From owner-repertoires@net.bio.net Mon Jul 01 23:00:00 1996 Path: biosci!PLANTPATH.WISC.EDU!MRS From: MRS@PLANTPATH.WISC.EDU (MIchael R. Sussman) Newsgroups: bionet.molecules.repertoires Subject: Just a wee search for you before I disappear for a week or so. Date: 2 Jul 1996 10:14:02 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 27 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net ********************************************************* SUBJECT: Combinatorial & Phage Libraries DATE: June 24, 1996 ******************************************************** Structural Chemistry 7: 3 (JUN 1996) SJ Cyvin, EK Lloyd, BN Cyvin, J Brunvoll Chemical relevance of a pure combinatorial problem: Isomers of conjugated polyenes 183-186 JAMA - Journal of the American Medical Association 275: 21 (JUN 5 1996) DF Phillips Making new drugs via combinatorial chemistry 1624-1626 International Immunology 8: 5 (MAY 1996) S Matsushita, T Nishi, M Oiso, K Yamaoka, K Yone, T Kanai, Y Nishimura HLA-DQ-binding peptide motifs .1. Comparative binding analysis of type II collagen-derived peptides to DR and DQ molecules of rheumatoid arthritis- susceptible and non-susceptible haplotypes 757-764 From owner-repertoires@net.bio.net Mon Jul 01 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: "Janet Clench, Library, Tel:(39 6)91093220" Newsgroups: bionet.molecules.repertoires Subject: Just a wee search for you before I disappear for a week or so. Date: 2 Jul 1996 14:11:50 +0100 Lines: 30 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4rb76m$q8@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk ********************************************************* SUBJECT: Combinatorial & Phage Libraries DATE: June 24, 1996 ******************************************************** Structural Chemistry 7: 3 (JUN 1996) SJ Cyvin, EK Lloyd, BN Cyvin, J Brunvoll Chemical relevance of a pure combinatorial problem: Isomers of conjugated polyenes 183-186 JAMA - Journal of the American Medical Association 275: 21 (JUN 5 1996) DF Phillips Making new drugs via combinatorial chemistry 1624-1626 International Immunology 8: 5 (MAY 1996) S Matsushita, T Nishi, M Oiso, K Yamaoka, K Yone, T Kanai, Y Nishimura HLA-DQ-binding peptide motifs .1. Comparative binding analysis of type II collagen-derived peptides to DR and DQ molecules of rheumatoid arthritis- susceptible and non-susceptible haplotypes 757-764 From owner-repertoires@net.bio.net Mon Jul 01 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Australian Medicinal Chemistry Conference Date: 2 Jul 1996 11:44:32 +0100 Lines: 111 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4rauig$j8a@mserv1.dl.ac.uk> MIME-Version: 1.0 X-Authentication: none Original-To: molreps@dl.ac.uk On Tue, 2 Jul 1996 12:53:05 +1000 Tracy Nero wrote: >Please excuse multiple copies due to cross posting. > > ROYAL AUSTRALIAN CHEMICAL INSTITUTE > > MEDICINAL & AGRICULTURAL DIVISION 13TH NATIONAL CONFERENCE > > "UP AND COMING RESEARCH IN AUSTRALIA" > > 8-11 DECEMBER, 1996, > Monash University, Clayton 3168, Victoria, Australia > >The conference will cover all aspects of medicinal and agricultural >chemistry with a focus on: > >o students and new researchers >o emerging research areas in medicinal and agricultural chemistry >o the interaction between medicinal chemists, pharmacologists and industry. > >Papers are invited for both lecture and poster sessions. The meeting will >be held in conjunction with the ASCEPT (Australian Society of Clinical and >Experimental Pharmacologists and Toxicologists) and APPS (Australian >Physiological and Pharmacological Society) conferences. > >There will be a joint social mixer and workshop on the initiation of >research/industry interactions on sunday evening. Monday morning >will feature a joint session on mass screening and combinatorial chemistry, >followed in the evening by a joint poster session and wine tasting. > >Invited Speakers Include: Preliminary Session Topics Include: >o Dr George Fleet - glycochemistry, o agrochemistry > herbicides,tuberculosis o glycobiology and glycochemistry > (Oxford University, UK) o toxins >o Dr David Manallack - metalloproteins, o anti-infectives > neural networks (Chiroscience, UK) o enzyme inhibitors >o Dr Lia Addadi - biomineralization o QSAR methods > (Weizman Institute, Israel) o automated methods of >o Dr Shiela Unkles - fungal enzymes synthesis and screening > (Monash University, Aus) o DNA and drugs >o Dr Ron Quinn - mass screening o medicinal chemistry teaching > (Griffith University, Aus) >o Dr John Bremner - medicinal chemistry > teaching (Uni. of Wollongong, Aus) Social Events: >o Dr Mohan Venkataraman - DNA drugs o mixer sunday evening > (ISIS Pharmaceuticals, USA) o wine tasting and mixer after >o Dr Paul Rowland - drug poster session monday evening > development (Medeval Ltd., UK) o conference dinner at Kenloch >o Dr Damon Ridley - chemical restaurant in the Dandenong Ranges > databases (Uni. of Sydney, Aus) (dinner cost for accompanying person $60) >o Prof Colin Masters - prions, > Alzheimers disease > (Uni. of Melbourne, Aus) >o Prof William Denny - cancer research > (Auckland University, NZ) > >Student Scholarships: 10 scholarships of $250 will be available to interstate > students presenting either a talk or poster > >Deadlines: Abstract 30th Sept., 1996 Registration 8th Nov., 1996 > >Registration Costs: > On or before 8th Nov After 8th Nov >RACI Member $300 $400 >non-RACI Member $375 $475 >Reciprocal Society >Member $300 $400 >RACI Student $100 $200 >non-RACI Student $140 $240 > >NOTE 1: Above costs are all in AUSTRALIAN DOLLARS >NOTE 2: All social mixers,the conference dinner, workshop, morning & afternoon > teas and boxed lunches are included in the registration cost > >To receive the conference circular and registration form contact: >o the conference organiser Dr Mick Gould, Conference Associates Pty Ltd, > 13 Jeffrey St, Mt Waverly, Victoria, Australia 3149. > Ph: (03) 9887-8003 Fax: (03) 9887-8773 > email: ca@ozemail.com.au > >o Dr. Margaret Wong, Department of Applied Chemistry,Swinburne University of > Technology, Hawthorn,Victoria, Australia 3122. > Ph: (03) 9214-8542 Fax: (03) 9819-0834 > email: marg@chem1.chem.swin.edu.au > >o Web page http://www.chem.swin.edu.au/ma/mconf.html > (registration forms will be available from the web site at a later date) > > >************************************************************************** >cheers, > >Tracy Nero, PhD >Clinical Pharmacology and Therapeutics Unit, >Dept. of Medicine, The University of Melbourne, >Austin Campus, Austin and Repatriation Medical Centre, >Heidelberg, 3084, Victoria, Australia > >tracy@austin.unimelb.edu.au >Ph. 61 3 94965052 >Fax 61 3 94593510 ------------------------------------------------------------------ Andrew Wallace, Ph.D, Queen's University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://www.qub.ac.uk/b&bchem/rbiochem..htm From owner-repertoires@net.bio.net Tue Jul 02 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Australian Medicinal Chemistry Conference Date: 3 Jul 1996 10:03:28 +0100 Lines: 112 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4rdd10$16e@mserv1.dl.ac.uk> MIME-Version: 1.0 X-Authentication: none Original-To: molreps@dl.ac.uk On Wed, 3 Jul 1996 12:23:50 +1000 Tracy Nero wrote: >Sorry for any inconvenience but the email address for the conference >organiser was incorrect on the earlier posting > >Please excuse multiple copies due to cross posting. > > ROYAL AUSTRALIAN CHEMICAL INSTITUTE > > MEDICINAL & AGRICULTURAL DIVISION 13TH NATIONAL CONFERENCE > > "UP AND COMING RESEARCH IN AUSTRALIA" > > 8-11 DECEMBER, 1996, > Monash University, Clayton 3168, Victoria, Australia > >The conference will cover all aspects of medicinal and agricultural >chemistry with a focus on: > >o students and new researchers >o emerging research areas in medicinal and agricultural chemistry >o the interaction between medicinal chemists, pharmacologists and industry. > >Papers are invited for both lecture and poster sessions. The meeting will >be held in conjunction with the ASCEPT (Australian Society of Clinical and >Experimental Pharmacologists and Toxicologists) and APPS (Australian >Physiological and Pharmacological Society) conferences. > >There will be a joint social mixer and workshop on the initiation of >research/industry interactions on sunday evening. Monday morning >will feature a joint session on mass screening and combinatorial chemistry, >followed in the evening by a joint poster session and wine tasting. > >Invited Speakers Include: Preliminary Session Topics Include: >o Dr George Fleet - glycochemistry, o agrochemistry > herbicides,tuberculosis o glycobiology and glycochemistry > (Oxford University, UK) o toxins >o Dr David Manallack - metalloproteins, o anti-infectives > neural networks (Chiroscience, UK) o enzyme inhibitors >o Dr Lia Addadi - biomineralization o QSAR methods > (Weizman Institute, Israel) o automated methods of >o Dr Shiela Unkles - fungal enzymes synthesis and screening > (Monash University, Aus) o DNA and drugs >o Dr Ron Quinn - mass screening o medicinal chemistry teaching > (Griffith University, Aus) >o Dr John Bremner - medicinal chemistry > teaching (Uni. of Wollongong, Aus) Social Events: >o Dr Mohan Venkataraman - DNA drugs o mixer sunday evening > (ISIS Pharmaceuticals, USA) o wine tasting and mixer after >o Dr Paul Rowland - drug poster session monday evening > development (Medeval Ltd., UK) o conference dinner at Kenloch >o Dr Damon Ridley - chemical restaurant in the Dandenong Ranges > databases (Uni. of Sydney, Aus) (dinner cost for accompanying person $60) >o Prof Colin Masters - prions, > Alzheimers disease > (Uni. of Melbourne, Aus) >o Prof William Denny - cancer research > (Auckland University, NZ) > >Student Scholarships: 10 scholarships of $250 will be available to interstate > students presenting either a talk or poster > >Deadlines: Abstract 30th Sept., 1996 Registration 8th Nov., 1996 > >Registration Costs: > On or before 8th Nov After 8th Nov >RACI Member $300 $400 >non-RACI Member $375 $475 >Reciprocal Society >Member $300 $400 >RACI Student $100 $200 >non-RACI Student $140 $240 > >NOTE 1: Above costs are all in AUSTRALIAN DOLLARS >NOTE 2: All social mixers,the conference dinner, workshop, morning & afternoon > teas and boxed lunches are included in the registration cost > >To receive the conference circular and registration form contact: >o the conference organiser Dr Mick Gould, Conference Associates Pty Ltd, > 13 Jeffrey St, Mt Waverly, Victoria, Australia 3149. > Ph: (03) 9887-8003 Fax: (03) 9887-8773 > email: ca@netwide.com.au > >o Dr. Margaret Wong, Department of Applied Chemistry,Swinburne University of > Technology, Hawthorn,Victoria, Australia 3122. > Ph: (03) 9214-8542 Fax: (03) 9819-0834 > email: marg@chem1.chem.swin.edu.au > >o Web page http://www.chem.swin.edu.au/ma/mconf.html > (registration forms will be available from the web site at a later date) > > >*************************************************************************** >Tracy Nero, PhD >Clinical Pharmacology and Therapeutics Unit, >Dept. of Medicine, The University of Melbourne, >Austin Campus, Austin and Repatriation Medical Centre, >Heidelberg, 3084, Victoria, Australia > >tracy@austin.unimelb.edu.au >Ph. 61 3 94965052 >Fax 61 3 94593510 ------------------------------------------------------------------ Andrew Wallace, Ph.D, Queen's University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://www.qub.ac.uk/b&bchem/rbiochem.htm From owner-repertoires@net.bio.net Sun Jul 07 23:00:00 1996 Path: biosci!ihnp4.ucsd.edu!swrinde!newsfeed.internetmci.com!solaris.cc.vt.edu!usenet From: dgaines@vt.edu (David N. Gaines) Newsgroups: bionet.molbio.yeast,bionet.molec-model,bionet.molecules.peptides,bionet.molecules.repertoires,bionet.mycology,bionet.neuroscience,bionet.neuroscience.amyloid Subject: Re: money making/saving reports Date: 8 Jul 1996 15:18:23 GMT Organization: Virginia Tech/Blacksburg Electronic Village Lines: 9 Message-ID: <4rr8rv$aek@solaris.cc.vt.edu> References: <01bb6c75.8e548da0$608545c7@system> NNTP-Posting-Host: as2511-37.sl002.cns.vt.edu Mime-Version: 1.0 X-Newsreader: WinVN 0.93.14 Xref: biosci bionet.molbio.yeast:5503 bionet.molec-model:1024 bionet.molecules.peptides:358 bionet.molecules.repertoires:187 bionet.mycology:4326 bionet.neuroscience:14800 bionet.neuroscience.amyloid:504 In article <01bb6c75.8e548da0$608545c7@system>, Trythis@Money.com says... > >Get lots of expensive stuff free by paying me What sort of sleasy scam is this, and what does it have to do with mycology??????? From owner-repertoires@net.bio.net Sun Jul 07 23:00:00 1996 Path: biosci!ihnp4.ucsd.edu!swrinde!cs.utexas.edu!howland.reston.ans.net!world1.bawave.com!news2.cais.net!news.cais.net!hunter.premier.net!netnews.worldnet.att.net!newsadm From: "Try this" Newsgroups: bionet.molbio.yeast,bionet.molec-model,bionet.molecules.peptides,bionet.molecules.repertoires,bionet.mycology,bionet.neuroscience,bionet.neuroscience.amyloid Subject: money making/saving reports Date: Sun, 7 Jul 1996 21:31:23 -0500 Organization: AT&T WorldNet Services Lines: 93 Message-ID: <01bb6c75.8e548da0$608545c7@system> NNTP-Posting-Host: 96.dallas-2.tx.dial-access.att.net Mime-Version: 1.0 Content-Type: multipart/mixed; boundary="----=_NextPart_000_01BB6C4B.A57E85A0" Content-Transfer-Encoding: 7bit X-Newsreader: Microsoft Internet News 4.70.1085 Xref: biosci bionet.molbio.yeast:5499 bionet.molec-model:1022 bionet.molecules.peptides:357 bionet.molecules.repertoires:186 bionet.mycology:4323 bionet.neuroscience:14794 bionet.neuroscience.amyloid:503 This is a multi-part message in MIME format. ------=_NextPart_000_01BB6C4B.A57E85A0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 7bit ------=_NextPart_000_01BB6C4B.A57E85A0 Content-Type: text/plain Content-Transfer-Encoding: quoted-printable Content-Description: .txt (Text Document) Content-Disposition: attachment; filename="Money reports.txt" 12 Money making/saving reports worth $125 for $20!!! #1> How to get free subscriptions to over 100 magazines This report is incredible, it tells you exactly where to write for FREE = magazines subscriptions. 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Box 850091 Mesquite, Tx 75185-0091 ------=_NextPart_000_01BB6C4B.A57E85A0-- From owner-repertoires@net.bio.net Mon Jul 08 23:00:00 1996 Newsgroups: bionet.molbio.yeast,bionet.molec-model,bionet.molecules.peptides,bionet.molecules.repertoires,bionet.mycology,bionet.neuroscience,bionet.neuroscience.amyloid Path: biosci!ns1.faseb.org!lamarck.sura.net!fconvx.ncifcrf.gov!kaylor!toms From: toms@kaylor.ncifcrf.gov (Tom Schneider) Subject: Re: money making/saving reports Message-ID: Followup-To: bionet.molbio.yeast,bionet.molec-model,bionet.molecules.peptides,bionet.molecules.repertoires,bionet.mycology,bionet.neuroscience,bionet.neuroscience.amyloid Sender: usenet@ncifcrf.gov (C News) Nntp-Posting-Host: kaylor.ncifcrf.gov Organization: Frederick Biomedical Supercomputer Center X-Newsreader: TIN [version 1.2 PL2] References: <01bb6c75.8e548da0$608545c7@system> <4rr8rv$aek@solaris.cc.vt.edu> <31E23D9F.3094@tiac.com> Date: Tue, 9 Jul 1996 17:35:47 GMT Lines: 53 Xref: biosci bionet.molbio.yeast:5508 bionet.molec-model:1028 bionet.molecules.peptides:361 bionet.molecules.repertoires:189 bionet.mycology:4332 bionet.neuroscience:14811 bionet.neuroscience.amyloid:507 Fred K. Lenherr (lenherr@tiac.com) wrote: : The question is, how do we keep ads/pyramid schemes/junk email out of : the unmoderated newsgroups? You can't. However the people who run the news group can send email to the people on the side. In bionet.info-theory the rule (stated in the faq) is that nobody responds to the postings on the group. If you want to object, you do it by email and so save everybody multiple bother. This has been pretty effective for bionet.info-theory. Generally I send a message to the "perp" and to postmaster@ the same address, asking the postmaster to permanently revoke internet access by the "perp". I also ask the postmaster to teach their people not to do this again. I point out that it has wasted lots of medical scientist's time. On rare occasion they will apologize. Usually they don't have the guts and we never hear from them again. For a the second offense (which has not occured yet) harsher measures can be taken. This is the relevant section in the bionet.info-theory faq (ftp://ftp.ncifcrf.gov/pub/delila/bionet.info-theory.faq): *********************************************************** |-| What Can I Do About Inappropriate Postings? The short form of this news group's name, bio-info, can be a little confusing to some people inexperienced in network communications or with little knowledge of the discipline (if there is any :-) of biological information theory. It can and has been mistaken as a news group for general biological information. Our readers should be aware that when such postings come to our attention, we do attempt to inform, privately, the people who make these inappropriate postings of the error of their ways and suggest alternative or more appropriate venues. Subjecting the writers of inappropriate posting to public excoriation is not a good policy because the mistake is usually inadvertent and the follow-up postings add further to the irritation of our regular readers. When others publicly reply to such posts in this news group, although they may think they are being polite to the original poster, they are still annoying our regular readers. We suggest that a better policy for readers who do wish to reply to inappropriate posts is to do so privately or to an appropriate news group. For information about how to deal with intransigent cases, see: http://math-www.uni-paderborn.de/~axel/blacklist.html *********************************************************** Tom Schneider National Cancer Institute Laboratory of Mathematical Biology Frederick, Maryland 21702-1201 toms@ncifcrf.gov http://www-lmmb.ncifcrf.gov/~toms/ From owner-repertoires@net.bio.net Mon Jul 08 23:00:00 1996 Path: biosci!agate!spool.mu.edu!usenet.eel.ufl.edu!newsfeed.internetmci.com!news.sprintlink.net!new-news.sprintlink.net!news-in.tiac.net!news-old.tiac.net!usenet From: "Fred K. Lenherr" Newsgroups: bionet.molbio.yeast,bionet.molec-model,bionet.molecules.peptides,bionet.molecules.repertoires,bionet.mycology,bionet.neuroscience,bionet.neuroscience.amyloid Subject: Re: money making/saving reports Date: Tue, 09 Jul 1996 07:08:15 -0400 Organization: The Internet Access Company Lines: 25 Message-ID: <31E23D9F.3094@tiac.com> References: <01bb6c75.8e548da0$608545c7@system> <4rr8rv$aek@solaris.cc.vt.edu> NNTP-Posting-Host: 206.119.237.39 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.02Gold (Win95; I) Xref: biosci bionet.molbio.yeast:5506 bionet.molec-model:1025 bionet.molecules.peptides:360 bionet.molecules.repertoires:188 bionet.mycology:4329 bionet.neuroscience:14808 bionet.neuroscience.amyloid:506 David N. Gaines wrote: > > In article <01bb6c75.8e548da0$608545c7@system>, Trythis@Money.com says... > > > >Get lots of expensive stuff free by paying me > > What sort of sleasy scam is this, and what does it have to do with > mycology??????? It's the same sleasy scam described in every book ever written about how to make money in mail order. You buy these "reports" and resell them. The question is, how do we keep ads/pyramid schemes/junk email out of the unmoderated newsgroups? I find that Money.com is retrieved by AltaVista: Welcome to Cambridge Interactive, a company that specializes in harnessing Internet tools to build an Extended Enterprise. We create financial research... http://www.money.com/ - size 4K - 21 Apr 96 But DNS doesn't find it. -- Fred From owner-repertoires@net.bio.net Tue Jul 09 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!math.ohio-state.edu!news.physics.uiowa.edu!newsrelay.iastate.edu!news.iastate.edu!baker From: baker@iastate.edu (Wayne R. Baker) Newsgroups: bionet.molbio.yeast,bionet.molec-model,bionet.molecules.peptides,bionet.molecules.repertoires,bionet.mycology,bionet.neuroscience,bionet.neuroscience.amyloid Subject: Re: money making/saving reports Date: 9 Jul 1996 19:10:13 GMT Organization: Biochemistry & Biophysics, Iowa State University Lines: 54 Message-ID: <4ruaql$l6i@news.iastate.edu> References: <01bb6c75.8e548da0$608545c7@system> <4rr8rv$aek@solaris.cc.vt.edu> <31E23D9F.3094@tiac.com> NNTP-Posting-Host: pv0a0b.vincent.iastate.edu Xref: biosci bionet.molbio.yeast:5513 bionet.molec-model:1031 bionet.molecules.peptides:362 bionet.molecules.repertoires:190 bionet.mycology:4338 bionet.neuroscience:14818 bionet.neuroscience.amyloid:509 In bionet.molec-model, Fred K. Lenherr wrote :David N. Gaines wrote: :> In article <01bb6c75.8e548da0$608545c7@system>, Trythis@Money.com says... :> > :> >Get lots of expensive stuff free by paying me :> :> What sort of sleasy scam is this, and what does it have to do with :> mycology??????? : [ ... ] :The question is, how do we keep ads/pyramid schemes/junk email out of :the unmoderated newsgroups? From the biosci.* FAQ: 1) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups) and mailing lists. The same postings are distributed on both media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. :I find that Money.com is retrieved by AltaVista: The From: line carries little meaning now-days. If you look at the path of the post, you'll find that it originated at att.net: Path: news.iastate.edu!newsrelay.iastate.edu!news.physics.uiowa.edu!newsfeed.ksu.ksu.edu!news.mid.net!newsfeeder.gi.net!newsfeed.internetmci.com!hunter.premier.net!netnews.worldnet.att.net!newsadm ^^^^^^^ money.com doesn't look like they run their own news spool. When I see posts like the one that started this, I forward a copy to news at the site of origin. In trn, this is done by s | mail -s "(fwd) money making/saving reports" news@att.net If the newsadmin gets enough complaints, they'll take action. I'm over in bionet.molec-model which has low enough traffic that posts like this are annoying, but not too frequent. If it's worse in your group, you could opt for moderation but that doesn't guarantee stuff like this won't show up again. Getting boneheads like the original poster spanked is about all that can be done. -- Wayne Baker (baker@iastate.edu) He who has a why to live for 4288 Molecular Biology Building can bear almost any how. Iowa State University --Nietzsche Ames IA 50011 (515) 294 0781 From owner-repertoires@net.bio.net Wed Jul 10 23:00:00 1996 Path: biosci!rutgers!uwm.edu!spool.mu.edu!usenet.eel.ufl.edu!news-feed-1.peachnet.edu!news.service.emory.edu!news From: Brett Burkholder Newsgroups: bionet.molbio.yeast,bionet.molec-model,bionet.molecules.peptides,bionet.molecules.repertoires,bionet.mycology,bionet.neuroscience,bionet.neuroscience.amyloid Subject: Re: money making/saving reports Date: Thu, 11 Jul 1996 16:17:18 +0000 Organization: Emory University Lines: 17 Message-ID: <31E5290E.4813@gmm.gen.emory.edu> References: <01bb6c75.8e548da0$608545c7@system> NNTP-Posting-Host: djdgw486.gen.emory.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01 (Win16; I) To: Try this CC: burkhold@gmm.gen.emory.edu, postmaster@gmm.gen..emory.edu Xref: biosci bionet.molbio.yeast:5517 bionet.molec-model:1037 bionet.molecules.peptides:366 bionet.molecules.repertoires:192 bionet.mycology:4352 bionet.neuroscience:14846 bionet.neuroscience.amyloid:517 Your posting to the group bionet.molbio.yeast was inappropriate. This is the equivalent of e-junk-mail, and I, for one, would appreciate your refraining form such posting in the future. I see you have tried to cleverly hide your posting address. Let me assure you that no posting is completely anonymous, and someone CAN find out who you are, if they are so inclined. If you continue to make such inappropriate postings--an act known as "spamming"--we can find out where you are gaining net access and contact the postmaster or SYSOP of that system. Postmasters and SYSOP's, even commercial access companies such as AOL, frown heavily upon spamming. You may even lose your net access if you continue to ignore nettiquette. Send your junk mail somewhere else. Brett Burkholder From owner-repertoires@net.bio.net Tue Jul 16 23:00:00 1996 Path: biosci!news.Stanford.EDU!newshub.internex.net!newshub1.internex.net!news.sprintlink.net!news-stk-11.sprintlink.net!news.sprintlink.net!new-news.sprintlink.net!newsreader.sprintlink.net!news.sprintlink.net!news-dc-10.sprintlink.net!nntp.primenet.com!news.cais.net!hunter.premier.net!netnews.worldnet.att.net!ix.netcom.com!netcom.net.uk!sunsite.doc.ic.ac.uk!nntp0.brunel.ac.uk!strath-cs!queens-belfast.ac.uk!queens-belfast.ac.uk!nntp Newsgroups: bionet.molecules.repertoires Subject: Discussion Message-ID: <31ECA95C.1511@queens-belfast.ac.uk> From: Andrew Wallace Date: Wed, 17 Jul 1996 09:50:36 +0100 Nntp-Posting-Host: awall.bc.qub.ac.uk X-Mailer: Mozilla 2.0 (Macintosh; I; PPC) MIME-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Lines: 14 I see that people are having a nice discussion on the mol-diversity list about automated organic synthesisers. We are thinking of buying the 396 multiple peptide synthesiser from Advanced Chemtech. Does anyone out there have one of these and if so, what do you think of it? Andrew P.S. Sorry about all the spamming, I think the summer heat must be getting to some people... -- ====================================================================== Andrew Wallace,Ph.D., Queen's University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://www.qub.ac.uk/b&bchem/rbiochem.htm ====================================================================== From owner-repertoires@net.bio.net Sun Jul 21 23:00:00 1996 Path: biosci!agate!howland.reston.ans.net!EU.net!Norway.EU.net!nntp.uio.no!news.cais.net!mr.net!news.ios.com!usenet From: shahvika@pilot.msu.edu (Vikas Shah) Newsgroups: bionet.molecules.repertoires Subject: Molecular Modelling Date: Mon, 22 Jul 1996 16:17:00 GMT Organization: Michigan State University Lines: 14 Sender: Vikas Shah Message-ID: <31f3a974.2747691@news.ios.com> Reply-To: shahvika@pilot.msu.edu NNTP-Posting-Host: ppp-21.ts-1.hck.idt.net X-Newsreader: Forte Agent .99e/32.227 I am an undergraduate student at Michigan State University, entering my junior year this fall. I am investigating molecular modelling packages for research I plan on doing next summer, and am writing to ask for help and recommendations regarding the wide variety of packages out there. I need to be able to do energy minimizations as well as geometry optimizations, predict electron densities, zoom in and out among structures, and - if possible - view and record particle-particle interactions. Here's the catch -- I am running an IBM 486DX2 66 Mhz with 20 MB of RAM and 1 MB vRAM with Win95. I am planning on upgrading if possible, but that will depend on funding. Forget system speed for now, I just need to know the best packages out there. Please mail shahvika@pilot.msu.edu. Thank you. Vikas Shah From owner-repertoires@net.bio.net Wed Jul 24 23:00:00 1996 Path: biosci!mjr.com!markn From: markn@mjr.com (Mark Norman) Newsgroups: bionet.molecules.repertoires Subject: (none) Date: 25 Jul 1996 15:32:15 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net unsubscribe markn@mjr.com Mark Norman MJ Research 840 Taraval St., Ste. 100 San Francisco, CA 94116 415-664-2994 -2995 (fax) markn@mjr.com From owner-repertoires@net.bio.net Wed Jul 24 23:00:00 1996 Path: biosci!news.Stanford.EDU!nntp-hub2.barrnet.net!newsfeed.internetmci.com!info.ucla.edu!unixg.ubc.ca!lpss From: lpss@unixg.ubc.ca (Alex Chang) Newsgroups: bionet.molecules.repertoires Subject: Use of triethylamine to elute phage Date: 25 Jul 1996 19:43:25 GMT Organization: University of British Columbia, Vancouver, B.C., Canada Lines: 12 Message-ID: <4t8iot$b1c@nntp.ucs.ubc.ca> NNTP-Posting-Host: netinfo.ubc.ca X-Newsreader: TIN [version 1.2 PL2] I have a protocol from a paper for immuno-panning using Nunc's immunotube. For the phage elution, triethylamine is used to incubate for 10 min, followed by the addition of certain amount of 1 M Tris-HCl, pH 7.4. I am wondering anyone using triethylamine for this purpose, and what is the mechanism of its use. Thanks Alex Chang Pathology University of British Columbia achang@hivnet.ubc.ca From owner-repertoires@net.bio.net Wed Jul 24 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Alexy Eroshkin Newsgroups: bionet.molecules.repertoires Subject: ANNOUNCEMENT: Protein Multiple Sequences Edinor for Win 3.x/95 Date: 25 Jul 1996 11:37:52 +0100 Organization: State Research Center of Virology & Biotechnology VECTOR Lines: 46 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4t7iq0$qt@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk To: molreps@dl.ac.uk From: eroshkin@vector.nsk.su Subject: ANNOUNCEMENT: Protein Multiple Sequences Editor for Win 3.x/95 Dear All, With the increasing of protein sequence data, multiple alignment is one of the important techniques to understand protein structure-function organization and evolution. New software package ProMSED, Protein Multiple Sequences EDitor for Windows 3.x/95, is available from EBI software library: ftp://ftp.ebi.ac.uk/pub/software/dos/promsed/ (as self-extracted archive). ProMSED is an easy-to-use application for Windows 3.x/95 that performs automatic and manual multiple protein sequences alignment, alignment analysis and editing. The program reads NBRF/PIR, Pearson (Fasta), EMBL/SwissProt, Intelligenetics and CLUSTAL data formats and has interface and the main functions similar to popular text editor Word. ProMSED loads up to eight protein families in time, adds sequences to existing alignment, combines sequences from different files, outputs the alignment in two formats, prints the alignment. Automatic alignment is based on ClustalV algorithm. Manual alignment and sequence analysis are facilitated by using group operations and amino acid color-coding reflecting amino acid similarity in physico-chemical and mutational properties, secondary structure propensities, etc. ProMSED can align complete set of sequences, its subset and any selected block, providing thus flexible tool for sequences analysis, visualization, edition and illustrations preparation. A HELP is included. Demo version has limits on length and number of sequences. Special thanks to Dr. Desmond Higgins for source code of ClustalV. Comments, bug reports and suggestions for new features are welcome and should be sent by email to eroshkin@vector.nsk.su. Inquiries can be addressed to: ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Dr. Alexey Eroshkin Institute of Molecular Biology E.mail: eroshkin@vector.nsk.su State Research Center of Virology and Tel: +7 (3832) - 647774 Biotechnology "Vector" Fax: +7 (3832) - 328831 Koltsovo, Novosibirsk Region 633159 Russia ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ From owner-repertoires@net.bio.net Thu Jul 25 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: "Janet Clench, Library, Tel:(39 6)91093220" Newsgroups: bionet.molecules.repertoires Subject: And another one bites the dust ... Date: 26 Jul 1996 14:43:22 +0100 Lines: 283 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4tai1q$6s4@mserv1.dl.ac.uk> Original-To: molreps@dl.ac.uk ******************************************************** SUBJECT: Combinatorial & Phage Libraries DATE: July 1, 8, 15, 22, 1996 ******************************************************** Journal of Inorganic Biochemistry 63: 1 (JUL 1996) T Whang, B Daly, J Yin Metal-ion discrimination by phage T7 1-7 Science 272: 5270 (JUN 28 1996) MK Waldor, JJ Mekalanos Lysogenic conversion by a filamentous phage encoding cholera toxin 1910-1914 Chemical Communications: 11 (JUN 7 1996) TM Krulle, B Davis, H Ardron, DD Long, NA Hindle, C Smith, D Brown, AL Lane, DJ Watkin, DG Marquess, GWJ Fleet Kinetic and thermodynamic azides from alpha-triflates of gamma- lactones: Intermediates for the incorporation of polyhydroxylated D- and L-alpha-aminoacids into combinatorial libraries 1271-1272 Talanta 43: 2 (FEB 1996) TF Kumosinski, JJ Unruh Quantitation of the global secondary structure of globular proteins by FTIR spectroscopy: Comparison with X-ray crystallographic structure 199-219 Tetrahedron Letters 37: 23 (JUN 3 1996) OL Acevedo, RS Andrews Synthesis of propane-2,3-diol combinatorial monomers 3931-3934 Molecular Immunology 33: 3 (FEB 1996) M Duenas, AC Malmborg, R Casalvilla, M Ohlin, CAK Borrebaeck Selection of phage displayed antibodies based on kinetic constants 279-285 Journal of Molecular Biology 259: 3 (JUN 14 1996) J Fan, AD Griffiths, A Lockhart, RA Cross, LA Amos Microtubule minus ends can be labelled with a phage display antibody specific to alpha-tubulin 325-330 Journal of Molecular Biology 259: 3 (JUN 14 1996) SA Overman, M Tsuboi, GJ Thomas Subunit orientation in the filamentous virus Ff(fd, f1, M13) 331-336 Molecular Microbiology 20: 4 (MAY 1996) HR Hill, PG Stockley Phage presentation 685-692 Biotechniques 20: 6 (JUN 1996) B Andersson, J Lu, KE Edwards, DM Muzny, RA Gibbs Method for 96-well M13 DNA template preparations for large-scale sequencing 1022-1027 Biotechniques 20: 6 (JUN 1996) K Jacobsson, L Frykberg Phage display shot-gun cloning of ligand-binding domains of prokaryotic receptors approaches 100% correct clones 1070 Oncogene 12: 11 (JUN 6 1996) DP Lane, CW Stephen, CA Midgley, A Sparks, TR Hupp, DA Daniels, R Greaves, A Reid, B Vojtesek, SM Picksley Epitope analysis of the murine p53 tumour suppressor protein 2461-2466 EMBO Journal 15: 11 (JUN 3 1996) C Toniatti, A Cabibbo, E Sporeno, AL Salvati, M Cerretani, S Serafini, A Lahm, R Cortese, G Ciliberto Engineering human interleukin-6 to obtain variants with strongly enhanced bioactivity 2726-2737 Biopolymers 39: 1 (JUL 1996) JX Tang, S Fraden Nonmonotonic temperature dependence of the flexibility of bacteriophage fd 13-22 Xenotransplantation 3: 1 Part 1 (FEB 1996) HA Vaughan, KR Oldenburg, MA Gallop, JD Atkin, IFC Mckenzie, MS Sandrin Recognition of an octapeptide sequence by multiple Gal alpha(1,3)Gal- binding proteins 18-23 Journal of Virological Methods 58: 1-2 (APR 26 1996) V Germaschewski, K Murray Identification of polyclonal serum specificities with phage-display libraries 21-32 Gene 171: 1 (MAY 24 1996) X Cheng, BK Kay, RL Juliano Identification of a biologically significant DNA-binding peptide motif by use of a random phage display library 1-8 Gene 171: 1 (MAY 24 1996) P Malik, RN Perham New vectors for peptide display on the surface of filamentous bacteriophage 49-51 Gene 171: 1 (MAY 24 1996) PJ Dyson, M Evans pUCS75, a stable high-copy-number Streptomyces Escherichia coli shuttle vector which facilitates subcloning from pUC plasmid and M13 phage vectors 71-73 Journal of Molecular Biology 259: 4 (JUN 21 1996) JD Mcbride, N Freeman, GJ Domingo, RJ Leatherbarrow Selection of chymotrypsin inhibitors from a conformationally- constrained combinatorial peptide library 819-827 Combinatorial Chemistry (Series: Methods in Enzymology 267 (1996)) JN Yu, GP Smith Affinity maturation of phage-displayed peptide ligands 3-27 Combinatorial Chemistry (Series: Methods in Enzymology 267 (1996)) W Markland, BL Roberts, RC Ladner Selection for protease inhibitors using bacteriophage display 28-51 Combinatorial Chemistry (Series: Methods in Enzymology 267 (1996)) CI Wang, Q Yang, CS Craik Phage display of proteases and macromolecular inhibitors 52-68 Combinatorial Chemistry (Series: Methods in Enzymology 267 (1996)) BL Roberts, W Markland, RC Ladner Affinity maturation of proteins displayed on surface of M13 bacteriophage as major coat protein fusions 68-82 Combinatorial Chemistry (Series: Methods in Enzymology 267 (1996)) JL Harrison, SC Williams, G Winter, A Nissim Screening of phage antibody libraries 83-109 Combinatorial Chemistry (Series: Methods in Enzymology 267 (1996)) G Galfre, P Monaci, A Nicosia, A Luzzago, F Felici, R Cortese Immunization with phage-displayed mimotopes 109-115 Combinatorial Chemistry (Series: Methods in Enzymology 267 (1996)) F Felici, G Galfre, A Luzzago, P Monaci, A Nicosia, R Cortese Phage-displayed peptides as tools for characterization of human sera 116-129 Combinatorial Chemistry (Series: Methods in Enzymology 267 (1996)) EJ Rebar, HA Greisman, CO Pabo Phage display methods for selecting zinc finger proteins with novel DNA-binding specificities 129-149 Combinatorial Chemistry (Series: Methods in Enzymology 267 (1996)) IA Lairdoffringa, JG Belasco In vitro genetic analysis of RNA-binding proteins using phage display libraries 149-168 Combinatorial Chemistry (Series: Methods in Enzymology 267 (1996)) PJ Schatz, MG Cull, EL Martin, CM Gates Screening of peptide libraries linked to lac repressor 171-191 Combinatorial Chemistry (Series: Methods in Enzymology 267 (1996)) LC Mattheakis, JM Dias, WJ Dower Cell-free synthesis of peptide libraries displayed on polysomes 195-207 Combinatorial Chemistry (Series: Methods in Enzymology 267 (1996)) CL Chen, P Strop, M Lebl, KS Lam One bead-one compound combinatorial peptide library: Different types of screening 211-219 Combinatorial Chemistry (Series: Methods in Enzymology 267 (1996)) JM Ostresh, SE Blondelle, B Dorner, RA Houghten Generation and use of nonsupport-bound peptide and peptidomimetic combinatorial libraries 220-234 Combinatorial Chemistry (Series: Methods in Enzymology 267 (1996)) KD Janda, HS Han Combinatorial chemistry: A liquid-phase approach 234-247 Combinatorial Chemistry (Series: Methods in Enzymology 267 (1996)) ZJ Ni, D Maclean, CP Holmes, MA Gallop Encoded combinatorial chemistry: Binary coding using chemically robust secondary amine tags 261-272 Combinatorial Chemistry (Series: Methods in Enzymology 267 (1996)) PE Nielsen Peptide-nucleic acids: A new dimension to peptide libraries and aptamers 426-433 Combinatorial Chemistry (Series: Methods in Enzymology 267 (1996)) GM Figliozzi, R Goldsmith, SC Ng, SC Banville, RN Zuckermann Synthesis of N-substituted glycine peptoid libraries 437-447 Combinatorial Chemistry (Series: Methods in Enzymology 267 (1996)) BA Bunin, MJ Plunkett, JA Ellman Synthesis and evaluation of 1,4-benzodiazepine libraries 448-465 Biochemistry 35: 24 (JUN 18 1996) W Markland, AC Ley, SW Lee, RC Ladner Iterative optimization of high-affinity protease inhibitors using phage display .1. Plasmin 8045-8057 Biochemistry 35: 24 (JUN 18 1996) W Markland, AC Ley, RC Ladner Iterative optimization of high-affinity protease inhibitors using phage display .2. Plasma kallikrein and thrombin 8058-8067 European Journal of Biochemistry 238: 3 (JUN 15 1996) BH Rietman, PJM Folkers, RHA Folmer, GI Tesser, CW Hilbers The solution structure of the synthetic circular peptide CGVSRQGKPYC NMR studies of the folding of a synthetic model for the DNA-binding loop of the ssDNA-binding protein encoded by gene V of phage M13 706-713 Journal of Inorganic Biochemistry 63: 1 (JUL 1996) T Whang, B Daly, J Yin Metal-ion discrimination by phage T7 1-7 Journal of Autoimmunity 9: 3 (JUN 1996) C Mennuni, C Santini, F Dotta, L Farilla, U Dimario, A Fierabracci, G Bottazzo, R Cortese, A Luzzago Selection of phage-displayed peptides mimicking type 1 diabetes- specific epitopes 431-436 Molecular Immunology 33: 4-5 (MAR-APR 1996) GR Pilkington, LX Duan, MH Zhu, W Keil, RJ Pomerantz Recombinant human fab antibody fragments to HIV-1 REV and tat regulatory proteins: Direct selection from a combinatorial phage display library 439-450 Antiviral Research 31: 1-2 (JUN 1996) K Mori, T Kubo, Y Kibayashi, T Ohkuma, A Kaji Anti-vaccinia virus effect of M13 bacteriophage DNA 79-86 Science 272: 5270 (JUN 28 1996) MK Waldor, JJ Mekalanos Lysogenic conversion by a filamentous phage encoding cholera toxin 1910-1914 Chemical & Pharmaceutical Bulletin 44: 6 (JUN 1996) K Sagi, H Kagechika, H Fukasawa, Y Hashimoto, K Shudo Deiodotrifluoromethylation of ethyl 3,3',5-triiodothyroacetate. Divergent derivatization based on the combinatorial concept 1273-1275 Expert Opinion on Therapeutic Patents 6: 5 (MAY 1996) AJT George, AA Epenetos Advances in antibody engineering 441-456 From owner-repertoires@net.bio.net Thu Jul 25 23:00:00 1996 Path: biosci!agate!howland.reston.ans.net!EU.net!sun4nl!wirehub!news.euro.net!xs4all!usenet From: tvink@xs4all.nl (Tom Vink) Newsgroups: bionet.molecules.repertoires Subject: Re: Use of triethylamine to elute phage Date: Fri, 26 Jul 1996 08:16:21 GMT Organization: XS4ALL, networking for the masses Lines: 22 Message-ID: <4ta2dd$bu4@news.xs4all.nl> References: <4t8iot$b1c@nntp.ucs.ubc.ca> NNTP-Posting-Host: mas02-13.dial.xs4all.nl X-XS4ALL-Date: Fri, 26 Jul 1996 11:16:29 MET DST X-Newsreader: Forte Free Agent 1.0.82 lpss@unixg.ubc.ca (Alex Chang) wrote: >I have a protocol from a paper for immuno-panning using Nunc's >immunotube. For the phage elution, triethylamine is used to incubate for >10 min, followed by the addition of certain amount of 1 M Tris-HCl, pH >7.4. I am wondering anyone using triethylamine for this purpose, and what >is the mechanism of its use. >Thanks >Alex Chang >Pathology >University of British Columbia >achang@hivnet.ubc.ca Dear Alex, High pH (pH 11), will elute your phage from your ligand. Alternatives are low pH or specific competitors in your system Greetings Tom From owner-repertoires@net.bio.net Sat Jul 27 23:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molecules.repertoires Subject: IMPORTANT - BIOSCI Fundraising Update! Date: 28 Jul 1996 02:00:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 154 Sender: daemon@net.bio.net Distribution: world Message-ID: <199607280900.CAA13626@net.bio.net> NNTP-Posting-Host: net.bio.net BIOSCI is about halfway to its funding goal!! I'm interrupting the usual monthly posting of the BIOSCI miniFAQ to bring you up to date on BIOSCI fundraising progress, a topic of concern to your future use of this resource. Thank you in advance for taking the time to read this message carefully. Last year we announced that BIOSCI was going to adopt the U.S. Public Broadcasting System model to fund its operations after our DOE/NSF grant runs out later this year. Unlike PBS, we are not soliciting contributions from users; we are only selling ads on our Web pages solely to cover our operating costs. Our goal is to seek sponsorships until we build up an operating reserve of about $100,000 and then cease further promotions until we need to build the reserve back up. (The accountants among our readership will be familiar with the problem of deferred revenue which we can not safely utilize until ads have been displayed for a period of time.) We are only about halfway to our funding goal and need to raise further funds to avoid having to curtail services at net.bio.net. Fundraising is time-consuming, however, and we need your help as explained further below. Our operating costs consist of our network connection, phone lines, hardware maintenance (we will be getting newer and faster hardware soon!), plus 0.7 FTE of salaries covering UNIX systems admin, technical support, quality assurance, i.e., testing, of our system, and administrative costs (such as the time it takes to actually find/write/call potential sponsors and raise money!). Although the BIOSCI staff does get compensated for a portion of the work that they do, this project has always received a lot of free after-hours and "vacation" time labor, so we hope that no one will begrudge the time that we do charge to the project to serve you. All of the three part-time staff members, Dave Mack, Julie Lawrence, and myself, have full time day jobs and families in addition to working hard to keep this service running for all of you. Julie and Dave Mack are subcontractors for BIOSCI; my time that is charged to the project defrays a portion of my regular salary instead of adding to my income. Besides having to relocate the project, we were very busy this last year building new infrastructure such as our WWW hypermail interface to the system. This was released last December along with scores of WAIS indices for the newsgroups. Virtually everything is complete, although we do continue to find and fix bugs (many through your helpful feedback!). We are still having some problems with our WAIS indexing. The archives continue to grow rapidly. We are running over 100 indexes now versus three previously and any systems crashes cause greater havoc with the indexing than before! We are still working to fix this as fast as our resources permit and appreciate your patience, but we have been able to automate a lot of the infrastructure to reduce labor as compared to past requirements. We have also implemented new software to make moderation of BIOSCI/bionet newsgroups much easier and combat the growing problem of Internet junk mail and USENET "spamming." About 20% of our groups are now moderated, many of them by the BIOSCI staff! This, for example, made a major difference last year in the quality of content in our EMPLOYMENT/bionet.jobs.offered newsgroup which many commercial concerns and recruiting firms are using **without charge** to recruit candidates for positions in the biological sciences. We are also now in a position to have sponsors for individual newsgroups as you will have noticed if you have visited http://www.bio.net/ and clicked on "Access the BIOSCI/bionet newsgroups" recently. So, how can you help?? ---------------------- As noted above it can take a lot of time to contact potential sponsors if I have to do it all myself. Our request is quite simple. You can do two important things which will take very little time for you individually. First, please use our WWW system at http://www.bio.net/ to access the archives. You can now post or reply to messages via your Web browser. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. Our hope is to quickly raise several large corporate/institutional sponsors on our heavily-used WWW locations (some stats appended below), and then end this sponsorship campaign so that our resources can continue to be used for service provision, not fundraising. Many of our specialty newsgroup WWW archives are still used by small communities of scientists (and they haven't been heavily promoted yet). While these may be valuable niche markets to some advertisers, it will generate more labor and overhead having to find these sponsors, fairly price the locations, and deal with lots of smaller sponsorships than fewer mid-to large sponsors. We are striving to keep our operation as lean and efficient as possible since we are not trying to make careers out of running BIOSCI. We are trying if at all possible to avoid the administrative overhead entailed with processing lots of small payments to reach our fundraising goals. I'd like to thank all of you for your help in advance. In helping us, you are also helping yourselves, not only in keeping this resource available for all of the both large and small research communities that we serve, but also by alleviating the need for us to go back and compete with researchers for tight grant dollars! We promised NSF when we were awarded the BIOSCI grant that we would carry out this mission to make the service self-supporting. With your help, we will succeed in continuing BIOSCI's work into its second decade. Thank you very much! Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net A list of our prime WWW sponsorship locations follow. Please contact us for further details. ---------------------------------------------------------------------- The overall BIOSCI WWW pages are currently visited by users from close to 5500 unique computer hosts per week. Web servers only log the Internet computer/host name and frequently more than one individual can connect to us from a particular host. Main home page, http://www.bio.net, visited recently by about 2100 unique hosts per week Main Newsgroups archives page, http://www.bio.net/archives.html, visited recently by about 1200 Unique hosts per week BIO-JOURNALS archive page, http://www.bio.net/BIO-JOURNALS.html, visited recently by about 1000 unique hosts per week. EMPLOYMENT archive pages: http://www.bio.net:80/hypermail/EMPLOYMENT/ and monthly header pages, visited recently by about 800 unique hosts per week. Address database search page, http://www.bio.net/addrsearch.html, visited recently by about 450 unique hosts per week. Methods newsgroup archive pages, http://www.bio.net:80/hypermail/METHDS- REAGNTS/ and monthly header pages, visited recently by about 350 unique hosts per week. Ads can also be displayed on various combinations of other BIOSCI/bionet newsgroups. Please contact us at biosci-help@net.bio.net for details. ---------------------------------------------------------------------- From owner-repertoires@net.bio.net Mon Jul 29 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Postdoc available for chemist at QUB Date: 30 Jul 1996 17:31:31 +0100 Lines: 31 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4tldd3$s8a@mserv1.dl.ac.uk> MIME-Version: 1.0 The Queen's University of Belfast Postdoctoral Research Fellowship School of Chemistry This post, funded by the Engineering and Physical Sciences Research Council (UK), is available for 1 year from October 1996, to work on an interdisciplinary project involving the Schools of Chemistry and Biology and Biochemistry. The project concerns the use of solid phase synthetic methods to generate libraries of novel chemically modified amino acids and peptides. Applicants must hold an honours degree or equivalent in chemistry and a PhD in organic chemistry (or have submitted a thesis by 1 October 1996). Experience in organic synthesis is essential and experience of amino acid and peptide chemistry is desirable. Salary range: 14,319 - 15,987 pounds sterling per annum, placing depending on age, experience and qualifications. Applicants, quoting Ref. 96/F038, may obtain further particulars from the Personnel Office, The Queen's University of Belfast, BT7 1NN, Northern Ireland (UK), Fax: +44-1232-324944. Closing date: 9th August 1996 -- Note: please do not send email to me as I cannot forward messages on your behalf - apply directly to Personnel. |======================================================================| | Andrew Wallace,Ph.D., Queen's University Belfast, N. Ireland (UK) | | a.wallace@qub.ac.uk http://www.qub.ac.uk/b&bchem/rbiochem.htm | |======================================================================| From owner-repertoires@net.bio.net Mon Jul 29 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Re: Discussion Date: 30 Jul 1996 16:09:45 +0100 Lines: 28 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4tl8jp$n72@mserv1.dl.ac.uk> MIME-Version: 1.0 X-Authentication: none Original-To: molreps@dl.ac.uk On Fri, 19 Jul 96 12:20:39 -0400 Thomas Miller wrote: >Andrew, we have a couple Advanced Chemtech peptide synthesizers (I think >the 357 model) >in our building. Our project actually used one of them for large scale >prep. >When it worked, it was ok. It did require alot of babysitting especially >on runs over >the weekend. Our lab now runs a Perseptive Biosystem 9050 peptide synthesizer. >I sleep a lot better at night. Good luck, Tom. > >ps: Advanced Chemtech support is almost non-existent. My own opinion. > >-- >Opinions are my own and not of my employer. I agree with the reliability of the 9050, the lab at IRBM has one and it is great. By the way, how do you do multiple synthesis on a 9050? I need something that can make 20-40 peptides at once, the 9050 can only do three at a time as far as I know... ------------------------------------------------------------------ Andrew Wallace, Ph.D, Queen's University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://www.qub.ac.uk/b&bchem/rbiochem.htm From owner-repertoires@net.bio.net Wed Jul 31 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Molreps FAQ for August 1996 Date: 1 Aug 1996 12:59:26 +0100 Lines: 226 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4tq66u$k7q@mserv1.dl.ac.uk> MIME-Version: 1.0 X-Authentication: none Original-To: molreps@dl.ac.uk M O L R E P S F R E Q U E N T L Y A S K E D Q U E S T I O N S ************* ******************* ********* ***************** **1 Where can I obtain libraries?** 1.1 _Phage Libraries_ You can try approaching the following people and organisations: 1.1.1 pIII/6-mer, pIII/15-mer, pVIII-4/15-mer libraries: George Smith, University of Missouri, FAX +1-573-882-0123 1.1.2 pVIII/9-mer, pVIII/9-merCys and pVIII/zinc-finger phagemid libraries: Alessandra Luzzago, IRBM P. Angeletti, luzzago@irbm.it Franco Felici, IRBM P. Angeletti, felici@irbm.it (NON-COMMERCIAL USERS ONLY) 1.1.3 Antibody scFvs with synthetic CDRs: Greg Winter, MRC-LMB Cambridge, gw@mrc-lmb.cam.ac.uk (NON-COMMERCIAL USERS ONLY) 1.1.4 Commercial Sources: Stratagene (Fab fragments in phage lambda) Affymax Pharmacia Cambridge Antibody Technology New England Biolabs (pIII/7-mer). (NEB catalog, p.166). see also this URL -> http://vent.neb.com/neb/phd/phd.html 1.2 _Synthetic Peptide Libraries_ 1.2.1 Commercial Sources: Affymax Selectide Chiron Corporation Most of the major peptide companies will custom-synthesise a library to your requirements. 1.3 _Nucleic Acid Libraries_ (Aptamers) 1.3.1 Jack Szostak at Harvard medical school? 1.3.2 NEXUS corporation (Larry Gold)? 1.4 _Organic Chemical Libraries_ 1.4.1 Affymax? 1.4.2 Parke-Davis (DIVERSOMERS)? **2) Where can I get anti-phage antibodies?** 2.1 anti-pIII MAb from Michael Tesar mte@venus.gbf-braunschweig.d400.de 2.2 anti-pIII polyclonals from GATC, a German company, FAX +49-7531-57313 TEL +49-7531-57204 2.3 rabbit anti-M13 from Stratagene. **3) How can I make my own libraries?** 3.1 _Phage Libraries_ You can obtain suitable vectors and strains from most of the sources in 1). For phagemid work you can get XL-1 Blue cells from Stratagene and M13K07 helper phage from Pharmacia. 3.2 _Synthetic Peptide Libraries_ 3.2.1 Manual synthesis Houghten's "Tea Bag" method Geysen's "Pin" method Both of these can be adapted to produce either support-bound or soluble libraries. 3.2.2 Automated synthesis Chiron Corporation (Zuckermann) Advanced Chemtech (Commercial robot for 75K sterling) 3.3 _Nucleic Acid Libraries_ 3.3.1 PCR methods 3.4 _Organic Chemical Libraries_ 3.4.1 Manual synthesis 3.4.2 Automated synthesis (Advanced Chemtech) **4) How can I analyse the results of my selection?** 4.1 Insert sequencing (phage libraries) 4.2 Micropanning (Smith and Scott, Methods Enzymol. (1993) 217:228-257) 4.3 Dot-blotting (Felici et al., J. Mol. Biol. (1991) 222:301-310) 4.4 ELISA (Smith and Scott, Methods Enzymol. (1993) 217:228-257) (Dente et al. Gene (1994) 148:7-13) 4.5 Colony immunoscreening (Christian et al. J. Mol. Biol. (1992) 227, 711-718) (Felici et al. Gene (1993) 128, 21-27) 4.5 Plaque immunoscreening (Luzzago et al., Gene (1993) 128:51-57) (Felici et al., Methods Enzymol. (1996) 267:116-129) **5) Are there any World Wide Web (WWW) sites about molreps?** 5.1 http://www.bio.net/ - The BIOSCI web site itself (MOLREPS message archive) 5.2 http://bionmr1.rug.ac.be/chemistry/overview.html - A useful chemistry site 5.3 http://vesta.pd.com/ - Site for the journal MOLECULAR DIVERSITY 5.4 http://www.mrc-cpe.cam.ac.uk/imt-doc/vbase-home-page.html - Immunoglobulin v-gene database 5.5 http://molbio.info.nih.gov/molbio/desk.html - Molecular biologists desk reference 5.6 http://www.Kairos-scientific.com/ - Kairos scientific home page 5.7 http://www-lmmb.ncifcrf.gov/~toms/ - Tom Schneider's Theory of Molecular Machines 5.8 http://www.ebi.ac.uk/ - The European Bioinformatics Institute (EBI) 5.9 http://www.ebi.ac.uk/primers_home.html - PCR primers database at EBI 5.10 http://aminoacid.bri.nrc.ca:1125/ - Database of building blocks for library synthesis 5.11 http://vent.neb.com/neb/phd/phd.html - PHD commercial phage library at New England Biolabs > 5.12 ftp://ftp.netcom.com/pub/qu/quincicc/maxim.html > - Another commercial library source > This link seems to be broken. ------------------------------------------------------------------ Andrew Wallace, Ph.D, Queen's University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://www.qub.ac.uk/b&bchem/rbiochem.htm