From owner-mycology@net.bio.net Fri Apr 01 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!agate!howland.reston.ans.net!sol.ctr.columbia.edu!news.columbia.edu!psinntp!psinntp!psinntp!newstand.syr.edu!rodan.syr.edu!jworrall From: jworrall@rodan.syr.edu (James J. Worrall) Subject: Re: Contaminated cultures? Message-ID: <1994Apr1.170406.4823@newstand.syr.edu> Sender: netnews@newstand.syr.edu (Network News Administrator) Nntp-Posting-Host: rodan.syr.edu Organization: Syracuse University, Syracuse, NY References: Date: Fri, 1 Apr 1994 17:04:06 GMT Lines: 8 To rescue your slow growing fungus, you might: a) hyphal-tip it (requires good technique and tools) b) transfer some conidia with a needle if it forms conidia c) use selective fungicides to inhibit the fast one. The choice of techniques depends on the fungi involved. From owner-mycology@net.bio.net Fri Apr 01 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!torn!news.unb.ca!coranto.ucs.mun.ca!calvin.stemnet.nf.ca!rdunphy From: rdunphy@calvin.stemnet.nf.ca (Richard A. Dunphy) Newsgroups: bionet.mycology Subject: wanted Date: 2 Apr 1994 20:51:52 GMT Organization: Memorial University of Newfoundland Lines: 9 Message-ID: <2nklt8$64k@coranto.ucs.mun.ca> NNTP-Posting-Host: calvin.stemnet.nf.ca X-Newsreader: TIN [version 1.2 PL2] I am a novice in the growing of mushrooms. I would like to know where I could purchase mushroom culture as well as information on growing the same. I live in Newfoundland,Canada and would like to purchase from a dealer as close as possible to here. Dick Dunphy P.O. Box 257 Dunville,Placentia AOB1SO rdunphy@calvin.stemnet.nf.ca From owner-mycology@net.bio.net Sat Apr 02 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!usc!crash!news.sprintlink.net!connected.com!connected.com!awebster From: awebster@hebron.connected.com (Al Webster) Newsgroups: bionet.mycology Subject: Re: morel mushrooms Date: 3 Apr 1994 10:07:38 -0700 Organization: Connected INC -- Internet Services Lines: 17 Message-ID: References: <1994Mar24.150534.1507@ornl.gov> NNTP-Posting-Host: hebron.connected.com The number for Fungi Perfecti is 206-426-9292. matis@mars.epm.ornl.gov (Sherri Matis) writes: >Hi guys, >Could someone please post the phone # for Fungi Perfecti >I can't get to rec.gardens from this site. >BTW does anyone know when morels fruit in east Tenn. >The weather here is about a month ahead of Penn. and the season in Pa. for >M. esculenta begins in may. >Sherri From owner-mycology@net.bio.net Sat Apr 02 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!agate!usenet.ins.cwru.edu!magnus.acs.ohio-state.edu!csn!col.hp.com!fc.hp.com!rxs From: rxs@news.fc.hp.com (Lee Snider) Subject: mycorrhizae Sender: news@fc.hp.com (news daemon) Message-ID: Date: Sun, 3 Apr 1994 07:28:52 GMT Nntp-Posting-Host: hpfire0.fc.hp.com Organization: Hewlett-Packard Fort Collins Site X-Newsreader: TIN [version 1.2 PL1.4] Lines: 7 As an interested party, I would enjoy seeing a sci.bio.mycorrhizae group. Lee Snider rxs@hpfidlf.fc.hp.com From owner-mycology@net.bio.net Sun Apr 03 23:00:00 1994 Path: biosci!BULL.CC.USM.EDU!GLEN_SHEARER From: GLEN_SHEARER@BULL.CC.USM.EDU Newsgroups: bionet.mycology Subject: (none) Date: 4 Apr 1994 13:21:46 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <199404042020.PAA02620@darban.cc.usm.edu> NNTP-Posting-Host: net.bio.net DATA Date: Mon, 04 Apr 94 15:19 CDT From: GLEN SHEARER To: mycology@net.bio.NET Really-to: mycology@net.bio.net Subject: yeast transformation Message-ID: <940404.15221932.050131@USM.CP6> What is a reasonable expectation for the number of Saccharomyces cerevisiae transformants from 1 ug of a 2 micron containing plasmid with the LiAc method? I've heard that the protoplast method is "magic" i.e., sometimes it works and sometimes not. Any tips appreciated! Thanks Glen From owner-mycology@net.bio.net Sun Apr 03 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!agate!ihnp4.ucsd.edu!sdd.hp.com!vixen.cso.uiuc.edu!uwm.edu!news.doit.wisc.edu!psl.wisc.edu!news From: MULROY@EPISYS.EPI.WISC.EDU (Bob Mulroy) Subject: Re: PROPOSAL: sci.ethnobiology In-Reply-To: boe666@u.washington.edu's message of 4 Apr 1994 01:16:29 GMT Message-ID: <1994Apr4.192928.18338@pslu1.psl.wisc.edu> Sender: news@pslu1.psl.wisc.edu (USENET News System) Organization: University of Wisconsin/Sleep Research Lab References: <2n57bt$5dr@news.u.washington.edu> <2nnppd$e5o@news.u.washington.edu> Date: Mon, 4 Apr 94 19:29:28 GMT X-News-Reader: VMS NEWS 1.24 Lines: 5 Count me in! Bob Mulroy, A keen gardner, decent dad/mom, Dir. of the above org'd lab. 9-A University Houses, Madison WI 53705. (608) 233-4672 Bob in '96!!! From owner-mycology@net.bio.net Sun Apr 03 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!torn!nermal.cs.uoguelph.ca!herman.cs.uoguelph.ca!lmelvill From: lmelvill@uoguelph.ca (Lewis Melville) Newsgroups: bionet.mycology Subject: Re: Permeant vital stains for yeasts Date: 4 Apr 1994 17:38:40 GMT Organization: University of Guelph Lines: 23 Distribution: bionet Message-ID: <2npjb0$5vt@nermal.cs.uoguelph.ca> References: <01HAMMGE7PIQ005655@GW.AGR.CA> NNTP-Posting-Host: herman.cs.uoguelph.ca X-Newsreader: TIN [version 1.2 PL2] Robert Brambl (brambl@graz.cbs.umn.edu) wrote: : In article <01HAMMGE7PIQ005655@GW.AGR.CA> BISSETTJ@NCCCOT2.AGR.CA (John : Bissett) writes: : > An industry client has an urgent need of a permeant vital : > stain for Saccharomyces cerevisiae and other yeasts. Their : > John Bissett : An excellent source for a large variety of dyes is a company called : Molecular Probes (Eugene, Oregon). A group of dyes you should consider : are the potentiometric cyanine dyes, some of which report the energization : of the mitochondrial membrane and which are easily measured with : fluorescence microscopy. Others may report pH of the cytosol. One of : these which works really well for fungi is DiOC7(3), the full name of : which either Molecular Probes or I can provide, if necessary. If : autofluorescence remains a problem with your measurements, this could be : subtracted by treatment of the yeast cell samples with CCCP or DCCD. : Hope this helps. : R. Brambl You might also check out a paper on vital staining using Nitro blue Tetrazolium, by G. Schaffer in Mycorrhiza 4: 29-35 (1993). lewis melville. From owner-mycology@net.bio.net Sun Apr 03 23:00:00 1994 Path: biosci!PCLSP2.KUICR.KYOTO-U.AC.JP!marek From: marek@PCLSP2.KUICR.KYOTO-U.AC.JP (Marek Tchorzewski) Newsgroups: bionet.mycology Subject: N.crassa Genomic DNA Date: 3 Apr 1994 21:41:22 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <9404040443.AA03651@pclsp2> NNTP-Posting-Host: net.bio.net Hello Netters, I have just started working on Neurospora crassa, so I am completely new on this field. Well, what I need, is suitable protocol for genomic DNA isolation from Neurospora crassa. I appreciate very much any suggestion in this matter. Marek From owner-mycology@net.bio.net Sun Apr 03 23:00:00 1994 Path: biosci!PCLSP2.KUICR.KYOTO-U.AC.JP!marek From: marek@PCLSP2.KUICR.KYOTO-U.AC.JP (Marek Tchorzewski) Newsgroups: bionet.mycology Subject: N.crassa Genomic DNA Date: 3 Apr 1994 21:40:29 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <9404040443.AA03647@pclsp2> NNTP-Posting-Host: net.bio.net From owner-mycology@net.bio.net Sun Apr 03 23:00:00 1994 Path: biosci!daresbury!trane.uninett.no!sunic!pipex!howland.reston.ans.net!torn!news.unb.ca!coranto.ucs.mun.ca!calvin.stemnet.nf.ca!rdunphy From: rdunphy@calvin.stemnet.nf.ca (Richard A. Dunphy) Newsgroups: bionet.mycology Subject: fungi perfecti Date: 4 Apr 1994 17:50:29 GMT Organization: Memorial University of Newfoundland Lines: 8 Message-ID: <2npk15$cl4@coranto.ucs.mun.ca> NNTP-Posting-Host: calvin.stemnet.nf.ca X-Newsreader: TIN [version 1.2 PL2] Does anybody have the address and phone number of Fungi Perfecti in Washington? -- Dick Dunphy P.O. Box 257 Dunville,Placentia AOB1SO rdunphy@calvin.stemnet.nf.ca From owner-mycology@net.bio.net Sun Apr 03 23:00:00 1994 Path: biosci!ABELLO.SECI.UCHILE.CL!cmartine From: cmartine@ABELLO.SECI.UCHILE.CL Newsgroups: bionet.mycology Subject: Re: N.crassa Genomic DNA Date: 4 Apr 1994 15:54:15 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Distribution: bionet Message-ID: References: <9404040443.AA03651@pclsp2> NNTP-Posting-Host: net.bio.net On 3 Apr 1994, Marek Tchorzewski wrote: > > Hello Netters, > > I have just started working on Neurospora crassa, so I am > completely new on this field. > > Well, what I need, is suitable protocol for genomic DNA > isolation from Neurospora crassa. > > I appreciate very much any suggestion in this matter. > > Marek > > Hi Marek, for genomic DNA isolation from N. crassa, I used the following protocol: Cifuentes et al, (1990) Fungal Genetics Newsletter 37:12. Claudio From owner-mycology@net.bio.net Mon Apr 04 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!bcm!cs.utexas.edu!swrinde!gatech!newsxfer.itd.umich.edu!nntp.cs.ubc.ca!utcsri!newsflash.concordia.ca!CC.UMontreal.CA!IRO.UMontreal.CA!clouso.crim.ca!athena.ulaval.ca!dietcola.rsvs.ulaval.ca!user From: lsimon@alnus.for.ulaval.ca (Luc Simon) Subject: Re: RFD: sci.bio.mycorrhizae Content-Type: text/plain; charset=ISO-8859-1 Message-ID: Followup-To: bionet.mycology Sender: news@athena.ulaval.ca Nntp-Posting-Host: dietcola.rsvs.ulaval.ca Content-Transfer-Encoding: 8bit Organization: Universite Laval References: <2n9f50$7aa@nermal.cs.uoguelph.ca> Mime-Version: 1.0 Date: Tue, 5 Apr 1994 19:56:05 GMT Lines: 34 In article <2n9f50$7aa@nermal.cs.uoguelph.ca>, jdoherty@uoguelph.ca (John Doherty) wrote: > Summary: > Keywords: > > > This is an official reqest for discussion on the formation of a new > newsgroup to be tentatively called sci.bio.mycorrhizae. It would deal with > all aspects of mycorrhizal relationships and would be intended to > facilitate information exchange between researchers and other interested > parties. > > Please post any comments to this thread and/or news.announce.newgroups. Dear John, Even if I would very much like to read news dealing with mycorrhizae, I think that the creation of a new group, especially in the sci.bio hierarchy is the way to go. Why not instead invest the already existing bionet.mycology newsgroup? The traffic is still low in this group, and I suggest that the creation of a mycorrhizae-specific newsgroup be deferred until the number of postings to the bionet.mycology newsgroup justifies spliting it up. Another advantage of using the bionet hierarchy is that we can then benefit from their archiving and support services. -- Luc Simon Universite Laval Sainte-Foy, Que lsimon@rsvs.ulaval.ca From owner-mycology@net.bio.net Mon Apr 04 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!agate!howland.reston.ans.net!cs.utexas.edu!sdd.hp.com!saimiri.primate.wisc.edu!news.doit.wisc.edu!psl.wisc.edu!news From: MULROY@EPISAS.EPI.WISC.EDU (Bob Mulroy) Subject: Re: Portobello Mushrooms In-Reply-To: cottrill@nexus.yorku.ca's message of Tue, 5 Apr 1994 06:01:24 GMT Message-ID: <1994Apr5.185854.7721@pslu1.psl.wisc.edu> Sender: news@pslu1.psl.wisc.edu (USENET News System) Organization: University of Wisconsin/Sleep Research Lab References: Date: Tue, 5 Apr 94 18:58:54 GMT X-News-Reader: VMS NEWS 1.24 Lines: 12 In cottrill@nexus.yorku.ca writes: > > Last week I bought some of these at a supermarket. I can't find " > portobello" in any of my refernce books. Could anyone tell me the > scientific name of this fungus? Is it a type of agaricus mushroom? > Yes, the grower I spoke to said it was /A. Brunescens/ although they don't key out in my books either. Bob Mulroy, A keen gardner, decent dad/mom, Dir. of the above org'd lab. 9-A University Houses, Madison WI 53705. (608) 233-4672 Bob in '96!!! From owner-mycology@net.bio.net Mon Apr 04 23:00:00 1994 Path: biosci!WVNVM.WVNET.EDU!INVAM From: INVAM@WVNVM.WVNET.EDU ("Bentivenga, Stephen P") Newsgroups: bionet.mycology Subject: Postdoc Position Date: 5 Apr 1994 08:02:31 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <199404051502.IAA02531@net.bio.net> NNTP-Posting-Host: net.bio.net A postdoctoral position is available immediately (starting date flexible) for a fungal molecular biologist. Please email me directly for information. Stephen P. Bentivenga INVAM @ WVNVM.WVNET.EDU West Virginia University From owner-mycology@net.bio.net Mon Apr 04 23:00:00 1994 Path: biosci!agate!ihnp4.ucsd.edu!munnari.oz.au!metro!grivel!metz.une.edu.au!rcorneli From: rcorneli@metz.une.edu.au (Rebecca Cornelius) Newsgroups: bionet.mycology,sci.med Subject: Studying effects of tenuazonic acid Keywords: tenuazonic acid, onyalai Message-ID: <4117@grivel.une.edu.au> Date: 29 Mar 94 00:42:11 GMT Sender: usenet@grivel.une.edu.au Followup-To: bionet.mycology, sci.med, poster Lines: 28 Nntp-Posting-Host: metz.une.edu.au Xref: biosci bionet.mycology:427 sci.med:33466 Hullo everyone, after several years as a lurker I finally have an account that will let me mail. I am in my honours year at the University of New England in Armidale, NSW, Australia and am investigating bacterial resistance to the mycotoxin tenuazonic acid. Tenuazonic acid is a secondary metabolite produced by a wide variety of fungii, particularly Alternaria alternata. The toxin has been implicated in the haematolgoical disorder onyalai in Africa and has been found to inhibit protein synthesis in mammals. Tenuazonic acid has also been shown to be involved in rice-blast disease and tobacco brown spot disease. I am intending to examine bacterial resistance, firstly by using Bacillis megaterium followed by field samples. If resistance is found (and I hope it is or my project disappears rapidly :-) ) I intend to find what is causing the resistance and then, depending on time, isolated the gene. If anyone has any information on tenuazonic acid, its effects (especially in the aetiology of onyalai) could they please mail me. I am particularly interested in a paper in the Journal of Medicinal Chemistry,(1965) vol 8, pg 483 which is proving difficult to get hold of so if anyone can help it would be greatly appreciated. Thankyou! Rebecca (Bec) Cornelius rcorneli@metz.une.edu.au From owner-mycology@net.bio.net Mon Apr 04 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!agate!howland.reston.ans.net!torn!newshub.ccs.yorku.ca!cottrill From: cottrill@nexus.yorku.ca (Ed Cottrill) Subject: Portobello Mushrooms Message-ID: Sender: news@newshub.ccs.yorku.ca (USENET News System) Organization: York University Date: Tue, 5 Apr 1994 06:01:24 GMT Lines: 5 Last week I bought some of these at a supermarket. I can't find " portobello" in any of my refernce books. Could anyone tell me the scientific name of this fungus? Is it a type of agaricus mushroom? From owner-mycology@net.bio.net Mon Apr 04 23:00:00 1994 Path: biosci!agate!darkstar.UCSC.EDU!arapaho!nathan From: nathan@cse.ucsc.edu (Nathan J. Wilson) Newsgroups: bionet.mycology Subject: Fungi Identification Database Announcement Date: 5 Apr 1994 05:27:56 GMT Organization: UC Santa Cruz CIS/CE Lines: 61 Message-ID: <2nqsss$mq1@darkstar.UCSC.EDU> NNTP-Posting-Host: arapaho.cse.ucsc.edu X-Newsreader: TIN [version 1.2 PL2] This is to announce the imminent release of "Taxy 0.0", the first version of my fungi identification database system for free public consumption. The goal of this announcement is to attract interest in the system and hopefully to extend the group of volunteers who will be helping to expand the existing database. The subsequent versions of the system and its associated databases will remain freely available in keeping with the goal of the project to create a system for sharing fungal information freely and to permit easy database expansion and interchange by interested users. The project is part of my computer science masters thesis. Expected release date for anonymous ftp: Monday April 11 For people near Santa Cruz, California there will be an introductory class on the system Saturday April 9. Anyone interested in participating should contact me directly. The first release will include: - A Taxy database including a small amount of information for a large number of species (>3100) and a nearly complete set of macroscopic information for about 50 species. - Support for creating, browsing, editing and merging Taxy databases. Taxy is designed to support any type of taxonomic information so, for example, it should be possible for an interested person to create a database of bird or plant descriptions. - Documentation on how to use the system and of the current database file format. - Executables for the Apple Macintosh, Sun SPARC, and IBM PCs. (Source code is available upon request, though I would prefer to wait for the next release to distribute source unless you are interested in actually helping to extend the system.) The first release will *not* (but the second release will) include: - A useful searching facility for actually doing identification with the system. This part of the project is still under construction. - Features for describing the microscopic aspects of fungi and a more complete treatment of features related to Ascomycetes and Gasteromycetes. (Although the truly ambitious could add these features themselves.) - A general scripting system for creating database specific commands for entering information. If you are interested in participating in any way please drop me a note or call me at: (408)423-3773. I will send out an additional announcement when the system is available for anonymous ftp. ------------------------- _________ Nathan Wilson <_________> nathan@cse.ucsc.edu _|_|_ It is no dream! Co-Science Advisor \___/ Matsutake are growing Minister of Local Forays | | On the belly of the mountain. Fungus Federation of Santa Cruz \_/ *83-- -Shigetaka From owner-mycology@net.bio.net Mon Apr 04 23:00:00 1994 Path: biosci!ADMIN.OGI.EDU!msachs From: msachs@ADMIN.OGI.EDU (Matthew Sachs) Newsgroups: bionet.mycology Subject: Re: N.crassa Genomic DNA Date: 5 Apr 1994 13:30:48 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 100 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <9404052031.AA26330@admin.ogi.edu> NNTP-Posting-Host: net.bio.net Neurospora crassa DNA mini prep (salt-detergent and TCA/EtOH) based on previous procedures; protocol written by Michael Freitag, in my laboratory (freitag@admin.ogi.edu). (1) grow standing cultures in 25 or 50 ml of 1x Vogel's with 1.5% sucrose in 125 or 250 ml Erlenmeyer flasks at RT for 2 or 4 days (yields ca. 1.5 g wet mycelial weight; avoid aerial hyphae and onset of conidiation) (2) harvest cells onto Whatman filter paper (# 1 or 541) in Buchner funnel, determine wet weight, transfer into 15 ml Falcon (#2059) tubes (or equivalent, spatula must fit in bottom), freeze -80o C for at least 20 min (3) lyophilize overnight, pulverize (vortex with spatula for ca. 30 sec) (4) suspend samples in salt-detergent solution by adding enough liquid (usually 1 ml for 1 g wet weight) to make a thick suspension after vortexing at high speed for 20 sec (5) incubate at RT for 20 min while mixing end-over-end on rotator (6) spin in Sorvall SS-34 at 8000 rpm for 10 min, this usually yields 600 ul of spnt (7) collect clear, slightly orange spnt and transfer 300 ul per Eppendorf tube (8) add 1.2 ml of TCA/EtOH and mix gently by inversion (9) precipitate at -20oC for at least 30 min (may be stored at this stage) (10) pellet nucleic acids by 15 sec microfuge spin, aspirate spnt, wash pellet with 300 ul of 70% EtOH, spin for 15 sec, aspirate spnt, dry in speed vac (11) resuspend nucleic acids in 100 ul of 10 mM NH4OAc, mix gently (it takes some time for pellets to dissolve); add 100 ul of 0.3 mg/ml RNase A in 10 mM NH4OAc, mix by pipetting up and down, incubate at 50o C for 1 h, every 15 min vortex gently to resuspend pellet (12) add 200 ul of chloroform, vortex, spin in microfuge for 5 min, transfer spnt to new tube (at this point spnts of two tubes may be combined) (13) add 107 ul of 7.5 M NH4OAc, then 0.8 ml isopropanol, mix well by inversion, spin immediately in microfuge for 25 sec (later precipitate is mostly unwanted junk), aspirate spnt, wash pellet with 300 ul of 70% EtOH, aspirate spnt, briefly dry in speed vac (if dried too hard DNA ressuspends only poorly) (15) resuspend pellet in 100 ul TE buffer overnight at 4o C (16) yields 40 to 90 ug of genomic DNA per culture, digest to yield 1 to 2 ug of DNA per lane SALT DETERGENT SOLUTION - dissolve in this order and completely: Na Deoxycholate 2.00 g Brij 58 (polyoxyethylene20cetyl ether) 5.00 g NaCl 58.44 g in 350 ml H2O - adjust to 500 ml, store at 4o C TCA/ EtOH SOLUTION - make 4.5 M TCA by dissolving 41.7 g NaTCA salt (Aldrich #19,078-0) in H2O until 50 ml are reached (density is 1.43 g/ml, i.e. wt of 50 ml should be 71.5 g) - add 50 ml EtOH, store at 4o C (precipitate will appear upon mixing and settles; don't worry, but don't mix it with the clear solution when using) RNase SOLUTION - 10 mg/ml RNase stock is diluted into 10 mM NH4OAc to yield 0.3 mg/ml RNase A (30 ul RNase A for 1ml of 10 mM NH4OAc) References: 1. Oakley, C.E. et al. 1987. Cloning of the ribo B locus of Aspergillus nidulans. Gene 53:293-298. 2. Summerton et al. 1983. A rapid method for preparation of bacterial plasmids. Anal. Biochem. 133:70-84 3. pers. commun. E. Selker lab, University of Oregon 4. pers. commun. Zongli Luo, Matthew S. Sachs and Duane T. Mooney, OGI From owner-mycology@net.bio.net Mon Apr 04 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!agate!howland.reston.ans.net!sol.ctr.columbia.edu!newsxfer.itd.umich.edu!zip.eecs.umich.edu!umn.edu!news From: brambl@graz.cbs.umn.edu (Robert Brambl) Subject: Re: N.crassa Genomic DNA Message-ID: Sender: news@news.cis.umn.edu (Usenet News Administration) Nntp-Posting-Host: graz.cbs.umn.edu Reply-To: brambl@molbio.cbs.umn.edu (Robert Brambl) Organization: University of Minnesota, Twin Cities References: <9404040443.AA03651@pclsp2> Distribution: bionet Date: Tue, 5 Apr 1994 19:23:45 GMT Lines: 24 In article <9404040443.AA03651@pclsp2> marek@PCLSP2.KUICR.KYOTO-U.AC.JP (Marek Tchorzewski) writes: > > Hello Netters, > > I have just started working on Neurospora crassa, so I am > completely new on this field. > > Well, what I need, is suitable protocol for genomic DNA > isolation from Neurospora crassa. > > I appreciate very much any suggestion in this matter. > > Marek You should evaulate the procedure refined and/or developed by the Metzenberg lab. It works well for preparation of many dozens of samples of high quality DNA simultaneously from diverse types of mutant strains. The crucial point is that the unfrozen samples *must* be thoroughly dried according to instructions. Reference: Neurospora Newsletter (1981) 28:20 and (1982) 29: 27. This lab also prepared an unpublished 1989 update and synopsis which you could obtain by writing to Dr. Robert L. Metzenberg; Department of Biomolecular Chemistry; University of Wisconsin; 1300 University Avenue; Madison, Wisconsin 53706 (Telephone (608) 262-1507. From owner-mycology@net.bio.net Tue Apr 05 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!cs.utexas.edu!swrinde!sgiblab!sgigate.sgi.com!olivea!decwrl!decwrl!waikato!waikato.ac.nz!aeiham From: aeiham@waikato.ac.nz Newsgroups: bionet.mycology Subject: Re: RFD: sci.bio.mycorrhizae Message-ID: <1994Apr6.200312.27277@waikato.ac.nz> Date: 6 Apr 94 20:03:12 +1300 References: <2n9f50$7aa@nermal.cs.uoguelph.ca> Organization: University of Waikato, Hamilton, New Zealand Lines: 25 In article <2n9f50$7aa@nermal.cs.uoguelph.ca>, jdoherty@uoguelph.ca (John Doherty) writes: > This is an official reqest for discussion on the formation of a new > newsgroup to be tentatively called sci.bio.mycorrhizae. It would deal with > all aspects of mycorrhizal relationships and would be intended to > facilitate information exchange between researchers and other interested > parties. Given the probably fairly small user base for such a group (not because there aren't many people doing mycorrhizae research, but because not many of them would use NEWS), I wonder if a group for all root / rhizosphere fungi might not be more likely to attract a viable population. Some people would argue that there's a continum from root rots to mycorrhizae anyway. Of course there's already bionet.mycology, that's fairly quiet and could probably accomodate as many mycorrhizae threads as your likely to get in sci.bio.mycorrhizae, but I assume there are distribution issues? So, I'd be more in favour of sci.bio.root-fungi, so as not to split the audience too much. Of course if you don't object to the bionet hierachy, you could look at bionet.mycology.root-fungi (or mycorrhiza), but the volume in bionet.mycology itself probably wouldn't justify the further sub-division. Cheers -tb From owner-mycology@net.bio.net Wed Apr 06 23:00:00 1994 Path: biosci!ASRR.ARSUSDA.GOV!rproctor From: rproctor@ASRR.ARSUSDA.GOV ("ROBERT PROCTOR") Newsgroups: bionet.mycology Subject: homologous recombination in fungi Date: 7 Apr 1994 14:00:27 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <199404072100.OAA11287@net.bio.net> NNTP-Posting-Host: net.bio.net Hello: Does anyone out there know anything about the frequency of homologous recombination during fungal transformation varying with the size of the piece of the target gene in the disruption vector? I seem to remember hearing a talk where someone disrupted a fungal gene with several different sized fragments of the same target gene, the larger the fragment of the target gene in the transformation vector the greater the frequency of disruptants among the transformants. If anyone has seen any published work on this, could you provide me with a reference or two? If you've had any personal, non-published experiences like this I'd be glad to hear about them too. Thanks in advance for your help. The reason I'm asking is that I disrupted a toxin biosynthetic gene, Tri6, in Fusarium sporotrichioides using the procedure in which the isolated coding region of the gene was truncated at both ends, ligated into a transformation vector, and then transformed into Fusarium. The frequency of disruptants (homologous recombination) among the transformants was quite low, about 1%. When we have disrupted other genes in F. sporotrichioides using this same approach, homologous recombination frequencies are higher, sometimes as high as 40%. One difference between these other genes and Tri6 is size. Tri6 is quite small and the truncated version used in the transformation vector was only 468 bp. The truncated versions of the other genes were about 1 kb, sometimes greater. So, my thought was that maybe the homologous recombination frequency for Tri6 was low because of the small size of the truncated copy of Tri6 in the transformation vector. Does this sound plausible? Thanks again. Bob From owner-mycology@net.bio.net Thu Apr 07 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!europa.eng.gtefsd.com!news.uoregon.edu!gaia.ucs.orst.edu!news.reed.edu!tkim From: tkim@reed.edu. (tkim) Newsgroups: bionet.mycology Subject: Slime mold stalk cells Date: 8 Apr 1994 10:01:55 GMT Organization: Reed College, Portland, Oregon Lines: 16 Message-ID: <2o3a2j$h25@scratchy.reed.edu> NNTP-Posting-Host: 134.10.5.248 X-Posted-From: InterNews 1.0@scratchy.reed.edu. I am very curious to find out when do the stalk cells of Dictyostelium discoideum die. I have been browsing through various books and journals, but couldn't find anything in terms of the stalk cell death and histochemical and ultrastructural observation of stalk cells along with the release of cellulose and cytoplasm. Can anyone help me with this question of when the stalk cells actually die? I'd appreciate any information regarding this matter. Thank you for your time! Regards, Tae Hoon Kim tkim@reed.edu From owner-mycology@net.bio.net Thu Apr 07 23:00:00 1994 Path: biosci!PHIBRED.COM!duvickj From: duvickj@PHIBRED.COM Newsgroups: bionet.mycology Subject: Sender's address missing on postings Date: 8 Apr 1994 08:55:49 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <9404081521.AA12972@phibred.phibred.com> NNTP-Posting-Host: net.bio.net I'd like to point out that certain local Internet servers (including ours) truncate the From: address line to simply "BIOSCI-REQUEST@net.bio.net", making it impossible to identify the sender. Therefore it would be helpful if senders could remember to include E-mail address information in the body of the posting. Thanks! Jon Duvick Pioneer Hi-Bred Duvickj@phibred.com From owner-mycology@net.bio.net Fri Apr 08 23:00:00 1994 Path: biosci!agate!darkstar.UCSC.EDU!ucscb.UCSC.EDU!taiga From: taiga@ucscb.UCSC.EDU (Daniel Jason Katz) Newsgroups: bionet.mycology Subject: fungus in sustainable agriculture? Date: 9 Apr 1994 18:39:18 GMT Organization: University of California, Santa Cruz Lines: 9 Message-ID: <2o6som$s97@darkstar.UCSC.EDU> NNTP-Posting-Host: ucscb.ucsc.edu Summary: Does anyone know of or do work in integrating mushroom cultivation with Keywords: agroecology, mushrooms X-Newsreader: NN version 6.5.0 #3 (NOV) Hi, I'm an undergraduate at UC Santa Cruz and I'm really interested in the idea of using fungus/mushroom cultivation in a small scale agriculture/permaculture system (ala Stamets new book). Does anyone out there do work in this field or know of people that do. I'd love to get involved in some sort of internship with a person or agency studying this. Thanks a lot! -Daniel Katz taiga@cats.ucsc.edu From owner-mycology@net.bio.net Sat Apr 09 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!agate!ihnp4.ucsd.edu!sdd.hp.com!caen!malgudi.oar.net!mercury.wright.edu!msmith From: msmith@discover.wright.edu (Mike Smith) Subject: Re: Portobello Mushrooms Message-ID: Sender: news@mercury.wright.edu (USENET News System) Organization: Wright State University, Dayton, OH 45435 X-Newsreader: TIN [version 1.2 PL2] References: Date: Sun, 10 Apr 1994 16:28:13 GMT Lines: 20 Bob Lucas (rnlucas+@cs.cmu.edu) wrote: : I believe this is the grown-up version of agaricus bisporus. We also : had trouble finding it; Mushroom Magazine did a little article on it. : I haven't checked any spore basidia to see if it is actually "bi". : Elizabeth Barrow (using Bob's account) Hi This is my first itme posting to this group. I've been reading for a little while now. I am a mushroom "hobbyist" if you will. I don't own a microscope or know one reagent from another. Just like to look at, photograph, and occasionally eat the fleshy fungi. The above response caught my eye. What is "Mushroom Magazine"? Where and by whom is it published? Where can I get a sample copy? Any info would be appreciated. Thanx, Mike Smith msmith@discover.wright.edu From owner-mycology@net.bio.net Sat Apr 09 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!agate!howland.reston.ans.net!europa.eng.gtefsd.com!fs7.ece.cmu.edu!cantaloupe.srv.cs.cmu.edu!rnlucas From: rnlucas+@cs.cmu.edu (Bob Lucas) Subject: Re: Portobello Mushrooms Message-ID: Sender: rnlucas@j.gp.cs.cmu.edu Nntp-Posting-Host: j.gp.cs.cmu.edu Organization: School of Computer Science, Carnegie Mellon References: Date: Sun, 10 Apr 1994 15:51:08 GMT Lines: 15 In article , Ed Cottrill wrote: > > Last week I bought some of these at a supermarket. I can't find " >portobello" in any of my refernce books. Could anyone tell me the >scientific name of this fungus? Is it a type of agaricus mushroom? > I believe this is the grown-up version of agaricus bisporus. We also had trouble finding it; Mushroom Magazine did a little article on it. I haven't checked any spore basidia to see if it is actually "bi". Elizabeth Barrow (using Bob's account) From owner-mycology@net.bio.net Sat Apr 09 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!noc.near.net!news.delphi.com!usenet From: Rene Thirion Newsgroups: bionet.mycology Subject: NEED MOLD(?) ID'd Date: Sun, 10 Apr 94 11:54:38 -0500 Organization: Delphi (info@delphi.com email, 800-695-4005 voice) Lines: 29 Message-ID: NNTP-Posting-Host: delphi.com My mothers home has been invaded by a strange and as yet unidentifiable mold (we think) spores of some kind. My brother, who was a landscaper some ten years ago, had a load of mulch - ground up tree bark, etc dumped on my mother's driveway for disemination to various job sites. The mulch had been "imported" from southern Virginia we believe. It was excessively damp. The mulch sat on her driveway for ten days. As family members walked around it they would later find their legs stinging. They removed what appear to be ground pepper-like "things" from their skin. The "things" were black most often, but at some stage of their development they are a lighter color. My family members would often have a difficult time removing them from their skin. But a damp paper towel would remove them. They seem to move toward sources of moisture. They seem to be electrostatically - charged. Family members have placed them in closed jars. Nothing happened. Then they add a damp cotton ball and they begin to multiply dramatically, moving from what seems to be a "black-stage" to white, them possibly a back to a black stage. (I say "possibly" because it is difficult to be sure in non-lab conditions). My mother has been laughed at and ridiculed by so-called health care professionalsMy mother has been laughed at and ridiculed by so-called professionals who think she is imagining these "things". I've seen them, as have other family members. They get into her eyes at night. As I said, they seek out moisture so it can be easily imagined the problems they cause my 75 year old mother. She has taken them to a biology lab at Univ. of MD, where they said only they appear to be alive, electrically charged, possibly mold spores. Can anyone help? From owner-mycology@net.bio.net Sat Apr 09 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!agate!usenet.ins.cwru.edu!magnus.acs.ohio-state.edu!cis.ohio-state.edu!news.sei.cmu.edu!bb3.andrew.cmu.edu!cantaloupe.srv.cs.cmu.edu!rnlucas From: rnlucas+@cs.cmu.edu (Bob Lucas) Subject: Mushroom magazine Message-ID: Sender: rnlucas@j.gp.cd.cmu.edu Nntp-Posting-Host: j.gp.cs.cmu.edu Organization: School of Computer Science, Carnegie Mellon References: Date: Sun, 10 Apr 1994 17:12:49 GMT Lines: 33 In article , Mike Smith wrote: >Bob Lucas (rnlucas+@cs.cmu.edu) wrote: > [previous discussion removed] ... I am a mushroom "hobbyist" if you will. I don't own >a microscope or know one reagent from another. Just like to look at, >photograph, and occasionally eat the fleshy fungi. The above response >caught my eye. What is "Mushroom Magazine"? Where and by whom is it >published? Where can I get a sample copy? Any info would be >appreciated. > Microscopes (and reagents) are often very useful when you're trying to identify a mushroom, although spore prints and a magnifying glass are often enough. For information on subscribing to "Mushroom" (Mushroom the Journal, actually) write: Mushroom Box 3156 University Station Moscow, Idaho 83843 Sorry, I couldn't find a phone number. They tend to concentrate macroscopic fungi, so you might find it more useful than some of the more technical mycological publications. - Elizabeth Barrow (using Bob's account) From owner-mycology@net.bio.net Sun Apr 10 23:00:00 1994 Path: biosci!biosci!not-for-mail From: Peter.Werner@lambada.oit.unc.edu (Peter Gordon Werner) Newsgroups: bionet.agroforestry,bionet.announce,bionet.biology.tropical,bionet.general,bionet.mycology,bionet.plants Subject: RFD: sci.ethnobiology Followup-To: news.groups Date: 11 Apr 1994 15:57:20 -0700 Organization: University of North Carolina Extended Bulletin Board Service Lines: 105 Sender: kristoff@net.bio.net Approved: bionews-moderator@net.bio.net Distribution: world Message-ID: <2o2p9i$t5o@samba.oit.unc.edu> Reply-To: boe666@u.washington.edu (Peter Werner) NNTP-Posting-Host: net.bio.net Xref: biosci bionet.agroforestry:624 bionet.announce:1066 bionet.biology.tropical:389 bionet.general:8578 bionet.mycology:442 bionet.plants:2985 NAME: sci.ethnobiology STATUS: unmoderated PROPONENT: Peter Werner (boe666@u.washington.edu) RATIONALE: There is presently no Usenet newsgroup for the discussion of ethnobiology. Ethnobiological topics are scattered among various newsgroups and mailing lists. Because of this, much of the cross-disciplinary discussion necessitated by the very nature of ethnobiology doesn't take place. This newsgroup would create a place specifically for the discussion of ethnobiological topics. CHARTER: sci.ethnobiology is a newsgroup for the discussion of ethnobiology and its many subfields including ethnobotany, ethnomycology, ethnozoology, ethnomedicine, ethnopharmacology, ethnopsychiatry, ethnoecology, and ethnotaxonomy. Related topics in economic botany, pharmacognosy and pharmacology of naturally-occurring substances, and human ecology are also welcome. An interdisciplinary approach to the ethnobiological questions is encouraged. What this newsgroup isn't: Ethnobiology is sometimes confused with biological anthropology. Biological anthropology is, in fact, a synonym for physical anthropology. Many topics in physical anthropology, such as the biological aspects of human ecology, are relevant to sci.ethnobiology. However, discussion of physical anthropology per se belongs on sci.anthropology. This newsgroup is not alt.drugs.natural. While scientific discussion of the use of psychoactive plants in various cultures and their pharmacognosy and pharmacology is both relevant and welcome, "How do I get high from this" and "Where can I get some of that" threads are not appropriate to this newsgroup. These belong on alt.drugs. Discussion of drug legalization belongs on talk.politics.drugs unless there's a particularly ethnobiological angle of this issue being discussed. "What herbs can I use to cure my headaches", etc. aren't appropriate here either. Discussion of herbs that can cure headaches, etc. is certainly relevant to this newsgroup, but threads specifically looking just for home remedies belong on alt.folklore.herbs. If one can derive some personal health benefit from something discussed on sci.ethnobiology, that's great, but its not the focus of this newsgroup. Ethnobiological questions have a political side and these topics are appropriate to this newsgroup. However, most discussion of environmental politics belongs on talk.environment and alt.save-the-earth. The kind of blind cross-posting that takes place from these groups to sci.environment should not take place here. CROSSPOSTING: This RFD is crossposted in the following groups: alt.drugs news.announce.newgroups alt.folklore.herbs news.groups bionet.agroforestry rec.food.historic bionet.announce sci.anthropology bionet.biology.tropical sci.bio bionet.general sci.bio.ecology bionet.mycology sci.med bionet.plants Several of these groups are gated on to mailing lists: Newsgroup List bionet.agroforestry AGROFORESTRY bionet.announce BIONEWS bionet.biology.tropical TROPICAL-BIOLOGY bionet.general BIOFORUM bionet.mycology MYCOLOGY bionet.plants PLANT-BIOLOGY sci.bio.ecology ECOLOG -L This RFD has also been sent to these mailing lists: Indigenous Knowledge List (INDKNOW%UWAVM.BITNET) Myconet Medlist (medlist@myconet.org) What is the Process of Creating a Newsgroup ----------------------------------------------------- >(a) RFD: Discussion, i.e., public hearing to take place in the newsgroup news.groups for 21-30 days (b) CFV: Call for votes (the voting period will be between 22-30 days) (c) Counting of votes and public display of votes (d) Announcement of new newsgroup (a)-->(b) assumes no major disagreements about this newsgroup during discussion. (c)-->(d) assumes that the vote is favorable, i.e., Y> N+100 and Y> (2/3)(Y+N). Y being the number of YES votes, N being the number of NO votes for the creation of the proposed newsgroup. ------------------------------------------------------------------------------ Peter Werner 4/7/1993 boe666@u.washington.edu -- ------------------------------------------------------------------------------ \ The above does not represent OIT, UNC-CH, laUNChpad, or its other users. / ------------------------------------------------------------------------ From owner-mycology@net.bio.net Mon Apr 11 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!europa.eng.gtefsd.com!news.msfc.nasa.gov!cs.utk.edu!stc06r.CTD.ORNL.GOV!fnnews.fnal.gov!overload.lbl.gov!dog.ee.lbl.gov!hellgate.utah.edu!fcom.cc.utah.edu!u.cc.utah.edu!cwis.isu.edu!cwis.isu.edu!not-for-mail From: rudeeric@cwis.isu.edu (Eric Rude) Newsgroups: bionet.mycology Subject: Ecuador info needed Date: 12 Apr 1994 06:37:19 -0600 Organization: Idaho State University, Pocatello Lines: 15 Distribution: na Message-ID: <2oe4lv$1vo@cwis.isu.edu> NNTP-Posting-Host: localhost.isu.edu Hello I am a biology teacher in Southeastern Idaho. The honors biology class at my school is doing a research project on the rainforests of Ecuador (specifically the Jatun Sacha Biological Station). they have heard that there is a huge variety of fungi there, but have not been able to get any details. Is there anyone out there who might be able to provide them with names of fungi, descriptions, etc., or who might help us find such information? Thanks in advance! -- Eric Rude Pocatello High School 325 N. Arthur, Pocatello, ID 83204-3101 rudeeric@cwis.isu.edu (208)-233-2056 From owner-mycology@net.bio.net Mon Apr 11 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!news.intercon.com!udel!news2.sprintlink.net!news.sprintlink.net!indirect.com!nike From: nike@indirect.com (Laurence Canter) Newsgroups: bionet.mycology,bt.generic.products Subject: Green Card Lottery- Final One? Date: 12 Apr 1994 07:44:33 GMT Organization: Canter & Siegel Lines: 34 Message-ID: <2odjh1$2di@herald.indirect.com> NNTP-Posting-Host: id1.indirect.com Green Card Lottery 1994 May Be The Last One! THE DEADLINE HAS BEEN ANNOUNCED. The Green Card Lottery is a completely legal program giving away a certain annual allotment of Green Cards to persons born in certain countries. The lottery program was scheduled to continue on a permanent basis. However, recently, Senator Alan J Simpson introduced a bill into the U. S. Congress which could end any future lotteries. THE 1994 LOTTERY IS SCHEDULED TO TAKE PLACE SOON, BUT IT MAY BE THE VERY LAST ONE. PERSONS BORN IN MOST COUNTRIES QUALIFY, MANY FOR FIRST TIME. The only countries NOT qualifying are: Mexico; India; P.R. China; Taiwan, Philippines, North Korea, Canada, United Kingdom (except Northern Ireland), Jamaica, Domican Republic, El Salvador and Vietnam. Lottery registration will take place soon. 55,000 Green Cards will be given to those who register correctly. NO JOB IS REQUIRED. THERE IS A STRICT JUNE DEADLINE. THE TIME TO START IS NOW!! For FREE information via Email, send request to cslaw@indirect.com -- ***************************************************************** Canter & Siegel, Immigration Attorneys 3333 E Camelback Road, Ste 250, Phoenix AZ 85018 USA cslaw@indirect.com telephone (602)661-3911 Fax (602) 451-7617 From owner-mycology@net.bio.net Tue Apr 12 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!pipex!zaphod.crihan.fr!jussieu.fr!univ-lyon1.fr!swidir.switch.ch!news.unige.ch!ugun2a!burdetj From: burdetj@uni2a.unige.ch Subject: Adress needed Message-ID: <1994Apr13.180742.1@ugun2a> Lines: 10 Sender: usenet@news.unige.ch Organization: University of Geneva, Switzerland Date: Wed, 13 Apr 1994 16:07:42 GMT Hello. I'm growing mushrooms as a hobby. Where can I order mushrooms' related stuff (books, spores, agar, ...) in europe. ------ Thanks in advance. JF From owner-mycology@net.bio.net Tue Apr 12 23:00:00 1994 Path: biosci!bcm!news.msfc.nasa.gov!europa.eng.gtefsd.com!howland.reston.ans.net!wupost!ukma!seqanal.mi.uky.edu!staben From: staben@seqanal.mi.uky.edu (Chuck Staben) Newsgroups: bionet.mycology Subject: Neursopora protein labelling Date: 13 Apr 1994 00:09:28 GMT Organization: University of Kentucky, Dept. of Math Sciences Lines: 3 Message-ID: <2ofd7o$3fb@s.ms.uky.edu> NNTP-Posting-Host: seqanal.mi.uky.edu X-Newsreader: TIN [version 1.1 PL9] I would like to radioactively label proteins from protoperithecia. Can anyone suggest a protocol that is likely to get them radioactive without getting my whole lab radioactive. From owner-mycology@net.bio.net Tue Apr 12 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.cac.psu.edu!psuvm!djr4 Organization: Penn State University Date: Wed, 13 Apr 1994 15:41:35 EDT From: Mushroom Research Lab Message-ID: <94103.154136DJR4@psuvm.psu.edu> Newsgroups: bionet.mycology Subject: Almost Morel time! Lines: 6 Yes folks, it's nearly that time of the year again. Those morels (black) should be a poppin' anyday now. We're expecting to see them here in central PA around the 18th. If it stays wet and chilly, it'll be a banner year! If anyone sees any.......please let us know. Of course, we'd appreciate the knowledge of the exact location, as well, for research purposes, of course! -------Britt at Penn State From owner-mycology@net.bio.net Tue Apr 12 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.cac.psu.edu!psuvm!djr4 Organization: Penn State University Date: Wed, 13 Apr 1994 15:39:16 EDT From: Mushroom Research Lab Message-ID: <94103.153916DJR4@psuvm.psu.edu> Newsgroups: bionet.mycology Subject: Portobello mushrooms Lines: 2 To anyone concerned: Yes Portobello mushrooms are just big, grown-up Agaricus bisporus!-------Britt From owner-mycology@net.bio.net Wed Apr 13 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!pipex!sunic!trane.uninett.no!daresbury!not-for-mail From: Newsgroups: bionet.mycology Subject: cancelation of subscription Date: 14 Apr 1994 10:28:46 +0100 Lines: 3 Sender: daemon@mserv1.dl.ac.uk Distribution: bionet Message-ID: <2oj2ce$g7u@mserv1.dl.ac.uk> Original-To: mycology@dl.ac.uk I would like to cancel my name from the list of mycology mail newsgroup. Thanks in advance. Pino Macino. From owner-mycology@net.bio.net Wed Apr 13 23:00:00 1994 Path: biosci!BULL.CC.USM.EDU!GLEN_SHEARER From: GLEN_SHEARER@BULL.CC.USM.EDU (GLEN SHEARER) Newsgroups: bionet.mycology Subject: Sacc senescence Date: 14 Apr 1994 06:18:21 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <940414.08182116.064245@USM.CP6> NNTP-Posting-Host: net.bio.net According to Mike Jazqinski's talk at the Southeastern Regional Yeast meeting in Hattiesburg, MS this past month.......it seems that a mother cell can make only a certain number of daughters (the number depends on the strain) before it dies. He's found several genes that influence this "cellular aging". Glen Shearer From owner-mycology@net.bio.net Wed Apr 13 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!vixen.cso.uiuc.edu!news.uoregon.edu!cs.uoregon.edu!usenet.ee.pdx.edu!news.reed.edu!usenet From: tkim@reed.edu (Tae Hoon Kim) Newsgroups: bionet.mycology Subject: senescence in yeast? Date: 14 Apr 1994 01:22:57 GMT Organization: Reed College, Portland, Oregon Lines: 9 Message-ID: <2oi5th$288@scratchy.reed.edu> NNTP-Posting-Host: reed.edu Is it true that in S cerevisiae, the mother cell can only undergo a certain number of budding before degeneration? I would appreciate any comments! Regards, Tae Hoon Kim tkim@reed.edu From owner-mycology@net.bio.net Wed Apr 13 23:00:00 1994 Path: biosci!BULL.CC.USM.EDU!GLEN_SHEARER From: GLEN_SHEARER@BULL.CC.USM.EDU (GLEN SHEARER) Newsgroups: bionet.mycology Subject: Southeastern Yeast Mtg. Date: 14 Apr 1994 15:45:08 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <940414.17461800.065266@USM.CP6> NNTP-Posting-Host: net.bio.net RE: Doug Rhoads The Southeastern Regional Yeast meeting is an annual event recently started in Hattiesburg. It is mostly Saccharomyces people but some other folks as well (My work is on Histoplasma mold/yeast dimorphism). We tried to send invitations to all yeast people we knew of.....sorry we missed you. I'll give your address to the planners for next year's meeting. Glen Shearer From owner-mycology@net.bio.net Wed Apr 13 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!swrinde!gatech!udel!news2.sprintlink.net!news.sprintlink.net!qns1.qns.com!constellation!news.uoknor.edu!ns1.nodak.edu!netnews.nwnet.net!news.u.washington.edu!microb3.biostat.washington.edu!user From: quinnt@u.washington.edu (Matthew Stoecker) Newsgroups: bionet.mycology Subject: RE: Radioactive protein labelling Followup-To: bionet.mycology Date: Wed, 13 Apr 1994 23:04:45 -0800 Organization: University of Washington Lines: 6 Message-ID: NNTP-Posting-Host: microb3.biostat.washington.edu Is there a problem with just incubating with S-35 Methionine in the medium? Although it is somewhat volatile, it's nothing that careful lab technique can't handle. Matthew Stoecker, grad. student U. Wash. Dept. of Microbiology From owner-mycology@net.bio.net Wed Apr 13 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!news.uoregon.edu!netnews.nwnet.net!news.u.washington.edu!boe666 From: boe666@u.washington.edu (Peter G Werner) Newsgroups: bionet.agroforestry,bionet.biology.tropical,bionet.general,bionet.plants,bionet.mycology Subject: Re: RFD: sci.ethnobiology Date: 14 Apr 1994 19:47:10 GMT Organization: University of Washington Lines: 11 Message-ID: <2ok6ju$3it@news.u.washington.edu> References: <2o2p9i$t5o@samba.oit.unc.edu> NNTP-Posting-Host: carson.u.washington.edu Originator: boe666@carson.u.washington.edu Xref: biosci bionet.agroforestry:631 bionet.biology.tropical:392 bionet.general:8616 bionet.plants:3001 bionet.mycology:453 Because of an error that came about in crossposting and because of the delays it caused, I'm temporarily withdrawing this RFD. Its almost summer and Internet traffic will go down a great deal, so proposing a newsgroup at this time of year would be ill-advised. I will put forward this RFD again in September. In the meantime, I'd like to encourage discussion of a sci.ethnobiology newsgroup; the feedback is welcome. Peter Werner boe666@u.washington.edu From owner-mycology@net.bio.net Wed Apr 13 23:00:00 1994 Path: biosci!CMSA.BERKELEY.EDU!EDWARDS%HARBOR1.BITNET From: EDWARDS%HARBOR1.BITNET@CMSA.BERKELEY.EDU Newsgroups: bionet.mycology Subject: Subscription cancellation Date: 14 Apr 1994 08:37:01 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <01HB5PV88G1E000AGK@HARBOR1.BITNET> NNTP-Posting-Host: net.bio.net I would like to cancel my name from the list of the mycology E-mail newsgroup. Thank you in advance. John E. Edwards From owner-mycology@net.bio.net Wed Apr 13 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!bcm!cs.utexas.edu!uunet!emba-news.uvm.edu!moose.uvm.edu!rullrich From: rullrich@moose.uvm.edu (robert ullrich) Subject: Postdoctoral openings in molecular genetics, fungi. Message-ID: <1994Apr14.183117.16578@emba.uvm.edu> Sender: news@emba.uvm.edu Organization: University of Vermont -- Division of EMBA Computer Facility Date: Thu, 14 Apr 1994 18:31:17 GMT Lines: 29 TWO POSTDOCOTRAL POSITIONS IN MOLECULAR GENETICS OF FUNGI. Two post-doctoral fellowships are available in the laboratories of Dr. Robert Ullrich (Department of Botany) and Dr. Charles Novotny (Department of Microbiology and Molecular Genetics) to work as part of their collaborative team studying mating-type regulation of development in the basidiomycete fungus, Schizophyllum commune. The successful candidates will prosecute either of two projects depending upon experience and interest. Project 1 examines the protein complexes of the A Y and Z homeodomain proteins and how these putative transcription factors attain active protein conformations in allelic combinations which activate sexual development (see Proc. Natl. Acad. Sci. USA, 1992, 89:7169-7173 and 7174-7178). Project 2 characterizes genes of the recently isolated A-beta, B-alpha and B-beta loci; these loci will be subjected to structural analysis as described for A- alpha (see references above). Preliminary data show that the A- beta locus also encodes homeodomain proteins and the B-alpha locus encodes a pheromone/pheromone receptor system (i.e., an oligopeptide pheromone and a seven transmembrane domain, serpentine-type receptor). Applicants must have experience in nucleic acids, proteins or antibodies. Successful candidates may also compete for Lucille P. Markey Post-doctoral Fellowships within the Department of Microbiology and Molecular Genetics. To apply send curriculum vitae and three letters of recommendation to: Dr. Robert C. Ullrich, Department of Botany, Life Science Building, University of Vermont, Burlington, VT 05405-0086. The University of Vermont is an AA/EO employer. From owner-mycology@net.bio.net Thu Apr 14 23:00:00 1994 Newsgroups: bionet.agroforestry,bionet.biology.tropical,bionet.general,bionet.plants,bionet.mycology Path: biosci!agate!msuinfo!uwm.edu!post.its.mcw.edu!news.doit.wisc.edu!saimiri.primate.wisc.edu!news.crd.ge.com!sarah!uacsc2.albany.edu!SYSAM From: SYSAM@uacsc2.albany.edu (Aaron Morris) Subject: Can't see the forest for the lack of trees! Message-ID: <16F996A4FS86.SYSAM@uacsc2.albany.edu> Sender: news@sarah.albany.edu (News Administrator) Nntp-Posting-Host: uacsc2.albany.edu Organization: University at Albany, Albany NY 12222 References: <2o2p9i$t5o@samba.oit.unc.edu> <2ok6ju$3it@news.u.washington.edu> Date: Fri, 15 Apr 94 07:33:15 EDT Lines: 34 Xref: biosci bionet.agroforestry:632 bionet.biology.tropical:393 bionet.general:8626 bionet.plants:3006 bionet.mycology:458 Round Lake is a small village in upstate New York that was founded in 1868 as a Methodist campground. It grew into a year round community and is currently listed on the National Register of Historic Places, to recognize the largest collection of Victorian houses in such a concentrated area and also to recognize the oldest, largest, working pipe organ of it's kind in the country. This quaint little village was built under the canopy of a then untouched forest. Unfortunately, the natural balance has been disrupted since the village was built, and new trees have not grown up to replace their parents. Over a century later, a good number of the mature trees are reaching the end of their life cycle and ocasionally a tree will be blown down in high winds or strong storms. This loss of mature trees has been exacerbated by a zealous village superintendent whose attitude is that it's better to chop a tree down before it's time than to have it fall in a storm. The Village has started an agressive tree replacement program, but the old trees are coming down faster than the new trees will grow to fill in the holes in the canopy. I am looking for sources that I can use to back up statements that it is important to move slowly in removing the mature trees from the Village grounds. Arguements along the lines that the mature trees are still valuable to provide housing for fauna are helpful, but weak when stacked up against an irate resident who just had an ancient oak crash down on his car. I can make the case for the value of the mature trees, but I have a hard time defending against lawers seeking hefty settlements should someone be hurt by falling limbs. So, what I'm looking for are arguements that I can use when trying to persuade the Village Superintendent to spare the old trees (he has a $7000 yearly budget item to chop trees and he has been superintendent for almost 20 years) and I need published support for the arguements that I may use. Please help! It's getting to the point that I can't see the forest for the lack of trees! From owner-mycology@net.bio.net Thu Apr 14 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!agate!dog.ee.lbl.gov!ihnp4.ucsd.edu!munnari.oz.au!metro!usage!aix00.csd.unsw.oz.au!p8443882 From: p8443882@aix00.csd.unsw.oz.au (David Orlovich) Subject: Re: mycorrhizae Message-ID: <1994Apr6.041143.5508@usage.csd.unsw.OZ.AU> Sender: news@usage.csd.unsw.OZ.AU Nntp-Posting-Host: aix00.csd.unsw.oz.au Organization: University of New South Wales, Sydney Australia. References: Date: Wed, 6 Apr 1994 04:11:43 GMT Lines: 9 I'd be interested in a mycorrhiza newsgroup too. David Orlovich, School of Botany, University of Melbourne, Parkville, Vic. 3052. From owner-mycology@net.bio.net Thu Apr 14 23:00:00 1994 Path: biosci!IASTATE.EDU!cbronson From: cbronson@IASTATE.EDU (Charlotte R Bronson) Newsgroups: bionet.mycology Subject: Subscription cancellation Date: 15 Apr 1994 07:29:58 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 4 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <9404151430.AA02166@ag2.iastate.edu> NNTP-Posting-Host: net.bio.net Please cancel my name from the list of the mycology E-mail group. If whoever is reading this can't do it, could you please tell me how to cancel? This is my fourth try. C. R. Bronson From owner-mycology@net.bio.net Thu Apr 14 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!swrinde!emory!nigel.msen.com!zib-berlin.de!tertius.in-berlin.de!novell-russo.RZ-Berlin.MPG.DE!bruchez Newsgroups: bionet.mycology Subject: Please stop mailing this group to me!! Message-ID: From: bruchez@MPIMG-Berlin-Dahlem.MPG.DE (Jon J. P. Bruchez) Date: Fri, 15 Apr 1994 19:38:29 UNDEFINED Organization: Max-Planck-Institut für Molekulare Genetik, Berlin. Nntp-Posting-Host: novell-russo.rz-berlin.mpg.de Nntp-Posting-User: nntp_client X-Newsreader: Trumpet for Windows [Version 1.0 Rev B] Lines: 25 To whom it may concern, I was clearly added to the direct-mailing list for this group (bionet.mycology) a year or so ago. Which was fine for a while but as my e-mail address has changed since then the postings find themselves wandering around the country with nowhere to go and causing my network controller no end of headaches. I still enjoy reading articles posted within this group but as I now use a speceific news reader package mailing me direct is no longer required. I've looked through the network but cannot find how to access the list where my name nad e-mail address is located or find any information on how to remove my name form that list. I hope that somebody responsible for that list is reading this and deal with this for me. Thanks in advance to anyone who can help. Please mail me at my correct address given below to let me now if this problem has been dealt with. Jon. -------------------------------------------------------- Jon J. P. Bruchez. Max-Planck Institut für molekulare Genetik, Ihnestraße 73, 14195 Berlin, Germany e-mail: Bruchez@MPIMG-Berlin-Dahlem.MPG.DE From owner-mycology@net.bio.net Fri Apr 15 23:00:00 1994 Path: biosci!agate!darkstar.UCSC.EDU!arapaho!nathan From: nathan@cse.ucsc.edu (Nathan J. Wilson) Newsgroups: bionet.mycology Subject: Taxy 0.0 is available now! Date: 15 Apr 1994 19:05:01 GMT Organization: UC Santa Cruz CIS/CE Lines: 96 Message-ID: <2omogt$k7e@darkstar.UCSC.EDU> NNTP-Posting-Host: arapaho.cse.ucsc.edu X-Newsreader: TIN [version 1.2 PL2] Well, I have finally got my taxonomic database system to the state that it is ready for its first public release. I've included a copy of the README file below that includes the information needed to download a copy of it. Enjoy! -Nathan ------ Welcome to Taxy 0.0! Taxy is a database system for helping with biological taxonomic identification. For those of you which didn't understand the last sentence think of it as a computerized field guide. The goal of the current version is to get people working on creating a database of mushroom species. All work done with the system is expected to remain freely available in keeping with the goal of the project to create a system for sharing fungal information freely and to permit easy database expansion and interchange by interested users. The project is part of my computer science masters thesis. The 0.0 release includes: - A Taxy database emphasizing macroscopic characteristics. This database currently has 170 features and contains partial information for about 50 species of fungi. - Support for creating, browsing and editing Taxy databases. Taxy is designed to support any type of taxonomic information so, for example, it should be possible for an interested person to create a database of bird or plant descriptions. - An on-line help system. This is the only form of documentation currently available. - Executables for the Apple Macintosh, Sun SPARC, and IBM PCs. (Source code is available upon request, though I would prefer to wait for the next release to distribute source unless you are interested in actually helping to extend the system.) All versions are available for anonymous ftp from the machine: ftp.cse.ucsc.edu in the directory: taxy The mac version is a binhexed, StuffIt archive dist.mac/Taxy.sit.hqx The SPARC version is availble either as a gzipped, tar archive: dist.sun/taxysun.tar.gz Or as the files: dist.sun/taxy dist.sun/deep.db dist.sun/help/* dist.sun/comments/* The IBM version is availble either as a gzipped, tar archive: dist.pc/taxypc.tar.gz Or as the files: dist.pc/taxy dist.pc/deep.db dist.pc/help/* dist.pc/comments/* This release does *not* (but the second release will) include: - A useful searching facility for actually doing identification with the system. This part of the project is still under construction. - Automatic merging functions for combining multiple existing databases. - Features for describing the microscopic aspects of fungi and a more complete treatment of features related to Ascomycetes and Gasteromycetes. (Although the truly ambitious could add these features themselves.) - A general scripting system for creating database specific commands for entering information. If you are interested in participating in any way please drop me a note via email or call me at: (408)423-3773. If you wish to help extend the database, please contact me so I can arrange a set of species for you to work on so effort is not duplicated. ------------------------- _________ Nathan Wilson <_________> nathan@cse.ucsc.edu _|_|_ It is no dream! Co-Science Advisor \___/ Matsutake are growing Minister of Local Forays | | On the belly of the mountain. Fungus Federation of Santa Cruz \_/ *83-- -Shigetaka From owner-mycology@net.bio.net Fri Apr 15 23:00:00 1994 Path: biosci!agate!darkstar.UCSC.EDU!arapaho!nathan From: nathan@cse.ucsc.edu (Nathan J. Wilson) Newsgroups: bionet.mycology Subject: Taxy 0.0 first correction! Date: 15 Apr 1994 20:13:11 GMT Organization: UC Santa Cruz CIS/CE Lines: 21 Message-ID: <2omsgn$lbf@darkstar.UCSC.EDU> NNTP-Posting-Host: arapaho.cse.ucsc.edu X-Newsreader: TIN [version 1.2 PL2] The directory containing the software is: tmp/taxy not just taxy (again the machine is ftp.cse.ucsc.edu) Also I have added a new file 'example' that contains a text example of using the system to enter a new species and then look at the results. By way of warning, the system has not been heavily tested. You should consider it to be an Alpha release at best. Use the appropriate degree of caution. The system gets particularly cranky if it runs out of memory. If you have problems there should be a file left behind called TaxyLog. (If you have more than one problems they will be called TaxyLogA, TaxyLogB ...) Please send any such file when you report a problem. Thanks, -Nathan From owner-mycology@net.bio.net Fri Apr 15 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!agate!howland.reston.ans.net!news.intercon.com!psinntp!psinntp!psinntp!newstand.syr.edu!rodan.syr.edu!jworrall From: jworrall@rodan.syr.edu (James J. Worrall) Subject: Re: NEED MOLD(?) ID'd Message-ID: <1994Apr15.170312.3496@newstand.syr.edu> Sender: netnews@newstand.syr.edu (Network News Administrator) Nntp-Posting-Host: rodan.syr.edu Organization: SUNY Coll. Envtl. Sci. & Forestry, Syracuse, NY References: Date: Fri, 15 Apr 1994 17:03:12 GMT Lines: 6 The fungus is probably Sphaerobolus stellatus. It is a basidiomycete that shoots a spore-containing projectile of the sort you described up to a distance of 15 meters. It usually grows on hardwood mulch and has caused expensive damage to cars and home siding in some situations as the fungal adhesive is extremely efficient. From owner-mycology@net.bio.net Sat Apr 16 23:00:00 1994 Path: biosci!MAGNUS.ACS.OHIO-STATE.EDU!alambowi From: alambowi@MAGNUS.ACS.OHIO-STATE.EDU (Alan M Lambowitz) Newsgroups: bionet.mycology Subject: unsubscribe Date: 17 Apr 1994 16:40:23 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <199404172340.TAA03145@beauty.magnus.acs.ohio-state.edu> NNTP-Posting-Host: net.bio.net Please delete my E-mail subscription. From owner-mycology@net.bio.net Sat Apr 16 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!torn!news.unb.ca!UNBSJ.CA!c0hx From: c0hx@UNBSJ.CA (JASON K. DOBRANIC) Newsgroups: bionet.mycology Subject: Looking for the following plant pathogens Date: 17 Apr 1994 21:15:36 GMT Organization: UNB Saint John Campus Lines: 18 Message-ID: NNTP-Posting-Host: 138.119.1.180 Hello, I want to conduct a study on the potential of a fungal antagonist for the biocontrol of eastern larch diseases and was wondering if anyone has access or can put me in contact with an appropriate place where I might get a culture of one or more of the following pathogens: Hypodermella laricis Armillaria mellea Scytinostroma galactinum Phaeolus schweinitzii Inonotus tomentosus Fomitopsis officinalis Phellinus pini Climacocystis borealis Thank you, Jason From owner-mycology@net.bio.net Sat Apr 16 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!gatech!taco.cc.ncsu.edu!crook From: crook@unity.ncsu.edu (Robert P. Crook) Newsgroups: bionet.mycology Subject: mycorrhizae-nutrient cycling Date: 17 Apr 1994 08:28:09 GMT Organization: North Carolina State University Lines: 9 Message-ID: <2oqrup$q9d@taco.cc.ncsu.edu> NNTP-Posting-Host: cc02du.unity.ncsu.edu X-Newsreader: TIN [version 1.2 PL2] I would be also interested in a mycorrhizae newsgroup; of particular interest would be what role ectomycorrhizae play in the nutrient cycling of pine plantations/forests. Rob Rob Crook Dept. of Forestry North Carolina State Univ. Raleigh, N.C. From owner-mycology@net.bio.net Sat Apr 16 23:00:00 1994 Path: biosci!waite.adelaide.edu.au!pmurphy2 From: pmurphy2@waite.adelaide.edu.au (Phil Murphy) Newsgroups: bionet.mycology Subject: unsubcribe Date: 16 Apr 1994 19:54:46 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <9404170255.AA00262@schooner.waite.adelaide.edu.au> NNTP-Posting-Host: net.bio.net please take me off the network From owner-mycology@net.bio.net Sat Apr 16 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!agate!howland.reston.ans.net!EU.net!uknet!demon!karon.demon.co.uk!user From: gareth@karon.demon.co.uk (Gareth Price) Subject: help needed: mushroom growth Message-ID: Followup-To: bionet.mycology Sender: news@demon.co.uk (Usenet Administration) Nntp-Posting-Host: karon.demon.co.uk Organization: Demon Internet Date: Sun, 17 Apr 1994 17:19:50 GMT Lines: 10 I am a writer producing books for scdhool students. I am looking for data aboiut the growth rate of mushrooms (particularly Agaricus bisporus) in different conditions - ideally linked to commercial production. I am interested in yield wrt temperature, nutrient content of substrate, mushroom type etc. All help gratefully received. Thanks -- gareth@karon.demon.co.uk From owner-mycology@net.bio.net Mon Apr 18 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!pipex!uunet!news.iij.ad.jp!wnoc-tyo-news!sh.wide!wnoc-snd-ss2!sakunami!diptx1!NewsWatcher!user From: AB143@kdw01.kj.yamagata-u.ac.jp (Keitaro Tawaraya) Newsgroups: bionet.mycology Subject: mycorrhiza-arbuscular Followup-To: bionet.mycology Date: Tue, 19 Apr 1994 12:26:23 +0000 Organization: Yamagata University Lines: 8 Message-ID: NNTP-Posting-Host: tawa I would be interested in a mycorrhizae newsgroup; of particular interest would be mechanisms of arbuscular mycorrhizal infection. -- Keitaro Tawaraya Yamagata University E-mail:AB143@kdw01.kj.yamagata-u.ac.jp Phone:0235-23-1521(2402) Fax:0235-24-1801 From owner-mycology@net.bio.net Mon Apr 18 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!daresbury!trane.uninett.no!eunet.no!EU.net!uknet!festival!leeds.ac.uk!news From: ecl6dt@leeds.ac.uk (Doug Tingle) Subject: Book wanted for cash Organization: University of Leeds Date: Tue, 19 Apr 1994 08:37:49 GMT Message-ID: X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Originator: news@gps1 Sender: news@leeds.ac.uk Lines: 2 Has anyone got a copy of Microfungi on Land Plants by Ellis & Ellis with which they are prepared to part for cash? From owner-mycology@net.bio.net Mon Apr 18 23:00:00 1994 Path: biosci!SUMMA.TAMU.EDU!dje0282 From: dje0282@SUMMA.TAMU.EDU (Dan Ebbole) Newsgroups: bionet.mycology Subject: fungal PR Date: 19 Apr 1994 06:48:34 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <199404191348.GAA03059@net.bio.net> NNTP-Posting-Host: net.bio.net There is a nice article on mycology research in the April 18 issue of "The Scientist". ------------------------ Dr. Daniel Ebbole dje0282@summa.tamu.edu Dept. of Plant Pathology and Microbiology Texas A&M University College Station, TX 77843-2132 From owner-mycology@net.bio.net Mon Apr 18 23:00:00 1994 Path: biosci!daresbury!trane.uninett.no!sunic!pipex!howland.reston.ans.net!torn!news.unb.ca!UNBSJ.CA!c0hx From: c0hx@UNBSJ.CA (JASON K. DOBRANIC) Newsgroups: bionet.mycology Subject: Thanks for help. Date: 19 Apr 1994 22:57:40 GMT Organization: UNB Saint John Campus Lines: 3 Message-ID: NNTP-Posting-Host: 138.119.1.180 I want to thank everyone who wrote me about getting the pathogenic cultures I wanted. This net is great! Jason From owner-mycology@net.bio.net Mon Apr 18 23:00:00 1994 Path: biosci!daresbury!not-for-mail From: buxton@fmi.ch (Buxton Frank) Newsgroups: bionet.mycology Subject: Novozyme supplies Date: 19 Apr 1994 11:02:17 +0100 Lines: 16 Sender: daemon@mserv1.dl.ac.uk Distribution: bionet Message-ID: <2p0a79$35q@mserv1.dl.ac.uk> Original-To: mycology@dl.ac.uk Can anyone tell me where they are getting Novozyme for making Aspergillus spheroplasts from. We have in the past obtained it directly from Novo, but now they will only supply 500g or more and from Sigma the price is fairly horrendous. Thanks Frank Buxton email: buxton@fmi.ch From owner-mycology@net.bio.net Tue Apr 19 23:00:00 1994 Path: biosci!daresbury!trane.uninett.no!sunic!EU.net!ub4b!news.sri.ucl.ac.be!NewsWatcher!user From: Moulliard@mbla.ucl.ac.be (Moulliard) Newsgroups: bionet.mycology Subject: Strain deposit Followup-To: bionet.mycology Date: Wed, 20 Apr 1994 09:03:04 +0200 Organization: MUCL Lines: 34 Message-ID: NNTP-Posting-Host: 130.104.82.1 Dear Colleagues, The MUCL culture collection is a public collection working in the field of the biodiversity conservation and biotechnology for the agro-industry. We have developed our expertise in the identification of different group of Fungi and Yeasts (especially in Deuteromycetes, Basidiomycetes and Ascomycetes). Today, to preservate and conserve the biodiversity, we search Fungi or Yeasts strains of interest. Most of the scientists make a deposit only of type strain for publication purposes while numerous other original strains can be of interest for the scientific community. Incorporation of interesting original strains in a public collection can help to make them available to everyone. If you want to deposit some original strains (strains with publication or strain original from particular biotope) in our collection, we will very please to receive them. The MUCL culture collection as an "International Deposit Authority" accept also the deposit of patented strains under the Budapest Treaty. We will very happy if we can collaborate in the future in a fruitful way. Free feel to contact us. Best regards, Yours sincerely Charles Moulliard -- Mycotheque de l'Universite Catholique de Louvain (MUCL) Place Croix du Sud, 3 B-1348 Belgium Tel.: +32-(10)-47.37.37 Fax.:+32-(10)-45.15.01 Email: Moulliard@mbla.ucl.ac.be From owner-mycology@net.bio.net Tue Apr 19 23:00:00 1994 Path: biosci!agate!overload.lbl.gov!dog.ee.lbl.gov!ihnp4.ucsd.edu!sdd.hp.com!vixen.cso.uiuc.edu!news.uoregon.edu!cs.uoregon.edu!usenet.ee.pdx.edu!not-for-mail From: billh@cs.pdx.edu (William J Hunt) Newsgroups: bionet.mycology Subject: Re: Almost Morel time! Date: 19 Apr 1994 16:48:55 -0700 Lines: 20 Message-ID: <2p1ql7$1k6@sirius.cs.pdx.edu> References: <94103.154136DJR4@psuvm.psu.edu> NNTP-Posting-Host: sirius.cs.pdx.edu X-Newsreader: NN version 6.4.19 #2 Mushroom Research Lab writes: >Yes folks, it's nearly that time of the year again. Those morels (black) >should be a poppin' anyday now. We're expecting to see them here in central >PA around the 18th. If it stays wet and chilly, it'll be a banner year! If >anyone sees any.......please let us know. Of course, we'd appreciate the >knowledge of the exact location, as well, for research purposes, of course! > -------Britt at Penn State found a few morels last weekend, wandering around beneath cottonwoods on sandy soil along the Columbia river near Portland Oregon ... looks like the season is just starting; found Verpa bohemica past prime. lots of other foragers out too. -- William Hunt, Portland, Oregon billh@cs.pdx.edu From owner-mycology@net.bio.net Tue Apr 19 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!daresbury!trane.uninett.no!eunet.no!EU.net!uknet!festival!leeds.ac.uk!news From: ecl6dt@leeds.ac.uk (Doug Tingle) Subject: BOOK WANTED Organization: University of Leeds Date: Wed, 20 Apr 1994 08:20:03 GMT Message-ID: X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Sender: news@leeds.ac.uk Lines: 10 If anyone has a copy of "MICROFUNGI ON LAND PLANTS" by Ellis & Ellis with which they are prepared to part for cash, please let me know. Doug Tingle (ecl6dt@leeds.ac.uk) The University of Leeds Computing Service Leeds LS2 9JT tel. 0532 335368 From owner-mycology@net.bio.net Tue Apr 19 23:00:00 1994 Path: biosci!daresbury!trane.uninett.no!eunet.no!EU.net!howland.reston.ans.net!europa.eng.gtefsd.com!news.umbc.edu!haven.umd.edu!purdue!lerc.nasa.gov!magnus.acs.ohio-state.edu!csn!col.hp.com!rcm From: rcm@col.hp.com (Zippy the Pinhead) Newsgroups: bionet.mycology Subject: Re: Taxy 0.0 is available now! Date: 20 Apr 1994 19:38:33 GMT Organization: Hewlett-Packard, Colo. Spgs., CO Lines: 15 Message-ID: <2p40bp$5t2@hp-col.col.hp.com> References: <2omogt$k7e@darkstar.UCSC.EDU> NNTP-Posting-Host: hpctdfc.col.hp.com X-Newsreader: TIN [version 1.2 PL0.7] Nathan J. Wilson (nathan@cse.ucsc.edu) wrote: : Well, I have finally got my taxonomic database system to the state that : it is ready for its first public release. I've included a copy of the : README file below that includes the information needed to download a : copy of it. : All versions are available for anonymous ftp from the machine: : ftp.cse.ucsc.edu : in the directory: : taxy NOT! Try again. Rick From owner-mycology@net.bio.net Tue Apr 19 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!agate!ihnp4.ucsd.edu!swrinde!gatech!europa.eng.gtefsd.com!news.msfc.nasa.gov!cs.utk.edu!stc06r.CTD.ORNL.GOV!ornl!mars!matis From: matis@mars.epm.ornl.gov (Sherri Matis) Subject: Re: Almost Morel time! Message-ID: <1994Apr20.141103.3794@ornl.gov> Sender: usenet@ornl.gov (News poster) Reply-To: matis@mars.epm.ornl.gov Organization: Oak Ridge National Lab/CESAR References: <2p1ql7$1k6@sirius.cs.pdx.edu> Date: Wed, 20 Apr 1994 14:11:03 GMT Lines: 20 Sorry guys but I can't resist gloating. After a few years of foraging in western Pa. and finding only enough M. esulenta for 2 or 3 decent meals, I finally hit pay dirt. for the last 2 weekends I have picked over 300, big ones, small ones, even a Verpa. On the last hunt we had stiff competion and we still found over 100. Mostly smaller varieties were found on the first week M. deliciosa (sic), and black morels, some smaller esculenta. The 2nd week the esculenta were much larger, 10-15 cms. I think the season may be about over down here, but it should be starting very soon in Pa. I always found giant ones about the 2nd week in may in Pa. and we are at least a month ahead down here. And, I gave part of the bounty away to friends and family Happy hunting, Sherri From owner-mycology@net.bio.net Wed Apr 20 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!swrinde!gatech!news-feed-2.peachnet.edu!news-feed-1.peachnet.edu!news.duke.edu!eff!cs.umd.edu!news.umbc.edu!haven.umd.edu!purdue!mozo.cc.purdue.edu!inet.d48.lilly.com!mcvax2.d48.lilly.com!rx81238 Newsgroups: bionet.mycology Subject: Re: Novozyme supplies Message-ID: <1994Apr21.171903.1@mcvax2.d48.lilly.com> From: rx81238@mcvax2.d48.lilly.com Date: 21 Apr 94 17:19:03 EST References: <2p0a79$35q@mserv1.dl.ac.uk> Distribution: bionet,world Nntp-Posting-Host: mcvax2.d48.lilly.com Lines: 24 In article <2p0a79$35q@mserv1.dl.ac.uk>, buxton@fmi.ch (Buxton Frank) writes: > > > > Can anyone tell me where they are getting Novozyme for making > Aspergillus spheroplasts from. We have in the past obtained it > directly from Novo, but now they will only supply 500g or more > and from Sigma the price is fairly horrendous. > > Thanks > > Frank Buxton > email: buxton@fmi.ch > We get it from Calbiochem, but I don't know the price offhand nor the relative expense compared to Sigma. Jeff Radding jar@lilly.com Opinions expressed are my own and do not represent those of Eli Lilly and Co. > > > From owner-mycology@net.bio.net Wed Apr 20 23:00:00 1994 Path: biosci!agate!darkstar.UCSC.EDU!arapaho!nathan From: nathan@cse.ucsc.edu (Nathan J. Wilson) Newsgroups: bionet.mycology Subject: Taxy 0.01 (bug fix) Date: 20 Apr 1994 22:43:01 GMT Organization: UC Santa Cruz CIS/CE Lines: 55 Message-ID: <2p4b5l$78c@darkstar.UCSC.EDU> NNTP-Posting-Host: arapaho.cse.ucsc.edu X-Newsreader: TIN [version 1.2 PL2] Taxy 0.01 is now available for anonymous ftp from: ftp.cse.ucsc.edu in the directory: tmp/taxy I've reorganized the files a little bit, so if you're confused look at the file: tmp/taxy/README This release is just a bug fix of version 0.0. Here's a list of fixed bugs in both the program and the associated databases. Also a list of known bugs that are still in the system (nothing that should get in anyone's way). Fixed bugs: If you give a multi-word value for the preferred value for Common Name they get entered as separate alternates In add mode you need to add a comma after ? in order to lookup help on a particular subject. Members fields doesn't get set correctly. Don't worry about it for now. Can't leave feature blank in add mode after you request help. Changing the value of an inactive feature does not properly activate the features above it. Float range features always get reset to the range 0-0 Known Bugs: New feature values should go in the right place immediately, rather than waiting for save and reload. This will be fixed in the next major release. Can't have people with the same canonical full name. Fixing this is kind of hairy and will have to wait for the next major release Changes from 0 to 1 of deep.db and features.db: Canalliculate -> Canaliculate Corruate -> Corrugate Glabrose -> Glabrous bifurcate -> Bifurcate ApicalPort -> ApicalPore Gills margin distinctive -> Gill margin distinctive Umbillicate -> Umbilicate GlClOd was a term feature is now an alias feature GlMrCl was a term feature is now an alias feature LtClCh was a term feature is now an alias feature The following typos used to be canonical values so are still supported as typos so databases still load correctly: Canalliculate Glabrose Enjoy! ------------------------- _________ Nathan Wilson <_________> nathan@cse.ucsc.edu _|_|_ It is no dream! Co-Science Advisor \___/ Matsutake are growing Minister of Local Forays | | On the belly of the mountain. Fungus Federation of Santa Cruz \_/ *83-- -Shigetaka From owner-mycology@net.bio.net Wed Apr 20 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.cac.psu.edu!psuvm!djr4 Organization: Penn State University Date: Thu, 21 Apr 1994 17:30:08 EDT From: Mushroom Research Lab Message-ID: <94111.173008DJR4@psuvm.psu.edu> Newsgroups: bionet.mycology Subject: the elusive morel Lines: 2 Anyone having luck finding morels? So far I've only heard a few successes..... .....they don't seem to be up yet in Central PA. Happy hunting, BB From owner-mycology@net.bio.net Wed Apr 20 23:00:00 1994 Path: biosci!VENUS.TAMU.EDU!npk3325 From: npk3325@VENUS.TAMU.EDU (Nancy Keller) Newsgroups: bionet.mycology Subject: S.rolfsii mutagenesis Date: 21 Apr 1994 06:38:08 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <199404211338.GAA27659@net.bio.net> NNTP-Posting-Host: net.bio.net I'm looking for suggestions on best way to do mutagenesis w/ S. rolfsii. > This is a multinucleate, prob. heterothallic basidiomycete which produces > no spores (of a useful quantity). So, I thought to irradiate either > mycelial fragments or protoplasts w/ UV. Goal is to create some useful > markers. Any suggestions or experience w/ this fungus or similar ones? > Thanks, Nancy Keller, Texas A&M Univ., Plant Patholo & Microbiol From owner-mycology@net.bio.net Thu Apr 21 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!agate!howland.reston.ans.net!pipex!uknet!festival!leeds.ac.uk!news From: ecl6dt@leeds.ac.uk (Doug Tingle) Subject: BOOK WANTED Organization: University of Leeds Date: Fri, 22 Apr 1994 08:22:14 GMT Message-ID: X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Sender: news@leeds.ac.uk Lines: 9 If anyone has a copy of "MICROFUNGI on LAND PLANTS" by Ellis & Ellis with which they are prepared to part for cash, please let me know. D. Tingle The University of Leeds Computing Service Leeds LS2 9JT tel 0532 335368 From owner-mycology@net.bio.net Thu Apr 21 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!EU.net!ub4b!news.sri.ucl.ac.be!NewsWatcher!user From: Moulliard@mbla.ucl.ac.be (Moulliard) Newsgroups: bionet.mycology Subject: WORKSHOP & MUCL CENTENARY Followup-To: bionet.mycology Date: Fri, 22 Apr 1994 08:11:30 +0200 Organization: MUCL Lines: 212 Message-ID: NNTP-Posting-Host: 130.104.82.2 ************************************************* JUNE 29, 1994 LOUVAIN-LA-NEUVE, BELGIUM 1894-1994 MYCOTHEQUE DE L'UNIVERSITE CATHOLIQUE DE LOUVAIN MUCL CENTENARY "FUNGAL TAXONOMY AND TROPICAL MYCOLOGY: QUO VADIS ?" ************************************************* INVITATION Professor P. Macq, Rector of the UniversitŽ Catholique de Louvain, the UCL Mycothque, the UCL Laboratory of Systematic and Applied Mycology and the Coordinator of the Belgian Coordinated Collections of Microorganisms (BCCM) have the pleasure of inviting you to celebrate, the 100th Anniversary of MUCL on Wednesday, June 29th, 1994. On this occasion, an international workshop will be held on "FUNGAL TAXONOMY AND TROPICAL MYCOLOGY: QUO VADIS ?" Modern taxonomy, including mycotaxonomy, relies in an increasing degree on new molecular approaches. As such, one can observe that the traditional morphotaxonomic approach is more and more complemented - or sometimes even substituted - by chemotaxonomic methods or genome-based analyses. Questioning the (added) value and mutual complementarity of all available tools - both classical and modern - is essential to be able to handle the systematic classification problem in an integrated and effective way. On the other hand, the greater part of the fungal biodiversity has still to be discovered and described especially in the tropical regions. As these are mainly situated in developing countries, one has to discuss - in the spirit of the Rio de Janeiro earth summit - key topics such as training, technology transfer and the set-up of local 'ex situ' collections. During such an exercise, special attention has to be given to taxonomic and preservation methods that are not only effective but also widely applicable, user-friendly and robust. ************************************************* PROGRAMME June 29th, 1994 1894-1994 MUCL Centenary 9:00 to 12:00 am: Centenary celebration H. Naveau, UCL Opening lecture. P. Macq, Rector, UCL G.L. Hennebert, UCL Mycothque de l'UniversitŽ Catholique de Louvain (MUCL): 100 Years of existence and activity of the fungus culture collection. Guest speakers: D. Hawksworth, IMI, U.K. Fungal genetic resource collections and biodiversity. D. van der Mei, CBS, MINE, Netherlands The fungus culture collections in Europe. J. De Brabandere, BCCM, Belgium The Belgian Coordinated Collections of Microorganisms. 12:00 to 1:30 pm: Buffet of Belgian specialties 1:30 to 6:00 pm: Workshop (provisional titles) Introduction: G.L. Hennebert, UCL Fungal taxonomy and tropical mycology: Quo vadis ? Guest speakers: C.P. Kurtzman, NRRL, USA Molecular taxonomy in the yeast fungi: present and future. M. Blackwell, Louisiana State University, USA Phylogeny of filamentous fungi deduced from analysis of molecular characters: present and future. J. Rammeloo, National Botanical Garden, Belgium Importance of morphological and anatomical characters in fungal taxonomy. M.F. Roquebert, Natural History Museum, France Possible progress of modern morphological analysis in fungal taxonomy. A.J. Masuka, Forest Research Center, Zimbabwe Mycofloristic studies in South Central Africa: status, constraints, opportunities and strategies. A. Peerally, University of Mauritius, Mauritius Strategies for the development of tropical mycology. E.J. Da Silva, MIRCEN, UNESCO, France The role of the Microbiological Resources Centers (MIRCEN) in the development of mycology in the tropics. Discussion and conclusions. Evening: Anniversary Banquet ************************************************* CALL FOR POSTERS Topics concerning the workshop are welcome. Authors are kindly requested to send the title and an abstract of their poster before May 1st, 1994. ************************************************* FEES Registration for Centenary celebration and workshop are without charge. Booking for the accommodation and banquet has to be paid. The fee for the Anniversary banquet is 1.800 BEF (US$ 50). ************************************************* TRANSPORTATION By air All airlines provide air service to the International Brussels Airport in Zaventem, about 30 km from Louvain-la-Neuve. A shuttle between the Airport and Louvain-la-Neuve will be organized on Tuesday, June 28th, 1994 for participants. By train Inter-City trains operate every 30 min. from Brussels North, Central and Midi Stations to Ottignies (direction Namur). At Ottignies, there is a train to Louvain-la-Neuve. By road Participants arriving by road should take the E-411 motorway Bruxelles- Namur-Luxembourg and exit at n¡ 7 (coming from Brussels) or exit at n¡ 9 (coming from Namur). ************************************************ SOCIAL EVENTS On Wednesday afternoon, June 29th, 1994, you will be able to visit the MUCL Fungus Culture Collection and the exhibit hall. The Anniversary Banquet on Wednesday evening, June 29th, will be held at the Villers-la-Ville Abbey Farm. ************************************************* TOURIST ATTRACTIONS All major Belgian tourist cities (Brussels, Namur, Lige, Bruges, Ghent, ...) are easily accessible by train or car from Louvain-la-Neuve. Information can be obtained at the railway station in Louvain-la-Neuve or at MUCL. Reduced prices are offered for group and/or one-day trips. ************************************************* SCIENTIFIC EXHIBITIONS Parallel to the workshop, scientifics posters will be exposed. Several companies will present laboratory equipments and products in the field of microbiology on Wednesday, June 29th, 1994. ************************************************* ORGANIZING COMMITTEE G.L. Hennebert, J. Decallonne (UCL) J. De Brabandere (SPPS-BCCM) P. Charue, C. Decock, P. Evrard, N. Jamin, P. Massart, C. Moulliard, L. NŽlissen, S. Vanhulle (UCL-MUCL) In collaboration with REUL - Service des Relations ExtŽrieures (UCL) For a registration program CALL, FAX, E-mail or WRITE to Prof. Dr. G.L. Hennebert MUCL 100 Years 1894-1994 Mycothque de l'UniversitŽ Catholique de Louvain 3 Place Croix du Sud B-1348 Louvain-la-Neuve Belgium Tel. + 32-(0)10-473742 Fax: + 32-(0)10-451501 E-Mail: Moulliard@mbla.ucl.ac.be ************************************************* -- Moulliard Charles Mycotheque de l'Universite Catholique de Louvain (MUCL) Place Croix du Sud, 3 B-1348 Belgium Tel.: +32-(10)-47.37.37 Fax.:+32-(10)-45.15.01 Email: Moulliard@mbla.ucl.ac.be From owner-mycology@net.bio.net Thu Apr 21 23:00:00 1994 Path: biosci!SUMMA.TAMU.EDU!dje0282 From: dje0282@SUMMA.TAMU.EDU (Dan Ebbole) Newsgroups: bionet.mycology Subject: Texas fungal researchers-seminar announcement. Date: 22 Apr 1994 10:46:45 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <199404221746.KAA01996@net.bio.net> NNTP-Posting-Host: net.bio.net Dr. David Perkins will give a seminar at 4 pm, Thursday May 5 at Texas A&M. "Meiotic Drive, pseudohomothallism, and introgression in Neurospora" Colleagues in Texas within driving distance - (Houston, Dallas, and Austin) are invited to attend. e-mail for more information. ------------------------ Dr. Daniel Ebbole dje0282@summa.tamu.edu Dept. of Plant Pathology and Microbiology Texas A&M University College Station, TX 77843-2132 From owner-mycology@net.bio.net Thu Apr 21 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.intercon.com!panix!zip.eecs.umich.edu!newsxfer.itd.umich.edu!nntp.cs.ubc.ca!alberta!quartz.ucs.ualberta.ca!tribune.usask.ca!canopus.cc.umanitoba.ca!frist From: frist@cc.umanitoba.ca () Newsgroups: bionet.mycology Subject: Grad. Asst. - MOL. BIO. OF PLANT/FUNGAL INTERACTIONS Date: 22 Apr 1994 20:17:13 GMT Organization: University of Manitoba, Winnipeg, Manitoba, Canada Lines: 53 Distribution: world Message-ID: <2p9bc9$t7u@canopus.cc.umanitoba.ca> NNTP-Posting-Host: vogt.cc.umanitoba.ca **************************************************************************** GRADUATE RESEARCH ASSISTANTSHIP MOLECULAR BIOLOGY OF PLANT/FUNGAL INTERACTIONS **************************************************************************** RESEARCH PROGRAM - Our laboratory is investigating the role of differential expression of members of defense multigene families in plants as part of their resistance response to fungal pathogens. The "pathogenesis-related" gene family drr49 is expressed in many plant species in response to fungi, bacteria and elicitors. We are using the garden pea (Pisum sativum) as a model system to investigate the following questions : 1) Do drr49 multigene family members play specific roles in the defense response? 2) Are specialized roles for drr49 genes conserved among wild relatives of pea? 3) Is differential expression of drr49 genes a consequence of differential binding by transcription factors? 4) Do pea drr49 genes, when transformed into canola (Brassica napus) exhibit induction in response to pea pathogens and/or canola pathogens? QUALIFICATIONS - B.S. or M.Sc. in Genetics, Biochemistry, Plant Pathology or related field. The ideal candidate will have a strong background in Plant Pathology and some experience in recombinant DNA. THE POSITION - Funding for an M.Sc. or Ph.D. student is available September 1994 through March 1997. Applications must be received by June 1, 1994. THE UNIVERSITY, WINNIPEG AND MANITOBA - The University of Manitoba is one of Canada's major centers for agricultural research. Other academic and research institutions in Winnipeg include Agriculture Canada, the U. of M. Health Sciences campus and the Univ. of Winnipeg. Winnipeg offers a cosmopolitan mixture of culture and entertainment, including the Royal Winnipeg Ballet, and the Winnipeg Symphony Orchestra, as well as the NHL Jets and CFL Blue Bombers. The comparatively low cost of living, clean air and friendly people combine to make Winnipeg a very liveable city. Winnipeg is at the interface of the prairie and boreal forest, and the Manitoba lake country offers opportunities for boating, fishing and wilderness camping. APPLICATIONS - Send curriculum vitae including a brief (one page) description of either your research experience or research interests. Promising applicants will be asked to submit a formal application. (TOEFL required for non-Canadian applicants whose native language is not English.) Send C.V's to: Dr. Brian Fristensky Department of Plant Science University of Manitoba Winnipeg, MB CANADA R3T 2N2 Phone: 204-474-6085 FAX: 204-261-5732 Email: frist@cc.umanitoba.ca >>>>> COMMUNICATION BY ELECTRONIC MAIL STRONGLY PREFERRED. <<<<<< From owner-mycology@net.bio.net Thu Apr 21 23:00:00 1994 Path: biosci!AXE.HUMBOLDT.EDU!EVERSL From: EVERSL@AXE.HUMBOLDT.EDU Newsgroups: bionet.mycology Subject: Rhododendron rust Date: 22 Apr 1994 13:31:18 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <01HBH74OBTNW8WW7G3@AXE.HUMBOLDT.EDU> NNTP-Posting-Host: net.bio.net In my study area of the Redwood National Park, I have three Rhododendrons that have fasciculations similar to those that form on Vaccinium infected with Calyptospora goeppertiana. These are in a stand of Douglas Fir/ Redwood 33 years old bordering an old growth stand of Redwood. Has anyone heard of a Rhododendron hosted rust? I appreciate your help on this. Sincerely, Linda Evers eversl@axe.humboldt.edu From owner-mycology@net.bio.net Thu Apr 21 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!agate!ihnp4.ucsd.edu!usc!sol.ctr.columbia.edu!usenet.ucs.indiana.edu!szachari@ezmail.ucs.indiana.edu From: szachari Subject: Mycologist SEEKING JOB Message-ID: Sender: daemon@usenet.ucs.indiana.edu (Usenet Server Daemon) Organization: Indiana University Date: Fri, 22 Apr 1994 14:05:04 GMT Lines: 51 I recently came from India on immigration visa and trying to establish in USA. I have considerable experience in mycological research work especially in microfungi. At present I am working as a visiting scholar in the Biology Department of Indiana University with Dr.Keith Clay. I am interested in any research positions that deals with fungi, their taxonomy, culture work, illustration , painting,etc. My brief Curriculum vitae: Simon Zachariah,Department of Biology, Indiana University, Bloomington,IN.47405. Age:42. Qualifications: Ph.D.(Soil Fungi) University of Calicut (India) Publications: 10 (Most of them in Mycologia) Membership: Life- Indian Botanical society Life- Mycological Society of India Life- Indian Society of Mycology and Plant Pathology Indian Phytopathological Society Mycological Society of America Projects accomplished: 1.Principal Investigator of the Project:Soil Flora of Nilambur Teak wood plantations (Fully Financed by the University Grants Commission India) 2.Principal Investigator of the Project:Soil Fungi of Nilambur Rubber plantations (Fully financed by the Department of Science and Technology Kerala State.) Teaching Experience: Head of the Department of Botany, Mar Thoma College Chungathara 679334, Kerala, India. (For 12 years) 1981-1993 ------------------------------------------------------------ I will be available in short notice. Contact me in my departmental address or send an email. Thank you in anticipation, Simon Zachariah From owner-mycology@net.bio.net Fri Apr 22 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!news.tamu.edu!128.194.15.2!leland From: leland@straylight.tamu.edu (Leland Ellis) Newsgroups: bionet.mycology Subject: Consensus sequences (?) in genes Date: 23 Apr 1994 00:41:45 GMT Organization: W.M. Keck Center for Genome Informatics, Institute of Biosciences and Technology, Texas A&M University, Houston Lines: 25 Distribution: world Message-ID: NNTP-Posting-Host: straylight.tamu.edu Hello, is there a compilation of the following for filamentous fungi, incl. Aspergillus nidulans: -- initiation ATGs -- splice donor and acceptor sites -- poly-A addition signals -- length of poly-A tails Thanks in advance, Leland -- Leland Ellis, Ph.D. Professor of Biochemistry and Biophysics Director, W.M. Keck Center for Genome Informatics Institute of Biosciences and Technology Texas A&M University 2121 Holcombe Houston, Texas 77030 email: leland@straylight.tamu.edu URL: http://keck.tamu.edu/ibt.html From owner-mycology@net.bio.net Fri Apr 22 23:00:00 1994 Path: biosci!fotgar.uba.edu.ar!postmaster From: postmaster@fotgar.uba.edu.ar ("Marcelo Soria") Newsgroups: bionet.mycology Subject: (none) Date: 23 Apr 1994 14:35:57 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <2db990ac.fotgar@fotgar.uba.edu.ar> NNTP-Posting-Host: net.bio.net Can anyone tell me which are the main components present in the potato infusion used for preparing potato dextrose agar or where may I find this information ? Thanks in advance. --- Marcelo Soria From owner-mycology@net.bio.net Fri Apr 22 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!pipex!uknet!ukc!rowan.ukc.ac.uk!cws From: cws@ukc.ac.uk (C.W.Scott) Newsgroups: bionet.mycology Subject: Mushrooms in the tropics Date: Sat, 23 Apr 94 18:50:43 GMT Organization: University of Kent at Canterbury, UK. Lines: 19 Sender: cws@ukc.ac.uk Distribution: world Message-ID: <2320@rowan.ukc.ac.uk> NNTP-Posting-Host: rowan.ukc.ac.uk There is much information available concerning the growing and eating of temperate zone mushrooms but where can one find similar information for the tropics. Specifically I am interested in the costal regions of North-Eastern Brasil say 9 or 10 degrees south of the equator. I know things that look like mushrooms or funghi do grow there (having seen some in a garden) but there does not appear to be a culture of eating such things. Is this because all such growths are poisonous, or is it because no-one ever got round to finding out. Any information, pointers to books/magazines/journals will be appreciated. I am a gardener rather than a scientist so information presented from that perspective would be most appreciated. Thanks in advance, Chris Scott From owner-mycology@net.bio.net Sun Apr 24 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!europa.eng.gtefsd.com!library.ucla.edu!ihnp4.ucsd.edu!news.service.uci.edu!NewsWatcher!user From: myirwin@uci.edu (Mike Irwin) Newsgroups: bionet.mycology Subject: Re: consensus seq. Followup-To: bionet.mycology Date: 25 Apr 1994 21:18:38 GMT Organization: UC Irvine, Dpt Microbio Lines: 15 Distribution: bionet Message-ID: References: <940425.08135672.078272@USM.CP6> NNTP-Posting-Host: 128.200.5.58 In article <940425.08135672.078272@USM.CP6>, GLEN_SHEARER@BULL.CC.USM.EDU (GLEN SHEARER) wrote: > > I don't know of a very recent review of Aspergillus but if you're > interested is Neurospora... > Edelmann, S.E. & C. Staben. 1994 > A statistical analysis of sequence features within genes from > Neurospora crassa. Exp. Mycol. 18:70-81. Aspergillus...Neurospora...Who cares? The Action's in Candida albicans Glen! Cheers, Mike Irwin, PSS From owner-mycology@net.bio.net Sun Apr 24 23:00:00 1994 Path: biosci!BULL.CC.USM.EDU!GLEN_SHEARER From: GLEN_SHEARER@BULL.CC.USM.EDU (GLEN SHEARER) Newsgroups: bionet.mycology Subject: RE: consensus seq. Date: 25 Apr 1994 06:13:24 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <940425.08135672.078272@USM.CP6> NNTP-Posting-Host: net.bio.net I don't know of a very recent review of Aspergillus but if you're interested is Neurospora... Edelmann, S.E. & C. Staben. 1994 A statistical analysis of sequence features within genes from Neurospora crassa. Exp. Mycol. 18:70-81. Glen From owner-mycology@net.bio.net Mon Apr 25 23:00:00 1994 Path: biosci!MERCURY.UARK.EDU!DRHOADS From: DRHOADS@MERCURY.UARK.EDU ("Doug Rhoads") Newsgroups: bionet.mycology Subject: Re: F.oxysporum_codon_usage Date: 26 Apr 1994 05:29:01 -0700 Organization: University of Arkansas Lines: 31 Sender: daemon@net.bio.net Distribution: bionet Message-ID: NNTP-Posting-Host: net.bio.net >To: mycology@net.bio.net >From: marek@pclsp2.kuicr.kyoto-u.ac.jp (Marek Tchorzewski) >Subject: F.oxysporum_codon_usage >Date sent: 26 Apr 1994 01:24:13 -0700 > >Hello Netters, > > Since I am designing primers for PCR base on amino acid >sequences, I would like to get some information about codon >usage from Fusarium oxysporum. >Does anybody out there have something like that or can point me >out in right direction. >Ones I have heard about codon usage database, is that true? > >Thanks in advance, > >Marek If you are unable to get a pre-canned codon bias table for F.o. you might consider getting a copy of SEQAID- a freeware DNA analysis program- available on many popular Mo.Bi. software servers. Then all you need are any of the known coding sequences from F.o. You can then use SEQAID to build a codon bias table. The table can be viewed, printed or used to analyze anonymous sequences for coding potential. Doug Rhoads || Dept. of Biological Sciences drhoads@mercury.uark.edu || 601 Science Engineering drhoads@uafsysb.uark.edu || University of Arkansas 501-575-3251 || Fayetteville, AR 72701 From owner-mycology@net.bio.net Mon Apr 25 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!convex!news.duke.edu!solaris.cc.vt.edu!news.ans.net!hp81.prod.aol.net!search01.news.aol.com!not-for-mail From: meeske@aol.com (Meeske) Newsgroups: bionet.mycology Subject: Re: Almost Morel time! Date: 26 Apr 1994 01:07:05 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 9 Sender: news@search01.news.aol.com Message-ID: <2pi7hp$j8v@search01.news.aol.com> References: <2p1ql7$1k6@sirius.cs.pdx.edu> NNTP-Posting-Host: search01.news.aol.com In article <2p1ql7$1k6@sirius.cs.pdx.edu>, billh@cs.pdx.edu (William J Hunt) writes: Regarding the morels: we found a few whites in elk tracks in the Willamette NF at about 2000 ft. We found no flushes of blacks, as we had the week before at that elevation, and we saw none higher either. The spring Amanitas are coming up - we found one very nice calyptroderma, and are hoping for many more. meeske@aol.com From owner-mycology@net.bio.net Mon Apr 25 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!noc.near.net!usenet.uchc.edu!neuron.uchc.edu!LPAPLAUS From: lpaplaus@neuron.uchc.edu (Leonard Paplauskas) Newsgroups: bionet.mycology Subject: Re: Almost Morel time! Date: 26 Apr 1994 11:35:25 GMT Organization: Univ of CT Health Center Lines: 26 Message-ID: <2piu9t$j5e@threed.uchc.edu> References: <2p1ql7$1k6@sirius.cs.pdx.edu>,<1994Apr20.141103.3794@ornl.gov> Reply-To: lpaplaus@neuron.uchc.edu NNTP-Posting-Host: neuron.uchc.edu In article <1994Apr20.141103.3794@ornl.gov>, matis@mars.epm.ornl.gov (Sherri Matis) writes: >Sorry guys but I can't resist gloating. > >After a few years of foraging in western Pa. and finding only enough M. esulenta >for 2 or 3 decent meals, I finally hit pay dirt. > >for the last 2 weekends I have picked over 300, big ones, small ones, even a Verpa. >On the last hunt we had stiff competion and we still found over 100. > >Mostly smaller varieties were found on the first week M. deliciosa (sic), and black morels, >some smaller esculenta. The 2nd week the esculenta were much larger, 10-15 cms. > >I think the season may be about over down here, but it should be starting very soon in Pa. >I always found giant ones about the 2nd week in may in Pa. and we are at least a month >ahead down here. > >And, I gave part of the bounty away to friends and family > >Happy hunting, >Sherri > Does anyone have an idea of morel season in south central Connecticut? From owner-mycology@net.bio.net Mon Apr 25 23:00:00 1994 Path: biosci!PCLSP2.KUICR.KYOTO-U.AC.JP!marek From: marek@PCLSP2.KUICR.KYOTO-U.AC.JP (Marek Tchorzewski) Newsgroups: bionet.mycology Subject: F.oxysporum_codon_usage Date: 26 Apr 1994 01:24:13 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <9404260826.AA17108@pclsp2> NNTP-Posting-Host: net.bio.net Hello Netters, Since I am designing primers for PCR base on amino acid sequences, I would like to get some information about codon usage from Fusarium oxysporum. Does anybody out there have something like that or can point me out in right direction. Ones I have heard about codon usage database, is that true? Thanks in advance, Marek From owner-mycology@net.bio.net Mon Apr 25 23:00:00 1994 Path: biosci!BULL.CC.USM.EDU!GLEN_SHEARER From: GLEN_SHEARER@BULL.CC.USM.EDU (GLEN SHEARER) Newsgroups: bionet.mycology Subject: RE Candida!!!!??? Date: 26 Apr 1994 08:04:29 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <940426.10052732.080180@USM.CP6> NNTP-Posting-Host: net.bio.net The action is in Candida!? Gee, I thought Histoplasma is the place to be! By the way, What's happening in Candida (exciting ) these days? From owner-mycology@net.bio.net Mon Apr 25 23:00:00 1994 Path: biosci!BORCIM.WUSTL.EDU!woods From: woods@BORCIM.WUSTL.EDU (Jon Woods) Newsgroups: bionet.mycology Subject: (none) Date: 26 Apr 1994 13:35:11 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <199404262035.AA04721@wugate.wustl.edu> NNTP-Posting-Host: net.bio.net subscribe From owner-mycology@net.bio.net Mon Apr 25 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!agate!howland.reston.ans.net!torn!nott!cunews!freenet.carleton.ca!FreeNet.Carleton.CA!as673 From: as673@FreeNet.Carleton.CA (Francois Mario Schmiedel) Subject: Re: (none) Message-ID: Sender: news@freenet.carleton.ca Reply-To: as673@FreeNet.Carleton.CA (Francois Mario Schmiedel) Organization: The National Capital FreeNet References: <2db990ac.fotgar@fotgar.uba.edu.ar> Date: Tue, 26 Apr 1994 18:09:15 GMT Lines: 20 In a previous article, postmaster@fotgar.uba.edu.ar ("Marcelo Soria") says: >Can anyone tell me which are the main components present in the >potato infusion used for preparing potato dextrose agar or where >may I find this information ? > >Thanks in advance. >--- > Marcelo Soria > > The main constituents are: potatoes, dextrose and agar. You can find a good recipe in "The Mushroom Cultivator" by Paul Stamets and J.S. Chilton. Check out Sunnisyde Book Store, they may have it. Mario Schmiedel From owner-mycology@net.bio.net Tue Apr 26 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!agate!ihnp4.ucsd.edu!swrinde!cs.utexas.edu!convex!convex!arco!news.utdallas.edu!corpgate!bnrgate!nott!cunews!freenet.carleton.ca!FreeNet.Carleton.CA!ap914 From: ap914@FreeNet.Carleton.CA (Marielle Levine) Subject: Re: Mushrooms in the tropics Message-ID: Sender: news@freenet.carleton.ca Organization: The National Capital FreeNet, Ottawa, Ontario, Canada Date: Wed, 27 Apr 1994 00:12:56 GMT Lines: 11 Try MUSHROOMPEOPLE,their e-mail no. is: mcimail.com!0002745871 @cdp.uucp also if you can find a book by Terrance McKenna who lives in California and has done work in the tropics. -- "Do I Dare Disturb the Universe?" -- T.S. Eliot "Well, it's got huge . . . very sharp . . . it can jump a . . . look at the bones." -- Tim the Enchanter From owner-mycology@net.bio.net Tue Apr 26 23:00:00 1994 Path: biosci!daresbury!trane.uninett.no!eunet.no!nuug!EU.net!howland.reston.ans.net!news.cac.psu.edu!psuvm!djr4 Organization: Penn State University Date: Wed, 27 Apr 1994 19:07:18 EDT From: Mushroom Research Lab Message-ID: <94117.190718DJR4@psuvm.psu.edu> Newsgroups: bionet.mycology Subject: Re: morels in Conn. Lines: 1 If there has been any rainfall, unlike here, they should be up right now! From owner-mycology@net.bio.net Tue Apr 26 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!pipex!uknet!daresbury!not-for-mail From: buxton@fmi.ch (Buxton Frank) Newsgroups: bionet.mycology Subject: Novozyme Date: 27 Apr 1994 13:21:04 +0100 Lines: 37 Sender: daemon@mserv1.dl.ac.uk Distribution: bionet Message-ID: <2pllbg$leo@mserv1.dl.ac.uk> Original-To: mycology@dl.ac.uk FROM: Dr. Frank Buxton CIBA GEIGY AG, K681 307 CH4002, Basel, Switzerland Tel: (Switzerland) 61 696 16 61 FAX: (Switzerland) 61 696 93 01 email buxton@fmi.ch TO: Geoff Turner email: G.Turner@sheffield.ac.uk DATE: 27 April 1994 Dear Geoff Thanks for your message. Interspex appears to be the cheapest source I have found although they charge me US$44/gm whether this reflects a change in price or this is the price they charge to nonUS and nonAustralian customers is a matter for conjecture. Some people have also recommended Glucanex available from Novo, Neumatt, CH-4243, Dittingen, Switzerland, Tel: (Switzerland) 61 765 61 11 at SwFr100 for 50g but I have not tried it yet so I cannot comment on how good it is. It may also be available from Novo in the states too. Thanks very much Dr. Frank Buxton. From owner-mycology@net.bio.net Wed Apr 27 23:00:00 1994 Path: biosci!agate!ihnp4.ucsd.edu!munnari.oz.au!ariel.ucs.unimelb.EDU.AU!ucsvc.ucs.unimelb.edu.au!lugb!sheoak.ucnv.edu.au!redgum.ucnv.edu.au!soddell Newsgroups: bionet.mycology Subject: MITES in fungal cultures Message-ID: <2pnfpc$hr3@sheoak.ucnv.edu.au> From: soddell@redgum.ucnv.edu.au (J. Soddell) Date: 28 Apr 1994 04:58:20 GMT Distribution: world Organization: La Trobe University, Bendigo NNTP-Posting-Host: redgum.ucnv.edu.au Lines: 14 We have recently encountered a mite infestation in an incubator used to grow fungi. Can anyone suddest how we get rid of the mites, and what can we do to make sure they don't come back. They're causing losts of problems with our cultures. Thanks Jacques Soddell soddell@redgum.ucnv.edu.au Biotechnology Research Centre La Trobe University, Bendigo PO Box 199 Bendigo, VIC, 3550 AUSTRALIA From owner-mycology@net.bio.net Wed Apr 27 23:00:00 1994 Path: biosci!SUMMA.TAMU.EDU!dje0282 From: dje0282@SUMMA.TAMU.EDU (Dan Ebbole) Newsgroups: bionet.mycology Subject: Fungal Genetics Meeting Date: 28 Apr 1994 06:02:14 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <199404281302.GAA21628@net.bio.net> NNTP-Posting-Host: net.bio.net Dick Weiss informs me that the Fungal Genetics Meeting in Asilomar will be March 21 to March 26 1995. ------------------------ Dr. Daniel Ebbole dje0282@summa.tamu.edu Dept. of Plant Pathology and Microbiology Texas A&M University College Station, TX 77843-2132 From owner-mycology@net.bio.net Wed Apr 27 23:00:00 1994 Path: biosci!MERCURY.UARK.EDU!DRHOADS From: DRHOADS@MERCURY.UARK.EDU ("Doug Rhoads") Newsgroups: bionet.mycology Subject: Re: close 5' ends Date: 28 Apr 1994 14:13:44 -0700 Organization: University of Arkansas Lines: 27 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <17B5893E34@uamercury.uark.edu> NNTP-Posting-Host: net.bio.net >To: mycology@net.bio.net >From: JPITKIN@msu.edu ("John.Pitkin") >Subject: close 5' ends >Date sent: 28 Apr 1994 13:04:39 -0700 > > We have recently mapped the 5' ends of two linked genes which are both >involved in HC-toxin production in Cochliobolus carbonum. These genes are >transcribed from opposite DNA strands, and are only 412 bp apart. Given the >"average" size of filamentous fungal promoters, these genes are remarkably >close together. Does anyone know of other such close genes/promoters? The >only other example I know of is the qa cluster of N. crassa, and I think the >closest genes in this cluster are c. 1 kb apart. > > > Thank you >John Pitkin >MSU-DOE Plant Research Laboratory >Michigan State University >e-mail: JPITKIN@MSU.EDU The genes for ribosomal protein S24 and L46 are divergently transcribed and are less than 630 bp apart in S. cerevisiae. Doug Rhoads || Dept. of Biological Sciences drhoads@mercury.uark.edu || 601 Science Engineering drhoads@uafsysb.uark.edu || University of Arkansas 501-575-3251 || Fayetteville, AR 72701 From owner-mycology@net.bio.net Wed Apr 27 23:00:00 1994 Path: biosci!UCDAVIS.EDU!bmtyler From: bmtyler@UCDAVIS.EDU (Brett Tyler) Newsgroups: bionet.mycology Subject: Re: close 5' ends Date: 28 Apr 1994 14:00:21 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 31 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <199404282041.NAA13494@bullwinkle.ucdavis.edu> NNTP-Posting-Host: net.bio.net At 1:04 PM 4/28/94 -0700, John.Pitkin wrote: > We have recently mapped the 5' ends of two linked genes which are both >involved in HC-toxin production in Cochliobolus carbonum. These genes are >transcribed from opposite DNA strands, and are only 412 bp apart. Given the >"average" size of filamentous fungal promoters, these genes are remarkably >close together. Does anyone know of other such close genes/promoters? The >only other example I know of is the qa cluster of N. crassa, and I think the >closest genes in this cluster are c. 1 kb apart. > > > Thank you >John Pitkin >MSU-DOE Plant Research Laboratory >Michigan State University >e-mail: JPITKIN@MSU.EDU Actually the closest genes in the qa cluster are the qa-1F and qa-1S regulatory genes which are divergently transcribed with a separation of 370 bp. In fact they share a common single binding site for the activator protein (qa-1F). In another case we are working on, all the elements for transcription of a ribsomal protein gene are located within 190 bp of the transcription startpoint. Brett Tyler Associate Professor bmtyler@ucdavis.edu From owner-mycology@net.bio.net Wed Apr 27 23:00:00 1994 Path: biosci!ADMIN.OGI.EDU!msachs From: msachs@ADMIN.OGI.EDU (Matthew Sachs) Newsgroups: bionet.mycology Subject: Re: close 5' ends Date: 28 Apr 1994 13:37:51 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 36 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <9404282038.AA20909@admin.ogi.edu> NNTP-Posting-Host: net.bio.net > We have recently mapped the 5' ends of two linked genes which are both >involved in HC-toxin production in Cochliobolus carbonum. These genes are >transcribed from opposite DNA strands, and are only 412 bp apart. Given the >"average" size of filamentous fungal promoters, these genes are remarkably >close together. Does anyone know of other such close genes/promoters? The >only other example I know of is the qa cluster of N. crassa, and I think the >closest genes in this cluster are c. 1 kb apart. > > > Thank you >John Pitkin >MSU-DOE Plant Research Laboratory >Michigan State University >e-mail: JPITKIN@MSU.EDU See: Chow, CM and RajBhandary, UL (1993) Developmental regulation of the gene for formate dehydrogenase in Neurospora crassa. J. Bacteriol. 175: 3703-3709. fdh and leu-5 are transcribed from opposite strands; they are approximately 0.6 kb apart. ----------------------------------------------------------- Matthew Sachs Department of Chemistry, Biochemistry and Molecular Biology Oregon Graduate Institute of Science and Technology 20000 NW Walker Road P.O. Box 91000 Portland, OR 97291-1000 503 690-1487 Phone 503 690-1464 Fax msachs@admin.ogi.edu From owner-mycology@net.bio.net Wed Apr 27 23:00:00 1994 Path: biosci!MSU.EDU!JPITKIN From: JPITKIN@MSU.EDU ("John.Pitkin") Newsgroups: bionet.mycology Subject: close 5' ends Date: 28 Apr 1994 13:04:39 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <199404282004.NAA11502@net.bio.net> NNTP-Posting-Host: net.bio.net We have recently mapped the 5' ends of two linked genes which are both involved in HC-toxin production in Cochliobolus carbonum. These genes are transcribed from opposite DNA strands, and are only 412 bp apart. Given the "average" size of filamentous fungal promoters, these genes are remarkably close together. Does anyone know of other such close genes/promoters? The only other example I know of is the qa cluster of N. crassa, and I think the closest genes in this cluster are c. 1 kb apart. Thank you John Pitkin MSU-DOE Plant Research Laboratory Michigan State University e-mail: JPITKIN@MSU.EDU From owner-mycology@net.bio.net Wed Apr 27 23:00:00 1994 Path: biosci!ADMIN.OGI.EDU!msachs From: msachs@ADMIN.OGI.EDU (Matthew Sachs) Newsgroups: bionet.mycology Subject: Re: MITES in fungal cultures Date: 28 Apr 1994 09:46:35 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 44 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <9404281517.AA11734@admin.ogi.edu> NNTP-Posting-Host: net.bio.net >We have recently encountered a mite infestation in an incubator used to >grow fungi. Can anyone suddest how we get rid of the mites, and what can >we do to make sure they don't come back. They're causing losts of >problems with our cultures. > >Thanks > >Jacques Soddell >soddell@redgum.ucnv.edu.au >Biotechnology Research Centre >La Trobe University, Bendigo >PO Box 199 >Bendigo, VIC, 3550 >AUSTRALIA As part of his course on Neurospora Genetics at Stanford University, David Perkins included a section on controlling mite infestations of fungal cultures He recommended procedures described in: Subden, RE and Threlkeld, SFH (1966) Mite control for Neurospora labs. Neurospora Newsl. 10: 14. Their prescription: To kill mites, freeze cultures infested with mites at temperatures below -18 degrees C for a minimum of 24 h, and treat equipment (and window perimeters) with Kelthane AP (dicofol; 1,1-bis(p-chlorophenyl)2,2,2-trichloroethanol). Best of luck. ----------------------------------------------------------- Matthew Sachs Department of Chemistry, Biochemistry and Molecular Biology Oregon Graduate Institute of Science and Technology 20000 NW Walker Road P.O. Box 91000 Portland, OR 97291-1000 503 690-1487 Phone 503 690-1464 Fax msachs@admin.ogi.edu From owner-mycology@net.bio.net Wed Apr 27 23:00:00 1994 Path: biosci!bcm!usenet From: gsmay@bcm.tmc.edu (Gregory May) Newsgroups: bionet.mycology Subject: Error in pAL4 published sequence. Date: 28 Apr 1994 16:22:08 GMT Organization: Baylor College of Medicine Lines: 19 Message-ID: <2ponrg$dja@gazette.bcm.tmc.edu> NNTP-Posting-Host: ai.cellb.bcm.tmc.edu X-Newsreader: WinVN version 0.82 Geoff Turner recently contacted me by email concerning the sequence of the expression vector pAL4. The original publication was Waring, R.B., May, G.S., and Morris, N.R. (1989) Characterization of an inducible expression system in Aspergillus nidulans using alcA and tubulin coding genes. Gene 79, 119-130. Geoff indicated that the sequence might be incorrect because they were unable to get expression from a fusion they had constructed. My laboratory has had a similar problem with a fusion from this vector. Geoff indicated that there was an additional C in their sequence not reported in the publication. We have gone back and sequenced our fusion and the original vector to determine if we had made an error. Our sequencing results confirm the prsence of an extra C. Published: ATG TCG GTA CCC GGG GAT Sequenced: ATG TCC GGT ACC CGG GGA T etc. If you or other have had a problem with this vector this may be the source of it. Greg May From owner-mycology@net.bio.net Thu Apr 28 23:00:00 1994 Path: biosci!CORNELL.EDU!bah3 From: bah3@CORNELL.EDU (Ben Horwitz) Newsgroups: bionet.mycology Subject: (none) Date: 28 Apr 1994 17:02:10 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 4 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <199404290002.UAA04148@postoffice.mail.cornell.edu> NNTP-Posting-Host: net.bio.net I would like information on how to subscribe. My lab (I am on sabbatical here) does molecular genetics of blue light transduction in filamentous fungi. From owner-mycology@net.bio.net Thu Apr 28 23:00:00 1994 Path: biosci!bcm!news.tamu.edu!128.194.15.2!leland From: leland@straylight.tamu.edu (Leland Ellis) Newsgroups: bionet.mycology Subject: Re: (none) Date: 29 Apr 1994 17:31:42 GMT Organization: W.M. Keck Center for Genome Informatics, Institute of Biosciences and Technology, Texas A&M University, Houston Lines: 22 Distribution: bionet Message-ID: References: <199404290002.UAA04148@postoffice.mail.cornell.edu> NNTP-Posting-Host: straylight.tamu.edu In-reply-to: bah3@CORNELL.EDU's message of 28 Apr 1994 17:02:10 -0700 Hello, if my memory is correct, send the one line message subscribe mycology to biosci-server@net.bio.net Cheers, Leland -- Leland Ellis, Ph.D. Professor of Biochemistry and Biophysics Director, W.M. Keck Center for Genome Informatics Institute of Biosciences and Technology Texas A&M University 2121 Holcombe Houston, Texas 77030 email: leland@straylight.tamu.edu URL: http://keck.tamu.edu/ibt.html From owner-mycology@net.bio.net Thu Apr 28 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!daresbury!trane.uninett.no!sunic!EU.net!howland.reston.ans.net!vixen.cso.uiuc.edu!usenet.ucs.indiana.edu!tclee From: tclee@silver.ucs.indiana.edu (Teng-Chun Lee) Subject: Antibiotics/antifungal qn Message-ID: Sender: news@usenet.ucs.indiana.edu (USENET News System) Nntp-Posting-Host: silver.ucs.indiana.edu Organization: Indiana University X-Newsreader: TIN [version 1.1 PL8] Date: Fri, 29 Apr 1994 17:21:11 GMT Lines: 20 [ Article crossposted from sci.med ] [ Author was Teng-Chun Lee ] [ Posted on Fri, 29 Apr 1994 05:35:11 GMT ] I am currently doing a research project on antibiotics useful in treating human fungal diseases and I came across a problem distinguishing between the terms antibiotics and antifungal agents. Can I safely assume that antifungal agents are synonymous with antibiotics used in treating fungal diseases? According to a medical dictionary definition, antibiotics are chemical substances produced by a microorganism. Therefore, I would like to know whether I can include azoles and other synthetic drugs in my study. I would appreciate it very much if someone in this field can help me shed some light on this question. Thank you very much. X-Newsreader: TIN [version 1.1 PL8] Sincerely, Teng From owner-mycology@net.bio.net Fri Apr 29 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!agate!howland.reston.ans.net!news.intercon.com!news.pipeline.com!malgudi.oar.net!mercury.wright.edu!msmith From: msmith@discover.wright.edu (Mike Smith) Subject: Morels in Ohio Message-ID: Sender: news@mercury.wright.edu (USENET News System) Organization: Wright State University, Dayton, OH 45435 X-Newsreader: TIN [version 1.2 PL2] Date: Sat, 30 Apr 1994 14:58:29 GMT Lines: 13 Has anybody had any luck finding Morels in the western/south central Ohio area? I've been checking my usual spots every 3-4 days for the last 3 weeks and have found zilch. I don't think anybody's been there first. I don't see any signs of such. So, just wondering if anybody else has had luck. Thanx, Mike Smith From owner-mycology@net.bio.net Fri Apr 29 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!agate!howland.reston.ans.net!europa.eng.gtefsd.com!emory!news-feed-2.peachnet.edu!umn.edu!news From: brambl@graz.cbs.umn.edu (Robert Brambl) Subject: Subscription information Message-ID: Sender: news@news.cis.umn.edu (Usenet News Administration) Nntp-Posting-Host: graz.cbs.umn.edu Reply-To: brambl@molbio.cbs.umn.edu (Robert Brambl) Organization: University of Minnesota, Twin Cities Date: Sat, 30 Apr 1994 18:25:43 GMT Lines: 66 Greetings, all: Several people have asked recently to have their names removed from the subscription list, usually because they prefer to read these communications through a NewsReader rather than having their mailboxes cluttered. A number of others have asked to have their names added to this e-mail subscription list. The Bionet server in the US (only) has an automated system for subscribing and unsubscribing to their newsgroups through which their server will extract your address from your subscription message and add or remove your name. The Daresbury server is manually tended. The following procedures should work, depending upon where you live: If you are located in Europe, Africa, or Central Asia, please contact biosci@daresbury.ac.uk and request a subscription to MYCOLOGY. If you are located in the Americas or the Pacific Rim, log in to the computer account in which you would like to receive mail (not an account that you use infrequently) and send a mail message to the