From owner-repertoires@net.bio.net Sun Jun 01 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Wendy Warr Newsgroups: bionet.molecules.repertoires Subject: CSA Trust award Date: 2 Jun 1997 01:33:04 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 76 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5mu02u$2uj@mserv1.dl.ac.uk> Reply-To: Wendy Warr NNTP-Posting-Host: net.bio.net This is being posted to multiple lists, with apologies for duplication. CHEMICAL STRUCTURE ASSOCIATION TRUST 1997 AWARD The Chemical Structure Association Trust is an internationally recognised Trust established to promote education, research or development in the area of systems and methods for the storage, processing and retrieval of information about chemical structures, reactions and compounds. Applications are invited for the 1997 Chemical Structure Association Trust Award. The Trust is offering an Award of two thousand pounds sterling for the best applicant seeking funds for education or research in chemical information. Anyone working in the field of chemical information research can apply and application can be made for funds to attend a relevant conference, for travel (e.g. to collaborate with another research group) or for hardware or software to assist with the research project. The application should include: 1. A statement of academic qualifications. 2. Details of relevant work and a statement of research recently completed by the applicant. 3. The purpose for which the award is required. The clarity and relevance of this statement will be crucial in evaluating the applications. 4. Letters from two academic referees to support the application. The Trust has previously supported the continuation of research studies in biomedical interactions including molecular recognition processes and drug design; a novel combination of reaction indexing and synthesis planning; clustering of chemical structures for property prediction; and investigation of reaction mechanisms. The work of younger scientists in developing countries has also been made possible in conjunction with some of the awards. Recent award winners, and their areas of research, are as follows: - Weifan Zheng, University of North Carolina (QSAR and combinatorial chemistry) - Aniko Simon, University of Leeds (chemical literature data extraction) - Eugene Babaev, Moscow State University (computer-assisted synthesis) - Gareth Jones, University of Sheffield (for presentation of a paper on genetic algorithms at an ACS National Meeting) - Vladimir Kvasnicak, Slovak Technical University (neural networks for prediction of physiochemical properties) - Rainer Herges, University of Erlangen-Nuernberg (reaction databases and quantum chemistry) - Dmitry Lushnikov and Vladimir Shcherbukhin, Zelinski Insitute, Moscow (reaction mechanisms and heterocyclic ring transformations) Applications must be submitted by 31st July 1997, preferably by e-mail, to Professor Michael Lynch at: Michael F Lynch Any postal applications should be sent to: Professor M.F. Lynch Chairman of CSA Trust Awards Sub-Committee Rural Route #1 Avonport Kings County Nova Scotia B0P 1B0 Canada The Award will be presented this year at the International Chemical Information Meeting in Nimes in October. Dr Wendy A Warr Wendy Warr and Associates, 6 Berwick Court Holmes Chapel, Cheshire CW4 7HZ, England Tel/fax +44 (0)1477 533837 wendy@warr.com http://www.warr.com From owner-repertoires@net.bio.net Sun Jun 01 23:00:00 1997 Path: biosci!biosci!not-for-mail From: fennema@chem.rug.nl (Marko) Newsgroups: bionet.molecules.repertoires Subject: electrophoresis of phages on agarose gels? Date: 2 Jun 1997 07:09:42 -0700 Organization: RUG Lines: 19 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5muiv5$k3m@info.service.rug.nl> Reply-To: fennema@chem.rug.nl NNTP-Posting-Host: net.bio.net Hello, I have a short question on running phages (filamentous) in agarose gels. Do they keep their protein coat when run in 1% agarose, can I preform westernblots when blotted to NC and is it possible to isolate infectious particles, (e.g. ATU determination of a gel slice)? Thanks, Marko P.S. Hopefully postings like these give somewhat more life to this newsgroup..... P.P.S. My thanks to the Janet Clench postings they are really helpfull. From owner-repertoires@net.bio.net Mon Jun 02 23:00:00 1997 Path: biosci!biosci!not-for-mail From: rakonj@rockvax.rockefeller.edu (Jasna Rakonjac) Newsgroups: bionet.molecules.repertoires Subject: electrophoresis of phage on agarose gel Date: 3 Jun 1997 10:05:48 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 49 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net >Hello, > >I have a short question on running phages (filamentous) in agarose gels. >Do they keep their protein coat when run in 1% agarose, can I preform >westernblots when blotted to NC and is it possible to isolate infectious >particles, (e.g. ATU determination of a gel slice)? > >Thanks, > >Marko > > Hi, Marko, Here are the references where you will find some of the information you need: For agarose electrophoresis of the native phage virions: Crissman, J.W. and Smith, G.P., 1984, Virology 132, 445-455. For titering the phage from the agarose gel slices: Lopez, J and Webster, R. E., 1983, Virology, 127, 177-193. It is not clear whether you want to transfer native virions to the NC filters, or first disassemble virion in situ and then do the transfer. My guess is, if you make a thin gel, say in a vertical protein gel apparatus, pre-disassemble virions in the gel (as described in Crissman and Smith, 1984) and add SDS to the transfer buffer, proteins should transfer to the membranes. Regards, Jasna Jasna Rakonjac Zinder-Model Laboratory The Rockefeller University 1230 York Avenue, Box 24 New York, NY 10021 U.S.A. Phone: 212-327-8649 Fax: 212-327-7850 From owner-repertoires@net.bio.net Tue Jun 03 23:00:00 1997 Path: biosci!biosci!not-for-mail From: o.h.brekke@bio.uio.no (Ole Henrik Brekke) Newsgroups: bionet.molecules.repertoires Subject: Post Doc position in Norway Date: 4 Jun 1997 07:04:42 -0700 Organization: Universitet i Oslo Lines: 19 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5n3ruc$2hd$2@ratatosk.uio.no> NNTP-Posting-Host: net.bio.net UNIVERSITY OF OSLO Postdoc position in Immunology/Cell biology (up to 3 yrs) EC-TMR Postdoc position for EC citizen and associated states. Participation in TMR Research Networks: "Intracellular mechanisms of antigen processing and presentation by the MHC Class I and Class II molecules" and "Sorting of endosomal and lysosomal proteins by molecular machineries" ERBFMRXCT960069 og ERBFMRXCT960058 (http://www.cordis.lu/tmr/home.html). Possible research visit to several collaborating laboratories. Salary: aprox. 3000 ECU/mth Only citizens of EC member state + Iceland and Israel. Further info and applic.: Dr. Oddmund Bakke, Dep. of Molecular Cell Biology, Box 1050, University of Oslo, N- 0316 Oslo. email: obakke@bio.uio.no. Phone +47 22855787 fax: +47 22854605 From owner-repertoires@net.bio.net Tue Jun 03 23:00:00 1997 Path: biosci!biosci!not-for-mail From: mjablins@rglandes.com (Maureen Jablinske) Newsgroups: bionet.molecules.repertoires Subject: Landes Bioscience, Intracellular Antibodies Book Date: 4 Jun 1997 07:38:29 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 42 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5n3udh$mkf@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net Intracellular Antibodies: Developments and Applications, Antonino Cattaneo & Silvia Biocca (eds), Landes Bioscience, Biotechnology Intelligence Unit (BIU) series, 1997 Distributed worldwide by Springer Verlag A description of the Cattaneo/Biocca book follows: "Recent advances in the field of recombinant antibodies have permitted the manipulation of genes coding for specific antibodies, thus allowing their ectopic expression in a wide variety of non-lymphoid cells. This volume describes how the ectopic expression of antibodies, as secreted or as intracellularly retargeted molecules, can be exploited to block biological functions or to confer new phenotypic traits (e.g. resistance to a virus). The authors became involved very early on in the development and application of the technology. This is the first book describing this emerging technology, which is receiving increasing attention for application in many different fields and biological systems--from human gene therapy to plant biotechnology. Also, this is the first book to cover the principles and the applications of the use of ectopic antibody expression for intracellular and intercellular immunization. Since the technology is based on concepts from different areas--such as the cell biology of intracellular trafficking, the engineering and folding of new antibody forms, the biology of specific systems of interest, and so on--there exists a need to describe the general principles and ideas, as well as to review what has already been done. This book aims to put interested readers in the position of being able to critically understand and use the technology of intracellular antibodies for their own interests." In addition to our Biotechnology series, we have a Molecular Biology series, as well as others which may be of interest to the newsgroup. (Inquiries/orders may be sent to me at mjablins@rglandes or orders@rglandes.com) Maureen Jablinske, Biotechnology Series Editor From owner-repertoires@net.bio.net Tue Jun 03 23:00:00 1997 Path: biosci!biosci!not-for-mail From: o.h.brekke@bio.uio.no (Ole Henrik Brekke) Newsgroups: bionet.molecules.repertoires Subject: Post doc position Date: 4 Jun 1997 07:09:15 -0700 Organization: Universitet i Oslo Lines: 22 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5n3sa8$2ie$2@ratatosk.uio.no> NNTP-Posting-Host: net.bio.net [Reposting due to error - apologies for any duplication. AW] UNIVERSITY OF OSLO Postdoc position in Immunology/Cell biology (up to 3 yrs) EC-TMR Postdoc position for EC citizen and associated states. Participation in TMR Research Networks: "Intracellular mechanisms of antigen processing and presentation by the MHC Class I and Class II molecules" and "Sorting of endosomal and lysosomal proteins by molecular machineries" ERBFMRXCT960069 og ERBFMRXCT960058 (http://www.cordis.lu/tmr/home.html). Possible research visit to several collaborating laboratories. Salary: aprox. 3000 ECU/mth Only citizens of EC member state + Iceland and Israel. Further info and applic.: Dr. Oddmund Bakke, Dep. of Molecular Cell Biology, Box 1050, University of Oslo, N- 0316 Oslo. email: obakke@bio.uio.no. Phone +47 22855787 fax: +47 22854605 From owner-repertoires@net.bio.net Wed Jun 04 23:00:00 1997 Path: biosci!biosci!not-for-mail From: toshi@alumnae.caltech.edu (Toshi Takeuchi) Newsgroups: bionet.molecules.repertoires Subject: Re: electrophoresis of phages on agarose gels? Date: 5 Jun 1997 03:01:43 -0700 Organization: Caltech Alumni Association Lines: 23 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5n4h0m$t7e@gap.cco.caltech.edu> References: <5muiv5$k3m@info.service.rug.nl> NNTP-Posting-Host: net.bio.net In article <5muiv5$k3m@info.service.rug.nl>, Marko wrote: >I have a short question on running phages (filamentous) in agarose gels. >Do they keep their protein coat when run in 1% agarose, can I preform >westernblots when blotted to NC and is it possible to isolate infectious >particles, (e.g. ATU determination of a gel slice)? Hello Marko. I have done westerns from phage in agarose gels. I used antibodies against a protein that was displayed on the phage. I pour the agarose gel as normal, except the buffer I use is: (5x buffer is: 1.25M glycine, 0.125M Tris, pH 8.6) I run 4mA for 14-16 hours. I run the western as normal, except I let it run a little longer, since the agarose is fairly thick. Toshi From owner-repertoires@net.bio.net Thu Jun 05 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "D. KIM" Newsgroups: bionet.molecules.repertoires Subject: Re: electrophoresis of phages on agarose gels? Date: 6 Jun 1997 02:30:08 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 39 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net On 4 Jun 1997, Toshi Takeuchi wrote: > In article <5muiv5$k3m@info.service.rug.nl>, Marko wrote: > > Hello Marko. I have done westerns from phage in agarose gels. > I used antibodies against a protein that was displayed on the > phage. > > I pour the agarose gel as normal, except the buffer I use is: > > (5x buffer is: 1.25M glycine, 0.125M Tris, pH 8.6) > > I run 4mA for 14-16 hours. I run the western as normal, except > I let it run a little longer, since the agarose is fairly thick. > > Toshi Do you run the gel with Ethidium Bromide? How do you detect the phage band? Will this technique concentrate phage from a crude PEG ppt? I would be interested in agarose gel electrophoresis of phage as a means of concentrating and purifying the phage away from extraneous proteins and nucleic acids from the culture medium. Daniel Kim p.s. I have tried running phage through a 1% agarose TAE gel with ethidium bromide (just R408 helper phage) and detect two bands which migrate VERY slowly. Why would I get two bands? I have not tried to extract and titer them. Could I recover the phage by a freeze/squeeze method? I'll have to try this out sometime soon. D.K. From owner-repertoires@net.bio.net Tue Jun 10 23:00:00 1997 Path: biosci!biosci!not-for-mail From: John McCafferty Newsgroups: bionet.molecules.repertoires Subject: Programme of Galway Phage Display Meeting Date: 11 Jun 1997 09:14:24 -0700 Organization: CAT Lines: 88 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <339C19DE.3C19@camb-antibody.co.uk> Reply-To: john.mccafferty@camb-antibody.co.uk NNTP-Posting-Host: net.bio.net PHAGE DISPLAY OF PROTEINS AND PEPTIDES. 3-4th September 1997 Joint meeting of Protein and Peptide Science Group of The Biochemical Society and The EU Phage Club ---------------------------------------------------------------------- 3rd September 9.00 John McCafferty (Cambridge, UK) Introductory remarks 9.05 Philippe Holliger (Cambridge, UK) Gene 3 protein and phage infection. 9.45 Stefania Spada (Zurich, Switzerland) Selectively Infective Phages. 10.30 Coffee 11.00 Andrew Bradbury (Trieste, Italy) Towards selection by infection. Augmenting the host range of phage fd ANTIBODY-PHAGE 11.45 Ian Tomlinson (Cambridge, UK) Reproducing human antibody diversity in a test tube 12.30 p.m. Lunch 1.30 p.m. Soeren Buus (Copenhagen, Denmark) Selection of antibodies with antigen specific MHC restricted specificity of T cells 2.15 p.m. Jane Osbourn (Cambridge, UK) Ligand directed cell surface selections 3.00 p.m. Coffee/Posters 4.00 p.m. Laurent Jespers (Leuven, Belgium) Functional epitope mapping by negative selection of phage displayed randomised protein libraries 4.45 p.m. Short Presentations from selected posters 4th Sepember 1997 PEPTIDE AND PROTEIN-PHAGE 2.00 p.m. Brian Kay (North Carolina, USA) Defining the specificity of SH3 domains with random peptide libraries 2.45 p.m Nick Wrighton (Affymax, USA) Peptide Mimetics of Erythropoietin 3.30 p.m. Coffee ALTERNATIVE DISPLAY TECHNOLOGIES 4.00 p.m. Ian Jones (Oxford, UK) Baculovirus display 4.45 p.m. Mark Chadwick (Cambridge, UK) Retroviral Display ---------------------------------------------------------------------- This symposium will form part of The Biochemical Society Meeting (3-5th September 1997). There will be a poster session associated with this symposium. Poster registration forms and further information on the meeting are available from: The Meetings Office, The Biochemical Society, 59 Portland Place, London W1N 3AJ Fax 0171 637 7626 e.mail meetings@biochemsoc.org.uk http://www.biochemsoc.org.uk/meetings/galway97/default.htm ----------------------------------------------- John McCafferty, Cambridge Antibody Technology, The Science Park, Melbourn, Cambs SG8 6JJ Phone : 01763 263233 (Xt 115) Fax : 01763 263413 e.mail: john.mccafferty@camb-antibody.co.uk --------------------------------------------------------- From owner-repertoires@net.bio.net Thu Jun 12 23:00:00 1997 Path: biosci!biosci!not-for-mail From: takeuchi@cgl.ucsf.edu (Toshi Takeuchi) Newsgroups: bionet.molecules.repertoires Subject: Re: electrophoresis of phages on agarose gels? Date: 13 Jun 1997 00:54:32 -0700 Organization: Caltech Alumni Association Lines: 30 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5na6mo$21a@gap.cco.caltech.edu> References: NNTP-Posting-Host: net.bio.net In article , D. KIM wrote: >Do you run the gel with Ethidium Bromide? How do you detect the phage >band? Will this technique concentrate phage from a crude PEG ppt? >I would be interested in agarose gel electrophoresis of phage as a means >of concentrating and purifying the phage away from extraneous proteins and >nucleic acids from the culture medium. I usually run two lanes and cut the gel in half. I one half I run the Western, but on the other half I soak the gel in 0.5M NaOH for 4 hours (I assume to denature the protein coat), and then soak in water with several changes over 1hr, and then stain with ethidium. If there is too much NaOH, you will not be able to stain and you will need to wash for longer. I thought I tried to stain with ethidium directly, and it didn't work, but I don't recall anymore. If that works, then it would save me time :). You can definitely separate phagemid phage from helper phage because of the size difference. It would certainly separate the phage DNA from the rest of the DNA/proteins, but I don't know about getting infective phage out of the agarose. However, if you are working with phagemids, then you can simply retransform with the appropriate DNA band. I only see one band with helper phage (VCSM13), and one band for my phagemid phage. Toshi From owner-repertoires@net.bio.net Thu Jun 12 23:00:00 1997 Path: biosci!biosci!not-for-mail From: SciCentral Newsgroups: bionet.molecules.repertoires Subject: BEST BIOCHEMISTRY DIRECTORIES Date: 13 Jun 1997 09:26:23 -0700 Organization: SciLink Lines: 38 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5nrsbf$2ve@net.bio.net> Reply-To: scicentral@scicentral.com NNTP-Posting-Host: net.bio.net Dear Colleagues, We invite you to attend an opening in your honor at: http://www.scicentral.com This unique Web site has been created for scientists and engineers by scientists who know your needs. Just three clicks will get you everywhere. http://www.scicentral.com includes only the most valuable directories on the Web. They currently constitute a gateway to over 50,000 scientific sites pertaining to over 120 specialties in science and engineering ranging from health, biological, earth, physical, and engineering sciences, to government and institutional listings, and including the Commerce Business Daily and Medline--all free. We envision http://www.scicentral.com as the science and engineering hub of the World Wide Web, a place for professionals to gather and discuss the complex issues facing us all as we approach the 21st century. This is where you can make your opinions known. We plan editorials, chat rooms, news. We will continue to be responsive to your suggestions. Please visit http://www.scicentral.com often and bring your colleagues. Ellen S. Uffen, Ph.D. President, SciLink, Inc. Guy Orgambide, Ph.D. Chief Executive Officer SciLink, Inc. Robert L. Uffen, Ph.D Professor Emeritus Michigan State University From owner-repertoires@net.bio.net Mon Jun 16 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Itai Benhar Newsgroups: bionet.molecules.repertoires Subject: scFv insert instability Date: 17 Jun 1997 06:20:03 -0700 Organization: Dept. Molec. Microbiol&Biotech, Tel-Aviv, Tel Aviv Israel Lines: 29 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <33A6940F.42D4@ccsg.tau.ac.il> Reply-To: benhar@ccsg.tau.ac.il NNTP-Posting-Host: net.bio.net Hello to all antibody-phageopilh I would like to discuss a problem encountered by many researchers using the Pharmacia Recombinant Phage Antibody System; instability of cloned inserts. This phenomenon plagues experiments where multiple panning rounds are required to obtain a rare binder from naive repertoires. Our observations are that prolonged culture of phagemid-containing bacteria, even without going through helper-phage rescue, will result in substential insert-loss (partial or complete). Growing at 25 or 30 degrees is no better then at 37. Thus, libraries in such vectors are severely biased for "E. coli friendly" sequences, which is evident as affinity-isolated phage clones are much less prone to insert loss. Recent developments in Andreas Pluckthuns lab resulted in phage-display vectors where insert expression is tightly regulated and are more stabely maintained (Krebber et al J. Imm. Methods 201:35-55 1997, and reference therein). Does anyone have hands-on experience with such vectors where the issue of insert stability during 3-5 panning rounds was addressed? Comments anyone? Itai Benhar, Ph.D. Dept Molecular Microbiology & Biotechnology Tel-Aviv University, Israel benhar@post.tau.ac.il From owner-repertoires@net.bio.net Tue Jun 17 23:00:00 1997 Path: biosci!biosci!not-for-mail From: paimm@pop.netgate.net Newsgroups: bionet.molecules.repertoires Subject: Symposium Announcement Date: 18 Jun 1997 04:51:05 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 67 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <199706171850.LAA04629@ng.netgate.net> NNTP-Posting-Host: net.bio.net ****Symposium Announcement**** Therapeutic Antibody Technology 97 PLACE/DATE: Holiday Inn--Union Square, San Francisco, CA; Sept. 21-24, 1997. Antibodies are a promising form of biotherapy. Four advances have made possible the facile generation of recombinant therapeutic monoclonal antibodies: PCR, bacterial expression, phage display and combinatorial methods. Pioneers in each of these fields will present their latest work. Some monoclonal antibodies have been successfully tested in clinical trials and this progress will be presented. Many of the world leaders in these technologies and their application will discuss recent work in this important and rapidly changing field. Keynote Address: Richard A. Lerner, Scripps Research Institute Confirmed Speakers: Carlos Barbas Scripps Carl Borrebaeck Sweden Dennis Burton Scripps Peter Colman Australia Brian Dallaire IDEC Zelig Eshhar Israel Neil Greenspan Case Western Andrew Griffiths Cambridge, UK R. Houghten Torrey Pines H. Hoogenboom Netherlands Peter Hudson CSIRO Aya Jakobvits AbGenix Kevin Johnson CAT Richard Junghans Boston Ton Logtenberg Netherlands Wayne Marasco Harvard Roy Mariuzza Maryland James D. Marks UCSF Gordon Moore SKB Sherie Morrison UCLA Achim Knappik Morphosys Paul Parren Scripps Andreas Pl=FCckthun Zurich Michael Pfreudschuh Germany Cary Queen Protein Design John Reno NeoRx Jamie Scott Canada Tom Smith Indiana Anthony Williamson Scripps Ian Wilson Scripps Poster presentations welcome. Organizers: James W. Larrick(1) and Dennis Burton(2) Sponsor: (1)Palo Alto Institute of Molecular Medicine (2)The Scripps Research Institute, La Jolla, CA Information: Palo Alto Institute of Molecular Medicine 2462 Wyandotte Street Mountain View, California 94043, USA TEL: (415)--694-1420; FAX:(415)--694-7717; e-mail: www.pano.com/paimm see also Antibody Resource page http://www.antibodyresource.com/ From owner-repertoires@net.bio.net Tue Jun 17 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Michael Keenan Newsgroups: bionet.molecules.repertoires Subject: Drug Discovery Technology `97 Date: 18 Jun 1997 09:20:48 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 56 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <9705188666.AA866651986@ultra-gw.ibcusa.com> Reply-To: Michael Keenan NNTP-Posting-Host: net.bio.net DRUG DISCOVERY EXPERTS CONVENE THIS AUGUST IN SAN DIEGO FOR GROUNDBREAKING CONFERENCE Full program brochure on-line: http://www.ibcusa.com/conf/drugdisc IBC's 2nd International Exposition and Symposium Drug Discovery Technology `97 Integrating R&D Activities to Accelerate Drug Development August 11-14, 1997 * Sheraton San Diego Hotel and Marina * San Diego, CA * 49 Renowned Speakers * Unpublished Advances * Over 75 Exhibitors * Two Parallel Sessions * Scientific Workshops * In-depth Tutorials * Networking Opportunities Several hundred researchers from pharmaceutical and biotechnology companies, universities and government agencies from around the world, will come together at IBC's Drug Discovery Technology `97 conference at the Sheraton San Diego Hotel and Marina on August 11-14, 1997 to discuss the exciting technological advances in this rapidly moving field. "Drug Discovery Technology `97 is a must attend meeting for anyone seeking to create a fast cycle time template for drug discovery in their organization," according to Norton P. Peet, Ph.D., Head, Medicinal Chemistry at Hoechst Marion Roussel. "This conference highlights unpublished data on new methods for target identification and validation, and debuts the latest technologies for lead generation and optimization, which lead to novel therapeutics for the treatment of unmet medical needs" George M. Whitesides, Ph.D., Mallinckrodt Professor of Chemistry at Harvard University, will deliver the Conference Keynote Address on Tuesday, August 12, 1997 and will discuss "Microtechnology and Drug Discovery." Dr. Whitesides was a member of the faculty of the Massachusetts Institute of Technology from 1963 to 1982, and subsequently joined the Department of Chemistry of Harvard University in 1982. A renowned speaker panel of 49 scientists will discuss the latest breakthroughs in genomics, bioinformatics, new target validation, assay technologies, combinatorial chemistry, detection methods, microtechnologies, automation and robotics, information and data management, lead optimization and candidate selection, toxicology as well as other cutting-edge topics. International Business Communications (IBC) is the leading information provider on drug discovery technologies and has set the standard for cutting-edge events in the pharmaceutical and biotechnology field. For more information about IBC or to register for this conference, please contact IBC USA Conferences, 225 Turnpike Road, Southborough, MA 01772-1749, USA. Tel: (508)481-6400 Fax: (508)481-7911 E-mail: inq@ibcusa.com Internet: http://www.ibcusa.com/conf/drugdisc From owner-repertoires@net.bio.net Wed Jun 18 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "Marko Dolinar, JSI" Newsgroups: bionet.molecules.repertoires Subject: 5th Brdo Symposium on Proteinase Inhibitors and Biological Contr Date: 19 Jun 1997 02:27:54 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 68 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <01IK834RMK4I000BKA@CATHY.IJS.SI> NNTP-Posting-Host: net.bio.net Dear Colleagues, The success of the Research Symposia on Proteinase Inhibitors and Biological Control, first organized in 1987 at Brdo, Slovenia, confirmed the need to continue such meetings. The continuing progress and the high level of interest in this field, coupled with the desirability of a European "Gordon Conference style" of meeting, has led us to organize the fifth Symposium in 1997. Topics of the Symposium are: 1. The biological role of proteolysis in: antigen presentation, apoptosis, plants, insects and parasites, carcinogenesis and tumour progression, Alzheimer's disease, viral related diseases 2. Biochemical principles of proteolysis:  Characterization and structure of proteinases and their inhibitors  Insights into function and mechanism revealed by genetic engineering approaches 3. New ideas and prospects. Special attention (one day) will be paid to cysteine proteinases and their protein inhibitors. There will be about 30 - 35 oral presentations given by invited lecturers, and a poster session. The number of participants is limited to 120. On behalf of the Organizers it is our pleasure to invite you to participate in this symposium. Registration fee for the participants is 650 DM which will be used partly to cover your stay during the meeting. The rest of expenses will be covered by the organizers. Contributions of invited speakers and selected posters are considered for publication by an international publishing company similarly to the previous meetings. The 5th Brdo Symposium will be held from October 4 to October 8, 1997 in Kokra Hotel, located at Brdo Estate, Central Slovenia. The meeting is organized by Hans Fritz (Munich, Germany), Bonnie Sloane (Detroit, USA) and Vito Turk (Ljubljana, Slovenia). A homepage of the Symposium is available on Internet. Visit http://bio.ijs.si/conf/brdo97.htm and the links therein pointing to the First announcement, Travel information and On-line registration form. If you have any additional questions about the Symposium or would like to receive the printed version of the Symposium announcement and the registration form, feel free to contact Marko Dolinar from the local organizing committee at the address below. ___________________________________________________ Marko Dolinar Department of Biochemistry and Molecular Biology J. Stefan Institute Jamova 39 SI-1000 Ljubljana, Slovenia :::::::::::::::::::::::::::::::::::::::::::::::: Email> Marko.Dolinar@IJS.SI fax: + 386 61 27 35 94 phone: + 386 61 17 73 900; direct: 17 73 623 or 17 73 306 homepage: http://bio.ijs.si/marko/ department's homepage: http://bio.ijs.si/ From owner-repertoires@net.bio.net Wed Jun 18 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "MarK A. Sullivan" Newsgroups: bionet.molecules.repertoires Subject: Re: scFv Instability Date: 19 Jun 1997 02:19:12 -0700 Organization: Johnson & Johnson Clinical Diagnostics Lines: 30 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <33A83BAE.3D75@cldx.com> NNTP-Posting-Host: net.bio.net Itai Benhar wrote: I would like to discuss a problem encountered by many researchers using the Pharmacia Recombinant Phage Antibody System; instability of cloned inserts. This phenomenon plagues experiments where multiple panning rounds are required to obtain a rare binder from naive repertoires. Our observations are that prolonged culture of phagemid-containing bacteria, even without going through helper-phage rescue, will result in substential insert-loss (partial or complete). Growing at 25 or 30 degrees is no better then at 37. Thus, libraries in such vectors are severely biased for "E. coli friendly" sequences, which is evident as affinity-isolated phage clones are much less prone to insert loss. Recent developments in Andreas Pluckthuns lab resulted in phage-display vectors where insert expression is tightly regulated and are more stabely maintained (Krebber et al J. Imm. Methods 201:35-55 1997, and reference therein). Does anyone have hands-on experience with such vectors where the issue of insert stability during 3-5 panning rounds was addressed? I use a display vector where the gene III fusion protein is expressed off of the alkaline phosphatase promoter. The clones are completely stable in LB medium, do not require any sort of induction when infecting with helper and we observe highly efficient, but probably monovalent display of scFvs or Fabs. For examples, see Malone and Sullivan (1996) J. Mol. Recog. Vol. 9, 738-745. Mark Sullivan Ortho Clinical Diagnostics From owner-repertoires@net.bio.net Sun Jun 22 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Wendy Warr Newsgroups: bionet.molecules.repertoires Subject: Beads et al. Date: 23 Jun 1997 01:07:01 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <9706200807.AA3589@cas.org> Reply-To: Wendy Warr NNTP-Posting-Host: net.bio.net I am doing a market study on solid supports, resins, beads and like materials for combinatorial chemistry. Can anyone tell me if there are already any published reports on this topic? If you are making or selling such products I would also be interested to hear from you. Sorry about duplicate postings.. Wendy Warr Dr Wendy A Warr Wendy Warr and Associates, 6 Berwick Court Holmes Chapel, Cheshire CW4 7HZ, England Tel/fax +44 (0)1477 533837 wendy@warr.com http://www.warr.com From owner-repertoires@net.bio.net Wed Jun 25 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Michael Arlt Newsgroups: bionet.molecules.repertoires Subject: Ellman's THP Linker Date: 26 Jun 1997 02:54:00 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 28 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <199706260801.BAA24302@listserv.arizona.edu> Reply-To: Michael Arlt NNTP-Posting-Host: net.bio.net Hi everybody! We would like to use Ellman's THP Linker. Does anyone know a supplier of (3,4-dihydro-2H-pyran-2-yl)-methanol? It is available from it's corresponding 3,4-dihydro-2H-pyran-2-yl-carboxylic acid ester through reduction. The ester used to be a Fluka product but apparently it is not anymore. If anyone knew a supplier of that stuff it would be great for us, too. It seems that the carboxylic acid ester is accessible via dimerisation of acrolein and subsequent Cannizzarro reaction. Digging in very very old literature (Alder in 1941) revealed that this reaction is a pain, too. Are there maybe more modern procedures out there. Any help is greatly appreciated, Michael --------------------------------------------------------------------------- Dr. Michael Arlt m_arlt@merck.de Merck KGaA Tel. (49) 61 51 72 26 58 Pha Fo Med Chem/CNS FAX (49) 61 51 72 31 29 Frankfurter Str. 250 64271 Darmstadt/Germany Any opinions in this mail do not necessarily match the views of my company! --------------------------------------------------------------------------- From owner-repertoires@net.bio.net Mon Jun 30 23:00:00 1997 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.molecules.repertoires Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 1 Jul 1997 03:14:28 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 236 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <199706280900.CAA04914@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-repertoires@net.bio.net Mon Jun 30 23:00:00 1997 Path: biosci!biosci!not-for-mail From: dprickett@ieo.it (dennis prickett) Newsgroups: bionet.molecules.repertoires Subject: Re: Phage display cDNA systems Date: 1 Jul 1997 03:14:23 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 47 Sender: daemon@net.bio.net Approved: a.wallace@qub.ac.uk Distribution: world Message-ID: <5p5i1p$805@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net Ciao, I refered back to this message and the basic question was not really answered. Are the cDNA phage display libraries from Clontech and AMS Biotechnology useful? Or does one use them with the hope that you just might be fortunate enough to find a binding clone in spite of the previously mentioned problems associated with cloning cDNA fragments into the N-terminus of a phage coat protein? Dennis Prickett >>> Does anyone have experience with the phage display cDNA library system >>> from Clontech? As far as I can tell, their phagemid vector displays the >>> cDNA product at the C-terminus of pIII. How effective is this compared >>> to other display systems such as pVI display or the pJuFo method? >>> >>> Andrew >> >>It seems that I was wrong in my assumption about the construction of the >>display vector. The cDNA library is cloned in at the N-terminus of pIII, >>which makes sense in trying to achieve display, but then the actual >>display of cDNA products will be reduced due to frameshifts and >>missense mutations from introducing the cDNA affecting the expression >>of pIII and therefore functional phage, right? > >Yes, the correction is right. >Your concern was the reason in 1993 to develope pJuFo to be able to: >- clone cDNA directional (still there are three reading frames) >- most importanly poly A does not matter >- several enzymes to cut (but still int 5'- 3'orientation) > >Mark Department of Experimental Oncology European Institute of Oncology Via Ripamonti 435 20141 Milan ITALY ph: +39-2-57489855 FAX: +39-2-57489851 e-mail: dprickett@ieo.cilea.it