towards pIII of filamentous phage. Bhardwaj et al. published the making of such mAbs in Journal of Immunological Methods (1995), 179 pp 165-175, but I tried writing the autors months ago to request samples or hybridomas, without luck. They never answered :-( I know that someone in germany has made mAbs against pIII, but unfortunately I cant remember who. Can any of you? Regards, Troels Wind University of Aarhus Denmark From owner-repertoires@net.bio.net Sat Jan 27 22:00:00 1996 Path: biosci!DARTMOUTH.EDU!Stefano.Liparoto From: Stefano.Liparoto@DARTMOUTH.EDU (Stefano Francesco Liparoto) Newsgroups: bionet.molecules.repertoires Subject: anti-p3 mAb Date: 28 Jan 1996 13:40:31 -0800 Organization: Dartmouth Medical School Lines: 30 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601282138.QAA30240@dartvax.dartmouth.edu> NNTP-Posting-Host: net.bio.net http://www.bio.net/hypermail/MOLECULAR-REPERTOIRES/9601/0025.html I know that someone in germany has made mAbs against pIII, but unfortunately I cant remember who. Can any of you? Troels Wind University of Aarhus De In 'Functional Display and Expression of Chicken Cystatin Using a Phagemid System' vol.214,no. 2, 1995 pages 389-395 "Biochemical and Biophysical Research Communications, the authors acknowledged Prof. Dr. John Collins and Peter Rottgen, Gesellschaft fur Biologische Forschung, Braunschweig for providing mAb anti-gp3. This may be a source for some of you. Stefano Francesco Liparoto Dartmouth Medical School Dept. Pharmacology/Toxicology HB7650 Hanover,N.H. 03755-3835 USA From owner-repertoires@net.bio.net Sat Jan 27 22:00:00 1996 Path: biosci!DARTMOUTH.EDU!Stefano.Liparoto From: Stefano.Liparoto@DARTMOUTH.EDU (Stefano Francesco Liparoto) Newsgroups: bionet.molecules.repertoires Subject: (no subject) Date: 28 Jan 1996 10:33:02 -0800 Organization: Dartmouth Medical School Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601281830.NAA06093@dartvax.dartmouth.edu> NNTP-Posting-Host: net.bio.net Andrew forwarded a question about the availability of monoclonals towards pIII of filamentous phage. Bhardwaj et al. published the making of such mAbs in Journal of Immunological Methods (1995), 179 pp 165-175, but I tried writing the autors months ago to request samples or hybridomas, without luck. They never answered :-( I know that someone in germany has made mAbs against pIII, but unfortunately I cant remember who. Can any of you? Troels Wind University of Aarhus De In 'Functional Display and Expression of Chicken Cystatin Using a Phagemid System' vol.214,no. 2, 1995 pages 389-395, the authors acknowledged Prof. Dr. John Collins and Peter Rottgen, Gesellschaft fur Biologische Forschung, Braunschweig for providing mAb anti-gp3. This may be a source for some of you. From owner-repertoires@net.bio.net Sat Jan 27 22:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molecules.repertoires Subject: BIOSCI miniFAQ, ver. 14-DEC-95 Date: 28 Jan 1996 02:00:24 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 199 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601281000.CAA13620@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 14-DEC-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. Contents: -------- 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index in addition to the master index for the entire set. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-repertoires@net.bio.net Sun Jan 28 22:00:00 1996 Path: biosci!daresbury!lyra.csx.cam.ac.uk!news From: Peter Wang Newsgroups: bionet.molecules.repertoires Subject: Re: anti-gpIII MAb - reference Date: 29 Jan 1996 04:02:48 GMT Organization: Centre for Protein Engineering Lines: 38 Message-ID: <4ehgt8$oru@lyra.csx.cam.ac.uk> References: <199601281830.NAA06093@dartvax.dartmouth.edu> NNTP-Posting-Host: macx45.mrc-lmb.cam.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) X-URL: news:199601281830.NAA06093@dartvax.dartmouth.edu Stefano.Liparoto@DARTMOUTH.EDU (Stefano Francesco Liparoto) wrote: >Troels Wind (wind@biobase.dk) wrote: >>I know that someone in germany has made mAbs against pIII, but >>unfortunately >>I cant remember who. Can any of you? > > > In 'Functional Display and Expression of Chicken Cystatin Using a >Phagemid System' vol.214,no. 2, 1995 pages 389-395, the authors >acknowledged Prof. Dr. John Collins and Peter Rottgen, Gesellschaft fur >Biologische Forschung, Braunschweig for providing mAb anti-gp3. > The original reference for the anti-gpIII MAb is: TI: MONOCLONAL-ANTIBODY AGAINST PIII OF FILAMENTOUS PHAGE - AN IMMUNOLOGICAL TOOL TO STUDY PIII FUSION PROTEIN EXPRESSION IN PHAGE DISPLAY SYSTEMS AU: TESAR_M, BECKMANN_C, ROTTGEN_P, HAASE_B, FAUDE_U, TIMMIS_KN NA: GBF NAT RES CTR BIOTECHNOL,DEPT MICROBIOL,D-38124 BRAUNSCHWEIG,GERMANY GBF NAT RES CTR BIOTECHNOL,DEPT APPL GENET,D-38124 BRAUNSCHWEIG,GERMANY JN: IMMUNOTECHNOLOGY, 1995, Vol.1, No.1, pp.53-64 I saw a poster presentation by one of the authors; she seemed to be willing to send the MAb to people. Cheers, - Peter --------------------------------------------------------- Peter Wang, M.D., Ph.D. MRC Centre for Protein Engineering, Hills Road, Cambridge, CB2 2QH, England Tel (01223) 402104 (international calls +44-1223-402104) Fax (01223) 402140 ( " " +44-1223-402140) --------------------------------------------------------- From owner-repertoires@net.bio.net Mon Jan 29 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: List of World Wide Web (WWW) Sites Date: 30 Jan 1996 19:04:01 -0000 Lines: 42 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4elq31$io0@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk Dear Molecular Librarians, Here is the latest list of web sites of interest to repertoirians: http://www.bio.net/ - the BIOSCI web site itself (MOLREPS message archive) http://bionmr1.rug.ac.be/chemistry/overview.html - a useful chemistry site http://vesta.pd.com/ - site for the journal MOLECULAR DIVERSITY http://www.mrc-cpe.cam.ac.uk/imt-doc/vbase-home-page.html - immunoglobulin v-gene database http://molbio.info.nih.gov/molbio/desk.html - Molecular biologists desk reference *NEW* http://www.Kairos-scientific.com - Kairos scientific home page Keep those sites coming in! Regards, Andrew |========================================================================| | Andrew Wallace | Discussion on phage display, | | | combinatorial libraries, etc. - | | IRBM P. Angeletti, | bionet.molecules.repertoires | | Via Pontina KM 30.600 | molreps@dl.ac.uk | | 00040 Pomezia, Italy. |-------------------------------------------| | | "It has not escaped our notice that | | Voice: +39-6-91093434 | the specific pairing we have postulated | | Fax: +39-6-91093225 | immediately suggests a possible copying | | Email: wallace@irbm.it | mechanism for the genetic material." | | | | | DISCLAIMER: I do not speak | J.D. Watson and F.H.C. Crick | | on behalf of anyone. | in Nature 171:737-737 (1953). | |========================================================================| From owner-repertoires@net.bio.net Mon Jan 29 22:00:00 1996 Path: biosci!agate!usenet.kornet.nm.kr!xpat.postech.ac.kr!news.kreonet.re.kr!news.dacom.co.kr!nntp.coast.net!harbinger.cc.monash.edu.au!lugb.latrobe.edu.au!newsmgr From: Mick Foley Newsgroups: bionet.molecules.repertoires Subject: Re: Anti pIII mAb Date: 30 Jan 1996 07:49:13 GMT Organization: LaTrobe University Lines: 13 Message-ID: <4ekihp$muq@lugb.latrobe.edu.au> References: <4e85gq$e32@news.uni-c.dk> NNTP-Posting-Host: bcgf1.latrobe.edu.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Hi all, I got a monclonal antibody against gpII from Michael Tesar and it works beautifully on western blots. He is at the Deot of Microbiology Gesellschaft fur Biotechn., Forschung, Braunscweig 381124, Germany. His e-mail is a bit simpler it is: mte@venus.gbf-braunschweig.d400.de Hope this is of some use. Cheers, Mick From owner-repertoires@net.bio.net Mon Jan 29 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: List of World Wide Web (WWW) Sites Date: 30 Jan 1996 19:18:53 -0000 Lines: 45 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4elqut$jv4@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk Dear Molecular Librarians, Here is the latest list of web sites of interest to repertoirians: http://www.bio.net/ - the BIOSCI web site itself (MOLREPS message archive) http://bionmr1.rug.ac.be/chemistry/overview.html - a useful chemistry site http://vesta.pd.com/ - site for the journal MOLECULAR DIVERSITY http://www.mrc-cpe.cam.ac.uk/imt-doc/vbase-home-page.html - immunoglobulin v-gene database http://molbio.info.nih.gov/molbio/desk.html - Molecular biologists desk reference *NEW* http://www.Kairos-scientific.com - Kairos scientific home page *NEW* http://www-lmmb.ncifcrf.gov/~toms/ - Tom Schneider's Theory of Molecular Machines Keep those sites coming in! Regards, Andrew |========================================================================| | Andrew Wallace | Discussion on phage display, | | | combinatorial libraries, etc. - | | IRBM P. Angeletti, | bionet.molecules.repertoires | | Via Pontina KM 30.600 | molreps@daresbury.ac.uk | | 00040 Pomezia, Italy. |-------------------------------------------| | | "It has not escaped our notice that | | Voice: +39-6-91093434 | the specific pairing we have postulated | | Fax: +39-6-91093225 | immediately suggests a possible copying | | Email: wallace@irbm.it | mechanism for the genetic material." | | | | | DISCLAIMER: I do not speak | J.D. Watson and F.H.C. Crick | | on behalf of anyone. | in Nature 171:737-737 (1953). | |========================================================================|