From owner-mycology@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!daresbury!mrccrc!news.dcs.warwick.ac.uk!warwick!pipex!howland.reston.ans.net!europa.eng.gtefsd.com!emory!news-feed-2.peachnet.edu!news-feed-1.peachnet.edu!umn.edu!news From: brambl@graz.cbs.umn.edu (Robert Brambl) Newsgroups: bionet.mycology Subject: Re: Unusual hyphal branching Message-ID: Date: 2 Dec 93 00:01:54 GMT References: Sender: news@news2.cis.umn.edu (Usenet News Administration) Reply-To: brambl@molbio.cbs.umn.edu (Robert Brambl) Organization: University of Minnesota, Twin Cities Lines: 22 Nntp-Posting-Host: graz.cbs.umn.edu In article Richard Johnson writes: > we have observed unusual hyphal branching within the host of Acremonium > lolii (an endophyte of ryegrass) isolates which show variant growth > forms when grown in culture. This frequent branching, with odd > pertuberances is not observed in the parental strain which gave rise to > these variants. > > Has anyone observed a change in any plant/fungus interaction where the > fungal hyphae are either branching more than normal or they show odd > pertuberances. Many plant seed lectins (and presumably those in vascular tissues) will cause growth distortions in fungal hyphae, possibly because they disrupt the synthesis or deposition of the wall fabric. In some cases, multiple, adventitious branches appear, and in other cases the hyphal tips swell or become knobby. These effects can be reversed or blocked by the addition of low molecular weight sugar haptens of these lectins. Hope this helps. Bob From owner-mycology@net.bio.net Fri Dec 03 22:00:00 1993 Path: biosci!daresbury!not-for-mail From: mushex!vg@relay.NL.net (L.J.L.D. Van Griensven) Newsgroups: bionet.mycology Subject: Mycoplasms and Cluster formation in agaricus Message-ID: <2dn1vt$2oq@mserv1.dl.ac.uk> Date: 3 Dec 93 09:47:41 GMT Sender: daemon@mserv1.dl.ac.uk Distribution: bionet Lines: 20 Original-To: mycology@dl.ac.uk Hi My institute is currently involved in elucidating a new anomaly in mushroom farming: the formation of clusters. So: Is there any evidance for the presence of mycoplasms or lower fungi such as plasmodiophora to cause disease in higher basidiomycetes. Clustering is the anomalous growth of mushrooms in a big tight group consisting of 5 to 10 fruit bodies on a single clubroot like stipe. Any educated guesses about the possible cause of this clustering effect are gratefully invited. Leo van Griensven Mushroom Experimental Station Horst The Netherlands. From owner-mycology@net.bio.net Fri Dec 03 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!psinntp!newstand.syr.edu!rodan.syr.edu!griffin From: griffin@rodan.syr.edu (David H. Griffin) Newsgroups: bionet.mycology Subject: Mitotlic Instability of Transformants Message-ID: <1993Dec3.150026.11134@newstand.syr.edu> Date: 3 Dec 93 20:00:26 GMT Organization: Syracuse University, Syracuse, NY Lines: 130 I received several worthwhile responses to my inquiry on mitotic instability of transformants. I would like to thank all that replied. Below is a summary of the information that I obtained from these responses and some additional references I found as a result of this information. It seems that there is quite a variety of responses by individual fungi and several different levels of instability, indicating interesting variation in the mechanisms controlling recombination during mitotic growth. Keller N P, G C Bergstrom and O C Yoder. 1991. Mitotic stability of transforming DNA is deter;mined by its chromosomal configuration in the fungus Cochliobolus heterostrophus. Curr Genet 19:227-233. Cochliobolus heterostrophus was transformed with pH1S confering resistance to hyg B under the control of an 838 bp promoter fragment from C. heterostrophus. Plasmid integration was either at homologous sites (52% single copy, 33% tandemly repeated copies) or ectopic sites (4% single copy, 11% tandem repeats) resulting in four distinct configurations of integrated DNA. All four were stable during mitotic growth with no loss of integrated DNA after five subcultures on nonselective media or seven cycles of pathogenesis on maize. However, after eight or more passages on maize deletion of integrated DNA was detected with the frequency of deletion depending on the configuration of the integrated DNA. A single copy flanked by direct repeats of target, homologous-site sequences was least stable and a single copy at an ectopic site with no flanking repeats was most stable. Tandemly repeated copies at either homologous or ectopic sites had intermediate stabilities. Cytosine methylation occurred during mitotic growth, but had no effect on expression of hygB. Arnau J and R P Oliver. 1993. Inheritance and alteration of transforming DNA during an induced parasexual cycle in the imperfect fungus Cladosporium fulvum. Curr Genet 23:508-511. Protoplasts of a pAN7-1-transformed isolate of Cladosporium fulvum race 4, harboring a tandem duplication of the vector, and an untransformed race 5 isolate were fused, without selection for the presence of vector sequences. Fusion products were allowed to haploidize spontaneously. The inheritance of pAN7-1 sequences and the expression of the hph gene was studied in 85 progeny. A very high proportion (80%) of the progeny contained vector sequences and 70% of the progeny were resistant to hygromycin. Inactivation of the hph gene occurred in eight progeny. Rearrangement of vector sequences can account for the inactivation. There is no evidence for any RIP-like mechanism. Several novel bands were observed. Rikkerink, E.H.A., S.L. Solon, R. . Crowhurst, and M.D. Templeton. 1993. Integration of vectors by homologous recombination in the plant pathogen Glomerella cingulata. Current Genetics, In Press. In this paper we describe how transformants of G. cingulata, derived by homologous recombination of a circular vector with homologous gpdA sequences, appear to be quite unstable. Approximately 10-20% of the conidia derived from such transformants lose their hygromycin resistance by recombination between the (now repeated) homologous sequences, and wild type fragments are clearly visible in Southerns. These figures do not take into account any fluctuations due to founder effect, so they can't be taken as a true indication of the frequency but it is obviously high. Interestingly there may be some influence by the length of the repeated DNA since decreasing the length of repeated DNA from 1.5 kb to 0.5 kb resulted in a marked decrease in the frequency of this deletion event to the point where the deletion event is no longer detectable by Southerns. It should be kept in mind that we found a very high rate of homologous recombination at the gpdA locus in G. cingulata (95% of our transformants were homologous integrants) and that the unusually high frequency of homologous recombination in G.cingulata could be responsible for this effect. We haven't done any detailed studies of mitotic stability of multiple integration events. Klaas Swart (Dept. Genetics, Agricultural University, Wageningen) and coworkers studied mitotic stability of single and multiple integrants in Aspergillus niger transformants. Some are mentioned in: Debets, Swart, Holub, Goosen and Bos, 1990 Genetic analysis of amdS transformants of A.niger and their use in chromosome mapping. Mol Gen Genet 222: 284 - 290. Although he not worked on this recently, this is still a subject of interest in his lab. Additional references located referring to instability phenomena with integrated sequences: Berges T and C Barreau. 1991. Isolation of uridine auxotrophs from Trichoderma reesei and efficient transformation with the cloned ura3 and ura5 genes. Curr Genet 19:359-365. Blakemore E J A, M J Dobson, M J Hocart, J A Lucas and J F Peberdy. 1989. Transformation of Pseudocercosporella herpotrichoides using two heterologous genes. Curr Genet 16:177-180. Bussink H J D, J P T W van den Hombergh, P R L A van den IJssel and J Visser. 1992. Characterization of polygalacturonase-overproducing Aspergillus niger transformants. Appl Microbiol Biotechnol 37:324- 329. Faugeron G, L Rhounim and J L Rossignol. 1990. How does the cell count the number of ectopic copies of a gene in the premeiotic inactivation process acting in Ascobolus immersus? Genetics 124:585-591. Feher Z, M Schablik, A Kiss, A Zsindely and G Szabo. 1986. Characterization of inl+ transformants of Neurospora crassa obtained with a recombinant cosmid-pool. Curr Genet 11:131-137. Kistler H C and U K Benny. 1988. Genetic transformation of the fungal plant wilt pathogen, Fusarium oxysporum. Curr Genet 13:145-150. Pukkila P J and C Skrzynia. 1993. Frequent changes in the number of reiterated ribosomal RNA genes throughout the life cycle of the basidiomycete Coprinus cinereus. Genetics 133:203-211. Ruiz-Sala P, J A Perez-Gonzalez and D Ramon. 1993. Nucleotide sequence of a Trichoderma longibrachiatum DNA fragment encoding the 5.8S rRNA gene. Nucl Acids Res 21:741. Smith T L, J Gaskell, R M Berka, M Yang, D J Henner and D Cullen. 1990. The promoter of the glucoamylase-encoding gene of Aspergillus niger functions in Ustilago maydis. Gene 88:259-262. Unkles S E, E I Campbell, D Carrez, C Grieve, R Contreras, W Fiers, C A M J J Van den Hondel and J R Kinghorn. 1989. Transformation of Aspergillus niger with the homologous nitrate reductase gene. Gene 78:157-166. Upshall A. 1986. Genetic and molecular characterization of argB+ transformants of Aspergillus nidulans. Curr Genet 10:593-599. From owner-mycology@net.bio.net Fri Dec 03 22:00:00 1993 Path: biosci!daresbury!bioftp.unibas.ch!rc1!ub4b!EU.net!uunet!news.univie.ac.at!email.tuwien.ac.at!eichow!MOLBIO From: molbio@eichow (Martin Schindler) Newsgroups: bionet.mycology Subject: antibody ag. Hyg B Message-ID: <2dngil$hi@email.tuwien.ac.at> Date: 3 Dec 93 13:56:37 GMT Reply-To: molbio@eichow Organization: Dept. of Computergraphic, TU Vienna, Austria Lines: 32 NNTP-Posting-Host: eichow.tuwien.ac.at Hello netters!! I am working with Hygromycin B as a selectionmarker for filamemtous fungi and for some reasons I am interested in doing ELISAs with them. So here is the question: Is there an antibody aviable against E.coli Hygromycin B phosphtransferase (hph)? (the gene responsible for restistance against Hygromycin B (Gritz and Davies 1983, Gene 25:179-188)) If you know of such an antibody please inform me !! E-mail: molbio@eichow.tuwien.ac.at Greetings from Austria Martin =============================================================================== Mag. Martin Schindler Technical University of Vienna/Austria Inst. Biochem. Technol. und Mikrobiologie Abt. Mikrobielle Biochemie E-mail: molbio@eichow.tuwien.ac.at =============================================================================== =============================================================================== Mag. Martin Schindler Technical University of Vienna/Austria Inst. Biochem. Technol. und Mikrobiologie Abt. Mikrobielle Biochemie E-mail: molbio@eichow.tuwien.ac.at =============================================================================== From owner-mycology@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!MOOSE.UVM.EDU!lvaillan From: lvaillan@MOOSE.UVM.EDU (Lisa Vaillancourt) Newsgroups: bionet.mycology Subject: glucanase assays Message-ID: <9312071512.AA84375@moose.uvm.edu> Date: 7 Dec 93 16:23:53 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 9 Hello! I am wondering if anyone knows of a simple assay (preferably a colorimetric one) that I could use, preferably on intact tissue of Schizophyllum commune, to quantify the levels and activity of beta-glucanase enzymes? Thanks in advance for any help on this. Lisa Vaillancourt, Dept. Microbiology and Molecular Genetics, Stafford Building, University of Vermont, Burlington, VT. 05405 From owner-mycology@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!UKANVM.bitnet!FGSC From: FGSC@UKANVM.bitnet (Craig Wilson) Newsgroups: bionet.mycology Subject: FGN - Call for contributions (41); Correction (39) Message-ID: <9312071803.AA23942@net.bio.net> Date: 7 Dec 93 18:00:44 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 29 FUNGAL GENETICS NEWSLETTER 41 (1994) CALL FOR CONTRIBUTIONS FGN #41 will be published in the summer of 1994, and contributions will be accepted until March 1, 1994. The Newsletter is a suitable place for short research notes, linkage data, information concerning new mutants, announcements of meetings, or of positions available et cetera. It is a policy not to accept an abstract or shorter version of a full-length paper which will be published soon in a regular journal. However, a more extensive description of techniques or mutants used in a regular paper would be appropriate for FGN. A set of instructions for contributors can be found on page 5 of FGN 40. Contributions should be sent to: Dr. Peter J. Russell, FGN Editor, Department of Biology, Reed College, Portland, Oregon 97202, USA. E-mail to yeast@reed.edu CORRECTION TO FGN 39, 1992 The following correction to an article in FGN 39 was omitted from the appropriate section of FGN 40: In the article by Haedo, Mautino and Rosa (pg 23) the last sentence of the first paragraph should read "Table I shows that the analyzed region on chromosome I is largely NON-Oak Ridge both in multicent-2 and, as expected, the Mauriceville-1c genome." From owner-mycology@net.bio.net Tue Dec 07 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!europa.eng.gtefsd.com!howland.reston.ans.net!vixen.cso.uiuc.edu!gardner2.life.uiuc.edu!user From: erik_read@qms1.life.uiuc.edu (Erik Read) Newsgroups: bionet.mycology Subject: Chloroform....... Message-ID: Date: 8 Dec 93 04:29:11 GMT Followup-To: bionet.mycology Organization: Only when Necessary Lines: 8 NNTP-Posting-Host: gardner2.life.uiuc.edu Does anyone know of any Fungus that is resistant (or tolerant) to chloroform ? (((((((((((((((((((((((((((((((((((((((((((((((((((((((((((((((((((((((( )Hey you, Antero Vipunen, rise from sleeping, from dreaming too much ! ) ( There's not a man in the departed...my mound of flesh already rotted!( )Erik_Read@qms1.life.uiuc.edu -The Kalevala ) (((((((((((((((((((((((((((((((((((((((((((((((((((((((((((((((((((((((( From owner-mycology@net.bio.net Wed Dec 08 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!decwrl!decwrl!waikato!canterbury.ac.nz!otago.ac.nz!sanger.otago.ac.nz!biocmag From: biocmag@sanger.otago.ac.nz (Michael Gieseg) Newsgroups: bionet.mycology Subject: yeast electroporation Message-ID: Date: 9 Dec 93 01:43:10 GMT Sender: usenet@news.otago.ac.nz (News stuff) Organization: University of Otago Lines: 12 Nntp-Posting-Host: sanger.otago.ac.nz X-Newsreader: TIN [version 1.1 PL8] Does anybody out there have protocols for the electroporation of Saccharomyces cerevisiae and Candida albicans. I know they exist and if some kind person could Email them to me that would be great. Could someone also please tell me the addresses of archive sites containing methods and protocols for Mol-biol techniques. Thanks Heaps Mike Gieseg University of Otago New Zealand Email: biocmag@sanger.otago.ac.nz From owner-mycology@net.bio.net Wed Dec 08 22:00:00 1993 Path: biosci!MOLBIO.CBS.UMN.EDU!ptm From: ptm@MOLBIO.CBS.UMN.EDU ("Paul T. Magee") Newsgroups: bionet.mycology Subject: electroporation of Candida albicans Message-ID: <9312091517.AA22906@molbio.cbs.umn.edu> Date: 9 Dec 93 14:17:54 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 4 --We tried for two months to electroporate Canidida albicans.We varied buffer, temperature, growth state, and all the electric parameters. We got no reproduc-ible results. Pete Magee From owner-mycology@net.bio.net Wed Dec 08 22:00:00 1993 Path: biosci!barrnet.net!nntp.crl.com!nntp!harris From: harris@bhc.com (Bob Harris) Newsgroups: bionet.mycology Subject: Sporeprint news from Mushroompeople? Message-ID: Date: 9 Dec 93 04:12:54 GMT Organization: Bob Harris Consulting Lines: 8 NNTP-Posting-Host: bhc.com X-Newsreader: VersaTerm Link v1.1.3 Several Years ago Mushroompeople had a newsletter Called Sporeprint News. It ran about 4 issues. I am seeking copies of issues 2, 3, and 4 if anyone has them, please email me at harris@bhc.com Bob Harris 8 :-) From owner-mycology@net.bio.net Thu Dec 09 22:00:00 1993 Path: biosci!bcm!cs.utexas.edu!uunet!decwrl!decwrl!usenet.coe.montana.edu!netnews.nwnet.net!ns1.nodak.edu!plains!alwhite From: alwhite@plains.NoDak.edu (Alan R White) Newsgroups: bionet.mycology Subject: Announce: CELLWALL Plant & Fungal Cell Wall Discussion Message-ID: Date: 10 Dec 93 01:44:07 GMT Sender: usenet@ns1.nodak.edu (Usenet login) Organization: North Dakota Higher Education Computing Network Lines: 47 Nntp-Posting-Host: plains.nodak.edu X-Newsreader: TIN [version 1.2 PL1] Electronic Discussion Group for Plant and Fungal Cell Wall Research Seeks Participants An electronic discussion group has been established on a listserv server at North Dakota State University, USA, E-Mail listserv@vm1.nodak.edu The listserv mailing list discussion group has been set up as a freely accessible electronic communication forum for use by anyone interested in cell wall research. The scope of this discussion group will be all aspects of research into the biology of plant and fungal cell walls including: structure; polysaccharides, proteins and other components; synthesis; expansion; and regulation. Requests for help on specific topics, requests for samples or standards, and suggestions on laboratory techniques may be posted to the list. The list may also be used for announcements of meetings, calls for papers or posters, posting of abstracts from recent meetings or recently published papers, or announcements of visits by scientists to particular areas. To subscribe to the list send an E-Mail message to: listserv@vm1.nodak.edu With the body of the message containing the command: sub cellwall Please use only the above command. Leave the subject line blank and do not type any other message or text. All mailing to the cellwall list should be sent to: cellwall@vm1.nodak.edu Please pass this information on to other interested colleagues. For more information send E-Mail to: alwhite@plains.nodak.edu Alan White Department of Botany North Dakota State University Fargo, ND 58105-5517 USA Phone: (701) 237-8380 Fax: (701) 237-7149 From owner-mycology@net.bio.net Thu Dec 09 22:00:00 1993 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!pipex!uknet!EU.net!sun4nl!wau.nl!VLINDERS@rcl.wau.nl From: vlinders@rcl.wau.nl (meindert de jong <^_^>) Newsgroups: bionet.mycology Subject: Fungal Palynomorphs (4 screens) Message-ID: Date: 10 Dec 93 15:16:04 GMT Sender: news@news.wau.nl (News Administrator) Reply-To: vlinders@rcl.wau.nl Organization: Wageningen Agricultural University Lines: 76 Fungal palynomorphs were noticed in the earliest of microsco- pical studies. They were generally ignored until the turn of this century. Unlike pollen and other spores, there was a long hiatus until the 1950s, when the first useful taxonomic treat- ment began. Morphology The size of a fungal palynomorph can range from larger than an actinomycete to as large as fi=osiil seed coats (2 to 1000 mu). There is a very extreme range of general morphology, from simple spheres to complex multicellular bodies. Fungal spores are colorless to some shade of brown. The pigemnt is generally melanin. Ubiquitous Nature Fungi have a fossil record in time and space more or less equal to the algae. They are recorded from Precambian strata. Fungal palynomorphs are found most commonly in Jurassic to Recent sediments. The development of the Ascomycetes, an advanced group of fungi, is believed to have been parallel to the development of the Angiosperms. Fungal palynomorphs per- sist after many other palynomorphs have been degradaded. Occurrence Petroleum/Coal exploration: Geological horizons can be traced by looking for characteristic fungal palynomorphs. Stumbling blocks: As with most palynomorphs, fungal remains can be reworked into younger sediment. This can result in erronous interpretations of stratigraphy and paleoclimate. Archaological implications; Fungi are among the most resistant of the microfossil clues. Paleozoic fungal spores often remain after the coking process. Elsik, W.C. (1974). Nothofagus in North America. Pollen et Spores, 16: 285-299. Pollen grains belonging to the genus Nothofagus from Eocene and Oligocene sediments of the Gulf Coastal Plin and the Pacific Northwest have been studied. A new species, Nothofagus tschudgi is described on the basis of the Eocene sediments. Occurrence of thin granules and bacula typical of the Nothofa- gus pollen grains has been demonstrated with the help of the scanning electron microscope. {with some amazing phase contrast and SEM photographs of Nothofagus spp. holotypes and types; paper published with permission of EXXON Company, Houston, Texas} Elsik, W.C. (1989). The Fungal Monotype Felixites N. Gen. Pollen et Spores 31: 155-159. Felexites n. gen. is described for late Paleozoic fungal didymospores that are aporate and characterized by a very thick median septum. The form genus is comprised of two spe- cies, Felixites pollenisimilus and Felixites playfordii. Some other papers of William C. Elsik: Palynology of a late Pleistocene giant ground sloth locality, southwest Harris county, Texas. Pollen & Spores 1986. Late Neogene palynomorph diagrams, northern Gulf of Mexico. Trans. Gulf Coast Assoc. of Geolog. Soc. 1969. {remarkable pollen grains, "microforams", neogene plant tissues etc. of spruce, Ambrosia, Helianthus, Alnus.} Fungal palynomorphs recovered from recent river deposits, Luangwa Valley, Zambia. Palynology 10: 35-60. William Elsik [The MycoStrat Connection, 12410 Stafford Springs Dr., 1Hous- ton, TX 77077] is currently writing a book and may include a little figure of mine about dispersal of a modern fungus. =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Sincerely Yours, dr Meindert D. de Jong | seeking a E-mail: Vlinders@RCL.WAU.NL | telejob Voice: (+31) 8370 21937 FAX: (+31) 8370 23110 Photo:de Jong e.a.'90. Risk Analysis for Biological Control. About biocontrol of a forest weed Plant Disease 74: 189 de Jong'92 Letter to Editor'92. Risk Assessment for Biological Control of Forest Weed by Common Fungus. Risk Analysis 12,465 From owner-mycology@net.bio.net Thu Dec 09 22:00:00 1993 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!torn!csd.unb.ca!UNBSJ.CA!c0hx From: c0hx@UNBSJ.CA (JASON K. DOBRANIC) Newsgroups: bionet.mycology Subject: Endophytologists Message-ID: Date: 10 Dec 93 18:21:25 GMT Sender: news@jupiter.sun.csd.unb.ca Organization: UNB Saint John Campus Lines: 2 Hi I was wondering if there are any Endophytologist on this Discussion Group? From owner-mycology@net.bio.net Sun Dec 12 22:00:00 1993 Newsgroups: bionet.mycology Path: biosci!bloom-beacon.mit.edu!news.kei.com!eff!usenet.ins.cwru.edu!agate!ames!decwrl!decwrl!waikato!comp.vuw.ac.nz!news.massey.ac.nz!cc-server4.massey.ac.nz!news From: Richard Johnson Subject: Re: Chloroform....... Message-ID: X-Xxdate: Mon, 13 Dec 93 10:26:31 GMT Sender: news@massey.ac.nz (USENET News System) Organization: Massey University, New Zealand X-Useragent: Nuntius v1.1.1d13 References: Date: Sun, 12 Dec 1993 21:24:33 GMT Lines: 5 I dont know what it is but this very morning I discovered something growing in my chloroform! It looks fungal to me but I wouldnt like to hasard a guess as to what group it belongs to. I must say, whatever it is it must be a tough little beasty. From owner-mycology@net.bio.net Sun Dec 12 22:00:00 1993 Newsgroups: bionet.mycology Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!news.univie.ac.at!edvz.sbg.ac.at!dpfl03!zorer From: zorer@edvz.sbg.ac.at (Roberto Zorer) Subject: test1 Message-ID: Lines: 1 Sender: news@wst.edvz.sbg.ac.at (USENET News System) Organization: University of Salzburg X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Date: Mon, 13 Dec 1993 17:36:04 GMT Dies ist ein Test. From owner-mycology@net.bio.net Sun Dec 12 22:00:00 1993 Newsgroups: bionet.mycology Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!news.univie.ac.at!edvz.sbg.ac.at!dpfl03!tutor1 From: tutor1@edvz.sbg.ac.at (Richard Egger) Subject: wanted Message-ID: Lines: 1 Sender: news@wst.edvz.sbg.ac.at (USENET News System) Organization: University of Salzburg X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Date: Mon, 13 Dec 1993 18:09:33 GMT test From owner-mycology@net.bio.net Sun Dec 12 22:00:00 1993 Newsgroups: bionet.mycology Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!news.univie.ac.at!edvz.sbg.ac.at!dpfl03!zorer From: zorer@edvz.sbg.ac.at (Roberto Zorer) Subject: test Message-ID: Lines: 2 Sender: news@wst.edvz.sbg.ac.at (USENET News System) Organization: University of Salzburg X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Date: Mon, 13 Dec 1993 17:56:49 GMT Dies ist ein test. From owner-mycology@net.bio.net Mon Dec 13 22:00:00 1993 Path: biosci!UKANVM.bitnet!FGSC From: FGSC@UKANVM.bitnet (Craig Wilson) Newsgroups: bionet.mycology Subject: Novozyme reports Date: 13 Dec 1993 15:52:42 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <9312132352.AA29714@net.bio.net> We periodically get questions about availability and quality of Novozyme. It seems lot-to-lot variability of the product remains high. We heard today from Lisa Vaillancourt at the U of Vermont that one shipment from Cal Biochem was of low quality and another worked well. A call to another supplier (see below) yielded a report that the lot they currently handle (PPM 4356) is working well, according to information they have received from customers. This supplier's address is: InterSpex Products Inc. 11558 Chess Dr. , Suite 114 Foster City, CA 94404 Phone 415-570-4337 FAX 415-570-5215 With the high concentration of Novozyme users reading this group, and the variability of the product, it would be a useful exercise to report here whatever experience you have recently had with Novozyme. Suppliers and numbers of lots both good and bad should be information read with interest by many people.  From owner-mycology@net.bio.net Mon Dec 13 22:00:00 1993 Path: biosci!ZEUS.TAMU.EDU!dje0282 From: dje0282@ZEUS.TAMU.EDU (Dan Ebbole) Newsgroups: bionet.mycology Subject: Novozyme Date: 14 Dec 1993 06:15:36 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <9312141415.AA01214@net.bio.net> NNTP-Posting-Host: net.bio.net We have been using Interspex supplied Novozyme with good results. However, it seems to be very active: our normal protocol is for around 60 min treatment of germinated N. crassa conidia for protoplasting. This batch gives complete protoplasting within 20 min, a 60 min digest yields protoplasts with low transformability. ------------------------ Dr. Daniel Ebbole dje0282@summa.tamu.edu Dept. of Plant Pathology and Microbiology Texas A&M University College Station, TX 77843-2132 From owner-mycology@net.bio.net Mon Dec 13 22:00:00 1993 Path: biosci!biowri.edu.ar!cabral From: cabral@biowri.edu.ar (cabral) Newsgroups: bionet.mycology Subject: RE: Endophytologist Date: 13 Dec 1993 16:57:54 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <026lm673@biowri.edu.ar> Dear Jason K. Dobranic: I am not if I am a Endophytologist, but I have been working during the last 4 years on the endophytes of native grasses of Argentina. I am very interested in contact people who work in diferents aspects of endophytes research. Best wishes, Daniel Daniel Cabral (cabral@biowri.edu.ar) From owner-mycology@net.bio.net Mon Dec 13 22:00:00 1993 Newsgroups: bionet.mycology Path: biosci!bcm!cs.utexas.edu!uunet!olivea!decwrl!decwrl!netcomsv!netcom.com!czs From: czs@netcom.com (Christian Smith) Subject: Mycologist/Lichenologist database Message-ID: Organization: NETCOM On-line Communication Services (408 241-9760 guest) Date: Tue, 14 Dec 1993 23:18:36 GMT Lines: 4 Sometime back there was some discussion of the afore mentioned database. Can anyone give me any info on it. Thanks in advance. Christian From owner-mycology@net.bio.net Mon Dec 13 22:00:00 1993 Newsgroups: bionet.mycology Path: biosci!bcm!cs.utexas.edu!swrinde!elroy.jpl.nasa.gov!usc!wupost!waikato!comp.vuw.ac.nz!news.massey.ac.nz!cc-server4.massey.ac.nz!news From: Richard Johnson Subject: Re: Endophytologists Message-ID: X-Xxdate: Wed, 15 Dec 93 09:26:39 GMT Sender: news@massey.ac.nz (USENET News System) Organization: Massey University, New Zealand X-Useragent: Nuntius v1.1.1d13 References: Date: Tue, 14 Dec 1993 20:24:46 GMT Lines: 4 We work on endophytes of New Zealand ryegrass here at Massey University, Palmerston nth, New Zealand. Richard From owner-mycology@net.bio.net Tue Dec 14 22:00:00 1993 Newsgroups: bionet.mycology Path: biosci!bcm!cs.utexas.edu!wupost!waikato!canterbury.ac.nz!otago.ac.nz!sanger.otago.ac.nz!biocmag From: biocmag@sanger.otago.ac.nz (Michael Gieseg) Subject: thanks for electro help Message-ID: Sender: usenet@news.otago.ac.nz (News stuff) Nntp-Posting-Host: sanger.otago.ac.nz Organization: University of Otago X-Newsreader: TIN [version 1.1 PL8] Date: Wed, 15 Dec 1993 23:15:10 GMT Lines: 7 Gidday Thanks to all those people how replyed to my request for help on yeast electroporation, I now have plenty to go on with. Mike From owner-mycology@net.bio.net Tue Dec 14 22:00:00 1993 Path: biosci!DARTMOUTH.EDU!Deborah.B.Pedersen From: Deborah.B.Pedersen@DARTMOUTH.EDU (Deborah B. Pedersen) Newsgroups: bionet.mycology Subject: Neurospora b-gal activity mutant Date: 15 Dec 1993 12:02:08 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 4 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <8166984@prancer.Dartmouth.EDU> NNTP-Posting-Host: net.bio.net I am in need of a Neurospora crassa mutant strain which lacks endogenous "b-galactosidase" activity. Does anyone out there have such a strain, or know of any reason why I would not be able to generate one? Thanks in advance! Deborah Pedersen From owner-mycology@net.bio.net Tue Dec 14 22:00:00 1993 Newsgroups: bionet.mycology Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!europa.eng.gtefsd.com!emory!news-feed-1.peachnet.edu!umn.edu!news From: brambl@graz.cbs.umn.edu (Robert Brambl) Subject: Re: Russian Mycologists Message-ID: Sender: news@news.cis.umn.edu (Usenet News Administration) Nntp-Posting-Host: graz.cbs.umn.edu Reply-To: brambl@molbio.cbs.umn.edu (Robert Brambl) Organization: University of Minnesota, Twin Cities References: <199312150157.AA28692@ua.d.umn.edu> Distribution: bionet Date: Wed, 15 Dec 1993 18:41:30 GMT Lines: 18 In article <199312150157.AA28692@ua.d.umn.edu> grad@UA.D.UMN.EDU (stephen hedman) writes: > I am interested in making contact with Russian mycologists in either > Moscow or Petrozavodsk, especially if they might have interests in > Neurospora. Names and email addresses should be sent to > grad@ua.d.umn.edu. > > Thanks, steve Steve: Try Mikhail S. Kritsky, INBIO@GLAS.APC.ORG, who recently returned to Moscow from a study visit to Spain. Would Russian Neurosporologists working in Minneapolis be of interest to you? Bob From owner-mycology@net.bio.net Tue Dec 14 22:00:00 1993 Path: biosci!cornell.edu!RPK1 From: RPK1@cornell.edu (Dick Korf) Newsgroups: bionet.mycology Subject: Request for addition to user group Date: 15 Dec 1993 09:42:38 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <199312151739.AA06214@postoffice2.mail.cornell.edu> NNTP-Posting-Host: net.bio.net Would appreciate being added to your user group, as sufggested by Dennis M. Dixon. Richard P. Korf, RPK1@cornell.edu __________________________________________________ Richard P. Korf: tel: Cornell Univ., voice 607/255-3292, fax 607/255-4471 Mycotaxon: voice 607/273-4357, fax (WAIT for message) 607/273-4357 From owner-mycology@net.bio.net Tue Dec 14 22:00:00 1993 Path: biosci!UA.D.UMN.EDU!grad From: grad@UA.D.UMN.EDU (stephen hedman) Newsgroups: bionet.mycology Subject: Russian Mycologists Date: 14 Dec 1993 17:57:40 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 6 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <199312150157.AA28692@ua.d.umn.edu> NNTP-Posting-Host: net.bio.net I am interested in making contact with Russian mycologists in either Moscow or Petrozavodsk, especially if they might have interests in Neurospora. Names and email addresses should be sent to grad@ua.d.umn.edu. Thanks, steve From owner-mycology@net.bio.net Wed Dec 15 22:00:00 1993 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!sol.ctr.columbia.edu!news.kei.com!newsstand.cit.cornell.edu!newsstand.cit.cornell.edu!usenet From: Mycologist Newsgroups: bionet.mycology Subject: Re: Mycologist/Lichenologist database Date: 16 Dec 1993 15:45:36 GMT Organization: Cornell University Lines: 8 Sender: rpk1@cornell.edu (Verified) Distribution: world Message-ID: <2epvr0INNc1u@newsstand.cit.cornell.edu> References: NNTP-Posting-Host: 128.253.146.121 X-UserAgent: Nuntius v1.1.1d7 X-XXDate: Thu, 16 Dec 93 10:47:29 GMT >To: czs@netcom.com (Christian Smith) There is a database called MYCOLOGISTS ONLINE, listing the e-mail addresses of mycologists and lichenologists. If that's what you are interested in, the list is planned to go online via the Harvard University gopher, but meanwhile you can get a copy by e-mailing Dr. Pavel Lizon at PL18@cornell edu From owner-mycology@net.bio.net Thu Dec 16 22:00:00 1993 Path: biosci!rutgers!uwm.edu!news.moneng.mei.com!howland.reston.ans.net!spool.mu.edu!sdd.hp.com!saimiri.primate.wisc.edu!mimbres.cs.unm.edu!moe.ksu.ksu.edu!osuunx.ucc.okstate.edu!mwg4265 From: mwg4265@osuunx.ucc.okstate.edu (Gates) Newsgroups: bionet.mycology Subject: KUDZU!! Message-ID: <1993Dec16.040557.27453@osuunx.ucc.okstate.edu> Date: 16 Dec 93 04:05:57 GMT Distribution: bionet.mycology Organization: Oklahoma State University Computer Center Lines: 10 I have a question that has interested me since hearing a talk on a related topic about Cercospora(sp?) sp. fungi being used to control water hyacinth in Florida. I was told that researchers are now trying inversions to keep pathogenic fungi moist in order to control kudzu in the Southeast. What species of fungi are they using? Please e-mail. Thanks! Gates mwg4265@osuunx.occ.okstate.edu From owner-mycology@net.bio.net Thu Dec 16 22:00:00 1993 Path: biosci!myconet.org!christian.smith From: christian.smith@myconet.org Newsgroups: bionet.mycology Subject: TEST POSTING Date: 17 Dec 1993 08:28:52 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <9312170813.A0775wk@myconet.org> NNTP-Posting-Host: net.bio.net Just seeing how things work. From owner-mycology@net.bio.net Tue Dec 21 22:00:00 1993 Newsgroups: bionet.mycology Path: biosci!daresbury!keele!uknet!pipex!howland.reston.ans.net!spool.mu.edu!olivea!decwrl!decwrl!netcomsv!netcom.com!czs From: czs@netcom.com (Christian Smith) Subject: Mycological Discussion Groups Message-ID: Organization: NETCOM On-line Communication Services (408 241-9760 guest) Date: Wed, 22 Dec 1993 22:55:04 GMT Lines: 54 MYCONET currently "serves" three Internet mailing lists. To get the most recent list of lists, to request other information, or to suggest new mailing list topics, please send email to: postmaster@myconet.org MEDLIST This mailing list is concerned with discussing the medicinal uses of fungi. Mycologists (Professional and amateur) and medical researchers are encouraged to participate. Subscription address: medlist-request@myconet.org Posting address: medlist@myconet.org EDULIST This mailing list is concerned with educational applications of fungi, particularly as demonstrations in classroom projects. Educators (all levels) and parents are encouraged to participate. Subscription address: edulist-request@myconet.org Posting address: edulist@myconet.org DBLIST This mailing list discusses the development of mycological databases. Mycologists (professional and amateur) with experience or an interest in computer programming are encouraged to participate. This mailing list may also be useful to mycologists who are unfamiliar with computer technology but are interested in the potential (and constraints) of database development. Subscription address: dblist-request@myconet.org Posting address: dblist@myconet.org TO GET ON A LIST 1) Send a message addressed to the Subscription address. 2) Include the word "join" in text of the message. TO GET OFF A LIST 1) Send a message addressed to the Subscription address. 2) Include the word "remove" in text of the message. TO SEND A MESSAGE TO THE LIST 1) Send a message addressed to the Posting address 2) Do not use this address to get on or off a list!!!! TO CATCH UP ON PREVIOUS MESSAGES Each mailing list corresponds to a conference on MYCONET, which is available at no charge via direct dial-up at (408)866-9247. At this time telnet is not available. FTPmail is available but we have not figured out how to best set that up. If you have any suggestions, please contact: blaise.pabon@myconet.org ions, please contact: blaise.pabon@myconet.org