From owner-molreps@hgmp.mrc.ac.uk Tue Oct 16 12:06:42 2001 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id D996517ADD for ; Tue, 16 Oct 2001 12:06:41 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id E9CA117ADE for ; Tue, 16 Oct 2001 12:06:38 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 6CF7017ADD; Tue, 16 Oct 2001 12:06:35 +0100 (BST) To: molreps@net.bio.net Newsgroups: bionet.molecules.repertoires From: mdp96tab@shef.ac.uk (Tom Barr) Subject: Variable region gene sequence Date: 15 Oct 2001 15:23:22 +0100 Organization: http://groups.google.com/ Message-ID: <124f7f4b.0110150623.7e84c1e1@posting.google.com> Sender: owner-molreps@hgmp.mrc.ac.uk Precedence: bulk Dear All, I write to enquire about the availability of sequence information for the hamster Ig variable regions. Specifically, I'm hoping to try a M13 phage based display of recombinant antibodies from a Syrian hamster cell line. I'm having difficulty finding sequence data and wonder if any of you have any experience of hamster-phage recombinant antibodies, particularly with regards to successful primers... Any advice would be greatly appreciated. Cheers, -- Tom Barr Division of Genomic Medicine (F-Floor) University of Sheffield Medical School Beech Hill Road Sheffield S10 2RX Tel.: +44 (0)114 271 2983 Fax: +44 (0)114 273 9926 ---