From owner-repertoires@net.bio.net Thu May 01 23:00:00 1997 Path: biosci!biosci!not-for-mail From: bradbury@icgeb.trieste.it (Andrew Bradbury) Newsgroups: bionet.molecules.repertoires Subject: Re: M13K07 Date: 2 May 1997 01:47:33 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 38 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5kc9db$c9@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net Dear Andrew, there is no published sequence. You can try and assemble it from the information given (I think it was published in a methods in enzymology), but the sequence you get out bears no resemblance whatsoever to the real thing in terms of restriction mapping. Basically, we don't know what M13K07 is. Even if someone did sequence it, I think that most people would be using M13K07 which would be different. best wishes andrew >Since Peter Wang asked about VCSM13 helper phage, I was thinking >about M13K07 itself. Is what I heard true that the sequence of the >commercial material is different from the published sequence? What is >the real sequence? > >Andrew >-- >================================================================== >Andrew Wallace,Ph.D., Queens University Belfast, N. Ireland (UK) >a.wallace@qub.ac.uk http://web.qub.ac.uk/bb/awpage/wallace.html >================================================================== Andrew Bradbury SISSA c/o ICGEB Area di Ricerca Padriciano 99 Trieste 34012 Italy Tel +39 40 398995 Fax +39 40 398991 From owner-repertoires@net.bio.net Wed May 07 23:00:00 1997 Path: biosci!biosci!not-for-mail From: M.Foley@latrobe.edu.au (Dr Michael Foley) Newsgroups: bionet.molecules.repertoires Subject: GFP in bacterial periplasms Date: 8 May 1997 01:44:58 -0700 Organization: La Trobe University Lines: 11 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5ks3qa$sdk@net.bio.net> NNTP-Posting-Host: net.bio.net Hi all, I was wondering if anyone has made GFP fusions with bacterial proteins located in the periplasm. Does it work? are they fluorescent? Cheers, Mick From owner-repertoires@net.bio.net Wed May 07 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: CLONTECH sponsors molreps! Date: 8 May 1997 06:30:30 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5ksgar$r23@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net I wanted to draw to everyone's attention that CLONTECH are now sponsoring the molreps message archive on the BioSci web server. See http://www.bio.net/hypermail/MOLECULAR-REPERTOIRES It gives me great pleasure to welcome CLONTECH in this venture and I thank them for their support. CLONTECH's web pages are at http://www.clontech.com/ Andrew ------------------------------------------------------------------ Andrew Wallace, Ph.D, Queens University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://web.qub.ac.uk/bb/awpage/wallace.html From owner-repertoires@net.bio.net Thu May 08 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "Dimitris K. Agrafiotis" Newsgroups: bionet.molecules.repertoires Subject: Job Openings at 3DP Date: 9 May 1997 02:27:13 -0700 Organization: 3-Dimensional Pharmaceuticals, Inc. Lines: 26 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5kuq6t$m6h@mserv1.dl.ac.uk> Reply-To: "Dimitris K. Agrafiotis" NNTP-Posting-Host: net.bio.net 3-Dimensional Pharmaceuticals, Inc. has several immediate openings for computational chemists and/or computer scientists to join the DirectedDiversity group. The successful candidates will participate in the development of state-of-the-art computational tools in the areas of molecular modeling, informatics and computational intelligence. Proficiency in C++ under UNIX and extensive experience in large scale software engineering, algorithm design, GUI development and design, and chemical computation is essential, particularly in the areas of protein-ligand interactions, molecular similarity, chemical diversity and QSAR. Experience with Java and Web programming is a plus. Positions are available at all levels (BS, MS, PhD). If you are interested, please send your resume by snail- or e-mail to the address indicated below. Thanks. -- Dimitris K. Agrafiotis, PhD | E-mail: dimitris@3dp.com 3-Dimensional Pharmaceuticals, Inc. | Tel: (610) 458-6045 665 Stockton Drive, Suite 104 | Fax: (610) 458-8249 Exton, PA 19341 From owner-repertoires@net.bio.net Thu May 08 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Stefan Unger Newsgroups: bionet.molecules.repertoires Subject: CONF FINAL PGM: MipTec '97 and Award Winners Announced Date: 9 May 1997 02:24:55 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 131 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5kuq6r$m6b@mserv1.dl.ac.uk> Reply-To: Stefan Unger NNTP-Posting-Host: net.bio.net CONFERENCE ANNOUNCEMENT FINAL PROGRAM AND ANNOUNCEMENT OF AWARD WINNERS MipTec '97: MicroPlate Technologies Hyatt Regency Crystal City Arlington, VA (Washington, DC) June 23-25, 1997 Recent progress in the use of 96-well, and the shift to 384-well, microplates in biomolecular discovery, genome research, chemical synthesis and biochemical analysis will be the focus of this important meeting. The conference will present frontline reports on recent data and results, with special emphasis on the practical aspects of new, high density screening methods: challenges met, solutions achieved, and new applications uncovered. There will be lectures, luncheon seminars and a technical exhibition (with about 50 exhibitors). Technical Program: * Microplate Designs and Applications (chairs: Keld Sorensen/Pierce and Roy Manns/Polyfiltronics) A. Vaheri/U. Helsinki, T. Beugelsdijk/Los Alamos, J. Seed/Advanced Genetic Technologies, M. Havsteen-Jakobsen/Exiqon, R. Buckheit Jr/So. Res. Inst., D. Sponholtz/Dynex, J. Robbins/Whatman, J. Jolly et al/Molecular Biology Resources, W. Lipton et al/Pierce, J. Lampinen/Bio-Orbit * Microplate Design and Handling characteristics (chairs: Rob Zambias/TexPerts) D. Root/Corning Costar, J. Latham/Darwin, B. Young/Dynex, T. Astle/TomTec, K. Gregorius et al/M&E A/S, G. Knebel/Greiner Labortechnik * The 1997 PolyWhat Award and Lecture: presented to Dr. Greg Lennon, Lawrence Livermore Laboratory The PolyWhat Award recognizes academic innovation in the design or use of microplates. Dr. Lennon is the originator of the 384 square well plate. He is also noted for its application in the storage of cDNA libraries within the I.M.A.G.E. Consortium, of which he is a founder. The Award will be presented by Dr. Roy Manns (President of Polyfiltronics). * Applications in Genome Projects (chair: Jim Stanchfield/Robbins Scientific) R. McIndoe/U. Wash., E. Stewart/Stanford HGC, K. Konrad/Incyte, H. Garner/U. Texas, J. Stanchfield/Robbins Scientific, A. Copeland et al/Lawrence Livermore, G. Walter et al/Max Planck Inst. Mol. Genetics * Luncheon Seminars by Molecular Devices Corporation and Spectronic Instruments * Robotics and Laboratory Automation (chairs: Al Kolb/Packard and Robin Felder/U. Virginia) J. Major/Zeneca, J. Houston/Glaxo, M-J. Widey/RWJ PRI, T. Garyantes/Merck, T. Jean/Cerap, R. Papen et al/Packard, S. Fogarty et al/GlaxoWellcome, B. Brown et al/NEN Life Sciences, R. Felder/U. Virginia * Informatics and Database Integration (chair: Michael Liebman/Vysis) L. Kauvar/Terrapin, B. Cocks/INcyte, U Muller et al/Vysis * Biomolecular Screening and Library Generation I (chair: John Devlin) J. Devlin/ARRT Intl., C. Goddard/Oncogene, M. Conrad/Chromogen * Luncheon Seminars by Packard Instrument Company and ID Business Solutions * Biomolecular Screening and Library Generation II (chair: J. Cook/Procter & Gamble) A. Pakula/Scriptgen, B. Hynd/Procter & Gamble, M. Crawford/Lasure & Crawford, J. Comley/GlaxoWellcome, M. Mitsuhashi et al/U. Calif. Irvine and Hitachi Chemical * The CHARB Award and Lecture: presented to Dr. Chris Roelant, U Ziekenhuizen Leuven The CHARB Award recognizes academic innovation in the development of HTS technologies and/or their implementation into the Academic curriculum. Dr. Roelant has made important contributions in assay miniaturization, cell-based assays in 384 well microplates and the development of unique glow luminescence reagents for HTS. The Award will be presented on Wednesday afternoon by Dr. John Devlin (on behalf of Cambridge Healthtech, ARRT International and Bureco). MipTec '97 immediately preceeds The Fourth Annual Conference on High Throughput Screening for Drug Discovery by Cambridge Healthtech Institute, June 25-27, 1997 with sessions on Natural Products, Rapid Compound Characterization, Combinatorial Libraries, Laboratory Automation, Miniaturization and Screening Programs. Registration for MipTec '97 includes attendance at all conference sessions, receptions, breaks, snack lunches and access to the Tradeshow and Luncheon Seminars. Hotel accomodations are the responsibility of the participant. For fees and further information, please contact: John Devlin, PhD, ARRT International, Inc. PO Box 1838 11 Main St New Milford, CT 06776 USA Phone: 860-355-5195 Fax: 860-355-5975 E-mail: arrtintl@prodigy.net @@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@@#@@#@@#@@#@@#@@#@@#@@# Stefan Unger, Ph.D. President BioSoftware Marketing 4151 Middlefield Rd., Ste 109 Palo Alto, CA 94303-4743 ph: 415-858-0522 fx: 415-858-0521 email: sunger@a.crl.com http://www.bio.com/home/bsm/bsm.html BioSoftware Marketing specializes in a full range of marketing services (market studies to collateral design) for technical software and hardware companies. E-mail your full postal mailing address to receive a full-color brochure. Product Manager, Scientific Software, Bio Online Store (http://www.bio.com/) @@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@@#@@#@@#@@#@@#@@#@@# From owner-repertoires@net.bio.net Thu May 08 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Athel Cornish-Bowden Newsgroups: bionet.molecules.repertoires Subject: IUBMB is trying to end enzyme classification Date: 9 May 1997 07:28:48 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 33 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5kvbur$eln@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net Do you use any of these databases (or other similar ones) to find information about enzymes or metabolic pathways? Biocatalysis/biodegradation database (http://dragon.labmed.umn.edu/~lynda/index.html)? EcoCyc (http://www.ai.sri.com/ecocyc/ecocyc.html)? GenoBase (http://specter.dcrt.nih.gov:8004/Pathway/pathway_toc_by_name.html)? KEGG (http://www.genome.ad.jp/kegg/kegg1.html)? SoyBase (http://probe.nal.usda.gov:8000/plant/aboutsoybase.html)? SwissProt (http://www.expasy.ch/sprot/sprot-top.html)? WIT (What is there?) (http://www.cme.msu.edu/WIT/)? If so you will know how much they depend on the EC classification system for enzymes sponsored (until now) by IUBMB. You will also be concerned by the fact that the IUBMB Executive Committee has taken a decision that if not reversed will make it impossible for this work to continue. Full information, including suggestions of what you can do about it, is given on a web page at http://www.chem.qmw.ac.uk/iupac/jcbn/help.html. Athel Cornish-Bowden From owner-repertoires@net.bio.net Mon May 12 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Update on meetings and courses 1997-1998 Date: 13 May 1997 01:57:16 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 155 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5l99oq$lo7@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net Meetings and courses on combinatorial libraries and phage display. ***************************************************************** _________________________________________________________________ *** MEETINGS *** _________________________________________________________________ MipTec '97: MicroPlate Technologies Hyatt Regency Crystal City Arlington, VA (Washington, DC) June 23-25, 1997 MipTec '97 immediately preceeds The Fourth Annual Conference on High Throughput Screening for Drug Discovery by Cambridge Healthtech Institute, June 25-27, 1997. Registration for MipTec '97 includes attendance at all conference sessions, receptions, breaks, snack lunches and access to the Tradeshow and Luncheon Seminars. Hotel accomodations are the responsibility of the participant. For fees and further information, please contact: John Devlin, PhD, ARRT International, Inc. PO Box 1838 11 Main St New Milford, CT 06776 USA. e-Mail: arrtintl@prodigy.net _________________________________________________________________ Cambridge Healthtech Institute Fourth Annual HIGH-THROUGHPUT SCREENING June 25-27 Arlington, Virginia Cambridge Healthtech Institute 1037 Chestnut Street Newton Upper Falls, MA 02164 USA Phone: 617-630-1300 Fax: 617-630-1325 e-mail: chi@healthtech.com http://www.healthtech.com/conferences/ _________________________________________________________________ Cambridge Healthtech Institutes First European Conference on Combinatorial Chemistry and High Throughput Screening December 4-5, 1997 Hotel Arts Barcelona Barcelona, Spain Contact: Irene Phelan, Conference Director Phone: 617-630-1362 Fax: 617-630-1325 e-mail: iphelan@healthtech.com Organized by Cambridge Healthtech Institute 1037 Chestnut Steet, Newton Upper Falls, MA 02164 USA http://www/healthtech.com/conferences/ _________________________________________________________________ IBC's 2nd Annual Conference on Display Technologies: Advances in Protein Engineering, Drug/Vaccine Development and Molecular Evolution; August 4-5, 1997-Boston, MA For More Information: Call IBC USA Conferences at 508-481-6400, Fax: 508-481-4473 or email aheghinian@ibcusa.com for complete conference brochure or Register Online at http://www.ibcusa.com/conf/display _________________________________________________________________ Phage Display of Proteins and Peptides Biochemical Society Meeting University College Galway 3-5 September 1997 Deadline for poster registration 6 June 1997 Further information from: Michelle Mandale Meetings Office The Biochemical Society 59 Portland Place London W1N 3AJ Fax: (+44)171-637-7626 email: meetings@biochemsoc.org.uk __________________________________________________________________ The 8th Cyprus Conference on New Methods in Drug Research Limassol, Cyprus May 4-9, 1998 Conference Executive: Chair: Alex Makriyannis (U. Conn. Storrs, USA) Committee: N. Castagnoli (USA), J.P. Devlin (USA), U. Hacksell (Sweden), L.H. Hurley (USA) and M. Abou-Gharbia (USA) Conference Location Time and Registration: The Conference will take place from Monday, May 4th through Friday, May 9th 1998 at the five-star Hawaii Beach Hotel, Limassol, Cyprus. Attendance is restricted to 150 participants to encourage interaction. Registration fees ($595 per person; $395 for academics) includes attendance at all sessions and morning and afternoon breaks, but not meals. Sponsor: The European Federation of Medicinal Chemistry Contact: ARRT International, Inc., P.O. Box 1838, New Milford, CT 06776, USA Telephone: 860-355-5195; Facsimile: 860-355-5975; e-Mail: arrtintl@prodigy.net _________________________________________________________________ *** COURSES *** _________________________________________________________________ Carolina Workshop on Combinatorial Molecules June 22 - 30, 1997 Theory and Applications of Combinatorial Libraries for Molecular Recognition Studies and Identification of Antagonists Tuition: $1200. Full consideration will be given to applications received by May 9, 1997. To Apply: Send a brief letter describing how this workshop will further your research project(s), along with your CV to the workshop coordinator: Wayne Litaker Program in Molecular Biology and Biotechnology University of North Carolina at Chapel Hill 442 Taylor Hall, CB # 7100 Chapel Hill, NC 27599-7100 office: (+1)919-929-0067 Fax: (+1)919-966-6821 email: litaker@med.unc.edu For additional information contact: briankay@email.unc.edu OR http://fordham25.bio.unc.edu/workshop.html __________________________________________________________________ PHAGE DISPLAY OF COMBINATORIAL ANTIBODY LIBRARIES Cold Spring Harbor Laboratory November 4 -17 , 1997 APPLICATION DEADLINE: July 15, 1997 Organisers: Carlos Barbas, Scripps Research Institute Dennis Burton, Scripps Research Institute Gregg Silverman, University of California, San Diego For Additional Information, please contact: Cold Spring Harbor Laboratory The Meetings & Courses Office 1 Bungtown Road, PO Box 100 Cold Spring Harbor, NY 11724-2213 USA Phone: (+1)516-367-8346 Fax: (+1)516-367-8845 email: meetings@cshl.org __________________________________________________________________ ------------------------------------------------------------------ Andrew Wallace, Ph.D, Queens University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://web.qub.ac.uk/bb/awpage/wallace.html From owner-repertoires@net.bio.net Mon May 12 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Stefan Unger Newsgroups: bionet.molecules.repertoires Subject: CONF: 8th Cyprus Conference on New Methods in Drug Research Date: 13 May 1997 01:55:33 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 92 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5l99p6$lo2@mserv1.dl.ac.uk> Reply-To: Stefan Unger NNTP-Posting-Host: net.bio.net The 8th Cyprus Conference on New Methods in Drug Research Limassol, Cyprus May 4-9, 1998 Conference Executive: Chair: Alex Makriyannis (U. Conn. Storrs, USA) Committee: N. Castagnoli (USA), J.P. Devlin (USA), U. Hacksell (Sweden), L.H. Hurley (USA) and M. Abou-Gharbia (USA) For the past seventeen years, this classic event has served as the biennial opportunity for researchers and management executives to interact, exchange views and philosophies, preview cutting edge technologies in Drug Discovery and get a glimpse of things to come. The format is the traditional Gordon-style enhanced by the historical ambiance of Cyprus! Both the scientific papers and the interests of the participants are multidisciplinary and this mix, inevitably, results in innovative new approaches and collaborations. Half Day Technical Sessions: * New Roles for Synthetic Chemistry in Drug Design (Chair: Steve Hanessian (U. Montreal)) * Advances in Drug Receptor Research (Chair: Hendrik Timmerman (Amsterdam Center for Drug Research)) * The Use of Computational, Biophysical and Biochemical Methods (Chairs: Stephen Fesik (Abbott), Alex Makriyannis (U. Conn. Storrs)) * Ultra-High Throughput Technologies in Discovery and Development (Chair: Al Kolb (Packard Instrument)) * Micro- and Nanotechnologies in Measuring Biomolecular Interactions (Chair: Herman Gaub (Ludwig Maximilians Univ) * Computational-HTS Integration in Drug Discovery (Round Table Discussion) * Genomics-based Approaches in Discovery and Structure-Function Correlations (Chair: Casey Case (Tularik)) * Novel Design Approaches in Combating Drug Resistance (Chair: Lawrence Melvin (Amgen)) Conference Location Time and Registration: The Conference will take place from Monday, May 4th through Friday, May 9th 1998 at the five-star Hawaii Beach Hotel, Limassol, Cyprus. Attendance is restricted to 150 participants to encourage interaction. Registration fees ($595 per person; $395 for academics) includes attendance at all sessions and morning and afternoon breaks, but not meals. Local accommodations and air/ground travel expenses are the responsibility of the participant and can be arranged at the time of registration. Post conference tours are also available; please inquire. An important event, both as a refreshing midweek break and as an opportunity for casual and productive collegial interactions is the traditional Wednesday excursion to historical and archeological sites of interest followed by a memorable evening of Cypriot fare and entertainment. (Optional with separage charge). Sponsor: The European Federation of Medicinal Chemistry Contact: ARRT International, Inc., P.O. Box 1838, New Milford, CT 06776, USA Telephone: 860-355-5195; Facsimile: 860-355-5975; e-Mail: arrtintl@prodigy.net @@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@@#@@#@@#@@#@@#@@#@@#@@# Stefan Unger, Ph.D. President BioSoftware Marketing 4151 Middlefield Rd., Ste 109 Palo Alto, CA 94303-4743 ph: 415-858-0522 fx: 415-858-0521 email: sunger@a.crl.com http://www.bio.com/home/bsm/bsm.html BioSoftware Marketing specializes in a full range of marketing services (market studies to collateral design) for technical software and hardware companies. E-mail your full postal mailing address to receive a full-color brochure. Product Manager, Scientific Software, Bio Online Store (http://www.bio.com/) @@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@#@@@#@@#@@#@@#@@#@@#@@# From owner-repertoires@net.bio.net Thu May 15 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "Janet Clench, Library, Tel:(39 6)91093220" Newsgroups: bionet.molecules.repertoires Subject: and at long last ... Date: 16 May 1997 05:00:14 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 234 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5lhi8e$scr@net.bio.net> NNTP-Posting-Host: net.bio.net ******************************************************** ****** SUBJECT: Combinatorial & Phage Libraries DATE: April 14, 21, 28 1997 ******************************************************** ****** Papavoine, C.H.M.; Remerowski, M.L.; Horstink, L.M.; Konings, R.N.H.; Hilbers, C.W.; vandeVen, F.J.M. Backbone dynamics of the major coat protein of bacteriophage M13 in detergent micelles by N-15 nuclear magnetic resonance relaxation measurements using the model-free approach and reduced spectral density mapping Biochemistry 36: 13 (APR 1 1997) 4015-4026 Adamczyk, M.; Gebler, J.C.; Gunasekera, A.H.; Mattingly, P.G.; Pan, Y. Immunoassay reagents for thyroid testing .2. Binding properties and energetic parameters of a T-4 monoclonal antibody and its fab fragment with a library of thyroxine analog biosensors using surface plasmon resonance Bioconjugate Chemistry 8: 2 (MAR-APR 1997) 133-145 Zhang, H.L.; Zhong, Z.J.; Pirofski, L.A. Peptide epitopes recognized by a human anti-cryptococcal glucuronoxylomannan antibody Infection and Immunity 65: 4 (APR 1997) 1158-1164 Murray, C.W.; Clark, D.E.; Auton, T.R.; Firth, M.A.; Li, J.; Sykes, R.A.; Waszkowycz, B.; Westhead, D.R.; Young, S.C. PRO_SELECT: Combining structure-based drug design and combinatorial chemistry for rapid lead discovery .1. Technology Journal of Computer - Aided Molecular Design 11: 2 (MAR 1997) 193-207 Tomandl, D.; Schober, A.; Schwienhorst, A. Optimizing doped libraries by using genetic algorithms Journal of Computer - Aided Molecular Design 11: 1 (JAN 1997) 29-38 Gold, L.; Alper, J. Drug discovery - Keeping pace with genomics through combinatorial chemistry Nature Biotechnology 15: 4 (APR 1997) 297-297 Hogan, J.C. Combinatorial chemistry in drug discovery Nature Biotechnology 15: 4 (APR 1997) 328-330 Stricker, N.L.; Christopherson, K.S.; Yi, B.A.; Schatz, P.J.; Raab, R.W.; Dawes, G.; Bassett, D.E.; Bredt, D.S.; Li, M. PDZ domain of neuronal nitric oxide synthase recognizes novel C- terminal peptide sequences Nature Biotechnology 15: 4 (APR 1997) 336-342 Persidis, A. Combinatorial chemistry Nature Biotechnology 15: 4 (APR 1997) 391-392 Salemme, F.R.; Spurlino, J.; Bone, R. Serendipity meets precision: The integration of structure-based drug design and combinatorial chemistry for efficient drug discovery Structure 5: 3 (MAR 15 1997) 319-324 Esposito, G.; Morea, V.; Scarselli, E.; Cerino, A.; Mondelli, M.U.; LaMonica, N.; Traboni, C. Recombinant human antibodies specific for hepatitis C virus proteins Archives of Virology 142: 3 (1997) 601-610 Gersuk, G.M.; Corey, M.J.; Corey, E.; Stray, J.E.; Kawasaki, G.H.; Vessella, R.L. High-affinity peptide ligands to prostate-specific antigen identified by polysome selection Biochemical and Biophysical Research Communications 232: 2 (MAR 17 1997) 578-582 Pajunen, M.; Saviranta, P.; Jauria, P.; Karp, M.; Pettersson, K.; Mantsala, P.; Lovgren, T. Cloning, sequencing, expression and characterization of three anti- estradiol-17 beta Fab fragments Biochimica et Biophysica Acta - Gene Structure and Expression 1351: 1-2 (MAR 20 1997) 192-202 Rano, T.A.; Timkey, T.; Peterson, E.P.; Rotonda, J.; Nicholson, D.W.; Becker, J.W.; Chapman, K.T.; Thornberry, N.A. A combinatorial approach for determining protease specificities: Application to interleukin-1 beta converting enzyme (ICE) Chemistry & Biology 4: 2 (FEB 1997) 149-155 Lasters, I.; VanHerzeele, N.; Lijnen, H.R.; Collen, D.; Jespers, L. Enzymatic properties of phage-displayed fragments of human plasminogen European Journal of Biochemistry 244: 3 (MAR 15 1997) 946-952 Pereira, S.; VanBelle, P.; Elder, D.; Maruyama, H.; Jacob, L.; Sivanandham, M.; Wallack, M.; Siegel, D.; Herlyn, D. Combinatorial antibodies against human malignant melanoma Hybridoma 16: 1 (FEB 1997) 11-16 Schlebusch, H.; Reinartz, S.; Kaiser, R.; Grunn, U.; Wagner, U. Production of a single-chain fragment of the murine anti-idiotypic antibody ACA125 as phage-displayed and soluble antibody by recombinant phage antibody technique Hybridoma 16: 1 (FEB 1997) 47-52 Zhang, H.C.; Maryanoff, B.E. Construction of indole and benzofuran systems on the solid phase via palladium-mediated cyclizations Journal of Organic Chemistry 62: 6 (MAR 21 1997) 1804-1809 Huc, I.; Lehn, J.M. Virtual combinatorial libraries: Dynamic generation of molecular and supramolecular diversity by self-assembly Proceedings of the National Academy of Sciences of the United States of America 94: 6 (MAR 18 1997) 2106-2110 Harris, S.L.; Craig, L.; Mehroke, J.S.; Rashed, M.; Zwick, M.B.; Kenar, K.; Toone, E.J.; Greenspan, N.; Auzanneau, F.I.; MarinoAlbernas, J.R.; Pinto, B.M.; Scott, J.K. Exploring the basis of peptide-carbohydrate crossreactivity: Evidence for discrimination by peptides between closely related anti-carbohydrate antibodies Proceedings of the National Academy of Sciences of the United States of America 94: 6 (MAR 18 1997) 2454-2459 Burioni, R.; Plaisant, P.; DelliCarri, V.; Vannini, A.; Spanu, T.; Clementi, M.; Fadda, G.; Varaldo, P.E. An improved phage display vector for antibody repertoire cloning by construction of combinatorial libraries Research in Virology 148: 2 (MAR-APR 1997) 161-164 Plaisant, P.; Burioni, R.; Manzin, A.; Solforosi, L.; Candela, M.; Gabrielli, A.; Fadda, G.; Clementi, M. Human monoclonal recombinant Fabs specific for HCV antigens obtained by repertoire cloning in phage display combinatorial vectors Research in Virology 148: 2 (MAR-APR 1997) 165-169 Plunkett, M.J.; Ellman, J.A. Combinatorial chemistry and new drugs Scientific American 276: 4 (APR 1997) 68-73 Rich, D.H.; Bohacek, R.S.; Dales, N.A.; Glunz, P.; Ripka, A.S. Transformation of peptides into non-peptides. Synthesis of computer- generated enzyme inhibitors Chimia 51: 1-2 (JAN-FEB 1997) 45-47 Nuss, J.M.; Desai, M.C.; Zuckermann, R.N.; Singh, R.; Renhowe, P.A.; Goff, D.A.; Chinn, J.P.; Wang, L.; Dorr, H.; Brown, E.G.; Subramanian, S. Developing a general strategy for the solid supported synthesis of heterocycles: Applications to the generation of molecular diversity and drug discovery Pure and Applied Chemistry 69: 3 (MAR 1997) 447-452 Zaccolo, M.; Griffiths, A.P.; Prospero, T.D.; Winter, G.; Gherardi, E. Dimerization of fab fragments enables ready screening of phage antibodies that affect hepatocyte growth factor scatter factor activity on target cells European Journal of Immunology 27: 3 (MAR 1997) 618-623 Persic, L.; Righi, M.; Roberts, A.; Hoogenboom, H.R.; Cattaneo, A.; Bradbury, A. Targeting vectors for intracellular immunisation Gene 187: 1 (MAR 10 1997) 1-8 Persic, L.; Roberts, A.; Wilton, J.; Cattaneo, A.; Bradbury, A.; Hoogenboom, H.R. An integrated vector system for the eukaryotic expression of antibodies or their fragments after selection from phage display libraries Gene 187: 1 (MAR 10 1997) 9-18 Sawyer, J.R. Antibody fragments generated by phage display for use in diagnostic immunoassay Journal of Clinical Ligand Assay 20: 1 (SPR 1997) 150-151 Daefler, S.; Russel, M.; Model, P. Module swaps between related translocator proteins pIV(f1), pIV(IKe) and PulD: Identification of a specificity domain Journal of Molecular Biology 266: 5 (MAR 14 1997) 978-992 Boojamra, C.G.; Burow, K.M.; Thompson, L.A.; Ellman, J.A. Solid-phase synthesis of 1,4-benzodiazepine-2,5-diones. Library preparation and demonstration of synthesis generality Journal of Organic Chemistry 62: 5 (MAR 7 1997) 1240-1256 Chen, C.Z.; Xia, Q.C.; Li, B.L.; Wang, Y.L. Selection of substrate recognition sequence of protein kinase with ferric chelation affinity chromatography Science in China Series C - Life Sciences 40: 2 (APR 1997) 184-193 Wang, G.T.; Chen, Y.W.; Wang, S.D.; Sciotti, R.; Sowin, T. Solid phase synthesis of ureas of secondary amines via carbamoyl chloride Tetrahedron Letters 38: 11 (MAR 17 1997) 1895-1898 Nielsen, J.; Jensen, F.R. Solid-phase synthesis of small molecule libraries using double combinatorial chemistry Tetrahedron Letters 38: 11 (MAR 17 1997) 2011-2014 From owner-repertoires@net.bio.net Mon May 19 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Phage display cDNA system Date: 20 May 1997 10:04:24 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5lsj3j$m0b@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net Does anyone have experience with the phage display cDNA library system from Clontech? As far as I can tell, their phagemid vector displays the cDNA product at the C-terminus of pIII. How effective is this compared to other display systems such as pVI display or the pJuFo method? Andrew -- ================================================================== Andrew Wallace,Ph.D., Queens University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://web.qub.ac.uk/bb/awpage/wallace.html ================================================================== From owner-repertoires@net.bio.net Tue May 20 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "Janet Clench, Library, Tel:(39 6)91093220" Newsgroups: bionet.molecules.repertoires Subject: and at long last ... Date: 21 May 1997 02:04:14 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5lhfn9$88@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net [Apologies for any duplication, this message had an error when it was first sent - Andrew] ******************************************************** ****** SUBJECT: Combinatorial & Phage Libraries DATE: April 14, 21, 28 1997 ******************************************************** ****** Papavoine, C.H.M.; Remerowski, M.L.; Horstink, L.M.; Konings, R.N.H.; Hilbers, C.W.; vandeVen, F.J.M. Backbone dynamics of the major coat protein of bacteriophage M13 in detergent micelles by N-15 nuclear magnetic resonance relaxation measurements using the model-free approach and reduced spectral density mapping Biochemistry 36: 13 (APR 1 1997) 4015-4026 Adamczyk, M.; Gebler, J.C.; Gunasekera, A.H.; Mattingly, P.G.; Pan, Y. Immunoassay reagents for thyroid testing .2. Binding properties and energetic parameters of a T-4 monoclonal antibody and its fab fragment with a library of thyroxine analog biosensors using surface plasmon resonance Bioconjugate Chemistry 8: 2 (MAR-APR 1997) 133-145 Zhang, H.L.; Zhong, Z.J.; Pirofski, L.A. Peptide epitopes recognized by a human anti-cryptococcal glucuronoxylomannan antibody Infection and Immunity 65: 4 (APR 1997) 1158-1164 Murray, C.W.; Clark, D.E.; Auton, T.R.; Firth, M.A.; Li, J.; Sykes, R.A.; Waszkowycz, B.; Westhead, D.R.; Young, S.C. PRO_SELECT: Combining structure-based drug design and combinatorial chemistry for rapid lead discovery .1. Technology Journal of Computer - Aided Molecular Design 11: 2 (MAR 1997) 193-207 Tomandl, D.; Schober, A.; Schwienhorst, A. Optimizing doped libraries by using genetic algorithms Journal of Computer - Aided Molecular Design 11: 1 (JAN 1997) 29-38 Gold, L.; Alper, J. Drug discovery - Keeping pace with genomics through combinatorial chemistry Nature Biotechnology 15: 4 (APR 1997) 297-297 Hogan, J.C. Combinatorial chemistry in drug discovery Nature Biotechnology 15: 4 (APR 1997) 328-330 Stricker, N.L.; Christopherson, K.S.; Yi, B.A.; Schatz, P.J.; Raab, R.W.; Dawes, G.; Bassett, D.E.; Bredt, D.S.; Li, M. PDZ domain of neuronal nitric oxide synthase recognizes novel C- terminal peptide sequences Nature Biotechnology 15: 4 (APR 1997) 336-342 Persidis, A. Combinatorial chemistry Nature Biotechnology 15: 4 (APR 1997) 391-392 Salemme, F.R.; Spurlino, J.; Bone, R. Serendipity meets precision: The integration of structure-based drug design and combinatorial chemistry for efficient drug discovery Structure 5: 3 (MAR 15 1997) 319-324 Esposito, G.; Morea, V.; Scarselli, E.; Cerino, A.; Mondelli, M.U.; LaMonica, N.; Traboni, C. Recombinant human antibodies specific for hepatitis C virus proteins Archives of Virology 142: 3 (1997) 601-610 Gersuk, G.M.; Corey, M.J.; Corey, E.; Stray, J.E.; Kawasaki, G.H.; Vessella, R.L. High-affinity peptide ligands to prostate-specific antigen identified by polysome selection Biochemical and Biophysical Research Communications 232: 2 (MAR 17 1997) 578-582 Pajunen, M.; Saviranta, P.; Jauria, P.; Karp, M.; Pettersson, K.; Mantsala, P.; Lovgren, T. Cloning, sequencing, expression and characterization of three anti- estradiol-17 beta Fab fragments Biochimica et Biophysica Acta - Gene Structure and Expression 1351: 1-2 (MAR 20 1997) 192-202 Rano, T.A.; Timkey, T.; Peterson, E.P.; Rotonda, J.; Nicholson, D.W.; Becker, J.W.; Chapman, K.T.; Thornberry, N.A. A combinatorial approach for determining protease specificities: Application to interleukin-1 beta converting enzyme (ICE) Chemistry & Biology 4: 2 (FEB 1997) 149-155 Lasters, I.; VanHerzeele, N.; Lijnen, H.R.; Collen, D.; Jespers, L. Enzymatic properties of phage-displayed fragments of human plasminogen European Journal of Biochemistry 244: 3 (MAR 15 1997) 946-952 Pereira, S.; VanBelle, P.; Elder, D.; Maruyama, H.; Jacob, L.; Sivanandham, M.; Wallack, M.; Siegel, D.; Herlyn, D. Combinatorial antibodies against human malignant melanoma Hybridoma 16: 1 (FEB 1997) 11-16 Schlebusch, H.; Reinartz, S.; Kaiser, R.; Grunn, U.; Wagner, U. Production of a single-chain fragment of the murine anti-idiotypic antibody ACA125 as phage-displayed and soluble antibody by recombinant phage antibody technique Hybridoma 16: 1 (FEB 1997) 47-52 Zhang, H.C.; Maryanoff, B.E. Construction of indole and benzofuran systems on the solid phase via palladium-mediated cyclizations Journal of Organic Chemistry 62: 6 (MAR 21 1997) 1804-1809 Huc, I.; Lehn, J.M. Virtual combinatorial libraries: Dynamic generation of molecular and supramolecular diversity by self-assembly Proceedings of the National Academy of Sciences of the United States of America 94: 6 (MAR 18 1997) 2106-2110 Harris, S.L.; Craig, L.; Mehroke, J.S.; Rashed, M.; Zwick, M.B.; Kenar, K.; Toone, E.J.; Greenspan, N.; Auzanneau, F.I.; MarinoAlbernas, J.R.; Pinto, B.M.; Scott, J.K. Exploring the basis of peptide-carbohydrate crossreactivity: Evidence for discrimination by peptides between closely related anti-carbohydrate antibodies Proceedings of the National Academy of Sciences of the United States of America 94: 6 (MAR 18 1997) 2454-2459 Burioni, R.; Plaisant, P.; DelliCarri, V.; Vannini, A.; Spanu, T.; Clementi, M.; Fadda, G.; Varaldo, P.E. An improved phage display vector for antibody repertoire cloning by construction of combinatorial libraries Research in Virology 148: 2 (MAR-APR 1997) 161-164 Plaisant, P.; Burioni, R.; Manzin, A.; Solforosi, L.; Candela, M.; Gabrielli, A.; Fadda, G.; Clementi, M. Human monoclonal recombinant Fabs specific for HCV antigens obtained by repertoire cloning in phage display combinatorial vectors Research in Virology 148: 2 (MAR-APR 1997) 165-169 Plunkett, M.J.; Ellman, J.A. Combinatorial chemistry and new drugs Scientific American 276: 4 (APR 1997) 68-73 Rich, D.H.; Bohacek, R.S.; Dales, N.A.; Glunz, P.; Ripka, A.S. Transformation of peptides into non-peptides. Synthesis of computer- generated enzyme inhibitors Chimia 51: 1-2 (JAN-FEB 1997) 45-47 Nuss, J.M.; Desai, M.C.; Zuckermann, R.N.; Singh, R.; Renhowe, P.A.; Goff, D.A.; Chinn, J.P.; Wang, L.; Dorr, H.; Brown, E.G.; Subramanian, S. Developing a general strategy for the solid supported synthesis of heterocycles: Applications to the generation of molecular diversity and drug discovery Pure and Applied Chemistry 69: 3 (MAR 1997) 447-452 Zaccolo, M.; Griffiths, A.P.; Prospero, T.D.; Winter, G.; Gherardi, E. Dimerization of fab fragments enables ready screening of phage antibodies that affect hepatocyte growth factor scatter factor activity on target cells European Journal of Immunology 27: 3 (MAR 1997) 618-623 Persic, L.; Righi, M.; Roberts, A.; Hoogenboom, H.R.; Cattaneo, A.; Bradbury, A. Targeting vectors for intracellular immunisation Gene 187: 1 (MAR 10 1997) 1-8 Persic, L.; Roberts, A.; Wilton, J.; Cattaneo, A.; Bradbury, A.; Hoogenboom, H.R. An integrated vector system for the eukaryotic expression of antibodies or their fragments after selection from phage display libraries Gene 187: 1 (MAR 10 1997) 9-18 Sawyer, J.R. Antibody fragments generated by phage display for use in diagnostic immunoassay Journal of Clinical Ligand Assay 20: 1 (SPR 1997) 150-151 Daefler, S.; Russel, M.; Model, P. Module swaps between related translocator proteins pIV(f1), pIV(IKe) and PulD: Identification of a specificity domain Journal of Molecular Biology 266: 5 (MAR 14 1997) 978-992 Boojamra, C.G.; Burow, K.M.; Thompson, L.A.; Ellman, J.A. Solid-phase synthesis of 1,4-benzodiazepine-2,5-diones. Library preparation and demonstration of synthesis generality Journal of Organic Chemistry 62: 5 (MAR 7 1997) 1240-1256 Chen, C.Z.; Xia, Q.C.; Li, B.L.; Wang, Y.L. Selection of substrate recognition sequence of protein kinase with ferric chelation affinity chromatography Science in China Series C - Life Sciences 40: 2 (APR 1997) 184-193 Wang, G.T.; Chen, Y.W.; Wang, S.D.; Sciotti, R.; Sowin, T. Solid phase synthesis of ureas of secondary amines via carbamoyl chloride Tetrahedron Letters 38: 11 (MAR 17 1997) 1895-1898 Nielsen, J.; Jensen, F.R. Solid-phase synthesis of small molecule libraries using double combinatorial chemistry Tetrahedron Letters 38: 11 (MAR 17 1997) 2011-2014 From owner-repertoires@net.bio.net Tue May 20 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Mark Suter Newsgroups: bionet.molecules.repertoires Subject: Re: Phage display cDNA system Date: 21 May 1997 06:36:42 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 46 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5lupgh$lec@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net >Andrew Wallace wrote: >> >> Does anyone have experience with the phage display cDNA library system >> from Clontech? As far as I can tell, their phagemid vector displays the >> cDNA product at the C-terminus of pIII. How effective is this compared >> to other display systems such as pVI display or the pJuFo method? >> >> Andrew > >It seems that I was wrong in my assumption about the construction of the >display vector. The cDNA library is cloned in at the N-terminus of pIII, >which makes sense in trying to achieve display, but then the actual >display of cDNA products will be reduced due to frameshifts and >missense mutations from introducing the cDNA affecting the expression >of pIII and therefore functional phage, right? Yes, the correction is right. Your concern was the reason in 1993 to develope pJuFo to be able to: - clone cDNA directional (still there are three reading frames) - most importanly poly A does not matter - several enzymes to cut (but still int 5'- 3'orientation) Mark > >Andrew >-- >------------------------------------------------------------------ >Andrew Wallace, Ph.D, Queens University Belfast, N. Ireland (UK) >a.wallace@qub.ac.uk http://web.qub.ac.uk/bb/awpage/wallace.html \|/ (o o) ________________________________oOo__(_)__oOo_________________________________ ___/\_ | Mark Suter mailto:msuter@vetvir.unizh.ch / o \/| | University Inst.for Virology http://www.unizh.ch/vetvir / _| | Winterthurerstr. 266a Telephone: (+41) 1 6358717 /_/\__/-\/ | 8057 Zurich SWITZERLAND Faximile : (+41) 1 6358911 ______________________________________________________________________________ If it wasn't for Windows, we wouldn't have anything to compare Mac's to ______________________________________________________________________________ From owner-repertoires@net.bio.net Tue May 20 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Re: Phage display cDNA system Date: 21 May 1997 02:33:50 -0700 Organization: Queens University Belfast Lines: 23 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5luf53$5la@mserv1.dl.ac.uk> References: <5lsj3j$m0b@mserv1.dl.ac.uk> Reply-To: a.wallace@Queens-Belfast.ac.uk NNTP-Posting-Host: net.bio.net Andrew Wallace wrote: > > Does anyone have experience with the phage display cDNA library system > from Clontech? As far as I can tell, their phagemid vector displays the > cDNA product at the C-terminus of pIII. How effective is this compared > to other display systems such as pVI display or the pJuFo method? > > Andrew It seems that I was wrong in my assumption about the construction of the display vector. The cDNA library is cloned in at the N-terminus of pIII, which makes sense in trying to achieve display, but then the actual display of cDNA products will be reduced due to frameshifts and missense mutations from introducing the cDNA affecting the expression of pIII and therefore functional phage, right? Andrew -- ------------------------------------------------------------------ Andrew Wallace, Ph.D, Queens University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://web.qub.ac.uk/bb/awpage/wallace.html From owner-repertoires@net.bio.net Wed May 21 23:00:00 1997 Path: biosci!biosci!not-for-mail From: David Kristofferson Newsgroups: bionet.molecules.repertoires Subject: IMPORTANT - BIOSCI moving to Stanford! Date: 22 May 1997 01:54:45 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 58 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <199705220406.VAA04856@net.bio.net> NNTP-Posting-Host: net.bio.net After more than a decade of serving the biology community on the BIOSCI project, I have decided that it is time for me to pass the torch. The demands of my career and family are making it increasingly difficult for me to run BIOSCI adequately on a part-time basis, and I've decided that I would do the project more harm than good by clinging to it. Therefore I have concluded an agreement with the Stanford University Libraries to take over the management of the BIOSCI project effective 6 June 1997. Many of you know the work of the Stanford Libraries through HighWire Press which has done an excellent job bringing the Journal of Biological Chemistry, Science Magazine, and other prestigious journals to the WWW (see http://highwire.stanford.edu/). I have every confidence that Stanford will provide an environment where the BIOSCI project can flourish. The BIOSCI team at Daresbury will continue to support the project in Europe, and Dave Mack and Julie Lawrence on the current BIOSCI staff will continue to assist the new team at Stanford. The project will be managed by Serge Taylor of the Stanford Libraries who is responsible for developing Web-based "knowledge environments" for scientists. I will still participate with the project in an advisory capacity but have to phase myself out of day-to-day operations. Many of you who read bionet.announce, bionet.biology.computational, and bionet.jobs.offered (the groups that I moderate) have been aware of the recent posting delays that my busy schedule has inflicted on those newsgroups. I am pleased to announce that Todd McGee, Scientific Advisor of the HighWire Press, will be taking over moderation of those groups from me this week. ** NOTE ** - The BIOSCI hardware will be down for backups on 6 June and will be moved over the weekend of June 7 and 8. We will be changing the IP number of the machine, but the host name and all mailing addresses will remain unchanged. There may be a few days of service disruption during this time due to the move unfortunately, just as we experienced in BIOSCI's previous move. We hope to keep this to a minimum. We will post status reports on the move to bionet.announce before and after it happens. Please watch that newsgroup for details. It has been a pleasure serving all of you since the "early days" of biology on the Internet. I've had the pleasure of not only "knowing" many of you over the Internet, but have also met literally thousands of biologists around the U.S. and elsewhere during the many Internet training seminars that I gave over the last decade. I've been truly fortunate to have been given the chance to play a role in the growth of biology on the Internet. This is something I will always treasure. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-repertoires@net.bio.net Tue May 27 23:00:00 1997 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.molecules.repertoires Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 28 May 1997 02:05:26 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 242 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <199705280900.CAA16286@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-repertoires@net.bio.net Tue May 27 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Dieter Poppinger Newsgroups: bionet.molecules.repertoires Subject: open position Date: 28 May 1997 02:03:14 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5mgrgn$fj1@mserv1.dl.ac.uk> Reply-To: Dieter Poppinger NNTP-Posting-Host: net.bio.net Novartis Crop Protection is offering a postgraduate position in scientific software development. Required: background in chemistry / biochemistry, experience in Unix and C. Knowledge of database systems and internet technology desirable. Language: German and/or English. To work of scientific information and data analysis systems, with emphasis on tools for combinatorial chemistry and web-based applications. Open immediately. Contact: Dr. Dieter Poppinger (dpoppi@chbs.ciba.com) Research Computing Novartis Crop Protection AG CH-4002 Basel, Switzerland From owner-repertoires@net.bio.net Tue May 27 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Jim Wade Newsgroups: bionet.molecules.repertoires Subject: Industrial PostDoc Position Date: 28 May 1997 01:59:48 -0700 Organization: Hercules, Inc. Lines: 50 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <338B3F44.2EA5@herc.com> NNTP-Posting-Host: net.bio.net INDUSTRIAL POST-DOCTORAL POSITION Hercules Incorporated, a world leader in water-soluble polymers, paper chemicals, resins, food gums, and non-woven fibers, is seeking one postdoctoral associate to join our Corporate Technology team. This group is engaged in developing new bioconversion technologies, and the person selected would play a key role in developing new high-throughput assay systems. A Ph.D. in chemistry or biochemistry and relevant bioassay experience is required. Responsibilities include development of rapid assay methods to screen for novel enzyme activities, new methods to follow the structural and rheological changes which occur during enzymatic modification of complex carbohydrates, and development of replacement, high-throughput methods for traditional wet chemical assays. The primary emphasis will be on developing new assays for the discovery and lead development phase of research. A strong background in bioassay methods, molecular probes, carbohydrate chemistry, and enzyme reactions is essential. Familiarity with spectroscopic techniques, polymer physical chemistry, and rheological characterization is highly desirable. Duration of the position is up to two (2) years maximum and is available immediately. The location of the Hercules Research Center is suburban Wilmington, Delaware. Please send or FAX resume to: Human Resources Department Hercules, Incorporated Research Center 500 Hercules Rd. Wilmington, DE 19808-1599 FAX: 302/995-4164 Hercules, Incorporated is an Equal Opportunity Employer. No candidate will be discriminated against on the basis of race, sex, national origin, age, color, religion, veteran status, disability, or any other protected class. From owner-repertoires@net.bio.net Tue May 27 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Updated conferences including Cambridge RSC meeting Date: 28 May 1997 10:27:11 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 177 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5mhm8q$ml7@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net Meetings and courses on combinatorial libraries and phage display. ***************************************************************** _________________________________________________________________ *** MEETINGS *** _________________________________________________________________ MipTec '97: MicroPlate Technologies Hyatt Regency Crystal City Arlington, VA (Washington, DC) June 23-25, 1997 MipTec '97 immediately preceeds The Fourth Annual Conference on High Throughput Screening for Drug Discovery by Cambridge Healthtech Institute, June 25-27, 1997.. Registration for MipTec '97 includes attendance at all conference sessions, receptions, breaks, snack lunches and access to the Tradeshow and Luncheon Seminars. Hotel accomodations are the responsibility of the participant. For fees and further information, please contact: John Devlin, PhD, ARRT International, Inc. PO Box 1838 11 Main St New Milford, CT 06776 USA. e-Mail: arrtintl@prodigy.net _________________________________________________________________ Cambridge Healthtech Institute Fourth Annual HIGH-THROUGHPUT SCREENING June 25-27 Arlington, Virginia Cambridge Healthtech Institute 1037 Chestnut Street Newton Upper Falls, MA 02164 USA Phone: 617-630-1300 Fax: 617-630-1325 e-mail: chi@healthtech.com http://www.healthtech.com/conferences/ _________________________________________________________________ NEW*NEW*NEW*NEW*NEW*NEW*NEW* NEW *NEW*NEW*NEW*NEW*NEW*NEW*NEW*NEW Combinatorial Approaches to Chemistry and Biology 27-30 July 1997 Churchill College, Cambridge, UK Organised by the Royal Society of Chemistry Closing date for applications 13 June 1997 Contact: Elaine Wellingham Conference Secretariat Field End House Bude Close Nailsea Bristol BS19 2FQ UK Tel. & Fax: +44 (0)1275 853311 Email: confsec@dial.pipex.com _________________________________________________________________ Cambridge Healthtech Institutes First European Conference on Combinatorial Chemistry and High Throughput Screening December 4-5, 1997 Hotel Arts Barcelona Barcelona, Spain Contact: Irene Phelan, Conference Director Phone: 617-630-1362 Fax: 617-630-1325 e-mail: iphelan@healthtech.com Organized by Cambridge Healthtech Institute 1037 Chestnut Steet, Newton Upper Falls, MA 02164 USA http://www/healthtech.com/conferences/ _________________________________________________________________ IBC's 2nd Annual Conference on Display Technologies: Advances in Protein Engineering, Drug/Vaccine Development and Molecular Evolution; August 4-5, 1997-Boston, MA For More Information: Call IBC USA Conferences at 508-481-6400, Fax: 508-481-4473 or email aheghinian@ibcusa.com for complete conference brochure or Register Online at http://www.ibcusa.com/conf/display _________________________________________________________________ Phage Display of Proteins and Peptides Biochemical Society Meeting University College Galway 3-5 September 1997 Deadline for poster registration 6 June 1997 Further information from: Michelle Mandale Meetings Office The Biochemical Society 59 Portland Place London W1N 3AJ Fax: (+44)171-637-7626 email: meetings@biochemsoc.org.uk __________________________________________________________________ The 8th Cyprus Conference on New Methods in Drug Research Limassol, Cyprus May 4-9, 1998 Conference Executive: Chair: Alex Makriyannis (U. Conn. Storrs, USA) Committee: N. Castagnoli (USA), J.P. Devlin (USA), U. Hacksell (Sweden), L.H. Hurley (USA) and M. Abou-Gharbia (USA) Conference Location Time and Registration: The Conference will take place from Monday, May 4th through Friday, May 9th 1998 at the five-star Hawaii Beach Hotel, Limassol, Cyprus. Attendance is restricted to 150 participants to encourage interaction. Registration fees ($595 per person; $395 for academics) includes attendance at all sessions and morning and afternoon breaks, but not meals. Sponsor: The European Federation of Medicinal Chemistry Contact: ARRT International, Inc., P.O. Box 1838, New Milford, CT 06776, USA Telephone: 860-355-5195; Facsimile: 860-355-5975; e-Mail: arrtintl@prodigy.net _________________________________________________________________ *** COURSES *** _________________________________________________________________ Carolina Workshop on Combinatorial Molecules June 22 - 30, 1997 Theory and Applications of Combinatorial Libraries for Molecular Recognition Studies and Identification of Antagonists Tuition: $1200. Full consideration will be given to applications received by May 9, 1997. To Apply: Send a brief letter describing how this workshop will further your research project(s), along with your CV to the workshop coordinator: Wayne Litaker Program in Molecular Biology and Biotechnology University of North Carolina at Chapel Hill 442 Taylor Hall, CB # 7100 Chapel Hill, NC 27599-7100 office: (+1)919-929-0067 Fax: (+1)919-966-6821 email: litaker@med.unc.edu For additional information contact: briankay@email.unc.edu OR http://fordham25.bio.unc.edu/workshop.html __________________________________________________________________ PHAGE DISPLAY OF COMBINATORIAL ANTIBODY LIBRARIES Cold Spring Harbor Laboratory November 4 -17 , 1997 APPLICATION DEADLINE: July 15, 1997 Organisers: Carlos Barbas, Scripps Research Institute Dennis Burton, Scripps Research Institute Gregg Silverman, University of California, San Diego For Additional Information, please contact: Cold Spring Harbor Laboratory The Meetings & Courses Office 1 Bungtown Road, PO Box 100 Cold Spring Harbor, NY 11724-2213 USA Phone: (+1)516-367-8346 Fax: (+1)516-367-8845 email: meetings@cshl.org __________________________________________________________________ ------------------------------------------------------------------ Andrew Wallace, Ph.D, Queens University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://web.qub.ac.uk/bb/awpage/wallace.html From owner-repertoires@net.bio.net Wed May 28 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Andrew Wallace Newsgroups: bionet.molecules.repertoires Subject: Re: Screening random peptide libraries with polyclonal antibodies Date: 29 May 1997 02:09:57 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 61 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5mjgsm$4ri@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net On Thu, 29 May 1997 09:42:11 +0100 Daniel Lazard wrote: > I would like to know if it is possible to screen a random peptide library > with antibodies derived from polyclonal sera. I realize this has been done > many times with monoclonals but I suspect it is much more difficult to do > with polyclonal Abs. > More specifically, I would like to screen such a library with > autoantibodies isolated from the sera of patients suffering from > autoimmune diseases. The autoantibodies are against a defined autoantigen > and could presumably be purified to a reasonable degree of purity (but > what is "reasonable" for my purposes?). > Do you feel that this a feasable project or not? > Any answers and advice by e-mail will be very much appreciated. > > -- > Dr. Daniel Lazard Tel: 03-9376280 > Felsenstein Medical Research Center Fax: 03-9211478 > Beilinson Campus, Petah-Tikva 49100 e-mail: dlazard@netvision.net.il > Israel Daniel, The kind of approach you describe can indeed be done with patient sera and has been carried out for a number of autoimmune and other diseases. Rheumatoid Arthritis Dybwad, A. et al., (1993) Eur. J. Immunol. 23: 3189-3193. Hepatitis B Folgori, A. et al., (1994) EMBO Journal 13: 2236-2243. Type-1 Diabetes Mennuni, C. et al., (1996) J. Autoimmunity 9: 431-436. Multiple Schlerosis Cortese, I., et al., (1996) Proc. Natl. Acad. Sci. USA 93: 11063-11067. Wegener's granulomatosis Finnern, R. et al., (1997) Clin. Exper. Immunol. 107: 269-281. These are just a few examples, there are probably several others if you search carefully. All of the above cases involved screening for polyclonal antibodies from patient sera or CSF using phage displayed peptide libraries. I doubt if any purification of the patient antibodies is necessary, though I'm sure it would help if it can be done correctly. Comments from those of you on this newsgroup who have performed this work? Andrew ------------------------------------------------------------------ Andrew Wallace, Ph.D, Queens University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://web.qub.ac.uk/bb/awpage/wallace.html From owner-repertoires@net.bio.net Wed May 28 23:00:00 1997 Path: biosci!biosci!not-for-mail From: dlazard@netvision.net.il (Daniel Lazard) Newsgroups: bionet.molecules.repertoires Subject: Screening random peptide libraries with polyclonal antibodies Date: 29 May 1997 00:47:21 -0700 Organization: NetVision LTD. Lines: 20 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I would like to know if it is possible to screen a random peptide library with antibodies derived from polyclonal sera. I realize this has been done many times with monoclonals but I suspect it is much more difficult to do with polyclonal Abs. More specifically, I would like to screen such a library with autoantibodies isolated from the sera of patients suffering from autoimmune diseases. The autoantibodies are against a defined autoantigen and could presumably be purified to a reasonable degree of purity (but what is "reasonable" for my purposes?). Do you feel that this a feasable project or not? Any answers and advice by e-mail will be very much appreciated. -- Dr. Daniel Lazard Tel: 03-9376280 Felsenstein Medical Research Center Fax: 03-9211478 Beilinson Campus, Petah-Tikva 49100 e-mail: dlazard@netvision.net.il Israel From owner-repertoires@net.bio.net Wed May 28 23:00:00 1997 Path: biosci!biosci!not-for-mail From: NLJ Newsgroups: bionet.molecules.repertoires Subject: Position open as organic chemist within automated chemistry. Date: 29 May 1997 11:54:44 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 50 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5mk8i3$6qb@mserv1.dl.ac.uk> Reply-To: NLJ NNTP-Posting-Host: net.bio.net Organic Chemist Novo Nordisk is in the process of establishing automated organic synthesis as an integrated part of its medicinal chemistry research. In order to reinforce this activity with regard to the creation of synthetic compound libraries we are seeking a specialist in organic chemistry. We offer: - A flexible research environment where responsibility matches the assigned tasks. - A youthful and energetic research team. - Modern well-equipped laboratories. - Many unsolved scientific problems and major challenges. - Salary according to qualifications. Qualifications: - A thorough knowledge and broad experience within organic chemistry. - Ph.D. level, preferably complemented with experience from a laboratory outside your own country. - Knowledge and experience in the use of computer technology as applied to chemical research and to automation of chemical apparatus. - A desire to work in a multidisciplinary environment where new challenges are presented daily. - Ingenuity and a practical turn of mind. - A sense of humour. - A readiness to solve problems as they arise. Further details concerning the job are available from Jesper Lau tel. +45 4444 8888 ext.34872 or Nils Langeland Johansen tel. +45 4444 8888 ext. 34875. Your application complete with CV and supporting documents and quoting reference no. 7148 should be sent to Personnel Department Health Care, Krogshoejvej 55, Building 9D1.08, DK-2880 Bagsvaerd, Denmark. More information about Novo Nordisk can be obtained at our homepage: http//www.novo.dk From owner-repertoires@net.bio.net Wed May 28 23:00:00 1997 Path: biosci!biosci!not-for-mail From: felici Newsgroups: bionet.molecules.repertoires Subject: Re: Screening random peptide libraries with polyclonal antibodies Date: 29 May 1997 06:26:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 35 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5mjr7d$jdp@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net > I would like to know if it is possible to screen a random peptide library > with antibodies derived from polyclonal sera. I realize this has been doney > many times with monoclonals but I suspect it is much more difficult to do > with polyclonal Abs. > Do you feel that this a feasable project or not? > -- > Dr. Daniel Lazard Daniel, It is a challenging work, the larger the fraction of specific antibodies that are contained in the sera, the less difficul will be to select specific clones. You can find detailed protocols in: Felici et al. Methods in Enzymology (1996) 267:116-129 Please see also: Folgori et al. EMBO J. (1994) 13:2236-2243 Prezzi et al. J.Immunol. (1996) 156:4504--4513 Mecchia et al. J Immunol. (1996) 157: 3727-3736 Cortese et al. Proc. Natl. Acad. Sci. U.S.A. (1996) 93:11063-11067 Mennuni et al. J. Autoimmunity (1996) 9:431-436 Mennuni et al. J. Mol. Biol. (1997) 268:599-606 Best wishes, Franco Felici --------------------------------- Franco Felici, Ph.D. Department of Biology University of Rome "Tor Vergata" via della Ricerca Scientifica 00173 Rome - Italy e-mail: felici@utovrm.it Fax: +39 6 202 3500