From owner-repertoires@net.bio.net Thu Aug 01 23:00:00 1996 Path: biosci!queens-belfast.ac.uk!a.wallace From: a.wallace@queens-belfast.ac.uk (Andrew Wallace) Newsgroups: bionet.molecules.repertoires Subject: Postdoc available for chemist Date: 2 Aug 1996 06:03:19 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 32 Sender: daemon@net.bio.net Distribution: world Message-ID: <3201FCC6.2304@queens-belfast.ac.uk> NNTP-Posting-Host: net.bio.net The Queen's University of Belfast Postdoctoral Research Fellowship School of Chemistry This post, funded by the Engineering and Physical Sciences Research Council (UK), is available for 1 year from October 1996, to work on an interdisciplinary project involving the Schools of Chemistry and Biology and Biochemistry. The project concerns the use of solid phase synthetic methods to generate libraries of novel chemically modified amino acids and peptides. Applicants must hold an honours degree or equivalent in chemistry and a PhD in organic chemistry (or have submitted a thesis by 1 October 1996). Experience in organic synthesis is essential and experience of amino acid and peptide chemistry is desirable. Salary range: 14,319 - 15,987 pounds sterling per annum, placing depending on age, experience and qualifications. Applicants, quoting Ref. 96/F038, may obtain further particulars from the Personnel Office, The Queen's University of Belfast, BT7 1NN, Northern Ireland (UK), Fax: +44-1232-324944. Closing date: 9th August 1996 -- Note: please do not send email to me as I cannot forward messages on your behalf - apply directly to Personnel. -- ====================================================================== Andrew Wallace,Ph.D., Queen's University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://www.qub.ac.uk/b&bchem/rbiochem.htm ====================================================================== From owner-repertoires@net.bio.net Sun Aug 04 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Corrections to FAQ Date: 5 Aug 1996 12:33:34 +0100 Lines: 9 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4u4m6e$hao@mserv1.dl.ac.uk> MIME-Version: 1.0 X-Authentication: none Original-To: molreps@dl.ac.uk If anyone has any corrections to be made to the FAQ, please let me know. Andrew ------------------------------------------------------------------ Andrew Wallace, Ph.D, Queen's University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://www.qub.ac.uk/b&bchem/awpage/wallace.htm From owner-repertoires@net.bio.net Mon Aug 05 23:00:00 1996 Path: biosci!U.ARIZONA.EDU!jim From: jim@U.ARIZONA.EDU (Jin-seon Im) Newsgroups: bionet.molecules.repertoires Subject: about pcomb3 and pcom3H Date: 6 Aug 1996 11:54:34 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <199608061855.LAA40205@aruba.ccit.arizona.edu> NNTP-Posting-Host: net.bio.net hello fellas.. i'm trying to make library using pComb3/pcomb3H system have any idea which one is better ? and is there any place i can get the whole sequences in file? Thanks in advances jsim From owner-repertoires@net.bio.net Wed Aug 07 23:00:00 1996 Path: biosci!agate!news.ucdavis.edu!info.ucla.edu!csulb.edu!news.sgi.com!swrinde!cs.utexas.edu!howland.erols.net!vixen.cso.uiuc.edu!newsfeed.internetmci.com!in2.uu.net!EU.net!usenet2.news.uk.psi.net!uknet!usenet1.news.uk.psi.net!uknet!uknet!strath-cs!queens-belfast.ac.uk!queens-belfast.ac.uk!nntp Newsgroups: bionet.molecules.repertoires Subject: Self replicating peptides Message-ID: <3209FED4.760B@queens-belfast.ac.uk> From: Andrew Wallace Date: Thu, 08 Aug 1996 15:51:00 +0100 Nntp-Posting-Host: awall.bc.qub.ac.uk X-Mailer: Mozilla 2.0 (Macintosh; I; PPC) MIME-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Lines: 8 Check out today's (8th August) issue of Nature for an article on self-replicating coiled-coil peptides. Looks like an interesting follow-up job for a coiled-coil peptide library. -- ====================================================================== Andrew Wallace,Ph.D., Queen's University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://www.qub.ac.uk/b&bchem/awpage/wallace.htm ====================================================================== From owner-repertoires@net.bio.net Wed Aug 07 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Life on Mars Date: 8 Aug 1996 17:49:22 +0100 Lines: 16 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4ud5qi$s6d@mserv1.dl.ac.uk> MIME-Version: 1.0 Original-To: molreps@dl.ac.uk Nothing to do with combinatorial methods, but if you want to see the text of the famous paper about alleged fossilised lifeforms in a martian meteorite, Science have put out the full text of the paper on their web site, the URL is: http://www.aaas.org/science/mars/924/924.html Otherwise, wait until the 16th of August to see the paper version. Andrew -- ====================================================================== Andrew Wallace,Ph.D., Queen's University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://www.qub.ac.uk/b&bchem/awpage/wallace.htm ====================================================================== From owner-repertoires@net.bio.net Wed Aug 07 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Self replicating peptides Date: 8 Aug 1996 17:47:46 +0100 Lines: 10 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4ud5ni$s4n@mserv1.dl.ac.uk> MIME-Version: 1.0 Original-To: molreps@dl.ac.uk Check out today's (8th August) issue of Nature for an article on self-replicating coiled-coil peptides. Looks like an interesting follow-up job for a coiled-coil peptide library. Andrew -- ====================================================================== Andrew Wallace,Ph.D., Queen's University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://www.qub.ac.uk/b&bchem/awpage/wallace.htm ====================================================================== From owner-repertoires@net.bio.net Wed Aug 07 23:00:00 1996 Path: biosci!agate!news.ucdavis.edu!info.ucla.edu!nnrp.info.ucla.edu!csulb.edu!news.sgi.com!swrinde!newsfeed.internetmci.com!in3.uu.net!EU.net!usenet2.news.uk.psi.net!uknet!usenet1.news.uk.psi.net!uknet!uknet!strath-cs!queens-belfast.ac.uk!queens-belfast.ac.uk!nntp Newsgroups: bionet.molecules.repertoires Subject: Life on Mars Message-ID: <3209FFDE.6055@queens-belfast.ac.uk> From: Andrew Wallace Date: Thu, 08 Aug 1996 15:55:26 +0100 Nntp-Posting-Host: awall.bc.qub.ac.uk X-Mailer: Mozilla 2.0 (Macintosh; I; PPC) MIME-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Lines: 15 Nothing to do with combinatorial methods, but if you want to see the text of the famous paper about alleged fossilised lifeforms in a martian meteorite, Science have put out the full text of the paper on their web site, the URL is: http://www.aaas.org/science/mars/924/924.html Otherwise, wait until the 16th of August to see the paper version. Andrew -- ====================================================================== Andrew Wallace,Ph.D., Queen's University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://www.qub.ac.uk/b&bchem/awpage/wallace.htm ====================================================================== From owner-repertoires@net.bio.net Thu Aug 08 23:00:00 1996 Path: biosci!PLANTPATH.WISC.EDU!mrs From: mrs@PLANTPATH.WISC.EDU (Mike Sussman) Newsgroups: bionet.molecules.repertoires Subject: Self replicating peptides Date: 9 Aug 1996 09:08:25 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Check out today's (8th August) issue of Nature for an article on self-replicating coiled-coil peptides. Looks like an interesting follow-up job for a coiled-coil peptide library. Andrew -- ====================================================================== Andrew Wallace,Ph.D., Queen's University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://www.qub.ac.uk/b&bchem/awpage/wallace.htm ====================================================================== From owner-repertoires@net.bio.net Wed Aug 21 23:00:00 1996 Path: biosci!rutgers!csn!nntp-xfer-1.csn.net!news.acsu.buffalo.edu!newsstand.cit.cornell.edu!portc01.blue.aol.com!news-res.gsl.net!news.gsl.net!hunter.premier.net!uunet!in3.uu.net!EU.net!Portugal.EU.net!news.rccn.net!scsing.switch.ch!ubaclu.unibas.ch!bioftp.unibas.ch!daresbury!not-for-mail Newsgroups: bionet.molecules.repertoires Subject: EMBO course announcement and application form Message-ID: <4vhlqk$6rq@mserv1.dl.ac.uk> From: Andrew Wallace Date: 22 Aug 1996 14:03:16 +0100 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet MIME-Version: 1.0 X-Authentication: none Original-To: molreps@dl.ac.uk Lines: 196 EMBO/ICGEB Phage Display Course November 17-26 1996 This course is designed to give students an understanding of the use of pha= ge =0Adisplay in biological applications. In particular, students will be = given =0Ahands on training in the principles of creating a phage library, i= n the =0Aselection and subsequent screening and analysis of selected clones= .. A =0Adownstream use of cloned recombinant antibodies isolated from eithe= r phage =0Aantibody libraries or from hybridomas is in their intracellular = expression to =0Ainhibit the function of endogenous molecules. The use of = ectopic antibody =0Aexpression to inhibit protein function will be demonstr= ated. The course will =0Abe divided into four modules: 1=09Library creation 2=09Selection 3=09Screening and analysis 4=09Intracellular expression of antibodies (demonstration) Each module is divided into a number of different parts, and each student w= ill =0Ado at least one part from each module. =20 There will be extensive interactive discussion and all students will be =0A= expected to give a ten minute seminar on their work. Eligibility Students must have absoulute familiarity in the techniques of molecular =0A= biology, especially DNA cloning and PCR. Experience in the expression of = =0Arecombinant proteins would be an advantage. =20 Students who do not have experience in molecular biology will find the cour= se =0Atoo difficult to follow as no basic techniques will be explained. Students may be pre or post-doctoral, providing their experience is adequat= e. Students from industry or academia may apply, although different costs appl= y =0A(see below).=20 Students from EMBO member countries (Austria, Belgium, Czech Republic, =0AD= enmark, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Israel= , =0AItaly, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland, Turk= ey, =0AUnited Kingdom) working in EMBO member countries will be given prefe= rence, =0Aalthough applications will also be considered from students worki= ng abroad who =0Awill be returning to an EMBO member country, and from stud= ents who are =0Apresently working in EMBO member countries whatever their o= rigin. In =0Aaddition, two to three places will be reserved for students f= rom ICGEB =0Asignatory countries (Afghanistan, Algeria, Argentina, Banglade= sh, Bhutan, =0ABolivia, Bosnia & Herzegovina, Brazil, Bulgaria, Chile, Chin= a, Colombia, =0ACongo, Costa Rica, Croatia, Cuba, Ecuador, Egypt, F.R. Yugo= slavia, Georgia, =0AGhana, Greece, Hungary, India, Indonesia, Iran, Iraq, I= taly, Kyrgyzstan, =0AKuwait, Mauritania, Mauritius, Mexico, Morocco, Nigeri= a, Pakistan, Panama, =0APeru, Poland, Romania, Russia, Senegal, Slovenia, S= pain, Sri Lanka, Sudan, =0ASyria, T.F.Y.R. Macedonia, Thailand, Trinidad & = Tobago, Tunisia, Turkey, =0AUruguay, Venezuela, Viet Nam, Zaire). =20 Application The application form should be filled in and returned by 30 August 1996. = =20 One recommendation letter (sent by separate cover) in which the degree of = =0Aexperience in molecular biology is spelled out should also be provided. Successful applicants will be informed by 30 September 1996. Cost The cost per student will be 500.000 Italian lira (to be paid in advance). = =0AIndustrial participants must pay in addition to this, 1500 AU (ITL 3,19= 1,280 =0Aor US$ 1971.98) directly to the EMBO head office. The cost includes all accomodation and meals as well as the course costs. Grants Two grants of 500.000 ITL will be provided to two students in particular ne= ed. =0AThese students will also have their registration fee waived. Stude= nts wishing =0Ato be considered for these grants should explain their reaso= ns in an =0Aaccompanying letter. Outline of the course Library creation This module is divided in three: two practical parts and a theoretical part= .. =0AThe first is the creation of a phage antibody library, the second, th= e =0Acreation of a phage displaying recombinant cDNA clones and the last a = =0Adiscussion about peptide libraries. Students will do one of the two pra= ctical =0Aparts and follow the other. =20 1=09A phage antibody library will be made either from a naive source (e.g. = =0APBL) or from a hybridoma (hybridomas often contain more than the expecte= d =0Asingle heavy and light chains, and the creation of a limited library f= ollowed =0Aby selection is often the best way to clone the correct VH and V= L), using =0Aappropriate primers. =20 2=09The cDNA phage display module will involve the use of either gene 6, = =0Agene 3 display vectors or the pJuFu vector. =20 3=09A talk will be given about making phage peptide libraries. Selection This module will involve the selection of binding phage antibodies or phage= =0Apeptides using a number of different techniques. Each technique will b= e =0Aexplained and students will be divided into a number of different grou= ps to do =0Aone or more of each of the different selection methods. 1=09Solid phase: antigen bound to plastic. 2=09Biotinylated Ag with subsequent capture of the phage antibody / =0Abiot= inylated antigen using magnetic avidin beads 3=09Cellular (EGP2) 4=09Living column: the use of bacteria expressing antigen on their surface = =0Ato select binding phage antibodies. 5=09Serum peptide selection 6=09Theoretical talk on the use of alternative methods of selection Screening and analysis In this module students will characterise selected phage antibodies (select= ed =0Afrom a library, derived from hybridomas, or prepared in advance). Th= ree sorts =0Aof screening and analysis will be done: 1=09ELISA using phage 2=09ELISA using soluble scFv induced from infected bacteria 3=09The determination of affinity of selected antibodies (discussion and = =0Apractical). Intracellular expression of antibodies A downstream use of cloned recombinant antibodies isolated from either phag= e =0Aantibody libraries or from hybridomas is in their intracellular expres= sion to =0Ainhibit the function of endogenous molecules. The rationale for= ectopically =0Aexpressing antibodies within different cellular compartment= s to inhibit the =0Afuncion of endogenous proteins will be explained. This= will be followed by a =0Ademonstration of the expression of antibodies in different= cellular =0Acompartments (cytoplasm, nucleus, ER, mitochondria, secreted) = using the =0Aconfocal microscope. Student=D5s own samples Requests to bring samples by students will be considered by the organisers,= =0Aand should be indicated on the application form. =20 Such samples (if they are accepted) may take the form of hybridomas from wh= ich =0Astudents may wish to clone the light and heavy chain variable region= s. If =0Asamples are accepted, students must bring with them poly adenylat= ed mRNA and =0A1mg of antigen for use in selection and screening. =20 =20 -------------------------------page break----------------------------------= -- EMBO/ICGEB Phage Display Course: Application form=20 To be returned to A. Bradbury by 31 August. Tick appropriate spaces and ty= pe =0Aor write clearly Name, Institute and address Telephone=09=09=09=09Fax=09=09=09=09email Age=09=09Tenured position =09 Post-doctoral =09Ph.D. =0Astude= nt =20 Nationality=09=09=09=09 (member) EMBO ICGEB =20 Research experience Title of the ten minute talk you will give How will this course benefit your research? What you will be able to offer other course members? Two best publications Would you like to bring a sample? and if so what is the sample you would li= ke =0Ato bring? =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3DCUT HERE=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D ------------------------------------------------------------------ Andrew Wallace, Ph.D, Queen's University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://www.qub.ac.uk/b&bchem/awpage/wallace.htm From owner-repertoires@net.bio.net Thu Aug 22 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: EMBO course on phage display Date: 23 Aug 1996 13:04:45 +0100 Lines: 189 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4vk6ot$181@mserv1.dl.ac.uk> MIME-Version: 1.0 X-Authentication: none Original-To: molreps@dl.ac.uk EMBO/ICGEB Phage Display Course November 17-26 1996 This course is designed to give students an understanding of the use of phage display in biological applications. In particular, students will be given hands on training in the principles of creating a phage library, in the selection and subsequent screening and analysis of selected clones. A downstream use of cloned recombinant antibodies isolated from either phage antibody libraries or from hybridomas is in their intracellular expression to inhibit the function of endogenous molecules. The use of ectopic antibody expression to inhibit protein function will be demonstrated. The course will be divided into four modules: 1 Library creation 2 Selection 3 Screening and analysis 4 Intracellular expression of antibodies (demonstration) Each module is divided into a number of different parts, and each student will do at least one part from each module. There will be extensive interactive discussion and all students will be expected to give a ten minute seminar on their work. Eligibility Students must have absoulute familiarity in the techniques of molecular biology, especially DNA cloning and PCR. Experience in the expression of recombinant proteins would be an advantage. Students who do not have experience in molecular biology will find the course too difficult to follow as no basic techniques will be explained. Students may be pre or post-doctoral, providing their experience is adequate. Students from industry or academia may apply, although different costs apply (see below). Students from EMBO member countries (Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Israel, Italy, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland, Turkey, United Kingdom) working in EMBO member countries will be given preference, although applications will also be considered from students working abroad who will be returning to an EMBO member country, and from students who are presently working in EMBO member countries whatever their origin. In addition, two to three places will be reserved for students from ICGEB signatory countries (Afghanistan, Algeria, Argentina, Bangladesh, Bhutan, Bolivia, Bosnia & Herzegovina, Brazil, Bulgaria, Chile, China, Colombia, Congo, Costa Rica, Croatia, Cuba, Ecuador, Egypt, F.R. Yugoslavia, Georgia, Ghana, Greece, Hungary, India, Indonesia, Iran, Iraq, Italy, Kyrgyzstan, Kuwait, Mauritania, Mauritius, Mexico, Morocco, Nigeria, Pakistan, Panama, Peru, Poland, Romania, Russia, Senegal, Slovenia, Spain, Sri Lanka, Sudan, Syria, T.F.Y.R. Macedonia, Thailand, Trinidad & Tobago, Tunisia, Turkey, Uruguay, Venezuela, Viet Nam, Zaire). Application The application form should be filled in and returned by 15 September 1996. This can also be done by email to bradbury@icgeb.trieste.it One recommendation letter (sent by separate cover/email) in which the degree of experience in molecular biology is spelled out should also be provided. Successful applicants will be informed by 30 September 1996. Cost The cost per student will be 500.000 Italian lira (to be paid in advance). Industrial participants must pay in addition to this, 1500 AU (ITL 3,191,280 or US$ 1971.98) directly to the EMBO head office. The cost includes all accomodation and meals as well as the course costs. Grants Two grants of 500.000 ITL will be provided to two students in particular need. These students will also have their registration fee waived. Students wishing to be considered for these grants should explain their reasons in an accompanying letter. Outline of the course Library creation This module is divided in three: two practical parts and a theoretical part. The first is the creation of a phage antibody library, the second, the creation of a phage displaying recombinant cDNA clones and the last a discussion about peptide libraries. Students will do one of the two practical parts and follow the other. 1 A small phage antibody library will be made from RNA derived from a naive source (e.g. PBL). 2 The cDNA phage display module will involve the use of the pJuFu vector. 3 A talk will be given about making phage peptide libraries. Selection This module will involve the selection of binding phage antibodies, phage peptides or cDNAs using a number of different techniques. Each technique will be explained and students will be divided into a number of different groups to do one or more of each of the different selection methods. 1 Solid phase: antigen bound to plastic. 2 Biotinylated Ag with subsequent capture of the phage antibody / biotinylated antigen using magnetic avidin beads 3 Cellular (EGP2) 4 Theoretical talk on the use of alternative methods of selection Screening and analysis In this module students will characterise selected phage antibodies (selected from a library, derived from hybridomas, or prepared in advance). Two sorts of screening and analysis will be done: 1 ELISA using phage 2 ELISA using soluble scFv induced from infected bacteria Intracellular expression of antibodies A downstream use of cloned recombinant antibodies isolated from either phage antibody libraries or from hybridomas is in their intracellular expression to inhibit the function of endogenous molecules. The rationale for ectopically expressing antibodies within different cellular compartments to inhibit the funcion of endogenous proteins will be explained. This will be followed by a demonstration of the expression of antibodies in different cellular compartments (cytoplasm, nucleus, ER, mitochondria, secreted) using the confocal microscope. Student's own samples Requests to bring samples by students will be considered by the organisers, and should be indicated on the application form. Such samples (if they are accepted) may take the form of hybridomas from which students may wish to clone the light and heavy chain variable regions. If samples are accepted, students must bring with them poly adenylated mRNA and 1mg of antigen for use in selection and screening. EMBO/ICGEB Phage Display Course: Application form To be returned to A. Bradbury by 15 September. Tick appropriate spaces and type or write clearly Name, Institute and address Telephone Fax email Age Tenured position Post-doctoral Ph.D. student Nationality (member) EMBO ICGEB Research experience Title of the ten minute talk you will give How will this course benefit your research? What you will be able to offer other course members? Two best publications Would you like to bring a sample? and if so what is the sample you would like to bring? ------------------------------------------------------------------ Andrew Wallace, Ph.D, Queen's University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://www.qub.ac.uk/b&bchem/awpage/wallace.htm From owner-repertoires@net.bio.net Thu Aug 22 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Two postdoctoral positions available Date: 23 Aug 1996 12:55:14 +0100 Lines: 126 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4vk672$i9@mserv1.dl.ac.uk> MIME-Version: 1.0 X-Authentication: none Original-To: molreps@dl.ac.uk Two postdoctoral positions are available in our Institute One position is in the context of an EU grant on dendritic cells (DC); see www: http://www.medicine.ox.ac.uk/eunidi; http://www.unizh.ch/~vetvir/ and extended advert (below) which appeared in Nature (15th August 1996, vol. 382, classified page 23). Job descriptions: DC (lineage) specific genes will be identified by monoclonal antibodies (mAb) or other ligands and the cDNA product recognized by the ligand cloned using a recently developed filamentous phage system (see Gene, 137, 69, 1993). cDNA libraries from murine and human DC (DC lines or, freshly isolated) are available and are presently being completed. One position is on Ig repertoire cloning and on the development of novel peptide libraries. The aim is to obtain improved ligands for any desired molecule or the creation of mimitopes. The postdoctoral position would require construction of libraries and biophysical characterization of the libraries by binding phage to selected antigens or ligands. The work on both positions makes use of the same filamentous phage systems and a close collaboration between the two postdoctoral fellows is expected. We are seeking individuals with a strong background in molecular genetics and /or protein chemistry (quantitative ligand receptor interaction) to fill the positions. Both positions are for one year and can be extended up to three years. Both salaries are app. sFR. 80'000 (ECU 53'000) as set by the Swiss National Science Foundation. ------------------------------------------------ Nature advert (15th August 1996, vol. 382, classified page 23; [extended version]). POSTDOCTORAL POSITIONS COMMISSION OF THE EUROPEAN COMMUNITIES Training and Mobility of Researchers Vacancies exist for twelve (12) postdoctoral positions within a recently announced EC TMR Research Network entitled "European Union Network for Investigation of Dendritic Cell Immunotherapy (EUNIDI) for Induction of Anti-Viral and Anti-Tumor Immunity and Transplantation Tolerance". The network duration will be three years with a commencement date of 01 October 1996. The Network comprises 10 Partners in 7 European countries, each offering specific facilities and expertise essential to the overall project: 1. Jonathan Austyn (Network Coordinator), University of Oxford, UK. Tel: 44 1865 221 281. Fax: 44 1865 688 76. E-mail: jon.austyn@nds.ox.ac.uk 2. John Shields, Cantab Pharmaceuticals Research Ltd., Cambridge, UK. Tel: 44 1223 423 413. Fax: 44 1223 423 458. E-mail: jgs@cantab.demon.co.uk 3. Carl Figdor, University of Nijmegen, Netherlands. Tel: 31 24 3617600. Fax: 31 24 3540339. E-mail: c.figdor@dent.kun.nl 4. Antonio Lanzavecchia, Basel Institute for Immunology, Switzerland. Tel: 41 61 6051 326. Fax: 41 61 6051 222. E-mail: lanzavecchia@bii.ch 5. Muriel Moser, Free University of Brussels, Belgium. Tel: 32 2 650 98 50. Fax: 32 2 650 98 40. E-mail: mmoser@dbmdec5.ulb.ac.be 6. Paola Ricciardi-Castagnoli, University of Milan, Italy. Tel: 39 2 701 46 283. Fax: 39 2 701 46 373. E-mail: paola@farma8.csfic.mi.cnr.it 7. Gerold Schuler, University of Erlangen, Germany. Tel: 49 9131 85 3661. Fax: 49 9131 85 3854. E-mail: schuler@derma.med.uni-erlangen.de 8. Mark Suter, University of Zurich, Switzerland. Tel: 41 1 36 51537. Fax: 41 1 36 30140. E-mail: msuter@vetvir.unizh.ch 9. Penelope Mavromara, Hellenic Pasteur Institute, Athens, Greece. Tel: 30 1 64 31303. Fax: 30 1 64 23498 10. Cornelius Melief, University of Leiden, Netherlands. Tel: 31 71 52 63800. Fax: 31 71 52 16751. E-mail: ihbsecr@euronet..nl Each postdoctoral fellow will be based with one Partner but will have the opportunity to work with up to three different Partners during the course of the network duration. Training in a combination of molecular, cellular, and in vivo techniques can be anticipated. Further information can be obtained on the World Wide Web at http://www.medicine.ox.ac.uk/eunidi (and at http://www2.cordis.lu/tmr/src/adphys1.htm) and from individual participants. Applicants should be researchers aged 35 years or less at the time of appointment (an allowance may be made for military service or child care); hold a doctoral degree or its equivalent or, alternatively having at least four years full time research experience at graduate level; and be Nationals of a Member State of the Community or of an Associated State, but not a national or recent resident of the state in which the Partner appointing the researcher is situated. The restrictions regarding nationality of the applicant applies for the TMR network but not for the postdoctoral positions in Switzerland. Applications, by curriculum vitae and including the names of three referees, should be made to the Network Coordinator, Dr Jonathan M Austyn, Nuffield Department of Surgery, University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DU, UK. Tel: +44-1865-221281. Fax: +44-1865-68876. E-mail: jon.austyn@nds.ox.ac.uk. The closing date for applications is 30 September 1996. Short-listed applicants may be invited for interview by one of the Partners ------------------------ ------------------------------------- For more details and applications please send information to: Mark Suter, University of Zurich, Switzerland. Tel: 41 1 36 51537. Fax: 41 1 36 30140. E-mail: msuter@vetvir.unizh.ch \|/ (o o) ________________________________oOo__(_)__oOo_________________________________ ___/\_ | Mark Suter mailto:msuter@vetvir.unizh.ch / o \/| | University Inst.for Virology http://www.unizh.ch/vetvir / _| | Winterthurerstr. 266a Telephone: (+41) 1 6358717 /_/\__/-\/ | 8057 Zurich SWITZERLAND Faximile : (+41) 1 6358911 ______________________________________________________________________________ If it wasn't for Windows, we wouldn't have anything to compare Mac's to ______________________________________________________________________________ From owner-repertoires@net.bio.net Thu Aug 22 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!swrinde!newsfeed.internetmci.com!news.compuserve.com!ix.netcom.com!news From: spilamus@ix.netcom.com (spilamus) Newsgroups: bionet.molec-model,bionet.molecules.peptides,bionet.molecules.repertoires,bionet.mycology,bionet.n2-fixation Subject: money - money in the mail.wps [1/1] Date: 23 Aug 1996 03:22:46 GMT Organization: Your Organization Lines: 138 Message-ID: <4vj866$6bm@dfw-ixnews6.ix.netcom.com> NNTP-Posting-Host: ftw-tx2-01.ix.netcom.com Mime-Version: 1.0 Content-Type: Application/octet-stream; name=money in the mail.wps Content-Transfer-Encoding: Base64 X-NETCOM-Date: Thu Aug 22 10:22:46 PM CDT 1996 X-Newsreader: WinVN 0.99.5 Xref: biosci bionet.molec-model:1117 bionet.molecules.peptides:420 bionet.molecules.repertoires:217 bionet.mycology:4513 0M8R4KGxGuEAAAAAAAAAAAAAAAAAAAAAPgADAP7/CQAGAAAAAAAAAAAAAAAB AAAAAQAAAAAAAAAAEAAAAgAAAAEAAAD+////AAAAAAAAAAD///////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// ///////////////////////9////CgAAAP7///8EAAAABQAAAAYAAAAHAAAA CAAAAAkAAAD+/////v////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// /////////////////////////////////////////////1IAbwBvAHQAIABF AG4AdAByAHkAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAWAAUA//////////8DAAAAwtvNKOIKzhGimgCqAEoacgAAAAAA AAAAAAAAAACJNTlvkLsBAwAAAMAMAAAAAAAATQBhAHQATwBTAFQAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AA4AAQH/////BAAAAP////8AAAAAAAAAAAAAAAAAAAAAAAAAAGAuAzlvkLsB IMAkOW+QuwEAAAAAAAAAAAAAAABNAE0AAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAABgACAf// /////////////wAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAOAAAAAAAAAE0ATgAwAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAIAAIBAgAAAAEAAAD/ ////AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAQAAAPQL AAAAAAAA/v///wIAAAADAAAABAAAAAUAAAAGAAAABwAAAAgAAAAJAAAACgAA AAsAAAAMAAAADQAAAA4AAAAPAAAAEAAAABEAAAASAAAAEwAAABQAAAAVAAAA FgAAABcAAAAYAAAAGQAAABoAAAAbAAAAHAAAAB0AAAAeAAAAHwAAACAAAAAh AAAAIgAAACMAAAAkAAAAJQAAACYAAAAnAAAAKAAAACkAAAAqAAAAKwAAACwA AAAtAAAALgAAAC8AAAAwAAAA/v///zIAAAD+//////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////9ORAAAAQAAAAAAAAAEANDPEeChsRrh AAAAAAAAAAAAAAAAAAAAAD4AAwD+/wkABgAAAAAAAAAAAAAAAQAAAAEABv51 U28GAABvBgAAoAWwE1SbAQDQAgAAAAAAAQAAAgEAAAABAAB1BwAA9AsAAAAJ AAAKAAoJAAAQAAAAAAAAAAAAAAAAAAAAAAAAABoJAAAAABoJAAAAABoJAAAA ABoJAAASAKAFoAUIBwgH4D3QLwEAAAAAANAC3AQAAGQAAAAaCQAAAAD///// AAAAAAMI0AIAAAAAAABzBwAAAQB0AYAKAAAaCQAAAAAsCQAAVAEAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA0KDQpPa2F5LCBJkm0gc2Nh bm5pbmcgbmV3cyBncm91cHMgYW5kIGtlZXAgY29taW5nIGFjcm9zcyBhcnRp Y2xlcyBvbiBob3cgdG8gbGVnYWxseSB0dXJuICQ1IGludG8gJDUwMDAwLiBz b3VuZHMgdG8gZ29vZCB0byBiZSB0cnVlIHRvIG1lIGJ1dCwgSSByZWFkIG9u ZSBhZnRlciBhbm90aGVyIGFuZCBkZWNpZGUsIGhleSwgaXSScyAkNSwgZml2 ZSBzdGFtcHMsIGFuZCBhIGxpdHRsZSBiaXQgb2YgbXkgdGltZS4gV2hhdCBj YW4gaXQgaHVydCB0byBnaXZlIGl0IGEgdHJ5PyBTbywgd2h5IGRvbpJ0IHlv dSBnaXZlIGl0IGEgY2hhbmNlIHdpdGggbWUgPyBGb2xsb3cgdGhlIGluc3Ry dWN0aW9ucyBiZWxvdyBhbmQgbGV0knMgbWFrZSBzb21lIG1vbmV5ICEgDQoN CjEpIFdyaXRlIJMgUGxlYXNlIGFkZCBteSBuYW1lIHRvIHlvdXIgbGlzdJQg b24gZml2ZSBzaGVldHMgb2YgcGFwZXIuICggdGhpcyBjcmVhdGVzIGEgc2Vy dmljZSBhbmQgdGh1cyBtYWtlcyB0aGlzIHBsYW4gbGVnYWwgKSBXcmFwIG9u ZSBkb2xsYXIgaW50byBlYWNoIG5vdGUgYW5kIG1haWwgb25lIHRvIGVhY2gg cGVyc29uIG9uIHRoZSBsaXN0IGJlbG93LiANCg0KMikgUmV2aXNlIHRoZSBs aXN0IG9mIGZpdmUgbmFtZXMgLiBUbyBkbyB0aGlzIG1vdmUgIyAyIHBlcnNv biB0byAjMSwgIzMgcGVyc29uIHRvICMyLCAjNCBwZXJzb24gdG8gIzMsIGFu ZCAjNSBwZXJzb24gdG8gIyA0LiBGaW5hbGx5ICxhZGQgeW91ciBuYW1lIHRv IHRoZSBsaXN0IGluIHRoZSAjNSBzcGFjZS4gDQoNCjMpUG9zdCB0aGlzIG1l c3NhZ2UgKCByZXZpc2VkIGJ5IHlvdSApdG8gYXMgbWFueSBuZXdzZ3JvdXBz IGFzIHBvc3NpYmxlLiBSZW1lbWJlciwgdGhlIG1vcmUgbmV3c2dyb3VwcyB5 b3UgcG9zdCB0byB0aGUgbW9yZSByZXNwb25zZSB5b3Ugd2lsbCBnZXQuIA0K DQo0KSBTaXQgYmFjayBhbmQgd2FpdC4gVGhleSBzYXkgdGhhdCB0aGUgbW9u ZXkgd2lsbCB0cmlja2xlIGluIGF0IGZpcnN0LCB0aGVuIJFzdG9ybZIgeW91 ciBtYWlsYm94LiANCg0KTm90ZTogS2VlcCBhIGxpc3Qgb2YgZXZlcnlvbmUg dGhhdCBzZW5kcyB5b3UgYSBkb2xsYXIgYW5kIGtlZXAgYW4gZXllIG9uIHBv c3RpbmdzLiBZb3UgY2FuIGNoZWNrIHRvIG1ha2Ugc3VyZSBldmVyeW9uZSBp cyCTcGxheWluZyBmYWlylCAuIFJlbWVtYmVyLCBob25lc3R5IGlzIHRoZSBi ZXN0IHRoaW5nIHdlIGhhdmUgZ29uZyBmb3IgdXMgb24gdGhpcyBwbGFuICEg DQoNClRoZSBOYW1lIExpc3Q6ICAocmV2aXNlZCAwOC8yMS85NikNCihiZSBz dXJlIHRvIGRvdWJsZSBjaGVjayB5b3VyIGFkZHJlc3NlcyBiZWZvcmUgcG9z dGluZyAhKQ0KDQoxKSBNaWtlIERpb25pcw0KMTAyMTAgIEkgU3VubnkgTGFr ZSBQbGFjZQ0KQ29ja2V5c3ZpbGxlLCBNRCAyMTAzMA0KDQoyKSBKIFJvYmVy dCBHYWxsb3dheQ0KMjA4NSBIb2xseXdvb2QgQXZlLg0KU0xDLCBVVCAgODQx MDgNCg0KMykgTWF0dCBIaWVybA0KVzE4OCBTODY0MCBCcm9va2UgTGFuZQ0K TXVza2VnbywgV0kgICA1MzE1MA0KDQo0KUpvZSBKIEJ1ZGlvbiBJVg0KNjcy OSBTd2VldCBCdXNoIENvdXJ0DQpTeWx2YW5pYSwgT0ggNDM1NjANCg0KNSlN IEwgUm9iaW5zb24NCjM3MDIgQWxleGFuZHJpYSBEcml2ZQ0KQXJsaW5ndG9u IFR4LCA3NjAxNQ0KDQoNCgAAAAAAAAAAAAAAAAEAAE0BAABVAQAAdQcAAHt3 ewAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAADAggQAwAIEAMAAQAAAgEAAAQBAAB4AgAAegIAAEkDAABL AwAA/gMAAAAEAACVBAAAlwQAAPwEAAD+BAAA0gUAANQFAAD4BQAAMwYAADUG AABFBgAAYAYAAHgGAAB6BgAAcXEAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA0A AAAPCAAC4BDAIQECFXoGAACQBgAApQYAALUGAAC3BgAAxgYAAN4GAADzBgAA 9QYAAAgHAAAfBwAAMwcAADUHAABFBwAAXAcAAHEHAABzBwAAdQcAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAARAAEAAHUHAAAPAAABAAB6BgAAdQcAABAAEQAQEA9UaW1lcyBOZXcg Um9tYW4AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAADQAgAA eAAAAAAAAAAADQAAAAACAAAABAAAAAAAAAAAAAAAoAUAAKAFAAAIBwAACAcA AOA9AADQLwAA0AIAANwEAACgBQAAoAUAAAgHAAAIBwAA4D0AANAvAADQAgAA 3AQAAAEAAAAAAAAAAAAAAAAAAABkAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA0M8R 4KGxGuEAAAAAAQD+/wMKAAD/////wtvNKOIKzhGimgCqAEoachAAAABNaWNy b3NvZnQgV29ya3MADQAAAE1TV29ya3NXUERvYwAAAAAA9DmycQAAAAAAAAAA AAAAANDPEeChsRrhAAAAAAAAAAAAAAAAAAAAAD4AAwD+/wkABgAAAAAAAAAA AAD///////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// //////////////////////////////////////////////////////////// /////////wEAQwBvAG0AcABPAGIAagAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAASAAIA////////////////AAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAMQAAAFUAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAD///////////////8AAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAP///////////////wAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAA////////////////AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA From owner-repertoires@net.bio.net Fri Aug 23 23:00:00 1996 Path: biosci!biosci!not-for-mail From: pnh@ncifcrf.gov (Paul N Hengen) Newsgroups: bionet.molbio.methds-reagnts,bionet.molecules.repertoires Subject: EMBO/ICGEB Phage Display Course Date: 23 Aug 1996 21:44:03 -0700 Organization: NCI-FCRDC Frederick Biomedical Supercomputing Center Lines: 198 Sender: daemon@net.bio.net Distribution: world Message-ID: <4vl1s1$sb72@ncisun1-nf0.ncifcrf.gov> NNTP-Posting-Host: net.bio.net Xref: biosci bionet.molbio.methds-reagnts:48361 bionet.molecules.repertoires:220 -- *** EMBO/ICGEB Phage Display Course November 17-26 1996 *** This course is designed to give students an understanding of the use of phage display in biological applications. In particular, students will be given hands on training in the principles of creating a phage library, in the selection and subsequent screening and analysis of selected clones. A downstream use of cloned recombinant antibodies isolated from either phage antibody libraries or from hybridomas is in their intracellular expression to inhibit the function of endogenous molecules. The use of ectopic antibody expression to inhibit protein function will be demonstrated. The course will be divided into four modules: 1 Library creation 2 Selection 3 Screening and analysis 4 Intracellular expression of antibodies (demonstration) Each module is divided into a number of different parts, and each student will do at least one part from each module. There will be extensive interactive discussion and all students will be expected to give a ten minute seminar on their work. Eligibility *********** Students must have absoulute familiarity in the techniques of molecular biology, especially DNA cloning and PCR. Experience in the expression of recombinant proteins would be an advantage. Students who do not have experience in molecular biology will find the course too difficult to follow as no basic techniques will be explained. Students may be pre or post-doctoral, providing their experience is adequate. Students from industry or academia may apply, although different costs apply (see below). Students from EMBO member countries (Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Israel, Italy, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland, Turkey, United Kingdom) working in EMBO member countries will be given preference, although applications will also be considered from students working abroad who will be returning to an EMBO member country, and from students who are presently working in EMBO member countries whatever their origin. In addition, two to three places will be reserved for students from ICGEB signatory countries (Afghanistan, Algeria, Argentina, Bangladesh, Bhutan, Bolivia, Bosnia & Herzegovina, Brazil, Bulgaria, Chile, China, Colombia, Congo, Costa Rica, Croatia, Cuba, Ecuador, Egypt, F.R. Yugoslavia, Georgia, Ghana, Greece, Hungary, India, Indonesia, Iran, Iraq, Italy, Kyrgyzstan, Kuwait, Mauritania, Mauritius, Mexico, Morocco, Nigeria, Pakistan, Panama, Peru, Poland, Romania, Russia, Senegal, Slovenia, Spain, Sri Lanka, Sudan, Syria, T.F.Y.R. Macedonia, Thailand, Trinidad & Tobago, Tunisia, Turkey, Uruguay, Venezuela, Viet Nam, Zaire). Application *********** The application form should be filled in and returned by 15 September 1996. This can also be done by email to bradbury@icgeb.trieste.it One recommendation letter (sent by separate cover/email) in which the degree of experience in molecular biology is spelled out should also be provided. Successful applicants will be informed by 30 September 1996. Cost **** The cost per student will be 500.000 Italian lira (to be paid in advance). Industrial participants must pay in addition to this, 1500 AU (ITL 3,191,280 or US$ 1971.98) directly to the EMBO head office. The cost includes all accomodation and meals as well as the course costs. Grants ****** Two grants of 500.000 ITL will be provided to two students in particular need. These students will also have their registration fee waived. Students wishing to be considered for these grants should explain their reasons in an accompanying letter. Outline of the course ********************* Library creation This module is divided in three: two practical parts and a theoretical part. The first is the creation of a phage antibody library, the second, the creation of a phage displaying recombinant cDNA clones and the last a discussion about peptide libraries. Students will do one of the two practical parts and follow the other. 1 A small phage antibody library will be made from RNA derived from a naive source (e.g. PBL). 2 The cDNA phage display module will involve the use of the pJuFu vector. 3 A talk will be given about making phage peptide libraries. Selection This module will involve the selection of binding phage antibodies, phage peptides or cDNAs using a number of different techniques. Each technique will be explained and students will be divided into a number of different groups to do one or more of each of the different selection methods. 1 Solid phase: antigen bound to plastic. 2 Biotinylated Ag with subsequent capture of the phage antibody /biotinylated antigen using magnetic avidin beads 3 Cellular (EGP2) 4 Theoretical talk on the use of alternative methods of selection Screening and analysis In this module students will characterise selected phage antibodies (selected from a library, derived from hybridomas, or prepared in advance). Two sorts of screening and analysis will be done: 1 ELISA using phage 2 ELISA using soluble scFv induced from infected bacteria Intracellular expression of antibodies A downstream use of cloned recombinant antibodies isolated from either phage antibody libraries or from hybridomas is in their intracellular expression to inhibit the function of endogenous molecules. The rationale for ectopically expressing antibodies within different cellular compartments to inhibit the funcion of endogenous proteins will be explained. This will be followed by a demonstration of the expression of antibodies in different cellular compartments (cytoplasm, nucleus, ER, mitochondria, secreted) using the confocal microscope. Student's own samples Requests to bring samples by students will be considered by the organisers, and should be indicated on the application form. Such samples (if they are accepted) may take the form of hybridomas from which students may wish to clone the light and heavy chain variable regions. If samples are accepted, students must bring with them poly adenylated mRNA and 1mg of antigen for use in selection and screening. EMBO/ICGEB Phage Display Course: Application form To be returned to A. Bradbury by 15 September. Tick appropriate spaces and type or write clearly Name, Institute and address Telephone Fax email Age Tenured position Post-doctoral Ph.D. student Nationality (member) EMBO ICGEB Research experience Title of the ten minute talk you will give How will this course benefit your research? What you will be able to offer other course members? Two best publications Would you like to bring a sample? and if so what is the sample you would like to bring? Please send to: Andrew Bradbury SISSA c/o ICGEB Area di Ricerca Padriciano 99 Trieste 34012 Italy Tel +39 40 398995 Fax +39 40 398991 email: bradbury@icgeb.trieste.it -- ******************************************************************************* * Paul N. Hengen, Ph.D. /--------------------------/* * National Cancer Institute |Internet: pnh@ncifcrf.gov |* * Laboratory of Mathematical Biology | Phone: (301) 846-5581 |* * Frederick Cancer Research and Development Center| FAX: (301) 846-5598 |* * Frederick, Maryland 21702-1201 USA /--------------------------/* * - - - Methods FAQ list -> ftp://ftp.ncifcrf.gov/pub/methods/FAQlist - - - * * - TIBS column archive -> http://www-lmmb.ncifcrf.gov/~pnh/readme.html - - * * - The BEST Molecular Biology HomePage -> http://www-lmmb.ncifcrf.gov/~pnh/ * ******************************************************************************* From owner-repertoires@net.bio.net Tue Aug 27 23:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molecules.repertoires Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 28 Aug 1996 02:00:09 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199608280900.CAA18988@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net