From owner-mycology@net.bio.net Tue Mar 01 22:00:00 1994
Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!europa.eng.gtefsd.com!news.umbc.edu!haven.umd.edu!purdue!mentor.cc.purdue.edu!inet.d48.lilly.com!mcvax4.d48.lilly.com!rm86670
Newsgroups: bionet.mycology
Subject: Re: Info on ISHAM ?
Message-ID: <1994Mar2.154320.1@mcvax4.d48.lilly.com>
From: rm86670@mcvax4.d48.lilly.com
Date: 2 Mar 94 15:43:20 EST
References: <CLnA6p.Asq@news.cis.umn.edu>
Distribution: world
Nntp-Posting-Host: mcvax4.d48.lilly.com
Lines: 27

In article <CLnA6p.Asq@news.cis.umn.edu>, herro001@maroon.tc.umn.edu (Michael J Herron-1) writes:
> I am looking for information on ISHAM.  I think it stads for the
> International Society of Human and Animal Mycology.  We have been working
> with the immunology of Candida infection and would like to more about this
> Society.
> 
> herro001@staff.tc.umn.edu
> Mike Herron
> 

Mike,

 ISHAM does mean Int'l Society of Human and Animal Mycology as you thought.
The outgoing General-Secretary is Michael G. Rinaldi, PhD, Dept. of
Pathology, Univ. of Texas Health Science Center, 7703 Floyd Curl Drive,
San Antonio, TX 78284-7750, USA. Telephone is (1)210-567-4132.

 I can't remember who the new Gen. Secretary is, but I'm sure that if
you write or call Mike Rinaldi, he can give you information regarding
ISHAM or direct you to the new Secretary.

 Another post referred to the ISHAM meeting being held this month in
Australia. Our next meeting will be in 1997 in Italy.
-- 
Michael A. Turik, MD               Preferred e-mail address:  miindy@aol.com

________________My opinions are my own (of course)____________________________

From owner-mycology@net.bio.net Thu Mar 03 22:00:00 1994
Path: biosci!IASTATE.EDU!cbronson
From: cbronson@IASTATE.EDU (Charlotte R Bronson)
Newsgroups: bionet.mycology
Subject: Stop Subscription
Date: 4 Mar 1994 18:51:08 -0000
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 3
Sender: daemon@net.bio.net
Distribution: bionet
Message-ID: <9403041851.AA29349@vincent1.iastate.edu>
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Please stop my subscription.
C. Bronson


From owner-mycology@net.bio.net Thu Mar 03 22:00:00 1994
Path: biosci!daresbury!not-for-mail
From: PAPAGEORGIOU GEORGIOS <gcpap@cyclades.nrcps.ariadne-t.gr>
Newsgroups: bionet.mycology
Subject: INVITATION TO GREEK BIOSCIENTISTS
Date: 4 Mar 1994 06:16:31 -0000
Lines: 45
Sender: daemon@mserv1.dl.ac.uk
Distribution: bionet
Message-ID: <2l6jnv$9nf@mserv1.dl.ac.uk>
X-Char-Esc: 29
X-Charset: ELOT_928
Original-To: mycology@dl.ac.uk

      * * * * INVITATION TO GREEK BIOLOGICAL SCIENTISTS * * * *

The Institute of Biology of the National Research Center Demokritos in 
Athens, Greece, is interested in compiling a list of Greek research 
scientists working in diverse fields of life sciences who might be 
interested in future staff openings at the Institute. The information 
will be used to formulate the expansion policy of the Institute.

Please send brief memo including name, addresses (postal mail, e-mail,
telefax, telephone), present affiliation and research area. A list of
publications will be helpful.

A rough sketch of research areas in the Institute of Biology includes,
but it is not limited to, the following:

BASIC BIOSCIENCE
Mammalian gene expression; cytokines and oncogenes; human serum growth
factors; cell regulation mechanisms; opioid peptides and receptors;
transmembrane signals; lectines; photosynthesis (function, regulation,
biogenesis); developmental theoretical biology.

APPLIED BIOSCIENCE
Environmental genotoxicity; radiation induced mutations; primary sea
productivity and phytoplanckton; chemical communication of insects
(feromones); management of insect populations; insect ecophysiology; 
insect nutrition; soil microbiology; uptake of radioactive nuclides by 
plants.
  
Please disseminate this information to other interested scientists you 
may know.

Thank you.     

Contact person:
Dr. George C. Papageorgiou, Director
NRC Demokritos, Institute of Biology
Athens, Greece 153 10
_______________________________
Telefax	: (301) 651 1767
Telephone	: (301) 652 2018
E-mail: gcpap@cyclades.nrcps.ariadne-t.gr
_______________________________




From owner-mycology@net.bio.net Sat Mar 05 22:00:00 1994
Path: biosci!bloom-beacon.mit.edu!gatech!swrinde!cs.utexas.edu!news.tamu.edu!128.194.15.2!leland
From: leland@straylight.tamu.edu (Leland Ellis)
Newsgroups: bionet.mycology
Subject: AGsDB, A Genus species DataBase
Date: 06 Mar 1994 03:25:54 GMT
Organization: W.M. Keck Center for Genome Informatics, Institute of Biosciences
	and Technology, Texas A&M University, Houston
Lines: 94
Distribution: world
Message-ID: <LELAND.94Mar5212554@straylight.tamu.edu>
NNTP-Posting-Host: straylight.tamu.edu


			      WWW-AGsDB

	      WWW-ACeDB Server for "A Genus species DataBase"

			   available on the

			      WWW Server

				of the

       W.M. Keck Center for Genome Informatics at the Institute
		      Biosciences and Technology
			 Texas A&M University
			       Houston

		  URL: http://keck.tamu.edu/ibt.html

WWW-AGsDB is a WWW interface to the data contained in AGsDB-1.0, which is
implemented in ACeDB-3.0, the latest (and still beta) version of Richard
Durbin and Jean Thierry-Mieg's ACeDB.  AGsDB is a prototype for a
multi-species database, with datatypes useful in the study of species
of agricultural and biomedical interest, as well as model organisms.
The interface utilizes the ACeDB Server software (nph-acedb3) developed
by Guy Decoux (decoux@moulon.inra.fr), and modified versions of the
models and tags of the human C21 database of Otto Ritter and co.  The
modifications provide for queries between interacting Loci, and between
species via Homologs.

Data from several species can be found in AGsDB:  filamentous fungi
(Aspergillus nidulans, Neurospora crassa), cows (with the corresponding
anchor loci for humans) and cotton (examples as demo).  Recently, we have
begun to enter data for the Cell Cycle, which brings in Homlogs between
Aspergillus and both budding (C. cerevisiae) and fission (S. pombe)
yeast.

The WWW-AGsDB server should be regarded as an experimental prototype.
We do find it useful, but the data models are still being experimented
with, and new data is always being added.  Finally, the db is nowhere
near comprehensive in the data included, but is illustrative of the
datatypes that we routinely use in the study of these organisms.

Futher development of both of the previously announced WWW-DataBases,
WWW-AAnDB (for Aspergillus nidulans) and WWW-ABtDB (for cows), is now
being continued in AGsDB (WWW-AAnDB and WWW-ABtDB have been phased out,
but the data therein is now in AGsDB).

The WWW-AGsDB server is accessible via the WWW Server of Keck-IBT.
This URL

http://keck.tamu.edu/ibt.html

will take you to the Keck-IBT HomePage -- clicking on the Keck-IBT
logo takes you to our 1st page of information, which has a link to
AGsDB.

Any feedback is very welcome at:

leland@straylight.tamu.edu

or

jkirk@keck.tamu.edu

AGsDB is the work of Leland Ellis, Jeff Kirk, Chris Floyd and Janis Bender
at Keck-IBT, with advice on Aspergillus from Tom Adams (Texas A&M) and Greg
May (Baylor College of Medicine), Neurospora from Dan Ebbole (Texas A&M),
cows from Jim Womack (Texas A&M), cotton from Russ Kohel (Texas A&M) and
the Cell Cycle from Greg May (Baylor College of Medicine).

----------------------------------------------------------------------------

Leland Ellis, Ph.D.
Professor of Biochemistry and Biophysics
Director, W.M. Keck Center for Genome Informatics
Institute of Biosciences and Technology
Texas A&M University
2121 Holcombe
Houston, Texas  77030
email: leland@straylight.tamu.edu
URL: http://keck.tamu.edu/ibt.html

----------------------------------------------------------------------------

--
Leland Ellis, Ph.D.
Professor of Biochemistry and Biophysics
Director, W.M. Keck Center for Genome Informatics
Institute of Biosciences and Technology
Texas A&M University
2121 Holcombe
Houston, Texas  77030
email: leland@straylight.tamu.edu
URL:   http://keck.tamu.edu/ibt.html

From owner-mycology@net.bio.net Mon Mar 07 22:00:00 1994
Newsgroups: bionet.mycology
Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!gatech!news-feed-2.peachnet.edu!umn.edu!news
From: brambl@graz.cbs.umn.edu (Robert Brambl)
Subject: Source of cyclosporin A
Message-ID: <CMD72t.72t@news.cis.umn.edu>
Sender: news@news.cis.umn.edu (Usenet News Administration)
Nntp-Posting-Host: graz.cbs.umn.edu
Reply-To: brambl@molbio.cbs.umn.edu (Robert Brambl)
Organization: University of Minnesota, Twin Cities
Date: Tue, 8 Mar 1994 21:16:41 GMT
Lines: 13

Hello, all:

We are attempting to find a source of cyclosporin A and/or FK506 for a  
study of mitochondrial membrane proteins in Neurospora.  ICN sold  
cyclosporin until recently, but they no longer have it available.  It  
would be helpful to receive either vendor information or the name of the  
manufacturer. 

Thanks and best wishes.

Robert Brambl
University of Minnesota
brambl@molbio.cbs.umn.edu

From owner-mycology@net.bio.net Mon Mar 07 22:00:00 1994
Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!europa.eng.gtefsd.com!news.umbc.edu!eff!news.kei.com!yeshua.marcam.com!zip.eecs.umich.edu!umn.edu!msus1.msus.edu!vax1.mankato.msus.edu!vengeance
Newsgroups: bionet.mycology
Subject: Omaha Project
Message-ID: <1994Mar8.085124.2377@vax1.mankato.msus.edu>
From: vengeance@vax1.mankato.msus.edu
Date: 8 Mar 94 08:51:24 -0500
Organization: Mankato State University
Lines: 9

   I am trying to build a list of names and E-Mail addresses of
people in the Omaha Nebraska area for a school related project.
   If you live in Omaha or go to school there or know someone
that does and will be around for three months or more, please
reply via E-Mail to Vengeance@vax1.mankato.msus.edu.

Thank you very much!

Ryan Krueger

From owner-mycology@net.bio.net Mon Mar 07 22:00:00 1994
Path: biosci!CPHKVX.CPHK.HK!93809626
From: 93809626@CPHKVX.CPHK.HK (LAM FUNG)
Newsgroups: bionet.mycology
Subject: Ask for software
Date: 8 Mar 1994 05:16:16 -0000
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 10
Sender: daemon@net.bio.net
Distribution: bionet
Message-ID: <01H9QBDZZAQC8WXQI1@cphkvx.cphk.hk>
Reply-To: 93809626@cphkvx.cphk.hk
NNTP-Posting-Host: net.bio.net

Dear Netters,
            May I know more information about "Zeiss ZIDAS Image Analysis
System"? It is because I want to measure the diameter / area of the fungal
growth. Is there any other system to do this task? May I have all those
information? Please help me!!!!
            Thanks in advance.

Paul Lam Fung
City Polytechnic of Hong Kong
e-mail address: 93809626@cphkvx.cphk.hk

From owner-mycology@net.bio.net Tue Mar 08 22:00:00 1994
Path: biosci!MOOSE.UVM.EDU!lvaillan
From: lvaillan@MOOSE.UVM.EDU (Lisa Vaillancourt)
Newsgroups: bionet.mycology
Subject: more novozyme problems
Date: 9 Mar 1994 20:27:59 -0000
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 27
Sender: daemon@net.bio.net
Distribution: bionet
Message-ID: <9403092028.AA89666@moose.uvm.edu>
NNTP-Posting-Host: net.bio.net

I hope somebody out there can help, lately we have been having a very hard
time making transformable protoplasts of Schizophyllum commune using our
normal novozyme protocol.  We recently purchased novozyme 234 batch PPM
4356 from Interspex.  It worked OK for one of our strains when used at a
very low concentration for a shorter period of time than normal, but since
then we have not had any luck.  It seems that if we use it at a
concentation high enough to get protoplasts (between 5 and 10 mgs/ml final
concentration) the protoplasts do not transform.  However, if we use less
(between 0.5 and 5 mgs/ml) the digestion is really poor and we get few or
no protoplasts.  Our protocol calls for digestion of chopped mycelium in
buffered 1M MgCl2 at 30C for about 5 or 6 hours, followed by mixing with an
equal volume of water, thus making the concentration 0.5M MgCl2, followed
by spinning relatively slowly to pellet the larger pieces of mycelial
debris, followed by adding an equal volume of 1M sorbitol and spinning
harder to bring down the protoplasts.  When we look at the mycelium after
digestion with the lower concentrations of novozyme, it still looks mostly
intact and there are few free protoplasts, but there are many visible still
trapped in what's left of the hyphae.  If we use higher concentrations, so
that there are more protoplasts, they transform very poorly, though they
look fine and usually regenerate well.  HELP!!  We have been told that this
novozyme does better at 37C than at 30, and that it doesn't work well in
magnesium..and what is the opinion of the usefulness of including beta
mercaptoethanol in the digestion mix??  We are planning to do a
troubleshooting experiment, can anyone give us other ideas to try??  We
would really appreciate it.  



From owner-mycology@net.bio.net Wed Mar 09 22:00:00 1994
Path: biosci!VIOLET.BERKELEY.EDU!jtaylor
From: jtaylor@VIOLET.BERKELEY.EDU (john taylor)
Newsgroups: bionet.mycology
Subject: Fungal systematist position at U.C., Berkeley
Date: 10 Mar 1994 01:06:45 -0000
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 40
Sender: daemon@net.bio.net
Distribution: bionet
Message-ID: <199403100107.RAA22746@violet.berkeley.edu>
NNTP-Posting-Host: net.bio.net

Dear Mycology net:

        The UC Berkeley Herbarium and the Department of Plant Biology are
searching for a systematic mycologist.  The closing date for applications
is April 15, 1994.  


UNIVERSITY OF CALIFORNIA AT BERKELEY
FUNGI CURATOR, UNIVERSITY HERBARIUM

        The University of California at Berkeley seeks an outstanding
systematic mycologist with responsibilities as curator of fungi and lichens
(Assistant Specialist, 11 months appointment) in the University Herbarium
and as an Adjunct Professor in the Department of Plant Biology starting on
or after July 1, 1994.  Applications are invited from individuals whose
research interests are in systematics, ecology, and evolutinary biology of
free-living and lichenized fungi.  The successful candidate will be
expected to provide public service in fungal identification, curate and
enhance the Herbarium fungal collection, develop an independent research
program, and participate in undergraduate and gradulate level instruction
in the Department.  Applicants must have a Ph.D.  Starting salary will be
approximately $35,000.  
        A full CV, a statement of current research and future interests, a
statement of curatorial and teaching experience, and three letters of
reference, should be sent by April 15, 1994 to: Chair, Search Committee,
Department of Plant Biology, 111 Koshland Hall, University of California,
Berkeley, CA 94720.  
The University of California is an Equal Opportunity/Affirmative Action
Employer.

____________________________________
From:   John Taylor
        Department of Plant Biology
        111 Koshland Hall
        University of California
        Berkeley, CA  94720
        Tel (510) 642-5366
        Fax (510) 642-4995
        internet jtaylor@violet.berkeley.edu


From owner-mycology@net.bio.net Wed Mar 09 22:00:00 1994
Path: biosci!daresbury!trane.uninett.no!sunic!EU.net!howland.reston.ans.net!wupost!bcm!usenet
From: gsmay@bcm.tmc.edu (Gregory May)
Newsgroups: bionet.mycology
Subject: Re: more novozyme problems
Date: 10 Mar 1994 17:59:39 GMT
Organization: Baylor College of Medicine
Lines: 8
Message-ID: <2lnn6b$ojf@gazette.bcm.tmc.edu>
References: <9403092028.AA89666@moose.uvm.edu>
NNTP-Posting-Host: ai.cellb.bcm.tmc.edu
X-Newsreader: WinVN version 0.82


Over the years variation in batches of novozyme has presented some
 real problems.  Some people have found that passing it over a sephadex 
G-25 column helps.  We have used precipitation from ammonium sulfate
for some batches.  See Osmani et al., 1987 JCB 104, 1495.  

I hope this helps some.
Greg

From owner-mycology@net.bio.net Wed Mar 09 22:00:00 1994
Path: biosci!exec.niaid.pc.niaid.nih.gov!Dennis_M._Dixon
From: Dennis_M._Dixon@exec.niaid.pc.niaid.nih.gov ("Dennis M. Dixon")
Newsgroups: bionet.mycology
Subject: NIH Mycology Workshop/Meeting
Date: 10 Mar 1994 21:32:03 -0000
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 122
Sender: daemon@net.bio.net
Distribution: bionet
Message-ID: <2d7f9386@exec.niaid.pc.niaid.nih.gov>
NNTP-Posting-Host: net.bio.net

Dear Network Members,

Here is an announcement of a meeting that some of you might be 
interested in attending.  If so, I hope to see you there.  If you have 
questions about the meeting or registration, please make your initial 
inquiries to the University of Arizona Office of Continuing Education 
at the numbers listed below (under Registration Information).
                                   ------------------------
Best wishes, and please read the message below my address.

Dennis M. Dixon
Bacteriology and Mycology Branch
National Institute of Allergy and Infectious Diseases
National Institutes of Health
Bethesda, MD 20892
Telephone:  301-496-7728
FAX:        301-402-2508
Netmail:    dmd@exec.niaid.pc.niaid.nih.gov 
========================================================================
NIAID Workshop Series in Medical Mycology --- The Second in the Series

Molecular and Immunologic Approaches to the Diagnosis and Treatment of 
                        Systemic Mycoses

Northern Arizona University; Flagstaff, Arizona; 8-11 June 1994

The National Institute of Allergy and Infectious Diseases is sponsoring a
workshop focusing on fundamental investigation as it relates to the 
diagnosis and treatment of fungal infections.  The NIAID recognizes the 
growing importance of medical mycology and opportunities provided by 
recent advances in molecular biology and immunology.  This workshop will 
offer examples of how new tools may enable improvements in the 
recognition and management of patients.  The workshop is co-chaired by 
John Galgiani and Michael Pfaller.

Format
------
There will be two sessions each day.  Session topics have been chosen as
examples of original approaches rather than as comprehensive review. 
Participant interaction will be facilitated by discussions in small working
groups on each of the first two days.  Workshop attendees may offer topics 
of their own to expand the program.  These would be shown as poster
presentations, or in a few selected cases, as oral presentations in one of 
the sessions listed below.  

Costs
-----
Dormitory rooms have been reserved on the Northern Arizona University 
campus. Limited travel support should be available.  In order to be 
considered for support, eligibility for NIH National Research Service 
Awards for Individual Research Fellows (F32) is required.

Registration fee:       $75 (nonrefundable)
Room and meals:         $132 per person (3 days, 3 nights)
                        $176 per person (3 days, 4 nights)

Registration Information
------------------------
Enrollment will be limited.  
Registration deadline: May 10, 1994.
For registration information contact:

            Office of Continuing Medical Education
            Arizona Health Sciences Center
            Tucson, Arizona 85724
            (Phone)     602-626-7832  or, 1-800-328-5868
            (Fax)       602-626-2427

Workshop Schedule
-----------------

Molecular and Immunologic Approaches to the Diagnosis and Treatment of 
                        Systemic Mycoses

Northern Arizona University; Flagstaff, Arizona; 8-11 June 1994

                        WEDNESDAY, JUNE 8       

Arrival and Registration; Mixer

                        THURSDAY, JUNE 9  

Opening Lecture: 
Status and Limitation of Diagnosis and Therapy of Fungal Infections
      William Dismukes

Scientific Sessions:
Targets for Inhibiting Proliferation or Virulence
      William Timberlake - Moderator
            Christophe D'Enfert
            William Fonzi

Diagnosis by Detection of Metabolic Products, Antigens, or Antibodies
      Brian Wong - Moderator
            Tom Kozel
            Bruce Kline

                        FRIDAY, JUNE 10

Molecular Approaches to New Diagnostic Test Development
      Russell Enns - Moderator
            John Taylor
            Mahmoud Ghannoum
            Ted White
            John Galgiani

Biologic Response Modifiers for Therapy
      John Edwards - Moderator
            Stu Levitz
            Richard Locksley

                        SATURDAY, JUNE 11

Drug Discovery
      Richard Hector - Moderator
            Paul Lartey
            Myra Kurtz

Panel Discussion
      Private, Public, and Academic Partnerships



From owner-mycology@net.bio.net Thu Mar 10 22:00:00 1994
Newsgroups: bionet.mycology
Path: biosci!daresbury!keele!uknet!pipex!howland.reston.ans.net!wupost!gumby!newsxfer.itd.umich.edu!nntp.cs.ubc.ca!alberta!quartz.ucs.ualberta.ca!kakwa.ucs.ualberta.ca!NoFC.Forestry.CA!medusa!davidip
From: davidip@mdo.nofc.forestry.ca (David Ip)
Subject: Request for Myconet Listserv address
Message-ID: <CMCs2B.9MH@NoFC.Forestry.CA>
Keywords: myconet subscription request
Sender: usenet@NoFC.Forestry.CA (Usenet)
Nntp-Posting-Host: medusa.mdo.nofc.forestry.ca
Reply-To: davidip@mdo.nofc.forestry.ca
Organization: MDO, Northern Forestry Center, Northwest Region, Forestry Canada
Date: Tue, 8 Mar 1994 15:51:46 GMT
Lines: 8

Would someone kindly post or send me the Listserv address for subscribing to Myconet, the existence of which I have just learned.

TIA.

David Ip
Canadian Forest Service
Winnipeg, Manitoba Canada
davidip@mdo.nofc.forestry.ca

From owner-mycology@net.bio.net Sun Mar 13 22:00:00 1994
Path: biosci!agate!howland.reston.ans.net!wupost!bcm!usenet
From: gsmay@bcm.tmc.edu (Gregory May)
Newsgroups: bionet.mycology
Subject: Request for email address.
Date: 14 Mar 1994 18:42:06 GMT
Organization: Baylor College of Medicine
Lines: 8
Message-ID: <2m2b5u$bf1@gazette.bcm.tmc.edu>
NNTP-Posting-Host: ai.cellb.bcm.tmc.edu
X-Newsreader: WinVN version 0.82

I am in need of an email address for Dr. Pierre de Wit at the
Wageningen Agricultural University, Wageningen, The Netherlands.
If anyone knows this information I would be greatful if you could email
it to me.

Thanks in advance,
Gregory May
gsmay@bcm.tmc.edu

From owner-mycology@net.bio.net Tue Mar 15 22:00:00 1994
Path: biosci!VENUS.TAMU.EDU!bam1587
From: bam1587@VENUS.TAMU.EDU (Bruce McDonald)
Newsgroups: bionet.mycology
Subject: Graduate Student Assistantships
Date: 16 Mar 1994 15:13:04 -0000
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 32
Sender: daemon@net.bio.net
Distribution: bionet
Message-ID: <199403161513.HAA17007@net.bio.net>
NNTP-Posting-Host: net.bio.net

GRADUATE ASSISTANTSHIPS AVAILABLE THROUGH THE PROGRAM FOR BIOLOGY OF
FILAMENTOUS FUNGI AT TEXAS A&M UNIVERSITY

The Program for Biology of Filamentous Fungi has five graduate student
stipends available each year for qualified US citizens through a NSF
training grant.  The purpose of this grant is to provide first-year support
to students interested in any aspect of fungal biology.  Student support
for the first year includes $14,000 for a stipend and additional support to
cover full tuition costs and medical benefits.  

Students may receive the Ph.D. degree in Plant Pathology, Biology or
Genetics.  As part of the Program for Biology of Filamentous Fungi at Texas
A&M University, students will be associated with one of the largest groups
of fungal biologists at a North American university.  Literature on the
Program will be sent upon request.

Assistantships are available for the Fall, 1994 semester.  The Program will
be glad to send additional information to interested students and faculty
advisors.  Please contact the Program via Internet (preferred), letter,
phone, or FAX.  

Program for Biology of Filamentous Fungi
c/o Bruce McDonald
Dept. Plant Pathology and Microbiology
Texas A&M University
College Station, TX  77843-2132
USA
Internet:  BAM1587@ZEUS.TAMU.EDU
Bitnet:  BAM1587@TAMZEUS
Phone:  409-845-7311
FAX:  409-845-6483


From owner-mycology@net.bio.net Tue Mar 15 22:00:00 1994
Path: biosci!bcm!usenet
From: gsmay@bcm.tmc.edu (Gregory May)
Newsgroups: bionet.mycology
Subject: Thanks to all.
Date: 16 Mar 1994 20:05:05 GMT
Organization: Baylor College of Medicine
Lines: 5
Message-ID: <2m7oph$la0@gazette.bcm.tmc.edu>
NNTP-Posting-Host: ai.cellb.bcm.tmc.edu
X-Newsreader: WinVN version 0.82

Thanks to everyone that provided me with information to Dr. de Wit's email
address.  I now have it.

Gregory May
gsmay@bcm.tmc.edu

From owner-mycology@net.bio.net Thu Mar 17 22:00:00 1994
Newsgroups: bionet.mycology
Path: biosci!bcm!cs.utexas.edu!uunet!mnemosyne.cs.du.edu!nyx10!anon015b
From: anon015b@nyx10.cs.du.edu (Name withheld by request)
Subject: Expert wanted
Message-ID: <1994Mar18.010937.19170@mnemosyne.cs.du.edu>
X-Disclaimer: Nyx is a public access Unix system run by the University
 	of Denver for the Denver community.  The University has neither
 	control over nor responsibility for the opinions of users.
Sender: usenet@mnemosyne.cs.du.edu (netnews admin account)
Organization: Nyx, Public Access Unix at U. of Denver Math/CS dept.
Distribution: usa
Date: Fri, 18 Mar 94 01:09:37 GMT
Lines: 6


STATE OF ALASKA needs a mold expert.  May involve travel/testimony.
Contact- J. Ron Sutcliffe , Assistant Attorney General
JSJRS1@acad1.alaska.edu



From owner-mycology@net.bio.net Thu Mar 17 22:00:00 1994
Path: biosci!daresbury!not-for-mail
From: SCHURENF <SCHURENF@biol.rug.nl>
Newsgroups: bionet.mycology
Subject: help
Date: 18 Mar 1994 10:12:08 -0000
Organization: Department of Biology, RUGroningen
Lines: 1
Sender: daemon@mserv1.dl.ac.uk
Distribution: bionet
Message-ID: <2mbupo$hfu@mserv1.dl.ac.uk>
Original-To: mycology@dl.ac.uk

How can I read messages from the mycology mail newsgroup?

From owner-mycology@net.bio.net Thu Mar 17 22:00:00 1994
Path: biosci!VM42.CSO.UIUC.EDU!FGSC%UKANVM.bitnet
From: FGSC%UKANVM.bitnet@VM42.CSO.UIUC.EDU (Craig Wilson)
Newsgroups: bionet.mycology
Subject: FGSC Catalogue on line
Date: 18 Mar 1994 15:10:34 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 36
Sender: daemon@net.bio.net
Distribution: bionet
Message-ID: <199403182310.PAA16555@net.bio.net>
NNTP-Posting-Host: net.bio.net

To Fungal Genetics People:
    There is now an on-line version of the Fungal Genetics Stock Center
catalogue at a WWW server here at the University of Kansas.  I do not yet
have information on all of our stocks loaded in yet, but that should be done
soon and in the meantime all single mutants, plasmids, gene libraries, wild
type strains, and chromosome aberrations are listed.  I hope many of you will
take a look at the information, look for mistakes, and report back to me on
what does and does not work.  In future, we should be able to put things such
as the Fungal Genetics Newsletter, abstracts from Fungal Genetics Meetings,
etc. on this server.
   The URL for direct access is:
http://ukanaix.cc.ukans.edu/cwis/units/fgsc/main.html

As alternatives, you can get to this via the WWW list of hosts, arranged by
country.  Skip down to Kansas, where there is a listing for University of
Kansas KUfacts, Campus Wide Information Server, and another for the TISL lab
at KU, which also contains a link to KUfacts.

However you get to KUfacts, select 'Departmental Information' on the opening
page, 'Medical Center Resource' on the next page, and 'Fungal Genetics Stock
Center' on the third page.

Another possible way to get to KUfacts is via a Gopher server maintained by t
TISL.  This Gopher is on the Univ of Minnesota list.

Let me know if I have made mistakes in listing these paths, or if the FGSC
data is not presented in a clear manner.

Thanks for your help.

Craig Wilson
FGSC
Dept of Microbiology
Univ of kansas Medical Center
Kansas City KS 66160-7420 USA
fgsc@ukanvm.cc.ukans.edu

From owner-mycology@net.bio.net Sun Mar 20 22:00:00 1994
Path: biosci!MIRANDA.UMDS.AC.UK!fsymons
From: fsymons@MIRANDA.UMDS.AC.UK (Fernley Symons)
Newsgroups: bionet.mycology
Subject: (none)
Date: 21 Mar 1994 11:52:30 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 1
Sender: daemon@net.bio.net
Distribution: bionet
Message-ID: <1896.9403211941@miranda.umds.ac.uk>
NNTP-Posting-Host: net.bio.net

unsubscribe

From owner-mycology@net.bio.net Sun Mar 20 22:00:00 1994
Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!newsrelay.iastate.edu!apollo1.cacd.cr.rockwell.com!news-feed-1.peachnet.edu!concert!bigblue.oit.unc.edu!sunSITE!london
From: london@sunSITE.unc.edu (Larry London)
Newsgroups: bionet.mycology,bionet.general,sci.bio,sci.agriculture
Subject: Compost spores and health.
Date: 21 Mar 1994 20:18:10 GMT
Organization: University of North Carolina, Chapel Hill
Lines: 32
Message-ID: <2mkve2$khr@bigblue.oit.unc.edu>
NNTP-Posting-Host: calzone.oit.unc.edu
Summary: Reply to: m1dorrin@attmail.com or sanet-mg@twosocks.ces.ncsu.edu
Xref: biosci bionet.mycology:383 bionet.general:8295 sci.bio:7623

From m1dorrin@attmail.com Mon Mar 21 14:58:13 1994
Date: 21 Mar 94 18:06:20 GMT
From: Mike Dorrington <m1dorrin@attmail.com>
To: sanet-mg@ces.ncsu.edu
Subject: compost spores & health

Can anyone answer below (forwarded) message?

>We are looking for information on the possible health
>effects of a mold spore-- Aspergillus fumigatus-- that is
>associated with composting operations and can also be found
>in the air and in buildings.  A study by the New York state
>Dept. of Health came out last week with information
>suggesting that it was not the cause of respiratory or other
>health problems around an Islip Long Island composting
>facility.  At the same time the study said composting
>facilities should not be located near hospitals, in
>particular, medical facilities with bone marrow transplant
>operations.  If you have any information on this subject,
>please send me an e-mail message.  Thanks!  -- David Hess,
>Executive Director, Pennsylvania Senate Environmental
>Resources & Energy Committee.











From owner-mycology@net.bio.net Mon Mar 21 22:00:00 1994
Path: biosci!bcm!cs.utexas.edu!news.tamu.edu!128.194.15.2!leland
From: leland@straylight.tamu.edu (Leland Ellis)
Newsgroups: bionet.mycology
Subject: WWW-FGSC
Date: 22 Mar 1994 17:39:26 GMT
Organization: W.M. Keck Center for Genome Informatics, Institute of Biosciences
	and Technology, Texas A&M University, Houston
Lines: 27
Distribution: world
Message-ID: <LELAND.94Mar22113926@straylight.tamu.edu>
NNTP-Posting-Host: straylight.tamu.edu

Three cheers for the WWW rendition of the FGSC catalog.  However, I found it only
after investigating via CERN where in Kansas it actually resides -- this URL
works:

http://kufacts.cc.ukans.edu/cwis/units/fgsc/main.html

It is somewhat slow, especially as for each catagory it retrieves all entries, e.g.,
all of the mailing list, or all strains.  This can be alleviated by breaking these
large flat html files into small chunks, accessed from a menu page.

Thanks Craig.  Having a ready source of this info, and the ability w/ Mosaic
to download ascii data, is a great help (we have done quite a bit from the
FGSC catalog by scanning).

Cheers,

Leland
--
Leland Ellis, Ph.D.
Professor of Biochemistry and Biophysics
Director, W.M. Keck Center for Genome Informatics
Institute of Biosciences and Technology
Texas A&M University
2121 Holcombe
Houston, Texas  77030
email: leland@straylight.tamu.edu
URL:   http://keck.tamu.edu/ibt.html

From owner-mycology@net.bio.net Mon Mar 21 22:00:00 1994
Path: biosci!agate!darkstar.UCSC.EDU!arapaho!nathan
From: nathan@cse.ucsc.edu (Nathan J. Wilson)
Newsgroups: bionet.mycology
Subject: Agaricus bisporus/brunnescens found
Date: 22 Mar 1994 23:16:28 GMT
Organization: UC Santa Cruz CIS/CE
Lines: 13
Message-ID: <2mnu8c$59j@darkstar.UCSC.EDU>
NNTP-Posting-Host: arapaho.cse.ucsc.edu
X-Newsreader: TIN [version 1.2 PL2]

A little while ago there were some notes about wild collections of
the cultivated Agaricus species.  A friend of mine has some 'wild'
specimens coming up in his yard, so if the folks that were interested
are still interested, please get in touch ASAP.  You can reach me
by phone at (408)423-3773.
   -------------------------    _________
         Nathan Wilson         <_________>
      nathan@cse.ucsc.edu         _|_|_       It is no dream!
      Co-Science Advisor          \___/    Matsutake are growing
   Minister of Local Forays        | |        On the belly of the mountain.
Fungus Federation of Santa Cruz    \_/ *83--                -Shigetaka



From owner-mycology@net.bio.net Mon Mar 21 22:00:00 1994
Path: biosci!bcm!cs.utexas.edu!math.ohio-state.edu!magnus.acs.ohio-state.edu!bdemars
From: bdemars@magnus.acs.ohio-state.edu (Brent G DeMars)
Newsgroups: bionet.mycology,bionet.plants,sci.bio.ecology,sci.bio
Subject: Antarctic fungus - looking for ID help & collaboration
Date: 22 Mar 1994 22:29:27 GMT
Organization: The Ohio State University
Lines: 13
Message-ID: <2mnrg7$pg3@charm.magnus.acs.ohio-state.edu>
NNTP-Posting-Host: beauty.magnus.acs.ohio-state.edu
Xref: biosci bionet.mycology:385 bionet.plants:2870 sci.bio.ecology:2405 sci.bio:7646


I recently obtained about 100 soil samples from Palmer Peninsula.  I'm 
primarily interested in culturing root fungi; however, I have been 'culturing' 
other types also.

I am no mycologist and am looking for someone skilled in identifying fungi via 
fruiting bodies.  As of date about 15 different types of fruiting bodies have 
developed in my cultures.  I'd like to ID them and determine if there is 
anything new from these samples.

If you're interested in seeing what I have, please write.

Brent

From owner-mycology@net.bio.net Tue Mar 22 22:00:00 1994
Newsgroups: bionet.mycology
Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!torn!news.unb.ca!csd.unb.ca!UNBSJ.CA!c0hx
From: c0hx@UNBSJ.CA (JASON K. DOBRANIC)
Subject: Scale used to evaluate interference competition
Message-ID: <c0hx.29.0@UNBSJ.CA>
Sender: news@jupiter.sun.csd.unb.ca
Organization: UNB Saint John Campus
Date: Wed, 23 Mar 1994 00:54:49 GMT
Lines: 34

	I was wondering if any of you working on interference competition 
bioassays have a good scale for evaluating reaction types. I am so far 
aware of the following one using the index of antagonism:


Points				Reaction type 

A		Mutual intermingling of the two organisms
0

B		Mutual inhibition on contact; the space between the colonies 
1		is small but clearly marked

C		Mutual inhibition at a distance
2

D		Inhibition of one organism on contact; the antagonist 
3		continues to grow, unchanged or at a reduced rate, through 
		the colony of the inhibited organism.

E		Inhibition of one organism at a distance; the antagonist 
4		continues to grow through the resulting clear zone at an 
		unchanged or reduced rate.

Index of Antagonism:

IA = [n(A*0) + n(B*1) + n(C*2) + n(D*3) + n(E*4)] / the number of trials

where n is the frequency of the reaction type.
(After Wicklow et al, 1980; Strongman, 1986)

Is there in your opinion a better way of evaluating this? Also for slow 
growing antagonists it seems difficult to choose between C and E.
Thanks for any help!

From owner-mycology@net.bio.net Tue Mar 22 22:00:00 1994
Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!moe.ksu.ksu.edu!kuhub.cc.ukans.edu!elspeth
From: elspeth@kuhub.cc.ukans.edu
Newsgroups: bionet.mycology
Subject: Re: morel mushrooms
Message-ID: <1994Mar23.152133.60858@kuhub.cc.ukans.edu>
Date: 23 Mar 94 15:21:33 CST
References: <Cn30AA.AuJ@lehman.com> <2modii$b7a@news.u.washington.edu>
Organization: University of Kansas Academic Computing Services
Lines: 25

In article <2modii$b7a@news.u.washington.edu>, kcarroll@u.washington.edu (Kris Carroll) writes:
> In article <Cn30AA.AuJ@lehman.com> jdewitt@lehman.com (Joel DeWitt)  
> writes:
>> Is it possible to culture Morels, or all those Morels out there picked  
> in the wild?
>> Thanks in advance for your woodland intelligence on this.
> 
> Fungi Perfecti, PO Box 7634, Olympia WA 98507
> sells giant morel spores, books and kits.
> Catalogue says inoculations should occur between May and Jan for crops the  
> following Spring.
> see stump removal thread for phone #s
> Kris Carroll
This thread came up on rec.gardens.  Anybody here know if it's
possible to grow morels (and get them to fruit)?  Is it a good idea to
buy spores (of this or other species)?  Anybody heard of the company?  
Crosspost any comments to rec.gardens, please.
-- 
-----------------------------------------------------------------------
internet: elspeth@kuhub.cc.ukans.edu  *    SnailNet: Elizabeth B. Naime
bitnet: elspeth@ukanvm.bitnet         *               German Department
                                      *            University of Kansas
  * Standard Disclaimers Apply *      *        Lawrence, KS  66045-2127
-----------------------------------------------------------------------
----         Open mouth. Insert foot. Echo internationally.        ----

From owner-mycology@net.bio.net Tue Mar 22 22:00:00 1994
Path: biosci!BULL.CC.USM.EDU!GLEN_SHEARER
From: GLEN_SHEARER@BULL.CC.USM.EDU (GLEN SHEARER)
Newsgroups: bionet.mycology
Subject: G6PDH
Date: 23 Mar 1994 09:24:14 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 10
Sender: daemon@net.bio.net
Distribution: bionet
Message-ID: <940323.11242060.035379@USM.CP6>
NNTP-Posting-Host: net.bio.net

I've heard that Neurospora mutants with a lesion in
glucose-6-phosphate dehydrogenase have altered morphology and
are more "yeast like".  Also interesting is that G6PDH mutants
of Saccharomyces are deficient in sulfite reductase.
 
Anyone know more about this?
 
Glen S.
 
<glen_shearer@bull.cc.usm.edu>

From owner-mycology@net.bio.net Tue Mar 22 22:00:00 1994
Path: biosci!CMGM.STANFORD.EDU!springer
From: springer@CMGM.STANFORD.EDU (Matt Springer)
Newsgroups: bionet.mycology
Subject: Neurospora G6PDH
Date: 23 Mar 1994 15:57:27 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 14
Sender: daemon@net.bio.net
Distribution: bionet
Message-ID: <199403232357.PAA08623@cmgm.stanford.edu>
NNTP-Posting-Host: net.bio.net

With regards to Glen Shearer's inquiry about glucose-6-phosphate dehydrogenase
mutants, the N. crassa mutants frost (fr), balloon (bal), and colonial-2 
(col-2), none of which are necessarily G6PDH lesions, are all nonetheless
defficient in G6PDH.  To the naked eye, these mutants might appear to be more
"yeast-like" in that they grow in dinstinct colonies, rather than exhibiting
the usual spreading growth.  However, on the microscopic level, the fr mutant
does not conidiate by budding, favoring a twisted, gnarled branching that
makes it resemble coral when observed by SEM.  On this level, then, it is
even LESS yeast-like than the wild-type.  I don't know what the other two
mutants look like at the microscopic level.

-Matt Springer

(springer@cmgm.stanford.edu)

From owner-mycology@net.bio.net Wed Mar 23 22:00:00 1994
Newsgroups: bionet.mycology
Path: biosci!agate!howland.reston.ans.net!pipex!sunic!trane.uninett.no!eunet.no!EU.net!ub4b!info-sparc1.info.ucl.ac.be!NewsWatcher!user
From: Moulliard@mbla.ucl.ac.be (Moulliard)
Subject: MUCL Centenary 1894-1994
Message-ID: <Moulliard-240394122327@130.104.82.1>
Followup-To: bionet.mycology
Sender: news@info.ucl.ac.be (News Administrator)
Nntp-Posting-Host: 130.104.82.1
Organization: MUCL
Date: Thu, 24 Mar 1994 12:23:27 +0100
Lines: 219

*************************************************

				JUNE 29, 1994
			LOUVAIN-LA-NEUVE, BELGIUM


				1894-1994

	MYCOTHEQUE DE L'UNIVERSITE CATHOLIQUE DE LOUVAIN
				MUCL CENTENARY

		"FUNGAL TAXONOMY AND TROPICAL MYCOLOGY: 
				QUO VADIS  ?"

*************************************************

INVITATION

Professor P. Macq, Rector of the UniversitŽ Catholique de Louvain, the UCL
Mycothque, the UCL Laboratory of Systematic and Applied Mycology and the
Coordinator of the Belgian Coordinated Collections of Microorganisms (BCCM)
have the pleasure of inviting you to celebrate, the

		100th Anniversary of MUCL

		on Wednesday, June 29th, 1994.

On this occasion, an international workshop will be held on 

"FUNGAL TAXONOMY AND TROPICAL MYCOLOGY: QUO VADIS ?"

Modern taxonomy, including mycotaxonomy, relies in an increasing degree on
new molecular approaches. 

As such, one can observe that the traditional morphotaxonomic approach is
more and more complemented - or sometimes even substituted - by
chemotaxonomic methods or genome-based analyses. Questioning the (added)
value and mutual complementarity of all available tools - both classical
and modern - is essential to be able to handle the systematic
classification problem in an integrated and effective way.

On the other hand, the greater part of the fungal biodiversity has still to
be discovered and described especially in the tropical regions. As these
are mainly situated in developing countries, one has to discuss - in the
spirit of the Rio de Janeiro earth summit - key topics such as training,
technology transfer and the set-up of local 'ex situ' collections. 

During such an exercise, special attention has to be given to taxonomic and
preservation methods that are not only effective but also widely
applicable, user-friendly and robust.

*************************************************

				PROGRAMME

				June 29th, 1994

			1894-1994 MUCL Centenary

9:00 to 12:00 am: Centenary celebration

	H. Naveau, UCL
		Opening lecture.
	P. Macq, Rector, UCL
	G.L. Hennebert, UCL
		Mycothque de l'UniversitŽ Catholique de Louvain (MUCL): 
		100 Years of existence and activity of the fungus culture 
		collection.

Guest speakers: 
	D. Hawksworth, IMI, U.K.
		Fungal genetic resource collections and biodiversity.
	D. van der Mei, CBS, MINE, Netherlands
		The fungus culture collections in Europe.
	J. De Brabandere, BCCM, Belgium
		The Belgian Coordinated Collections of Microorganisms.

12:00 to 1:30 pm: Buffet of Belgian specialties

1:30 to 6:00 pm: Workshop (provisional titles)

Introduction: 
	G.L. Hennebert, UCL
		Fungal taxonomy and tropical mycology: Quo vadis ?

	Guest speakers: 
	C.P. Kurtzman, NRRL, USA
		Molecular taxonomy in the yeast fungi: present and future.
	M. Blackwell, Louisiana State University, USA
		Phylogeny of filamentous fungi deduced from analysis of 
		molecular characters: present and future.
	J. Rammeloo, National Botanical Garden, Belgium
		Importance of morphological and anatomical characters in 
		fungal taxonomy.
	M.F. Roquebert, Natural History Museum, France
		Possible progress of modern morphological analysis in fungal 
		taxonomy.
	A.J. Masuka, Forest Research Center, Zimbabwe
		Mycofloristic studies in South Central Africa: status, 		constraints,
opportunities and strategies.
	A. Peerally, University of Mauritius, Mauritius
		Strategies for the development of tropical mycology.
	E.J. Da Silva, MIRCEN, UNESCO, France
		The role of the Microbiological Resources Centers (MIRCEN) 
		in the development of mycology in the tropics.

Discussion and conclusions.

Evening: Anniversary Banquet 

June 30th & July 1st, 1994

	ECCO XIII & MINE: annual meetings
	only for ECCO and MINE members.

*************************************************
CALL FOR POSTERS

Topics concerning the workshop are welcome. Authors are kindly requested 
to send the title and an abstract of their poster before May 1st, 1994.

*************************************************
FEES

Registration for Centenary celebration and workshop are without charge. 
Booking for the accommodation and banquet has to be paid. 
The fee for the Anniversary banquet is 1.800 BEF (US$ 50). 

*************************************************
TRANSPORTATION

By air

All airlines provide air service to the International Brussels Airport in 
Zaventem, about 30 km from Louvain-la-Neuve. A shuttle between the 
Airport and Louvain-la-Neuve will be organized on Tuesday, June 28th, 
1994 for participants.

By train

Inter-City trains operate every 30 min. from Brussels North, Central and 
Midi Stations to Ottignies (direction Namur). At Ottignies, there is a
train to 
Louvain-la-Neuve. 

By road

Participants arriving by road should take the E-411 motorway  Bruxelles-
Namur-Luxembourg and exit at n¡ 7 (coming from Brussels) or exit at n¡ 9 
(coming from Namur).

************************************************

SOCIAL EVENTS

On Wednesday afternoon, June 29th, 1994, you will be able to visit the 
MUCL Fungus Culture Collection and the exhibit hall.

The Anniversary Banquet on Wednesday evening, June 29th, will be held at 
the Villers-la-Ville Abbey Farm. 

*************************************************
TOURIST ATTRACTIONS

All major Belgian tourist cities (Brussels, Namur, Lige, Bruges, Ghent,
...) 
are easily accessible by train or car from Louvain-la-Neuve.

Information can be obtained at the railway station in Louvain-la-Neuve or
at 
MUCL. Reduced prices are offered for group and/or one-day trips. 

*************************************************
SCIENTIFIC EXHIBITIONS

Parallel to the workshop, scientifics posters will be exposed. Several 
companies will present laboratory equipments and products in the field of 
microbiology on Wednesday, June 29th, 1994.

*************************************************
TRANSPORTATION TO PRAGUE

Bruxelles-Prague-Bruxelles:

* By flight, twice a day, more or less 13.000 BEF (PEX rate for a stay 
including one Saturday-Sunday night).
* By night coach (Europabus, Bohemian Express), more or less 4.000 BEF.

Please let us know what transportation you choose. We are ready to help you

to book.
*************************************************

ORGANIZING COMMITTEE

G.L. Hennebert, J. Decallonne (UCL)
J. De Brabandere (SPPS-BCCM)
P. Charue, C. Decock, P. Evrard, N. Jamin, P. Massart, C. Moulliard, L. 
NŽlissen, S. Vanhulle (UCL-MUCL)
In collaboration with REUL - Service des Relations ExtŽrieures (UCL)


				For a registration program

		CALL, FAX, E-mail or WRITE to

Prof. Dr. G.L. Hennebert
MUCL 100 Years 1894-1994
Mycothque de l'UniversitŽ Catholique de Louvain
3 Place Croix du Sud
B-1348 Louvain-la-Neuve
Belgium

Tel. + 32-(0)10-473742 
Fax: + 32-(0)10-451501

E-Mail: Moulliard@mbla.ucl.ac.be

*************************************************

From owner-mycology@net.bio.net Wed Mar 23 22:00:00 1994
Path: biosci!agate!ihnp4.ucsd.edu!usc!yeshua.marcam.com!news.kei.com!newsstand.cit.cornell.edu!cornell!uw-beaver!netnews.nwnet.net!news.u.washington.edu!microb3.biostat.washington.edu!user
From: gosink@u.washington.edu (John Gosink)
Newsgroups: bionet.mycology
Subject: Re: morel mushrooms
Followup-To: bionet.mycology
Date: Wed, 23 Mar 1994 16:36:02 -0800
Organization: University of Washington
Lines: 11
Message-ID: <gosink-230394163603@microb3.biostat.washington.edu>
References: <Cn30AA.AuJ@lehman.com> <2modii$b7a@news.u.washington.edu> <1994Mar23.152133.60858@kuhub.cc.ukans.edu>
NNTP-Posting-Host: microb3.biostat.washington.edu

Greetings.  I have dealt with Fungi Perfecti.  They are very reputable, and
extremely attentive to customer concerns.  They are also quite prompt.  I
have not tried their outdoor morel kit, but have tried their indoor oyster,
shiitake, hericium, and nameko kits.  My first nameko kit didn't fruit. 
After
calling FP, they replaced it free of charge.  I strongly recommend them as
a company to do business with, and I think you will be satisfied.  Morels,
of course are tricky, and I have no experience with their morel kit.

Matthew Stoecker
(yes, I know a different name appears, but this is the real me)

From owner-mycology@net.bio.net Wed Mar 23 22:00:00 1994
Newsgroups: bionet.mycology
Path: biosci!agate!ihnp4.ucsd.edu!swrinde!emory!cs.utk.edu!stc06r.CTD.ORNL.GOV!ornl!mars!matis
From: matis@mars.epm.ornl.gov (Sherri Matis)
Subject: Re: morel mushrooms
Message-ID: <1994Mar24.150534.1507@ornl.gov>
Sender: usenet@ornl.gov (News poster)
Reply-To: matis@mars.epm.ornl.gov
Organization: Oak Ridge National Lab/CESAR
References: <gosink-230394163603@microb3.biostat.washington.edu>
Date: Thu, 24 Mar 1994 15:05:34 GMT
Lines: 12

Hi guys,

Could someone please post the phone # for Fungi Perfecti

I can't get to rec.gardens from this site.

BTW does anyone know when morels fruit in east Tenn. 

The weather here is about a month ahead of Penn. and the season in Pa. for 
M. esculenta begins in may. 

Sherri

From owner-mycology@net.bio.net Wed Mar 23 22:00:00 1994
Path: biosci!news.cs.umb.edu!ctc.com!news.pop.psu.edu!news.cac.psu.edu!howland.reston.ans.net!agate!darkstar.UCSC.EDU!arapaho!nathan
From: nathan@cse.ucsc.edu (Nathan J. Wilson)
Newsgroups: bionet.mycology,rec.gardens
Subject: Re: morel mushrooms
Date: 24 Mar 1994 16:49:08 GMT
Organization: UC Santa Cruz CIS/CE
Lines: 26
Message-ID: <2msga4$2au@darkstar.UCSC.EDU>
References: <Cn30AA.AuJ@lehman.com> <2modii$b7a@news.u.washington.edu> <1994Mar23.152133.60858@kuhub.cc.ukans.edu> <gosink-230394163603@microb3.biostat.washington.edu>
NNTP-Posting-Host: arapaho.cse.ucsc.edu
X-Newsreader: TIN [version 1.2 PL2]
Xref: biosci bionet.mycology:394 rec.gardens:17578

I just recently read the section on morels in Stamet's (of Fungi
Perfecti) new book "Growing Gormet and Medicinal Mushrooms" and here's
the scoop.  From a growing point of view there are two important types
of morels, yellow morels (M. esculenta I think) and black morels
(M. elata etc.).  Only the people at Morel Mountain (a private morel
growing house) have been able to successfully and reliably grow yellow
morels.  They have a patent on the process, but no one has been able
to reproduce the process (bringing into question the validity of their
patent).  Black morels, on the other hand, have had more success in
general.  However, it is still a toin toss whether a particular
culture will actually fruit.  As I remember you can get cultures
from Fungi Perfecti which you then plant somewhere outside.  You then
sit back, try to relax and hope that nature will take its course.
I have no idea if they guarantee fruitings as they do with their
wood rotters, but I would have to guess not.

As I mentioned, all this info is available in Stamets new book which is
an absolute "must have" for anyone serious about growing mushrooms.
Note this is not the same book as his previous book "The Mushroom
Cultivator".
   -------------------------    _________
         Nathan Wilson         <_________>
      nathan@cse.ucsc.edu         _|_|_       It is no dream!
      Co-Science Advisor          \___/    Matsutake are growing
   Minister of Local Forays        | |        On the belly of the mountain.
Fungus Federation of Santa Cruz    \_/ *83--                -Shigetaka

From owner-mycology@net.bio.net Thu Mar 24 22:00:00 1994
Path: biosci!bcm!cs.utexas.edu!usc!howland.reston.ans.net!EU.net!sunic!trane.uninett.no!daresbury!bioftp.unibas.ch!embl-heidelberg.de!sensen
From: sensen@embl-heidelberg.de
Newsgroups: bionet.mycology
Subject: ISEP-10
Message-ID: <1994Mar24.225617.170913@eros.embl-heidelberg.de>
Date: 24 Mar 94 22:56:17 +0100
Organization: European Molecular Biology Laboratory
Lines: 71

* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *

                            I S E P - 1 0

                        Tenth biennial meeting
          International Society for Evolutionary Protistology

                           4-10 August 1994
                         Dalhousie University
                      Halifax, Nova Scotia, Canada


                  Registration deadline:  1 July 1994
            On-campus accommodations deadline:  4 July 1994


The ISEP-10 Second (Final) Circular, with detailed information on
registration, housing and the scientific programme, is now being mailed
to all members of ISEP, and to everyone who replied to our earlier
publicity.  The ISEP-10 Secretariat will be pleased to send a copy of the
Second Circular to others upon request.

Special minisymposia, workshops and lectures at ISEP-10 will include:

   Aquatic parasitic and pathogenic protists  (4 August)
   Keynote lecture:  John O. Corliss  (5 August)
   The cytoskeleton in protist evolution: a molecular perspective (5 Aug)
   President's address:  Michael Melkonian  (8 August)
   Protein sequences as markers in protist evolution  (9 August)
   Evolution of unusual molecular processes in protists  (10 August)
   The origin of sex  (10 August)

As well, contributed papers and posters will cover diverse topics
including the origin of the eukaryotic cell, the protistan fossil
record, mitosis, new and unusual protists, and phylogenetic methods.

Confirmed speakers include D. Bhattacharya, E.U. Canning, T. Cavalier-
Smith, A. Cembella, W.F. Doolittle, J. Feagin, L.J. Goff, M.W. Gray, 
R.B. Hallick, D. Hickey, L. Hurst, M.L. Kent, L.F. Landweber, J. Lom,
L. Margulis, W. Martin, D. Maslov, D.I. Meyer, P.A.M. Michels, 
C.A. Morrison, S. McGladdery, M. Mueller, J. Pawlowski, F.O. Perkins, 
D.M. Prescott, R.J. Redfield, A. Roger, J.W. Schopf, C. Silflow, 
J. Steinkoetter, and C. Woestmann among many others.

On-campus housing is available at a very reasonable rate.  An opening
buffet/mixer will be held at the oceanfront Saraguay Club on the evening
of 4 August.  The conference banquet will be held at the Halifax Sheraton,
overlooking the historic downtown waterfront, on the evening of 8 August.

Touristic and scientific excursions are planned for Sunday, 7 August.
The extremely popular International Buskers Festival will be held 
throughout downtown Halifax from 4-14 August.

The final day of ISEP-10 (Wednesday, 10 August) also be the first day of
the 1994 meeting of the Canadian Institute for Advanced Research's Program
in Evolutionary Biology.  Both groups will meet together.

ISEP is a small but intellectually vigorous interdisciplinary soceity
bringing together all scientists interested in evolution and phylogeny of
the organisms traditionally known as protozoa, algae and fungi.  Non-
members are encouraged to attend and participate.  For further information,
contact the ISEP-10 Secretariat at:

   fax:  902-426-9413
   e-mail:  mark@imb.Lan.nrc.ca
   snail mail:  ISEP-10 Secretariat
                NRC Institute for Marine Biosciences
                1411 Oxford Street, Halifax, Nova Scotia CANADA  B3H 3Z1

* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *


From owner-mycology@net.bio.net Thu Mar 24 22:00:00 1994
Newsgroups: bionet.mycology
Path: biosci!agate!usenet.ins.cwru.edu!magnus.acs.ohio-state.edu!csn!col.hp.com!fc.hp.com!rxs
From: rxs@news.fc.hp.com (Lee Snider)
Subject: Colorado Mycological Society
Sender: news@fc.hp.com (news daemon)
Message-ID: <Cn77ns.9A8@fc.hp.com>
Date: Fri, 25 Mar 1994 02:16:40 GMT
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Greetings everyone,

I just joined the Colorado M. S. and was wondering if anyone
else out there has. I sent the dues but haven't received the
info they said they would send. I need to know when and where
the meetings are held.

Thanks,

Lee Snider


From owner-mycology@net.bio.net Thu Mar 24 22:00:00 1994
Newsgroups: bionet.mycology
Path: biosci!agate!dog.ee.lbl.gov!ihnp4.ucsd.edu!usc!yeshua.marcam.com!zip.eecs.umich.edu!newsxfer.itd.umich.edu!nntp.cs.ubc.ca!newsserver.sfu.ca!sfu.ca!rosen
From: rosen@fraser.sfu.ca (Wilf Rosenbaum)
Subject: Medicinal Properties of Mushrooms
Message-ID: <rosen.764613905@sfu.ca>
Sender: news@sfu.ca (seymour news)
Organization: Simon Fraser University, Burnaby, B.C., Canada
Date: Fri, 25 Mar 1994 16:45:05 GMT
Lines: 14

Hi,
  I am looking for evidence substantiating some claims I have heard 
  suggesting that some forms of mushrooms may provide some protection
  against various forms of cancer or other diseases. I have done a search
  on Medline but that revealed only a few studies that showed increased
  incidence of cancer among rats whose diets contained high proportion of 
  raw mushrooms. There was no indication of any study which showed any 
  protective effects. 

  Any information would be greatly appreciated. Thanks for reading this far.

  Wilf Rosenbaum
  rosen@sfu.ca
 

From owner-mycology@net.bio.net Thu Mar 24 22:00:00 1994
Newsgroups: bionet.mycology,rec.gardens
Path: biosci!agate!howland.reston.ans.net!cs.utexas.edu!swrinde!sgiblab!sgigate.sgi.com!olivea!apple.com!ntg!mboom
From: mboom@ntg.com (Mike Boom)
Subject: Re: morel mushrooms
Message-ID: <1994Mar25.182233.9354@ntg.com>
Organization: New Technologies Group, Inc.  Palo Alto CA
References: <1994Mar23.152133.60858@kuhub.cc.ukans.edu> <gosink-230394163603@microb3.biostat.washington.edu> <2msga4$2au@darkstar.UCSC.EDU>
Date: Fri, 25 Mar 1994 18:22:33 GMT
Lines: 12
Xref: biosci bionet.mycology:398 rec.gardens:17657

Nathan Wilson writes:
>Only the people at Morel Mountain (a private morel
>growing house) have been able to successfully and reliably grow yellow
>morels.

Does anyone out there know anything more about Morel Mountain, the source
of their patent, and their intentions for morel cultivation?  Stamets'
book lists their location in Mason, MI, phone #(617) 676-6373.  Any
Michiganders out there have an inkling about these guys?

Mike Boom
A tall guy looking low for fungus (let the others look high!)

From owner-mycology@net.bio.net Fri Mar 25 22:00:00 1994
Path: biosci!waite.adelaide.edu.au!pmurphy2
From: pmurphy2@waite.adelaide.edu.au (Phil Murphy)
Newsgroups: bionet.mycology
Subject: unsubcribe
Date: 25 Mar 1994 17:51:27 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 1
Sender: daemon@net.bio.net
Distribution: bionet
Message-ID: <9403260152.AA20048@schooner.waite.adelaide.edu.au>
NNTP-Posting-Host: net.bio.net

unsubscribe

From owner-mycology@net.bio.net Fri Mar 25 22:00:00 1994
Path: biosci!agate!howland.reston.ans.net!vixen.cso.uiuc.edu!news.uoregon.edu!netnews.nwnet.net!news.u.washington.edu!todell
From: todell@u.washington.edu (Thomas O'Dell)
Newsgroups: bionet.mycology
Subject: Re: Medicinal Properties of Mushrooms
Date: 25 Mar 1994 20:48:00 GMT
Organization: University of Washington, Seattle
Lines: 1
Message-ID: <2mvim0$m6m@news.u.washington.edu>
References: <rosen.764613905@sfu.ca>
NNTP-Posting-Host: carson.u.washington.edu



From owner-mycology@net.bio.net Sun Mar 27 23:00:00 1994
Newsgroups: bionet.mycology
Path: biosci!agate!howland.reston.ans.net!torn!news.unb.ca!csd.unb.ca!UNBSJ.CA!c0hx
From: c0hx@UNBSJ.CA (JASON K. DOBRANIC)
Subject: Contaminated cultures?
Message-ID: <c0hx.30.0@UNBSJ.CA>
Sender: news@jupiter.sun.csd.unb.ca
Organization: UNB Saint John Campus
Date: Mon, 28 Mar 1994 22:57:49 GMT
Lines: 4

Hi all,
	I was wondering what the best method was to get a slow growing 
fungus which has been contaminated with a fast growing fungus on an agar 
plate separated. Thanks.

From owner-mycology@net.bio.net Sun Mar 27 23:00:00 1994
Path: biosci!ABELLO.SECI.UCHILE.CL!cmartine
From: cmartine@ABELLO.SECI.UCHILE.CL
Newsgroups: bionet.mycology
Subject: (none)
Date: 28 Mar 1994 13:22:25 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 13
Sender: daemon@net.bio.net
Distribution: bionet
Message-ID: <Pine.3.87.9403281710.D9416-0100000@abello.seci.uchile.cl>
Reply-To: cmartine@abello.seci.uchile.cl
NNTP-Posting-Host: net.bio.net


Hi,

 I am in need an e-mail address for Dr. Eric A. Johnson, professor, food 
microbiology/toxicology, University of Wisconsin, Madison, USA. If anyone 
knows this information I would be greatful if you could e-mail if to me.
Thanks,
Claudio Martinez F.
Universidad de Chile.





From owner-mycology@net.bio.net Mon Mar 28 23:00:00 1994
Path: biosci!agate!howland.reston.ans.net!news.ans.net!hp81.prod.aol.net!search01.news.aol.com!not-for-mail
From: mklingler@aol.com (MKlingler)
Newsgroups: bionet.mycology
Subject: Re: Contaminated cultures?
Date: 28 Mar 1994 21:20:08 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 6
Sender: news@search01.news.aol.com
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NNTP-Posting-Host: search01.news.aol.com

In article <c0hx.30.0@UNBSJ.CA>, c0hx@UNBSJ.CA (JASON K. DOBRANIC) writes:

Hi, Jason!  What you need to do is to find out what growth conditions favor
your slow fungus.  Look at temperature, nutrients, sometimes even specific
antibiotics.  The spores can make these things hard to separate.  You just have
to try things and figure out what works for this particular fungus.  Good luck

From owner-mycology@net.bio.net Mon Mar 28 23:00:00 1994
Path: biosci!agate!howland.reston.ans.net!torn!nermal.cs.uoguelph.ca!herman.cs.uoguelph.ca!jdoherty
From: jdoherty@uoguelph.ca (John Doherty)
Newsgroups: bionet.mycology
Subject: RFD: sci.bio.mycorrhizae
Date: 29 Mar 1994 14:49:04 GMT
Organization: University of Guelph
Lines: 11
Message-ID: <2n9f50$7aa@nermal.cs.uoguelph.ca>
NNTP-Posting-Host: herman.cs.uoguelph.ca
X-Newsreader: TIN [version 1.2 PL2]

Summary: 
Keywords: 


This is an official reqest for discussion on the formation of a new
newsgroup to be tentatively called sci.bio.mycorrhizae. It would deal with
all aspects of mycorrhizal relationships and would be intended to
facilitate information exchange between researchers and other interested
parties.

Please post any comments to this thread and/or news.announce.newgroups.

From owner-mycology@net.bio.net Mon Mar 28 23:00:00 1994
Path: biosci!agate!howland.reston.ans.net!pipex!sunic!trane.uninett.no!daresbury!not-for-mail
From: SCHURENF <SCHURENF@biol.rug.nl>
Newsgroups: bionet.mycology
Subject: use of phleomycin in transformation
Date: 29 Mar 1994 14:22:52 +0100
Organization: Department of Biology, RUGroningen
Lines: 15
Sender: daemon@mserv1.dl.ac.uk
Distribution: bionet
Message-ID: <2n9a3c$ohf@mserv1.dl.ac.uk>
Original-To: mycology@dl.ac.uk

I'm working with the basidiomycete Schizophyllum commune and we are 
using the phleomycin (bleomycin) resistance system for transformation. 
If we use 0.5 M MgSO4 as osmotic stabiliser during regeneration of the 
protoplasts we obtain 1000-5000 times as many transformants compared 
to using 1M sorbitol. This is not caused by differences in 
regeneration efficiency and also not by obtaining false positives. 
However, if we try the same procedure for other basidiomycetes we do 
seem to obtain false positives. Who recognizes this from his/her own 
work or has any idea how MgSo4 interacts with phleomycin?

Frank Schuren
Dept. Plant Biology
University of Groningen
The Netherlands
e-mail: schurenf@biol.rug.nl

From owner-mycology@net.bio.net Mon Mar 28 23:00:00 1994
Path: biosci!myconet.org!christian.smith
From: christian.smith@myconet.org
Newsgroups: bionet.mycology
Subject: TECH UPDATE
Date: 29 Mar 1994 12:41:15 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 10
Sender: daemon@net.bio.net
Distribution: bionet
Message-ID: <9403291153.A2133wk@myconet.org>
NNTP-Posting-Host: net.bio.net


It would be very convenient if we were able to exchange a few basidiospores
now and then across the Internet.  Not a large number, I don't want to 
monopolize band width (heaven forbid!), but just a few so that we can 
exchange breeding material.  Maybe some of those with a molecular orientation
can let me know what might be a reasonable projected start date for such
an exchange program.  Also, I'd be interested in how one might categorize
an exchange group of this type.  Seems like something beyond a newsgroup.

                  Christian

From owner-mycology@net.bio.net Mon Mar 28 23:00:00 1994
Path: biosci!agate!howland.reston.ans.net!cs.utexas.edu!uunet!hp81.prod.aol.net!search01.news.aol.com!not-for-mail
From: meeske@aol.com (Meeske)
Newsgroups: bionet.mycology
Subject: Re: Mushroom prices
Date: 29 Mar 1994 11:14:02 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 12
Sender: news@search01.news.aol.com
Message-ID: <2n9k4a$887@search01.news.aol.com>
References: <2gunn5$m5m@eis.calstate.edu>
NNTP-Posting-Host: search01.news.aol.com

In article <2gunn5$m5m@eis.calstate.edu>, cwisnia@eis.calstate.edu (Cory M
Wisnia) writes:

Staurt: I realize I am responding a little late to your request for wild
harvested mushroom prices, but I have only recently gotten internet access. I
have historical pricing information for most wild harvested msuhrooms in the
PNW going back a few years, if you still need it. Please reply, and I will send
it to you directly. The market for wild harvested mushrooms is inefficient,
because the crops vary dramatically season to seaon and location to location,
and because the market is small. Prices fluctuate wildly, and are not uniform
world wide. The whole subject of wild harvested mushroom economics could be a
masters thesis. I would love to know how your research was accepted at school.

From owner-mycology@net.bio.net Wed Mar 30 23:00:00 1994
Path: biosci!NCCCOT2.AGR.CA!BISSETTJ
From: BISSETTJ@NCCCOT2.AGR.CA (John Bissett)
Newsgroups: bionet.mycology
Subject: Permeant vital stains for yeasts
Date: 31 Mar 1994 13:58:11 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 17
Sender: daemon@net.bio.net
Distribution: bionet
Message-ID: <01HAMMGE7PIQ005655@GW.AGR.CA>
NNTP-Posting-Host: net.bio.net

   An industry client has an urgent need of a permeant vital
   stain for Saccharomyces cerevisiae and other yeasts.  Their
   automated imaging system cannot reliably distinguish yeasts
   on grid membrane filters from dust and other
   autofluorescing contaminants.  The stain should not be
   toxic to yeasts.  A fluorescent brightener should have an
   emission range other than 415-505 nm, the range of
   autofluorescence in this application.
   
   Has anyone knowledge of vital stains/optical brighteners
   for this application - or another solution?
   
   Please address replies to:   BISSETTJ@NCCCOT2.AGR.CA
   
   Thanks
   
   John Bissett

From owner-mycology@net.bio.net Wed Mar 30 23:00:00 1994
Path: biosci!daresbury!not-for-mail
From: "L.J.L.D." Van Griensven <mushex!vg@relay.NL.net>
Newsgroups: bionet.mycology
Subject: Re: Compost spores and health.
Date: 30 Mar 1994 17:29:14 +0100
Lines: 40
Sender: daemon@mserv1.dl.ac.uk
Distribution: bionet
Message-ID: <2nc9cq$en9@mserv1.dl.ac.uk>
Original-Received: by NeXT Mailer 
                   (1.95)
PP-warning: Illegal Received field on preceding line
Original-To: london@SunSite.unc.edu (Larry London),
 mycology@dl.ac.uk

Compost spores and Health

For those interested in possible health effects of mold spores  
associated with composting operations and present in air:  We have  
done a study of the etiology of mushroom worker's lung with the  
following results.
The spores of the thermophilic actinomycetes Excellospora flexuosa,  
Thermomonospora alba, T. curvata and T. fusca  were collected from  
the air in compost-fermentation tunnels during the spawning of  
mushroom compost.  They were found present at over 10 power 9 CFU per  
cubic meter of air. ( This is 10 times higher than needed for  
pathogenesis)
Aspergillus, Aureobasidium, Cladosporium, Penicillium and Scytalidium  
were found at only 10 power 3 spores per cubic meter of air.  Sera of  
10 mushroom growers with MWL were all found positive for one or more  
of the 4 actinomycetes by dot ELISA against spore antigens. No  
antibodies were found against Streptomyces vulgaris,  
Thermoactinomyces vulgaris, T. sacchari and Trichoderma viride.  Sera  
of 11 of 14 workers engaged in handling  Phase 2 compost reacted  
positively with 1 or more of the actinomyces. Their serum titers  
increased with the duration of employment. The sera of 19 non-exposed  
workers were negative. Results of inhalation provocation studies  
showed a causal relationship between spore exposition and disease.
The study was published in Mycopathologia 122: 21-28 (1993). Mushroom  
compost is very much like any other compost and can therefore be  
considered as a model. 

Influence of spores coming from composting plants through the air to  
bone marrow transplant patients seems unlikely. Compost contains  
mostly thermophilic actinomycetes. After longer incubation at 45 C,  
thermophilic fungi will be found. A. fumigatus was never observed by  
us in mushroom compost.
Tunnels contain 100 tons of compost. Simple calculation shows the  
unlikeliness of  contamination by compost derived fungal spores  
through air. Besides, bone marrow patients are usually isolated in an  
environment, that is made free of the microorganisms that are  
abundantly available in the air of hospitals.

Leo Van Griensven
Mushroom Experimental Station, Horst, The Netherlands.

From owner-mycology@net.bio.net Thu Mar 31 23:00:00 1994
Newsgroups: bionet.mycology
Path: biosci!agate!ihnp4.ucsd.edu!swrinde!emory!news-feed-2.peachnet.edu!umn.edu!news
From: brambl@graz.cbs.umn.edu (Robert Brambl)
Subject: Re: Permeant vital stains for yeasts
Message-ID: <CnLABB.1tn@news.cis.umn.edu>
Sender: news@news.cis.umn.edu (Usenet News Administration)
Nntp-Posting-Host: graz.cbs.umn.edu
Reply-To: brambl@molbio.cbs.umn.edu (Robert Brambl)
Organization: University of Minnesota, Twin Cities
References: <01HAMMGE7PIQ005655@GW.AGR.CA>
Distribution: bionet
Date: Fri, 1 Apr 1994 16:42:18 GMT
Lines: 33

In article <01HAMMGE7PIQ005655@GW.AGR.CA> BISSETTJ@NCCCOT2.AGR.CA (John  
Bissett) writes:
>    An industry client has an urgent need of a permeant vital
>    stain for Saccharomyces cerevisiae and other yeasts.  Their
>    automated imaging system cannot reliably distinguish yeasts
>    on grid membrane filters from dust and other
>    autofluorescing contaminants.  The stain should not be
>    toxic to yeasts.  A fluorescent brightener should have an
>    emission range other than 415-505 nm, the range of
>    autofluorescence in this application.
>    
>    Has anyone knowledge of vital stains/optical brighteners
>    for this application - or another solution?
>    
>    Please address replies to:   BISSETTJ@NCCCOT2.AGR.CA
>    
>    Thanks
>    
>    John Bissett

An excellent source for a large variety of dyes is a company called  
Molecular Probes (Eugene, Oregon).  A group of dyes you should consider  
are the potentiometric cyanine dyes, some of which report the energization  
of the mitochondrial membrane and which are easily measured with  
fluorescence microscopy. Others may report pH of the cytosol.  One of  
these which works really well for fungi is DiOC7(3), the full name of  
which either Molecular Probes or I can provide, if necessary. If  
autofluorescence remains a problem with your measurements, this could be  
subtracted by treatment of the yeast cell samples with CCCP or DCCD. 

Hope this helps.

R. Brambl

