From owner-molreps@hgmp.mrc.ac.uk Wed Jul 5 09:20:54 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 52AC617A8B; Wed, 5 Jul 2000 09:20:54 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 3D1B617AC3; Wed, 5 Jul 2000 09:20:53 +0100 (BST) To: molreps@net.bio.net Newsgroups: bionet.molecules.repertoires From: kaczorek@polnet.cc ("jk") Subject: IM LOOKING FOR MOLECULAR DYNAMICS TYPHOON 8600 - USERS WORLDWIDE Date: 30 Jun 2000 16:59:57 +0100 Organization: http://news.icm.edu.pl/ Message-ID: <8jig52$3b0$1@sunsite.icm.edu.pl> Sender: owner-molreps@hgmp.mrc.ac.uk Precedence: bulk I am interested to buy TYPHOON 8600 and I am looking for users opinion. If you use this equipment please contact to my e-mail. jk --- From owner-molreps@hgmp.mrc.ac.uk Thu Jul 6 18:20:18 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 8925117AB0; Thu, 6 Jul 2000 18:20:17 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 8917817AE6; Thu, 6 Jul 2000 18:20:16 +0100 (BST) To: molreps@net.bio.net Newsgroups: bionet.molecules.repertoires From: Christine Dempster Subject: PHAGE DISPLAY CONFERENCE Date: 6 Jul 2000 18:04:20 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-molreps@hgmp.mrc.ac.uk Precedence: bulk If you have previously received this e-mail announcement please accept our apologies for the duplicate notification. Conference Announcement: PHAGE DISPLAY CONFERENCE September 21 - 24, 2000 Vancouver, B.C. Canada We are pleased to inform you that the Phage Display Conference web page is: http://www.sfu.ca/phagedisplay2000/ You can access the conference agenda and registration form from this web site. Please make special note of the following: Accommodation and Airline Alert If you are planning on attending the conference, we urge you to make your hotel and travel arrangements immediately. There are over five large conferences in town during the week of September 16-24 and it is anticipated that Vancouver hotel rooms and flights into the city will fill up quickly. To avoid disappointment, please do not wait to make your reservations if you plan on attending the Phage Display conference. --- From owner-molreps@hgmp.mrc.ac.uk Mon Jul 31 11:15:37 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id E352017AAB; Mon, 31 Jul 2000 11:15:36 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 820E417AC8; Mon, 31 Jul 2000 11:15:35 +0100 (BST) To: molreps@net.bio.net Newsgroups: bionet.molecules.repertoires From: zhaojun@public.wh.hb.cn (zhaojun) Subject: Help Date: 23 Jul 2000 11:39:59 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <009301bff492$847313c0$0fae6eca@default> Sender: owner-molreps@hgmp.mrc.ac.uk Precedence: bulk Dear Sir, We try to clone a the double stranded Oligos ( with Hind III and XbaI at each end of the Oligo) into pcDNA3 vector, but I don't know whether the Oligos should be phosphated? If yes, would you please provide a detail protocol for phosphorylation using T4 polynucleotide kinase? In addition, what kind of ATP should be used in the reaction? Thanks Zhaojun, MD From owner-molreps@hgmp.mrc.ac.uk Mon Jul 31 11:16:27 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 52E1917ACE; Mon, 31 Jul 2000 11:16:25 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 11DF717ACE; Mon, 31 Jul 2000 11:16:23 +0100 (BST) To: molreps@net.bio.net Newsgroups: bionet.molecules.repertoires From: zhaojun@public.wh.hb.cn (zhaojun) Subject: GFP Date: 24 Jul 2000 04:56:48 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <006d01bff521$752ef200$8bac6eca@default> Sender: owner-molreps@hgmp.mrc.ac.uk Precedence: bulk Dear Sir, I am a doctor graduate student of Tongji Medical University in Wuhan, China. My research field is gene therapy of human bladder cancer. In these days, I am studying on the construction of eukaryotic expression vector for bladder cancer tissue in which the plasmid containing the green fluorescent protein (GFP)is needed.Would you please supply me this kind of plasmid or some information about it? Thank you very much. Sincerely, Tong Qiangsong, Ph.D. Department of Urology Union Hospital Tongji Medical University Wuhan,430022 P.R. China