From owner-repertoires@net.bio.net Sat May 04 23:00:00 1996 Path: biosci!ARGOTECH.COM!pvaneikeren From: pvaneikeren@ARGOTECH.COM (Paul van Eikeren) Newsgroups: bionet.molecules.repertoires Subject: 1996 GRC Biocatalysis Date: 5 May 1996 16:52:11 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 133 Sender: daemon@net.bio.net Distribution: world Message-ID: <01BB3AA2.B411D0C0@bend42.bendnet.com> NNTP-Posting-Host: net.bio.net =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D Gordon Research Conference B I O C A T A L Y S I S Kimbal Union Academy * Meriden, NH, USA * 7-12 July 1996 =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D http://w3.argotech.com/argotech/biocatalysis I am posting this message to inform you of this years' Gordon Research = Conference on Biocatalysis. The Conference will be held 7-12 July 1996 = at Kimbal Union Academy in Meriden, New Hampshire, USA. The Conference = brings together an interdisciplinary group of biologists, chemists, and = engineers for a full week of intense discussion and examination of the = frontiers of Biocatalysis. We welcome your application for = participation in year's conference. Also, we would be grateful if you = would forward a copy of this message to people that you think might be = interested in attending. The subject of the Biocatalysis Conference is synthetically useful = reactions and processes catalyzed by enzymes or whole cells. We will = address three main themes: * new uses of existing enzymes (e.g., lipases, oxidases, and aldolases); * discovery of new enzymes (e.g., epoxide hydrolases, P-450 = hydroxylases, oxynitrilases, thermophilic organisms); and * structure and protein engineering of enzymes (e.g., new structures of = proteases, transketolase and oxynitrilase, and combinatorial methods = versus site-directed mutagenesis).=20 We have assembled a notable group of speakers, discussion leaders, and = poster presenters. Attached is a copy of the preliminary program. For = additional information on the Conference including the most recent = update of the program and the poster sessions we suggest that you = connect to our World-Wide Web site at http://w3.argotech.com/argotech/biocatalysis If you do not have access to the World-Wide Web or need additional = information, please contact me by e-mail at the address shown below and = can send you additional information and applications forms. We look forward to receiving your application to the conference. Sincerely, Paul van Eikeren =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D Paul van Eikeren, Co-Chair Vice President, Chemistry Argonaut Technologies Inc. 887 Industrial Road, Suite G San Carlos, CA 94070 USA Voice: +1 415 598 1350 ext. 217 Fax: +1 415 598 1359 pvaneikeren@argotech.com 74260.1024@compuserve.com =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D P R O G R A M I N F O R M A T I O N (Updated on 25 March 1996) =3D=3D Opening Session: * Stanley Roberts (Liverpool, UK) Enzymic Baeyer Villiger Reaction * J. John Holbrook (U. Bristol, UK) Getting the Products Off Enzymes = Used in Bulk Chemoenzymic Synthesis =3D=3D Structure and Engineering of Hydrolases for Organic Synthesis * Sabine L. Flitsch (U. Edinburgh, UK) Design and Synthesis of = Enzyme-Cleavable Linkers for Solid Phase Synthesis * Guy G. Dodson (York U., UK) Nucleophilic Attack at the Carbonyl in = Hydrolases: The Varying Stereochemistry at the Nucleophile * Franz Effenberger (U. Stuttgart, Germany) New Results on Preparation = and Application of Chiral Cyanohydrins * Robert Menard (NRC Biotechnology Research Institute, Canada) Protein = Engineering of Cysteine Proteases: From a Better Understanding of Their = Function to Redesigning the Catalytic Activity =3D=3D Molecular Evolution of Subtilisin * Frances Arnold (California Institute of Technology, USA) Directed = Evolution of p-Nitrobenzyl Esterase * Marcus Ballinger (Genentech, USA) Subtilisin BPN Variants Designed for = Cleavage of Multibasic Substrates Multibasic Substrates=20 * Volker Schellenberger (Genencor, USA) Directed evolution of a = Bacillus protease =3D=3D New Application of Hydrolases * Herbert Waldmann (Karlsruhe, Germany) Bioorganic Synthesis and = Biological Signal Transduction * Milton Zmijewski (Lily, Indianapolis, USA) Enzymatic Removal of = Protecting Groups in the Synthesis of Pharmaceuticals * Kazuo Achiwa (Shizuoka U.) Lipase-Catalyzes Asymmetric Synthesis of = Optically-Active Medicines: New Strategies and their Application =3D=3D Discovery versus Engineering of New Enzymes * John Arnett (Recombinant Biocatalysis, USA) Enzymes from Thermophilic = Microorganisms * Gunter Schneider (Karolinska Institute, Stockholm, Sweden) Toward = Tailoring Enzymes for Asymmetric Synthesis: Protein Engineering of = Transketolase =3D=3D Aldolases and Glycosyl Transfer * Eric Toone (Duke University) Pyruvate Aldolases * Vladimir Kren (Czech Academy of Sciences) Enzymatic Glycosylation of = Pharmacologically Active Compounds: Multienzymatic Approaches and New = Enzymes =3D=3D New Hydrolases * Roland Furstoss (U. Aix-Marseille, France) Enantioselective = Biotransformations with Epoxide Hydrolases * Kenji Soda (Kyoto, Japan) 2-Haloacid Dehalogenases: Their Functions, = Structures, and Applications =3D=3D Oxidations * Aleksey Zaks (Schering-Plough, Union, NJ, USA) Chloroperoxidase * Bernhard Hauer (BASF AG, Ludwigshafen, Germany) Selection of = Biocatalysts for the Preparation of Chiral/Chemical Building Blocks * Roger Sheldon (Delft U. Netherlands) Enantioselective Aminolysis and = Selective Oxidations Mediated by Chloroperoxidase =3D=3D Large Scale Industrial Applications * Kevin DiGregorio (Union Carbide, USA) Polyester Hydrolysis * Robert Dicosimo (Dupont, Wilmington, DE, USA) Scale-Up of a = Biocatalytic Route to N-Phosphonomethylglycine (Glyphosate) Using Whole = Cell Transformants From owner-repertoires@net.bio.net Sun May 05 23:00:00 1996 Path: biosci!ARGOTECH.COM!pvaneikeren From: pvaneikeren@ARGOTECH.COM (Paul van Eikeren) Newsgroups: bionet.molecules.repertoires Subject: 1996 GRC Biocatalysis Date: 5 May 1996 17:10:51 -0700 Organization: Argonaut Technologies Inc. Lines: 174 Sender: daemon@net.bio.net Distribution: world Message-ID: <318D42CF.48AA@argotech.com> NNTP-Posting-Host: net.bio.net =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D Gordon Research Conference B I O C A T A L Y S I S Kimbal Union Academy * Meriden, NH, USA * 7-12 July 1996 =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D http://w3.argotech.com/argotech/biocatalysis I am posting this message to inform you of this years=92 Gordon Research = Conference on Biocatalysis. The Conference will be held 7-12 July 1996 = at Kimbal Union Academy in Meriden, New Hampshire, USA. The Conference = brings together an interdisciplinary group of biologists, chemists, and = engineers for a full week of intense discussion and examination of the = frontiers of Biocatalysis. We welcome your application for = participation in year=92s conference. Also, we would be grateful if you = would forward a copy of this message to people that you think might be = interested in attending. The subject of the Biocatalysis Conference is synthetically useful = reactions and processes catalyzed by enzymes or whole cells. We will = address three main themes: =B7 new uses of existing enzymes (e.g., lipases, oxidases, and aldolases); =B7 discovery of new enzymes (e.g., epoxide hydrolases, P-450 = hydroxylases, oxynitrilases, thermophilic organisms); and =B7 structure and protein engineering of enzymes (e.g., new structures of = proteases, transketolase and oxynitrilase, and combinatorial methods = versus site-directed mutagenesis). = We have assembled a notable group of speakers, discussion leaders, and = poster presenters. Attached is a copy of the preliminary program. For = additional information on the Conference including the most recent = update of the program and the poster sessions we suggest that you = connect to our World-Wide Web site at http://w3.argotech.com/argotech/biocatalysis If you do not have access to the World-Wide Web or need additional = information, please contact me by e-mail at the address shown below and = can send you additional information and applications forms. We look forward to receiving your application to the conference. Sincerely, Paul van Eikeren =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D Paul van Eikeren, Co-Chair Vice President, Chemistry Argonaut Technologies Inc. 887 Industrial Road, Suite G San Carlos, CA 94070 USA Voice: +1 415 598 1350 ext. 217 Fax: +1 415 598 1359 pvaneikeren@argotech.com 74260.1024@compuserve.com =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D P R O G R A M I N F O R M A T I O N (Updated on 25 March 1996) =3D=3D Opening Session: * Stanley Roberts (Liverpool, UK) Enzymic Baeyer Villiger Reaction * J. John Holbrook (U. Bristol, UK) Getting the Products Off Enzymes = Used in Bulk Chemoenzymic Synthesis =3D=3D Structure and Engineering of Hydrolases for Organic Synthesis * Sabine L. Flitsch (U. Edinburgh, UK) Design and Synthesis of = Enzyme-Cleavable Linkers for Solid Phase Synthesis * Guy G. Dodson (York U., UK) Nucleophilic Attack at the Carbonyl in = Hydrolases: The Varying Stereochemistry at the Nucleophile * Franz Effenberger (U. Stuttgart, Germany) New Results on Preparation = and Application of Chiral Cyanohydrins * Robert Menard (NRC Biotechnology Research Institute, Canada) Protein = Engineering of Cysteine Proteases: From a Better Understanding of Their = Function to Redesigning the Catalytic Activity =3D=3D Molecular Evolution of Subtilisin * Frances Arnold (California Institute of Technology, USA) Directed = Evolution of p-Nitrobenzyl Esterase * Marcus Ballinger (Genentech, USA) Subtilisin BPN Variants Designed for = Cleavage of Multibasic Substrates Multibasic Substrates = * Volker Schellenberger (Genencor, USA) Directed evolution of a = Bacillus protease =3D=3D New Application of Hydrolases * Herbert Waldmann (Karlsruhe, Germany) Bioorganic Synthesis and = Biological Signal Transduction * Milton Zmijewski (Lily, Indianapolis, USA) Enzymatic Removal of = Protecting Groups in the Synthesis of Pharmaceuticals * Kazuo Achiwa (Shizuoka U.) Lipase-Catalyzes Asymmetric Synthesis of = Optically-Active Medicines: New Strategies and their Application =3D=3D Discovery versus Engineering of New Enzymes * John Arnett (Recombinant Biocatalysis, USA) Enzymes from Thermophilic = Microorganisms * Gunter Schneider (Karolinska Institute, Stockholm, Sweden) Toward = Tailoring Enzymes for Asymmetric Synthesis: Protein Engineering of = Transketolase =3D=3D Aldolases and Glycosyl Transfer * Eric Toone (Duke University) Pyruvate Aldolases * Vladimir Kren (Czech Academy of Sciences) Enzymatic Glycosylation of = Pharmacologically Active Compounds: Multienzymatic Approaches and New = Enzymes =3D=3D New Hydrolases * Roland Furstoss (U. Aix-Marseille, France) Enantioselective = Biotransformations with Epoxide Hydrolases * Kenji Soda (Kyoto, Japan) 2-Haloacid Dehalogenases: Their Functions, = Structures, and Applications =3D=3D Oxidations * Aleksey Zaks (Schering-Plough, Union, NJ, USA) Chloroperoxidase * Bernhard Hauer (BASF AG, Ludwigshafen, Germany) Selection of = Biocatalysts for the Preparation of Chiral/Chemical Building Blocks * Roger Sheldon (Delft U. Netherlands) Enantioselective Aminolysis and = Selective Oxidations Mediated by Chloroperoxidase =3D=3D Large Scale Industrial Applications * Kevin DiGregorio (Union Carbide, USA) Polyester Hydrolysis * Robert Dicosimo (Dupont, Wilmington, DE, USA) Scale-Up of a = Biocatalytic Route to N-Phosphonomethylglycine (Glyphosate) Using Whole = Cell Transformants From owner-repertoires@net.bio.net Mon May 06 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: "Janet Clench, Library, Tel:(39 6)91093220" Newsgroups: bionet.molecules.repertoires Subject: After another long pause, a spring collection of refs for you ... Date: 7 May 1996 11:54:28 +0100 Lines: 264 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4mna54$g7j@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk ******************************************************** SUBJECT: Combinatorial & Phage Libraries DATE: 8,15,22,29 April 1996 ******************************************************* Journal of Biological Chemistry 271: 14 (APR 5 1996) SH Baek, JK Seo, CB Chae, PG Suh, SH Ryu Identification of the peptides that stimulate the phosphoinositide hydrolysis in lymphocyte cell lines from peptide libraries 8170-8175 Peptide Research 9: 1 (JAN-FEB 1996) R Toomik, M Edlund, P Ek, B Obrink, L Engstrom Simultaneously synthesized peptides on continuous cellulose membranes as substrates for protein kinases 6-11 Journal of Bacteriology 178: 7 (APR 1996) NA Linderoth, P Model, M Russel Essential role of a sodium dodecyl sulfate-resistant protein IV multimer in assembly-export of filamentous phage 1962-1970 Nature 380: 6574 (APR 11 1996) P Colas, B Cohen, T Jessen, I Grishina, J Mccoy, R Brent Genetic selection of peptide aptamers that recognize and inhibit cyclin-dependent kinase 2 548-550 Journal of Biological Chemistry 271: 13 (MAR 29 1996) M Vanmeijer, Y Roelofs, J Neels, AJG Horrevoets, AJ Vanzonneveld, H Pannekoek Selective screening of a large phage display library of plasminogen activator inhibitor 1 mutants to localize interaction sites with either thrombin or the variable region 1 of tissue-type plasminogen activator 7423-7428 Journal of Biological Chemistry 271: 13 (MAR 29 1996) J Dekruif, T Logtenberg Leucine zipper dimerized bivalent and bispecific scFv antibodies from a semi-synthetic antibody phage display library 7630-7634 Proteins - Structure Function and Genetics 24: 3 (MAR 1996) J Gui, T Moyana, B Malcolm, J Xiang Identification of a decapeptide with the binding reactivity for tumor-associated TAG72 antigen from a phage displayed library 352-358 Bioorganic & Medicinal Chemistry Letters 6: 6 (MAR 19 1996) GC Look, JR Schullek, CP Holmes, JP Chinn, EM Gordon, MA Gallop The identification of cyclooxygenase-1 inhibitors from 4-thiazolidinone combinatorial libraries 707-712 Human Immunology 45: 2 (FEB 1996) S Fujisao, S Matsushita, T Nishi, Y Nishimura Identification of HLA-DR9 (DRB1*0901)-binding peptide motifs using a phage fUSE5 random peptide library 131-136 Gene 169: 2 (MAR 9 1996) R Schier, RF Balint, A Mccall, G Apell, JW Larrick, JD Marks Identification of functional and structural amino-acid residues by parsimonious mutagenesis 147-155 Nature 380: 6572 (MAR 28 1996) R Pasqualini, E Ruoslahti Organ targeting in vivo using phage display peptide libraries 364-366 Science 271: 5257 (MAR 29 1996) TNM Schumacher, LM Mayr, DL Minor, MA Milhollen, MW Burgess, PS Kim Identification of D-peptide ligands through mirror- image phage display 1854-1857 Biochemical and Biophysical Research Communications 220: 1 (MAR 7 1996) R Fang, J Qi, ZB Lu, H Zhou, W Li, JC Shen Selection of trypsin inhibitors in phage peptide library 53-56 Protein Science 5: 1 (JAN 1996) A Tamura, M Matsushita, A Naito, S Kojima, KI Miura, K Akasaka Dynamics of the three methionyl side chains of Streptomyces subtilisin inhibitor. Deuterium NMR studies in solution and in the solid state 127-139 Journal of the American Chemical Society 118: 11 (MAR 20 1996) S Cheng, DD Comer, JP Williams, PL Myers, DL Boger Novel solution phase strategy for the synthesis of chemical libraries containing small organic molecules 2567-2573 Journal of the American Chemical Society 118: 10 (MAR 13 1996) DP Curran, S Hadida Tris(2-(perfluorohexyl)ethyl)tin hydride: A new fluorous reagent for use in traditional organic synthesis and liquid phase combinatorial synthesis 2531-2532 Biotechnology and Bioengineering 50: 2 (APR 20 1996) A Schwienhorst, BF Lindemann, M Eigen Growth kinetics of a bacteriophage in continuous culture 217-221 Molecular & General Genetics 250: 4 (MAR 7 1996) A Degroot, JJ Krijger, A Filloux, J Tommassen Characterization of type II protein secretion (xcp) genes in the plant growth stimulating Pseudomonas putida, strain WCS358 491-504 Accounts of Chemical Research 29: 3 (MAR 1996) SH Dewitt, AW Czarnik Combinatorial organic synthesis using Parke-Davis's DIVERSOMER method 114-122 Accounts of Chemical Research 29: 3 (MAR 1996) RW Armstrong, AP Combs, PA Tempest, SD Brown, TA Keating Multiple-component condensation strategies for combinatorial library synthesis 123-131 Accounts of Chemical Research 29: 3 (MAR 1996) JA Ellman Design, synthesis, and evaluation of small-molecule libraries 132-143 Accounts of Chemical Research 29: 3 (MAR 1996) EM Gordon, MA Gallop, DV Patel Strategy and tactics in combinatorial organic synthesis. Applications to drug discovery 144-154 Accounts of Chemical Research 29: 3 (MAR 1996) WC Still Discovery of sequence-selective peptide binding by synthetic receptors using encoded combinatorial libraries 155-163 Accounts of Chemical Research 29: 3 (MAR 1996) LC Hsiehwilson, XD Xiang, PG Schultz Lessons from the immune system: From catalysis to materials science 164-170 European Journal of Medicinal Chemistry 31: 2 (1996) X Williard, I Pop, L Bourel, D Horvath, R Baudelle, P Melnyk, B Deprez, A Tartar Combinatorial chemistry: A rational approach to chemical diversity 87-98 Molecular Immunology 32: 17-18 (DEC 1995) T Onda, D Laface, G Baier, T Brunner, N Honma, T Mikayama, A Altman, DR Green A phage display system for detection of T cell receptor-antigen interactions 1387-1397 Molecular Immunology 33: 1 (JAN 1996) M Ohlin, H Owman, M Mach, CAK Borrebaeck Light chain shuffling of a high affinity antibody results in a drift in epitope recognition 47-56 Journal of Chemical Information and Computer Sciences 36: 2 (MAR-APR 1996) S Fujita The sphericity concept for an orbit of bonds. Formulation of chirogenic sites in a homospheric orbit and of bond-differentiating chiral reactions with applications to C-60-adducts 270-285 Accounts of Chemical Research 29: 3 (MAR 1996) SH Dewitt, AW Czarnik Combinatorial organic synthesis using Parke-Davis's DIVERSOMER method 114-122 Accounts of Chemical Research 29: 3 (MAR 1996) RW Armstrong, AP Combs, PA Tempest, SD Brown, TA Keating Multiple-component condensation strategies for combinatorial library synthesis 123-131 Accounts of Chemical Research 29: 3 (MAR 1996) JA Ellman Design, synthesis, and evaluation of small-molecule libraries 132-143 Accounts of Chemical Research 29: 3 (MAR 1996) EM Gordon, MA Gallop, DV Patel Strategy and tactics in combinatorial organic synthesis. Applications to drug discovery 144-154 Accounts of Chemical Research 29: 3 (MAR 1996) WC Still Discovery of sequence-selective peptide binding by synthetic receptors using encoded combinatorial libraries 155-163 Accounts of Chemical Research 29: 3 (MAR 1996) LC Hsiehwilson, XD Xiang, PG Schultz Lessons from the immune system: From catalysis to materials science 164-170 Collection of Czechoslovak Chemical Communications 61: 2 (FEB 1996) M Rinnova, M Lebl Molecular diversity and libraries of structures: Synthesis and screening 171-231 Journal of the American Chemical Society 118: 10 (MAR 13 1996) DP Curran, S Hadida Tris(2-(perfluorohexyl)ethyl)tin hydride: A new fluorous reagent for use in traditional organic synthesis and liquid phase combinatorial synthesis 2531-2532 Journal of the American Chemical Society 118: 11 (MAR 20 1996) S Cheng, DD Comer, JP Williams, PL Myers, DL Boger Novel solution phase strategy for the synthesis of chemical libraries containing small organic molecules 2567-2573 From owner-repertoires@net.bio.net Mon May 06 23:00:00 1996 Path: biosci!daresbury!yama.mcc.ac.uk!sunsite.doc.ic.ac.uk!nntp0.brunel.ac.uk!strath-cs!queens-belfast.ac.uk!bcg0197 From: bcg0197@queens-belfast.ac.uk (Andrew Wallace) Newsgroups: bionet.molecules.repertoires,bionet.jobs Subject: Postgraduate studentship available Message-ID: <1996May7.104523.6292@queens-belfast.ac.uk> Date: 7 May 96 10:45:23 GMT Organization: Queen's University of Belfast Lines: 35 A Postgraduate Research Studentship is available in my laboratory commencing in October 1996. Two projects are currently available to choose from: either: use of combinatorial libraries of mechanism-specific active site-directed protease inhibitors to identify novel proteases involved in the regulation of apoptosis. or: generation of catalytic antibodies with proteolytic activity by selection from a phage-displayed antibody library using novel synthetic transition-state analogues. Applications are invited from UK and EU candidates who have or expect to receive a relevant First or Upper Second Class Honours degree or equivalent qualification, or from qualified non-EU international students who can obtain their own funding. For UK and EU canditates, the studentship pays tuition fees, laboratory costs and a living expenses contribution of about 1000 pounds sterling per quarter year. Further details and application forms are available from: The Director, School of Biology and Biochemistry The Queen's University of Belfast 97 Lisburn Road Tel. +44-1232-335786 Belfast BT9 7BL Fax +44-1232-236505 Northern Ireland (UK) sobb.office@queens-belfast.ac.uk All applications must be received by the Admissions Office, The Queen's University of Belfast no later than 15 May 1996 -- ============================================================ Andrew Wallace, Ph.D. School of Biology and Biochemistry From owner-repertoires@net.bio.net Wed May 08 23:00:00 1996 Path: biosci!rutgers!csn!nntp-xfer-1.csn.net!imci3!imci4!newsfeed.internetmci.com!netnews2.nwnet.net!news.u.washington.edu!verlinde From: verlinde@u.washington.edu (Christophe Verlinde) Newsgroups: bionet.molecules.repertoires Subject: POSITION Res. Assistant Professor Date: 9 May 1996 00:55:15 GMT Organization: University of Washington Lines: 29 Message-ID: <4mrfpj$8dl@nntp3.u.washington.edu> NNTP-Posting-Host: saul7.u.washington.edu NNTP-Posting-User: verlinde Keywords: combinatorial chemistry Research Assistant Professor Biomolecular Synthesis and Medicinal Chemistry A Research Faculty position is available in the Department of Biological Structure at the University of Washington for a Research Assistant Professor to carry out synthesis of novel protein inhibitors as well as to employ combinatorial chemistry for the discovery of high affinity protein ligands with special emphasis on infectious diseases and cancer. Interest in structure-based drug design projects is essential. The successful applicant will play a crucial role in the multi-disciplinary Biomolecular Structure Center of the School of Medicine which involves interactions with structural biologists, molecular modelling experts, and protein engineers. The successful applicant will be expected to participate in existing research projects, as well as independently raising funds for collaborative projects. Interested applicants should submit a letter of application, curriculum vitae, statement of career goals and the names and full addresses of three or more referees to: Dr. Wim G.J.Hol, Department of Biological Structure and the Biomolecular Structure Center, University of Washington, Box 357742, Seattle, WA 98195. Review of the applications will begin on May 25, 1996 and continue until the position is filled. The University of Washington is an Equal Opportunity and Affirmative Action Employer. From owner-repertoires@net.bio.net Sun May 12 23:00:00 1996 Path: biosci!daresbury!sunsite.doc.ic.ac.uk!nntp0.brunel.ac.uk!strath-cs!queens-belfast.ac.uk!bcg0197 From: bcg0197@queens-belfast.ac.uk (Andrew Wallace) Newsgroups: bionet.molecules.repertoires Subject: RED ALERT - SwissProt in trouble! Message-ID: <1996May13.142825.6354@queens-belfast.ac.uk> Date: 13 May 96 14:28:25 GMT Organization: Queen's University of Belfast Lines: 12 I heard that the SWISSPROT database is in funding difficulties. Look out for Amos Bairoch's message on bionet.molbio.proteins and set warp factor 5 to his web site to find out what's going on. -- ============================================================ Andrew Wallace, Ph.D. School of Biology and Biochemistry The Queen's University of Belfast 97 Lisburn Road Tel. +44-1232-335786 Belfast BT9 7BL Fax +44-1232-236505 Northern Ireland (UK) a.wallace@queens-belfast.ac.uk From owner-repertoires@net.bio.net Mon May 13 23:00:00 1996 Path: biosci!daresbury!yama.mcc.ac.uk!zippy.dct.ac.uk!str-ccsun!strath-cs!queens-belfast.ac.uk!bcg0197 Newsgroups: bionet.molecules.repertoires Subject: Closure of SWISS-PROT Message-ID: <1996May14.190332.6366@queens-belfast.ac.uk> From: bcg0197@queens-belfast.ac.uk (Andrew Wallace) Date: 14 May 96 19:03:32 GMT Followup-To: bionet.molecules.repertoires,bionet.announce References: <4mv6r7$a8q@mserv1.dl.ac.uk> Distribution: world X-From: X-Newsgroups: bionet.announce X-Relay-Version: ANU News - V6.1B9 05/16/94 VAX/VMS V5.5-2; site queens-belfast.ac.uk X-Date: 10 May 1996 12:24:11 -0700 X-Sender: daemon@net.bio.net X-Organization: BIOSCI International Newsgroups for Molecular Biology X-Approved: biosci-help@net.bio.net X-NNTP-Posting-Host: net.bio.net Lines: 172 SWISS-PROT SHOULD HAVE BEEN 10 YEARS OLD IN JULY 1996, BUT IT MAY DISAPPEAR ON JUNE 30, 1996. Due to funding problems, SWISS-PROT as well as PROSITE, and the ENZYME nomenclature databases will disappear on June 30, 1996 if no solution is found before that date. The ExPASy WWW server and all services associated with it will also shut down. The distribution of the SWISS- 2DPAGE database will also be discontinued. Other external databases, WWW services and software packages that depend on SWISS-PROT, PROSITE and ENZYME as well as on the links provided between biomolecular databases will also be severely affected by this problem. Users of services and databases such as ENTREZ, BLOCKS, SRS, Owl, etc. should also be aware that most annotations at the protein level available through these services originate from SWISS-PROT or PROSITE. While the databases listed above as well as the ExPASy server are used in almost every laboratory doing molecular biology in the world, the funding for these projects has always been very modest (to say the least) and is now, due to procedural problems, going to disappear. [If you are not interested in the details of these problems and you want to send us a email or letter (fax) of support explaining why you think that these resources should stay available to the biological user community, you can skip the following section and jump to the end of this message] SUMMARY OF THE CURRENT SITUATION AND WHAT SHOULD HAVE HAPPENED Currently SWISS-PROT is developed as a collaboration between two sites: - The Medical Biochemistry Department of the University of Geneva, where in addition to the principal investigator of the project (Amos Bairoch), five annotators and a programmer are working on SWISS-PROT and related projects. Three of the five annotators are paid by a Swiss National Fund (FNRS) grant that ends on June 30. One additional annotator position, which is paid for by a special EMBL grant which also ends at the same time. The last position is on a Glaxo academic grant which will end in December. - The EMBL outstation, the European Bioinformatics Institute (EBI) in Hinxton, where six persons are working on SWISS-PROT: a coordinator (Rolf Apweiler), four annotators and a programmer. The EBI has recently embarked on major work to complement SWISS-PROT with a computer annotated supplement called TrEMBL which together with SWISS-PROT produces the first really non-redundant annotated protein sequence database. The completion of this work will require expanded resources at the EBI. About two years ago, a decision was reached in Switzerland that due to the international nature of the SWISS-PROT database, it ought not be funded by money reserved for national projects, but rather from funds intended for projects at the European or International level and to which Switzerland participates. Therefore we were asked to write a grant proposal at the European level. As such a proposal requires participants from at least two or more EU states (which Switzerland is not), an application was submitted in December 1995 which requests: - Five positions in Geneva for the annotators whose contracts will otherwise end in June 1996. - Four positions at EBI, to allow the development of TrEMBL and to cope with the increasing flow of data from genomic projects. - One position in Ireland, with Prof. Keith Tipton, to maintain and update the enzyme nomenclature (EC number) of the International Union of Biology and Molecular Biology (IUBMB). This nomenclature is the backbone of the ENZYME database. - One position at the Weizmann Institute in Israel and a partial position at the company Compugen to develop, in collaboration, the Bioccelerator sequence search hardware engine in ways that will help the maintenance of TrEMBL. - One position at INRIA in France, to develop software in collaboration with Compugen. We have been advised that this proposal was evaluated favorably by the scientific experts of the EU (equivalent of an US Study Section), but was not accepted at a higher level. The main (and apparently only) reason seems to be that those judging the proposal were under the impression that it requested funds solely for new developments. They were unaware that the CURRENT ACTIVITIES could not be maintained without this funding. They also seem to have failed to take note of the fact that the money for the Swiss operation was not coming out of the EU budget, but directly from Swiss government funds, provided that the EU approved the project. They therefore rejected this project while accepting other projects which themselves depend on the existence of SWISS-PROT (for example, a project in which Geneva is also involved, to establish a G-protein linked receptor database which will extend SWISS-PROT to provide information specific to this field of research). Having learned the extent of the problem, the EU seems genuinely concerned but does not seem to have the means of reversing such a decision. They are asking us to resubmit the proposal. But such a process will take almost a year and we only have two months left of salaries.... In Switzerland, money for SWISS-PROT is available, but can not be assigned to such a purpose before the EU accepts the grant. So we are in a catch 22 situation where everyone agrees that there is a problem, that it should be solved, but that they are unable to do anything for procedural reasons ! WHAT CAN WE AND YOU DO ? In the absence of public funding two scenarios seem possible. SWISS-PROT and PROSITE could pass into private hands as proprietary databases, or some non-profit association could be established which would recoup the ENTIRE costs of the operation through subscriptions. Two pharmaceutical companies have already expressed interest in the former solution, and existing examples of the latter are CAS (Chemical abstracts) and CCDC (Cambridge Crystallographic Data Centre). However we see enormous benefits to the user community from the public availability of the data. The first of these solutions would be incompatible with the mission of our partners at the EBI, but if it comes between a complete disappearance and such a solution, there does not seem to be a choice. At the time when there is growing concern about the privatisation of genomic data, we are facing a situation that could lead to the disappearance of what we think are the most widely used information resources on protein sequences because of our reliance on soft public money. We would much prefer to continue to offer and extend services to all the biological user community free of charge. To do so we need your help to convince the various funding agencies that you need these services for your research. We are therefore asking our user community to send emails of support stating why you think that these resources should continue to be available. You can send these messages to: help.sprot@hon.ch Do not forget to include in such an email, your full name, title and organization to which you belong. If you wish to write a letter of support, you can fax it to the following number: + 41-22-346 87 58 Or send it by post to: Amos Bairoch Dept. Medical Biochemistry 1, rue Michel Servet 1211 Geneva 4 Switzerland Many thanks to all of you. Amos Bairoch PS1: Feel free to forward this message to colleagues. PS2: Apologies to all those of you who have (will) receive multiple copies of this message. PS3: This text is also available on WWW at: http://expasy.hcuge.ch/sprot/help-sprot.html -End of message- ------------------- This message was generated on ExPASy. From owner-repertoires@net.bio.net Thu May 16 23:00:00 1996 Path: biosci!daresbury!yama.mcc.ac.uk!bofh.dot!news.salford.ac.uk!aber!bath.ac.uk!dcl-cs!strath-cs!queens-belfast.ac.uk!bcg0197 From: bcg0197@queens-belfast.ac.uk (Andrew Wallace) Newsgroups: bionet.molecules.repertoires Subject: Re: Looking for E-mail address of Dr.M.Lebl Message-ID: <1996May17.121855.6409@queens-belfast.ac.uk> Date: 17 May 96 12:18:55 GMT References: <4nhe59$g77@mserv1.dl.ac.uk> <1996May17.121807.6408@queens-belfast.ac.uk> X-From: bcg0197@queens-belfast.ac.uk (Andrew Wallace) X-Newsgroups: bionet.molecules.peptides Followup-To: bionet.molecules.repertoires,bionet.molecules.peptides Distribution: bionet X-Date: 17 May 96 12:18:07 GMT Distribution: bionet X-Organization: Queen's University of Belfast Lines: 24 In article <4nhe59$g77@mserv1.dl.ac.uk>, writes: > Does anyone know the E-address of Dr. Michal Lebl (formerly from the Institute > of the Organic Chemistry and biochemistry, Prague and then from Selectide Corp., > Tuscon, AZ)? > > > Vladimir Titov > titov@gmdp.siobc.ras.ru > > You can find it if you look at the home page of the journal Molecular Diversity which is on http://vesta.pd.com/ which I suggest you do as he changed it recently and I don't remember his new address. Andrew -- ================================================================ Andrew Wallace, Ph.D. School of Biology and Biochemistry The Queen's University of Belfast Tel. +44-1232-335786 97 Lisburn Road Fax +44-1232-236505 Belfast BT9 7BL a.wallace@queens-belfast.ac.uk Northern Ireland (UK) http://www.qub.ac.uk/b&bchem/rbiochem.htm From owner-repertoires@net.bio.net Tue May 21 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: "Janet Clench, Library, Tel:(39 6)91093220" Newsgroups: bionet.molecules.repertoires Subject: Ans here's the merry month of May .... ciao ciao Date: 22 May 1996 15:35:10 +0100 Lines: 145 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4nv8mu$b5k@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk ********************************************************* SUBJECT: Combinatorial & Phage Libraries DATE: 6, 13, 20 May 1996 ******************************************************** Recombinant DNA Methodology II (Series: Selected Methods in Enzymology Series (1995)) GP Smith, JK Scott Libraries of peptides and proteins displayed on filamentous phage 333-362 Protein Engineering 9: 2 (FEB 1996) A Blondel, R Nageotte, H Bedouelle Destabilizing interactions between the partners of a bifunctional fusion protein 231-238 Bioorganic & Medicinal Chemistry Letters 6: 7 (APR 9 1996) S Vanwetswinkel, J Marchandbrynaert, J Fastrez Selection of the most active enzymes from a mixture of phage-displayed beta-lactamase mutants. 789-792 Journal of the American Chemical Society 118: 16 (APR 24 1996) JP Mcdevitt, PT Lansbury Glycosamino acids: New building blocks for combinatorial synthesis 3818-3828 Journal of Organic Chemistry 61: 8 (APR 19 1996) RS Garigipati, B Adams, JL Adams, SK Sarkar Use of spin echo magic angle spinning H-1 NMR in reaction monitoring in combinatorial organic synthesis 2911-2914 Transplantation Proceedings 28: 2 (APR 1996) DL Kooyman, SB Mcclellan, WR Parker, PL Avissar, MA Velardo, DH Nielsen, JL Platt, JS Logan Identification and characterization of a peptide mimeotope of galactose alpha 1,3 galactose 554 Genomes, Molecular Biology and Drug Discovery (1996) JC Chabala, JJ Baldwin, JJ Burbaum, D Chelsky, LW Dillard, I Henderson, G Li, MHJ Ohlmeyer, TL Randle, JC Reader, L Rokosz, NH Sigal Drug discovery and optimization using binary-encoded small molecule combinatorial libraries 131-150 Amino Acids 10: 2 (1996) TD Hua, F Lamaty, C Souriau, V Rollandfulcrand, R Lazaro, P Viallefont, MP Lefranc, M Weill Specific recognition of a tetrahedral phosphonamidate transition state analogue group by a recombinant antibody Fab fragment 167-172 Journal of Medicinal Chemistry 39: 8 (APR 12 1996) ZJ Ni, D Maclean, CP Holmes, MM Murphy, B Ruhland, JW Jacobs, EM Gordon, MA Gallop Versatile approach to encoding combinatorial organic syntheses using chemically robust secondary amine tags 1601-1608 Journal of Medicinal Chemistry 39: 8 (APR 12 1996) P Boden, JM Eden, J Hodgson, DC Horwell, J Hughes, AT Mcknight, RA Lewthwaite, MC Pritchard, J Raphy, K Meecham, GS Ratcliffe, N Sumanchauhan, GN Woodruff Use of a dipeptide chemical library in the development of non-peptide tachykinin NK3 receptor selective antagonists 1664-1675 Current Opinion in Structural Biology 6: 2 (APR 1996) GP Lomonossoff, JE Johnson Use of macromolecular assemblies as expression systems for peptides and synthetic vaccines 176-182 Proceedings of the National Academy of Sciences of the United States of America 93: 8 (APR 16 1996) JL Miller, VA Lyle Mimotope anti-mimotope probing of structural relationships in platelet glycoprotein Ib alpha 3565-3569 Analytical Biochemistry 236: 1 (APR 5 1996) JS Shin, BG Kim, DH Kim, YS Lee Spectrophotometric assay using streptavidin-alkaline phosphatase conjugate for studies on the proteolysis of polymer bead-bound peptides 9-13 Angewandte Chemie - International Edition in English 35: 6 (APR 1 1996) PA Tempest, SD Brown, RW Armstrong Solid-phase, parallel syntheses by Ugi multicomponent condensation 640-642 Angewandte Chemie - International Edition in English 35: 6 (APR 1 1996) LF Tietze, A Steinmetz Stereoselective solid-phase synthesis of cyclopentane and cyclohexane derivatives by two-component domino reactions: Generation of combinatorial libraries 651-652 Journal of the American Chemical Society 118: 14 (APR 10 1996) H Takeuchi, M Matsuno, SA Overman, GJ Thomas Raman linear intensity difference of flow-oriented macromolecules: Orientation of the indole ring of tryptophan-26 in filamentous virus fd 3498-3507 Tetrahedron 52: 15 (APR 8 1996) J Marchandbrynaert, M Bouchet, R Touillaux, C Beauve, J Fastrez Design and synthesis of a bifunctional label for selection of beta-lactamase displayed on filamentous bacteriophage by catalytic activity 5591-5606 Transplantation 61: 6 (MAR 27 1996) DL Kooyman, SB Mcclellan, W Parker, PL Avissar, MA Velardo, JL Platt, JS Logan Identification and characterization of a galactosyl peptide mimetic - Implications for use in removing xenoreactive anti-A Gal antibodies 851-855 Applied Spectroscopy 50: 4 (APR 1996) HU Gremlich, SL Berets Use of FT-IR internal reflection spectroscopy in combinatorial chemistry 532-536 From owner-repertoires@net.bio.net Wed May 22 23:00:00 1996 Path: biosci!qub.ac.uk!n.mcferran From: n.mcferran@qub.ac.uk ("N.V.McFerran") Newsgroups: bionet.molecules.repertoires Subject: http://www.bio.net:80/hypermail/MOLECULAR-REPERTOIRES/ Date: 23 May 1996 10:52:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: <199605231752.KAA28063@net.bio.net> NNTP-Posting-Host: net.bio.net I checked out the building blocks database and it works - try it. From owner-repertoires@net.bio.net Wed May 22 23:00:00 1996 Path: biosci!daresbury!yama.mcc.ac.uk!bofh.dot!news.salford.ac.uk!aber!bath.ac.uk!dcl-cs!strath-cs!queens-belfast.ac.uk!bcg0197 From: bcg0197@queens-belfast.ac.uk (Andrew Wallace) Newsgroups: bionet.molecules.repertoires Subject: Building block database website Message-ID: <1996May23.171322.6469@queens-belfast.ac.uk> Date: 23 May 96 17:13:22 GMT Organization: Queen's University of Belfast Lines: 26 This is a web page which lists about 800 commercially available compounds useful for solid-phase peptide synthesis. http://aminoacid.bri.nrc.ca:1125/ The authors claim it can be downloaded to a Mac or PC for local use. To contact the authors, use the following addresses: Author Address Topic Grzegorz Pietrzynski (pietrzyn@biotech.lan.nrc.ca) software Jacek Slon (slon@biotech.lan.nrc.ca) chemistry Yasuo Konishi (konishi@biotech.lan.nrc.ca) general Bye for now, Andrew -- ================================================================ Andrew Wallace, Ph.D. School of Biology and Biochemistry The Queen's University of Belfast Tel. +44-1232-335786 97 Lisburn Road Fax +44-1232-236505 Belfast BT9 7BL a.wallace@queens-belfast.ac.uk Northern Ireland (UK) http://www.qub.ac.uk/b&bchem/rbiochem.htm From owner-repertoires@net.bio.net Mon May 27 23:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molecules.repertoires Subject: IMPORTANT - BIOSCI Fundraising Update! Date: 28 May 1996 02:00:24 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 154 Sender: daemon@net.bio.net Distribution: world Message-ID: <199605280900.CAA04053@net.bio.net> NNTP-Posting-Host: net.bio.net BIOSCI is about halfway to its funding goal!! I'm interrupting the usual monthly posting of the BIOSCI miniFAQ to bring you up to date on BIOSCI fundraising progress, a topic of concern to your future use of this resource. Thank you in advance for taking the time to read this message carefully. Last year we announced that BIOSCI was going to adopt the U.S. Public Broadcasting System model to fund its operations after our DOE/NSF grant runs out later this year. Unlike PBS, we are not soliciting contributions from users; we are only selling ads on our Web pages solely to cover our operating costs. Our goal is to seek sponsorships until we build up an operating reserve of about $100,000 and then cease further promotions until we need to build the reserve back up. (The accountants among our readership will be familiar with the problem of deferred revenue which we can not safely utilize until ads have been displayed for a period of time.) We are only about halfway to our funding goal and need to raise further funds to avoid having to curtail services at net.bio.net. Fundraising is time-consuming, however, and we need your help as explained further below. Our operating costs consist of our network connection, phone lines, hardware maintenance (we will be getting newer and faster hardware soon!), plus 0.7 FTE of salaries covering UNIX systems admin, technical support, quality assurance, i.e., testing, of our system, and administrative costs (such as the time it takes to actually find/write/call potential sponsors and raise money!). Although the BIOSCI staff does get compensated for a portion of the work that they do, this project has always received a lot of free after-hours and "vacation" time labor, so we hope that no one will begrudge the time that we do charge to the project to serve you. All of the three part-time staff members, Dave Mack, Julie Lawrence, and myself, have full time day jobs and families in addition to working hard to keep this service running for all of you. Julie and Dave Mack are subcontractors for BIOSCI; my time that is charged to the project defrays a portion of my regular salary instead of adding to my income. Besides having to relocate the project, we were very busy this last year building new infrastructure such as our WWW hypermail interface to the system. This was released last December along with scores of WAIS indices for the newsgroups. Virtually everything is complete, although we do continue to find and fix bugs (many through your helpful feedback!). We are still having some problems with our WAIS indexing. The archives continue to grow rapidly. We are running over 100 indexes now versus three previously and any systems crashes cause greater havoc with the indexing than before! We are still working to fix this as fast as our resources permit and appreciate your patience, but we have been able to automate a lot of the infrastructure to reduce labor as compared to past requirements. We have also implemented new software to make moderation of BIOSCI/bionet newsgroups much easier and combat the growing problem of Internet junk mail and USENET "spamming." About 20% of our groups are now moderated, many of them by the BIOSCI staff! This, for example, made a major difference last year in the quality of content in our EMPLOYMENT/bionet.jobs.offered newsgroup which many commercial concerns and recruiting firms are using **without charge** to recruit candidates for positions in the biological sciences. We are also now in a position to have sponsors for individual newsgroups as you will have noticed if you have visited http://www.bio.net/ and clicked on "Access the BIOSCI/bionet newsgroups" recently. So, how can you help?? ---------------------- As noted above it can take a lot of time to contact potential sponsors if I have to do it all myself. Our request is quite simple. You can do two important things which will take very little time for you individually. First, please use our WWW system at http://www.bio.net/ to access the archives. You can now post or reply to messages via your Web browser. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. Our hope is to quickly raise several large corporate/institutional sponsors on our heavily-used WWW locations (some stats appended below), and then end this sponsorship campaign so that our resources can continue to be used for service provision, not fundraising. Many of our specialty newsgroup WWW archives are still used by small communities of scientists (and they haven't been heavily promoted yet). While these may be valuable niche markets to some advertisers, it will generate more labor and overhead having to find these sponsors, fairly price the locations, and deal with lots of smaller sponsorships than fewer mid-to large sponsors. We are striving to keep our operation as lean and efficient as possible since we are not trying to make careers out of running BIOSCI. We are trying if at all possible to avoid the administrative overhead entailed with processing lots of small payments to reach our fundraising goals. I'd like to thank all of you for your help in advance. In helping us, you are also helping yourselves, not only in keeping this resource available for all of the both large and small research communities that we serve, but also by alleviating the need for us to go back and compete with researchers for tight grant dollars! We promised NSF when we were awarded the BIOSCI grant that we would carry out this mission to make the service self-supporting. With your help, we will succeed in continuing BIOSCI's work into its second decade. Thank you very much! Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net A list of our prime WWW sponsorship locations follow. Please contact us for further details. ---------------------------------------------------------------------- The overall BIOSCI WWW pages are currently visited by users from close to 5500 unique computer hosts per week. Web servers only log the Internet computer/host name and frequently more than one individual can connect to us from a particular host. Main home page, http://www.bio.net, visited recently by about 2100 unique hosts per week Main Newsgroups archives page, http://www.bio.net/archives.html, visited recently by about 1200 Unique hosts per week BIO-JOURNALS archive page, http://www.bio.net/BIO-JOURNALS.html, visited recently by about 1000 unique hosts per week. EMPLOYMENT archive pages: http://www.bio.net:80/hypermail/EMPLOYMENT/ and monthly header pages, visited recently by about 800 unique hosts per week. Address database search page, http://www.bio.net/addrsearch.html, visited recently by about 450 unique hosts per week. Methods newsgroup archive pages, http://www.bio.net:80/hypermail/METHDS- REAGNTS/ and monthly header pages, visited recently by about 350 unique hosts per week. Ads can also be displayed on various combinations of other BIOSCI/bionet newsgroups. Please contact us at biosci-help@net.bio.net for details. ---------------------------------------------------------------------- From owner-repertoires@net.bio.net Tue May 28 23:00:00 1996 Path: biosci!mmd.com!jimostrem From: jimostrem@mmd.com ("Ostrem, Jim") Newsgroups: bionet.molecules.repertoires Subject: (none) Date: 29 May 1996 08:50:14 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <199605291546.AA16193@gate.mmd.com> NNTP-Posting-Host: net.bio.net subscribe From owner-repertoires@net.bio.net Tue May 28 23:00:00 1996 Path: biosci!daresbury!yama.mcc.ac.uk!bofh.dot!zippy.dct.ac.uk!str-ccsun!strath-cs!queens-belfast.ac.uk!bcg0197 Newsgroups: bionet.molecules.repertoires Subject: List of web (www) sites for molreps Message-ID: <1996May29.101113.6507@queens-belfast.ac.uk> From: bcg0197@queens-belfast.ac.uk (a.wallace@queens-belfast.ac.uk) Date: 29 May 96 10:11:13 GMT Organization: Queen's University of Belfast Lines: 47 Dear Molecular Librarians, Here is the latest list of web sites of interest to repertoirians: http://www.bio.net/ - The BIOSCI web site itself (MOLREPS message archive) http://bionmr1.rug.ac.be/chemistry/overview.html - A useful chemistry site http://vesta.pd.com/ - Site for the journal MOLECULAR DIVERSITY http://www.mrc-cpe.cam.ac.uk/imt-doc/vbase-home-page.html - Immunoglobulin v-gene database http://molbio.info.nih.gov/molbio/desk.html - Molecular biologists desk reference http://www.Kairos-scientific.com/ - Kairos scientific home page http://www-lmmb.ncifcrf.gov/~toms/ - Tom Schneider's Theory of Molecular Machines http://www.ebi.ac.uk/ - The European Bioinformatics Institute (EBI) http://www.ebi.ac.uk/primers_home.html - PCR primers database at EBI http://aminoacid.bri.nrc.ca:1125/ - Database of building blocks for library synthesis Keep those sites coming in! Andrew -- ================================================================ Andrew Wallace, Ph.D. School of Biology and Biochemistry The Queen's University of Belfast Tel. +44-1232-335786 97 Lisburn Road Fax +44-1232-236505 Belfast BT9 7BL a.wallace@queens-belfast.ac.uk Northern Ireland (UK) http://www.qub.ac.uk/b&bchem/rbiochem.htm From owner-repertoires@net.bio.net Thu May 30 23:00:00 1996 Path: biosci!BIOSCI.MBP.MISSOURI.EDU!petrenk From: petrenk@BIOSCI.MBP.MISSOURI.EDU (Valery Petrenko) Newsgroups: bionet.molecules.repertoires Subject: Re: Possible sponsorship Date: 31 May 1996 09:11:42 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 38 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Andrew Wallace wrote: > By the way, does anyone know if George Smith's group are > still giving out their fUSE phage libraries? I asked him for > some samples by letter about two months ago and didn't get a > reply. The phage libraries are still available. We apologize for not responding to your letter -- it must have been misplaced somehow. To receive a library, you need to send a request to George Smith by FAX to 573-882-0123 and please indicate which library (libraries) you'd like. We generally send out the library kits 2-4 weeks after receiving the request. Our kits contain: Bacteria strains: K91; K91Kan; MC1061; K802; K91BK. Vectors: f1; fuse2; fuse5; fuse3; fuse1. Libraries: pIII/6-mer, pIII/15-mer. pVIII/f88-4/15-mer. Primer for sequensing (for pIII libraries). Manual. Valery ============================================================================ ====== UNIVERSITY OF MISSOURI-COLUMBIA Valery A. Petrenko Research Professor Division of Biological Sciences 406 Tucker Hall University of Missouri Columbia, MO 65211 Office phone (314) 882-0245 Lab phone (314) 882-2720 Fax (314) 882-0123 e-mail: petrenk@biosci.mbp.missouri.edu From owner-repertoires@net.bio.net Thu May 30 23:00:00 1996 Path: biosci!daresbury!yama.mcc.ac.uk!bofh.dot!news.salford.ac.uk!aber!bath.ac.uk!dcl-cs!strath-cs!queens-belfast.ac.uk!bcg0197 From: bcg0197@queens-belfast.ac.uk (a.wallace@queens-belfast.ac.uk) Newsgroups: bionet.molecules.repertoires Subject: Possible sponsorship Message-ID: <1996May31.102854.6521@queens-belfast.ac.uk> Date: 31 May 96 10:28:54 GMT Organization: Queen's University of Belfast Lines: 21 By the way, I got a message from Clontech indicating that they might be willing to sponsor the molreps group on Biosci if we could bring the traffic on this newsgroup up a bit. It certainly seems to be very quiet here, I feel as if I'm talking to myself most of the time! Where is everybody? Too busy in the lab to be bothered with this stuff, eh? By the way, does anyone know if George Smith's group are still giving out their fUSE phage libraries? I asked him for some samples by letter about two months ago and didn't get a reply. Andrew -- ================================================================ Andrew Wallace, Ph.D. School of Biology and Biochemistry The Queen's University of Belfast Tel. +44-1232-335786 97 Lisburn Road Fax +44-1232-236505 Belfast BT9 7BL a.wallace@queens-belfast.ac.uk Northern Ireland (UK) http://www.qub.ac.uk/b&bchem/rbiochem.htm From owner-repertoires@net.bio.net Thu May 30 23:00:00 1996 Path: biosci!daresbury!yama.mcc.ac.uk!bofh.dot!news.salford.ac.uk!aber!bath.ac.uk!dcl-cs!strath-cs!queens-belfast.ac.uk!bcg0197 From: bcg0197@queens-belfast.ac.uk (a.wallace@queens-belfast.ac.uk) Newsgroups: bionet.molecules.repertoires Subject: Look at the Biosci web page! Message-ID: <1996May31.101827.6520@queens-belfast.ac.uk> Date: 31 May 96 10:18:27 GMT Organization: Queen's University of Belfast Lines: 23 If you have a web browser I strongly encourage you to look at the molecular-repertoires page on the Biosci web site. All these messages posted to molreps are automatically reformatted into nice web pages for you to look at, and any URLs in the text are turned into links you can click on. All the messages are archived by month, and you can perform a WAIS search through all the messages to find those containing any word you want. I think it's great so check it out for yourself! The URL for the Biosci home page is http://www.bio.net/ then follow the links to find the bionet.molecules.repertoires newsgroup. Andrew -- ================================================================ Andrew Wallace, Ph.D. School of Biology and Biochemistry The Queen's University of Belfast Tel. +44-1232-335786 97 Lisburn Road Fax +44-1232-236505 Belfast BT9 7BL a.wallace@queens-belfast.ac.uk Northern Ireland (UK) http://www.qub.ac.uk/b&bchem/rbiochem.htm From owner-repertoires@net.bio.net Thu May 30 23:00:00 1996 Path: biosci!pleiades.chiron.com!martine From: martine@pleiades.chiron.com (Eric Martin) Newsgroups: bionet.molecules.repertoires Subject: Too quiet? Date: 31 May 1996 14:58:58 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 39 Sender: daemon@net.bio.net Distribution: world Message-ID: <199605312147.OAA28784@pleiades.chiron.com> References: NNTP-Posting-Host: net.bio.net a.wallace@queens-belfast.ac.uk (bcg0197@queens-belfast.ac.uk) wrote: > It certainly seems to be very quiet here, OK, here is question to generate a little noise. At least two literature sources claim that the apparent "activity" of a pool is the average of association constants.<1,2> I.e., Kapp = sum([RLi])/[R]sum([Li]) = 1/N*sum(Ki), where [R] is free receptor concentration, [Li] is conc. of free ligand Li, and [RLi] is conc. of bound Li. Susan Freier, at Isis, kindly showed me that it is true for equimolar mixtures at low receptor concentration, so sum([Li]) ~ N*[Lt], where [Lt] (= [Li] + [RLi] ~ [Li]) is the constant total conc. of each ligand, and N is the number of compounds in the mixture. Is this still true if the receptor concentration is higher, so [Li] + [RLi] != [Li]? Also, what is the appropriate weighted average if the mixture is not equimolar? Cheers, Eric ------------- <1> S. M. Freier, D. A.M.Konings, J. R. Wyatt and D. J. Ecker, J. Med. Chem., 38, 344 (1995). Deconvolution of Combinatorial Libraries for Drug Discovery: A Model System. <2> M. C. Pirrung, J. Am. Chem. Soc., 117, 1240 (1995). Preparation and Screening against Acetylcholinesterase of a Non-Peptide "Indexed" Combinatorial Library. -- Eric Martin martine@chiron.com 4560 Horton St., Emeryville, CA 94608 (510)601-3306, FAX (510)601-3360 From owner-repertoires@net.bio.net Thu May 30 23:00:00 1996 Newsgroups: bionet.molecules.repertoires Path: biosci!daresbury!bioftp.unibas.ch!news.vub.ac.be!news.belnet.be!news.sri.ucl.ac.be!jussieu.fr!esiee.fr!sgigate.sgi.com!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!newsfeed.internetmci.com!lamarck.sura.net!fconvx.ncifcrf.gov!kaylor!toms From: toms@kaylor.ncifcrf.gov (Tom Schneider) Subject: Re: Possible sponsorship Message-ID: Sender: usenet@ncifcrf.gov (C News) Nntp-Posting-Host: kaylor.ncifcrf.gov Organization: Frederick Biomedical Supercomputer Center X-Newsreader: TIN [version 1.2 PL2] References: <1996May31.102854.6521@queens-belfast.ac.uk> Date: Fri, 31 May 1996 17:36:40 GMT Lines: 18 a.wallace@queens-belfast.ac.uk (bcg0197@queens-belfast.ac.uk) wrote: : It certainly seems to be very quiet here, How about putting together a FAQ and posting that once per month? People could contribute ideas to it. Kinds of repertoires currently available Methods for making repertoires Analysis of results Tom Schneider National Cancer Institute Laboratory of Mathematical Biology Frederick, Maryland 21702-1201 toms@ncifcrf.gov http://www-lmmb.ncifcrf.gov/~toms/