From owner-repertoires@net.bio.net Mon Oct 04 21:08:00 1999 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.molecules.repertoires Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 4 Oct 1999 15:07:16 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 242 Sender: daemon@net.bio.net Approved: A.Wallace@Queens-Belfast.AC.UK Distribution: world Message-ID: <199909280900.CAA24238@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 14-AUG-99) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- All BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- the UK-HGMP-Resource Centre (known as hgmp.mrc.ac.uk): ----------------------------------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@hgmp.mrc.ac.uk. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to majordomo@hgmp.mrc.ac.uk. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Please ask for help at biosci@hgmp.mrc.ac.uk if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-repertoires@net.bio.net Mon Oct 04 21:08:00 1999 Path: biosci!biosci!not-for-mail From: Andy Zaayenga Newsgroups: bionet.molecules.repertoires Subject: Announce: Laboratory Robotics Meeting 10/7/99 Date: 4 Oct 1999 15:07:19 -0700 Organization: Netcom Lines: 204 Sender: daemon@net.bio.net Approved: A.Wallace@Queens-Belfast.AC.UK Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net The Laboratory Robotics Interest Group Mid Atlantic Chapter October 1999 Meeting High Throughput Screening: Special Topics Date: Thursday, October 7, 1999 Place: Forsgate Country Club, Forsgate Drive, Jamesburg, NJ 08831 Phone: (732)521-0070 Itinerary: Social Period - 3:30 - 6:00 PM Presentations - 6:15 to 8:30 PM Member Pre-Registration: Requested, not required. Pre-registering will allow us to more accurately gauge seating requirements and refreshment needs. Indicate names of attendees and company affiliation. Pre-register by email with or by phone at (732)302-1038. In order to speed sign-in at the meeting, please bring a business card to drop into the registration box. There will be a business card drawing for one of our beautiful LRIG rosewood pens. ************************************************************* Agenda: The theme of the evening is High-Throughput Screening: Special Topics. After the extended social period from 3:30 to 6:00, the talks will commence leading off with Dr. Sheri Miraglia talking about an exciting new development in scanning laser imaging for high-throughput screening applications. Secondly, we will hear Dr. Susan Bassett talk about the problems of interpreting the voluminous data that we get from HTS and some possible software solutions addressing this problem. Finally, Dr. Dale Christensen will talk about a novel approach in finding small molecules that interact with receptors for which ligands are not necessarily known. Taken together these speakers, who are coming far and wide from California, New Mexico, and North Carolina, will ensure a stimulating evening. As always, there is no registration fee or dues, and food and refreshments will be served throughout the social period. Registration is encouraged for us to accurately gauge the size of the meeting. There are hotels nearby for attendees who wish to stay overnight. There will be a Job posting board at the social. Please encourage your recruiters to give you material to post and distribute. Openings may also be posted at http://www.lab-robotics.org/careers.htm Members interested in presenting a scientific poster are encouraged to do so. Please contact us to arrange for poster space. There is no fee to attend the meeting. ************************************************************* Presentation: High-Throughput Screening Applications of a Novel Scanning Laser Imaging Technology Sheri Miraglia, Ph.D., Senior Scientist, PE Biosystems The increasing number of compounds available for screening in drug development has driven the requirement for higher throughput screening technologies, as well as unique technologies that address a broader application portfolio. Our laboratory has been involved in the development of multiplexed mix-and-read assays using Fluorometric Microvolume Assay Technology (FMAT). FMAT is a fluorescence based assay system that incorporates a laser scanner and optical detection system that provides a direct measurement of cellular or bead-based fluorescence on a well-to-well and on an individual cell/bead basis. This design is ideal for the homogenous identification of hits in primary screening, as well as for lead optimization in the form of IC50 determinations, and for assessment of lead compound cytotoxicity. Fluorescent beads of various sizes can be distinguished from one another, allowing the multiplexing of two or more targets present on different sized beads in the same well. In addition, the digitized image data is compiled from two PMTs permitting the development of multiplexed assays based on dye color. A variety of different mix and read applications for FMAT will be described, including peptide-receptor ligand interactions, and multiplexed bead based immunocapture assays. The results of a novel high-throughput screen performed in collaboration with a major Pharma oncology group will also be discussed. Employing a simple mix-and-read Annexin V binding assay, a variety of purified natural products were identified that are potent inducers of apoptosis in tumor cells. Taken together, the data to be presented will demonstrate the versatility and feasibility of fluorescence-based homogeneous and multiplexed assays for a variety of cell-based and molecular targeted screens used in drug discovery. ************************************************************* Presentation: Harnessing the Power of Computational Intelligence to Identify Leads in HTS Susan I. Bassett, Ph.D., Executive Vice-President, Global Technology Operations, Bioreason, Inc. With high-throughput screening systems in place and beginning to produce data reliably, the data analysis and interpretation becomes a bottleneck in the process of moving more high-quality leads to the clinic. The decision-making processes that go into lead discovery, evaluation, and development are quite complex, and can benefit from judicious use of appropriate computational intelligence techniques. Knowledge-based reasoning systems that capture the decision process of a pharmaceutical chemist during lead identification and development and aid in decision support will be presented in this talk. Bioreason's HTS data interpretation systems are an example of an automated solution aimed at helping identify top quality lead candidates while minimizing costly mistakes. The fundamental aspects of technology for combining computational intelligence techniques with knowledge discovery from data mining to this end will be presented. ************************************************************* Presentation: Molecular Braille: A Novel Technology for Identification and Characterization of Compounds that Modulate Receptor Function Dale J. Christensen, PhD., Senior Scientist Novalon Pharmaceutical Corp., 4222 Emperor Blvd., Suite 560 Durham, NC 27703-8466, Ph 919-474-8888 x34, Fax 919-474-0103, dchristensen@novalon.com Many receptor proteins in cellular signaling pathways undergo significant conformational changes in response to a signal molecule. Nuclear hormone receptors are ligand-dependent transcription factors. Ligand binding to these receptors results in conformational changes that expose binding sites for coactivator or corepresser proteins. The estrogen and androgen receptor are well validated drug targets that have a significant clinical utility while orphan nuclear receptors such as PPAR are beginning to play an important role in modern drug discovery. Molecular Braille has been developed to identify and characterize compounds that modulate the conformation of these receptors. Using a series of conformation-sensitive probes, the conformation that the receptor adopts in response to ligand binding can be evaluated using an in vitro time-resolved fluorescence assay and an in vivo luminescence assay. This technology can be used to identify new ligands, distinguish between classes of ligands, and guide lead optimization. ************************************************************* For more information, contact one of the LRIG Mid Atlantic officers: Dennis S. France Executive Chair, LRIG Home and Mid Atlantic Chapter The Novartis Institute for Biomedical Research tel: (908) 277-5328 fax: (908) 277-4374 email: dennis.france@pharma.novartis.com John Babiak, Ph.D. High Throughput Screening Chair, Mid Atlantic Chapter Pharmacopeia tel: (609) 452-3795 fax: (732) 821-2037 email: jbabiak@pharmacop.com William Haller Analytical Chemistry Chair, Mid Atlantic Chapter Treasurer, LRIG Home and Mid Atlantic Chapter Ortho-McNeil Pharmaceutical tel: (908) 218-6341 fax: (908) 218-0524 email: bill.haller@lab-robotics.org Sharon Reed Event Coordinator, Mid Atlantic Chapter Coelacanth Corporation tel: (609) 448-8200 ext 2044 email: sharon.reed@lab-robotics.org M. Elizabeth Miller Agricultural Applications Chair, Mid Atlantic Chapter Rohm & Haas Company tel: (215) 641-7285 fax: (215) 619-1617 email: rsaumm@rohmhaas.com Jinzi Wu, Ph.D. Assay Development Chair, Mid Atlantic Chapter The Novartis Institute for Biomedical Research email: jinzi.wu@pharma.novartis.com Andy Zaayenga Automation Technologies Chair, Mid Atlantic Chapter Secretary, LRIG Home and Mid Atlantic Chapter TekCel Corporation tel: (732) 302-1038 fax: (732) 302-9080, eFax: (630) 604-2935 email: andy.zaayenga@tekcel.com ************************************************************* Directions: From the North or South: Take the New Jersey Turnpike to Exit 8A - Exit the left ramp for Jamesburg (Route 32 East) - Continue straight for 1 1/4 miles through traffic light - Forsgate Country Club is on your left - Use the Clubhouse Entrance (second left). From Princeton: Route 1 to Scudders Mill Road East - Continue on Scudders Mill Road and make a left at the 5th traffic light onto Dey Road - Continue on Dey Road to the end - Make a left - At 2nd traffic light (Route 32) make a right - Continue straight for 1 1/4 miles through traffic light - Forsgate Country Club is on your left - Use the Clubhouse entrance (second left). ************************************************************* For more information on this meeting, visit http://www.lab-robotics.org/Mid_Atlantic/meetings/9910.htm Visit The Laboratory Robotics Interest Group homepage at http://lab-robotics.org ************************************************************* The Laboratory Robotics Interest Group is a rapidly growing special interest group focused on robotics applications in the laboratory. Our membership consists of over 5,000 scientists and engineers worldwide with more than 2,800 in the Mid Atlantic Chapter. We are a non-profit organization run by unpaid volunteers so if you would like to help out with the group please contact one of our officers. From owner-repertoires@net.bio.net Tue Oct 05 10:09:00 1999 Path: biosci!biosci!not-for-mail From: Amit Galande Newsgroups: bionet.molecules.repertoires Subject: Random Vs Rational Date: 5 Oct 1999 04:08:21 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 28 Sender: daemon@net.bio.net Approved: A.Wallace@Queens-Belfast.AC.UK Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear listmembers: I would like to know your views on an interesting topic " Random subset selection for screening combinatorial libraries Vs Rational selection of compounds from the diversity space" Which, you think, has more advantages in case combinatorial libraries. random screening programs are still very important in order to discover drugs or leads that have unexpected and unusual structures for various targets. Also they lower time associated with intellectualization in rational selection. Saves money associated with the use of various softwares. On the other hand rational selection makes it possible to deal with the lower number of molecule in the library and may increases the probability of finding a lead compound. 1) Where your vote goes? 2) Also I am looking for any statistic you have on the success of Random Vs Rational screening. Thanking you in anticipation Amit amitkg@giasbmc.vsnl.net.in From owner-repertoires@net.bio.net Fri Oct 08 07:41:00 1999 Path: biosci!biosci!not-for-mail From: felici@mclink.it (Franco Felici) Newsgroups: bionet.molecules.repertoires Subject: Re: Random Vs Rational Date: 8 Oct 1999 01:41:09 -0700 Organization: MRC Human Genome Mapping Project Resource Centre Lines: 40 Sender: daemon@net.bio.net Approved: A.Wallace@Queens-Belfast.AC.UK Distribution: world Message-ID: <37FC5FDC.AB5B36BA@mclink.it> References: NNTP-Posting-Host: net.bio.net FYI, last year Anna Tramontano and me wrote a chapter for a book about this very topic, if anybody is interested: A. Tramontano and F. Felici "Exploring molecular recognition by combinatorial and rational approaches" in: "Protein engineering in industrial biotechnology", Ed. L. Alberghina; Harwood Academic Publishers, Amsterdam, The Netherlands, 275-289 - 1999 Regards, Franco Felici Amit Galande wrote: > > Dear listmembers: > > I would like to know your views on an interesting topic > > " Random subset selection for screening combinatorial libraries Vs > Rational selection of compounds from the diversity space" > > Which, you think, has more advantages in case combinatorial libraries. > > random screening programs are still very important in order to discover > drugs or leads that have unexpected and unusual structures for various > targets. Also they lower time associated with intellectualization in > rational selection. Saves money associated with the use of various > softwares. On the other hand rational selection makes it possible to deal > with the lower number of molecule in the library and may increases the > probability of finding a lead compound. > 1) Where your vote goes? > 2) Also I am looking for any statistic you have on the success of Random > Vs Rational screening. > Thanking you in anticipation > Amit > amitkg@giasbmc.vsnl.net.in --- From owner-repertoires@net.bio.net Tue Oct 12 11:04:00 1999 Path: biosci!biosci!not-for-mail From: Mike Briley Newsgroups: bionet.molecules.repertoires Subject: Symposium on Molecular Genetics in Mental Disorders Date: 12 Oct 1999 05:04:28 -0700 Organization: ImagiNET Lines: 12 Sender: daemon@net.bio.net Approved: A.Wallace@Queens-Belfast.AC.UK Distribution: world Message-ID: <7tv84c$9fa@net.bio.net> NNTP-Posting-Host: net.bio.net There is still time (just) to register for the Symposium on Molecular Genetics in Mental Disorders that will take place in Castres, France 1-3 December 1999. Full details (including registration forms) are available from http://www.entretiens-du-carla.com Mike Briley From owner-repertoires@net.bio.net Wed Oct 13 09:32:00 1999 Path: biosci!biosci!not-for-mail From: ASINEX Ltd Newsgroups: bionet.molecules.repertoires Subject: New Web site for Combichem Date: 13 Oct 1999 03:32:09 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Approved: A.Wallace@Queens-Belfast.AC.UK Distribution: world Message-ID: <01bf14e7$f9aa89c0$LocalHost@default> NNTP-Posting-Host: net.bio.net Dear Netters, HTTP://WWW.COMBICHEM.NET New site dedicated to chemistry and HTS. Meetings, chemicals, libraries, methods, corporate news - everything is there. See your postings at combichem.net! Oleg From owner-repertoires@net.bio.net Tue Oct 19 16:47:00 1999 Path: biosci!biosci!not-for-mail From: "Dr. Wendy A. Warr" Newsgroups: bionet.molecules.repertoires Subject: "Chemoinformatics" Date: 19 Oct 1999 10:47:15 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 34 Sender: daemon@net.bio.net Approved: A.Wallace@Queens-Belfast.AC.UK Distribution: world Message-ID: <3805EAF9.308A@warr.com> NNTP-Posting-Host: net.bio.net Colleagues, I had agreed to summarize for these lists (excuse multiple postings) all the comments I received about cheminformatics/chemoinformatics/chemiiformatics etc. Actually I had decided that the easiest thing would be to refer you to my slides on the ACS COMP site, where you could also see the slides of other speakers in this ACS New Orleans symposium. Unfortunately there have been some problems in putting the slides onto the Web site. There is also a possibility that the symposium proceedings will appear in a book and it has been suggested that it would be inadvisable to put my results up on a Web site or a list for copyright reasons. However, I DO intend to put my talk into my usual (commercial) meeting report so I have to resolve the copyright issue soon. I just wanted to thank everyone who gave me their suggestions, and let you know that I have not forgotten about the requested summary. Wendy -- Dr Wendy A Warr Wendy Warr & Associates, 6 Berwick Court Holmes Chapel, Cheshire CW4 7HZ, England Tel/fax +44 (0)1477 533837 wendy@warr.com http://www.warr.com From owner-repertoires@net.bio.net Tue Oct 19 16:51:00 1999 Path: biosci!biosci!not-for-mail From: Marta Fanciullo Newsgroups: bionet.molecules.repertoires Subject: combinatorial drugs in clinical trials Date: 19 Oct 1999 10:51:08 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Approved: A.Wallace@Queens-Belfast.AC.UK Distribution: world Message-ID: <199910150706.JAA20722@giove.caspur.it> NNTP-Posting-Host: net.bio.net Dear listmembers, I am looking for a list of drugs, coming from combinatorial projects that are in clinical trials. Does anyone know some references about this topic? Furthermore: Does anyone know a statistic about the numbers of drug candidates developed using combinatorial technologies vs. traditional? Thanking you in anticipation Marta Fanciullo fanciullo@tecnofarmaci.caspur.it From owner-repertoires@net.bio.net Tue Oct 26 13:48:00 1999 Path: biosci!biosci!not-for-mail From: Craig Belcher Newsgroups: bionet.molecules.repertoires Subject: know of an scFV to a short polypeptide epitope? Date: 26 Oct 1999 07:48:47 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Approved: A.Wallace@Queens-Belfast.AC.UK Distribution: world Message-ID: <8F262BE01D57D3119708005004B07BA00110C4@SBS> NNTP-Posting-Host: net.bio.net I am trying to find a scFv which binds to a short polypeptide epitope with a dissociation constant of 10 nM or better. Preferrably, the epitope would have been characterised as an N-terminal tag. Does anyone have any suggestions or know of any papers? From owner-repertoires@net.bio.net Wed Oct 27 09:03:00 1999 Path: biosci!biosci!not-for-mail From: tvink@xs4all.nl (Tom Vink) Newsgroups: bionet.molecules.repertoires Subject: Re: know of an scFV to a short polypeptide epitope? Date: 27 Oct 1999 03:03:40 -0700 Organization: XS4ALL Internet BV Lines: 15 Sender: daemon@net.bio.net Approved: A.Wallace@Queens-Belfast.AC.UK Distribution: world Message-ID: <3816b665.4978939@news.xs4all.nl> References: <8F262BE01D57D3119708005004B07BA00110C4@SBS> NNTP-Posting-Host: net.bio.net On 26 Oct 1999 07:48:47 -0700, Craig Belcher wrote: >I am trying to find a scFv which binds to a short polypeptide epitope with a >dissociation constant of 10 nM or better. Preferrably, the epitope would >have been characterised as an N-terminal tag. Does anyone have any >suggestions or know of any papers? > > > No, I only know about one which binds a C-terminal His tag, can you let me know if you find one ?