From BIOSCI-REQUEST Tue Jan 6 02:00:10 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id CAA23166; Tue, 6 Jan 1998 02:00:10 -0800 Received: (from biohelp@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id CAA23148; Tue, 6 Jan 1998 02:00:07 -0800 Date: Tue, 6 Jan 1998 02:00:07 -0800 From: BIOSCI Administrator Message-Id: <199801061000.CAA23148@net.bio.net> To: mutation@net.bio.net Subject: BIOSCI/bionet miniFAQ & Fundraiser (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From BIOSCI-REQUEST Thu Jan 8 01:36:42 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id BAA06564; Thu, 8 Jan 1998 01:36:42 -0800 Received: from cony.gsf.de (cony.gsf.de [146.107.1.2]) by net.bio.net (8.6.12-r-beta/8.6.6) with ESMTP id BAA06559; Thu, 8 Jan 1998 01:36:40 -0800 Received: from PC1635 by cony.gsf.de (8.6.4/WeissBlau-1.10) id KAA26785; Thu, 8 Jan 1998 10:38:28 +0100 Message-Id: <3.0.2.16.19980108103502.4017dcfc@pop.gsf.de> X-Sender: wjst@pop.gsf.de X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.2 (16) Date: Thu, 08 Jan 1998 10:35:02 -0100 To: mutation@net.bio.net From: Matthias Wjst Subject: Asthma Gene Database at cooke.gsf.de Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Dear colleagues, we are happy to announce a new internet database for asthma and allergy linkages and mutations. The address is http://cooke.gsf.de R.A. Cooke has written the first major paper on asthma genetics and the GSF is a national German research center located in the north of Munich. As you can enter and change data from every part of the world we have implemented a password restriction. After registration you will be immediately emailed your password. Let us know if you have any problems to login. Be sure that your browser is JAVA enabled as we generate figures on the fly from the database. The data entry has just started. If you do not find you own results there, please follow the step by step procedures described in the help texts to enter your own results. Please feel free to send your comments to me or write them on the online blackboard. Yours sincerely / Mit :-) Gruessen, Matthias Wjst Institut fuer Epidemiologie, GSF Forschungszentrum fuer Umwelt und Gesundheit, Ingolstaedter Landstrasse 1, D-85764 Neuherberg/ Munich, Germany, phone +49-89-3187-4565, fax +49-89-3187-3380 email wjst@gsf.de From BIOSCI-REQUEST Fri Jan 9 10:37:33 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id KAA19393; Fri, 9 Jan 1998 10:37:33 -0800 Received: from snipe.biotech.ufl.org (snipe.biotech.ufl.org [128.227.25.4]) by net.bio.net (8.6.12-r-beta/8.6.6) with ESMTP id KAA19383; Fri, 9 Jan 1998 10:37:31 -0800 Received: from biotech.ufl.org by snipe.biotech.ufl.org (8.7/4.09) id NAA24036; Fri, 9 Jan 1998 13:36:44 -0500 (EST) Message-ID: <34B66DDB.1246EB59@biotech.ufl.org> Date: Fri, 09 Jan 1998 13:35:08 -0500 From: Martin Munzer Reply-To: mmunzer@biotech.ufl.org Organization: CyGene, Inc. X-Mailer: Mozilla 4.02 [en]C-DIAL (WinNT; U) MIME-Version: 1.0 To: mutation@net.bio.net Subject: New DNA technology Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit New DNA technology The following text is a press release sent out Wednesday through PRNewswire. Contrary to the hype and hysteria over human cloning, these technologies will prove of great benefit to mankind and be available soon. If this does not interest you, please forward it to someone who might be interested. Thanks, Martin Munzer PRESS RELEASE Florida Biotech Companies Fill DNA Technology Gap with New Diagnostic Technologies Alachua, Florida, Jan. 7, /PRNewswire/ -- AGENDA, Inc. and CyGene, Inc., two Florida based biotech companies, have signed a co-licensing agreement to market their next-generation DNA diagnostic technologies. Under the terms of the agreement, AGENDA will market CyGene's patent pending TPA (TM) technology exclusively, and in conjunction with its own RFTA (TM) technology. 1997 was an exceptional year for DNA discoveries. Important genetic sequences, some responsible for diseases such as Alzheimer's, Rheumatoid Arthritis, Cancers, Heart Disease, etc. were recognized. These were added to the huge arsenal of already recognized genetic sequences like HIV, Hepatitis C and numerous contagious or hereditary diseases. One critical tool missing, which would add to the value of these discoveries, is a technology that will quickly, reliably and economically recognize these specific genes when only a few copies are present. AGENDA's RFTA (TM) (patent pending) technology complements TPA (TM) in that both will detect any known genetic sequence while using different methods. These technologies make early reliable diagnosis of HIV, Hepatitis C, cancer, parasitic, viral, bacterial and hereditary diseases a reality. Both processes can be automated, to provide fast and unprecedented first and second opinions regarding the presence or absence of the targeted genes. The ability of these technologies to handle large quantities of DNA or RNA set them apart from other techniques, which are prone to false results. For the first time, very early disease recognition (before the onset of clinical symptoms) is made possible by these next-generation DNA diagnostic technologies. This will open the door to entirely new approaches to disease prevention, management and cure. Early stage diagnosis will make cure scenarios a reality, for many terminal diseases. First generation DNA technology can only reliably detect HIV, 6-7 months post infection. TPA/RFTA (TM) will be able to detect HIV accurately shortly after the time of infection. Similar to computer operating systems, RFTA (TM) and TPA (TM) represent a technology base from which applications can be developed for agricultural, veterinary, health sciences, and human diagnostics. Based on proven molecular biology principles, both technologies can be configured to complete automation and may be performed in tandem using a single sample. AGENDA is actively seeking licensees and companies involved in the design and production of biotech automation equipment. This is the first public announcement of the existence of TPA (TM) and RFTA (TM). Contact: Martin Munzer, Executive VP, 904-418-1616 Email mmunzer@dna-diagnostics.com http://www.dna-diagnostics.com/ and http://www.cygene.com From BIOSCI-REQUEST Tue Jan 13 08:19:19 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id IAA27291; Tue, 13 Jan 1998 08:19:19 -0800 Received: from postoffice.webcow.com (postoffice.webcow.com [206.251.6.250]) by net.bio.net (8.6.12-r-beta/8.6.6) with ESMTP id IAA27286; Tue, 13 Jan 1998 08:19:16 -0800 Received: from cygene-2.biotech.ufl.org ([128.227.25.117]) by postoffice.webcow.com (Post.Office MTA v3.1 release PO203a ID# 0-41990U5000L500S0) with SMTP id AAA10272 for ; Tue, 13 Jan 1998 08:18:25 -0800 Received: by cygene-2.biotech.ufl.org with Microsoft Mail id <01BD2014.A23810E0@cygene-2.biotech.ufl.org>; Tue, 13 Jan 1998 11:16:02 -0500 Message-ID: <01BD2014.A23810E0@cygene-2.biotech.ufl.org> From: mmunzer@dna-diagnostics.com (dna-diagnostics.com) To: "'mutation@net.bio.net'" Subject: Re: New DNA Technologies Date: Tue, 13 Jan 1998 11:15:49 -0500 MIME-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Content-Transfer-Encoding: quoted-printable Thank you all for your overwhelmingly positive response to our = announcement of new DNA Diagnostic technologies. Due to the volume of = inquiries, we have compiled a series of FAQ's, to respond to the = majority of your questions. We have posted them on our CyGene web site = along with several letters of opinion from professionals who have = reviewed the TPA and RFTA patent documents. RFTA opinion letters will be = posted on our AGENDA site shortly. We will respond to all other inquiries as soon as possible. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Martin Munzer=20 AGENDA, Inc. http://www.dna-diagnostics.com a.k.a. Advanced Genetic Diagnostic Associates=20 Confirming "Heirlines" Through State of the Art DNA Analysis ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From BIOSCI-REQUEST Tue Jan 13 21:50:19 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id VAA11683; Tue, 13 Jan 1998 21:50:19 -0800 Received: from mailgate32 (mailgate32-hme0.a001.sprintmail.com [205.137.196.58]) by net.bio.net (8.6.12-r-beta/8.6.6) with ESMTP id VAA11680 From: 1hotstock@sprintmail.com Received: by mailgate32 (SMI-8.6/SMI-SVR4) id LAA19197; Tue, 13 Jan 1998 11:16:13 -0800 Received: from sdn-ts-003mnminnp15.dialsprint.net(206.133.97.66) by mailfep2-hme1 via smap (KC5.24) id Q_10.1.1.6/Q_22203_129_34bbbd75; Tue Jan 13 11:16:05 1998 Date: Tue, 13 Jan 98 13:11:34 EST To: InternetUser@OnThe.Net Subject: HOT STOCK - Analyst Say JTSR Possibly Hits $5.00 by Mid Feb 98!! Message-ID: <> Start 1998 out with a Winner!!! Visit http://www.jtsr-stock.com for complete information on one of the hottest stocks of the new year! J.T.'S RESTAURANTS, INC. is currently undervalued and on the move! Don't miss this opportunity. Visit the web site at http://www.jtsr-stock.com to find out why. J.T.'S RESTAURANTS, INC. (NASDAQ OTC BB Symbol: JTSR) is one of the fastest growing restaurant chains in the country! Several top stock market analysts predicted last week that the stock will potentially reach $5.00 by the end of February 1998 and over $10.00 by year end! Analysts PROVEN RIGHT!! -JTSR DOUBLES Before Christmas 1997! If you had invested just $100 in McDonalds' in 1955, that stock would be millionaire today. A recent acquisition announcement that may add up to 10 new restaurant locations in 1998 has already resulted in the price moving from $1.35 last week to well over $2.25 in just 5 days! Catch the rising star of JTSR at http://www.jtsr-stock.com (NOTE: The information contained in this message does not constitute an offering or inducement to purchase securities. It is for information purposes only.) From BIOSCI-REQUEST Sat Jan 17 01:17:38 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id BAA26977; Sat, 17 Jan 1998 01:17:38 -0800 Received: from rs6a.wln.com (rs6a.wln.com [192.156.252.2]) by net.bio.net (8.6.12-r-beta/8.6.6) with ESMTP id BAA26974 From: 39387840@hotmail.com Received: from rs6a.wln.com (hdn93-136.hil.compuserve.com [206.175.100.136]) by rs6a.wln.com (8.6.11/4.06) via SMTP id BAA58860; Sat, 17 Jan 1998 01:16:07 -0800 env-from (39387840@hotmail.com) Date: Sat, 17 Jan 98 02:30:38 EST To: InternetUser@OnThe.Net Subject: Pay Off Those Holiday Bills!!! 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Send for the four reports IMMEDIATELY, so you will have them when the orders start coming in because: When you receive a $5 order, you MUST send out the requested product/ report to comply with the U.S. Postal & Lottery Laws, Title 18, Sections 1302 and 1341 or Title 18, Section 3005 in the U.S. Code, also Code of Federal Regs. vol. 16, Sections 255 and 436, which state that "a product or service must be exchanged for money received." * ALWAYS PROVIDE SAME-DAY SERVICE ON THE ORDERS YOU RECEIVE. * Be patient and persistent with this program. If you follow the instructions exactly the results WILL undoubtedly be SUCCESS! * ABOVE ALL, HAVE FAITH IN YOURSELF THAT YOU CAN SUCCEED! *******YOUR SUCCESS GUIDELINE******* The check point that guarantees your success is simply this: You MUST receive 10 to 20 orders for REPORT #1! THIS IS A MUST! If you don't within two weeks, advertise more and send out more programs until you do. Then, a couple of weeks later you should receive at least 100 orders for REPORT #2. If you don't, advertise more and send out more programs until you do. Once you have received 100, or more orders for REPORT #2, YOU CAN RELAX, because you will be on your way to the BANK! -OR- You can DOUBLE your efforts! REMEMBER: Every time your name is moved down on the list you are in front of a DIFFERENT report, so you can KEEP TRACK of your PROGRESS by what report people are ordering from you. IT'S THAT EASY!!! NOTE: IF YOU NEED HELP with starting a business, registering a business name, how income tax is handled, etc., contact your local office of the Small Business Administration (a Federal agency) for free help and answers to questions. Also, the Internal Revenue Service offers free help via telephone and free seminars about business taxes. ******* T E S T I M O N I A L S ******* This program does work, but you must follow it EXACTLY! Especially the rule of not trying to place your name in a different position, it won't work, you'll lose a lot of money. I'm living proof that it works. It really is a great opportunity to make relatively easy money, with little cost to you. If you do choose to participate, follow the program exactly, and you'll be on your way to financial security. If you are a fellow Christian and are in financial trouble like I was, consider this a sign. I DID! Good Luck & God Bless You, Sincerely, Chris Johnson P.S. Do you have any idea what 11,700 $5 bills ($58,500) looks like piled up on the kitchen table?...IT'S AWESOME! My name is Frank. My wife Doris and I live in Bel-Air, MD. I am a cost accountant with a major U.S. Corporation and I make pretty good money. When I received the program I grumbled to Doris about receiving "junk mail"! I made fun of the whole thing, spouting my knowledge of the population and percentages involved. I "knew" it wouldn't work. Doris totally ignored my supposed intelligence and jumped in with both feet. I made merciless fun of her, and was ready to lay the old "I told you so" on her when the thing didn't work... well, the laugh was on me! Within two weeks she had received over 50 responses. Within 45 days she had received over $147,200 in $5 bills! I was stunned. I was sure that I had it all figured and that it wouldn't work...I AM a believer now. I have joined Doris in her "little" hobby. I did have seven more years until retirement, but I think of the "rat race" and it's not for me...We owe it all to MLM. Frank T., Bel-Air, MD I just want to pass along my best wishes and encouragement to you. Any doubts you have will vanish when your first orders come in. I even checked with the U.S. Post Office to verify that the plan was legal. It definitely is! IT WORKS!!! Paul Johnson, Raleigh, NC This is the only realistic money-making offer I've ever received. I participated because this plan truly makes sense. I was surprised when the $5.00 bills started filling my mail box. By the time it tapered off I had received over 8,000 orders with over $40,000 in cash. Dozens of people have sent warm personal notes too, sharing the news of their good fortunes! It's been WONDERFUL. Carl Winslow Tulsa, OK The main reason for this letter is to convince you that this system is honest, lawful, extremely profitable, and is a way to get a large amount of money in a short time. I was approached several times before I hecked this out. I joined just to see what one could expect in return for the minimal effort and money required. Initially I let no one in the organization know that I was an attorney and, to my astonishment, I received $36,470.00 in the first 14 weeks, with money still coming in. Sincerely yours, Phillip A. Brown This plan works like GANG-BUSTERS!! So far I have had 9,735 total orders OVER $48,000!!! I hope I have sparked your own excitement, if you follow the program exactly, you could have the same success I have, if not better. Your success is right around the corner, but you must do a little work. Good Luck! G. Bank Not being the gambling type, it took me several weeks to make up my mind to participate in this plan. But conservative that I am I decided that the initial investment was so little that there was just no way that I wouldn't get enough orders to at least get my money back. Boy I was surprised when I found my medium-size post office box crammed with orders. After that it got so over-loaded that I had to start picking up my mail at the window. I'll make more money this year than any 10 years of my life before. The nice thing about this deal is that it doesn't matter where in the U.S. the people live. There simply isn't a better investment with a faster return. Mary Rockland, Lansing, MI I had received this program before. I deleted it, but later I wondered if I shouldn't have given it a try. Of course, I had no idea who to contact to get another copy, so I had to wait until I was e-mailed another program...11 months passed then it came...I didn't delete this one!...I made $41,000 on the first try!! D. Wilburn, Muncie, IN This is my third time to participate in this plan. We have quit our jobs, and quite soon we will buy a home on the beach and live off the interest on our money. The only way on earth that this plan will work for you is if you do it. For your sake, and for your family's sake don't pass up this golden opportunity. Remember, when you order your four reports, SEND CASH. Checks have to clear the bank and create too many delays. Good luck and happy spending! Charles Fairchild, Spokane, WA Typically when I look at a money-making deal I want to know if the company is strong, will it be here when it's time for my big pay off. In this crazy thing there is no company intervention for management to blow it. Just people like me ordering directly from the source! Interesting...I had a couple of projects I'd been trying to fund to no avail so I thought; Why not give it a try? Well 2 1/2 weeks later the orders started coming in. One project is funded and I'm sure the other will be soon! Marilynn St. Claire, Logan, UT ==================================================== We could be printing YOUR testimonial next!!! ORDER YOUR REPORTS TODAY AND GET STARTED DOWN THE ROAD TO YOUR FINANCIAL FREEDOM!!! From BIOSCI-REQUEST Mon Jan 19 17:12:38 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id RAA19251; Mon, 19 Jan 1998 17:12:38 -0800 Received: from mucc.mahidol.ac.th (sispn@mucc.mahidol.ac.th [202.28.166.40]) by net.bio.net (8.6.12-r-beta/8.6.6) with ESMTP id RAA19247; Mon, 19 Jan 1998 17:12:34 -0800 Received: from localhost (sispn@localhost) by mucc.mahidol.ac.th (8.8.8/8.8.2) with SMTP id IAA00886 for ; Tue, 20 Jan 1998 08:15:13 +0700 (GMT+0700) Date: Tue, 20 Jan 1998 08:15:13 +0700 (GMT+0700) From: Sa-nga Pattanakitsakul To: mutation@net.bio.net Subject: PCR of terminal hairpin structure of virus (fwd) Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII ---------- Forwarded message ---------- Date: Mon, 19 Jan 1998 15:44:34 +0700 (GMT+0700) From: Sa-nga Pattanakitsakul To: nobody@net.bio.net Subject: PCR of terminal hairpin structure of virus Dear Colleagues, I would appreciate receiving some comments or suggestions on how to do PCR for the region of terminal hairpin structure of virus. This virus contain single-stranded DNA and has palindromic hairpin structure at both terminal end which is unique structure of virus in Family of Parvovirus. Could anyone has experienced to do PCR at that regions. Please tell the protocal or procedure to approach this problem. Yours sincerely, Sa-nga Pattanakitsakul,D.Med.Sc Med.Mol.Biol.Unit 5th Fl.Microbiol.Bldg. Faculty of Medicine Siriraj Hospital Mahidol Univ. Prannok RD. Bangkok 10700 THAILAND TEL:66-2-4198256-7 FAX:66-2-4184793 From BIOSCI-REQUEST Tue Jan 20 03:28:44 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id DAA25079; Tue, 20 Jan 1998 03:28:44 -0800 Received: from hgmp.mrc.ac.uk (mercury.hgmp.mrc.ac.uk [193.62.192.80]) by net.bio.net (8.6.12-r-beta/8.6.6) with ESMTP id DAA25075; Tue, 20 Jan 1998 03:28:42 -0800 Received: from (root@localhost) by hgmp.mrc.ac.uk (SMI-8.6/HGMP-MAILER) id LAA17310 for mutation@net.bio.net.FILTERED; Tue, 20 Jan 1998 11:27:49 GMT Received: from tin.hgmp.mrc.ac.uk (tin [193.62.192.50]) by hgmp.mrc.ac.uk (SMI-8.6/HGMP-MAILER) id LAA17300 for <>; Tue, 20 Jan 1998 11:27:47 GMT From: tweissen@hgmp.mrc.ac.uk (Mr. T. Weissensteiner) Date: Tue, 20 Jan 1998 11:27:46 GMT Message-Id: <21891.199801201127@tin.hgmp.mrc.ac.uk> To: mutation@net.bio.net, sispn@mahidol.ac.th Subject: Re: PCR of terminal hairpin structure of virus (fwd) Dear Dr. Pattanakitsakul I don't know exactly what problem these hairpins are causing you, but adding a dsDNA destabilizing additive (like DMSO, glycerol, or betaine) to your PCR buffer might help. Here are two references, specifically concerning DNA hairpins: Q Chou: Minimizing deletion artifacts during Taq polymerase PCR by E. coli SSB. Nucleic Acids Research (1992) 20: 4371 W-H Chen, B Hohn: DMSO improves PCR amplification of DNA with complex secondary structure. Trends in Genetics (1992) 8: 227 Please contact me if you have any further questions! Sincerely Thomas Weissensteiner Autoimmunity Group Jenner Institute for Vaccine Research Compton / Newbury Berkshire RG20 7NN T : 0044 1635 577920 FAX : 0044 1625 577901 email : tweissen@hgmp.mrc.ac.uk ---{#$:> <:$#}-~~ From BIOSCI-REQUEST Tue Jan 20 21:48:47 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id VAA09146; Tue, 20 Jan 1998 21:48:47 -0800 Received: from mucc.mahidol.ac.th (sispn@mucc.mahidol.ac.th [202.28.166.40]) by net.bio.net (8.6.12-r-beta/8.6.6) with ESMTP id VAA09134; Tue, 20 Jan 1998 21:48:28 -0800 Received: from localhost (sispn@localhost) by mucc.mahidol.ac.th (8.8.8/8.8.2) with SMTP id MAA24978; Wed, 21 Jan 1998 12:50:27 +0700 (GMT+0700) Date: Wed, 21 Jan 1998 12:50:27 +0700 (GMT+0700) From: Sa-nga Pattanakitsakul To: "Mr. T. Weissensteiner" cc: mutation@net.bio.net Subject: Re: PCR of terminal hairpin structure of virus (fwd) In-Reply-To: <21891.199801201127@tin.hgmp.mrc.ac.uk> Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Dear Dr.Weissensteiner, Thank you your response. I have tried to use the addiitive that you mentiones but it is not work well. The problem that I raised to the net user is how to do PCR size 700 bp spanning from the long terminal palindromic hairpin end (about 150 bp). The results of PCR that show small size and appeared some missing sequence in the middle part of PCR products. Could you suggest me any experiments to solve this problem. Yours sincerely, Sa-nga Pattanakitsakul,D.Med.Sc On Tue, 20 Jan 1998, Mr. T. Weissensteiner wrote: > Dear Dr. Pattanakitsakul > > I don't know exactly what problem these hairpins are causing you, > but adding a dsDNA destabilizing additive (like DMSO, glycerol, > or betaine) to your PCR buffer might help. > Here are two references, specifically concerning DNA hairpins: > Q Chou: Minimizing deletion artifacts during Taq polymerase PCR > by E. coli SSB. > Nucleic Acids Research (1992) 20: 4371 > W-H Chen, B Hohn: DMSO improves PCR amplification of DNA with > complex secondary structure. > Trends in Genetics (1992) 8: 227 > > Please contact me if you have any further questions! > > Sincerely > > > Thomas Weissensteiner > Autoimmunity Group > Jenner Institute for Vaccine Research > Compton / Newbury > Berkshire RG20 7NN > > T : 0044 1635 577920 > FAX : 0044 1625 577901 > email : tweissen@hgmp.mrc.ac.uk > > ---{#$:> <:$#}-~~ > From BIOSCI-REQUEST Wed Jan 21 03:34:34 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id DAA10831; Wed, 21 Jan 1998 03:34:34 -0800 Received: from hgmp.mrc.ac.uk (mercury.hgmp.mrc.ac.uk [193.62.192.80]) by net.bio.net (8.6.12-r-beta/8.6.6) with ESMTP id DAA10822; Wed, 21 Jan 1998 03:34:26 -0800 Received: from (root@localhost) by hgmp.mrc.ac.uk (SMI-8.6/HGMP-MAILER) id LAA04242 for mutation@net.bio.net.FILTERED; Wed, 21 Jan 1998 11:33:27 GMT Received: from tin.hgmp.mrc.ac.uk (tin [193.62.192.50]) by hgmp.mrc.ac.uk (SMI-8.6/HGMP-MAILER) id LAA04230 for <>; Wed, 21 Jan 1998 11:33:25 GMT From: tweissen@hgmp.mrc.ac.uk (Mr. T. Weissensteiner) Date: Wed, 21 Jan 1998 11:33:25 GMT Message-Id: <17193.199801211133@tin.hgmp.mrc.ac.uk> To: sispn@mahidol.ac.th, tweissen@hgmp.mrc.ac.uk Subject: Re: PCR of terminal hairpin structure of virus (fwd) Cc: mutation@net.bio.net Dear Dr. Pattanakitsakul The only way I can see is to try a number of different additives a various concentrations. It may be also necessary to adjust the MgCl2 concentration separately for each of the additives. In short: a lot of titration experiments! If you tell me what you have already done, I may be able to suggest some further experiments. Sincerely Thomas Weissensteiner Autoimmunity Group Jenner Institute for Vaccine Research Compton / Newbury Berkshire RG20 7NN T : 0044 1635 577920 FAX : 0044 1625 577901 email : tweissen@hgmp.mrc.ac.uk ---{#$:> <:$#}-~~ > Thank you your response. I have tried to use the addiitive that you > mentiones but it is not work well. The problem that I raised to the net > user is how to do PCR size 700 bp spanning from the long terminal > palindromic hairpin end (about 150 bp). The results of PCR that show > small size and appeared some missing sequence in the middle part of PCR > products. Could you suggest me any experiments to solve this problem. > Yours sincerely, > Sa-nga Pattanakitsakul,D.Med.Sc >> I don't know exactly what problem these hairpins are causing you, >> but adding a dsDNA destabilizing additive (like DMSO, glycerol, >> or betaine) to your PCR buffer might help. >> Here are two references, specifically concerning DNA hairpins: >> Q Chou: Minimizing deletion artifacts during Taq polymerase PCR >> by E. coli SSB. >> Nucleic Acids Research (1992) 20: 4371 >> W-H Chen, B Hohn: DMSO improves PCR amplification of DNA with >> complex secondary structure. >> Trends in Genetics (1992) 8: 227 From BIOSCI-REQUEST Wed Jan 21 15:18:23 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id PAA18329; Wed, 21 Jan 1998 15:18:23 -0800 Received: from muwayb.ucs.unimelb.EDU.AU (muwayb.ucs.unimelb.EDU.AU [128.250.20.7]) by net.bio.net (8.6.12-r-beta/8.6.6) with ESMTP id PAA18326; Wed, 21 Jan 1998 15:18:19 -0800 Received: from stvpc10.medstv.unimelb.EDU.AU ([128.250.216.60]) by muwayb.ucs.unimelb.edu.au (PMDF V5.1-8 #24554) with SMTP id <01ISO96TENGM000KNO@muwayb.ucs.unimelb.edu.au> for mutation@net.bio.net; Thu, 22 Jan 1998 09:59:02 +1100 Date: Thu, 22 Jan 1998 09:59:02 +1100 Date-warning: Date header was inserted by muwayb.ucs.unimelb.edu.au From: Rania Horaitis Subject: messages X-Sender: horaitis@ariel.ucs.unimelb.edu.au To: mutation@net.bio.net Message-id: <01ISO96UKENE000KNO@muwayb.ucs.unimelb.edu.au> MIME-version: 1.0 X-Mailer: Windows Eudora Light Version 1.5.2 Content-type: text/plain; charset="us-ascii" Dear Subscribers, Please keep in mind messages mailed to "mutation" will be received by ALL the subscribers. If you wish to answer someones question, please use their personal email address, do not press reply unless you think the entire community will benefit from the message. Thank you. Rania Horaitis Co-Ordinator HUGO Mutation Database Initiative horaitis@ariel.ucs.unimelb.edu.au Rania Horaitis Co-Ordinator HUGO Mutation Database Initiative Mutation Research Centre 7th Floor, Daly Wing St. Vincent's Hospital Victoria Parade Fitzroy, 3065 AUSTRALIA Ph: +61 3 9288 2980 Fax: +61 3 9288 2988 Email: horaitis@ariel.ucs.unimelb.edu.au Mutation Database website: http://ariel.ucs.unimelb.edu.au:80/~cotton/mut_database.htm From BIOSCI-REQUEST Wed Jan 21 22:01:45 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id WAA00718; Wed, 21 Jan 1998 22:01:45 -0800 Received: from muwayb.ucs.unimelb.EDU.AU (muwayb.ucs.unimelb.EDU.AU [128.250.20.7]) by net.bio.net (8.6.12-r-beta/8.6.6) with ESMTP id WAA00711; Wed, 21 Jan 1998 22:01:42 -0800 Received: from mur098 (stvpc05.medstv.unimelb.EDU.AU) by muwayb.ucs.unimelb.edu.au (PMDF V5.1-8 #24554) with SMTP id <01ISONWSTP9S000M8T@muwayb.ucs.unimelb.edu.au> for mutation@net.bio.net; Thu, 22 Jan 1998 17:00:48 +1100 Date: Thu, 22 Jan 1998 16:58:34 +0000 From: horaitis@ariel.ucs.unimelb.edu.au (Rania Horaitis) Subject: Newsletter number 2 X-Sender: horaitis@ariel.ucs.unimelb.edu.au To: mutation@net.bio.net Message-id: <01ISONWT0Z3M000M8T@muwayb.ucs.unimelb.edu.au> MIME-version: 1.0 X-Mailer: Windows Eudora Version 1.4.4 Content-type: text/plain; charset="us-ascii" X-Priority: 2 (High) HUGO MUTATION DATABASE INITIATIVE NEWSLETTER NO. 2, JANUARY, 1998 1. The 4th International Mutation Database Meeting was held in Baltimore in October 1997. There were 67 registrants and the meeting was very successful. The meeting program is available online on the website (see below). The attendees and abstracts will be available on the website soon. 2. For those who have not heard, there is a small HUGO Mutation Database meeting in Turin, Italy starting 3pm on March 27th 1998, adjoined to the Human Genome Meeting (HGM'98) 28-30 March 1998. This is mainly to present reports from the working groups and to elicit discussion. Those interested in attending should contact R. Horaitis. (horaitis@ariel.ucs.unimelb.edu.au) 3. An article entitled "Recommendations for a Nomenclature System for Human Gene Mutations" by S. Antonarakis and the contributions of the Nomenclature Working Group and wider community (in total 31 names) has just been published. Human Mutation Vol.11 #1 p1-3. This Human Mutation journal article will soon be available on-line through the publisher's Internet service Wiley InterScience. You may access Wiley InterScience through a link on the home page of the Human Mutation Web Site at "http://journals.wiley.com/humanmutation/" or by going directly to the Wiley InterScience site at http://www.interscience.wiley.com/." We are encouraging all locus specific databases to conform to these standards. 4. Charles Scriver, Piotr Nowacki and other members of the Content and Software Working Group, are currently preparing a Guidelines document describing how to set up in detail a locus specific database. This will be circulated and or posted for more general comment. 5. The campaign to encourage locus-specific databases has begun. Sixty-seven potential curators have been contacted so far, and we have had a positive response from twenty-seven. If you are interested in creating a database and curating for your gene of interest, and have not already indicated this to us, please contact us for further information. 6. Dr Matthias Wjst recently announced a new database for asthma and allergy linkages and mutations. This is available at "http://cooke.gsf.de". He is willing to share details and software with others wishing to set up a common disease/linkage database. For further details contact Dr Wjst (wjst@gsf.de). Finally, our initiative's community has now been finalised at ~350 and is growing. All communications with members have been documented and the interests and expertise of each member has been listed. Please advise us of any others you may think will be interested. Websites: HUGO-Mutation Database Initiative (HUGO-MDI): http://ariel.ucs.unimelb.edu.au:80/~cotton/mut_database.htm HUGO-MDI Mirror site: http://www2.ebi.ac.uk/mutations/ Rania Horaitis Co-Ordinator HUGO Mutation Database Initiative Mutation Research Centre 7th floor Daly Wing, St. Vincent's Hospital 41 Victoria Pde Fitzroy, VIC 3065 Australia Ph: 61-3-9288-2983 Fax: 61-3-9288-2988 Voicemail: 61-3-9288-2984 Email: horaitis@ariel.ucs.unimelb.edu.au Database website: http://ariel.ucs.unimelb.edu.au:80/~cotton/mut_database.htm From BIOSCI-REQUEST Thu Jan 22 12:54:48 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id MAA25366; Thu, 22 Jan 1998 12:54:48 -0800 Received: from post.cric.com (post.cric.com [192.138.207.103]) by net.bio.net (8.6.12-r-beta/8.6.6) with ESMTP id MAA25363; Thu, 22 Jan 1998 12:54:47 -0800 Received: from [206.34.154.120] (fitzgerald.cric.com) by post.genomecorp.com (PMDF V5.1-4 #15827) with ESMTP id <0EN7E0V8T007IY@post.genomecorp.com> for mutation@net.bio.net; Thu, 22 Jan 1998 15:53:19 -0500 (EST) Date: Thu, 22 Jan 1998 15:53:19 -0500 From: Michael FitzGerald Subject: subscribe To: mutation@net.bio.net Message-id: MIME-version: 1.0 Content-type: text/plain; charset="us-ascii" subscribe From BIOSCI-REQUEST Thu Jan 22 18:18:20 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id SAA07079; Thu, 22 Jan 1998 18:18:20 -0800 Received: from imo13.mx.aol.com (imo13.mx.aol.com [198.81.19.167]) by net.bio.net (8.6.12-r-beta/8.6.6) with ESMTP id SAA07075; Thu, 22 Jan 1998 18:18:19 -0800 From: PDM0312 Message-ID: <6451f76a.34c7f77d@aol.com> Date: Thu, 22 Jan 1998 20:50:50 EST To: mutation@net.bio.net Subject: Re: subscribe notice Content-type: text/plain; charset=US-ASCII Content-transfer-encoding: 7bit Organization: AOL (http://www.aol.com) X-Mailer: Inet_Mail_Out (IMOv11) Dear Curator: Somehow I continue to always be blind copied on these "subscribe" notices...please check and correct. Thanks. Patricia Murphy, Ph.D. Albany Medical College Albany, NY