From owner-repertoires@net.bio.net Sun Jun 02 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!chi-news.cic.net!news.compuserve.com!news.production.compuserve.com!news From: Survey Administration <74750.1341@CompuServe.COM> Newsgroups: bionet.molec-model,bionet.molecules.peptides,bionet.molecules.repertoires,bionet.parasitology Subject: WE NEED YOUR INPUT Date: 3 Jun 1996 03:21:35 GMT Organization: Kitty Knight Corporation Lines: 25 Message-ID: <4otlnv$3ni$8@mhade.production.compuserve.com> Xref: biosci bionet.molec-model:951 bionet.molecules.peptides:333 bionet.molecules.repertoires:155 bionet.parasitology:1589 Please excuse this brief intrusion, but we are trying to locate any and all holders of academic degrees conferred within the last ten (10) years. The Kitty Knight Corporation, Boston, MA, is currently searching for qualified individuals to participate in a ***PAID*** study focusing on post-secondary, graduate, and professional education in the United States. Holders of ALL types of degrees in ALL fields of study are needed. We would like to hear from you as long as your degree was earned at an accredited institution in the United States. After an initial screening, qualified participant will be asked to complete a questionaire of approx 150 questions. Everyone who completes the survey will receive a $100 stipend. Naturally, *ALL* information will be held in the strictest confidence. To express interest, please send your name, mailing address, and photocopies of **ALL** degrees you have earned to: The Kitty Knight Corporation, Attn: Study 96-3H, Back Bay Annex, P O Box 546, Boston, MA 02117. (If not obvious from the degree, please indicate the field of study.) Thank You Very Much! From owner-repertoires@net.bio.net Tue Jun 04 23:00:00 1996 Path: biosci!daresbury!yama.mcc.ac.uk!zippy.dct.ac.uk!str-ccsun!strath-cs!queens-belfast.ac.uk!queens-belfast.ac.uk!nntp Newsgroups: bionet.molecules.repertoires Subject: Upcoming FAQ for molreps Message-ID: <1996Jun5.115805.6559@queens-belfast.ac.uk> From: "N.V.McFerran" Date: 5 Jun 96 11:58:02 GMT Nntp-Posting-Host: 104_mbc.bc.qub.ac.uk Lines: 5 Following Tom's suggestion I will soon be posting a monthly FAQ to the molreps newsgroup so watch this space... Andrew a.wallace@queens-belfast.ac.uk From owner-repertoires@net.bio.net Wed Jun 05 23:00:00 1996 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.mel.connect.com.au!harbinger.cc.monash.edu.au!lugb.latrobe.edu.au!newsmgr From: Mick Foley Newsgroups: bionet.molecules.repertoires Subject: (no subject) Date: 6 Jun 1996 00:10:39 GMT Organization: LaTrobe University Lines: 14 Message-ID: <4p57lv$mc8@lugb.latrobe.edu.au> NNTP-Posting-Host: bcmf1.biochem.latrobe.edu.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Hi all, I may have asked this of the group before, but does anyone have or know where I can get primers to make a scFv library from either hybdridomas or spleens from rats. Is there the same info on the rat repertoire anywhere. Sorry if this is a repeat, and thanks in advance for any help (primers to make rat Fabs would also be useful). Cheers Mick From owner-repertoires@net.bio.net Thu Jun 06 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: "Engle" Newsgroups: bionet.molecules.repertoires Subject: Employment Opportunity-Diversity Chem Date: 7 Jun 1996 15:24:49 +0100 Lines: 25 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4p9e3h$p0a@mserv1.dl.ac.uk> Original-To: "Molecular Repertoires" Combinatorial Chemists Vertex Pharmaceutical's expanding combinatorial chemistry group has immediate openings available for associate level chemists. Applicants must have a strong background in synthetic organic chemistry with a minimum of two years of post-bachelor degree, full time bench laboratory experience and hands-on familiarity with programmable laboratory equipment. Specific experience with solid-supported small molecule organic synthesis and/or laboratory automation equipment is a significant plus. Vertex offers a highly interactive, multidisciplinary approach with some of the best researchers in the industry. Our strong computational chemistry and informatics groups, analytical sciences, and established leadership in structure-based drug design form an exciting environment in which to integrate the tools of automated organic synthesis with the process of pharmaceutical research. Resumes may be forwarded to: Vertex Pharmaceuticals Incorporated, Recruiting Manager, Code "netchem", 130 Waverly Street, Cambridge, MA 02139-4242; FAX: (617) 577-6645; or email to: recruiting@vpharm.com We are an Equal Opportunity Employer Please visit our homepage at http://www.vpharm.com. From owner-repertoires@net.bio.net Mon Jun 10 23:00:00 1996 Path: biosci!NMSU.EDU!dkim From: dkim@NMSU.EDU ("D. KIM") Newsgroups: bionet.molecules.repertoires Subject: "Biopanning" on a membrane Date: 11 Jun 1996 09:46:36 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hello I was wondering if there was a specific reason why affinity selection (biopanning) is often done using a polystyrene well (e.g. a Petri dish or a 96-well immunoassay plate). Why is biopanning not done by binding the ligand onto a membrane such as PVDF or nitrocellulose, followed by blocking and affinity selection? Is a polystyrene well/dish better suited for some reason? Daniel Kim dkim@nmsu.edu From owner-repertoires@net.bio.net Mon Jun 10 23:00:00 1996 Path: biosci!daresbury!yama.mcc.ac.uk!zippy.dct.ac.uk!str-ccsun!strath-cs!queens-belfast.ac.uk!bcg0197 Newsgroups: bionet.molecules.repertoires Subject: Re: (no subject) - rat primers Message-ID: <1996Jun11.102107.6617@queens-belfast.ac.uk> From: bcg0197@queens-belfast.ac.uk (a.wallace@queens-belfast.ac.uk) Date: 11 Jun 96 10:21:07 GMT References: <4p57lv$mc8@lugb.latrobe.edu.au> Organization: Queen's University of Belfast Lines: 28 In article <4p57lv$mc8@lugb.latrobe.edu.au>, Mick Foley writes: > Hi all, > > I may have asked this of the group before, but does anyone have or know > where I can get primers to make a scFv library from either hybdridomas or > spleens from rats. Is there the same info on the rat repertoire anywhere. > Sorry if this is a repeat, and thanks in advance for any help > (primers to make rat Fabs would also be useful). > > Cheers > > Mick > > > I suppose you could try asking Greg Winter (I think his address is gw@mrc-lmb.cam.ac.uk) but he may only have the primers for mouse (as would most people, I suspect). Andrew -- ================================================================ Andrew Wallace, Ph.D. School of Biology and Biochemistry The Queen's University of Belfast Tel. +44-1232-335786 97 Lisburn Road Fax +44-1232-236505 Belfast BT9 7BL a.wallace@queens-belfast.ac.uk Northern Ireland (UK) http://www.qub.ac.uk/b&bchem/rbiochem.htm From owner-repertoires@net.bio.net Tue Jun 11 23:00:00 1996 Path: biosci!daresbury!yama.mcc.ac.uk!news.salford.ac.uk!aber!bath.ac.uk!dcl-cs!strath-cs!queens-belfast.ac.uk!queens-belfast.ac.uk!nntp Newsgroups: bionet.molecules.repertoires Subject: New epitope tag Message-ID: <1996Jun12.105059.6626@queens-belfast.ac.uk> From: Andrew Wallace Date: 12 Jun 96 10:50:59 GMT Nntp-Posting-Host: 104_mbc.bc.qub.ac.uk Lines: 10 A while ago, Andrew Bradbury asked about novel epitope tags. I have seen one in a recent issue of Gene which may be useful. It is derived from the VP7 major core protein of bluetongue virus and the sequence is QYPALT in single-letter code. The authors call it BTag. Reference: Wang, L-F. et al., Gene 169 (1996) 53-58. Andrew a.wallace@queens-belfast.ac.uk From owner-repertoires@net.bio.net Tue Jun 11 23:00:00 1996 Path: biosci!daresbury!yama.mcc.ac.uk!news.salford.ac.uk!aber!bath.ac.uk!dcl-cs!strath-cs!queens-belfast.ac.uk!queens-belfast.ac.uk!nntp Newsgroups: bionet.molecules.repertoires Subject: Braunschweig meeting Message-ID: <1996Jun12.114923.6629@queens-belfast.ac.uk> From: Andrew Wallace Date: 12 Jun 96 11:49:22 GMT Nntp-Posting-Host: 104_mbc.bc.qub.ac.uk Lines: 7 Could someone who has a copy of the Braunschweig libraries meeting in electronic form please post it here? Thanks, Andrew From owner-repertoires@net.bio.net Tue Jun 11 23:00:00 1996 Path: biosci!daresbury!yama.mcc.ac.uk!news.salford.ac.uk!aber!bath.ac.uk!dcl-cs!strath-cs!queens-belfast.ac.uk!bcg0197 From: bcg0197@queens-belfast.ac.uk (a.wallace@queens-belfast.ac.uk) Newsgroups: bionet.molecules.repertoires Subject: Molreps FAQ for June 1996 Message-ID: <1996Jun12.113406.6627@queens-belfast.ac.uk> Date: 12 Jun 96 11:34:06 GMT Organization: Queen's University of Belfast Lines: 134 Here is this month's edition (the first) of the molreps FAQ. You are all hereby cordially invited to send me corrections, particularly if something is not true or outdated, comments, flames (but not too many I hope), etc. Any additional information or questions you would like to see included are especially welcome. My thanks to Franco Felici and Tom Schneider for their help in preparing this first version. =================================================================== M O L R E P S F R E Q U E N T L Y A S K E D Q U E S T I O N S ************* ******************* ********* ***************** **1 Where can I obtain libraries?** 1.1 _Phage Libraries_ You can try approaching the following people and organisations: 1.1.1 pIII/6-mer, pIII/15-mer, pVIII-4/15-mer libraries George Smith, University of Missouri, FAX +1-573-882-0123 1.1.2 pVIII/9-mer, pVIII/9-merCys and pVIII/zinc-finger phagemid libraries Alessandra Luzzago, IRBM P. Angeletti, luzzago@irbm.it Franco Felici, IRBM P. Angeletti, felici@irbm.it (NON-COMMERCIAL USERS ONLY) 1.1.3 Antibody scFvs with synthetic CDRs Greg Winter, MRC-LMB Cambridge, gw@mrc-lmb.cam.ac.uk (NON-COMMERCIAL USERS ONLY) 1.1.4 Commercial Sources Stratagene (Fab fragments in phage lambda) Affymax Pharmacia Cambridge Antibody Technology 1.2 _Synthetic Peptide Libraries_ 1.2.1 Commercial Sources Affymax Selectide Chiron Corporation Most of the major peptide companies will custom-synthesise a library to your requirements. 1.3 _Nucleic Acid Libraries_ (Aptamers) 1.3.1 Jack Szostak at Harvard medical school? 1.3.2 NEXUS corporation (Larry Gold)? 1.4 _Organic Chemical Libraries_ 1.4.1 Affymax? 1.4.2 Parke-Davis (DIVERSOMERS)? **2) Where can I get anti-phage antibodies?** 2.1 anti-pIII MAb from Michael Tesar mte@venus.gbf-braunschweig.d400.de 2.2 anti-pIII polyclonals from GATC, a German company, FAX +49-7531-57313 TEL +49-7531-57204 2.3 rabbit anti-M13 from Stratagene. **3) How can I make my own libraries?** 3.1 _Phage Libraries_ You can obtain suitable vectors and strains from most of the sources in 1). For phagemid work you can get XL-1 Blue cells from Stratagene and M13K07 helper phage from Pharmacia. 3.2 _Synthetic Peptide Libraries_ 3.2.1 Manual synthesis Houghten's "Tea Bag" method Geysen's "Pin" method Both of these can be adapted to produce either support-bound or soluble libraries. 3.2.2 Automated synthesis Chiron Corporation (Zuckermann) Advanced Chemtech (Commercial robot for 75K sterling) 3.3 _Nucleic Acid Libraries_ 3.3.1 PCR methods 3.4 _Organic Chemical Libraries_ 3.4.1 Manual synthesis 3.4.2 Automated synthesis (Advanced Chemtech) **4) How can I analyse the results of my selection?** 4.1 Insert sequencing (phage libraries) 4.2 Micropanning (Smith and Scott, Methods Enzymol. (1993) 217:228-257) 4.3 Dot-blotting (Felici et al., J. Mol. Biol. (1991) 222:301-310) 4.4 ELISA (Smith and Scott, Methods Enzymol. (1993) 217:228-257) (Dente et al. Gene (1994) 148:7-13) 4.5 Colony immunoscreening (Christian et al. J. Mol. Biol. (1992) 227, 711-718) (Felici et al. Gene (1993) 128, 21-27) 4.5 Plaque immunoscreening (Luzzago et al., Gene (1993) 128:51-57) (Felici et al., Methods Enzymol. (1996) 267:116-129) -- ================================================================ Andrew Wallace, Ph.D. School of Biology and Biochemistry The Queen's University of Belfast Tel. +44-1232-335786 97 Lisburn Road Fax +44-1232-236505 Belfast BT9 7BL a.wallace@queens-belfast.ac.uk Northern Ireland (UK) http://www.qub.ac.uk/b&bchem/rbiochem.htm From owner-repertoires@net.bio.net Thu Jun 13 23:00:00 1996 Path: biosci!rutgers!sgigate.sgi.com!news-res.gsl.net!news.gsl.net!nntp.coast.net!oleane!jussieu.fr!news.sri.ucl.ac.be!news.belnet.be!swsbe6.switch.ch!scsing.switch.ch!rzunews.unizh.ch!newsadm From: simone@erdw.ethz.ch Newsgroups: bionet.molecules.repertoires Subject: Nissim scFv phage display library Date: Fri, 14 Jun 1996 13:49:14 +0200 Organization: University of Zurich, Switzerland Lines: 3 Message-ID: <31C151BA.7AAA@erdw.ethz.ch> NNTP-Posting-Host: 130.60.168.115 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win95; I) Has anybody got any experience with the Nissim scFv library? So far we have only heard of good results, but obviously it is usually more difficult to find information of people who have had difficulties. From owner-repertoires@net.bio.net Thu Jun 13 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: EMBO Course on phage display Date: 14 Jun 1996 15:20:00 +0100 Lines: 243 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4prseg$cie@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk This attachment is a binhex-encoded document containing the application for for Andrew Bradbury's phage display EMBO course in September. 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J)#!J#9"S,N3Z)(0dG@4PER3J)#!J)!d06Q&dD@pZB@aTG(N*#3N*)#!J+'ePE@* PFLNJ48e#6b!J)#!J)#!J)#!J)#!J)#!J580(48)J)#!J$3e5CA0PBA*MD#"PH(" PFQPPEQ0P$3d0$3d0$3d0$3e8DA4XC5"[CL"dD'8JG'9Z)'eTER9dC5"dB@aV)(P [G5"hD@aX)'GTGQ80$3e)EhFJGfPXE#"dD'Pc)'0[GA*cC5"LC@jPCQPd)(P[GA) JFQ9cC@&bBfJr$3d0$3d0$3d09fKKG#"jEh8JGfPXE#"LC5"KBQaP)(4[)'pQCQ9 b)'pdD'9b)'0[GA*cC5"YC@eLCA*c2`d0$3d0$3e8GfmJBQ9cG#"`G@*XD@0KG'P [ER-0$3d0$3eAEh9XC#"jEh8JE'PVC5"dEb"LFQPZCb"K)(0KEA"XC6mJB@jN)'P Q)(0[)(GSBA3JDA-JG'KP)(0KEA"XC5"jEh8JGfpeE'3JE'PVC5"dEb"LFQPZCcm 0$3d0$3d0$4&e!(J#iAX#iA`'TB-#iB3#i3!"#J$J!,B!M!"L!$J!$J!!!3!!!"a !!!!F5!!!(Gd!!"hH!!!Gh`!!(I%!r3!!!2F!!!!!!!!!!!!!!!!!!!!!!!!!!!! !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! !!!!!!!!!#J"!!!)B!!!!!"J!"!!3!!-'!!!"!!!!!3%!!!%#!!!"%J!!!4-!!!' 1!!!"aJ!!!FF!!!(N!!!"j3!!!HB!!!(R!!!"k!!!!HN!!!(U!!!"k`!!!H`!!!( m!!!#-3!!!M)!!!5U!!!%U`!!",i!!!6+!!!%i`!!"4`!!!8G!!!&P3!!"K)!!!B 6!!!'(`!!"Ym!!!G[!!!([3!!"li!!!JC!!!)'J!!$*B!!!bA!!!-S`!!$1i!!!f !!!!0[3!!$Ei!!!h%!!!1M`!!$YN!!!lD!!!1i3!!$pS!!!rE!!!2m3!!%!)!!"& +!!!5M!!!%`%!!"-q!!!62`!!%dN!!"4P!!!8MJ!!&3%!!"86!!!9J!!!&CS!!"A Fl1cXl0MXl1cXl1cXl1cXl1c6cXR1cXl1cXl%a-krZX61cX61YFkrcX61cV#kcXk rZXkV[kDQa-l1[kE1a-l%cXi!!!!%!!!%)&J!$!!!"2m!!5"B!!m!!!6q!!!!!!! !!!!%!!!2)&J!$!!!"!!!!b"B!!`!!!6q!!%J@!!-!!!%!!!#)&J!$!!!"!!!##" B!!`!!!3!!!%J@!!-!!!%r`!!!!!!!!!!%`!!!L"B!!`3rTJ2'J!)!Y!(#!U-$Q! 55"BF'J3Hm!!!!!!!!!!J!"-!!!%J@!!-%2kB$aS!#!,3"`J+M!jJ%NJ@("S%([! !!!!!!!!!)%%!!"AF!!!9h3!!&I3!!"Dj!!!@c3!!&`F!!"GD!!!A@`!!&i)!!"Q P!!!CTJ!!'E`!!"Sl!!!E9`!!'iX!!"Z-!!!Ek`!!'q`!!"`)!!!F#3!!(!S!!"` ,!!!F$!!!(#B!!"`R!!!FE`!!((!!!"bU!!!FU`!!(,m!!"c!!!!F`3!!(-)!!"c $!!!Fa!!!(-8!!"c'!!!Fa`!!(-J!!"c*!!!Fp!!!(28!!"cf!!!G)J!!(5-!!"d N!!!G*3!!(5B!!"dR!!!G+!!!(5N!!"dU!!!GA`!!(@!!!"eK!!!GBJ!!(@-!!"e N!!!GC3!!(AX!!"em!!!GI3!!(Ai!!"er!!!GJ!!!(GJ!!"hC!!!GfJ!!(GX!!"h F!!!Gh3!!(Gi!!"hIqrEaqr[lqrEXqrERiYhljr[lqr[lqr[lqr[lqr[lqr[lqr[ lqr[lqr[lqr[lqr[lqr[lqr[lqr[lqr[lqr[lqqIlqr[lbF6*!!!!!!!!!!!!!!! !!!!!!!!!!!!!!!!!!!!!!!3!!!!!!!!!!!!6!!!")&J!$"$qQ!mD!!J#d!F)#S` 1B"*)&K`D""l`!!!!!!!!!#!!"2m!!!!!!!!!!!3!!!3J@!!-!!!%!!!#)&J!$!! !"!!!"b"B!!`!!!3!!!-J@!!-!!!%rJ!")&J!$!!!"!!!!5"B!!`!5!!1!"%!!!$ rrrrrrrrrr`!!!!!!(2rrrrrrrrrrrrrr!B!"J!@!#!!!(!3!%!!8!$2rrrrrrrr rrrrrr`Ip!!!!!!!!#[i!!!!!!!!9!(J+r`!!!!!!!"8!m!F!!!!!!!!!!!m!!2- !p!!!!2B!!!!!!!!!!!!!!!!!!!!!!!!!!0i!!!!!!!!Fh`!%!!!Gh`!!!!!!!!$ p!!!-[`!!'#F!!"TB!!!FfJ!!(0m3!2rr!!!!!3!d!!$rr`!!!!)!"4!!rrm!!!! $!(i!!2rr!!!!"!!c%!$rr`!!!!8!!3!!rrm!!!!'!!!!!!!!!!!!!`!!!3!!!"h a!!m!!!%!!!!9h!!!(Gm!%!!4!!!!!J!$!!3!$3!3!")!&!!9!"B!&`!K"!!(d!I A"q%(k!IY"r3(pJIi"ri)"!R61GXkIJ!"!!!!5!")!!!!!!0)!N!!!2rh!dJ#5BN '"AX$hJ!!!!!!f!$B!!!!!!RB"X!!f!!J!'3E!!"2!!%"!3!!!!%R$`!"!!%!!!! !!!!!!!!!!!!!"BN'!'3!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!%' d,QJ&S!F)"I!(#!!!!Y"#!!!"!!%!!8!!!!!!!!")!"8-,6T'BAJJ8f9ZC'9b!!S !!!!!!!)!!!!$"NGPEQ9fB3!!!"3&9'PYCA1!!3%!!!!-[`!!$,m!#)!!J!!!!!b r!!!!!!!S!!)"K3)H!1B!!J'3!!$r!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! !!!!!!!!!!!!!0*m!!!: --============_-1377532728==_============ Content-type: text/plain; charset="us-ascii" Andrew Bradbury SISSA c/o ICGEB Area di Ricerca Padriciano 99 Trieste 34012 Italy Tel +39 40 398995 Fax +39 40 398991 --============_-1377532728==_============-- From owner-repertoires@net.bio.net Thu Jun 13 23:00:00 1996 Path: biosci!EMAIL.UNC.EDU!briankay From: briankay@EMAIL.UNC.EDU (Brian Kay) Newsgroups: bionet.molecules.repertoires Subject: phage display manual Date: 14 Jun 1996 06:22:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 36 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hi All, Jill Winter (Chiron), John McCafferty (CAT), and I are pleased to announce that the book, "Phage display of peptides and proteins: a laboratory manual", is in production at Academic Press. If you wish to peruse the table of contents, I have created a webpage companion to the book. Its address is http://www.unc.edu/depts/biology/phagedisplay.html Only ~1/3 of the linked pages are complete. The book will be a soft-cover manual, spiral bound, with ~330 pages. It costs $39.95 and can be oredred by fax (1-800-874-6418) or telephone (1-800-321-5068) from Academic Press. The ISDN is 0-12-402380-0. Sincerely yours, Brian ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Brian Kay Associate Professor of Biology 321 Fordham Hall University of North Carolina at Chapel Hill Chapel Hill, NC 27599-3280 Office: 919-962-2144 Fax: 919-962-1625 Email: briankay@email.unc.edu Webpage: http://www.unc.edu/depts/biology/kay.html Phage Display Book: http://www.unc.edu/depts/biology/phagedisplay.html ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From owner-repertoires@net.bio.net Sun Jun 16 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Braunschweig Symposium Date: 17 Jun 1996 11:49:22 +0100 Lines: 166 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4q3d7i$1t7@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk For details about the Braunschweig Symposium, consult the URL http://transfac.gbf-braunschweig.de/Symposien/Welcom2.html Here is some of the information you will find there: > GBF-SYMPOSIUM > > Antibody Technology and Applications in > Health and Environment > > > September 6 - 10, 1996 > in Braunschweig, Germany > > Goals and Scope > The goals of the Symposium are to present latest advances in the field > of antibody technology with regard to emerging innovative approaches > and applications in health and environment. During the first part of > the symposium research progress will be presented in the fields of > natural immune repertoires, antibody structure and function and > recombinant antibody technology including human and humanized > antibodies, phage display techniques, combinatorial libraries and > bispecific or catalytic antibodies. In the second part, potentials and > problems of antibody expression in cell cultures, transgenic animals > and plants will be discussed with regard to the antibody application > sessions in the third part of the Symposium. The final sessions will > analyze the use of antibody for prophylaxis and therapy and in a > variety of other fields with emphasis on the environment. Interactions > will be encouraged during round-table sessions where potential uses, > common difficulties and limitations of antibody technology will be > discussed. Additional time for discussions will be available during > poster sessions, social activities and informal periods of time in the > Hotel Mercure where most of the participants will be accommodated. > Younger scientiests will have the opportunity to submit abstracts for > short oral presentations of selected posters in the main sessions. > ------------------------------------------------------------------------ > > > Main topics of the symposium and confirmed speakers: > > Antibodies: Natural Repertoires, Structure and Function > A. Coutinho (France), A. McPherson (USA), A. > Skerra (Germany) > > Antibody Engineering > [Image]Display Technology > A. Bradbury (Italy), A. Griffith (U.K.), M. > Little (Germany), P.-E. Nygren (Sweden), > A. Pl|ckthun (Switzerland), G.P. Smith (USA) > > [Image]Combinatorial Libraries > J. Collins (Germany), R. Cortese (Italy), R. > Sodoyer (France), A. Williamson (USA), B. Kay > (USA) > > [Image]Human and Humanized Antibodies > N. Lonberg (USA) > > [Image]Bispecific and Catalytic Antibodies > P. Holliger (U.K.), G. Gallacher (U.K.), P. > Carter (USA) > > Expression of MAbs and Tag Systems > B. Brizzard (USA), B. Haase (Germany), A. > Depicker (Belgium), H. Meade (USA), H. Kvhler > (USA) > > Medical Applications > [Image]Diagnostic and Imaging > A. Wu (USA), W. Wels (Germany); R. Miller > (USA) > > [Image]Therapie and Prophylaxis > M. Bendig (UK), G.S. Chhatwal (Germany), S.J. > Cryz (Switzerland), > F. Emmrich (Germany), J. Engberg (Denmark), > H. Lindhofer (Germany), J.R. McGhee (USA) > > Environmental Applications > J. Kvhl (Germany), E. Conway de Macario > (USA), M. Tesar (Germany), B. Harris (UK) > P.V. Choudary (USA) > ------------------------------------------------------------------------ > > > If you would like to receive more information, please submit the fill > out form: > > First Name > Last Name and Title > Organization > Department > Address > City > ZIP/Postal Code > State/Country > Telephone > Fax > E-Mail > Comments > > > > > > You could also reach us at the following address: > You could also reach us at the following address: > [Greetings] > GBF-Braunschweig > Antibody Symposium > Department Microbiology/Cell Biology > Mascheroder Weg 1 > D - 38124 Braunschweig > Germany > Tel: +49 531 6181 400 (H. Brink) > Fax: +49 531 6181 411 > > > or directly via E-Mail: > [Image]Antibody Symposium at the GBF-Braunschweig [Image] > > ------------------------------------------------------------------------ > > > Organizers: Gusharan S. Chhatwal, Bernd Haase, Michael Tesar, Carlos > A. Guzman, Kenneth N. Timmis > From owner-repertoires@net.bio.net Sun Jun 16 23:00:00 1996 Path: biosci!qub.ac.uk!a.wallace From: a.wallace@qub.ac.uk (Andrew Wallace) Newsgroups: bionet.molecules.repertoires Subject: EMBO Course - Date Correction. Date: 17 Jun 1996 04:39:17 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: <199606171139.EAA09730@net.bio.net> NNTP-Posting-Host: net.bio.net Looks like I've got my dates wrong again - the phage display EMBO course is in November, not September as I said in my previous message on this topic. Andrew "Ohh Dearie Mee" as they say in "The High Life" P.S. When is "The Comedy Zone" going to put on some new shows instead of repeats from 1990? From owner-repertoires@net.bio.net Tue Jun 18 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: "Janet Clench, Library, Tel:(39 6)91093220" Newsgroups: bionet.molecules.repertoires Subject: It's been a long time comin', but let's hope it's worth it ... Date: 19 Jun 1996 11:38:06 +0100 Lines: 204 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4q8lae$qre@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk ********************************************************* SUBJECT: Combinatorial & Phage Libraries DATE: May 27, June 3,10,17, 1996 ******************************************************** Biotechniques 20: 5 (MAY 1996) P Reddy, K Mckenney Improved method for the production of M13 phage and single-stranded DNA for DNA sequencing 854 Tetrahedron 52: 20 (MAY 13 1996) E Leikauf, F Barnekow, H Koster A combinatorial protecting group strategy for oligonucleotide synthesis 6913-6930 Tetrahedron 52: 20 (MAY 13 1996) T Lescrinier, R Busson, J Rozenski, G Janssen, A Vanaerschot, P Herdewijn Incorporation of 5-hydroxytryptophan in oligopeptides 6965-6972 Tetrahedron Letters 37: 19 (MAY 6 1996) J Nielsen, PH Rasmussen Implementation of a combinatorial cleavage and deprotection scheme .1. Synthesis of phthalhydrazide libraries. 3351-3354 Antimicrobial Agents and Chemotherapy 40: 5 (MAY 1996) SE Blondelle, E Perezpaya, RA Houghten Synthetic combinatorial libraries: Novel discovery strategy for identification of antimicrobial agents 1067-1071 European Journal of Immunology 26: 3 (MAR 1996) RMT Dewildt, R Finnern, WH Ouwehand, AD Griffiths, WJ Vanvenrooij, RMA Hoet Characterization of human variable domain antibody fragments against the U1 RNA-associated A protein, selected from a synthetic and a patient-derived combinatorial V gene library 629-639 Molecular Biology 30: 1 Part 1 (JAN-FEB 1996) AA Ilichev, NV Melamed, IA Razumov, AZ Maksyutov, AV Pereboev, AI Zakabunin, VB Loktev Expression of gene fragments for the E2 protein of Venezuelan equine encephalomyelitis virus in a phage T7 RNA polymerase-based system 46-50 Proceedings of the National Academy of Sciences of the United States of America 93: 9 (APR 30 1996) YM Zhao, TW Muir, SBH Kent, E Tischer, JM Scardina, BT Chait Mapping protein-protein interactions by affinity- directed mass spectrometry 4020-4024 Basic DNA and RNA Protocols (Series: Methods in Molecular Biology 58 (1996)) GZ Yu Cloning into M13 bacteriophage vectors 343-348 Basic DNA and RNA Protocols (Series: Methods in Molecular Biology 58 (1996)) FM Tomley M13 phage growth and single-stranded DNA preparation 359-362 Plant Cell 8: 4 (APR 1996) MNV Williams, G Freshour, AG Darvill, P Albersheim, MG Hahn An antibody Fab selected from a recombinant phage display library detects deesterified pectic polysaccharide Rhamnogalacturonan II in plant cells 673-685 Journal of Biological Chemistry 271: 18 (MAY 3 1996) P Steinberger, D Kraft, R Valenta Construction of a combinatorial IgE library from an allergic patient - Isolation and characterization of human IgE Fabs with specificity for the major timothy grass pollen allergen, Phl p 5 10967-10972 Clinical Immunology and Immunopathology 79: 2 (MAY 1996) M Sioud, O Forre, A Dybwad Selection of ligands for polyclonal antibodies from random peptide libraries: Potential identification of (auto)antigens that may trigger B and T cell responses in autoimmune diseases 105-114 International Archives of Allergy and Immunology 110: 1 (MAY 1996) R Crameri, K Blaser Cloning Aspergillus fumigatus allergens by the pJuFo filamentous phage display system 41-45 Journal of Immunology 156: 10 (MAY 15 1996) M Eggena, SR Targan, L Iwanczyk, A Vidrich, LK Gordon, J Braun Phage display cloning and characterization of an immunogenetic marker (perinuclear anti-neutrophil cytoplasmic antibody) in ulcerative colitis 4005-4011 Science 272: 5266 (MAY 31 1996) RF Service Chemistry - Combinatorial chemistry hits the drug market 1266-1268 Scientist 10: 10 (MAY 13 1996) KS Brown Drug, biotech firms beginning to embrace combinatorial chemistry 1 Journal of Medicinal Chemistry 39: 10 (MAY 10 1996) JM Gao, XH Cheng, RD Chen, GB Sigal, JE Bruce, BL Schwartz, SA Hofstadler, GA Anderson, RD Smith, GM Whitesides Screening derivatized peptide libraries for tight binding inhibitors to carbonic anhydrase II by electrospray ionization mass spectrometry 1949-1955 Journal of Immunology 156: 11 (JUN 1 1996) C Prezzi, M Nuzzo, A Meola, P Delmastro, G Galfre, R Cortese, A Nicosia, P Monaci Selection of antigenic and immunogenic mimics of hepatitis C virus using sera from patients 4504-4513 Nature Biotechnology 14: 3 (MAR 1996) TJ Vaughan, AJ Williams, K Pritchard, JK Osbourn, AR Pope, JC Earnshaw, J Mccafferty, RA Hodits, J Wilton, KS Johnson Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library 309-314 Nature Biotechnology 14: 3 (MAR 1996) MA Alvarez, H Fu, C Khosla, DA Hopwood, JE Bailey Engineered biosynthesis of novel polyketides: Properties of the whiE aromatase/cyclase 335-338 Nature Biotechnology 14: 4 (APR 1996) AM Wu In vivo veritas: Live phage display panning 429-431 Nature Biotechnology 14: 4 (APR 1996) D Neri, H Petrul, G Winter, Y Light, R Marais, KE Britton, AM Creighton Radioactive labeling of recombinant antibody fragments by phosphorylation using human casein kinase II and [gamma-P-32]-ATP 485-490 Nature Biotechnology 14: 4 (APR 1996) DM Evans, KP Williams, B Mcguinness, G Tarr, F Regnier, N Afeyan, S Jindal Affinity-based screening of combinatorial libraries using automated, serial-column chromatography 504-507 Journal of Molecular Biology 259: 1 (MAY 31 1996) H Zhang, MM Skinner, WS Sandberg, AHJ Wang, TC Terwilliger Context dependence of mutational effects in a protein: The crystal structures of the V351, 147V and V351/147V gene V protein core mutants 148-159 Journal of Molecular Biology 258: 5 (MAY 24 1996) LLC Bonnycastle, JS Mehroke, M Rashed, X Gong, JK Scott Probing the basis of antibody reactivity with a panel of constrained peptide libraries displayed by filamentous phage 747-762 Proceedings of the National Academy of Sciences of the United States of America 93: 10 (MAY 14 1996) P Kast, M Asifullah, N Jiang, D Hilvert Exploring the active site of chorismate mutase by combinatorial mutagenesis and selection: The importance of electrostatic catalysis 5043-5048 From owner-repertoires@net.bio.net Wed Jun 19 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Franco Felici Newsgroups: bionet.molecules.repertoires Subject: Methods in Enzymology - Volume 267 Date: 20 Jun 1996 09:47:07 +0100 Lines: 134 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4qb36b$sdh@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk Some of you may find the following book interesting... All the best, Franco ----------------------------------------------------------- Methods in Enzymology Volume 267 Combinatorial Chemistry EDITED BY John N. Abelson ACADEMIC PRESS, 1996 San Diego New York Boston London Sydney Tokyo Toronto Table of Contents ================= Section I. Phage Display Libraries 1. Affinity Maturation of Phage-Displayed Peptide Ligands by J. YU and G.P. SMITH 2. Selection for Protease Inhibitors Using Bacteriophage Display by W. MARKLAND et al. 3 Phage Display of Proteases and Macromolecular Inhibitors by CHENG-I WANG et al. 4. Affinity Maturation of Proteins Displayed on Surface of M13 Bacteriophage as Major Coat Protein Fusions by B.L. ROBERTS et al. 5. Screening of Phage Antibody Libraries by J.L. HARRISON et al. 6. Immunization with Phage-Displayed Mimotopes by G. GALFRE' et al. 7. Phage-Displayed Peptides as Tools for Characterization of Human Sera by F. FELICI et al. 8. Phage Display Methods for selecting Zinc Finger Proteins with DNA-Binding Specificities by E.J. REBAR et al. 9. In Vitro Genetic Analysis of RNA-Binding Proteins Using Phage Display Libraries by I.A. LAIRD-OFFRINGA and J.G. BELASCO Section II. lac Repressor Fusion Proteins 10. Screening of Peptide Libraries Linked to lac Repressor by P.J. SCHATZ et al. Section III. Libraries on Polysomes 11. Cell-Free Synthesis of Peptide Libraries Displayed on Polysomes by L.C. MATTHEAKIS et al. Section IV. Peptide Libraries 12. One Bead-One Compound Combinatorial Peptide Library: Different Types of Screening by C.L. CHEN et al. 13. Generation and Use of Nonsupport-Bound Peptide and Peptidomimetic Combinatorial Libraries by J.M. OSTRESH et al. 14. Combinatorial Chemistry: A Liquid-Phase Approach by K.D. JANDA and H. HAN 15. Preparation of Equimolar Mixtures of Peptides by Adjustment of Activated Amino Acid Concentrations by K.M. IVANETICH and D.V. SANTI 16. Encoded Combinatorial Chemistry: Binary Coding Using Chemically Robust Secondary Amine Tags by ZHI-JIE NI et al. Section V. Nucleic Acid Libraries 17. A SELEX Primer by T. FITZWATER and B. POLISKY 18. Identifying Consensus Patterns and Secondary Structure in SELEX Sequence Sets by J.P. DAVIS et al. 19. Affinity Selection-Amplification from Randomized Ribooligonucleotide Pools by J. CIESIOLKA et al. 20. In Vitro Selection of Nucleic Acid Aptamers That Bind Proteins by R.C. CONRAD et al. 21. Randomization and Selection of RNA to Identify Targets for RRM RNA-Binding Proteins and Antibodies by J.D. KEENE 22. Selection of Aminoacylated tRNAs from RNA Libraries Having Randomized Acceptor Stem Sequences: Using Old Dogs to Perform New Tricks by J.R. SAMPSON and M.E. SARKS 23. In Vitro Evolution of Randomized Ribozymes by J. TSANG and G.F. JOYCE 24. Peptide Nucleic Acids: A New Dimension to Peptide Libraries and Aptamers by P.E. NIELSEN Section VI. Other Small Molecule Libraries 25. Synthesis of N-Substituted Glycine Peptoid Libraries by G.M. FIGLIOZZI et al. 26. Synthesis and Evaluation of 1,4-Benzodiazepine Libraries by B.A. BUNIN et al. From owner-repertoires@net.bio.net Wed Jun 19 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Mirror Image Phage Date: 20 Jun 1996 18:48:50 +0100 Lines: 30 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4qc2u2$an1@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk What do you think of the recent paper in Science where they used L-amino acid-bearing phage against a synthetic D-aa protein ligand, took the binding sequence, synthesised a retro peptide sequence of D-aas and found that it bound to the corresponding L-aa protein? Sounds confusing, doesn't it. They seem to be saying that the ligand ACDEFGH binding to a site on a D-aa receptor will be the mirror image situation to peptide hgfedca (using lower case for D-aas) binding to the corresponding site on an L-aa receptor. Does this make sense? What does this imply for the peptides, would it be true that ACDEFGH and hgfedca would adopt the same 3D structure but bind in opposite orientations, perhaps? Andrew ================================================================== Andrew Wallace, Ph.D., Queen's University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://www.qub.ac.uk/b&bchem/rbiochem.htm From owner-repertoires@net.bio.net Wed Jun 19 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Re: Methods in Enzymology - Volume 267 Date: 20 Jun 1996 18:19:00 +0100 Lines: 27 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4qc164$8f3@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk >----------------------------------------------------------- > >Methods in Enzymology > >Volume 267 > >Combinatorial Chemistry > >EDITED BY >John N. Abelson > >ACADEMIC PRESS, 1996 >San Diego New York Boston London Sydney Tokyo Toronto > Looks good, does anyone know if it is possible to order individual titles from the Methods in Enzymology series? I would have thought it is but the library/bookshop here at QUB told me that Academic Press wouldn't allow it! (seems odd to me). Andrew ================================================================== Andrew Wallace, Ph.D., Queen's University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://www.qub.ac.uk/b&bchem/rbiochem.htm From owner-repertoires@net.bio.net Thu Jun 20 23:00:00 1996 Path: biosci!rutgers!sgigate.sgi.com!esiee.fr!jussieu.fr!news.sri.ucl.ac.be!news.belnet.be!swsbe6.switch.ch!scsing.switch.ch!rzunews.unizh.ch!newsadm From: simone@erdw.ethz.ch Newsgroups: bionet.molecules.repertoires Subject: Re: Methods in Enzymology - Volume 267 Date: Fri, 21 Jun 1996 10:52:10 +0200 Organization: University of Zurich, Switzerland Lines: 2 Message-ID: <31CA62BA.3920@erdw.ethz.ch> References: <4qb36b$sdh@mserv1.dl.ac.uk> NNTP-Posting-Host: 130.60.168.115 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win95; I) Does anybody know when volume 267 is going to be out? (Our library has only got up to volume 264) From owner-repertoires@net.bio.net Thu Jun 20 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Franco Felici Newsgroups: bionet.molecules.repertoires Subject: Re: Methods in Enzymology - Volume 267 Date: 21 Jun 1996 10:46:18 +0100 Lines: 17 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4qdr1a$efv@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk Well, I have a copy of the above Volume in my hands... We have received few days ago a complimentary copy from the publisher. I guess that it should be very soon available (if not already). You could ask directly: Academic Press, Inc. 6277 Sea Harbor Drive, Orlando, FL 32887 USA Tel. 407-345-2000 Fax: 407-345-4058 or Academic Press, Inc. 525 B Street, Suite 1900, San Diego CA 92101-4495 USA or Academic Press Limited, 24-28 Oval Road, London NW1 7DX England Best wishes, Franco Felici From owner-repertoires@net.bio.net Sun Jun 23 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: RE: Mirror Image Phage Date: 24 Jun 1996 17:05:02 +0100 Lines: 29 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4qmebe$q25@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk Hmmm....Yes, I think AndrewB is right, the authors of the Science paper said that ACDEF bound to D-aa receptor so acdef will bind to L-aa receptor, not fedca as I said. This makes sense, as ACDEF and acdef will be mirror images of each other. I think I was getting mixed up with a paper in Nature Structural Biology on IgE receptor antagonists, where they seemed to be saying that cyclo-(ACEDF) has the same structure as cyclo-(fedca) which looks reasonable to me, but this is another story... Sorry about the confusion caused. Andrew ================================================================== Andrew Wallace, Ph.D., Queen's University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://www.qub.ac.uk/b&bchem/rbiochem.htm From owner-repertoires@net.bio.net Tue Jun 25 23:00:00 1996 Path: biosci!daresbury!yama.mcc.ac.uk!zippy.dct.ac.uk!str-ccsun!strath-cs!queens-belfast.ac.uk!queens-belfast.ac.uk!nntp Newsgroups: bionet.molecules.repertoires Subject: Re: Methods in Enzymology - Volume 267 Message-ID: <1996Jun25.123115.6719@queens-belfast.ac.uk> From: Andrew Wallace Date: 25 Jun 96 12:31:14 GMT References: <4qdr1a$efv@mserv1.dl.ac.uk> Organization: QUB Nntp-Posting-Host: awall.bc.qub.ac.uk X-Mailer: Mozilla 1.1N (Macintosh; I; PPC) MIME-Version: 1.0 X-URL: news:4qdr1a$efv@mserv1.dl.ac.uk Content-Transfer-Encoding: 7bit Content-Type: text/plain; charset=us-ascii Lines: 13 For those in the UK, you can contact Academic Press at Harcourt, Brace and Jovanovich, the publishers on 0171-267-4466 The ISBN for Methods in Enzymology - Volume 267 is 0121-821-684 |======================================================================| | Andrew Wallace,Ph.D., Queen's University Belfast, N. Ireland (UK) | | a.wallace@qub.ac.uk http://www.qub.ac.uk/b&bchem/rbiochem.htm | |======================================================================| From owner-repertoires@net.bio.net Thu Jun 27 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Molreps FAQ for July 1996 Date: 28 Jun 1996 19:00:21 +0100 Lines: 224 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4r16jl$qc9@mserv1.dl.ac.uk> MIME-Version: 1.0 X-Authentication: none Original-To: molreps@dl.ac.uk M O L R E P S F R E Q U E N T L Y A S K E D Q U E S T I O N S ************* ******************* ********* ***************** **1 Where can I obtain libraries?** 1.1 _Phage Libraries_ You can try approaching the following people and organisations: 1.1.1 pIII/6-mer, pIII/15-mer, pVIII-4/15-mer libraries: George Smith, University of Missouri, FAX +1-573-882-0123 1.1.2 pVIII/9-mer, pVIII/9-merCys and pVIII/zinc-finger phagemid libraries: Alessandra Luzzago, IRBM P. Angeletti, luzzago@irbm.it Franco Felici, IRBM P. Angeletti, felici@irbm.it (NON-COMMERCIAL USERS ONLY) 1.1.3 Antibody scFvs with synthetic CDRs: Greg Winter, MRC-LMB Cambridge, gw@mrc-lmb.cam.ac.uk (NON-COMMERCIAL USERS ONLY) 1.1.4 Commercial Sources: Stratagene (Fab fragments in phage lambda) Affymax Pharmacia Cambridge Antibody Technology New England Biolabs (pIII/7-mer). (NEB catalog, p.166). see also this URL -> http://vent.neb.com/neb/phd/phd.html 1.2 _Synthetic Peptide Libraries_ 1.2.1 Commercial Sources: Affymax Selectide Chiron Corporation Most of the major peptide companies will custom-synthesise a library to your requirements. 1.3 _Nucleic Acid Libraries_ (Aptamers) 1.3.1 Jack Szostak at Harvard medical school? 1.3.2 NEXUS corporation (Larry Gold)? 1.4 _Organic Chemical Libraries_ 1.4.1 Affymax? 1.4.2 Parke-Davis (DIVERSOMERS)? **2) Where can I get anti-phage antibodies?** 2.1 anti-pIII MAb from Michael Tesar mte@venus.gbf-braunschweig.d400.de 2.2 anti-pIII polyclonals from GATC, a German company, FAX +49-7531-57313 TEL +49-7531-57204 2.3 rabbit anti-M13 from Stratagene. **3) How can I make my own libraries?** 3.1 _Phage Libraries_ You can obtain suitable vectors and strains from most of the sources in 1). For phagemid work you can get XL-1 Blue cells from Stratagene and M13K07 helper phage from Pharmacia. 3.2 _Synthetic Peptide Libraries_ 3.2.1 Manual synthesis Houghten's "Tea Bag" method Geysen's "Pin" method Both of these can be adapted to produce either support-bound or soluble libraries. 3.2.2 Automated synthesis Chiron Corporation (Zuckermann) Advanced Chemtech (Commercial robot for 75K sterling) 3.3 _Nucleic Acid Libraries_ 3.3.1 PCR methods 3.4 _Organic Chemical Libraries_ 3.4.1 Manual synthesis 3.4.2 Automated synthesis (Advanced Chemtech) **4) How can I analyse the results of my selection?** 4.1 Insert sequencing (phage libraries) 4.2 Micropanning (Smith and Scott, Methods Enzymol. (1993) 217:228-257) 4.3 Dot-blotting (Felici et al., J. Mol. Biol. (1991) 222:301-310) 4.4 ELISA (Smith and Scott, Methods Enzymol. (1993) 217:228-257) (Dente et al. Gene (1994) 148:7-13) 4.5 Colony immunoscreening (Christian et al. J. Mol. Biol. (1992) 227, 711-718) (Felici et al. Gene (1993) 128, 21-27) 4.5 Plaque immunoscreening (Luzzago et al., Gene (1993) 128:51-57) (Felici et al., Methods Enzymol. (1996) 267:116-129) **5) Are there any World Wide Web (WWW) sites about molreps? 5.1 http://www.bio.net/ - The BIOSCI web site itself (MOLREPS message archive) 5.2 http://bionmr1.rug.ac.be/chemistry/overview.html - A useful chemistry site 5.3 http://vesta.pd.com/ - Site for the journal MOLECULAR DIVERSITY 5.4 http://www.mrc-cpe.cam.ac.uk/imt-doc/vbase-home-page.html - Immunoglobulin v-gene database 5.5 http://molbio.info.nih.gov/molbio/desk.html - Molecular biologists desk reference 5.6 http://www.Kairos-scientific.com/ - Kairos scientific home page 5.7 http://www-lmmb.ncifcrf.gov/~toms/ - Tom Schneider's Theory of Molecular Machines 5.8 http://www.ebi.ac.uk/ - The European Bioinformatics Institute (EBI) 5.9 http://www.ebi.ac.uk/primers_home.html - PCR primers database at EBI 5.10 http://aminoacid.bri.nrc.ca:1125/ - Database of building blocks for library synthesis 5.11 http://vent.neb.com/neb/phd/phd.html - PHD commercial phage library at New England Biolabs 5.12 ftp://ftp.netcom.com/pub/qu/quincicc/maxim.html - Another commercial library source ------------------------------------------------------------------ Andrew Wallace, Ph.D, Queen's University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://www.qub.ac.uk/b&bchem/rbiochem.htm From owner-repertoires@net.bio.net Thu Jun 27 23:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molecules.repertoires Subject: IMPORTANT - BIOSCI Fundraising Update! Date: 28 Jun 1996 02:01:02 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 154 Sender: daemon@net.bio.net Distribution: world Message-ID: <199606280900.CAA21861@net.bio.net> NNTP-Posting-Host: net.bio.net BIOSCI is about halfway to its funding goal!! I'm interrupting the usual monthly posting of the BIOSCI miniFAQ to bring you up to date on BIOSCI fundraising progress, a topic of concern to your future use of this resource. Thank you in advance for taking the time to read this message carefully. Last year we announced that BIOSCI was going to adopt the U.S. Public Broadcasting System model to fund its operations after our DOE/NSF grant runs out later this year. Unlike PBS, we are not soliciting contributions from users; we are only selling ads on our Web pages solely to cover our operating costs. Our goal is to seek sponsorships until we build up an operating reserve of about $100,000 and then cease further promotions until we need to build the reserve back up. (The accountants among our readership will be familiar with the problem of deferred revenue which we can not safely utilize until ads have been displayed for a period of time.) We are only about halfway to our funding goal and need to raise further funds to avoid having to curtail services at net.bio.net. Fundraising is time-consuming, however, and we need your help as explained further below. Our operating costs consist of our network connection, phone lines, hardware maintenance (we will be getting newer and faster hardware soon!), plus 0.7 FTE of salaries covering UNIX systems admin, technical support, quality assurance, i.e., testing, of our system, and administrative costs (such as the time it takes to actually find/write/call potential sponsors and raise money!). Although the BIOSCI staff does get compensated for a portion of the work that they do, this project has always received a lot of free after-hours and "vacation" time labor, so we hope that no one will begrudge the time that we do charge to the project to serve you. All of the three part-time staff members, Dave Mack, Julie Lawrence, and myself, have full time day jobs and families in addition to working hard to keep this service running for all of you. Julie and Dave Mack are subcontractors for BIOSCI; my time that is charged to the project defrays a portion of my regular salary instead of adding to my income. Besides having to relocate the project, we were very busy this last year building new infrastructure such as our WWW hypermail interface to the system. This was released last December along with scores of WAIS indices for the newsgroups. Virtually everything is complete, although we do continue to find and fix bugs (many through your helpful feedback!). We are still having some problems with our WAIS indexing. The archives continue to grow rapidly. We are running over 100 indexes now versus three previously and any systems crashes cause greater havoc with the indexing than before! We are still working to fix this as fast as our resources permit and appreciate your patience, but we have been able to automate a lot of the infrastructure to reduce labor as compared to past requirements. We have also implemented new software to make moderation of BIOSCI/bionet newsgroups much easier and combat the growing problem of Internet junk mail and USENET "spamming." About 20% of our groups are now moderated, many of them by the BIOSCI staff! This, for example, made a major difference last year in the quality of content in our EMPLOYMENT/bionet.jobs.offered newsgroup which many commercial concerns and recruiting firms are using **without charge** to recruit candidates for positions in the biological sciences. We are also now in a position to have sponsors for individual newsgroups as you will have noticed if you have visited http://www.bio.net/ and clicked on "Access the BIOSCI/bionet newsgroups" recently. So, how can you help?? ---------------------- As noted above it can take a lot of time to contact potential sponsors if I have to do it all myself. Our request is quite simple. You can do two important things which will take very little time for you individually. First, please use our WWW system at http://www.bio.net/ to access the archives. You can now post or reply to messages via your Web browser. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. Our hope is to quickly raise several large corporate/institutional sponsors on our heavily-used WWW locations (some stats appended below), and then end this sponsorship campaign so that our resources can continue to be used for service provision, not fundraising. Many of our specialty newsgroup WWW archives are still used by small communities of scientists (and they haven't been heavily promoted yet). While these may be valuable niche markets to some advertisers, it will generate more labor and overhead having to find these sponsors, fairly price the locations, and deal with lots of smaller sponsorships than fewer mid-to large sponsors. We are striving to keep our operation as lean and efficient as possible since we are not trying to make careers out of running BIOSCI. We are trying if at all possible to avoid the administrative overhead entailed with processing lots of small payments to reach our fundraising goals. I'd like to thank all of you for your help in advance. In helping us, you are also helping yourselves, not only in keeping this resource available for all of the both large and small research communities that we serve, but also by alleviating the need for us to go back and compete with researchers for tight grant dollars! We promised NSF when we were awarded the BIOSCI grant that we would carry out this mission to make the service self-supporting. With your help, we will succeed in continuing BIOSCI's work into its second decade. Thank you very much! Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net A list of our prime WWW sponsorship locations follow. Please contact us for further details. ---------------------------------------------------------------------- The overall BIOSCI WWW pages are currently visited by users from close to 5500 unique computer hosts per week. Web servers only log the Internet computer/host name and frequently more than one individual can connect to us from a particular host. Main home page, http://www.bio.net, visited recently by about 2100 unique hosts per week Main Newsgroups archives page, http://www.bio.net/archives.html, visited recently by about 1200 Unique hosts per week BIO-JOURNALS archive page, http://www.bio.net/BIO-JOURNALS.html, visited recently by about 1000 unique hosts per week. EMPLOYMENT archive pages: http://www.bio.net:80/hypermail/EMPLOYMENT/ and monthly header pages, visited recently by about 800 unique hosts per week. Address database search page, http://www.bio.net/addrsearch.html, visited recently by about 450 unique hosts per week. Methods newsgroup archive pages, http://www.bio.net:80/hypermail/METHDS- REAGNTS/ and monthly header pages, visited recently by about 350 unique hosts per week. Ads can also be displayed on various combinations of other BIOSCI/bionet newsgroups. Please contact us at biosci-help@net.bio.net for details. ---------------------------------------------------------------------- From owner-repertoires@net.bio.net Sun Jun 30 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Molreps FAQ for July 1996 Date: 1 Jul 1996 11:04:50 +0100 Lines: 229 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4r87s2$7lo@mserv1.dl.ac.uk> MIME-Version: 1.0 X-Authentication: none Original-To: molreps@dl.ac.uk M O L R E P S F R E Q U E N T L Y A S K E D Q U E S T I O N S ************* ******************* ********* ***************** **1 Where can I obtain libraries?** 1.1 _Phage Libraries_ You can try approaching the following people and organisations: 1.1.1 pIII/6-mer, pIII/15-mer, pVIII-4/15-mer libraries: George Smith, University of Missouri, FAX +1-573-882-0123 1.1.2 pVIII/9-mer, pVIII/9-merCys and pVIII/zinc-finger phagemid libraries: Alessandra Luzzago, IRBM P. Angeletti, luzzago@irbm.it Franco Felici, IRBM P. Angeletti, felici@irbm.it (NON-COMMERCIAL USERS ONLY) 1.1.3 Antibody scFvs with synthetic CDRs: Greg Winter, MRC-LMB Cambridge, gw@mrc-lmb.cam.ac.uk (NON-COMMERCIAL USERS ONLY) 1.1.4 Commercial Sources: Stratagene (Fab fragments in phage lambda) Affymax Pharmacia Cambridge Antibody Technology New England Biolabs (pIII/7-mer). (NEB catalog, p.166). see also this URL -> http://vent.neb.com/neb/phd/phd.html 1.2 _Synthetic Peptide Libraries_ 1.2.1 Commercial Sources: Affymax Selectide Chiron Corporation Most of the major peptide companies will custom-synthesise a library to your requirements. 1.3 _Nucleic Acid Libraries_ (Aptamers) 1.3.1 Jack Szostak at Harvard medical school? 1.3.2 NEXUS corporation (Larry Gold)? 1.4 _Organic Chemical Libraries_ 1.4.1 Affymax? 1.4.2 Parke-Davis (DIVERSOMERS)? **2) Where can I get anti-phage antibodies?** 2.1 anti-pIII MAb from Michael Tesar mte@venus.gbf-braunschweig.d400.de 2.2 anti-pIII polyclonals from GATC, a German company, FAX +49-7531-57313 TEL +49-7531-57204 2.3 rabbit anti-M13 from Stratagene. **3) How can I make my own libraries?** 3.1 _Phage Libraries_ You can obtain suitable vectors and strains from most of the sources in 1). For phagemid work you can get XL-1 Blue cells from Stratagene and M13K07 helper phage from Pharmacia. 3.2 _Synthetic Peptide Libraries_ 3.2.1 Manual synthesis Houghten's "Tea Bag" method Geysen's "Pin" method Both of these can be adapted to produce either support-bound or soluble libraries. 3.2.2 Automated synthesis Chiron Corporation (Zuckermann) Advanced Chemtech (Commercial robot for 75K sterling) 3.3 _Nucleic Acid Libraries_ 3.3.1 PCR methods 3.4 _Organic Chemical Libraries_ 3.4.1 Manual synthesis 3.4.2 Automated synthesis (Advanced Chemtech) **4) How can I analyse the results of my selection?** 4.1 Insert sequencing (phage libraries) 4.2 Micropanning (Smith and Scott, Methods Enzymol. (1993) 217:228-257) 4.3 Dot-blotting (Felici et al., J. Mol. Biol. (1991) 222:301-310) 4.4 ELISA (Smith and Scott, Methods Enzymol. (1993) 217:228-257) (Dente et al. Gene (1994) 148:7-13) 4.5 Colony immunoscreening (Christian et al. J. Mol. Biol. (1992) 227, 711-718) (Felici et al. Gene (1993) 128, 21-27) 4.5 Plaque immunoscreening (Luzzago et al., Gene (1993) 128:51-57) (Felici et al., Methods Enzymol. (1996) 267:116-129) **5) Are there any World Wide Web (WWW) sites about molreps?** 5.1 http://www.bio.net/ - The BIOSCI web site itself (MOLREPS message archive) 5.2 http://bionmr1.rug.ac.be/chemistry/overview.html - A useful chemistry site 5.3 http://vesta.pd.com/ - Site for the journal MOLECULAR DIVERSITY 5.4 http://www.mrc-cpe.cam.ac.uk/imt-doc/vbase-home-page.html - Immunoglobulin v-gene database 5.5 http://molbio.info.nih.gov/molbio/desk.html - Molecular biologists desk reference 5.6 http://www.Kairos-scientific.com/ - Kairos scientific home page 5.7 http://www-lmmb.ncifcrf.gov/~toms/ - Tom Schneider's Theory of Molecular Machines 5.8 http://www.ebi.ac.uk/ - The European Bioinformatics Institute (EBI) 5.9 http://www.ebi.ac.uk/primers_home.html - PCR primers database at EBI 5.10 http://aminoacid.bri.nrc.ca:1125/ - Database of building blocks for library synthesis 5.11 http://vent.neb.com/neb/phd/phd.html - PHD commercial phage library at New England Biolabs > 5.12 ftp://ftp.netcom.com/pub/qu/quincicc/maxim.html > - Another commercial library source > This link seems to be broken. As usual, all comments and suggestions or corrections are welcome. Andrew ------------------------------------------------------------------ Andrew Wallace, Ph.D, Queen's University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://www.qub.ac.uk/b&bchem/rbiochem.htm