From owner-mycology@net.bio.net Mon Nov 01 22:00:00 1993
Path: biosci!daresbury!daresbury!news
From: ibtulf@dk.dth.kbar (Ulf Thrane)
Newsgroups: bionet.mycology
Subject: properties of conidia
Message-ID: <1993Nov1.100805.16678@gserv1.dl.ac.uk>
Date: 1 Nov 93 09:00:46 GMT
Sender: ibtulf@kbar.dth.dk
Distribution: bionet
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Original-To: mycology@uk.ac.daresbury
Original-Cc: ibtulf@kbar.dth.dk


Hello everybody,
Please help me to find info on:

Surfacestructure, chemical composition, surface enzymes of
Aspergillus and Penicillium conidia and/or of ascospores from
their telemorphs.
Info concerning other genera of filamentous fungi will be
appreciated as well.

Please mail or fax any input to:
ibtulf@kbar.dth.dk    /   fax +45 4288 4922

Thanks,
Ulf Thrane 
(The mycology Group/Dept Biotechnology/Tech Univ/Lyngby/DK)

From owner-mycology@net.bio.net Tue Nov 02 22:00:00 1993
Path: biosci!daresbury!daresbury!news
From: PETER_PU@nl.tno.MBL.MBL-PROG
Newsgroups: bionet.mycology
Subject: luciferase reporter
Message-ID: <1993Nov3.125612.20682@gserv1.dl.ac.uk>
Date: 3 Nov 93 12:46:58 GMT
Sender: PETER_PU@mbl-prog.mbl.tno.nl
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Organization: Medical Biological Laboratory TNO
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X-Envelope-To: mycology@daresbury.AC.UK
Content-Identifier: luciferase re...
Precedence: first-class
Original-To: mycology@uk.ac.daresbury
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X-Mailer: Pegasus Mail v2.3 (R5).

For the analysis of promoters from not-efficiently transcribed
fungal genes we are considering the use of the firefly luciferase
reporter. We would be very pleased to obtain information of anyone
who ever gave this a try. Both good and bad experiences would be
appreciated. Also available luc-expression vectors for fungi would be
welcome.
Please send you messages to the newsgroup or directly to the
following E-mail address

Punt@mbl.tno.nl

Peter J. Punt, MBL/TNO, 2280 HV Rijswijk, The Netherlands

From owner-mycology@net.bio.net Tue Nov 02 22:00:00 1993
Path: biosci!daresbury!bioftp.unibas.ch!rc1!ub4b!mcsun!uknet!pipex!howland.reston.ans.net!math.ohio-state.edu!magnus.acs.ohio-state.edu!msosa
From: msosa@magnus.acs.ohio-state.edu (Mildred Sosa)
Newsgroups: bionet.mycology
Subject: Botrytis cinerea
Message-ID: <2b7a96$j6@charm.magnus.acs.ohio-state.edu>
Date: 3 Nov 93 03:58:30 GMT
Organization: The Ohio State University
Lines: 8
NNTP-Posting-Host: bottom.magnus.acs.ohio-state.edu

I need the classification of the fungus Botrytis cinerea, I already try some
mycology books but the information is not complete...I will appreciate some 
help.


Mildred

msosa@magnus.acs.ohio-state.edu

From owner-mycology@net.bio.net Wed Nov 03 22:00:00 1993
Path: biosci!barrnet.net!pacbell.com!ames!elroy.jpl.nasa.gov!sdd.hp.com!spool.mu.edu!umn.edu!puccini.crl.umn.edu!markh
From: markh@puccini.crl.umn.edu (Mark Hughes)
Newsgroups: bionet.jobs,bionet.plants,sci.bio,bionet.mycology,bionet.genome.arabidopsis
Subject: pre-doctoral fellowships available
Message-ID: <CFz5Fz.AxD@news.cis.umn.edu>
Date: 4 Nov 93 15:50:00 GMT
Sender: news@news.cis.umn.edu (Usenet News Administration)
Organization: United States Department of Agriculture
              Agricultural Research Station
              Cereal Rust Laboratory, St. Paul, MN
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Nntp-Posting-Host: puccini.crl.umn.edu

The Departments of Plant Pathology and Plant Biological Sciences at the 
University of Minnesota have USDA National Needs Fellowships available
to provide yearly stipends of 17,000 (plus the possibility of tuition
remission for three years) to highly qualified students.

The Graduate Program in the Department of Plant Pathology is directed
towards increasing resistance to fungal diseases in temperate cereals
through genetic engineering.  The Graduate Program in Plant Biological
Sciences is more general in nature and includes a variety of plant
biotechnology areas.

For more information on these two Graduate Programs at the University
of Minnesota contact:

		Professor Richard J. Zeyen
		Department of Plant Pathology
		University of Minnesota
		495 Borlaug Hall
		1991 Upper Buford Circle
		St Paul, MN  55108

		richz@puccini.crl.umn.edu 

From owner-mycology@net.bio.net Mon Nov 08 22:00:00 1993
Path: biosci!bcm!avdms8.msfc.nasa.gov!sol.ctr.columbia.edu!math.ohio-state.edu!wupost!wuecl.wustl.edu!news
From: FERRIS@WUSTLB (Patrick Ferris)
Newsgroups: bionet.mycology
Subject: Myxomycete
Message-ID: <2bn6pd$o9r@wuecl.wustl.edu>
Date: 9 Nov 93 04:37:01 GMT
Organization: WASHINGTON UNIVERSITY, BIOLOGY DEPT, ST LOUIS MO
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NNTP-Posting-Host: wustlb.wustl.edu
X-News-Reader: VMS NEWS v1.25

Several summers ago, while on vacation in Minnesota, I found
a big yellow plasmodium on a dead birch tree. I took part
of it, which soon turned into a large mass of spores. (Not like
P. polycephalum spores, though.) I still have them.
   Any myxomycologists out there who would be interested?

Patrick Ferris
Ferris@wustlb.wustl.edu

From owner-mycology@net.bio.net Wed Nov 10 22:00:00 1993
Path: biosci!daresbury!mrccrc!news.dcs.warwick.ac.uk!warwick!uknet!pipex!howland.reston.ans.net!usenet.ins.cwru.edu!cleveland.Freenet.Edu!do377
From: do377@cleveland.Freenet.Edu (Eric B Peterson)
Newsgroups: bionet.mycology
Subject: more pathogen/plant hormonal interactions?
Message-ID: <2bsqtc$bbs@usenet.INS.CWRU.Edu>
Date: 11 Nov 93 07:51:08 GMT
Organization: Case Western Reserve University, Cleveland, Ohio (USA)
Lines: 13
NNTP-Posting-Host: nextsun.ins.cwru.edu


Hello!  I am still looking for information/sources on hormonal 
interactions between fungal pathogens and plant hosts, especially
in cases where the hormone is produced by one and affects the
other.  Any information or advice would be greatly appreciated.
It is for a Plant Physiology term paper that I will probably
post here in about a month.
   Many thanks to all those who responded to my first request!
-- 
***Doing strange things in the name of art AND science...         
Eric B. Peterson (Ick)            :?  petersone@axe.humboldt.edu
895 7th Street                    :)  Ickster@garbo.uwasa.fi   
Arcata CA 95521    (707)822-0213  %|  do377@cleveland.freenet.edu

From owner-mycology@net.bio.net Wed Nov 10 22:00:00 1993
Path: biosci!daresbury!bioftp.unibas.ch!embl-heidelberg.de!uni-heidelberg!rz.uni-karlsruhe.de!xlink.net!sol.ctr.columbia.edu!
 howland.reston.ans.net!usenet.ins.cwru.edu!cleveland.Freenet.Edu!do377
From: do377@cleveland.Freenet.Edu (Eric B Peterson)
Newsgroups: bionet.mycology
Subject: C2H4 from pathogens for purpose of competition
Message-ID: <2bss40$dg5@usenet.INS.CWRU.Edu>
Date: 11 Nov 93 08:11:43 GMT
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     In my search for information on hormonal interactions 
between fungal pathogens and plant hosts I found some information
that I find rather interesting.  In the book Fungal Pathogenicity
and the Plant's Response, proceedings of a symposium held at Long
Ashton research Station, is a discussion between Professor
Stahmann and Dr. Beyersbergen of a fungus-plant patholgical
system where both produce ethylene.  The ethylene results in 
greater resistance of the plant to new infections, but the
established pathogen seems to keep ahead of the plant and is not
harmed. (oops, c. date of above book is 1973, symposium 1971)
     This looks to me as if it might be an interesting means 
for the fungus to get ahead in competition, reducing the chance
that another pathogen might also attack the plant.
     Does anyone have any information or opinions in this area?
-- 
***Doing strange things in the name of art AND science...         
Eric B. Peterson (Ick)            :?  petersone@axe.humboldt.edu
895 7th Street                    :)  Ickster@garbo.uwasa.fi   
Arcata CA 95521    (707)822-0213  %|  do377@cleveland.freenet.edu

From owner-mycology@net.bio.net Fri Nov 12 22:00:00 1993
Path: biosci!daresbury!mrccrc!news.dcs.warwick.ac.uk!warwick!pavo.csi.cam.ac.uk!pipex!howland.reston.ans.net!agate!darkstar.UCSC.EDU!nathan
From: nathan@cse.ucsc.edu (Nathan J. Wilson)
Newsgroups: bionet.mycology
Subject: Fungus fair nomenclature review
Message-ID: <2c1brt$nqo@darkstar.UCSC.EDU>
Date: 13 Nov 93 01:05:01 GMT
Organization: University of California, Santa Cruz (CE/CIS Boards)
Lines: 318
NNTP-Posting-Host: arapaho.cse.ucsc.edu

The science advisors of the Fungus Federation of Santa Cruz, Gregg
Ferguson and myself, are currently reviewing our use of taxonomic
nomenclature in preparation for our up coming Fungus Fair the weekend
of Jan 8 & 9.  I have included in this note a list of changes
currently under consideration, and the complete list of names used for
the species identified at the last fair.  We are actively seeking
advice on the appropriateness of the proposed changes as well as any
other changes that we should consider.  Personal opinions or
preferences are welcome, though we would appreciate any references you
can provide.

We would also like to start using author citations with our species
names.  Our principal reference is Arora's "Mushrooms Demystified"
which is sadly lacking in this information making this transition
difficult.  The current plan is to consult other reference books that
include this information and if the descriptions appear match those in
Arora, to adopt the author citation used.  I would also find a
detailed description of the meaning of such citations helpful.  For
example, from Miller's "Mushrooms of North America" what does '(Pers.
ex Fr.) Kummer' mean versus say 'Vitt. ex Fr.' or '(Fr.) Karst'?  I
understand that Fr. = Fries, Pers. = Persoon, and Vitt. = Vittadini,
but what are the parentheses and 'ex' (presumably latin for 'from')
all about?  Any advice on how to go about correctly using or finding
this information would be of help.

Thanks in advance,
   -------------------------    _________
         Nathan Wilson         <_________>
      nathan@cse.ucsc.edu          |_|        It is no dream!
      Co-Science Advisor          /___\    Matsutake are growing
   Minister of Local Forays        | |        On the belly of the mountain.
Fungus Federation of Santa Cruz    \_/ *83--                -Shigetaka


Changes considered and current plans:
Naematoloma to Hypholoma (plan to change):
Hypholoma aurantiaca
Hypholoma fasciculare

Lepiota to Leucoagaricus (change):
Leucoagaricus naucinus

Lepiota to Macrolepiota (*NO* change):
Macrolepiota rachodes

Leptonia to Entoloma (change):
Entoloma cyanea
Entoloma gracilipes
Entoloma parva

Boletus to Chalciporus (change):
Chalciporus piperatus

Boletus to Xerocomus (change):
Xerocomus chrysenteron
Xerocomus dryophilus
Xerocomus subtomentosus
Xerocomus truncatus

Boletus to Boletellus (don't know what to do with this one!)
Boletellus zelleri

Boletus to Pulveroboletus (*NO* change):
Pulveroboletus flaviporus

Orange yellow milked Lactarius:
Are the following species really distinct and how can you tell them apart 
macroscopically?
Lactarius chrysorheus (pileus not zonate)
Lactarius vinaceorufescens (pileus zonate, darker?)
Lactarius xanthogalactus (pileus zonate, lighter?)

Complete species from 1993 Santa Cruz Fungus Fair:
Agaricus albolutescens
Agaricus arvensis
Agaricus bernardii
Agaricus californicus
Agaricus campestris
Agaricus fuscovelatus
Agaricus hondensis
Agaricus lilaceps
Agaricus osecanus
Agaricus praeclaresquamosus
Agaricus semotus
Agaricus spp.
Agaricus subrutilescens
Agaricus xanthodermus
Agrocybe sp.
Albatrellus pescaprae
Aleuria aurantia
Aleuria rhenana
Amanita baccata
Amanita constricta
Amanita francheti
Amanita gemmata
Amanita magniverrucata
Amanita muscaria
Amanita ocreata
Amanita pachycolea
Amanita pantherina
Amanita phalloides
Amanita vaginata
Armillaria gallica
Armillaria mellea
Astraeus hygrometricus
Battarrea phalloides
Bolbitius vitellinus
Boletus amygdalinus
Boletus chrysenteron
Boletus dryophilus
Boletus edulis
Boletus flaviporus
Boletus piperatus
Boletus satanas
Boletus subtomentosus
Boletus truncatus
Boletus zelleri
Camarophyllus borealis
Camarophyllus pratensis
Camarophyllus russocoriaceus
Cantharellus cibarius
Cantharellus infundibuliformis
Cantharellus subalbidus
Caulorhiza umbonata
Chroogomphus ochraceus
Chroogomphus pseudovinicolor
Chroogomphus rutilus
Chroogomphus vinicolor
Clavariadelphus pistillaris
Clavariadelphus truncatus
Clavulina cristata
Clavulina rugosa
Clavulinopsis laeticolor
Clitocybe americana
Clitocybe brunneocephala
Clitocybe cyathiformis
Clitocybe dealbata
Clitocybe deceptiva
Clitocybe harperi
Clitocybe inversa
Clitocybe nebularis
Clitocybe nuda
Clitocybe sp.
Clitocybe subconnexa
Clitopilus prunulus
Collybia butyracea
Collybia dryophila
Collybia dryophila (gr.)
Collybia fuscopurpurea
Cortinarius calyptratus
Cortinarius cinnamomeus
Cortinarius cotoneus
Cortinarius cyanopus
Cortinarius infractus
Cortinarius luteoarmillatus
Cortinarius obtusus
Cortinarius phoeniceus
Cortinarius sodagnitus
Cortinarius spp.
Crepidotus mollis
Cystoderma fallax
Disciseda sp.
Entoloma madidum
Entoloma nidorosum
Entoloma rhodopolium
Flammulina velutipes
Fomitopsis pinicola
Geastrum pectinatum
Gomphidius glutinosus
Gomphidius smithii
Gomphidius subroseus
Gomphus floccosus
Gymnopilus luteocarneus
Gymnopilus sapineus
Gymnopilus spectabilis
Hebeloma crustuliniforme
Helvella crispa
Helvella lacunosa
Helvella macropus
Helvella maculata
Hericium abietis
Hydnum repandum
Hydnum umbilicatum
Hygrocybe acutoconica
Hygrocybe coccinea
Hygrocybe conica
Hygrocybe mollis
Hygrocybe psittacina
Hygrocybe punicea
Hygrophoropsis aurantiaca
Hygrophorus bakerensis
Hygrophorus eburneus
Hygrophorus flavodiscus
Hygrophorus hypothejus
Hygrophorus piceae
Hygrophorus pudorinus
Hygrophorus roseibrunneus
Hygrophorus russula
Hygrophorus sordidus
Hygrophorus subalpinus
Hypomyces chrysospermum
Inocybe geophylla
Inocybe lilacina
Inocybe pudica
Inocybe pyriodora
Inocybe sororia
Inocybe spp.
Inonotus tomentosus
Laccaria amethysteo-occidentalis
Laccaria laccata
Lactarius alnicola
Lactarius argillaceifolius
Lactarius chrysorheus
Lactarius deliciosus
Lactarius fragilis
Lactarius indigo
Lactarius rubrilacteus
Lactarius subviscidus
Lactarius vinaceorufescens
Lactarius xanthogalactus
Leccinum manzanitae
Lenzites betulina
Leotia viscosa
Lepiota clypeolaria
Lepiota flammeatincta
Lepiota naucina
Lepiota rachodes
Lepiota sp. "nabiscodisca"
Leptonia cyanea
Leptonia gracilipes
Leptonia parva
Leucopaxillus albissimus
Leucopaxillus amarus
Lycoperdon foetidum
Lycoperdon perlatum
Lyophyllum decastes
Marasmius plicatulus
Marasmius sp. (md2 206)
Melanoleuca melaleuca
Merulius tremellosus
Mycena acicula
Mycena alcalina
Mycena purpureofusca
Mycena sanguinolenta
Mycena spp.
Naematoloma aurantiaca
Naematoloma fasciculare
Nidula niveotomentosa
Omphalotus olivascens
Otidea alutacea
Otidea onotica
Panaeolus campanulatus
Paxillus involutus
Paxillus panuoides
Peziza sylvestris
Phaeolus schweinitzii
Phellodon atratus
Phlogiotis helvelloides
Pholiota sp.
Pholiota spumosa
Pholiota terrestris
Pholiota velaglutinosa
Phyllotopsis nidulans
Pleurotus ostreatus
Pluteus cervinus
Polyporus decurrens
Psathyrella hydrophila
Pseudohydnum gelatinosum
Psilocybe coprophila
Psilocybe cyanescens
Ramaria botrytis
Ramaria formosa
Ramaria gelatinosa
Ramaria myceliosa
Ramaria rasilispora
Ramaria(?) sp.
Rhizopogon ellenae
Rhizopogon ochraceorubens
Russula alutacea
Russula brevipes
Russula cremoricolor
Russula cyanoxantha
Russula integra
Russula placita
Russula rosacea
Russula silvicola
Russula sororia
Russula spp.
Russula subnigricans
Russula xerampelina
Sarcodon fuscoindicum
Sarcodon imbricatum
Schizophyllum commune
Sparassis crispa
Stereum hirsutum
Stropharia ambigua
Stropharia coronilla
Stropharia semiglobata
Suillus caerulescens
Suillus fuscotomentosus
Suillus ponderosus
Suillus pungens
Thelephora terrestris
Tremella foliacea
Tremella mesenterica
Trichaptum abietinus
Tricholoma flavovirens
Tricholoma imbricatum
Tricholoma magnivelare
Tricholoma pardinum
Tricholoma terreum
Tricholomopsis rutilans
Tubaria sp.
Tylopilus indecisus
Tyromyces chioneus
Vascellum pratense
Xeromphalina cauticinalis
Xylaria hypoxylon

From owner-mycology@net.bio.net Fri Nov 12 22:00:00 1993
Path: biosci!daresbury!bioftp.unibas.ch!rc1!ub4b!mcsun!howland.reston.ans.net!gatech!taco!chess.ncsu.edu!martenm
From: martenm@chess.ncsu.edu (Mark Marten)
Newsgroups: bionet.mycology
Subject: How to arrest cell growth/metabolism w/out changing morphology?
Message-ID: <1993Nov6.212314.22288@ncsu.edu>
Date: 6 Nov 93 21:23:14 GMT
Sender: news@ncsu.edu (USENET News System)
Reply-To: martenm@chess.ncsu.edu (Mark Marten)
Organization: NCSU Chem Eng
Lines: 19


I am working with a strain of Trichoderma reesei which produces 
large quantities of cellulase. I am interested in the rheology of
these suspensions and how cell concentration and morphology effects
rheology. 

Does anyone know of a way to arrest both growth and metabolism
while not effecting mycelial morphology? I would like to take samples 
from fermentations and analyze the rheology of these samples at a later
time.
--
--------------------------------------------------------------------------
Mark R. Marten       
Chemical Engineering
North Carolina State Univesity
Raleigh, NC 27695-7905
(919) 515-7176	
Internet: martenm@chess.ncsu.edu
-------------------------------------------------------------------------

From owner-mycology@net.bio.net Sun Nov 14 22:00:00 1993
Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!math.ohio-state.edu!magnus.acs.ohio-state.edu!usenet.ins.cwru.edu!gatech!news-feed-1.peachnet.edu!umn.edu!msc.edu!cdsmail!timbuk.cray.com!mmc.mmmg.com!horta!HYDE
From: hyde@horta
Newsgroups: bionet.mycology
Subject: Fatty Acid based taxomony of fungi
Message-ID: <1993Nov15.143303.27432@mail.mmmg.com>
Date: 15 Nov 93 14:33:03 GMT
Sender: news@mail.mmmg.com (Net News Administrator)
Reply-To: hyde@horta
Organization: 3M, St. Paul, MN
Lines: 11
Nntp-Posting-Host: horta.mmm.com

Has anyone out there been pursuing taxonomic research of fungi using
fatty acid chromatography? We have been using the MIDI system (Gas
chromatography-Fatty acid methyl ester) to begin developing
a mold ID library. We have been exploring some of the imperfect
fungi. What have you seen out there?

Fred Hyde				Internet: fwhyde@mmm.com
Corporate Research Laboratories		Fidonet: 1:282:31 or 1:282/75
3M Company, St. Paul, MN		Phone: (612) 736-5786 
_________________________________________________________________________
3M Company: The synonym for Innovation

From owner-mycology@net.bio.net Sun Nov 14 22:00:00 1993
Path: biosci!IASTATE.EDU!cbronson
From: cbronson@IASTATE.EDU (Charlotte R Bronson)
Newsgroups: bionet.mycology
Subject: (none)
Message-ID: <9311152237.AA16564@vincent1.iastate.edu>
Date: 15 Nov 93 22:37:50 GMT
Sender: daemon@net.bio.net
Distribution: bionet
Lines: 4

Please remove my name from the mycology user group.




From owner-mycology@net.bio.net Mon Nov 15 22:00:00 1993
Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!gatech!news-feed-1.peachnet.edu!umn.edu!news
From: brambl@graz.cbs.umn.edu (Robert Brambl)
Newsgroups: bionet.mycology
Subject: Forwarded message from Cheryl Grant
Message-ID: <CGLJ4J.Ixn@news2.cis.umn.edu>
Date: 16 Nov 93 17:50:59 GMT
Sender: news@news2.cis.umn.edu (Usenet News Administration)
Reply-To: brambl@molbio.cbs.umn.edu (Robert Brambl)
Organization: University of Minnesota, Twin Cities
Lines: 31
Nntp-Posting-Host: graz.cbs.umn.edu


                      Vacant Position


Department of Genetics
University of Melbourne
Parkville, Vic.  3052  Australia

Research Assistant


Applications are invited for the above position.  You should have a
degree in genetics, biochemistry or microbiology or related field. 
Experience with recombinant DNA techniques and/or protein chemistry
would be an advantage.  You would conduct studies into gene
regulation in fungi.  The position is available until 31 December
1994 in the first instance, and may be renewable for a further
period.


Salary:   $28,700 -  $38,950  (Research Assistant Grade 2)

Further information:   Professor Michael J. Hynes  (61 3) 344 6246, 
FAX (61 3) 344 5139

Applications close:   1 December 1993


Applications should be sent, quoting three referees (including
facsimile numbers) to The Director, Personnel Services, The
University of Melbourne, Parkville, Victoria  3052. 

From owner-mycology@net.bio.net Wed Nov 17 22:00:00 1993
Path: biosci!bcm!cs.utexas.edu!uunet!psinntp!newstand.syr.edu!rodan.syr.edu!griffin
From: griffin@rodan.syr.edu (David H. Griffin)
Newsgroups: bionet.mycology
Subject: Mitotic stability of transformants
Message-ID: <1993Nov18.122216.29617@newstand.syr.edu>
Date: 18 Nov 93 17:22:15 GMT
Organization: Syracuse University, Syracuse, NY
Lines: 19

Newsgroups: bionet.mycology
Subject: Mitotic stability of transformants
Summary: 
Followup-To: 
Distribution: world
Organization: Syracuse University, Syracuse, NY
Keywords:filamentous fungi, single and multiple transformants

I am looking for information on the relative mitotic stabilities of singly
integrated genes vs multiple integrants. Multiple integrants of either
multisite integrations or tandemly duplicated integrants are of
interest.Balance mentions this in his 1991 review, but cites no specific
data. Also, I am aware of Selker's work on RIP in N.crassa related to
meiotic instability. I am looking specifically for mitotic stability
problems.Does anyone know of any published data on differential
stabilities of these kinds of transformants, or
have any unpublished observations that they would be willing to share.
Thanks, David Griffin [e-mail griffin@mailbox.syr.edu
 

From owner-mycology@net.bio.net Wed Nov 17 22:00:00 1993
Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!spool.mu.edu!umn.edu!news
From: brambl@graz.cbs.umn.edu (Robert Brambl)
Newsgroups: bionet.mycology
Subject: Luciferase reporter system
Message-ID: <CGpooJ.HGo@news2.cis.umn.edu>
Date: 18 Nov 93 23:41:59 GMT
Sender: news@news2.cis.umn.edu (Usenet News Administration)
Reply-To: brambl@molbio.cbs.umn.edu (Robert Brambl)
Organization: University of Minnesota, Twin Cities
Lines: 22
Nntp-Posting-Host: graz.cbs.umn.edu

PREVIOUS POST FROM: Peter J. Punt
  
For the analysis of promoters from not-efficiently transcribed
fungal genes we are considering the use of the firefly luciferase
reporter. We would be very pleased to obtain information of anyone
who ever gave this a try. Both good and bad experiences would be
appreciated. Also available luc-expression vectors for fungi would be
welcome.
Please send you messages to the newsgroup or directly to the
following E-mail address

Punt@mbl.tno.nl

Peter J. Punt, MBL/TNO, 2280 HV Rijswijk, The Netherlands

+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++

FOLLOW UP FROM:  Robert Brambl

For bad experiences contact Van Gooch at goochv@caa.mrs.umn.edu.  He can  
also direct you to one or two other labs for whom this reporter has not  
worked. 

From owner-mycology@net.bio.net Thu Nov 18 22:00:00 1993
Path: biosci!news.cs.umb.edu!hsdndev!howland.reston.ans.net!pipex!uknet!glasgow!news
From: mark3@dcs.gla.ac.uk (Mark Magennis)
Newsgroups: bionet.mycology
Subject: Are wild mushrooms a radiation hazard in Europe?
Message-ID: <28044-0@goggins.dcs.gla.ac.uk>
Date: 19 Nov 93 12:26:21 GMT
Sender: news@dcs.glasgow.ac.uk (Network News)
Distribution: uk
Organization: Glasgow University Computing Science Dept.
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To: bionet.mycology@dcs.gla.ac.uk
Resent-From: usenet@dcs.gla.ac.uk
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X-Mailer: mail-news 3.1.3

I like to pick wild edible mushrooms in Scotland but a friend has suggested
that, since the Chernobyl nuclear accident, the radiation levels in wild
mushrooms are unsafe.

Not wanting to get cancer or stop eating delicious wild mushrooms, I need
some information about this. Anyone know about the radiation levels in
different parts of the UK and Europe?

Thanks, Mark

*************************************************************************
Mark Magennis                             email: mark3@dcs.glasgow.ac.uk
Dept. of Computing Science                  fax: (44) 041 330 4913
The University, Glasgow                     tel: (44) 041 339 8855 X8333
G12 8QQ  UK                                   
*************************************************************************
        ...and I say: "Cor baby, that's really free"

From owner-mycology@net.bio.net Sat Nov 20 22:00:00 1993
Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!ee.und.ac.za!hippo.ru.ac.za!g91l4660
From: g91l4660@hippo.ru.ac.za (Mr WD Leukes)
Newsgroups: bionet.mycology
Subject: lignin peroxidase assay
Message-ID: <CGuLMv.30A@hippo.ru.ac.za>
Date: 21 Nov 93 15:28:06 GMT
Organization: Rhodes University, Grahamstown, South Africa
Lines: 15

I have recently started trying to produce lignin peroxidase from
Phanerochaete chrysosporium in stationary culture, but with little
success. The culture and enzyme assay techniques seem to be quite
standard and easy, so perhaps there is some little trick that I am not
aware of. If anyone out there has any experience with this organism,
would you please contact me.

Thanks
Winston

-- 
          Winston Leukes - Department of Biochemistry and Microbiology
	  Rhodes University, P.O. Box 94,Grahamstown,6140,South Africa
	  Internet:g91l4660@hippo.ru.ac.za,Telephone:+27 (0461) 318441
	  Telefax: +27 (0461) 24377

From owner-mycology@net.bio.net Sun Nov 21 22:00:00 1993
Path: biosci!bloom-beacon.mit.edu!news.kei.com!sol.ctr.columbia.edu!howland.reston.ans.net!ee.und.ac.za!hippo.ru.ac.za!g91l4660
From: g91l4660@hippo.ru.ac.za (Mr WD Leukes)
Newsgroups: bionet.mycology
Subject: lignin peroxidase assay
Message-ID: <CGuLMv.30A@hippo.ru.ac.za>
Date: 21 Nov 93 15:28:06 GMT
Organization: Rhodes University, Grahamstown, South Africa
Lines: 15

I have recently started trying to produce lignin peroxidase from
Phanerochaete chrysosporium in stationary culture, but with little
success. The culture and enzyme assay techniques seem to be quite
standard and easy, so perhaps there is some little trick that I am not
aware of. If anyone out there has any experience with this organism,
would you please contact me.

Thanks
Winston

-- 
          Winston Leukes - Department of Biochemistry and Microbiology
	  Rhodes University, P.O. Box 94,Grahamstown,6140,South Africa
	  Internet:g91l4660@hippo.ru.ac.za,Telephone:+27 (0461) 318441
	  Telefax: +27 (0461) 24377

From owner-mycology@net.bio.net Mon Nov 22 22:00:00 1993
Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!math.fu-berlin.de!tertius.in-berlin.de!novell-russo.RZ-Berlin.MPG.DE!bruchez
From: bruchez@MPIMG-Berlin-Dahlem.MPG.DE (Jon Jules Paul Bruchez)
Newsgroups: bionet.mycology
Subject: FGN minireview correction
Summary: Mis-typing in intron consensus sequence
Keywords: FGN minireview regulatory sequences introns
Message-ID: <bruchez.2.000A4886@MPIMG-Berlin-Dahlem.MPG.DE>
Date: 23 Nov 93 18:16:55 GMT
Organization: Max-Planck-Institut fr Molekulare Genetik, Berlin.
Lines: 15
Nntp-Posting-Host: novell-russo.rz-berlin.mpg.de
Nntp-Posting-User: nntp_client
X-Newsreader: Trumpet for Windows [Version 1.0 Rev A]

I would like to apologise for a typing mistake on our part in preparation of 
the minireview "Regulatory sequences in the transcription of N. crassa genes: 
CAAT box, TATA box, Introns, Poly(A) tail formation sequences" in FGN (1993), 
40: 89-96.

On page 91 the 3' intron signal should read: 
                                      ...... ( C62 / T33 ) A100 G100 - G40

and NOT .....( T62 / C33) A100 G100 - G40.

We hope that this error has not caused too much confusion.

Jon Bruchez, J. Eberle and V. E. A. Russo.

Bruchez@MPIMG-BERLIN-DAHLEM.MPG.DE

From owner-mycology@net.bio.net Mon Nov 22 22:00:00 1993
Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!cs.utexas.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!robertson4.life.uiuc.edu!user
From: daniel@chulkn.chula.ac.th (Dan Guyot)
Newsgroups: bionet.mycology
Subject: Removing protists from NPV solution
Message-ID: <daniel-231193164542@robertson4.life.uiuc.edu>
Date: 23 Nov 93 22:43:11 GMT
Followup-To: bionet.mycology
Organization: University of Illinois at Urbana
Lines: 54
NNTP-Posting-Host: robertson4.life.uiuc.edu


Dear netters:
	One urgent question.
 
How can I eliminate microsporidia in my virus solution?  
 
I need an answer to this question by Wednesday morning (24 November 1993) 
in order to save the following experiment.
 
Experiment: Viability of Nuclear Polyhedrosis Virus excreted by two 
reptiles and an amphibian.
 
Objectives:
1) Determine the viability of excreted Nuclear Polyhedrosis Virus 
2) Estimate the quantity of virus ingested and excreted by the reptiles 
and amphibian.
 
	I fed caterpillars (Spodoptera. exigua) infected with NPV to a 
skink and a lizard and a toad.  However, I did not know that the 
caterpillars were also infected with a nasty microsporidian.  The feces 
from all three animals were collected and centrifuged to produce three 1 
ml solutions of NPV (one solution from each animal).  I want to bio-assay 
these three solutions to test the viability of the PV in them.  However, 
all threey solutions are contaminated with microsporidia (a protozoan).  
(This is an interesting finding in itself, it implies that both NPV and 
microsporidia can withstand the vagaries of a trip through the vertebrate 
digestive system.)  
	Nevertheless, I need to selectively get rid of the microsporidia 
in order test the viability of NPV with a bio-assay.  If I can separate 
NPV and microsporidia without damaging either then I can separately test 
the viability of each.
 
Possible solutions?	
 
1) use a selective anti-biotic against the microsporidia
2) pasteurize the solution and hope that one will be destroyed and the 
other won't
3) use some form of a gradient
	a) centrifuge?
	b) sucrose gradient?
	c) other?
 
 
Thanks in advance. BTW, please post answers directly to my e-mail address
below as I have no direct access to this group.
 
Daniel Guyot
35/1-2 Soi Aladin
Phahonyothin Rd. 
Bangkhen, Bangkok
10900
Thailand
 
e-mail: daniel@chulkn.chula.ac.th

From owner-mycology@net.bio.net Mon Nov 22 22:00:00 1993
Path: biosci!agate!howland.reston.ans.net!spool.mu.edu!umn.edu!puccini.crl.umn.edu!markh
From: markh@puccini.crl.umn.edu (Mark Hughes)
Newsgroups: bionet.jobs,bionet.plants,sci.bio,bionet.mycology,bionet.genome.arabidopsis
Subject: pre-doctoral fellowships available
Message-ID: <CFz5Fz.AxD@news.cis.umn.edu>
Date: 4 Nov 93 15:50:00 GMT
Sender: news@news.cis.umn.edu (Usenet News Administration)
Organization: United States Department of Agriculture
              Agricultural Research Station
              Cereal Rust Laboratory, St. Paul, MN
Lines: 22
Xref: biosci bionet.jobs:2772 bionet.plants:2097 sci.bio:7035 bionet.mycology:205 bionet.genome.arabidopsis:1662
Nntp-Posting-Host: puccini.crl.umn.edu

The Departments of Plant Pathology and Plant Biological Sciences at the 
University of Minnesota have USDA National Needs Fellowships available
to provide yearly stipends of 17,000 (plus the possibility of tuition
remission for three years) to highly qualified students.

The Graduate Program in the Department of Plant Pathology is directed
towards increasing resistance to fungal diseases in temperate cereals
through genetic engineering.  The Graduate Program in Plant Biological
Sciences is more general in nature and includes a variety of plant
biotechnology areas.

For more information on these two Graduate Programs at the University
of Minnesota contact:

		Professor Richard J. Zeyen
		Department of Plant Pathology
		University of Minnesota
		495 Borlaug Hall
		1991 Upper Buford Circle
		St Paul, MN  55108

		richz@puccini.crl.umn.edu 

From owner-mycology@net.bio.net Mon Nov 22 22:00:00 1993
Path: biosci!U.WASHINGTON.EDU!dstad
From: dstad@U.WASHINGTON.EDU (David Stadler)
Newsgroups: bionet.mycology
Subject: Postdoc opening
Message-ID: <Pine.3.87a.9311231401.A7263-0100000@carson.u.washington.edu>
Date: 23 Nov 93 22:57:01 GMT
Sender: daemon@net.bio.net
Distribution: bionet
Lines: 9

I have funds for a postdoc to work in our study of recombination and 
mutation at the mtr locus of Neurospora. The position is available 
immediately. The person need not have experience with Neurospora, but 
must have an interest in basic transmission genetics. The project 
involves both genetic and molecular analyses. I would appreciate any 
information about appropriate candidates who are available now or will be 
soon.
       David Stadler, Genetics SK-50, Univ. of Washington, Seattle


From owner-mycology@net.bio.net Tue Nov 23 22:00:00 1993
Path: biosci!BORCIM.WUSTL.EDU!goldman
From: goldman@BORCIM.WUSTL.EDU (Bill Goldman)
Newsgroups: bionet.mycology
Subject: (none)
Message-ID: <199311242304.AA18362@wugate.wustl.edu>
Date: 24 Nov 93 11:04:09 GMT
Sender: daemon@net.bio.net
Distribution: bionet
Lines: 4

I've heard that this is the electronic address for joining a Mycology
interest group on the network.  Please add me to the mailing list and tell
me how to access the posted information.  Thanks!


From owner-mycology@net.bio.net Tue Nov 23 22:00:00 1993
Path: biosci!daresbury!not-for-mail
From: mushex!vg@relay.NL.net (L.J.L.D. Van Griensven)
Newsgroups: bionet.mycology
Subject: Re: Are wild mushrooms a radiation hazard in Europe?
Message-ID: <2cv3jg$n3v@mserv1.dl.ac.uk>
Date: 24 Nov 93 07:48:00 GMT
Sender: daemon@mserv1.dl.ac.uk
Distribution: bionet
Lines: 21
Original-To: mark3@dcs.gla.ac.uk (Mark Magennis), mycology@dl.ac.uk

Hi

A collegue gave me the following reference concerning Radioactivity,  
Mushrooms and Chernobyl:

Vasser, Hrodzyns'ka and Lyuhin
(Kholodnyi Inst. Bot. Acad. Sci. Ukr., Kiev)
UKR BOT ZH 49(5): 79-86 1992.
In Ukr. with english summary.

Gamma spectral analysis of 39 species of wild and cultivated  
macromycetes in the territory of Ukranian Polessie.

( parts deleted etc etc.)
(about edible fungi:)
high levels of contamination prove a necessity to stop gathering and  
laying-in in the studied region.

Thus it's not advisable to collect mushrooms in the ukraine.

Leo

From owner-mycology@net.bio.net Tue Nov 23 22:00:00 1993
Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!olivea!gossip.pyramid.com!decwrl!decwrl!waikato!comp.vuw.ac.nz!news.massey.ac.nz!cc-server4.massey.ac.nz!DWatt
From: DWatt@massey.ac.nz (D.M. Watt)
Newsgroups: bionet.mycology
Subject: heterokaryon transformants
Keywords: heterokaryon, transformation, Aspergillus
Message-ID: <CGz19x.5FA@massey.ac.nz>
Date: 24 Nov 93 00:56:19 GMT
Organization: Massey University, Palmerston North, New Zealand
Lines: 9
X-Reader: NETNEWS/PC Version 2f

I am studying integrative transformation in filamentous fungi.
I am currently using the haploid, uracil requiring, Aspergillus nidulans 
strain 1-85, and a vector containing the N. crassa pyr4 gene. 
I would be interested to hear if anyone has found they have problems 
resolving their heterokaryon transformants. 
If you have found this to be an issue with transformants from any 
filamentous fungi, or selective marker, I would appreciate it if you 
would contact me. My email address is: D.M.Watt@massey.ac.nz
Thanks, Dianne. 

From owner-mycology@net.bio.net Tue Nov 23 22:00:00 1993
Path: biosci!CompuServe.COM!76440.316
From: 76440.316@CompuServe.COM ("Myco Pharmaceuticals, Inc.")
Newsgroups: bionet.mycology
Subject: Postdoctoral position
Message-ID: <931124125125_76440.316_EHC26-3@CompuServe.COM>
Date: 24 Nov 93 12:51:25 GMT
Sender: daemon@net.bio.net
Distribution: bionet
Lines: 17

We have an opening for a postdoctoral position for a period
of 3 years to study Multidrug Resistance in a fungal pathogen
of clinical importance. The candidate is expected to have astrong
background in yeast molecular genetics, to be highly motivated
and independent.

The position will be available as of January 1, 1994.

Those interested should send their CV and list of publications to:
Dr. Yigal Koltin
Myco Pharmaceuticals, Inc.
Bldg 300
One Kendall Square
Cambridge, MA 02139

For more information call:617-374-9090 ext226


From owner-mycology@net.bio.net Tue Nov 30 22:00:00 1993
Path: biosci!daresbury!not-for-mail
From: mushex!vg@relay.NL.net (L.J.L.D. Van Griensven)
Newsgroups: bionet.mycology
Subject: Re: Unusual hyphal branching
Message-ID: <2dich9$9hj@mserv1.dl.ac.uk>
Date: 1 Dec 93 15:16:57 GMT
Sender: daemon@mserv1.dl.ac.uk
Distribution: bionet
Lines: 48
Original-To: Richard Johnson <R.D.Johnson@massey.ac.nz>, mycology@dl.ac.uk,
 phorgen@credit.erin.utoronto.ca

Hi

Unusual hyphal branching is studied for edible mushrooms
by the unsiversity of toronto. Maybe you may try to contact Prof.  
Horgen at phorgen@credit.erin.utoronto.ca who may tell you more about  
his current research.

Leo van Griensven
Mushroom Experimental Station
Holland



Begin forwarded message:

Resent-From: server-daemon@dl.ac.uk
Date: Wed, 1 Dec 1993 02:01:30 GMT
Resent-Date: Wed, 1 Dec 93 0:0:20 UT
Resent-Message-Id: <CHC2yJ.A15@massey.ac.nz>
Precedence: list
From: Richard Johnson <R.D.Johnson@Massey.ac.nz>
Reply-To: Richard Johnson <R.D.Johnson@Massey.ac.nz>
Sender: "bionet.mycology mail newsgroup" <server-daemon@dl.ac.uk>
To: "bionet.mycology mail newsgroup" <bionet-news@dl.ac.uk>
Subject: Unusual hyphal branching
Comments: List problems/queries to <biosci@daresbury.ac.uk>
Comments: To mail both the group and netnews send to  
(mycology@dl.ac.uk)
X-Article-Number: bionet.mycology Msg # 195
X-Listpath: bionet-news
X-Mailer: MXT V 12.13.0

we have observed unusual hyphal branching within the host of  
Acremonium
lolii (an endophyte of ryegrass)  isolates which show variant growth
forms when grown in culture. This frequent branching, with odd
pertuberances is not observed in the parental strain which gave rise  
to
these variants. 


Has anyone observed a change in any plant/fungus interaction where  
the
fungal hyphae are either branching more than normal or they show odd
pertuberances.

thankyou,
                  Rich.

From owner-mycology@net.bio.net Tue Nov 30 22:00:00 1993
Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!wupost!waikato!comp.vuw.ac.nz!news.massey.ac.nz!cc-server4.massey.ac.nz!news
From: R.D.Johnson@Massey.ac.nz (Richard Johnson)
Newsgroups: bionet.mycology
Subject: Unusual hyphal branching
Message-ID: <CHC2yJ.A15@massey.ac.nz>
Date: 1 Dec 93 02:01:30 GMT
Sender: news@massey.ac.nz (USENET News System)
Organization: Massey University, New Zealand
Lines: 12
X-Xxdate: Wed, 1 Dec 93 15:03:06 GMT
X-Useragent: Nuntius v1.1.1d13

we have observed unusual hyphal branching within the host of Acremonium
lolii (an endophyte of ryegrass)  isolates which show variant growth
forms when grown in culture. This frequent branching, with odd
pertuberances is not observed in the parental strain which gave rise to
these variants. 

Has anyone observed a change in any plant/fungus interaction where the
fungal hyphae are either branching more than normal or they show odd
pertuberances.

thankyou,
                  Rich.

From owner-mycology@net.bio.net Tue Nov 30 22:00:00 1993
Path: biosci!daresbury!bioftp.unibas.ch!embl-heidelberg.de!uni-heidelberg!rz.uni-karlsruhe.de!xlink.net!howland.reston.ans.net!newsserver.jvnc.net!cyanamid!igate.cyanamid.com!sandy
From: sandy@nmr1.pt.cyanamid.COM (Sandy Silverman)
Newsgroups: bionet.mycology
Subject: Re: Unusual hyphal branching
Message-ID: <SANDY.93Dec1162737@nmr1.pt.cyanamid.COM>
Date: 1 Dec 93 21:27:37 GMT
References: <CHC2yJ.A15@massey.ac.nz>
Sender: news@cyanamid.uucp
Organization: American Cyanamid Company
Lines: 6
In-Reply-To: Richard Johnson's message of Wed, 1 Dec 1993 02:01:30 GMT

This could be a cell wall defect, perhaps glucan or chitin synthesis.
I can give you refs. if you would like to measure enzyme.
--
Sanford Silverman                      >Opinions expressed here are my own<
American Cyanamid  
sandy@pt.cyanamid.com, silvermans@pt.cyanamid.com     "Yeast is Best"

