From BIOSCI-REQUEST Sun Dec 1 20:54:33 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id UAA12515; Sun, 1 Dec 1996 20:54:33 -0800 Received: from muwayb.ucs.unimelb.EDU.AU (muwayb.ucs.unimelb.EDU.AU [128.250.20.7]) by net.bio.net (8.6.12/8.6.6) with ESMTP id UAA12465; Sun, 1 Dec 1996 20:54:14 -0800 From: humut@ariel.ucs.unimelb.edu.au Received: from mur098 (stvpc05.medstv.unimelb.EDU.AU) by muwayb.ucs.unimelb.edu.au (PMDF V5.0-7 #17781) id <01ICJGDTMU7K000H85@muwayb.ucs.unimelb.edu.au> for mutation@net.bio.net; Mon, 02 Dec 1996 15:54:28 +1000 Date: Mon, 02 Dec 1996 14:51:22 +0000 Subject: Nomenclature proposal on web X-Sender: humut@ariel.ucs.unimelb.edu.au To: mutation@net.bio.net Message-id: <01ICJGDUD01U000H85@muwayb.ucs.unimelb.edu.au> MIME-version: 1.0 X-Mailer: Windows Eudora Version 1.4.4 Content-type: text/plain; charset="us-ascii" Content-transfer-encoding: 7BIT The Recommendations for a Nomenclature System for Human Gene Mutations prepared by Stylianos Antonarakis has now been posted on the Mutation Database Website. To access this directly point your server to: http:ariel.ucs.unimelb.edu.au:80/~cotton/antonara.htm Or, for the whole website: http:ariel.ucs.unimelb.edu.au:80/~cotton/mut_database.htm Rania Horaitis Mutation Research Centre 7th floor Daly Wing, St. Vincent's Hospital 41 Victoria Pde Fitzroy, VIC 3065 Australia Fax: 61-3-9288-2989 email:horaitis@ariel.ucs.unimelb.edu.au From BIOSCI-REQUEST Fri Dec 6 02:00:33 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id CAA05497; Fri, 6 Dec 1996 02:00:33 -0800 Received: (from biohelp@localhost) by net.bio.net (8.6.12/8.6.6) id CAA05472; Fri, 6 Dec 1996 02:00:15 -0800 Date: Fri, 6 Dec 1996 02:00:15 -0800 From: BIOSCI Administrator Message-Id: <199612061000.CAA05472@net.bio.net> To: mutation@net.bio.net Subject: BIOSCI/bionet miniFAQ & Fundraiser (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From BIOSCI-REQUEST Wed Dec 11 16:04:26 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id QAA24761; Wed, 11 Dec 1996 16:04:26 -0800 Received: from audrey.tli.org (AUDREY.TLI.ORG [192.92.10.18]) by net.bio.net (8.6.12/8.6.6) with SMTP id QAA24750; Wed, 11 Dec 1996 16:04:09 -0800 Received: from ([204.134.37.206]) by audrey.tli.org (4.1/SMI-4.1) id AA15795; Wed, 11 Dec 96 17:12:57 MST Message-Id: <32AF59BC.1FD5@lucy.tli.org> Date: Wed, 11 Dec 1996 17:02:52 -0800 From: Jim Gale X-Mailer: Mozilla 3.01Gold (Win16; I) Mime-Version: 1.0 To: mutation@net.bio.net Subject: SSCP Help Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Greetings mutation folks I am setting up SSCP in my lab and I need some advice: 1) What gel rigs do you like ? (quality, but inexpensive, temp-controlled) 2) Any experience with FMC's MDE gel matrix ??? 3) I am looking for mutations in a repeated gene family, so that my initial PCR represents a population of genes and must be subcloned. Now, would it be possible to run a restriction digest of these mini-preped clones that cuts out the small gene (and the 3 kb plasmid) directly on the SSCP gel (after some clean-up or desalting), or do I need to do another PCR for each clone ?? All help greatly appreciated....It would be nice to get it right the first time Thanks Jim Gale jim@lucy.tli.org From BIOSCI-REQUEST Thu Dec 12 13:47:37 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id NAA04937; Thu, 12 Dec 1996 13:47:37 -0800 Received: from wanda.vf.pond.com (wanda.vf.pond.com [198.69.82.2]) by net.bio.net (8.6.12/8.6.6) with ESMTP id NAA04913; Thu, 12 Dec 1996 13:47:21 -0800 From: nrt@pond.com Received: by wanda.vf.pond.com (8.6.12/gw.1.0) id QAA15757; Thu, 12 Dec 1996 16:48:21 -0500 Date: Thu, 12 Dec 1996 16:48:20 -0500 (EST) Subject: Re: SSCP Help To: Jim Gale cc: mutation@net.bio.net In-Reply-To: <32AF59BC.1FD5@lucy.tli.org> Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII On Wed, 11 Dec 1996, Jim Gale wrote: > Greetings mutation folks > > I am setting up SSCP in my lab and I need some advice: > 1) What gel rigs do you like ? (quality, but inexpensive, > temp-controlled) > 2) Any experience with FMC's MDE gel matrix ??? > 3) I am looking for mutations in a repeated gene family, so that my > initial PCR represents a population of genes and must be subcloned. Now, > would it be possible to run a restriction digest of these mini-preped > clones that cuts out the small gene (and the 3 kb plasmid) directly on > the SSCP gel (after some clean-up or desalting), or do I need to do > another PCR for each clone ?? > > All help greatly appreciated....It would be nice to get it right the > first time > > Thanks > Jim Gale > jim@lucy.tli.org > You might look into the LongRanger gels marketed by JT Baker based upon hydrolink technology. I beleive they have a specific gel for SSCP. It gave us good results. Nathan Tzodikov, Ph. D. Magainin Pharmaceuticals, Inc. From BIOSCI-REQUEST Sun Dec 15 06:02:09 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id GAA04259; Sun, 15 Dec 1996 06:02:09 -0800 Received: from brainy1.ie-eg.com (brainy1.ie-eg.com [194.79.96.40]) by net.bio.net (8.6.12/8.6.6) with SMTP id GAA04173; Sun, 15 Dec 1996 06:01:51 -0800 From: lec@brainy1.ie-eg.com Received: from ppp25.ie-eg.com by brainy1.ie-eg.com; (5.65v3.2/1.1.8.2/24Aug96-0523PM) id AA27071; Sun, 15 Dec 1996 16:02:15 +0200 Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Mon, 16 Dec 1996 04:14:52 +0200 To: mutation@net.bio.net subcribe mutation From BIOSCI-REQUEST Sun Dec 15 06:51:36 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id GAA08967; Sun, 15 Dec 1996 06:51:36 -0800 Received: from brainy1.ie-eg.com (brainy1.ie-eg.com [194.79.96.40]) by net.bio.net (8.6.12/8.6.6) with SMTP id GAA08959; Sun, 15 Dec 1996 06:51:17 -0800 From: lec@brainy1.ie-eg.com Received: from ppp24.ie-eg.com by brainy1.ie-eg.com; (5.65v3.2/1.1.8.2/24Aug96-0523PM) id AA09966; Sun, 15 Dec 1996 16:51:39 +0200 Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Mon, 16 Dec 1996 05:04:17 +0200 To: mutation@net.bio.net subscribe mutation From BIOSCI-REQUEST Wed Dec 18 16:35:37 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id QAA07455; Wed, 18 Dec 1996 16:35:37 -0800 Received: from WALTHM.CRIC.COM (cric.com [192.138.207.10]) by net.bio.net (8.6.12/8.6.6) with SMTP id QAA07442; Wed, 18 Dec 1996 16:35:15 -0800 Message-Id: <199612190035.QAA07442@net.bio.net> Received: from [192.138.207.84] by 192.138.207.84 with SMTP; Wed, 18 Dec 1996 19:34:25 -0500 (EST) Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Wed, 18 Dec 1996 20:37:39 -0500 To: mutation@net.bio.net From: call@cric.com (Kathy Call) Subject: Ph.D. Mutation Detection Position Open Cc: lomedico, keith, weiffenbach, osullivan, call The Human Genetics Department at Genome Therapeutics Corporation (Waltham, MA) has an opening for a Ph.D. level scientist in Mutation Detection and Disease Gene Analysis. Research involves mutation analysis in genes involved in cancer, osteoporosis, asthma and other diseases. The position also involves implementation and development of new methodologies, high-throughput assays, and mutation detection in a variety of samples. Candidate should have proven track record in the field of mutation detection and analysis and the ability to lead and train scientists. Background in human genetics, bioinformatics (particularly databases) and/or high-throughput assays a plus. Additional Ph.D. level positions are open currently in Disease Gene Discovery in the Human Genetics Department. Genome Therapeutics Corporation (GTC)is a leader in the exciting field of genome research, and a major contributor in the Human Genome Project. Our focus is to identify and characterize genes involved in common diseases and to translate our discoveries into therapeutic breakthroughs. GTC is a fast growing and financially successful biotechnology company--in the past year we have initiated a $22 million alliance with Astra AB to develop genome-based drugs and vaccines, a $43 million alliance with Schering-Plough to discover novel anti-infective agents effective against drug-resistant organisms, and been awarded over $10 million by NIH to develop state-of-the-art DNA sequencing technology. GTC offers an excellent compensation and benefits package. Please send or fax your resume to: Human Resources Genome Therapeutics Corporation 100 Beaver Street Waltham, MA 02154 fax 617-893-9535 Reference that you are applying for the Ph.D. level Mutation Detection position in Human Genetics (Job code: KMC). An Equal Opportunity Employer M/F/H/V. Katherine Call, Ph.D. Research Manager Human Genetics Genome Therapeutics Corp. 1365 Main Street Waltham, MA 02154 Call@cric.com (e-mail)