From owner-repertoires@net.bio.net Sun Mar 03 22:00:00 1996 Path: biosci!rutgers!csn!news-1.csn.net!ub!dsinc!netnews.upenn.edu!msunews!caen!newsxfer2.itd.umich.edu!newsfeed.internetmci.com!newsserver.jvnc.net!news.cyanamid.com!news From: silvermans@pt.cyanamid.com Newsgroups: bionet.molecules.repertoires Subject: Re: alignment program? Date: 4 Mar 1996 20:33:04 GMT Organization: American Cyanamid Lines: 8 Message-ID: <4hfk20$n6h@igate2.pt.cyanamid.com> References: <199602291554.AA02870@gate.mmd.com> NNTP-Posting-Host: 141.173.54.39 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.22 (Windows; I; 16bit) The Intelligenetics Suite (used to) have the option of choosing a "type" of similarity in protein alignments. I think the one you want is Jimenez-Montano, if I recall correctly. I haven't used this in years so you would have to contact them or a recent user. Good luck. -------Sandy From owner-repertoires@net.bio.net Mon Mar 04 22:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!news.vub.ac.be!news.belnet.be!news.rediris.es!scsing.switch.ch!news.belwue.de!fu-berlin.de!cs.tu-berlin.de!uni-erlangen.de!lrz-muenchen.de!bs_boris.lmb.uni-muenchen.de!user From: steipe@lmb.uni-muenchen.de (Boris Steipe) Newsgroups: bionet.molecules.repertoires Subject: Ph.D. Yeast Interaction Trap, Munich, Germany Date: Tue, 05 Mar 1996 20:53:53 +0100 Organization: Genzentrum, biostructure lab Lines: 48 Distribution: world Message-ID: NNTP-Posting-Host: bs_boris.lmb.uni-muenchen.de X-Newsreader: Value-Added NewsWatcher 2.0b22.0+ Yeast Interaction Trap Techniques for Protein Engineering and Design ----------------------------------- The Biostructure Lab of the University of Munich, Genzentrum, has a research studentship available (BAT IIa/2). All biochemical processes originate from principles of molecular recognition. We are interested in rational protein engineering and in vitro evolution methods to engineer and design protein- protein interactions, based on the creation and modulation of molecular recognition principles. We have begun a project to establish a novel in vivo screening system, based on yeast interaction trap or two-hybrid system technology, and to opti- mize this for cell biology and molecular pharmacology. We work with a broad spectrum of methods, including molecular and cellular biology, protein chemistry, structural analysis and biophysics. If you are interested to work in modern, applications oriented, basic research and have some experience in molecular biology AND YEAST GENETICS, you should contact me as soon as possible. This position has no teaching requirements. German is not required. For further information, you can contact me (informally, via e-mail) using the address given below. Boris Steipe, March 4. 1996 +---Dr. Boris Steipe---------------+ | Genzentrum | | Wuermtalstr. 221 Tel.: +49 (89) 74017 - 417 | | 81375 Muenchen, Germany Fax.: - 448 | +------+ -- Boris From owner-repertoires@net.bio.net Mon Mar 04 22:00:00 1996 Path: biosci!biosci!not-for-mail From: steipe@lmb.uni-muenchen.de (Boris Steipe) Newsgroups: bionet.molecules.repertoires,bionet.molbio.yeast Subject: Ph.D.- Yeast Interaction Trap, Munich, Germany Date: 4 Mar 1996 21:43:15 -0800 Organization: Genzentrum, biostructure lab Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Xref: biosci bionet.molecules.repertoires:109 bionet.molbio.yeast:4878 Yeast INTERACTION TRAP Techniques for Protein Engineering and Design ------------------------ The Biostructure Lab of the University of Munich, GenZentrum, has a research studentship position available (BatIIA/2). All biochemical processes are based on principles of molecular recognition. We are interested in rational Protein Engineering and Design, to engineer and modulate molecular recognition in protein-protein interactions. For this, we have begun a project to establish a novel in vivo method, based on the Interaction Trap, for the detection of protein - protein interactions. This system is to be established and optimized for use in cell biology and molecular pharmacology. We work with a broad spectrum of methods: molecular and cellular biology, protein chemistry, structural analysis and biophysics. If you are interested in modern, applications oriented, basic research, experienced in molecular biology AND YEAST GENETICS, please contact me as soon as possible. Potential candidates can obtain further information informally by contacting me via e-mail. The position does not involve teaching duties. A knowledge of the German language is not essential. Boris Steipe, March 4. 1996 +---Dr. Boris Steipe---------------+ | Genzentrum | | Wuermtalstr. 221 Tel.: +49 (89) 74017 - 417 | | 81375 Muenchen, Germany Fax.: - 448 | +------+ -- Boris From owner-repertoires@net.bio.net Mon Mar 11 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: "Janet Clench, Library, Tel:(39 6)91093220" Newsgroups: bionet.molecules.repertoires Subject: And here we are, finally back again with 2 batches. Ciao Date: 11 Mar 1996 13:42:38 -0000 Lines: 170 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4i1ake$ab6@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk ******************************************************** Subject: Combinatorial & Phage Libraries Date: 12 & 19 February, 1996 ******************************************************** Collection of Czechoslovak Chemical Communications 60: 12 (DEC 1995) A Kasal, L Kohout, M Lebl Synthesis of a scaffold for the creation of non-peptide libraries 2147-2155 Journal of the American Chemical Society 118: 1 (JAN 10 1996) B Ruhland, A Bhandari, EM Gordon, MA Gallop Solid-supported combinatorial synthesis of structurally diverse beta-lactams 253-254 Journal of the American Chemical Society 118: 1 (JAN 10 1996) AP Combs, TM Kapoor, SB Feng, JK Chen, LF Daudesnow, SL Schreiber Protein structure-based combinatorial chemistry: Discovery of non-peptide binding elements to Src SH3 domain 287-288 Analytical Chemistry 68: 2 (JAN 15 1996) CL Brummel, JC Vickerman, SA Carr, ME Hemling, GD Roberts, W Johnson, J Weinstock, D Gaitanopoulos, SJ Benkovic, N Winograd Evaluation of mass spectrometric methods applicable to the direct analysis of non-peptide bead-bound combinatorial libraries 237-242 Angewandte Chemie - International Edition in English 34: 23-24 (JAN 5 1996) J Sadowski, M Wagener, J Gasteiger Assessing similarity and diversity of combinatorial libraries by spatial autocorrelation functions and neural networks 2674-2677 Angewandte Chemie - International Edition in English 34: 23-24 (JAN 5 1996) HP Wessel, CM Mitchell, CM Lobato, G Schmid Saccharide-peptide hybrids as novel oligosaccharide mimetics 2712-2713 Angewandte Chemie - International Edition in English 34: 23-24 (JAN 5 1996) O Kanie, F Barresi, YL Ding, J Labbe, A Otter, LS Forsberg, B Ernst, O Hindsgaul A strategy of ''random glycosylation'' for the production of oligosaccharide libraries 2720-2722 Tetrahedron Letters 37: 1 (JAN 1 1996) S Sasaki, M Takagi, Y Tanaka, M Maeda A new application of a peptide library to identify selective interaction between small peptides in an attempt to develop recognition molecules toward protein surfaces 85-88 Tetrahedron Letters 37: 1 (JAN 1 1996) M Cardno, M Bradley A simple multiple release system for combinatorial library and peptide analysis 135-138 Trends in Biochemical Sciences 21: 1 (JAN 1996) K Udaka Decrypting class I MHC-bound peptides with peptide libraries 7-11 Journal of Medicinal Chemistry 39: 2 (JAN 19 1996) PL Zhao, RB Nachbar, JA Bolognese, K Chapman Two new criteria for choosing sample size in combinatorial chemistry 350-352 Gene 167: 1-2 (DEC 29 1995) A Cabibbo, E Sporeno, C Toniatti, S Altamura, R Savino, G Paonessa, G Ciliberto Monovalent phage display of human interleukin (hIL)-6: Selection of superbinder variants from a complex molecular repertoire in the hIL-6 D-helix 41-47 Gene 167: 1-2 (DEC 29 1995) AJ Vanzonneveld, BMM Vandenberg, M Vanmeijer, H Pannekoek Identification of functional interaction sites on proteins using bacteriophage-displayed random epitope libraries 49-52 Journal of Molecular Biology 255: 5 (FEB 9 1996) TGM Schmidt, J Koepke, R Frank, A Skerra Molecular interaction between the Strep-tag affinity peptide and its cognate target, streptavidin 753-766 Biochemical and Biophysical Research Communications 218: 1 (JAN 5 1996) NT Lin, FS Wen, YH Tseng A region of the filamentous phage phi Lf genome that can support autonomous replication and miniphage production 12-16 Biochemical and Biophysical Research Communications 218: 1 (JAN 5 1996) J Gui, T Moyana, J Xiang Selection of a peptide with affinity for the tumor- associated TAG72 antigen from a phage-displayed library 414-419 Biochemistry 35: 1 (JAN 9 1996) G Liu, RT Bryant, RH Hilderman Isolation of a tripeptide from a random phage peptide library that inhibits P-1,P-4-diadenosine 5'- tetraphosphate binding to its receptor 197-201 European Journal of Biochemistry 234: 3 (DEC 15 1995) K Morimatsu, T Horii DNA-binding surface of RecA protein - Photochemical cross-linking of the first DNA binding site on RecA filament 695-705 Virology 215: 1 (JAN 1 1996) PP Sanna, A Delogu, RA Williamson, YL Hom, SE Straus, FE Bloom, DR Burton Protection of nude mice by passive immunization with a type-common human recombinant monoclonal antibody against HSV 101-106 Journal of Molecular Biology 255: 1 (JAN 12 1996) R Schier, J Bye, G Apell, A Mccall, GP Adams, M Malmqvist, LM Weiner, JD Marks Isolation of high-affinity monomeric human Anti-c- erbB-2 single chain Fv using affinity-driven selection 28-43 Journal of Molecular Biology 255: 1 (JAN 12 1996) F Martin, C Toniatti, AL Salvati, G Ciliberto, R Cortese, M Sollazzo Coupling protein design and in vitro selection strategies: Improving specificity and affinity of a designed beta-protein IL-6 antagonist 86-97 Journal of Molecular Biology 255: 3 (JAN 26 1996) CM Gates, WPC Stemmer, R Kaptein, PJ Schatz Affinity selective isolation of ligands from peptide libraries through display on a lac repressor ''headpiece dimer'' 373-386 From owner-repertoires@net.bio.net Mon Mar 11 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: "Janet Clench, Library, Tel:(39 6)91093220" Newsgroups: bionet.molecules.repertoires Subject: And the next ... Date: 11 Mar 1996 13:42:28 -0000 Lines: 142 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4i1ak4$aai@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk ******************************************************** SUBJECT: Combinatorial & Phage Libraries DATE: February 26, March 6 1996 ******************************************************** Journal of Biological Chemistry 270: 48 (DEC 1 1995) MK Short, PD Jeffrey, RF Kwong, MN Margolies Contribution of antibody heavy chain CDR1 to digoxin binding analyzed by random mutagenesis of phage- displayed Fab 26-10 28541-28550 Peptide Research 8: 5 (SEP-OCT 1995) C Pinilla, S Chendra, JR Appel, RA Houghten Elucidation of monoclonal antibody polyspecificity using a synthetic combinatorial library 250-257 Journal of the American Chemical Society 117: 47 (NOV 29 1995) R Malin, R Steinbrecher, J Jannsen, W Semmler, B Noll, B Johannsen, C Frommel, W Hohne, J Schneidermergener Identification of technetium-99m binding peptides using combinatorial cellulose-bound peptide libraries 11821-11822 Journal of Immunological Methods 187: 1 (NOV 16 1995) HS Dekoster, R Amons, WE Benckhuijsen, M Feijlbrief, GA Schellekens, JW Drijfhout The use of dedicated peptide libraries permits the discovery of high affinity binding peptides 179-188 Gene 165: 2 (NOV 20 1995) CW Bell, KBG Scholthof, GS Zhang, AE Karu Sequences of the cDNAs encoding the heavy- and light-chain Fab region of an antibody to the phenylurea herbicide diuron 323-324 Journal of Molecular Biology 254: 3 (DEC 1 1995) WP Yang, K Green, S Pinzsweeney, AT Briones, DR Burton, CF Barbas CDR walking mutagenesis for the affinity maturation of a potent human Anti-HIV-1 antibody into the picomolar range 392-403 Angewandte Chemie - International Edition in English 35: 2 (FEB 2 1996) K Burgess, HJ Lim, AM Porte, GA Sulikowski New catalysts and conditions for a C-H insertion reaction identified by high throughput catalyst screening 220-222 Angewandte Chemie - International Edition in English 35: 1 (JAN 19 1996) JS Fruchtel, G Jung Organic chemistry on solid supports 17-42 Chemical Reviews 96: 1 (JAN-FEB 1996) LA Thompson, JA Ellman Synthesis and applications of small molecule libraries 555-600 Journal of the American Chemical Society 118: 3 (JAN 24 1996) MM Murphy, JR Schullek, EM Gordon, MA Gallop Combinatorial organic synthesis of highly functionalized pyrrolidines: Identification of a potent angiotensin converting enzyme inhibitor from a mercaptoacyl proline Library, (vol 117, pg 7029, 1995) 714 Tetrahedron Letters 37: 5 (JAN 29 1996) AF Spatola, K Darlak, P Romanovskis An approach to cyclic peptide libraries: Reducing epimerization in medium sized rings during solid phase synthesis 591-594 Journal of Biological Chemistry 271: 5 (FEB 2 1996) LY Chen, DY Chen, J Miaw, NT Hu XpsD, an outer membrane protein required for protein secretion by Xanthomonas campestris pv campestris, forms a multimer 2703-2708 Trends in Pharmacological Sciences 17: 1 (JAN 1996) JA Boutin, AL Fauchere Combinatorial peptide synthesis: Statistical evaluation of peptide distribution 8-12 Biotechnology Annual Review, Vol 1 (Series: Biotechnology Annual Review 1 (1995)) F Felici, A Luzzago, P Monaci, A Nicosia, M Sollazzo, C Traboni Peptide and protein display on the surface of filamentous bacteriophage 149-183 Biochemical and Biophysical Research Communications 218: 3 (JAN 26 1996) K Maenaka, M Furuta, K Tsumoto, K Watanabe, Y Ueda, I Kumagai A stable phage-display system using a phagemid vector: Phage display of hen egg-white lysozyme (HEL), Escherichia coli alkaline, phosphatase, and anti- HEL monoclonal antibody, HyHEL10 682-687 Biochemistry 35: 4 (JAN 30 1996) M Soekarjo, M Eisenhawer, A Kuhn, H Vogel Thermodynamics of the membrane insertion process of the M13 procoat protein, a lipid bilayer traversing protein containing a leader sequence 1232-1241 Journal of Immunological Methods 189: 1 (JAN 16 1996) RL Ward, MA Clark, J Lees, NJ Hawkins Retrieval of human antibodies from phage-display libraries using enzymatic cleavage 73-82 Journal of Molecular Biology 256: 1 (FEB 16 1996) J Thompson, T Pope, JS Tung, C Chan, G Hollis, G Mark, KS Johnson Affinity maturation of a high-affinity human monoclonal antibody against the third hypervariable loop of human immunodeficiency virus: Use of phage display to improve affinity and broaden strain reactivity 77-88 From owner-repertoires@net.bio.net Mon Mar 11 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: Franz Weilbach Newsgroups: bionet.molecules.repertoires Subject: phage display for human ab Date: 11 Mar 1996 08:52:49 -0000 Lines: 11 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4i0pl1$9hk@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk I am new in this field and just entered this newsgroup. Question: Is there a commercial phage display system available for human antibodies. Can anybody recommend a special kit? Thanks in advance Franz Weilbach MD neuk138@rzbox.uni-wuerzburg.de Josef-Schneider-Str. 11 D-97080 Wuerzburg Fax: 49-931-201-3489 From owner-repertoires@net.bio.net Mon Mar 11 22:00:00 1996 Path: biosci!rutgers!csn!news-1.csn.net!carbon!night.primate.wisc.edu!sdd.hp.com!swrinde!newsfeed.internetmci.com!news.internetMCI.com!darwin.sura.net!maze.dpo.uab.edu!copley.microbio.uab.edu!user From: peter_prevelige@micro.microbio.uab.edu (Peter E. Prevelige Jr.) Newsgroups: bionet.molecules.repertoires Subject: Postive Controls? Date: Tue, 12 Mar 1996 09:39:18 -0500 Organization: Dept. of Microbiology, Univ. of Alabama Birmingham Lines: 11 Message-ID: NNTP-Posting-Host: copley.microbio.uab.edu Hello Everyone, We are trying to isolate peptides that interfere with subunit association and are having a number of technical difficulties. In order to debug the system it would be very useful if we had a positive control. If someone has some suggestions, or a specific clone and ligand that they can spare we would appreciate it very much. Thanks Peter Prevelige From owner-repertoires@net.bio.net Thu Mar 14 22:00:00 1996 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!munnari.OZ.AU!news.mel.connect.com.au!harbinger.cc.monash.edu.au!lugb.latrobe.edu.au!newsmgr From: Mick Foley Newsgroups: bionet.molecules.repertoires Subject: Re: phage display for human ab Date: 15 Mar 1996 00:16:06 GMT Organization: LaTrobe University Lines: 22 Message-ID: <4iacs6$nfs@lugb.latrobe.edu.au> References: <4i0pl1$9hk@mserv1.dl.ac.uk> NNTP-Posting-Host: bcmf1.biochem.latrobe.edu.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) There are two kits for phage display of which I am aware, one is the Pharmacia RPAS and the other is the SurfZAP system from Stratagene. Both can be used with human abs if you synthesise (or borrow) the human primers with compatible restriction sites. We have used the Pharmacia kit for mouse Abs (not human yet) with surprising sucess. We combined this kit with a number of other techniques (purification of RNA, amplification of VH and VL etc) that we already were using in the lab. Although we had a bit of trouble we now find the pCANTAB vectors behave very well and we get nice soluble scFvs. I have not used the Stratagene kit (has anybody???). However my experience with the Paharmacia kit has been that the kit is very useful and has helped us get to where we are quickly, but do not have blind faith in it (and I guess this can be applied to any kit, even bicycle repair kits!). Sorry if I have wandered off the track a bit, but I thought a few philosphical points from down under might be useful to someone somewhere. Cheers, Mick From owner-repertoires@net.bio.net Wed Mar 27 22:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molecules.repertoires Subject: IMPORTANT - BIOSCI Fundraising Update! Date: 28 Mar 1996 02:00:36 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 149 Sender: daemon@net.bio.net Distribution: world Message-ID: <199603281000.CAA15850@net.bio.net> NNTP-Posting-Host: net.bio.net I'm interrupting the usual monthly posting of the BIOSCI miniFAQ to bring you up to date on BIOSCI fundraising progress, a topic of concern to your future use of this resource. Thank you in advance for taking the time to read this message carefully. Last year we announced that BIOSCI was going to adopt the U.S. Public Broadcasting System model to fund its operations after our DOE/NSF grant runs out later this year. Unlike PBS, we are not soliciting contributions from users; we are only selling ads on our Web pages solely to cover our operating costs. Our goal is to seek sponsorships until we build up an operating reserve of about $100,000 and then cease further promotions until we need to build the reserve back up. (The accountants among our readership will be familiar with the problem of deferred revenue which we can not safely utilize until ads have been displayed for a period of time.) We have three sponsors to date with a couple more pending. The process is time-consuming, however, and we need your help as explained further below. Our operating costs consist of our network connection, phone lines, hardware maintenance (we hope to have new and faster hardware soon!), plus 0.7 FTE of salaries covering UNIX systems admin, technical support, quality assurance, i.e., testing, of our system, and administrative costs (such as the time it takes to actually find/write/call potential sponsors and raise money!). Although the BIOSCI staff does get compensated for a portion of the work that they do, this project has always received a lot of free after-hours and "vacation" time labor, so we hope that no one will begrudge the time that we do charge to the project to serve you. All of the three part-time staff members, Dave Mack, Julie Lawrence, and myself, have full time day jobs and families in addition to working hard to keep this service running for all of you. Julie and Dave Mack are subcontractors for BIOSCI; my time that is charged to the project defrays a portion of my regular salary instead of adding to my income. Besides having to relocate the project, we were very busy this last year building new infrastructure such as our WWW hypermail interface to the system. This was released last December along with scores of WAIS indices for the newsgroups. Virtually everything is complete, although we do continue to find and fix bugs (many through your helpful feedback!). We are still having some problems with our WAIS indexing. The archives continue to grow rapidly. We are running over 100 indexes now versus three previously and any systems crashes cause greater havoc with the indexing than before! We are still working to fix this as fast as our resources permit and appreciate your patience, but we have been able to automate a lot of the infrastructure to reduce labor as compared to past requirements. We have also implemented new software to make moderation of BIOSCI/bionet newsgroups much easier and combat the growing problem of Internet junk mail and USENET "spamming." About 20% of our groups are now moderated, many of them by the BIOSCI staff! This, for example, made a major difference last year in the quality of content in our EMPLOYMENT/bionet.jobs.offered newsgroup which many commercial concerns and recruiting firms are using **without charge** to recruit candidates for positions in the biological sciences. We are also now in a position to have sponsors for individual newsgroups as you will have noticed if you have visited http://www.bio.net/ and clicked on "Access the BIOSCI/bionet newsgroups" recently. So, how can you help?? ---------------------- As noted above it can take a lot of time to contact potential sponsors if I have to do it all myself. Our request is quite simple. You can do two important things which will take very little time for you individually. First, please use our WWW system at http://www.bio.net/ to access the archives. You can now post or reply to messages via your Web browser. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. Our hope is to quickly raise several large corporate/institutional sponsors on our heavily-used WWW locations (some stats appended below), and then end this sponsorship campaign so that our resources can continue to be used for service provision, not fundraising. Many of our specialty newsgroup WWW archives are still used by small communities of scientists (and they haven't been heavily promoted yet). While these may be valuable niche markets to some advertisers, it will generate more labor and overhead having to find these sponsors, fairly price the locations, and deal with lots of smaller sponsorships than fewer mid-to large sponsors. We are striving to keep our operation as lean and efficient as possible since we are not trying to make careers out of running BIOSCI. We are trying if at all possible to avoid the administrative overhead entailed with processing lots of small payments to reach our fundraising goals. I'd like to thank all of you for your help in advance. In helping us, you are also helping yourselves, not only in keeping this resource available for all of the both large and small research communities that we serve, but also by alleviating the need for us to go back and compete with researchers for tight grant dollars! We promised NSF when we were awarded the BIOSCI grant that we would carry out this mission to make the service self-supporting. With your help, we will succeed in continuing BIOSCI's work into its second decade. Thank you very much! Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net A list of our prime WWW sponsorship locations follow. Statistics are for the four week period from 22 Jan. - 18 Feb. 1996 and usage continues to grow. ---------------------------------------------------------------------- The overall BIOSCI WWW pages are currently visited by users from close to 5000 unique computer hosts per week. Web servers only log the Internet computer/host name and frequently more than one individual can connect to us from a particular host. Main home page, http://www.bio.net, visited recently by about 2100 unique hosts per week Main Newsgroups archives page, http://www.bio.net/archives.html, visited recently by about 1200 Unique hosts per week BIO-JOURNALS archive page, http://www.bio.net/BIO-JOURNALS.html, visited recently by about 1000 unique hosts per week. EMPLOYMENT archive pages: http://www.bio.net:80/hypermail/EMPLOYMENT/ and monthly header pages, visited recently by about 600 unique hosts per week. Address database search page, http://www.bio.net/addrsearch.html, visited recently by about 450 unique hosts per week. Methods newsgroup archive pages, http://www.bio.net:80/hypermail/METHDS- REAGNTS/ and monthly header pages, visited recently by about 350 unique hosts per week. ---------------------------------------------------------------------- From owner-repertoires@net.bio.net Thu Mar 28 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: "Janet Clench, Library, Tel:(39 6)91093220" Newsgroups: bionet.molecules.repertoires Subject: and after this long pause, here's a somewhat out of date update ... Date: 29 Mar 1996 17:22:54 -0000 Lines: 142 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4jh69e$6l3@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk ******************************************************** SUBJECT: Combinatorial & Phage Libraries DATE: February 26, March 6 1996 ******************************************************** Journal of Biological Chemistry 270: 48 (DEC 1 1995) MK Short, PD Jeffrey, RF Kwong, MN Margolies Contribution of antibody heavy chain CDR1 to digoxin binding analyzed by random mutagenesis of phage- displayed Fab 26-10 28541-28550 Peptide Research 8: 5 (SEP-OCT 1995) C Pinilla, S Chendra, JR Appel, RA Houghten Elucidation of monoclonal antibody polyspecificity using a synthetic combinatorial library 250-257 Journal of the American Chemical Society 117: 47 (NOV 29 1995) R Malin, R Steinbrecher, J Jannsen, W Semmler, B Noll, B Johannsen, C Frommel, W Hohne, J Schneidermergener Identification of technetium-99m binding peptides using combinatorial cellulose-bound peptide libraries 11821-11822 Journal of Immunological Methods 187: 1 (NOV 16 1995) HS Dekoster, R Amons, WE Benckhuijsen, M Feijlbrief, GA Schellekens, JW Drijfhout The use of dedicated peptide libraries permits the discovery of high affinity binding peptides 179-188 Gene 165: 2 (NOV 20 1995) CW Bell, KBG Scholthof, GS Zhang, AE Karu Sequences of the cDNAs encoding the heavy- and light-chain Fab region of an antibody to the phenylurea herbicide diuron 323-324 Journal of Molecular Biology 254: 3 (DEC 1 1995) WP Yang, K Green, S Pinzsweeney, AT Briones, DR Burton, CF Barbas CDR walking mutagenesis for the affinity maturation of a potent human Anti-HIV-1 antibody into the picomolar range 392-403 Angewandte Chemie - International Edition in English 35: 2 (FEB 2 1996) K Burgess, HJ Lim, AM Porte, GA Sulikowski New catalysts and conditions for a C-H insertion reaction identified by high throughput catalyst screening 220-222 Angewandte Chemie - International Edition in English 35: 1 (JAN 19 1996) JS Fruchtel, G Jung Organic chemistry on solid supports 17-42 Chemical Reviews 96: 1 (JAN-FEB 1996) LA Thompson, JA Ellman Synthesis and applications of small molecule libraries 555-600 Journal of the American Chemical Society 118: 3 (JAN 24 1996) MM Murphy, JR Schullek, EM Gordon, MA Gallop Combinatorial organic synthesis of highly functionalized pyrrolidines: Identification of a potent angiotensin converting enzyme inhibitor from a mercaptoacyl proline Library, (vol 117, pg 7029, 1995) 714 Tetrahedron Letters 37: 5 (JAN 29 1996) AF Spatola, K Darlak, P Romanovskis An approach to cyclic peptide libraries: Reducing epimerization in medium sized rings during solid phase synthesis 591-594 Journal of Biological Chemistry 271: 5 (FEB 2 1996) LY Chen, DY Chen, J Miaw, NT Hu XpsD, an outer membrane protein required for protein secretion by Xanthomonas campestris pv campestris, forms a multimer 2703-2708 Trends in Pharmacological Sciences 17: 1 (JAN 1996) JA Boutin, AL Fauchere Combinatorial peptide synthesis: Statistical evaluation of peptide distribution 8-12 Biotechnology Annual Review, Vol 1 (Series: Biotechnology Annual Review 1 (1995)) F Felici, A Luzzago, P Monaci, A Nicosia, M Sollazzo, C Traboni Peptide and protein display on the surface of filamentous bacteriophage 149-183 Biochemical and Biophysical Research Communications 218: 3 (JAN 26 1996) K Maenaka, M Furuta, K Tsumoto, K Watanabe, Y Ueda, I Kumagai A stable phage-display system using a phagemid vector: Phage display of hen egg-white lysozyme (HEL), Escherichia coli alkaline, phosphatase, and anti- HEL monoclonal antibody, HyHEL10 682-687 Biochemistry 35: 4 (JAN 30 1996) M Soekarjo, M Eisenhawer, A Kuhn, H Vogel Thermodynamics of the membrane insertion process of the M13 procoat protein, a lipid bilayer traversing protein containing a leader sequence 1232-1241 Journal of Immunological Methods 189: 1 (JAN 16 1996) RL Ward, MA Clark, J Lees, NJ Hawkins Retrieval of human antibodies from phage-display libraries using enzymatic cleavage 73-82 Journal of Molecular Biology 256: 1 (FEB 16 1996) J Thompson, T Pope, JS Tung, C Chan, G Hollis, G Mark, KS Johnson Affinity maturation of a high-affinity human monoclonal antibody against the third hypervariable loop of human immunodeficiency virus: Use of phage display to improve affinity and broaden strain reactivity 77-88 From owner-repertoires@net.bio.net Thu Mar 28 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Phage display of antibodies Date: 29 Mar 1996 10:30:16 -0000 Lines: 35 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4jge3o$2ob@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk, neuk138@rzbox.uni-wuerzburg.de In bionet.molecules.repertoires Msg # 110 you wrote: >I am new in this field and just entered this newsgroup. >Question: Is there a commercial phage display system available for human >antibodies. Can anybody recommend a special kit? > >Thanks in advance > >Franz Weilbach MD >neuk138@rzbox.uni-wuerzburg.de I have heard that there is a company called Cambridge Antibody Technology based in England which sells a Fab-phage display system but I don't know much about it. However if you are at a University you may be able to get a library from Greg Winter's group at the MRC-LMB in Cambridge, England. You will have to sign an agreement in order to be supplied with the library but there should be no problem about this if you are with a university or other non-commercial organisation. Cheers, Andrew Andrew Wallace School of Biology and Biochemistry The Queen's University of Belfast 97 Lisburn Road, Belfast BT9 7BL, N. Ireland (UK) a.wallace@queens-belfast.ac.uk