From owner-repertoires@net.bio.net Tue Apr 01 23:00:00 1997 Path: biosci!biosci!not-for-mail From: hho@lpat.azm.nl (H. Hoogenboom) Newsgroups: bionet.molecules.repertoires Subject: Positions available Maastricht Date: 2 Apr 1997 04:47:53 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 75 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5htk9b$oor@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net The Antibody Engineering group CESAME, based in the Department of Pathology, University Hospital Maastricht, the Netherlands, has available positions for : Three Research Scientists Antibody Design and Engineering You will be involved in network research projects financed by the European Community as part of the 4th Biotechnology Framework. The research in Maastricht focuses on the development of strategies for the immunotherapy of cancer : (1) Recombinant bispecific antibodies for the treatment of B-cell non-Hodgkin's disease. This project involves the design and construction of 'diabodies', antibody fragments with two binding sites, using phage display and expression in bacteria (McGuinness et al. Nat Biotechnol 14, 1149-54, 1996). These proteins will be tested for their in vitro en in vivo T-cell-retargeting capacity. (2) MHC-peptide complex specific antibodies for tumor targeting. The candidate will study the possibility to derive human antibodies specific for recombinant MHC-Class I molecules complexed to tumor-associated peptides (similar to Andersen et al. Proc Natl Acad Sci USA 93, 1820-24, 1996, for class II). The tumor-targeting efficacy of these antibodies will be tested in close collaboration with other partners of the programme. (3) Intracellular antibodies : The candidate will be involved in designing new antibody scaffolds, creating synthetic phage antibody libraries and selecting high affinity ligands to certain tumor-associated intracellular targets (Persic et al Gene 187:1-16, 1997). You will be based in the Department of Pathology, University Hospital Maastricht, in the Netherlands, working in the multidisciplinary research team CESAME, in which antibody engineering en phage display are being used as key technologies to derive useful immunotargeting molecules (Hoogenboom, TIBTECH 15, 62-70; 1997). Applicants have a PhD in a relevant areas (molecular immunology, protein engineering, oncology) or equivalent experience; additional postdoctoral experience abroad is a major advantage. Expertise in molecular biology and familiarity with protein biochemistry are essential. Salary is according to age and experience within band 10 (to max Dfl. 6.092,-). Appointments are full time, for 2-3 years, and available within the next 4 months. More information about these positions may be obtained from dr. ir. H.R.J.M. Hoogenboom, tel. 31-43-3874630, or via E-mail : hho@lpat.azm.nl Please send, before the 1st of May, (1) Curriculum Vitae including details of two referees, (2) a 1-page description of research interests, and (3) a motivated ranking between the three cited projects, to dr. ir. H.R.J.M. Hoogenboom, Department of Pathology, Academisch Ziekenhuis Maastricht, P.O.. Box 5800, 6202 AZ Maastricht, the Netherlands; or FAX 31-43-3876613. ******************************** Hennie R.J.M. Hoogenboom CESAME at Department Pathology University Hospital Maastricht P. Debyelaan 25 P.O. Box 5800 6202 AZ MAASTRICHT THE NETHERLANDS tel. 00-31-43-387.4630 00-31-43-387.6612 FAX 00-31-43-387.6613 E-mail HHO@LPAT.AZM.NL ******************************** From owner-repertoires@net.bio.net Tue Apr 01 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "Janet Clench, Library, Tel:(39 6)91093220" Newsgroups: bionet.molecules.repertoires Subject: "Hi ho, hi ho, it's off to work we go,..." and have a Happy Easter Date: 2 Apr 1997 01:53:47 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 320 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5hgfa3$dcl@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net ******************************************************** SUBJECT: Combinatorial & Phage Libraries DATE: February 24, March 3,10,17 1997 ******************************************************* Finnern, R.; Pedrollo, E.; Fisch, I.; Wieslander, J.; Marks, J.D.; Lockwood, C.M.; Ouwehand, W.H. Human autoimmune anti-proteinase 3 scFv from a phage display library Clinical and Experimental Immunology 107: 2 (FEB 1997) 269-281 Combinatorial chemistry Drug Discovery Today 2: 2 (FEB 1997) 81-81 Eliseev, A.V.; Nelen, M.I. Use of molecular recognition to drive chemical evolution .1. Controlling the composition of an equilibrating mixture of simple arginine receptors Journal of the American Chemical Society 119: 5 (FEB 5 1997) 1147-1148 Harper, K.; Kerschbaumer, R.J.; Ziegler, A.; Macintosh, S.M.; Cowan, G.H.; Himmler, G.; Mayo, M.A.; Torrance, L. A scFv-alkaline phosphatase fusion protein which detects potato leafroll luteovirus in plant extracts by ELISA Journal of Virological Methods 63: 1-2 (JAN 1997) 237-242 Janniere, L.; Bidnenko, V.; McGovern, S.; Ehrlich, S.D. Replication terminus for DNA polymerase I during initiation of pAM beta 1 replication: Role of the plasmid-encoded resolution system Molecular Microbiology 23: 3 (FEB 1997) 525-535 Magliani, W.; Conti, S.; DeBernardis, F.; Gerloni, M.; Bertolotti, D.; Mozzoni, P.; Cassone, A.; Polonelli, L. Therapeutic potential of antiidiotype single chain antibodies with yeast killer toxin activity Nature Biotechnology 15: 2 (FEB 1997) 155-158 Studer, A.; Hadida, S.; Ferritto, R.; Kim, S.Y.; Jeger, P.; Wipf, P.; Curran, D.P. Fluorous synthesis: A fluorous-phase strategy for improving separation efficiency in organic synthesis Science 275: 5301 (FEB 7 1997) 823-826 Lee, B.H. Solid-phase synthesis of cyclooctadepsipeptide PF1022A analogs using a cyclization-cleavage method with oxime resin Tetrahedron Letters 38: 5 (FEB 3 1997) 757-760 Proulx, C.; Chartrand, P.; Roy, V.; Goldman, M.; Decary, F.; Rinfret, A. Human monoclonal Fab fragments recovered from a combinatorial library bind specifically to the platelet HPA-1a alloantigen on glycoprotein IIb-IIIa Vox Sanguinis 72: 1 (1997) 52-60 Uozumi, Y. Preparation of combinatorial library indexed by molecular tags Journal of Synthetic Organic Chemistry Japan 55: 1 (JAN 1997) 65-71 Harada, T.; Katada, J.; Tachiki, A.; Asari, T.; Iijima, K.; Uno, I.; Ojima, I.; Hayashi, Y. Development of the new potent non-peptide gpIIb/IIIa antagonist NSL- 95301 by utilizing combinatorial technique Bioorganic & Medicinal Chemistry Letters 7: 2 (JAN 21 1997) 209-212 Vanwetswinkel, S.; MarchandBrynaert, J.; Fastrez, J. Selection of the most active enzymes from a mixture of phage-displayed beta-lactamase mutants (Vol 6, pg 789, 1996) Bioorganic & Medicinal Chemistry Letters 7: 2 (JAN 21 1997) 239-239 Terrett, N. Combinatorial chemistry Drug Discovery Today 2: 1 (JAN 1997) 37-37 Hennecke, M.; Kola, A.; Baensch, M.; Wrede, A.; Klos, A.; Bautsch, W.; Kohl, J. A selection system to study C5a-C5a-receptor interactions: Phage display of a novel C5a anaphylatoxin, Fos-C5a(Ala27) Gene 184: 2 (JAN 15 1997) 263-272 Marasco, W.A. Intrabodies: Turning the humoral immune system outside in for intracellular immunization Gene Therapy 4: 1 (JAN 1997) 11-15 Davenport, M.P.; Smith, K.J.; Barouch, D.; Reid, S.W.; Bodnar, W.M.; Willis, A.C.; Hunt, D.F.; Hill, A.V.S. HLA class I binding motifs derived from random peptide libraries differ at the COOH terminus from those of eluted peptides Journal of Experimental Medicine 185: 2 (JAN 20 1997) 367-371 Kuebler, P.J.; Nixon, D.F. Cytotoxic T cell induction with ratchet peptide libraries Vaccine 14: 17-18 (DEC 1996) 1664-1670 Downs, G.M.; Barnard, J.M. Techniques for generating descriptive fingerprints in combinatorial libraries Journal of Chemical Information and Computer Sciences 37: 1 (JAN- FEB 1997) 59-61 Warr, W.A. Combinatorial chemistry and molecular diversity. An overview Journal of Chemical Information and Computer Sciences 37: 1 (JAN- FEB 1997) 134-140 Lemmo, A.V.; Fisher, J.T.; Geysen, H.M.; Rose, D.J. Characterization of an inkjet chemical microdispenser for combinatorial library synthesis Analytical Chemistry 69: 4 (FEB 15 1997) 543-551 Eisinger, D.P.; Trumpower, B.L. Long-inverse PCR to generate regional peptide libraries by codon mutagenesis Biotechniques 22: 2 (FEB 1997) 250 Berlin, K.; Jain, R.K.; Tetzlaff, C.; Steinbeck, C.; Richert, C. Spectrometrically monitored selection experiments: Quantitative laser desorption mass spectrometry of small chemical libraries Chemistry & Biology 4: 1 (JAN 1997) 63-77 Dolezalova, H.; Vojtesek, B.; Kovarik, J. Epitope analysis of the human p53 tumour suppressor protein Folia Biologica 43: 1 (1997) 49-51 Webb, S.R.; Lee, H.; Hall, J.C. Cloning and expression in Escherichia coli of an anti-cyclohexanedione single-chain variable antibody fragment and comparison to the parent monoclonal antibody Journal of Agricultural and Food Chemistry 45: 2 (FEB 1997) 535-541 Krebber, A.; Bornhauser, S.; Burmester, J.; Honegger, A.; Willuda, J.; Bosshard, H.R.; Pluckthun, A. Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system Journal of Immunological Methods 201: 1 (FEB 14 1997) 35-55 Stump, M.D.; MadisonAntenucci, S.; Kokoska, R.J.; Steege, D.A. Filamentous phage IKe mRNAs conserve form and function despite divergence in regulatory elements Journal of Molecular Biology 266: 1 (FEB 14 1997) 51-65 Burgess, K.; Ibarzo, J.; Linthicum, D.S.; Russell, D.H.; Shin, H.; Shitangkoon, A.; Totani, R.; Zhang, A.J. Solid phase syntheses of oligoureas Journal of the American Chemical Society 119: 7 (FEB 19 1997) 1556-1564 Meissner, A.; Bloch, P.; Humpfer, E.; Spraul, M.; Sorensen, O.W. Reduction of inhomogeneous line broadening in two-dimensional high- resolution MAS NMR spectra of molecules attached to swelled resins in solid-phase synthesis Journal of the American Chemical Society 119: 7 (FEB 19 1997) 1787-1788 Versalovic, J.; Lupski, J.R. Missense mutations in the 3' end of the Escherichia coli dnaG gene do not abolish primase activity but do confer the chromosome-segregation- defective (par) phenotype Microbiology - UK 143 (FEB 1997) 585-594 Warren, M.S.; Benkovic, S.J. Combinatorial manipulation of three key active site residues in glycinamide ribonucleotide transformylase Protein Engineering 10: 1 (JAN 1997) 63-68 Gray, N.S.; Kwon, S.; Schultz, P.G. Combinatorial synthesis of 2,9-substituted purines Tetrahedron Letters 38: 7 (FEB 17 1997) 1161-1164 Hoogenboom, H.R. Designing and optimizing library selection strategies for generating high-affinity antibodies Trends in Biotechnology 15: 2 (FEB 1997) 62-70 Marzari, R.; Sblattero, D.; Righi, M.; Bradbury, A. Extending filamentous phage host range by the grafting of a heterologous receptor binding domain Gene 185: 1 (JAN 31 1997) 27-33 Graus, Y.F.; deBaets, M.H.; Parren, P.W.H.I.; BerrihAknin, S.; Wokke, J.; Vriesman, P.J.V.; Burton, D.R. Human anti-nicotinic acetylcholine receptor recombinant Fab fragments isolated from thymus-derived phage display libraries from Myasthenia gravis patients reflect predominant specificities in serum and block the action of pathogenic serum antibodies Journal of Immunology 158: 4 (FEB 15 1997) 1919-1929 Chin, J.; Fell, B.; Shapiro, M.J.; Tomesch, J.; Wareing, J.R.; Bray, A.M. Magic angle spinning NMR for reaction monitoring and structure determination of molecules attached to multipin crowns Journal of Organic Chemistry 62: 3 (FEB 7 1997) 538-539 Webster, R.E. Biology of the filamentous bacteriophage Phage Display of Peptides and Proteins (1996) 1-20 Kay, B.K.; Hoess, R.H. Principles and applications of phage display Phage Display of Peptides and Proteins (1996) 21-34 Armstrong, N.; Adey, N.B.; McConnell, S.J.; Kay, B.K. Vectors for phage display Phage Display of Peptides and Proteins (1996) 35-53 Adey, N.B.; Sparks, A.B.; Beasley, J.; Kay, B.K. Construction of random peptide libraries in bacteriophage M13 Phage Display of Peptides and Proteins (1996) 67-78 Dottavio, D. Phagemid-displayed peptide libraries Phage Display of Peptides and Proteins (1996) 113-125 Malik, P.; Terry, T.D.; Perham, R.N. Multiple display of foreign peptide epitopes on filamentous bacteriophage virions Phage Display of Peptides and Proteins (1996) 127-139 duPlessis, D.H.; Jordaan, P. Phage libraries displaying random peptides derived from a target sequence Phage Display of Peptides and Proteins (1996) 141-150 Suter, M.; Foti, M.; Ackermann, M.; Crameri, R. A cDNA cloning system based on filamentous phage: Selection and enrichment of functional gene products by protein/ligand interactions made possible by linkage of recognition and replication functions Phage Display of Peptides and Proteins (1996) 195-214 Sodoyer, R.; Aujame, L.; Geoffroy, F.; Pion, C.; Peubez, I.; Montegue, B.; Jacquemot, P.; Dubayle, J. Multicombinatorial libraries and combinatorial infection: Practical considerations for vector design Phage Display of Peptides and Proteins (1996) 215-226 Sparks, A.B.; Adey, N.B.; Cwirla, S.; Kay, B.K. Screening phage-displayed random peptide libraries Phage Display of Peptides and Proteins (1996) 227-253 McCafferty, J. Phage display: Factors affecting panning efficiency Phage Display of Peptides and Proteins (1996) 261-276 Janda, K.D.; Lo, L.C.; Lo, C.H.L.; Sim, M.M.; Wang, R.; Wong, C.H.; Lerner, R.A. Chemical selection for catalysis in combinatorial antibody libraries Science 275: 5302 (FEB 14 1997) 945-948 Gopalsamy, A.; Pallai, P.V. Combinatorial synthesis of heterocycles: Solid phase synthesis of 2- arylquinoline-4-carboxylic acid derivatives Tetrahedron Letters 38: 6 (FEB 10 1997) 907-910 Nefzi, A.; Ostresh, J.M.; Meyer, J.P.; Houghten, R.A. Solid phase synthesis of heterocyclic compounds from linear peptides: Cyclic ureas and thioureas Tetrahedron Letters 38: 6 (FEB 10 1997) 931-934 VolkmerEngert, R.; Hoffmann, B.; SchneiderMergener, J. Stable attachment of the HMB-linker to continuous cellulose membranes for parallel solid phase spot synthesis Tetrahedron Letters 38: 6 (FEB 10 1997) 1029-1032 Vagner, J.; Krchnak, V.; Lebl, M.; Barany, G. Solid-phase organic synthesis: Creation of carbon-carbon double bonds under mild conditions by Wittig-type reactions Collection of Czechoslovak Chemical Communications 61: 12 (DEC 1996) 1697-1702 From owner-repertoires@net.bio.net Thu Apr 03 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "Janet Clench, Library, Tel:(39 6)91093220" Newsgroups: bionet.molecules.repertoires Subject: When its spring again we'll bring again ... updates from Pomezia! ciao Date: 4 Apr 1997 02:03:11 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 320 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5i2jbq$cco@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net ******************************************************** SUBJECT: Combinatorial & Phage Libraries DATE: February 24, March 3,10,17 1997 ******************************************************* Finnern, R.; Pedrollo, E.; Fisch, I.; Wieslander, J.; Marks, J.D.; Lockwood, C.M.; Ouwehand, W.H. Human autoimmune anti-proteinase 3 scFv from a phage display library Clinical and Experimental Immunology 107: 2 (FEB 1997) 269-281 Combinatorial chemistry Drug Discovery Today 2: 2 (FEB 1997) 81-81 Eliseev, A.V.; Nelen, M.I. Use of molecular recognition to drive chemical evolution .1. Controlling the composition of an equilibrating mixture of simple arginine receptors Journal of the American Chemical Society 119: 5 (FEB 5 1997) 1147-1148 Harper, K.; Kerschbaumer, R.J.; Ziegler, A.; Macintosh, S.M.; Cowan, G.H.; Himmler, G.; Mayo, M.A.; Torrance, L. A scFv-alkaline phosphatase fusion protein which detects potato leafroll luteovirus in plant extracts by ELISA Journal of Virological Methods 63: 1-2 (JAN 1997) 237-242 Janniere, L.; Bidnenko, V.; McGovern, S.; Ehrlich, S.D. Replication terminus for DNA polymerase I during initiation of pAM beta 1 replication: Role of the plasmid-encoded resolution system Molecular Microbiology 23: 3 (FEB 1997) 525-535 Magliani, W.; Conti, S.; DeBernardis, F.; Gerloni, M.; Bertolotti, D.; Mozzoni, P.; Cassone, A.; Polonelli, L. Therapeutic potential of antiidiotype single chain antibodies with yeast killer toxin activity Nature Biotechnology 15: 2 (FEB 1997) 155-158 Studer, A.; Hadida, S.; Ferritto, R.; Kim, S.Y.; Jeger, P.; Wipf, P.; Curran, D.P. Fluorous synthesis: A fluorous-phase strategy for improving separation efficiency in organic synthesis Science 275: 5301 (FEB 7 1997) 823-826 Lee, B.H. Solid-phase synthesis of cyclooctadepsipeptide PF1022A analogs using a cyclization-cleavage method with oxime resin Tetrahedron Letters 38: 5 (FEB 3 1997) 757-760 Proulx, C.; Chartrand, P.; Roy, V.; Goldman, M.; Decary, F.; Rinfret, A. Human monoclonal Fab fragments recovered from a combinatorial library bind specifically to the platelet HPA-1a alloantigen on glycoprotein IIb-IIIa Vox Sanguinis 72: 1 (1997) 52-60 Uozumi, Y. Preparation of combinatorial library indexed by molecular tags Journal of Synthetic Organic Chemistry Japan 55: 1 (JAN 1997) 65-71 Harada, T.; Katada, J.; Tachiki, A.; Asari, T.; Iijima, K.; Uno, I.; Ojima, I.; Hayashi, Y. Development of the new potent non-peptide gpIIb/IIIa antagonist NSL- 95301 by utilizing combinatorial technique Bioorganic & Medicinal Chemistry Letters 7: 2 (JAN 21 1997) 209-212 Vanwetswinkel, S.; MarchandBrynaert, J.; Fastrez, J. Selection of the most active enzymes from a mixture of phage-displayed beta-lactamase mutants (Vol 6, pg 789, 1996) Bioorganic & Medicinal Chemistry Letters 7: 2 (JAN 21 1997) 239-239 Terrett, N. Combinatorial chemistry Drug Discovery Today 2: 1 (JAN 1997) 37-37 Hennecke, M.; Kola, A.; Baensch, M.; Wrede, A.; Klos, A.; Bautsch, W.; Kohl, J. A selection system to study C5a-C5a-receptor interactions: Phage display of a novel C5a anaphylatoxin, Fos-C5a(Ala27) Gene 184: 2 (JAN 15 1997) 263-272 Marasco, W.A. Intrabodies: Turning the humoral immune system outside in for intracellular immunization Gene Therapy 4: 1 (JAN 1997) 11-15 Davenport, M.P.; Smith, K.J.; Barouch, D.; Reid, S.W.; Bodnar, W.M.; Willis, A.C.; Hunt, D.F.; Hill, A.V.S. HLA class I binding motifs derived from random peptide libraries differ at the COOH terminus from those of eluted peptides Journal of Experimental Medicine 185: 2 (JAN 20 1997) 367-371 Kuebler, P.J.; Nixon, D.F. Cytotoxic T cell induction with ratchet peptide libraries Vaccine 14: 17-18 (DEC 1996) 1664-1670 Downs, G.M.; Barnard, J.M. Techniques for generating descriptive fingerprints in combinatorial libraries Journal of Chemical Information and Computer Sciences 37: 1 (JAN- FEB 1997) 59-61 Warr, W.A. Combinatorial chemistry and molecular diversity. An overview Journal of Chemical Information and Computer Sciences 37: 1 (JAN- FEB 1997) 134-140 Lemmo, A.V.; Fisher, J.T.; Geysen, H.M.; Rose, D.J. Characterization of an inkjet chemical microdispenser for combinatorial library synthesis Analytical Chemistry 69: 4 (FEB 15 1997) 543-551 Eisinger, D.P.; Trumpower, B.L. Long-inverse PCR to generate regional peptide libraries by codon mutagenesis Biotechniques 22: 2 (FEB 1997) 250 Berlin, K.; Jain, R.K.; Tetzlaff, C.; Steinbeck, C.; Richert, C. Spectrometrically monitored selection experiments: Quantitative laser desorption mass spectrometry of small chemical libraries Chemistry & Biology 4: 1 (JAN 1997) 63-77 Dolezalova, H.; Vojtesek, B.; Kovarik, J. Epitope analysis of the human p53 tumour suppressor protein Folia Biologica 43: 1 (1997) 49-51 Webb, S.R.; Lee, H.; Hall, J.C. Cloning and expression in Escherichia coli of an anti-cyclohexanedione single-chain variable antibody fragment and comparison to the parent monoclonal antibody Journal of Agricultural and Food Chemistry 45: 2 (FEB 1997) 535-541 Krebber, A.; Bornhauser, S.; Burmester, J.; Honegger, A.; Willuda, J.; Bosshard, H.R.; Pluckthun, A. Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system Journal of Immunological Methods 201: 1 (FEB 14 1997) 35-55 Stump, M.D.; MadisonAntenucci, S.; Kokoska, R.J.; Steege, D.A. Filamentous phage IKe mRNAs conserve form and function despite divergence in regulatory elements Journal of Molecular Biology 266: 1 (FEB 14 1997) 51-65 Burgess, K.; Ibarzo, J.; Linthicum, D.S.; Russell, D.H.; Shin, H.; Shitangkoon, A.; Totani, R.; Zhang, A.J. Solid phase syntheses of oligoureas Journal of the American Chemical Society 119: 7 (FEB 19 1997) 1556-1564 Meissner, A.; Bloch, P.; Humpfer, E.; Spraul, M.; Sorensen, O.W. Reduction of inhomogeneous line broadening in two-dimensional high- resolution MAS NMR spectra of molecules attached to swelled resins in solid-phase synthesis Journal of the American Chemical Society 119: 7 (FEB 19 1997) 1787-1788 Versalovic, J.; Lupski, J.R. Missense mutations in the 3' end of the Escherichia coli dnaG gene do not abolish primase activity but do confer the chromosome-segregation- defective (par) phenotype Microbiology - UK 143 (FEB 1997) 585-594 Warren, M.S.; Benkovic, S.J. Combinatorial manipulation of three key active site residues in glycinamide ribonucleotide transformylase Protein Engineering 10: 1 (JAN 1997) 63-68 Gray, N.S.; Kwon, S.; Schultz, P.G. Combinatorial synthesis of 2,9-substituted purines Tetrahedron Letters 38: 7 (FEB 17 1997) 1161-1164 Hoogenboom, H.R. Designing and optimizing library selection strategies for generating high-affinity antibodies Trends in Biotechnology 15: 2 (FEB 1997) 62-70 Marzari, R.; Sblattero, D.; Righi, M.; Bradbury, A. Extending filamentous phage host range by the grafting of a heterologous receptor binding domain Gene 185: 1 (JAN 31 1997) 27-33 Graus, Y.F.; deBaets, M.H.; Parren, P.W.H.I.; BerrihAknin, S.; Wokke, J.; Vriesman, P.J.V.; Burton, D.R. Human anti-nicotinic acetylcholine receptor recombinant Fab fragments isolated from thymus-derived phage display libraries from Myasthenia gravis patients reflect predominant specificities in serum and block the action of pathogenic serum antibodies Journal of Immunology 158: 4 (FEB 15 1997) 1919-1929 Chin, J.; Fell, B.; Shapiro, M.J.; Tomesch, J.; Wareing, J.R.; Bray, A.M. Magic angle spinning NMR for reaction monitoring and structure determination of molecules attached to multipin crowns Journal of Organic Chemistry 62: 3 (FEB 7 1997) 538-539 Webster, R.E. Biology of the filamentous bacteriophage Phage Display of Peptides and Proteins (1996) 1-20 Kay, B.K.; Hoess, R.H. Principles and applications of phage display Phage Display of Peptides and Proteins (1996) 21-34 Armstrong, N.; Adey, N.B.; McConnell, S.J.; Kay, B.K. Vectors for phage display Phage Display of Peptides and Proteins (1996) 35-53 Adey, N.B.; Sparks, A.B.; Beasley, J.; Kay, B.K. Construction of random peptide libraries in bacteriophage M13 Phage Display of Peptides and Proteins (1996) 67-78 Dottavio, D. Phagemid-displayed peptide libraries Phage Display of Peptides and Proteins (1996) 113-125 Malik, P.; Terry, T.D.; Perham, R.N. Multiple display of foreign peptide epitopes on filamentous bacteriophage virions Phage Display of Peptides and Proteins (1996) 127-139 duPlessis, D.H.; Jordaan, P. Phage libraries displaying random peptides derived from a target sequence Phage Display of Peptides and Proteins (1996) 141-150 Suter, M.; Foti, M.; Ackermann, M.; Crameri, R. A cDNA cloning system based on filamentous phage: Selection and enrichment of functional gene products by protein/ligand interactions made possible by linkage of recognition and replication functions Phage Display of Peptides and Proteins (1996) 195-214 Sodoyer, R.; Aujame, L.; Geoffroy, F.; Pion, C.; Peubez, I.; Montegue, B.; Jacquemot, P.; Dubayle, J. Multicombinatorial libraries and combinatorial infection: Practical considerations for vector design Phage Display of Peptides and Proteins (1996) 215-226 Sparks, A.B.; Adey, N.B.; Cwirla, S.; Kay, B.K. Screening phage-displayed random peptide libraries Phage Display of Peptides and Proteins (1996) 227-253 McCafferty, J. Phage display: Factors affecting panning efficiency Phage Display of Peptides and Proteins (1996) 261-276 Janda, K.D.; Lo, L.C.; Lo, C.H.L.; Sim, M.M.; Wang, R.; Wong, C.H.; Lerner, R.A. Chemical selection for catalysis in combinatorial antibody libraries Science 275: 5302 (FEB 14 1997) 945-948 Gopalsamy, A.; Pallai, P.V. Combinatorial synthesis of heterocycles: Solid phase synthesis of 2- arylquinoline-4-carboxylic acid derivatives Tetrahedron Letters 38: 6 (FEB 10 1997) 907-910 Nefzi, A.; Ostresh, J.M.; Meyer, J.P.; Houghten, R.A. Solid phase synthesis of heterocyclic compounds from linear peptides: Cyclic ureas and thioureas Tetrahedron Letters 38: 6 (FEB 10 1997) 931-934 VolkmerEngert, R.; Hoffmann, B.; SchneiderMergener, J. Stable attachment of the HMB-linker to continuous cellulose membranes for parallel solid phase spot synthesis Tetrahedron Letters 38: 6 (FEB 10 1997) 1029-1032 Vagner, J.; Krchnak, V.; Lebl, M.; Barany, G. Solid-phase organic synthesis: Creation of carbon-carbon double bonds under mild conditions by Wittig-type reactions Collection of Czechoslovak Chemical Communications 61: 12 (DEC 1996) 1697-1702 From owner-repertoires@net.bio.net Thu Apr 03 23:00:00 1997 Path: biosci!biosci!not-for-mail From: jauliac sebastien Newsgroups: bionet.molecules.repertoires Subject: wortmaninn Date: 4 Apr 1997 05:09:27 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 4 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <333EACA3.46F9@micronet.fr> Reply-To: somyos@micronet.fr NNTP-Posting-Host: net.bio.net i would like to know if all the penicillium can produce wortmannin From owner-repertoires@net.bio.net Sat Apr 12 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Brian Scott Newsgroups: bionet.molecules.repertoires Subject: Free molecular visualization workshops for profs Date: 13 Apr 1997 03:42:34 -0700 Organization: University of Massachusetts Lines: 56 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <334C39D2.1755@biochem.umass.edu> NNTP-Posting-Host: net.bio.net Free Workshops on Molecular Visualization for Undergraduate Bioscience Teaching Amherst MA -- Summer, 1997-8 Understanding the three-dimensional structures of proteins, DNA, RNA, and their interactions is difficult from flat pictures, yet grasping structure is important to understanding function. Free software is now available which displays attention-grabbing 3D animated images of biological molecules in depth-cued spacefilling color. RasMol (http://www.umass.edu/microbio/rasmol), which works on Windows or Macs, encourages self-directed exploration. Chime (http://www.umass.edu/microbio/chime) allows annotated preset views of molecules to be delivered as web tutorials. Free, hands-on workshops will be held this summer at the University of Massachusetts in Amherst to prepare college faculty with no prior experience to use molecular visualization in their classes. Participants will travel to the Amherst campus on three separate days (two this summer, one the following summer), and are responsible for their own travel expenses (and therefore are expected primarily from the Northeastern USA). Overnight accomodations will be provided free to those needing them (based on distance traveled). Participants will be expected to incorporate molecular visualization into their teaching, and to mentor two faculty colleagues at their home institution. The workshops will be led by Eric Martz, a Professor in the Department of Microbiology. Martz has innovated molecular structure tutorials which are in use throughout the USA and in dozens of other countries. The web site he created (see above) was visited by 40,000 people in 1996. 1997 dates are June 17 and 30 (workshop A), or June 19 and July 2 (workshop B). Workshops A and B also meet for one day in late June 1998. To obtain more detailed information and a REGISTRATION FORM, visit http://www.umass.edu/microbio/rasmol/workshop.htm or FAX a request to 413-545-1578, or email a request to emartz@microbio.umass.edu. Supported by the National Science Foundation (Undergraduate Faculty Enhancement, Division of Undergraduate Education) and the University of Massachusetts. /* - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Eric Martz, Professor (Immunology), Dept Microbiology, Univ Massachusetts, Amherst MA US 01003-5720 413-545-2325 FAX:545-1578. RasMol Home Page, http://www.umass.edu/microbio/rasmol Other web projects listed at http://www.umass.edu/microbio/rasmol/em-web.htm - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -*/ From owner-repertoires@net.bio.net Sat Apr 12 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Jan Engberg Newsgroups: bionet.molecules.repertoires Subject: Postdoc positions Date: 13 Apr 1997 03:39:15 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 65 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Two post-doc positions in phage-display technology. I am seeking individuals at the post-doctoral level with a strong background in molecular genetics and some experience with protein expression and purification. Both positions are available from September/October of 1997 and will run for three years. The work on both positions makes use of phage display technlogy and a close collaboration between the two post-doctoral fellows is expected. Job descriptions: 1) "Generation of recombinant antibody-toxins directed against tumor associated peptide/HLA complexes with T cell receptor-like specificity" Expression of specific peptides in complex with MHC molecules has been shown to be associated with cancer. For example, human melanomas express tumor-specific peptides that are presented to the immune system in complex with class I HLA-A2 molecules. Specific targeting of drugs to these cells by using these specific peptide/HLA complexes will be a useful and promising approach. In a model system, we have recently generated a recombinant antibody-toxin fusion protein targeted to a peptide/MHC with T cell receptor-like specificity and demonstrated that killing of antigen-presenting cells occured in a peptide specific maner(*). The results suggest that such an approach is feasible to apply to novel immunotherapeutic strategies against human cancer. * Andersen, P. S. et al (1996) PNAS 93(3), 1820-1825. * Stryhn, A. et al (1996) PNAS 93, 10338-10342. * Reiter, Y. et al (1997) PNAS April issue. 2) "Novel approaches in the study of differential gene expression by developing the phage display technology" I have been funded together with prof. Brian F. C. Clark , University of Aarhus, to develop the technology of displaying antibody fragments on filamentous phage in a way that allows us to obtain antibodies directed against gene products that are expressed differentially in human epidermal keratinocytes upon differentiation in vitro in cell cultures. Such gene expression will reflect the cellular process of differentiation. Thus with a panel of antibodies against the corresponding proteins it will be posible to gain information on the differentiation of the keratinocytes. We have recently developed a method with potential for such analysis(*) but a larger antibody library is necessary, since the method requires one single source library from which antibodies with high affinities can be selected against a broad variety of antigens. Therefore, one primery goal is to generate a large human naive library of antibody fragments displayed on filamentous phage. * Stausbl-Grn, B. et al (1996) FEBS letters 391, 71-75. Further information may be obtained from professor Jan Engberg. Please address - BEFORE THE 1st OF JUNE - applications, which should contain a full CV, a short summary of research experience and the full postal and e-mail addresses and fax and phone numbers of three references to: Professor Jan Engberg, The Royal Danish School of Pharmacy, Department of Biological Sciences, Universitetsparken 2, DK-2100 Copenhagen, Denmark. Phone: +45 35 37 08 50 ext 384. Direct: +45 35 37 66 67 await tone, then press 384 Fax: +45 35 37 57 92. e-mail: JE@CHARON.DFH.DK From owner-repertoires@net.bio.net Sun Apr 13 23:00:00 1997 Path: biosci!biosci!not-for-mail From: hho@lpat.azm.nl (H. Hoogenboom) Newsgroups: bionet.molecules.repertoires Subject: Jobs in Maastricht Date: 14 Apr 1997 11:18:10 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 74 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5itrbt$jbc@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net The Antibody Engineering group CESAME, based in the Department of Pathology, University Hospital Maastricht, the Netherlands, has available positions for : Three Research Scientists Antibody Design and Engineering You will be involved in network research projects financed by the European Community as part of the 4th Biotechnology Framework. The research in Maastricht focuses on the development of strategies for the immunotherapy of cancer : (1) Recombinant bispecific antibodies for the treatment of B-cell non-Hodgkin's disease. This project involves the design and construction of 'diabodies', antibody fragments with two binding sites, using phage display and expression in bacteria (McGuinness et al. Nat Biotechnol 14, 1149-54, 1996). These proteins will be tested for their in vitro en in vivo T-cell-retargeting capacity. (2) MHC-peptide complex specific antibodies for tumor targeting. The candidate will study the possibility to derive human antibodies specific for recombinant MHC-Class I molecules complexed to tumor-associated peptides (similar to Andersen et al. Proc Natl Acad Sci USA 93, 1820-24, 1996, for class II). The tumor-targeting efficacy of these antibodies will be tested in close collaboration with other partners of the programme. (3) Intracellular antibodies : The candidate will be involved in designing new antibody scaffolds, creating synthetic phage antibody libraries and selecting high affinity ligands to certain tumor-associated intracellular targets (Persic et al Gene 187:1-16, 1997). You will be based in the Department of Pathology, University Hospital Maastricht, in the Netherlands, working in the multidisciplinary research team CESAME, in which antibody engineering en phage display are being used as key technologies to derive useful immunotargeting molecules (Hoogenboom, TIBTECH 15, 62-70; 1997). Applicants have a PhD in a relevant areas (molecular immunology, protein engineering, oncology) or equivalent experience; additional postdoctoral experience abroad is a major advantage. Expertise in molecular biology and familiarity with protein biochemistry are essential. Salary is according to age and experience within band 10 (to max Dfl. 6.092,-). Appointments are full time, for 2-3 years, and available within the next 4 months. More information about these positions may be obtained from dr. ir. H.R.J.M. Hoogenboom, tel. 31-43-3874630, or via E-mail : hho@lpat.azm.nl Please send, before the 1st of May, (1) Curriculum Vitae including details of two referees, (2) a 1-page description of research interests, and (3) a motivated ranking between the three cited projects, to dr. ir. H.R.J.M. Hoogenboom, Department of Pathology, Academisch Ziekenhuis Maastricht, P.O. Box 5800, 6202 AZ Maastricht, the Netherlands; or FAX 31-43-3876613. ******************************** Hennie R.J.M. Hoogenboom CESAME at Department Pathology University Hospital Maastricht P. Debyelaan 25 P.O. Box 5800 6202 AZ MAASTRICHT THE NETHERLANDS tel. 00-31-43-387.4630 00-31-43-387.6612 FAX 00-31-43-387.6613 E-mail HHO@LPAT.AZM.NL ******************************** From owner-repertoires@net.bio.net Wed Apr 16 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "Janet Clench, Library, Tel:(39 6)91093220" Newsgroups: bionet.molecules.repertoires Subject: here come the refs, here come the refs, everybody say now here come Date: 17 Apr 1997 04:06:01 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 221 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5j4u0h$e9p@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net ******************************************************* SUBJECT: Combinatorial & Phage Libraries DATE: March 24,31 April 7 1997 ****************************************************** Schullek, J.R.; Butler, J.H.; Ni, Z.J.; Chen, D.; Yuan, Z.Y. A high-density screening format for encoded combinatorial libraries: Assay miniaturization and its application to enzymatic reactions Analytical Biochemistry 246: 1 (MAR 1 1997) 20-29 Sollazzo, M.; Venturini, S.; Lorenzetti, S.; Pinola, M.; Martin, F. Engineering minibody-like ligands by design and selection Antibody Engineering (Series: Chemical Immunology) 65 (1997) 1-17 Parren, P.W.H.I.; Burton, D.R. Antibodies against HIV-1 from phage display libraries: Mapping of an immune response and progress towards antiviral immunotherapy Antibody Engineering (Series: Chemical Immunology) 65 (1997) 18-56 Yang, Y.Y.; Fischer, P.; Leu, S.J.C.; Olee, T.; Carson, D.A.; Chen, P.P. IgG rheumatoid factors isolated by the surface-displaying phage library technique Immunogenetics 45: 5 (1997) 301-310 Suck, D. Common fold, common function, common origin? Nature Structural Biology 4: 3 (MAR 1997) 161-165 Terskikh, A.V.; LeDoussal, J.M.; Crameri, R.; Fisch, I.; Mach, J.P.; Kajava, A.V. ''Peptabody'': A new type of high avidity binding protein Proceedings of the National Academy of Sciences of the United States of America 94: 5 (MAR 4 1997) 1663-1668 Welschof, M.; Terness, P.; Kipriyanov, S.M.; Stanescu, D.; Breitling, F.; Dorsam, H.; Dubel, S.; Little, M.; Opelz, G. The antigen-binding domain of a human IgG-anti-F(ab')(2) autoantibody Proceedings of the National Academy of Sciences of the United States of America 94: 5 (MAR 4 1997) 1902-1907 Gaynor, B.; Putterman, C.; Valadon, P.; Spatz, L.; Scharff, M.D.; Diamond, B. Peptide inhibition of glomerular deposition of an anti-DNA antibody Proceedings of the National Academy of Sciences of the United States of America 94: 5 (MAR 4 1997) 1955-1960 Loo, J.A.; Hu, P.F.; McConnell, P.; Mueller, W.T.; Sawyer, T.K.; Thanabal, V. A study of Src SH2 domain protein-phosphopeptide binding interactions by electrospray ionization mass spectrometry Journal of the American Society for Mass Spectrometry 8: 3 (MAR 1997) 234-243 Buchli, P.J.; Wu, Z.N.; Ciardelli, T.L. The functional display of interleukin-2 on filamentous phage Archives of Biochemistry and Biophysics 339: 1 (MAR 1 1997) 79-84 Thiesen, H.J. >From designer zinc finger domains by phage display to transcription response modifiers Immunology Methods Manual - The Comprehensive Sourcebook of Techniques, Vol 1 (1997) 365-373 Hammer, J.; Sinigaglia, F. Analysis of MHC class II binding motifs with bacteriophage peptide display libraries Immunology Methods Manual - The Comprehensive Sourcebook of Techniques, Vol 2 (1997) 625-635 Pinilla, C.; Appel, J.R.; Houghten, R.A. The generation of peptide combinatorial libraries using tea-bag synthesis: Identification of B-cell epitopes Immunology Methods Manual - The Comprehensive Sourcebook of Techniques, Vol 2 (1997) 835-845 vanderHeijden, J.; deKruif, J.; deBruin, T.W.A.; Logtenberg, T. Analysis of the humoral immune response using combinatorial phage display libraries Immunology Methods Manual - The Comprehensive Sourcebook of Techniques, Vol 2 (1997) 1095-1102 Kaur, K.J.; Khurana, S.; Salunke, D.M. Topological analysis of the functional mimicry between a peptide and a carbohydrate moiety Journal of Biological Chemistry 272: 9 (FEB 28 1997) 5539-5543 Jelinek, R.; Terry, T.D.; Gesell, J.J.; Malik, P.; Perham, R.N.; Opella, S.J. NMR structure of the principal neutralizing determinant of HIV-1 displayed in filamentous bacteriophage coat protein Journal of Molecular Biology 266: 4 (MAR 7 1997) 649-655 Shibata, N.; Baldwin, J.E.; Wood, M.E. Resin-bound peptide libraries showing specific metal ion binding Bioorganic & Medicinal Chemistry Letters 7: 4 (FEB 18 1997) 413-416 Boger, D.L.; Chai, W.Y.; Ozer, R.S.; Andersson, C.M. Solution-phase combinatorial synthesis via the olefin metathesis reaction Bioorganic & Medicinal Chemistry Letters 7: 4 (FEB 18 1997) 463-468 Krantz, A. Red cell-mediated therapy: Opportunities and challenges Blood Cells Molecules and Diseases 23: 3 (FEB 15 1997) 58-68 Egner, B.J.; Bradley, M. Analytical techniques for solid-phase organic and combinatorial synthesis Drug Discovery Today 2: 3 (MAR 1997) 102-109 Terrett, N. Combinatorial chemistry - Anticancer agents from OBOC libraries Drug Discovery Today 2: 3 (MAR 1997) 124-124 Lobel, L.I.; Rausch, P.; Trakht, I.; Pollak, S.; Lustbader, J.W. Filamentous phage displaying the extracellular domain of the hLH/CG receptor bind hCG specifically Endocrinology 138: 3 (MAR 1997) 1232-1239 Stern, B.; Denisova, G.; Buyaner, D.; Raviv, D.; Gershoni, J.M. Helical epitopes determined by low-stringency antibody screening of a combinatorial peptide library FASEB Journal 11: 2 (FEB 1997) 147-153 Desmet, J.; Wilson, I.A.; Joniau, M.; DeMaeyer, M.; Lasters, I. Computation of the binding of fully flexible peptides to proteins with flexible side chains FASEB Journal 11: 2 (FEB 1997) 164-172 DeJaeger, G.; Buys, E.; Eeckhout, D.; Bruyns, A.M.; DeNeve, M.; DeWilde, C.; Gerats, T.; VanMontagu, M.; Fischer, R.; Depicker, A. Use of phage display for isolation and characterization of single-chain variable fragments against dihydroflavonol 4-reductase from Petunia hybrida FEBS Letters 403: 2 (FEB 17 1997) 116-122 Obrecht, D.; Abrecht, C.; Grieder, A.; Villalgordo, J.M. A novel and efficient approach for the combinatorial synthesis of structurally diverse pyrimidines on solid support Helvetica Chimica Acta 80: 1 (1997) 65-72 Gershoni, J.M.; Stern, B.; Denisova, G. Combinatorial libraries, epitope structure and the prediction of protein conformations Immunology Today 18: 3 (MAR 1997) 108-110 Malik, P.; Perham, R.N. Simultaneous display of different peptides on the surface of filamentous bacteriophage Nucleic Acids Research 25: 4 (FEB 15 1997) 915-916 Gebhardt, K.; Lauvrak, V.; Babaie, E.; Eijsink, V.; Lindqvist, B.H. Adhesive peptides selected by phage display: Characterization, applications and similarities with fibrinogen Peptide Research 9: 6 (NOV-DEC 1996) 269-278 Holliger, P.; Riechmann, L. A conserved infection pathway for filamentous bacteriophages is suggested by the structure of the membrane penetration domain of the minor coat protein g3p from phage fd Structure 5: 2 (FEB 15 1997) 265-275 Cheng, Y.; Chapman, K.T. Solid phase synthesis of spiroindoline Tetrahedron Letters 38: 9 (MAR 3 1997) 1497-1500 Shapiro, M.J.; Chin, J.; Marti, R.E.; Jarosinski, M.A. Enhanced resolution in MAS NMR for combinatorial chemistry Tetrahedron Letters 38: 8 (FEB 24 1997) 1333-1336 Spencer, J.V.; Trus, B.L.; Booy, F.P.; Steven, A.C.; Newcomb, W.W.; Brown, J.C. Structure of the herpes simplex virus capsid: Peptide A862-H880 of the major capsid protein is displayed on the rim of the capsomer protrusions Virology 228: 2 (FEB 17 1997) 229-235 Bunin, B.A.; Plunkett, M.J.; Ellman, J.A.; Bray, A.M. The synthesis of a 1680 member 1,4-benzodiazepine library New Journal of Chemistry 21: 1 (JAN 1997) 125-130 From owner-repertoires@net.bio.net Wed Apr 16 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Peter Wang Newsgroups: bionet.molecules.repertoires Subject: VCSM13 helper phage: origin? Date: 17 Apr 1997 03:04:02 -0700 Organization: University of Cambridge, England Lines: 17 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5j4sii$6me@net.bio.net> NNTP-Posting-Host: net.bio.net Does anyone know anything about the origin of the filamentous helper phage VCSM13? It is derived from M13KO7 and is sold by Stratagene. My curiosity was piqued because their Technical Service department did not know what differences there were from M13KO7 nor were they willing to tell me who the original researcher was. Thanks for any insights! --------------------------------------------------------- Peter Wang, M.D., Ph.D. MRC Centre for Protein Engineering, Hills Road, Cambridge, CB2 2QH, England Tel (01223) 402104 (international calls +44-1223-402104) Fax (01223) 402140 ( " " +44-1223-402140) --------------------------------------------------------- From owner-repertoires@net.bio.net Thu Apr 17 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Arpi Heghinian Newsgroups: bionet.molecules.repertoires Subject: IBC's 2nd Annual Conference on Display Technologies Date: 18 Apr 1997 02:06:02 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 39 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <9703178613.AA861309548@ultra-gw.ibcusa.com> NNTP-Posting-Host: net.bio.net Announcing IBC's 2nd Annual Conference on Display Technologies: Advances in Protein Engineering, Drug/Vaccine Development and Molecular Evolution; August 4-5, 1997-Boston, MA TOPICS TO BE DISCUSSED INCLUDE... Display Technologies in Protein and Peptide Optimization Monovalent Phage Display of Proteins New Adenovirus Expression Systems Novel Peptide Pseudoligands Protein-Protein Binding Interactions Phage Display of Small Molecules Breakthroughs in Alternate Display In Vitro Evolution E. Coli Display Combinatorial Directed Evolution Technologies Interactive Mutagenesis and Selection Ribosome Display Display Libraries and Drug Discovery Genomic Mosaic Phage Library Construction Receptor Oligomerization Target Discovery Random Peptide Libraries Qualitative Apects of Phage Display AND MORE!!! For More Information Call IBC USA Conferences at 508-481-6400, Fax: 508-481-4473 or email aheghinian@ibcusa.com for complete conference brochure or Register Online at http://www.ibcusa.com/conf/display From owner-repertoires@net.bio.net Sun Apr 20 23:00:00 1997 Path: biosci!biosci!not-for-mail From: fleurs@med.unc.edu Newsgroups: bionet.molecules.repertoires Subject: Carolina Workshop on Combinatorial Molecules Date: 21 Apr 1997 01:24:22 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 51 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <199704190059.UAA26592@raven.med.unc.edu> NNTP-Posting-Host: net.bio.net Carolina Workshop on Combinatorial Molecules June 22 - 30, 1997 Theory and Applications of Combinatorial Libraries for Molecular Recognition Studies and Identification of Antagonists Course content: This course will cover the theory and applications of three different types of combinatorial molecules. Students are invited to bring their own target molecules to screen these libraries. Students will learn to work with phage-displayed random peptide libraries, RNA libraries, and combinatorial chemical libraries. First, students will learn how to screen billions of M13 bacteriophage, each displaying different peptides sequences, for the purpose of studying protein-protein interactions. Second, students will learn how to generate and screen combinatorial RNA libraries to discover RNA ligands and antagonists. Third, students will learn how to screen a combinatorial peptide library on and off beads for the purpose of finding chemical agonists and antagonists. This is a hands-on workshop, accompanied by extensive protocols. Instructors: Brian Kay, Department of Biology, University of North Carolina at Chapel Hill, Diversity Sciences Research Institute Mario Geysen, GlaxoWellcome Research Institute, Diversity Sciences Research Institute Jack Keene, Department Of Microbiology, Duke University Medical Center, Diversity Sciences Research Institiute Tuition: $1200. Full consideration will be given to applications received by May 9, 1997. To Apply: Send a brief letter describing how this workshop will further your research project(s), along with your CV to the workshop coordinator: Wayne Litaker Program in Molecular Biology and Biotechnology University of North Carolina at Chapel Hill 442 Taylor Hall, CB # 7100 Chapel Hill, NC 27599-7100 office: 919-929-0067 Fax: 919-966-6821 email: litaker@med.unc.edu For additional information contact: briankay@email.unc.edu OR http://fordham25.bio.unc.edu/workshop.html From owner-repertoires@net.bio.net Mon Apr 21 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: UK PhD studentship Date: 22 Apr 1997 03:47:13 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 48 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5ji46j$an7@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net The Queen's University of Belfast School of Biology and Biochemistry Postgraduate Research (CAST) Studentships 1997-98 A postgraduate studentship under the Co-Operative Awards in Science and Technology (CAST) scheme is available in the following area commencing October 1997: *************************************************** Artificial carbon-phosphorous bond cleaving enzymes for degradation of organophosphates. *************************************************** The project will make use of combinatorial library methodology to select carbon-phosphorous bond cleaving catalytic antibodies from a phage-displayed antibody library and will be carried out in the laboratory of Dr. Andrew Wallace, School of Biology and Biochemistry. Applications are invited from UK and EU candidates who have or expect to obtain a relevant First or Upper Second Class Honours Degree in Chemistry, Biochemistry, Molecular Biology, Biological/Biomedical Sciences or equivalent qualification. This award is sponsored by the Environment and Heritage Service of the Department of the Environment (NI) and is made available in partnership with the Department of Education (NI). Further details and application forms are available from The Director, School of Biology and Biochemistry, The Queen's University of Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL Fax +44-1232-236505 Email sobb.office@qub.ac.uk OR a.wallace@qub.ac.uk. All applications for admission must be received by the Admissions Office, The Queen's University of Belfast no later than 15 JULY 1997. The first allocation of studentships to outstanding applicants will be made in MAY so candidates are encouraged to apply early. The University is a charity established in 1845 to advance education. ------------------------------------------------------------------ Andrew Wallace, Ph.D, Queens University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://web.qub.ac.uk/bb/awpage/wallace.html From owner-repertoires@net.bio.net Mon Apr 21 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: UK PhD studentship cancer research Date: 22 Apr 1997 04:41:18 -0700 Organization: Queens University Belfast Lines: 43 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5ji7ar$frr@mserv1.dl.ac.uk> Reply-To: a.wallace@Queens-Belfast.ac.uk NNTP-Posting-Host: net.bio.net The Queen's University of Belfast Action Cancer PhD Studentship Applications are invited for a three year PhD Studentship funded by Action Cancer to be carried out at Queen's University commencing 1st October 1997. The project is concerned with the application of phage display libraries to the discovery of novel tumour necrosis factor-alpha receptor antagonists. This project will offer training in the following regimes: molecular biology, peptide chemistry and cell biology. Applicants will have, or anticipate by summer '97, a first class or upper second class honours degree or equivalent in an appropriate chemical, biological or biomedical subject. An aptitude for research as indicated by referees' reports is essential. The successful candidate fulfilling the eligibility criteria will recieve a stipend of #6,731 p.a. in addition to payment of full time University fees. The closing date for receipt of applications, which must be supported by two referees' reports, is 1 June 1997. Applications and referees' reports should be sent to Dr M.I.Halliday, Department of Surgery, The Queen's University of Belfast, BT12 6BJ, N. Ireland. Dr M.I.Halliday may be contacted for further information concerning the post by either mail to the above address, e-mail (i.halliday@ qub.ac.uk) or telephone (44-(0)1232-322276). The Queen's University of Belfast is an equal opportunity employer. -- ================================================================== Andrew Wallace,Ph.D., Queens University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://web.qub.ac.uk/bb/awpage/wallace.html ================================================================== From owner-repertoires@net.bio.net Sun Apr 27 23:00:00 1997 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.molecules.repertoires Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 28 Apr 1997 02:48:49 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 242 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <199704280900.CAA11439@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. 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For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. 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You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-repertoires@net.bio.net Sun Apr 27 23:00:00 1997 Path: biosci!biosci!not-for-mail From: gerald.boehm@biochemtech.uni-halle.de (Gerald Boehm) Newsgroups: bionet.molecules.repertoires Subject: Company for triplet oligo synthesis Date: 28 Apr 1997 01:06:34 -0700 Organization: Institut fuer Biotechnologie, Uni Halle Lines: 38 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5k1l6n$b1i@mlucom4.urz.uni-halle.de> NNTP-Posting-Host: net.bio.net Dear Netters, I am looking for a company that can perform triplet oligo synthesis. I need that for "defined randomization" experiments within a phage display project. Is anyone aware of such a company (apart from MorphoSys whose conditions are inacceptable)? "triplet oligo synthesis" means that one uses codons instead of single nucleic acid bases for the synthesis, i.e. you do not have 4 cups with bases but 61 (or 64, if you add stop codons) cups with triplets for the synthesis. Any help would be appreciated. Sincerely Gerald Boehm gerald.boehm@biochemtech.uni-halle.de ---------------------------------------------------------- Gerald Boehm Institut fuer Biotechnologie Martin-Luther-Universitaet Halle-Wittenberg Kurt-Mothes-Strasse 3 D-06120 Halle (Saale) Germany FAX: +345 55 27013 Phone: +345 55 24948 E-Mail: gerald.boehm@biochemtech.uni-halle.de Internet: http://www.biochemtech.uni-halle.de/proteintech ---------------------------------------------------------- From owner-repertoires@net.bio.net Mon Apr 28 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: List of meetings and courses Date: 29 Apr 1997 02:20:23 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 93 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5k4e3d$rju@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net I have put together a list of meetings and courses on phage display and combinatorial libraries, based on information posted to the molreps group over the last few months. Let me know which others there are and please post the details to molreps@dl.ac.uk Andrew Meetings and courses on combinatorial libraries and phage display. ***************************************************************** Cambridge Healthtech Institute Fourth Annual HIGH-THROUGHPUT SCREENING June 25-27 Arlington, Virginia Cambridge Healthtech Institute 1037 Chestnut Street Newton Upper Falls, MA 02164 USA Phone: 617-630-1300 Fax: 617-630-1325 e-mail: chi@healthtech.com http://www.healthtech.com/conferences/ ___________________________________________________ Cambridge Healthtech Institutes First European Conference on Combinatorial Chemistry and High Throughput Screening December 4-5, 1997 Hotel Arts Barcelona Barcelona, Spain Contact: Irene Phelan, Conference Director Phone: 617-630-1362 Fax: 617-630-1325 e-mail: iphelan@healthtech.com Organized by Cambridge Healthtech Institute 1037 Chestnut Steet, Newton Upper Falls, MA 02164 USA http://www/healthtech.com/conferences/ ___________________________________________________ Announcing IBC's 2nd Annual Conference on Display Technologies: Advances in Protein Engineering, Drug/Vaccine Development and Molecular Evolution; August 4-5, 1997-Boston, MA For More Information: Call IBC USA Conferences at 508-481-6400, Fax: 508-481-4473 or email aheghinian@ibcusa.com for complete conference brochure or Register Online at http://www.ibcusa.com/conf/display ___________________________________________________ Carolina Workshop on Combinatorial Molecules June 22 - 30, 1997 Theory and Applications of Combinatorial Libraries for Molecular Recognition Studies and Identification of Antagonists Tuition: $1200. Full consideration will be given to applications received by May 9, 1997. To Apply: Send a brief letter describing how this workshop will further your research project(s), along with your CV to the workshop coordinator: Wayne Litaker Program in Molecular Biology and Biotechnology University of North Carolina at Chapel Hill 442 Taylor Hall, CB # 7100 Chapel Hill, NC 27599-7100 office: 919-929-0067 Fax: 919-966-6821 email: litaker@med.unc.edu For additional information contact: briankay@email.unc.edu OR http://fordham25.bio.unc.edu/workshop.html _____________________________________________________________ Phage Display of Proteins and Peptides Biochemical Society Meeting University College Galway 3-5 September 1997 Deadline for poster registration 6 June 1997 Further information from: Michelle Mandale Meetings Office The Biochemical Society 59 Portland Place London W1N 3AJ Fax: +44 171 637 7626 email: meetings@biochemsoc.org.uk _____________________________________________________________ ------------------------------------------------------------------ Andrew Wallace, Ph.D, Queens University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://web.qub.ac.uk/bb/awpage/wallace.html From owner-repertoires@net.bio.net Tue Apr 29 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Terry Hart Newsgroups: bionet.molecules.repertoires Subject: Protease Conference 20th May 1997 at Smithkline Beecham Date: 30 Apr 1997 07:52:04 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 27 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5k7lpu$en0@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net Just to let you know that The Royal Society of Chemistry will be holding an extremely interesting one day conference on Recent Advances and Future Challenges in Protease Inhibitors and Drug Design on May 20th 1997 at the new SmithKline Beecham Laboratories at Harlow, UK. The Programme comprises: 1. Proteinase Superfamilies and Drug Design (Tom Blundell, Cambridge Univ) 2. Aspartic Proteinases and their inhibitors; from cell to sell (John Kay, UWIST) 3. Matrix metalloprotease inhibitors in Arthritis Therapy- Geoff Lawton (Roche) 4. Metalloprotease Inhibitors (Andrew Faller, SmithKline Beecham) 5. Herpes Protease Inhibition (Richard Jarvest, SmithKline Beecham) 6. Hepatitis C NS3 Protease Inhibitors (T. Wilkinson (Roche) 7. Caspase Inhibition as a therapeutic strategy (Winnie Wong,BASF Bioresearch) Registration costs =75 pounds (U$124) for RSC members and =98 pounds (U$162) Contact Elaine Wellingham (E-mail: confsec@dial.pipex.com) (Fax: +44-1275 853311) for further details and accomodation information. Numbers are limited so if you do not want to miss out, then you will need to book now. From owner-repertoires@net.bio.net Tue Apr 29 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: book on intracellular antibodies Date: 30 Apr 1997 06:25:33 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5k79us$ssj@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net I have received some details about a forthcoming book on intracellular antibodies, edited by Antonino Cattaneo and Silvia Biocca. I will post more details from the publisher when these become available. Andrew > A.Cattaneo and S.Biocca > Intracellular antibodies: developments and > applications. > > The book contains three contributed chapters (out of a total of > eleven), by Andrew Bradbury (on phage display), > Wayne Marasco (on applications to HIV) and Eugenio > Benvenuto (on plantibodies). This is not a > protocol book, but describes concepts, principles, > background information, problems and wild > speculations for future perspectives and applications. > ------------------------------------------------------------------ Andrew Wallace, Ph.D, Queens University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://web.qub.ac.uk/bb/awpage/wallace.html From owner-repertoires@net.bio.net Wed Apr 30 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Action Cancer Research Studentship Date: 1 May 1997 02:52:23 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 46 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5k9nrn$rn5@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net The Queen's University of Belfast Action Cancer PhD Studentship Applications are invited for a three year PhD Studentship funded by Action Cancer to be carried out at Queen's University commencing 1st October 1997. The project is concerned with the application of phage display libraries to the discovery of novel tumour necrosis factor-alpha receptor antagonists. This project will offer training in the following regimes: molecular biology, peptide chemistry and cell biology. Applicants will have, or anticipate by summer '97, a first class or upper second class honours degree or equivalent in an appropriate chemical, biological or biomedical subject. An aptitude for research as indicated by referees' reports is essential. The successful candidate fulfilling the eligibility criteria will receive a stipend 6,731 (pounds Sterling per annum in addition to payment of full-time University fees. The closing date for receipt of applications, which must be supported by two referees' reports, is 1 June 1997. To apply, send a letter of application together with a CV and two referees' reports to Dr M.I.Halliday, Department of Surgery, The Queen's University of Belfast, BT12 6BJ, N. Ireland. Dr M.I.Halliday may be contacted for further information concerning the post by either mail to the above address, e-mail (i.halliday@qub.ac.uk) or telephone (+44-(0)1232-322276). Further details and links to other Queen's University pages are viewable from the web page: http://www.qub.ac.uk/bb/studshps.html The Queen's University of Belfast is an equal opportunity employer. ------------------------------------------------------------------ Andrew Wallace, Ph.D, Queens University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://web.qub.ac.uk/bb/awpage/wallace.html From owner-repertoires@net.bio.net Wed Apr 30 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: UK PhD studentship in catalytic antibodies Date: 1 May 1997 02:16:08 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 52 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5k9jkk$ma6@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net The Queen's University of Belfast School of Biology and Biochemistry Postgraduate Research (CAST) Studentships 1997-98 A postgraduate studentship under the Co-Operative Awards in Science and Technology (CAST) scheme is available in the following area for three years commencing October 1997: *************************************************** Artificial carbon-phosphorous bond cleaving enzymes for degradation of organophosphates. *************************************************** The project will make use of combinatorial library methodology to select carbon-phosphorous bond cleaving catalytic antibodies from a phage-displayed antibody library and will be carried out in the laboratory of Dr. Andrew Wallace, School of Biology and Biochemistry. Applications are invited from UK and EU candidates who have or expect to obtain a relevant First or Upper Second Class Honours Degree in Chemistry, Biochemistry, Molecular Biology, Biological/Biomedical Sciences or equivalent qualification. This award is sponsored by the Environment and Heritage Service of the Department of the Environment (NI) and is made available in partnership with the Department of Education (NI). Further details and application forms are available from The Director, School of Biology and Biochemistry, The Queen's University of Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL Fax +44-1232-236505 Email sobb.office@qub.ac.uk OR a.wallace@qub.ac.uk. Downloadable application forms and links to other Queen's University pages are available from the web page: http://www.qub.ac.uk/bb/studshps.html All applications for admission must be received by the Admissions Office, The Queen's University of Belfast no later than 15 JULY 1997. The first allocation of studentships to outstanding applicants will be made in MAY so candidates are encouraged to apply early. The University is a charity established in 1845 to advance education. ------------------------------------------------------------------ Andrew Wallace, Ph.D, Queens University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://web.qub.ac.uk/bb/awpage/wallace.html From owner-repertoires@net.bio.net Wed Apr 30 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Revised list of meetings and courses Date: 1 May 1997 01:20:06 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 118 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5k9jjo$m7r@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net Meetings and courses on combinatorial libraries and phage display. ***************************************************************** _________________________________________________________________ *** MEETINGS *** _________________________________________________________________ Cambridge Healthtech Institute Fourth Annual HIGH-THROUGHPUT SCREENING June 25-27 Arlington, Virginia Cambridge Healthtech Institute 1037 Chestnut Street Newton Upper Falls, MA 02164 USA Phone: 617-630-1300 Fax: 617-630-1325 e-mail: chi@healthtech.com http://www.healthtech.com/conferences/ _________________________________________________________________ Cambridge Healthtech Institutes First European Conference on Combinatorial Chemistry and High Throughput Screening December 4-5, 1997 Hotel Arts Barcelona Barcelona, Spain Contact: Irene Phelan, Conference Director Phone: 617-630-1362 Fax: 617-630-1325 e-mail: iphelan@healthtech.com Organized by Cambridge Healthtech Institute 1037 Chestnut Steet, Newton Upper Falls, MA 02164 USA http://www/healthtech.com/conferences/ _________________________________________________________________ IBC's 2nd Annual Conference on Display Technologies: Advances in Protein Engineering, Drug/Vaccine Development and Molecular Evolution; August 4-5, 1997-Boston, MA For More Information: Call IBC USA Conferences at 508-481-6400, Fax: 508-481-4473 or email aheghinian@ibcusa.com for complete conference brochure or Register Online at http://www.ibcusa.com/conf/display _________________________________________________________________ Phage Display of Proteins and Peptides Biochemical Society Meeting University College Galway 3-5 September 1997 Deadline for poster registration 6 June 1997 Further information from: Michelle Mandale Meetings Office The Biochemical Society 59 Portland Place London W1N 3AJ Fax: (+44)171-637-7626 email: meetings@biochemsoc.org.uk _________________________________________________________________ *** COURSES *** _________________________________________________________________ Carolina Workshop on Combinatorial Molecules June 22 - 30, 1997 Theory and Applications of Combinatorial Libraries for Molecular Recognition Studies and Identification of Antagonists Tuition: $1200. Full consideration will be given to applications received by May 9, 1997. To Apply: Send a brief letter describing how this workshop will further your research project(s), along with your CV to the workshop coordinator: Wayne Litaker Program in Molecular Biology and Biotechnology University of North Carolina at Chapel Hill 442 Taylor Hall, CB # 7100 Chapel Hill, NC 27599-7100 office: (+1)919-929-0067 Fax: (+1)919-966-6821 email: litaker@med.unc.edu For additional information contact: briankay@email.unc.edu OR http://fordham25.bio.unc.edu/workshop.html __________________________________________________________________ PHAGE DISPLAY OF COMBINATORIAL ANTIBODY LIBRARIES Cold Spring Harbor Laboratory November 4 -17 , 1997 APPLICATION DEADLINE: July 15, 1997 Organisers: Carlos Barbas, Scripps Research Institute Dennis Burton, Scripps Research Institute Gregg Silverman, University of California, San Diego For Additional Information, please contact: Cold Spring Harbor Laboratory The Meetings & Courses Office 1 Bungtown Road, PO Box 100 Cold Spring Harbor, NY 11724-2213 USA Phone: (+1)516-367-8346 Fax: (+1)516-367-8845 email: meetings@cshl.org __________________________________________________________________ ------------------------------------------------------------------ Andrew Wallace, Ph.D, Queens University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://web.qub.ac.uk/bb/awpage/wallace.html From owner-repertoires@net.bio.net Wed Apr 30 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: M13K07 Date: 1 May 1997 10:01:36 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5kai36$21e@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net Since Peter Wang asked about VCSM13 helper phage, I was thinking about M13K07 itself. Is what I heard true that the sequence of the commercial material is different from the published sequence? What is the real sequence? Andrew -- ================================================================== Andrew Wallace,Ph.D., Queens University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://web.qub.ac.uk/bb/awpage/wallace.html ==================================================================