From owner-repertoires@net.bio.net Sun Mar 02 22:00:00 1997 Path: biosci!IR2CBM.CNRS-MRS.FR!chames From: chames@IR2CBM.CNRS-MRS.FR (Patrick CHAMES) Newsgroups: bionet.molecules.repertoires Subject: (none) Date: 3 Mar 1997 03:42:39 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: <9703031141.AA00963@ir2cbm.cnrs-mrs.fr> NNTP-Posting-Host: net.bio.net does anybody know if during a selection of phages (phagemids) on immunotube, a too high phage concentration can be a problem (for example 10(13) phages in one ml of milk/pbs). thank you for your responses ------------------------------------ Patrick CHAMES CNRS-LIDSM 31 chemin Joseph Aiguier 13402 MARSEILLE cedex 20 tel 04 91 16 45 61 fax 04 91 71 21 24 ------------------------------------- From owner-repertoires@net.bio.net Wed Mar 05 22:00:00 1997 Path: biosci!daresbury!lyra.csx.cam.ac.uk!nntpfeed.doc.ic.ac.uk!sunsite.doc.ic.ac.uk!netcom.net.uk!dispatch.news.demon.net!demon!newsfeed.nacamar.de!news.maxwell.syr.edu!worldnet.att.net!news.sprintlink.net!news-peer.sprintlink.net!news-pull.sprintlink.net!news.sprintlink.net!news-stk-3.sprintlink.net!moonbeam.aecom.yu.edu!post!moran From: "Justin K. Moran" Newsgroups: bionet.molecules.repertoires Subject: b-galactosidase cleavage Date: Thu, 6 Mar 1997 12:41:28 -0500 Organization: Albert Einstein College of Medicine Message-ID: NNTP-Posting-Host: post.aecom.yu.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Sender: moran@post Lines: 10 Hello, I am not sure if this is the correct group for this question, but I thought I would try anyways. Does anybody know if modifications to C-6 of galactose (ie reductive amination) will effect cleavage by b-galactosidase? Thanks for any help you can give. Justin Moran From owner-repertoires@net.bio.net Sun Mar 09 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!news.sgi.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!feed1.news.erols.com!howland.erols.net!ais.net!ameritech.net!uunet!in3.uu.net!199.0.65.182!news-in.tiac.net!posterchild!news@tiac.net From: chi@healthtech.com (Cambridge Healthtech Institute) Newsgroups: bionet.molecules.repertoires Subject: Fourth Annual HIGH-THROUGHPUT SCREENING Date: Mon, 10 Mar 1997 19:44:47 GMT Organization: Cambridge Healthtech Institute Lines: 39 Message-ID: <5g1o64$rh9@news-central.tiac.net> Reply-To: chi@healthtech.com NNTP-Posting-Host: 207.60.238.8 X-Newsreader: Forte Free Agent 1.0.82 Fourth Annual HIGH-THROUGHPUT SCREENING June 25-27 Arlington, Virginia Primary Sponsors: Corning Costar Corporation and Molecular Devices Corporation Full Event Sponsor: Packard Instrument Company Following a joint day with MIPTech of presentations covering integrated screening programs, other specific topics of importance for drug development screening will be highlighted. Developments related to target selection, assay design, sources of compounds for screening, including natural products and small molecule combinatorial libraries, rapid compound characterization and informatics will each be covered. Novel approaches and perspectives on improving performance will be highlighted. A major trade exhibit and vendor workshops will be featured throughout the week. If you wish to receive more information, please contact: Cambridge Healthtech Institute 1037 Chestnut Street Newton Upper Falls, MA 02164 USA Phone: 617-630-1300 Fax: 617-630-1325 e-mail: chi@healthtech.com http://www.healthtech.com/conferences/ ______________________________ Cambridge Healthtech Institute 1037 Chestnut Street Newton Upper Falls, MA 02164 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ tel: 617.630.1300 fax: 617.630.1325 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ inquiries@healthtech.com World Wide Web http://www.healthtech.com/conferences From owner-repertoires@net.bio.net Sun Mar 09 22:00:00 1997 Path: biosci!tanabe.co.jp!ryo From: ryo@tanabe.co.jp (Ryo Shimizu) Newsgroups: bionet.molecules.repertoires Subject: me Date: 9 Mar 1997 18:59:28 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 62 Sender: daemon@net.bio.net Distribution: world Message-ID: <199703100259.LAA19915@tanabe.co.jp> NNTP-Posting-Host: net.bio.net Dear Netters, We are pleased to announce that Japan Combinatorial Focus Group, JCCF, holds the 4th JCCF Meeting on March 31-April 1 at Tokyo Institute of Technology in Tokyo, Japan. The program is as follows; March 31st Opening Remarks Takashi Takahashi (Tokyo Institute of Technology) Solid Phase Synthesis and Combinatorial Chemistry K. C. Nicolaou (The Scripps Research Institute) Reaction and Structure Templates in Combinatorial Chemistry Robert W. Armstrong (University of California, Los Angeles) Overviews of Recent Advances in Combinatorial Chemistry and Highthrouput Screening Yoshihisa Inoue (Green Cross Corp.) Hisashi Arikuni (Japan Science & Technology Corp.) Epitope analysis of viral proteins using synthetic random peptide libraries: Identification of peptides mimicking the antigenicity and immunogenicity of discontinuous epitopes Yoshihiro Hirabayashi (Tokyo Metropolitan Institute for Neuroscience) An Integrated Mass Spectrometry Screening Tool for Combinatorial Chemistry Ramesh Rao (Finnigan) Mixer April 1st Encoded Combinatorial Chemistry: A Tool for the Study of Selective Intermolecular Interactions Helma Wennemers (Nagoya University) Combinatorial Libraries of Molecular Forceps - Chemistry and beyond? Hans Peter Nestler (Cold Spring Habor Laboratory) DirectedDiversity Technology for the Property Refinement of Combinatorial Libraries F. Raymond Salemme (3-Dimensional Pharmaceuticals) Purification and Quality Control Issues Involved in the Production and High Throughput Screening of Combinatorial Libraries Jeff W. Paslay (PANLABS) Ultra-High-Throughput-Screening: Gene to Lead Harry Stylli (Aurora Biosci.Corp.) Integration of HTS and Combinatorial Chemistry for Drug Discovery Mark J. Suto (Signal Pharmaceuticals) Closing Remarks Koichi Fukase (Osaka University) For details, please see the JCCF homepage. The URL is http://www.orgsyn.riken.go.jp/CombiChem.html. Contact Japan Combinatorial Chemistry Focus Group Office c/o Kinki Chemical Society, Japan 1-8-4 Utsubo-honmachi, Nishi-ku, Osaka 550, Japan FAX:+81(Japan)-6-443-6685 Email: jccf@yo.rim.or.jp Ryo Shimizu, Ph.D. Head, Rational Drug Design, Lead Generation Research Laboratory, Tanabe Seiyaku Co., Ltd., Yodogawa, Osaka 532, JAPAN From owner-repertoires@net.bio.net Tue Mar 11 22:00:00 1997 Path: biosci!agate!newsgate.cuhk.edu.hk!news-hk.gsl.net!news.gsl.net!cyclic.gsl.net!news.gsl.net!news.maxwell.syr.edu!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!rill.news.pipex.net!pipex!netcom.net.uk!peer.news.u-net.net!yama.mcc.ac.uk!io.salford.ac.uk!aber!bath.ac.uk!dcl-cs!strath-cs!queens-belfast.ac.uk!queens-belfast.ac.uk!nntp Newsgroups: bionet.molecules.repertoires Subject: Update on moderation Message-ID: <331FE810.3BBB@qub.ac.uk> From: Andrew Wallace Date: Fri, 07 Mar 1997 10:04:00 +0000 Reply-To: a.wallace@queens-belfast.ac.uk Organization: Queens University Belfast Nntp-Posting-Host: awall.bc.qub.ac.uk X-Mailer: Mozilla 3.0 (Macintosh; I; PPC) MIME-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Lines: 14 Sorry to everyone about the junk mail that is still getting through, this newsgroup is not moderated yet. Dave Kristofferson and his team at BioSci are working on this so we can go ahead with moderation soon. Please be patient for another week or so and we will shortly have a junk mail-free newsgroup. Also, the FAQ is now accessable via the BioSci web page http://www.bio.net/hypermail/MOLECULAR-REPERTOIRES/ so feel free to check this out. Andrew -- ================================================================== Andrew Wallace,Ph.D., Queens University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://web.qub.ac.uk/bb/awpage/wallace.html ================================================================== From owner-repertoires@net.bio.net Wed Mar 19 22:00:00 1997 Path: biosci!biosci!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Moderation Date: 20 Mar 1997 11:23:25 -0800 Organization: Queens University Belfast Lines: 12 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <3331290C.544C@qub.ac.uk> Reply-To: a.wallace@queens-belfast.ac.uk NNTP-Posting-Host: net.bio.net This is just to let everyone know that the molreps group is now moderated, so the junk mails should finally stop. Andrew -- ================================================================== Andrew Wallace,Ph.D., Queens University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://web.qub.ac.uk/bb/awpage/wallace.html ================================================================== From owner-repertoires@net.bio.net Thu Mar 20 22:00:00 1997 Path: biosci!biosci!not-for-mail From: Antoine VEKRIS Newsgroups: bionet.molecules.repertoires Subject: high phages concentrations Date: 21 Mar 1997 03:55:04 -0800 Organization: Molecular pathology and gene therapy laboratory Lines: 34 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <3332829F.406D@u-bordeaux2.fr> Reply-To: Antoine.Vekris@pmtg.u-bordeaux2.fr NNTP-Posting-Host: net.bio.net > Patrick CHAMES (chames@IR2CBM.CNRS-MRS.FR) asked > does anybody know if during a selection of phages (phagemids) on > immunotube, a too high phage concentration can be a problem (for > example > 10(13) phages in one ml of milk/pbs). I am using pSKAN phagemids for selection of mimotopes, and YES! we had some problems when high concentrations of phages are used. To avoid that, and be able anyway to screen high numbers of phages I am using a coated tube spliced in two and immersed into a recipient containing a low dilution of phages, stirred gently. Concentrations are always lower the 10e+11 phages per ml. That works fine and decrease the number of selections from 6 to 3, to have the same enrichment. If you want to work "in" the tube you can just generate a flow, with a peristaltic pump. Depending of your target, some other carrier may be more usefull then milk. For cellular antigens I use rather horse serum as the saturating agent as well as carrier of the phages. The background decrease drastically. Good selections and high affinities fellows Antoine VEKRIS Molecular pathology and gene therapy laboratory University of Bordeaux II 146, rue Leo Saignat FR-33076 Bordeaux cedex Phone +33 (0)5 57571373, fax +33 (0)5 56983348, e-mail vekris@u-bordeaux2.fr From owner-repertoires@net.bio.net Mon Mar 24 22:00:00 1997 Path: biosci!biosci!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Duplicate postings Date: 25 Mar 1997 05:37:32 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5h8ebr$mqp@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net I am sorry for any duplicate postings of messages, but there are still some minor problems with the moderation setup of the newsgroup, so please bear with me until these are solved. Andrew ------------------------------------------------------------------ Andrew Wallace, Ph.D, Queens University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://web.qub.ac.uk/bb/awpage/wallace.html From owner-repertoires@net.bio.net Mon Mar 24 22:00:00 1997 Path: biosci!biosci!not-for-mail From: Antoine VEKRIS Newsgroups: bionet.molecules.repertoires Subject: Re: too high phage concentration Date: 25 Mar 1997 03:49:43 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 35 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5h8ds5$mbp@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net [sorry for any duplicate posting, there was an error when this was sent earlier - Andrew] > Patrick CHAMES (chames@IR2CBM.CNRS-MRS.FR) asked > does anybody know if during a selection of phages (phagemids) on > immunotube, a too high phage concentration can be a problem (for > example > 10(13) phages in one ml of milk/pbs). I am using pSKAN phagemids for selection of mimotopes, and YES! we had some problems when high concentrations of phages are used. To avoid that, and be able anyway to screen high numbers of phages I am using a coated tube spliced in two and immersed into a recipient containing a low dilution of phages, stirred gently. Concentrations are always lower the 10e+11 phages per ml. That works fine and decrease the number of selections from 6 to 3, to have the same enrichment. If you want to work "in" the tube you can just generate a flow, with a peristaltic pump. Depending of your target, some other carrier may be more usefull then milk. For cellular antigens I use rather horse serum as the saturating agent as well as carrier of the phages. The background decrease drastically. Good selections and high affinities fellows Antoine VEKRIS Molecular pathology and gene therapy laboratory University of Bordeaux II 146, rue Leo Saignat FR-33076 Bordeaux cedex Phone +33 (0)5 57571373, fax +33 (0)5 56983348, e-mail vekris@u-bordeaux2.fr From owner-repertoires@net.bio.net Mon Mar 24 22:00:00 1997 Path: biosci!biosci!not-for-mail From: Antoine VEKRIS Newsgroups: bionet.molecules.repertoires Subject: high phages concentrations (repost) Date: 25 Mar 1997 03:38:58 -0800 Organization: Molecular pathology and gene therapy laboratory Lines: 35 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <3332830F.406D@u-bordeaux2.fr> Reply-To: Antoine.Vekris@pmtg.u-bordeaux2.fr NNTP-Posting-Host: net.bio.net [sorry for any duplicate posting, there was an error when this was sent earlier - Andrew] > Patrick CHAMES (chames@IR2CBM.CNRS-MRS.FR) asked > does anybody know if during a selection of phages (phagemids) on > immunotube, a too high phage concentration can be a problem (for > example > 10(13) phages in one ml of milk/pbs). I am using pSKAN phagemids for selection of mimotopes, and YES! we had some problems when high concentrations of phages are used. To avoid that, and be able anyway to screen high numbers of phages I am using a coated tube spliced in two and immersed into a recipient containing a low dilution of phages, stirred gently. Concentrations are always lower the 10e+11 phages per ml. That works fine and decrease the number of selections from 6 to 3, to have the same enrichment. If you want to work "in" the tube you can just generate a flow, with a peristaltic pump. Depending of your target, some other carrier may be more usefull then milk. For cellular antigens I use rather horse serum as the saturating agent as well as carrier of the phages. The background decrease drastically. Good selections and high affinities fellows Antoine VEKRIS Molecular pathology and gene therapy laboratory University of Bordeaux II 146, rue Leo Saignat FR-33076 Bordeaux cedex Phone +33 (0)5 57571373, fax +33 (0)5 56983348, e-mail vekris@u-bordeaux2.fr From owner-repertoires@net.bio.net Wed Mar 26 22:00:00 1997 Path: biosci!biosci!not-for-mail From: markm@portal.vpharm.com (Mark Murcko) Newsgroups: bionet.molecules.repertoires Subject: Job Openings at Vertex Pharmaceuticals Date: 27 Mar 1997 04:59:22 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 56 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <9703271203.AA17086@portal.vpharm.com> NNTP-Posting-Host: net.bio.net Vertex Pharmaceutical Incorporated, combining the disciplines of biology, chemistry and biophysics, produces novel therapeutics that play a key role in the treatment of many diseases including cancer multidrug resistance, hemoglobin disorders, inflammation, and HIV infection and AIDS. We are seeking to fill the following positions: COMBINATORIAL CHEMISTS - ALL LEVELS Job Code - CC As a broadly experienced and highly creative synthetic chemist, you will apply your knowledge of organic reactions to automated synthesis of targeted compound libraries. You will interface closely with medicinal and peptide chemists and with the biological assay and computational chemistry groups. Your extensive experience with a variety of biologically relevant molecules, excellent communication skills, and strong desire to be part of the emerging field of automated synthetic chemistry will serve you well in this high visibility position. NON-PH.D APPLICANTS must have a strong background in synthetic organic chemistry with a minimum of two years of post-bachelor degree, full time bench laboratory experience and hands-on familiarity with programmable laboratory equipment. PH.D. APPLICANTS must also have a very strong background in synthetic organic chemistry, at least 2+ years post graduate experience, preferably in industry. A strong publication record is highly desirable. MEDICINAL CHEMISTS - ALL LEVELS Job Code - MC As a member of our medicinal chemistry group you will work closely with colleagues in combinatorial chemistry, biology, biophysics and computational chemistry in an intense and challenging interdisciplinary environment. Your desire to apply insights from a broad variety of areas to the process of drug design and strong commitment to experimental science are essential for this position. Applicants must have a degree in synthetic organic chemistry with at least 2+ years of post-graduate experience preferably in industry. Please send your resume to: Vertex Pharmaceuticals Incorporated Recruiting Manager Code: mol.reps 130 Waverly Street Cambridge, MA USA 02139 FAX: (617) 577-6645 e-mail: recruiting@vpharm.com. Visit our homepage @ http://www.vpharm.com From owner-repertoires@net.bio.net Thu Mar 27 22:00:00 1997 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.molecules.repertoires Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 28 Mar 1997 02:10:11 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 242 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <199703281000.CAA06003@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-repertoires@net.bio.net Thu Mar 27 22:00:00 1997 Path: biosci!biosci!not-for-mail From: jamesl@cambridge.org (James W. Larkin) Newsgroups: bionet.molecules.repertoires Subject: Request for Proposals Combinatorial Chemistry and High Throughput Date: 28 Mar 1997 02:08:39 -0800 Organization: Cambridge Symposia Lines: 50 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5h92nf$scs@news-central.tiac.net> NNTP-Posting-Host: net.bio.net Cambridge Healthtech Institutes First European Conference on Combinatorial Chemistry and High Throughput Screening December 4-5, 1997 Hotel Arts Barcelona Barcelona, Spain These two companion technologies are having a dramatic impact on efforts within the pharmaceutical and biotechnology industries worldwide to reduce the time and costs associated with initial lead identification and optimization. The conference will focus on recent developments by leading researches in small molecule combinatorial libraries, chemical and solid-phase synthesis, and purification. Talks focusing on the application of these technologies for product development as well as target applications will be covered. The second half of the technical program will detail advances in high throughput screening. The sessions will feature talks in target identification, novel assays, robotics and animation, informatics and data handling, as well as integrated programs. In addition, there will be workshops and a major trade exhibition during the meeting. This program offers a unique opportunity to access decision makers and researchers from around the world in these rapidly moving fields which are becoming essential for efficient drug discovery. Request for Proposals If you would like to be considered as a participant at this conference, please submit a proposal to include a title and brief summary of your presentation by April 7, 1997 to: Irene Phelan, Conference Director Phone: 617-630-1362 Fax: 617-630-1325 e-mail: iphelan@healthtech.com For companies interested in corporate sponsorship opportunites, in exhibiting or conducting workshops at this meeting, please contact: Jim MacNeil, Marketing Manager Phone: 617-630-1341 Fax: 617-630-1325 e-mail: jmacneil@healthtech. com Organized by Cambridge Healthtech Institute 1037 Chestnut Steet, Newton Upper Falls, MA 02164 USA http://www/healthtech.com/conferences/