From owner-mycology@net.bio.net Thu Sep 01 23:00:00 1994 Path: biosci!rutgers!gatech!newsfeed.pitt.edu!toads.pgh.pa.us!hudson.lm.com!tusk.lm.com!not-for-mail From: Richard W. Kerrigan Newsgroups: bionet.mycology Subject: Re: Mycorrhiza in the tropics Date: 2 Sep 1994 08:55:04 -0400 Organization: Telerama Public Access Internet, Pittsburgh, PA Lines: 15 Sender: sylvan@telerama.lm.com Message-ID: <3477b8$h8l@tusk.lm.com> References: <3424iq$jb0@kaos.deepcove.com> NNTP-Posting-Host: tusk.lm.com X-Newsreader: TIN [version 1.2 PL2] Alex King (alexking@deepcove.com) wrote: : Hello, I am looking for any information on the role of mychorrhiza in : nutrient cycling in tropical ecosystems. Any help would be appreciated. : Thank You, : Alex King : alex.king@deepcove.com In addition to the volumes of information on this subject about which I know nothing... A few years ago, Nalini Nadkarni was taking a look at the role of mycorrhizae of aerial roots in the tropical canopy. Seemed interesting to me. I believe that she is at Evergreen College in WA now. Good luck. -- Rick Kerrigan (rwk@sylvanres.com) From owner-mycology@net.bio.net Thu Sep 01 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!convex!news.duke.edu!news-feed-1.peachnet.edu!gatech!howland.reston.ans.net!news.cac.psu.edu!news.tc.cornell.edu!travelers.mail.cornell.edu!newstand.syr.edu!gamera.syr.edu!griffin From: griffin@gamera.syr.edu (David H. Griffin) Newsgroups: bionet.mycology Subject: DNA estimation Date: 2 Sep 1994 13:21:02 GMT Organization: Syracuse University, Syracuse, NY Lines: 16 Distribution: world Message-ID: <3478ru$8o0@newstand.syr.edu> NNTP-Posting-Host: gamera.syr.edu Sorry, this is a repeat submission with my name attached this time. Another method for estimating nuclear DNA content, providing you have some reasonable basis for determining ploidy, is reassociation kinetics of the DNA. This is probably the most accurate method, but like all methods is subject to understanding the artifacts and interferences that are involved. I think that estimation from chromosomal electrophoresis (CHEF, OFAGE) would be the least accurate since not only must ploidy be assumed, but the assumptions made for estimating chromosomal standards used for comparisons are not very precise. David H. Griffin State University, College of Environmental Science & Forestry Syracuse NY griffin@mailbox.syr.edu From owner-mycology@net.bio.net Thu Sep 01 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!convex!news.duke.edu!news-feed-1.peachnet.edu!gatech!howland.reston.ans.net!news.cac.psu.edu!news.tc.cornell.edu!travelers.mail.cornell.edu!newstand.syr.edu!gamera.syr.edu!griffin From: griffin@gamera.syr.edu (David H. Griffin) Newsgroups: bionet.mycology Subject: uencoding Date: 2 Sep 1994 13:20:16 GMT Organization: Syracuse University, Syracuse, NY Lines: 12 Distribution: world Message-ID: <3478qg$8n6@newstand.syr.edu> NNTP-Posting-Host: gamera.syr.edu Sorry, this a repeat submission with my name attached this time. I can't translate uencoding either. Surely if contributors feel that some particular information they have can only be sent by this method, they could indicate the nature of the information and offer to send it via e-mail to anyone expressing particular interest. Otherwise, lets keep this limited to messages readily available to all. David H. Griffin State University, College of Environmental Science & Forestry Syracuse NY griffin@mailbox.syr.edu From owner-mycology@net.bio.net Thu Sep 01 23:00:00 1994 Path: biosci!biol.rug.nl!SCHURENF From: SCHURENF@biol.rug.nl (SCHURENF) Newsgroups: bionet.mycology Subject: transcription termination/poly-adenylation Date: 2 Sep 1994 07:08:23 -0700 Organization: Department of Biology, RUGroningen Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hello researchers, If you feel there is too much mushroomhunting in this group just add some research messages. I was wondering if anyone ever looked in some detail into terminators of transcription in filamentous fungi? As far as I know no consensus sequences have ever been found or described, but there may be some general features about these sequences that people have noticed. If you have any information please contact me. Frank Schuren Dept. Plant Biology University of Groningen schurenf@biol.rug.nl From owner-mycology@net.bio.net Thu Sep 01 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!swrinde!howland.reston.ans.net!news.cac.psu.edu!news.tc.cornell.edu!travelers.mail.cornell.edu!newstand.syr.edu!gamera.syr.edu!griffin From: griffin@gamera.syr.edu (David H. Griffin) Newsgroups: bionet.mycology Subject: Mycology teaching Date: 2 Sep 1994 13:22:05 GMT Organization: Syracuse University, Syracuse, NY Lines: 17 Distribution: world Message-ID: <3478tt$8o6@newstand.syr.edu> NNTP-Posting-Host: gamera.syr.edu My approach to teaching mycology lab is to have students work together in groups on several projects. These include making a collection of fungi identified to genus, studying the development of Coprinus cinereus and Ophiostoma ulmi in pure culture in which the students do everything from media preparation, maintenance of stock cultures, single spore isolations, matings to determine mating type and setting up cultures to obtain both asexual and sexual life cycle stages. There is an exercise on isolation of phylloplane fungi that includes selective conditions for shadow yeasts, surface molds, and endophytic fungi. We also make beer and tempeh. A few demonstrations of fungal morphology are made from time to time to fill in some gaps. David H. Griffin State University, College of Environmental Science & Forestry Syracuse NY griffin@mailbox.syr.edu From owner-mycology@net.bio.net Thu Sep 01 23:00:00 1994 Path: biosci!rutgers!gatech!howland.reston.ans.net!cs.utexas.edu!not-for-mail From: maxwell@calshp.cals.wisc.edu Newsgroups: bionet.mycology Subject: Selective media-Mycosphaerella Date: 2 Sep 1994 11:02:52 -0500 Organization: UTexas Mail-to-News Gateway Lines: 12 Sender: nobody@cs.utexas.edu Message-ID: <9409021603.AA04265@calshp.cals.wisc.edu> NNTP-Posting-Host: news.cs.utexas.edu Does anyone out there know of any selective, or semi-selective media used to isolate fungi in the genus Mycosphaerella (or anything close)? I am working on Mycosphaerella populorum (anamorph=Septoria musiva) which causes disease on Populus trees. I have searched the literature, but was hoping I might find someone with unpublished knowledge about this subject. Please respond to my email address since my access to this group is limited. thanks. -- David Maxwell 382 Russell Labs maxwell@calshp.cals.wisc.edu 1630 Linden Drive dlm@plantpath.wisc.edu From owner-mycology@net.bio.net Thu Sep 01 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!gatech!news-feed-1.peachnet.edu!news.duke.edu!solaris.cc.vt.edu!uunet!newstf01.cr1.aol.com!search01.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: Re: Need help!- first time mushroom grower Date: 2 Sep 1994 18:23:04 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 9 Sender: news@search01.news.aol.com Message-ID: <3488k8$336@search01.news.aol.com> References: <30sps6$a59@search01.news.aol.com> NNTP-Posting-Host: search01.news.aol.com In article <30sps6$a59@search01.news.aol.com>, reefabeefa@aol.com (Reefabeefa) writes: jeff- first, this is not an advertisement (I have been admonished more than once) BUT you should get my latest book, Growing Gourmet & Medicinal Mushrooms which most major bookstores are carrying. good luck paul stamets From owner-mycology@net.bio.net Thu Sep 01 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!gatech!news-feed-1.peachnet.edu!news.duke.edu!solaris.cc.vt.edu!uunet!newstf01.cr1.aol.com!search01.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: what do mycologists get paid these days? Date: 2 Sep 1994 18:21:02 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 18 Sender: news@search01.news.aol.com Message-ID: <3488ge$326@search01.news.aol.com> NNTP-Posting-Host: search01.news.aol.com I am interested in knowing the range of salaries for mycologists skilled in sterile technique and contaminant identification. Can anyone provide the general salary range common to the industry? furthermore, i need to find a data base listing species names of basidiomycetous fungi tied to the author citations and the original publications. is there such a data base? if so, how does a neophyte on Internet find it? mucho gracias amigos paul stamets From owner-mycology@net.bio.net Thu Sep 01 23:00:00 1994 Path: biosci!DARTMOUTH.EDU!Mari.L.Shinohara From: Mari.L.Shinohara@DARTMOUTH.EDU (Mari L. Shinohara) Newsgroups: bionet.mycology Subject: subscription Date: 2 Sep 1994 07:08:37 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <12257333@prancer.Dartmouth.EDU> NNTP-Posting-Host: net.bio.net I'd like to subscribe this. Thank you. Mari From owner-mycology@net.bio.net Fri Sep 02 23:00:00 1994 Path: biosci!daresbury!trane.uninett.no!eunet.no!nuug!EU.net!howland.reston.ans.net!europa.eng.gtefsd.com!swiss.ans.net!newstf01.cr1.aol.com!search01.news.aol.com!not-for-mail From: mycoworld@aol.com (MycoWorld) Newsgroups: bionet.mycology Subject: Re: ganoderma lucidum Date: 2 Sep 1994 23:43:11 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 14 Sender: news@search01.news.aol.com Message-ID: <348rcf$83n@search01.news.aol.com> References: NNTP-Posting-Host: search01.news.aol.com In article , mgalatz@panix.com (Menachem Galatz DC) writes: Yes, as Bob Harris noted, Ganoderma lucidum can be cultivated. In fact there is at least one commercial operation in the US producing it for use in some kind of "health" pill. It's typically used to make a tea. Whether the tea has any health benefits or not is a matter for research, but it does taste good. It's commonly known as reishi. It has an interesting history in the orient. See "Reishi Mushroom - Herb of Spiritual Potency and Medicinal Wonder" by Terry Willard (ISBN = 0-9625638-0-3) at $14.95 in paper. For info on cultivation see Paul Stamets' books (Paul follows this newsgroup) . -Jerry- Mycoworld@aol.com From owner-mycology@net.bio.net Sat Sep 03 23:00:00 1994 Path: biosci!daresbury!trane.uninett.no!eunet.no!nuug!EU.net!uunet!newstf01.cr1.aol.com!search01.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: Re: Amateur Mushroom growing Date: 3 Sep 1994 19:03:06 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 13 Sender: news@search01.news.aol.com Message-ID: <34avba$n10@search01.news.aol.com> References: <33h7sl$a8g@odin.community.net> NNTP-Posting-Host: search01.news.aol.com two journals dedicated to amateurs with emphasis on mushroom cultivation: Mushroom, the Journal Box 3156 Moscow, Id. 83843 Mushrooms Grower's Newsletter 5140 Lombardy Lane Klamath Falls, Or. 97603 good luck. if you want more info. send me a mailing address. paul stamets From owner-mycology@net.bio.net Sat Sep 03 23:00:00 1994 Path: biosci!daresbury!trane.uninett.no!eunet.no!nuug!EU.net!uunet!newstf01.cr1.aol.com!search01.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: Re: searching anything about mushrooms Date: 3 Sep 1994 18:48:05 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 8 Sender: news@search01.news.aol.com Message-ID: <34auf5$ms6@search01.news.aol.com> References: NNTP-Posting-Host: search01.news.aol.com In article , sabba@myhost.subdomain.domain (Riccardo Sabbatini) writes: We have alot of information on mushrooms. Send us your mailing address and we will send you a free catalogue. paul stamets "stamets1aol.com." From owner-mycology@net.bio.net Sat Sep 03 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!daresbury!trane.uninett.no!nac.no!eunet.no!nuug!EU.net!uunet!newsflash.concordia.ca!sifon!bio1.lan.mcgill.ca!gilbert From: gilbert@bio1.lan.mcgill.ca (Gilbet Cabana) Subject: Stable isotopes in ecological mycology Message-ID: Lines: 17 Sender: news@sifon.cc.mcgill.ca Organization: McGill University X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Date: Sat, 3 Sep 1994 22:07:55 GMT I am currently conducting research on aquatic food chains using carbon and nitrogen stable isotope ratios and as a side project has been using these techniques to study forest fungi. Briefly, carbon isotope ratios measures the relative abundance of C13 to C12. This ratio ("signature") is carried from primary producers to consumers, to secondary consumers, etc. along food chains. Since mushrooms do not fix carbon, the should have the carbon isotope signature of their host. This technique could be used to identify mushrooms which are obligate "parasites" of a specific species of tree (trees species can differ in their C-signatures) from those mushrooms species that are generalists, obtaining their carbon from a variety of organic sources. My question is: Do people know about studies on host-specificity in mushrooms (basidios, ascos..)? Thanks for your time, Gilbert From owner-mycology@net.bio.net Sat Sep 03 23:00:00 1994 Path: biosci!daresbury!trane.uninett.no!eunet.no!nuug!EU.net!uunet!newstf01.cr1.aol.com!search01.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: Re: Commercializing the net Date: 3 Sep 1994 19:06:09 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 12 Sender: news@search01.news.aol.com Message-ID: <34avh1$n2d@search01.news.aol.com> References: <199408251454.HAA04501@net.bio.net> NNTP-Posting-Host: search01.news.aol.com In article <199408251454.HAA04501@net.bio.net>, DJJACOB@MSU.EDU ("David.Jacobson") writes: thanks for your comments. I am new to this so I will try to walk a faithful line delimiiting commercialization and the intended purpose of the NET. Please accept my apologies if I stepped on anyone's toes. paul stamets ps: how do I remove my "Paul Stamets & Fungi Perfecti" notice??? Scrolling down all the menu options has failed to reveal the subroutine. From owner-mycology@net.bio.net Sat Sep 03 23:00:00 1994 Path: biosci!daresbury!trane.uninett.no!nac.no!eunet.no!nuug!EU.net!uunet!newstf01.cr1.aol.com!search01.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: Re: Cultivation of Cantharellus cibarius, mykhorrhiza Date: 3 Sep 1994 19:09:05 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 7 Sender: news@search01.news.aol.com Message-ID: <34avmh$n49@search01.news.aol.com> References: NNTP-Posting-Host: search01.news.aol.com I remember a reference from a Sweedish or Norwegian mycologist who was able to get Cantharellus cibarius spores to germinate in the presence of a red yeast named Rhodotorula glutinus (sp?). Cantharellus mycelium has been slow for me. anyone else remember this reference? paul stamets From owner-mycology@net.bio.net Sun Sep 04 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!pipex!sunic!trane.uninett.no!eunet.no!nuug!EU.net!uunet!senior.nectec.or.th!mars.mahidol.ac.th!mucc!g3636197 From: g3636197@mucc.mahidol.ac.th (Preecha Leangaramgul - SCBT - 3636197) Newsgroups: bionet.mycology Subject: cDNA synthesis Date: 5 Sep 1994 07:43:39 GMT Organization: Mahidol University, Thailand. Lines: 12 Message-ID: <34ei7b$8fg@mars.mahidol.ac.th> NNTP-Posting-Host: 202.14.162.1 Keywords: cDNA, mRNA, A.niger X-Newsreader: TIN [version 1.2 PL2] To members of newsgroup I am going to construct a cDNA library of a fungus which I have never done before. I have some doubts about the use of PCR (Polymerase Chain Reaction) based technique versus the conventional method (poly A+ mRNA isolation --> reverse transcription --> cloning). I would also like to know which commercially available cDNA synthesis kit is the most effective one. Thank you very much Preecha Leangaramgul 3636197@mucc.mahidol.ac.th From owner-mycology@net.bio.net Sun Sep 04 23:00:00 1994 Path: biosci!MUSICA.MACARTHUR.UWS.EDU.AU!ABMB From: ABMB@MUSICA.MACARTHUR.UWS.EDU.AU (Miroslav Belik) Newsgroups: bionet.mycology Subject: (none) Date: 5 Sep 1994 05:36:00 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <05SEP94.24370029.0012.MUSIC@MUSICA.MACARTHUR.UWS.EDU.AU> NNTP-Posting-Host: net.bio.net unsubscribe miroslav belik MIROSLAV BELIK E-MAIL M.BELIK@UWS.EDU.AU UNIVERSITY WESTERN SYDNEY, MACARTHUR. TEL. 61-46-203-520 FAC. BUS. & TECH. FAX. 61-46-266-683 P.O. BOX 555, CAMPBELLTOWN NSW 2560 AUSTRALIA From owner-mycology@net.bio.net Mon Sep 05 23:00:00 1994 Path: biosci!daresbury!not-for-mail From: buxton@fmi.ch (Buxton Frank) Newsgroups: bionet.mycology Subject: email Date: 6 Sep 1994 15:42:14 +0100 Lines: 7 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <34hv46$1g6@mserv1.dl.ac.uk> Original-To: mycology@dl.ac.uk The email to post on this newsgroup is also: mycology@dl.ac.uk Frank Buxton email: buxton@fmi.ch From owner-mycology@net.bio.net Mon Sep 05 23:00:00 1994 Path: biosci!BIO.TAMU.EDU!TOM From: TOM@BIO.TAMU.EDU ("Tom Adams") Newsgroups: bionet.mycology Subject: email Date: 6 Sep 1994 06:14:48 -0700 Organization: Texas A&M University - Biology Dept Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: Reply-To: Tom@bio.tamu.edu NNTP-Posting-Host: net.bio.net The email address to post on this newsgroup is: mycology@net.bio.net Tom Adams Department of Biology Texas A&M University College Station, TX 77843 409-845-1468 Tom@bio.tamu.edu From owner-mycology@net.bio.net Mon Sep 05 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!pipex!warwick!news.shef.ac.uk!sunc!mb1gt From: mb1gt@sunc.sheffield.ac.uk (G Turner) Newsgroups: bionet.mycology Subject: Abstract:how to submit? Date: 6 Sep 1994 07:35:00 GMT Organization: Academic Computing Services, Sheffield University Lines: 7 Message-ID: <34h634$jh3@hippo.shef.ac.uk> NNTP-Posting-Host: sunc.shef.ac.uk X-Newsreader: TIN [version 1.2 PL2] Is it possible to send a file to bionet.mycology via the reader (similar to posting messages like this one) without subscribing. If so, which command? If not, what is the email address of bionet? Typing out an abstract using this system is too laborious. Geoff Turner G.Turner@sheffield.ac.uk From owner-mycology@net.bio.net Mon Sep 05 23:00:00 1994 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!library.ucla.edu!europa.eng.gtefsd.com!swiss.ans.net!newstf01.cr1.aol.com!search01.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: Re: Fungus tea Date: 6 Sep 1994 18:42:05 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 16 Sender: news@search01.news.aol.com Message-ID: <34ir7t$nk0@search01.news.aol.com> References: <34ihft$ml0@mserv1.dl.ac.uk> NNTP-Posting-Host: search01.news.aol.com In article <34ihft$ml0@mserv1.dl.ac.uk>, Don Reynolds writes: the fungus tea you mention might be referring to one called the "Manchurian Mushroom" or Chambucha, Kambuska, etc.... I have grown it and have lots of information on it. It is very popular with the New Agers who would rather drink a substance of unknown identity than to learn that it may kill you. seriously. give me your address and i will send you an info sheet on it. paul stamets From owner-mycology@net.bio.net Mon Sep 05 23:00:00 1994 Path: biosci!daresbury!not-for-mail From: Don Reynolds Newsgroups: bionet.mycology Subject: Fungus tea Date: 6 Sep 1994 20:55:41 +0100 Lines: 19 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <34ihft$ml0@mserv1.dl.ac.uk> MIME-Version: 1.0 Original-To: mycology@dl.ac.uk I seek current information on Karagasok Tea, Teekwass etc. I am aware of the references in the 7th edition of the Dictionary of the Fungi. I have the article by F. R. Petersen, 1986, from Foren. svam. fremm. 14:66-76. Here in the Los Angeles area a preparation is being sold commercially. A local magazine is interested in this product and its contemporary uses. Is the any validation of medicinal benefit from this preparation? Are other commercial sources available? Does anyone use this preparation on a regular basis? Thanks -- dreynold@mizar.usc.edu Don R. Reynolds Telephone 213 744 3232 Research and Collections FAX 213 744 3482 or 213 746 2999 Natural History Museum 900 Exposition Boulevard Los Angeles, California 90007 From owner-mycology@net.bio.net Tue Sep 06 23:00:00 1994 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!sdd.hp.com!nobody From: maschue@sdd.hp.com (Sean Maschue) Newsgroups: bionet.mycology Subject: Kombucha Tea fungus ala Otto Lang Followup-To: bionet.mycology Date: 6 Sep 1994 17:21:19 -0700 Organization: Center for Misogynistic Advancement, San Diego Division Lines: 283 Distribution: world Message-ID: <34j11vINNpc2@hpsdleb3.sdd.hp.com> NNTP-Posting-Host: hpsdleb3.sdd.hp.com Someone here asked about the tea fungus. Otto Lang, had put together the following information on the elixir. I found it very intersting. I hope you enjoy it. Thanks to Otto! -------------------------------------------------------------- KOMBUCHA ELIXIR or MANCHURIAN TEA edited by Otto Lang There is apparently an entrepreneur in Germany who provides not only a recipe, but also the ingredients, for this tonic tea, which is said to improve good health and longevity. His address appears at the end of this article. A bit of background: In September 1993, at the annual Fungi Fair of the Mycological Society of Toronto, a couple of Russian-Canadian descendants asked me whether I knew the scientific name of the fungus used in 'kombucha', a fermented tea with antibiotic effects. Fungus? - I never even heard of the tea, never mind the fungus. I began to investigate. Not only did I post a query on INTERNET to the North American mycologcial community, but I also asked around at the Annual Foray of the North American Mycological Association at Ford Worden, Washington in October of that year. Finally, nine months after the initial inquiry, I received a fax from Larry Stickney, San Francisco Mycological Society, of what looks like an advertorial by an unknown author, citing the material of the above mentioned German outfit. It describes the alleged benefits, a recipe and some 'scientific' descriptions and characteristics, which I pass on without any specific endorsement or warranty. Some of the remedial claims are repetitive and appear to be "reaching". The scientific claims are not supported by verifiable references or bibliographic sources. And the name? Stickney reports a Larry Evores calling this "apparently a species of ~Oxyporus~". I doubt that, since my 1983 Dictionary of Fungi, defines ~Oxyporus~ (H. Bourdot & Galzin) Donk (1933) as the 7th genus in Polyporaceae; Persoonia 4:342,1966. This does not match the description of the organism below. But ~Pichia fermentans~, mentioned in the article, sounds like a reasonable guess. ~Pichia~, Hansen (1904), is one of 30 genera in the Saccharomycetaceae, the 4th family in the Endomycetales, a group of teleomorphic yeasts in the Ascomycotina. See Von Arx et al (Stud.mycol.14:1977) and others. The following is a verbatim copy of the fax received, and since it is an advertisement with the disclosure of sources I don't think I am infringing on any copyright. I also acknowledge that the writing is neither European, nor Canadian, but American in style, and, if you are not a believer at least, it should be good for your facial muscles and your solar plexus. ROTFL ---------- O.L. =*=*= Kombucha Elixir =============== Youth! Beauty! Health! The timeless quest! But where is it to be found? It is well documented that there are pockets of people who are centenarians, clustered not only in the mountainous Caucasias, where they are most celebrated, but also in rural areas like Yakutia in Siberia, or the Poltys District of the Ukraine, as well as Tibet and Spain. Compare this to the elderly in the United States, who are often medicated and placed in nursing homes resigned to dying, without even thinking of reaching the age of 100. A centenarian in the US is interviewed, congratulated by the President and given a party in his honor at his nursing home, so great is the occasion. These centenarians in Russia, Spain and Tibet are actively involved in life even at their late age. The harmony prevalent in Kargasok society is not reproduced in our Western world of stress and fast-paced living. The Russian centenarians attribute their longevity not only to their work habits, but also to the _Yeast Enzyme Tea_ which has been in their diet for hundreds of years. This tea contains a concentrated amount of _high quality protein_ which the body ingests and uses immediately. Yeast enzymes respond to the living micro-organisms in the body where they metabolize readily. _This ancient yeast-culture tea_, which the Karagsoks believe sustains their excellent health into their hundreds, found its way years ago back to Japan again, when a prominent Japanese lady traveled to Kargasok, Russia. Upon arrival there she made an extraordinary observation of an unusually healthy people. The women were virtually without wrinkles and few other visible signs of aging. It was disclosed to her that everyone drank about eight ounces of kumbucha tea daily. To make this tea, the Japanese lady obtained a piece of the special yeast culture with instructions on how to make it. After her friends and her driniking it for several weeks, these friends reported an astonishing difference in how they felt physically. Her reports also claim the pronounced lowering of blood pressure, the fading of wrinkles, the gradual beginning of hair restoration and an overall feeling of well-being. TODAY, OVER A MILLION JAPANESE PEOPLE DRINK THE FERMENTED TEA DAILY. It is a common conversation piece on television and over the radio. =*=*= Kombucha works like a natural antibiotic ======================================== The Kombucha IX, or Kargasok 110, has been used for many hundreds, maybe thousands of years by those who believe it can activate the glandular system, stimulate the metabolism, reduce weight, lower uric acid, cholesterol, rheumatism, arthritis, neurasthenic stomach- kidney-liver disorders, etc. The tea is also felt to rebuild the colon flora, increase the blood circulation and aid those under stress. We are talking about a "Panacea" Manchurian Mushroom that takes seven days to reproduce itself; it looks like a 6" diameter hot cake but is grayish in color. Properly cultivated, it: 1.Eliminates wrinkles and helps removal of brown spots on hands. It is skin humectant. 2.Prevents certain types of cancer. In Manchuria, where this mushroom came from, there has not been detected one single case of cancer. Every day people drink this tea as a religious atonement. 3.During menopause, it reduces hot-flash discomforts. Just after drinking Manchurian Tea you may feel a warm sensation due to the fact that the tea components join the blood stream causing a draining action of toxic chemical elements and fluids, reason for which (sic) you will notice increased mobility in your extremities and flexibility around your waist. 4.Helps constipation 5.Helps muscular aches and pains in shoulders and neck 6.Helps bronchitis, asthma, and coughs, in two or three days. Will halp children with phlegm. 7.Helps with allergies, also with aching nerves. 8.It is prescribed in kidney problems 9.It has proven useful in cataracts and other formations on cornea. 10.It cleanses the gall bladder, helps colitis and nervous stomachs. 11.Helps heal diseases. It will lower cholesterol and _soften veins and arteries_. 12.It will stop infectous diarrhea. 13.Helps burning of fat, therefore, helps lose weight. 14.Helps insomnia. 15.Helps the liver work more efficiently. 16.Helps to level off glucose and sudden drops of blood sugar in diabetics. If taken daily it will eliminate urea in 100 days. 17.It has surprising effects on the scalp, helps avoid balding, thickens hair and eliminates gray hair. 18.Helps digestion. Dr. Pan Pen, a scientist of Japan, reports his findings as follows: The tea helps restore hair growth after 4 -6 months and gray hair darkens; and eyesight is strengthened. Because it was found that _the culture contains three basic vital elements_ needed by the body, it is claimed that the tea is a life extender. Dr Sklenar of Germany is using this tea to support cancer treatments. He is also using Kombucha IX and some other remedies to aid in cancer treatments and he believes that this tea can prevent cancer if drunk daily. The first recorded use of Kombucha Tea was during the Chinese empire of the Tsin-Dynasty in 221 B.C. It was referred to as "The Remedy for Immortality" or "The Divine Tshe". In 414 B.C. Dr. Kombu, from Korea, brought it to Japan during the reign of Emperor Inkyo. ('Cha' is the Cantonese word for tea. Hence 'Kombu-cha'. Ed.) Afterwards, this tea was used throughout China, Japan and Korea and later introduced into Russia and India. It was called by several different names: "Fungus japonicus", "Fungo-japon Kombucha", ~Pichia fermentans~, "Cembuya orientalis", "Combuchu", "Tschambucco", "Volga Spring", "mo-Gu", "Champignon de longue vie", "Teekwass", "Kwassan", and "Kargasok Tea". The kombucha fungus is built in membrane form and is a symbiosis of yeast cells and different bacteria. Among these bacteria are: - Bacterium xylinum, Bacterium gluconicum, Acetobacter ketogenum and pichia fermentans. (The latter is _not_ a bacterium!. Ed.) The kombucha fungus needs to live in a solution composed of black tea and sugar. In the right temperature they multiply constantly. They don't build spores as yeast normally does, but instead multiply by a process producing glucuronic acid, lactic acid, acetic acid and several vitamins. The yeast culture transforms the sugar and black tea into enzymesuseful for the body. The fermented mixture later contains not only these products, but also 0.5% alcohol. _Glucuronic acid_ is used in the body to build the improtant polyscharides such as: Hyaluronic acid which is vital for the connective tissues; Chondroitinsulfat acid which is the basic substance in our cartilage; Mykoitinsulfat acid which is for the mucous and for the virtreous (eye) and also Heparin and Lactic acid which is especially for our colon. =*=*= How to prepare kombucha tea =========================== Ingredients 1) One kombucha mushroom of about 3 inches in diameter. 2) Two litres (3 1/2 quarts) of fresh water (preferably natural mineral). 3) 15 grams (two tablespoons) biological green tea (Chinese or Japanese) 4) 200 grams (7 ozs.) refined white sugar (Some alternate recipe I've seen recommends light brown sugar.Ed.) 5) A stainless steel saucepan (do _not_ use aluminum) 6) A clean glass container with a wide mouth which will hold a 3" diameter mushroom and 2 litres of water 7) A piece of cheesecloth and a rubber band to close the jar A - The initial process - How to start the culture 1) Boil the water, remove the saucepan from the heat 2) Let it cool down for about 5 minutes 3) Add the tea and let it steep for about 15 minutes, then remove the tea bag. 4) Add the sugar and stir thoroughly 5) Let the tea cool down to about 90 degrees Fahrenheit 6) When making the first culture: Add two tablespoons of natural vinegar (which you omit when you already have mombucha batch made) 7) Pour the tea into the glass container and add the kombucha mushroom 8) Close the top with the cloth and rubber band. 9) Place the jar in a spot at a temperature of 80 degrees F. 10) After 10 to 14 days a new kombucha mushroom will have grown on the whole surface of the tea. 11) This kombucha tea should be strained, bottled, sealed with a clean lid and kept in the refrigerator. B - the subsequent process You make the new batch of tea with the new mushroom (which has grown on top of the old mushroom in the previous batch). The old mushroom can be used to fertilize plants, applied to wounds or used as cosmetic. When the new tea has cooled and been poured into the jar it should be mixed with about 8 oz. per quart of the previous (This sounds unreasonable, probably 'per litre'. Ed.) batch of kombucha tea. This favors the fermentationas a result of which the new kombucha tea will be ready in 8 to 10 days. Of course you have to put the kombucha mushroom in the cool tea and proceed as in step A above. Recommended dosage: 4 oz. every morning, on an empty stomach. Note: This recipe is the result of - centuries of international experience - scientific research - years of verification R. Frank Kombucha Zucht und Forschung Genossenschafts Str. 10 D-75217 Birkenfeld/Wuertt. Allemagne, Germany Tel:7231-47810 Fax 7231-485046 =*=*=* This copy was made for electronic distribution 94-06-03. Otto Lang, IBM ret., Amateur mycologist Member of the Mycological Society of Toronto (MST) Member of the North American Mycological Association (NAMA) Member of the Mycological Society of America (MSA) Member of the Personal Computer Club of Toronto (PCCT) From owner-mycology@net.bio.net Tue Sep 06 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!quagga.ru.ac.za!nntp.und.ac.za!pc057.ag.unp.ac.za!TennentG From: TennentG@micr.unp.ac.za (Geoff.Tennant) Newsgroups: bionet.mycology Subject: Re: Fungus tea Date: Wed, 7 Sep 1994 06:24:02 GMT Organization: University of Natal , Pietermaritzburg, South Africa Lines: 33 Message-ID: References: <34ihft$ml0@mserv1.dl.ac.uk> <34ir7t$nk0@search01.news.aol.com> NNTP-Posting-Host: pc057.ag.unp.ac.za In article <34ir7t$nk0@search01.news.aol.com> stamets1@aol.com (Stamets 1) writes: >From: stamets1@aol.com (Stamets 1) >Subject: Re: Fungus tea >Date: 6 Sep 1994 18:42:05 -0400 >In article <34ihft$ml0@mserv1.dl.ac.uk>, Don Reynolds > writes: >the fungus tea you mention might be referring to one called the >"Manchurian >Mushroom" or Chambucha, Kambuska, etc.... >I have grown it and have lots of information on it. >It is very popular with the New Agers who >would rather drink a substance of unknown >identity than to learn that it may kill you. >seriously. give me your address and i >will send you an info sheet on it. >paul stamets Paul, This fungus tea was all the rage here in about 1990, but the craze died out very quickly. I can't help but feel that the article by R. Frank (posted by Sean Maschue) sounds like one of those TV mail order advertisements (i.e. too good to be true). I have allways thought that there must be some serious mycotoxins in this gunk. Are there? Geoff Tennant University of Natal South Africa From owner-mycology@net.bio.net Tue Sep 06 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!ee.und.ac.za!nntp.und.ac.za!pc057.ag.unp.ac.za!TennentG From: TennentG@micr.unp.ac.za (Geoff.Tennant) Newsgroups: bionet.mycology Subject: Re: Need help!- first time mushroom grower Date: Wed, 7 Sep 1994 06:07:46 GMT Organization: University of Natal , Pietermaritzburg, South Africa Lines: 31 Message-ID: References: <30sps6$a59@search01.news.aol.com> <3488k8$336@search01.news.aol.com> NNTP-Posting-Host: pc057.ag.unp.ac.za In article <3488k8$336@search01.news.aol.com> stamets1@aol.com (Stamets 1) writes: >From: stamets1@aol.com (Stamets 1) >Subject: Re: Need help!- first time mushroom grower >Date: 2 Sep 1994 18:23:04 -0400 >In article <30sps6$a59@search01.news.aol.com>, reefabeefa@aol.com >(Reefabeefa) writes: >jeff- first, this is not an advertisement (I have been admonished more >than once) >BUT you should get my latest book, Growing Gourmet & Medicinal >Mushrooms which most major bookstores are carrying. good luck >paul stamets Dear Paul, I am currently experimenting with various media as casing for the production of Agaricus bisporus and was wondering what have you found to be the most successful? I have so far got some fantastic results: outyielding the local South African peat (which is more like mud than anything) by as much as 35% in the first flush. I have not as yet obtained the data for subsequent flushes, but the highly productive casing media still look great. What is the most commonly used medium in your area/country? Also, in selecting media, what do you consider to be its most important attributes? (I have concentrated on porosity and water-holding capacity). Geoff Tennant University of Natal South Africa From owner-mycology@net.bio.net Tue Sep 06 23:00:00 1994 Path: biosci!CHEM.UCLA.EDU!rlw From: rlw@CHEM.UCLA.EDU (Richard Weiss) Newsgroups: bionet.mycology Subject: Re: Fungus tea Date: 7 Sep 1994 07:38:04 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: <9409071433.AA00982@uclachem.chem.ucla.edu> NNTP-Posting-Host: net.bio.net It would benefit all subscribers to mycology@net.bio.net if responses to individual inquiries were addressed to the individual making the inquiry rather than sent out on the net. This may take a bit of effort since you will have to address the message to the individual rather than simply reply to messages on the internet. This will lessen the clutter on the network. Dick Weiss From owner-mycology@net.bio.net Tue Sep 06 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!gatech!udel!news.sprintlink.net!news.world.net!news.teleport.com!usenet From: "Ralph D. Arnold" Newsgroups: bionet.mycology Subject: that unbelievable fungus tea?!? Date: Tue, 6 Sep 94 23:49:03 PST Organization: Teleport - Portland's Public Access (503) 220-1016 Lines: 105 Message-ID: <94739.rarnold@teleport.com> Reply-To: NNTP-Posting-Host: ip-c07.teleport.com X-Minuet-Version: Minuet1.0_Beta_16 X-POPMail-Charset: English To: 72253.1553@compuserve.com Cc: stamets1@aol.com Could you provide me with scientific info, such as the edibility of the mushroom itself (besides the yeasty broth) and price of cultures: The following is an extremely exerpted copy of posting by maschue@sdd.hp.com LATIN NAME?: But ~Pichia fermentans~, mentioned in the article, sounds like a reasonable guess. ~Pichia~, Hansen (1904), is one of 30 genera in the Saccharomycetaceae, the 4th family in the Endomycetales, a group of teleomorphic yeasts in the Ascomycotina. See Von Arx et al (Stud.mycol.14:1977) and others... We are talking about a "Panacea" Manchurian Mushroom that takes seven days to reproduce itself; it looks like a 6" diameter hot cake but is grayish in color... (Lists everything possible that could be good)... It was called by several different names: "Fungus japonicus", "Fungo-japon Kombucha", ~Pichia fermentans~, "Cembuya orientalis", "Combuchu", "Tschambucco", "Volga Spring", "mo-Gu", "Champignon de longue vie", "Teekwass", "Kwassan", and "Kargasok Tea". The kombucha fungus is built in membrane form and is a symbiosis of yeast cells and different bacteria. Among these bacteria are: - Bacterium xylinum, Bacterium gluconicum, Acetobacter ketogenum and pichia fermentans. (The latter is _not_ a bacterium!. Ed.) The kombucha fungus needs to live in a solution composed of black tea and sugar. In the right temperature they multiply constantly. They don't build spores as yeast normally does, but instead multiply by a process producing glucuronic acid, lactic acid, acetic acid and several vitamins. The yeast culture transforms the sugar and black tea into enzymesuseful for the body. The fermented mixture later contains not only these products, but also 0.5% alcohol. _Glucuronic acid_ is used in the body to build the improtant polyscharides such as: Hyaluronic acid which is vital for the connective tissues; Chondroitinsulfat acid which is the basic substance in our cartilage; Mykoitinsulfat acid which is for the mucous and for the virtreous (eye) and also Heparin and Lactic acid which is especially for our colon. =*=*= How to prepare kombucha tea =========================== Ingredients 1) One kombucha mushroom of about 3 inches in diameter. 2) Two litres (3 1/2 quarts) of fresh water (preferably natural mineral). 3) 15 grams (two tablespoons) biological green tea (Chinese or Japanese) 4) 200 grams (7 ozs.) refined white sugar (Some alternate recipe I've seen recommends light brown sugar.Ed.) 5) A stainless steel saucepan (do _not_ use aluminum) 6) A clean glass container with a wide mouth which will hold a 3" diameter mushroom and 2 litres of water 7) A piece of cheesecloth and a rubber band to close the jar A - The initial process - How to start the culture 1) Boil the water, remove the saucepan from the heat 2) Let it cool down for about 5 minutes 3) Add the tea and let it steep for about 15 minutes, then remove the tea bag. 4) Add the sugar and stir thoroughly 5) Let the tea cool down to about 90 degrees Fahrenheit 6) When making the first culture: Add two tablespoons of natural vinegar (which you omit when you already have mombucha batch made) 7) Pour the tea into the glass container and add the kombucha mushroom 8) Close the top with the cloth and rubber band. 9) Place the jar in a spot at a temperature of 80 degrees F. 10) After 10 to 14 days a new kombucha mushroom will have grown on the whole surface of the tea. 11) This kombucha tea should be strained, bottled, sealed with a clean lid and kept in the refrigerator. B - the subsequent process You make the new batch of tea with the new mushroom (which has grown on top of the old mushroom in the previous batch). The old mushroom can be used to fertilize plants, applied to wounds or used as cosmetic. When the new tea has cooled and been poured into the jar it should be mixed with about 8 oz. per quart of the previous (This sounds unreasonable, probably 'per litre'. Ed.) batch of kombucha tea. This favors the fermentationas a result of which the new kombucha tea will be ready in 8 to 10 days. Of course you have to put the kombucha mushroom in the cool tea and proceed as in step A above. Recommended dosage: 4 oz. every morning, on an empty stomach. Ralph D. Arnold P.O. Box 889 Oregon City, OR 97045 rarnold@teleport.com From owner-mycology@net.bio.net Tue Sep 06 23:00:00 1994 Path: biosci!cibnor.conacyt.mx!dianson From: dianson@cibnor.conacyt.mx ("David Ianson [BE]") Newsgroups: bionet.mycology Subject: Address Date: 7 Sep 1994 13:15:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Do any of you mycorrhizasts out there have an E-mail address for Dr. Joseph B. Morton, Department of Plant Pathology, Division of Forestry and Soil Sciences, West Virginia University, Morgantown, WV. I am trying to key out some unique species of VA mycorrhizal fungi and would like to contact him concerning wall structures, spore contents, and wall ornamentations. I would appreciate hearing from you. Dr. David C. Ianson Mycorrhizast and Research Microbiologist Department of Microbiology Division of Experimental Biology Centro de Investigaciones Biologicas La Paz, B.C.S. Mexico From owner-mycology@net.bio.net Tue Sep 06 23:00:00 1994 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!usc!howland.reston.ans.net!europa.eng.gtefsd.com!mozart.amil.jhu.edu!blaze.cs.jhu.edu!jhunix1.hcf.jhu.edu!NewsWatcher!user From: bains@welchlink.med.jhu.edu (user2) Newsgroups: bionet.mycology Subject: FAQ on growing mushrooms? Followup-To: bionet.mycology Date: 7 Sep 1994 17:35:21 GMT Organization: HCF - Johns Hopkins University, Balitmore, Maryland, USA Lines: 6 Message-ID: NNTP-Posting-Host: 128.220.71.152 Hi, Just wondering if there was a FAQ site for a novice that wants to learn about growing mushrooms. Thanks in Advance. Harshi From owner-mycology@net.bio.net Tue Sep 06 23:00:00 1994 Path: biosci!UMAIL.UMD.EDU!Jerome_J_MOTTA From: Jerome_J_MOTTA@UMAIL.UMD.EDU (jm102) Newsgroups: bionet.mycology Subject: Address Date: 7 Sep 1994 14:01:31 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 6 Sender: daemon@net.bio.net Distribution: world Message-ID: <9409072101.AA11815@umailsrv1.UMD.EDU> NNTP-Posting-Host: net.bio.net I understand an abstract appeared on the net concerning Pulse Field electrophoresis of chromosomes of Aspergillus sp. Could someone provide me with the E-mail address of the source? Many Thanks Jerry Motta From owner-mycology@net.bio.net Wed Sep 07 23:00:00 1994 Path: biosci!MOOSE.UVM.EDU!lvaillan From: lvaillan@MOOSE.UVM.EDU (Lisa Vaillancourt) Newsgroups: bionet.mycology Subject: re.address Date: 8 Sep 1994 12:17:46 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: <9409081917.AA78610@moose.uvm.edu> NNTP-Posting-Host: net.bio.net To Jerry Motta: I believe that the E-mail address you want is Klaas.swart@fungen.el.wau.nl Good Luck, Lisa Vaillancourt From owner-mycology@net.bio.net Wed Sep 07 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!swrinde!gatech!newsxfer.itd.umich.edu!nntp.cs.ubc.ca!news.UVic.CA!spruce.pfc.forestry.ca!PFC.Forestry.CA!RWINDER From: rwinder@PFC.Forestry.CA (Richard Winder) Subject: Re: Cultivation of Cantharellus cibarius, mykhorrhiza Message-ID: <1994Sep6.221240.28767@spruce.pfc.forestry.ca> Sender: news@spruce.pfc.forestry.ca Nntp-Posting-Host: pfc.pfc.forestry.ca Reply-To: rwinder@PFC.Forestry.CA Organization: Forestry Canada (Pacific Forestry Centre) References: ,<34avmh$n49@search01.news.aol.com> Date: Tue, 6 Sep 1994 22:12:40 GMT Lines: 50 In article <34avmh$n49@search01.news.aol.com>, stamets1@aol.com (Stamets 1) writes: >I remember a reference from a Sweedish or Norwegian mycologist who was >able >to get Cantharellus cibarius spores to germinate in the presence of a red >yeast named Rhodotorula glutinus (sp?). Cantharellus mycelium has been >slow for me. anyone else remember this reference? > >paul stamets Paul, I can't find the reference you mention, but you may be interested in these two (if you don't already have them): Straatsma, Gerben and Leo J.L.D. Van Griensven. 1986. Growth requirements of mycelial cultures of the mycorrhizal mushroom Cantharellus cibarius. Trans. Brit. Mycol. Soc. 87(1):135-141. Daniel, Eric, Sadhna Alström, and Anders Ternström. 1993. Psuedomonas fluorescens in association with fruit bodies of the ectomycorrhizal mushroom Cantharellus cibarius. Mycol. Res. 97(9):1148-1152. However, there *has* been work on induction of spore germination of the mycorrhizal mushroom Laccaria laccata through the use of Rhodotorula glutinis. This is described in: Fries, Nils. 1983. Spore germination, homing reaction, and intersterility groups in Lccaria laccata (Agaricales). Mycologia 75(2):221-227. I might as well unload the rest of my little folder here while I'm at it for the sake of general interest. There has been some other interesting work published on edible mycorrhizals that might be of some help: Pantidou, Maria E. 1961. Cultural studies of the boletaceae: Gyrodon merulioides and four species of boletinus. Can J. Bot. 39:1149-1162. McLaughlin, D. J. 1970. Environmental control of fruitbody development in Boletus rubinellus in axenic culture. Mycologia 62:307-331. Mischiati, Paola, and Anna Fontana. 1993. In vitro culture of Tuber magnatum mycelium isolated from mycorrhizas. Mycol. Res. 97(1):40-44. Hope these help. If anyone else has collected other references along these lines, I hope they will share them. -RSW RICHARD WINDER Title: Research Scientist Canadian Forest Service Phone: (604) 363-0773 Victoria, B.C. Internet: RWINDER@A1.PFC.Forestry.CA From owner-mycology@net.bio.net Wed Sep 07 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.cac.psu.edu!news.tc.cornell.edu!travelers.mail.cornell.edu!newstand.syr.edu!gamera.syr.edu!griffin From: griffin@gamera.syr.edu (David H. Griffin) Newsgroups: bionet.mycology Subject: Email replies Date: 8 Sep 1994 20:08:58 GMT Organization: Syracuse University, Syracuse, NY Lines: 18 Distribution: world Message-ID: <34nr0q$ano@newstand.syr.edu> NNTP-Posting-Host: gamera.syr.edu Newsgroups: bionet.mycology Subject: Email replies Summary: Followup-To: Distribution: world Organization: Syracuse University, Syracuse, NY Keywords: A recent posting suggested that replies to postings be made by email directly to the poster. The problem with this approach is that all discussion becomes private with little chance to interact and exchange ideas. What's the purpose of this group, anyway? David H. Griffin State University, College of Environmental Science & Forestry Syracuse NY griffin@mailbox.syr.edu From owner-mycology@net.bio.net Wed Sep 07 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.cac.psu.edu!news.tc.cornell.edu!travelers.mail.cornell.edu!newstand.syr.edu!gamera.syr.edu!griffin From: griffin@gamera.syr.edu (David H. Griffin) Newsgroups: bionet.mycology Subject: Email replies Date: 8 Sep 1994 19:49:06 GMT Organization: Syracuse University, Syracuse, NY Lines: 1 Distribution: world Message-ID: <34nprj$a89@newstand.syr.edu> NNTP-Posting-Host: gamera.syr.edu From owner-mycology@net.bio.net Wed Sep 07 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!news.uoregon.edu!gaia.ucs.orst.edu!news.CSOS.ORST.EDU!microbe From: microbe@CSOS.ORST.EDU (Steven Carpenter) Newsgroups: bionet.mycology Subject: Re: Cultivation of Cantharellus cibarius, mykhorrhiza Date: 7 Sep 1994 19:02:02 GMT Organization: CS Outreach Services, Oregon State University, Corvallis, OR, USA Lines: 16 Message-ID: <34l2nb$g6@jadzia.CSOS.ORST.EDU> References: <34avmh$n49@search01.news.aol.com> NNTP-Posting-Host: csos.orst.edu X-Newsreader: TIN [version 1.2 PL2] Dr. Fries from Sweden performed the experiment with yeast. You may also find that a number of non-fungal microbes are necessary for the growth of mycelium in vitro. -Steve Carpenter Abbey Lane Laboratories Stamets 1 (stamets1@aol.com) wrote: : I remember a reference from a Sweedish or Norwegian mycologist who was : able : to get Cantharellus cibarius spores to germinate in the presence of a red : yeast named Rhodotorula glutinus (sp?). Cantharellus mycelium has been : slow for me. anyone else remember this reference? : paul stamets From owner-mycology@net.bio.net Thu Sep 08 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!gatech!news-feed-1.peachnet.edu!news.duke.edu!solaris.cc.vt.edu!uunet!newstf01.cr1.aol.com!search01.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: Re: Need help!- first time mushroom grower Date: 8 Sep 1994 21:40:04 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 14 Sender: news@search01.news.aol.com Message-ID: <34oedk$emv@search01.news.aol.com> References: NNTP-Posting-Host: search01.news.aol.com In article , TennentG@micr.unp.ac.za (Geoff.Tennant) writes: yikes! i stand by the casing formulas in The Mushroom Cultivator and in Growing Gourmet and Medicinal Mushrooms. (this is an easy way for me to avoid directly answering your questions!!!) whatever works for you is the best! paul From owner-mycology@net.bio.net Thu Sep 08 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!gatech!news-feed-1.peachnet.edu!news.duke.edu!solaris.cc.vt.edu!uunet!newstf01.cr1.aol.com!search01.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: Re: Fungus tea Date: 8 Sep 1994 21:34:02 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 22 Sender: news@search01.news.aol.com Message-ID: <34oe2a$ejs@search01.news.aol.com> References: NNTP-Posting-Host: search01.news.aol.com In article , TennentG@micr.unp.ac.za (Geoff.Tennant) writes: A woman died in Florida about a month ago from ingesting a contamiant (presumably) or perhaps died of allergic shock. I must have 7 little hand-outs or booklets, poorly photocopied instructions, the whole gamut, describing the virtues of Kambucha. I do think that if you have been cut, in the woods, the gelatinous sheath would inhibit bacterial infection. I have heard antedoctal reports to this effect. I still have a culture, as much as I try to abuse/kill it. Right now, it has been in full sun for 3 months and still is viable. thanks for the communication. paul From owner-mycology@net.bio.net Thu Sep 08 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!gatech!newsxfer.itd.umich.edu!uunet!newstf01.cr1.aol.com!search01.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: Re: Cultivation of Cantharellus cibarius, mykhorrhiza Date: 8 Sep 1994 21:28:01 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 8 Sender: news@search01.news.aol.com Message-ID: <34odn1$efb@search01.news.aol.com> References: <1994Sep6.221240.28767@spruce.pfc.forestry.ca> NNTP-Posting-Host: search01.news.aol.com In article <1994Sep6.221240.28767@spruce.pfc.forestry.ca>, rwinder@PFC.Forestry.CA (Richard Winder) writes: thanks for the info. I think Nils Fries is the one who published the spore germination w/R. glutinus article. check out his bibliography in the article you sighted. I should think it would be there. paul From owner-mycology@net.bio.net Thu Sep 08 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!gatech!news-feed-1.peachnet.edu!news.duke.edu!solaris.cc.vt.edu!uunet!newstf01.cr1.aol.com!search01.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: Re: Fungus tea Date: 8 Sep 1994 21:36:09 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 15 Sender: news@search01.news.aol.com Message-ID: <34oe69$el5@search01.news.aol.com> References: <9409071433.AA00982@uclachem.chem.ucla.edu> NNTP-Posting-Host: search01.news.aol.com In article <9409071433.AA00982@uclachem.chem.ucla.edu>, rlw@CHEM.UCLA.EDU (Richard Weiss) writes: i am sending direct response to the individual addressing me. I do not understand how the communication can be sent more restrictively. please be specific and give examples...or don't read the letters you don't want to. Nobody forcing you. paul stamets From owner-mycology@net.bio.net Thu Sep 08 23:00:00 1994 Path: biosci!U.WASHINGTON.EDU!todell From: todell@U.WASHINGTON.EDU ("Thomas O'Dell") Newsgroups: bionet.mycology Subject: (none) Date: 9 Sep 1994 13:07:02 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 42 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net This message is in MIME format. The first part should be readable text, while the remaining parts are likely unreadable without MIME-aware tools. ---1936831475-2078917053-779141217:#69461 Content-Type: TEXT/PLAIN; charset=US-ASCII attached is the annoucement for a forthcoming job. I hope to hire someone in early october. ---1936831475-2078917053-779141217:#69461 Content-Type: TEXT/PLAIN; charset=US-ASCII; name="ad.txt" Content-Transfer-Encoding: BASE64 Content-ID: Content-Description: VGVjaG5pY2FuIE5lZWRlZA0KRHV0aWVzOg0KQXNzaXN0IHdpdGggZmllbGQg c2FtcGxpbmcgb2Ygc29pbCwgZnVuZ2kgYW5kIHJvb3RzLCBmaWVsZCBkYXRh IGNvbGxlY3Rpb24gYXMgcmVxdWlyZWQuDQpQZXJmb3JtIEROQSBleHRyYWN0 aW9ucyBhbmQgYW5hbHlzZXMgZm9yIGZ1bmd1cyBhbmQgcGxhbnQgc3BlY2ll cyBpZGVudGlmaWNhdGlvbnMuDQoNCk9wZXJhdGUgYSB2YXJpZXR5IG9mIGxh Ym9yYXRveSBpbnN0cnVtZW50cyBpbmNsdWRpbmcgcEggbWV0ZXJzLCBjZW50 cmlmdWdlcywgZWxlY3Ryb3Bob3Jlc2lzIGFwcGFyYXR1cy4gUHJlcGFyZSBy ZWFnZW50czsgcGVyZm9ybSBkYXRhIGVudHJ5OyBwZXJmb3JtIHBob3RvbWlj cm9ncmFwaHkgYW5kIG1pY3Jvc2NvcGljIG1vcnBob2xvZ2ljYWwgZGVzY3Jp cHRpb25zIG9mIGVjdG9teWNvcnJoaXphZTsgd2FzaGluZyByb290IHNhbXBs ZXM7IG90aGVyIHJlbGF0ZWQgZHV0aWVzIGFzIHJlcXVpcmVkLiANCktlZXAg ZGV0YWlsZWQgcmVjb3JkcyBvZiBzdHVkaWVzIGNvbmR1Y3RlZC4gUmVhZCBz Y2llbnRpZmljIGxpdGVyZWF0dXJlIHBlcnRpbmFudCB0byB0aGUgcmVzZWFy Y2ggYXJlYS4NCg0KU3BlY2lhbCBza2lsbHMvZWR1Y2F0aW9uDQoNCk11c3Qg YmUgd2lsbGluZyB0byB3b3JrIGluIHJlbW90ZSBmaWVsZCBzaXRlcywgbG9u ZyBob3VycyBpbiBwb29yIHdlYXRoZXI7IG92ZXJuaWdodCB0cmF2ZWwgcmVx dWlyZWQuDQpFeHBlcmlhbmNlIGluIGVjb2xvZ2ljYWwgZmllbGQgc2FtcGxp bmcsIGZ1bmd1cyBpZGVudGlmaWNhdGlvbiBhbmQgZGVzY3JpcHRpb24sIFBD UiBiYXNlZCBETkEgYW5hbHlzZXMgYXJlIGRlc2lyZWQuIFByZWZlciBzb21l b25lIHdpdGggYW4gaW50ZXJlc3QgaW4gbXljb2xvZ3kvbXljb2Vjb2xvZ3ks IGZpZWxkIGV4cGVyaWVuY2UgaW4gV2VzdGVybiBXYXNoaW5ndG9uIGVjb3N5 c3RlbXMsIFBDUiBETkEgYW1wbGlmaWNhdGlvbiwgYW5kIFBDUiBETkEgc2Vx dWVuY2luZy4NCg0KRm9yIE1vcmUgSW5mb3JtYXRpb24gYW5kIGFwcGxpY2F0 aW9uIG1hdGVyaWFscyBjb250YWN0Og0KVC4gTydEZWxsLCBEZXBhcnRtZW50 IG9mIEJvdGFueSwgS0ItMTUsIFVuaXZlcnNpdHkgb2YgV2FzaGluZ3Rvbiwg U2VhdHRsZSwgV0EgOTgxOTUNCnRvZGVsbEB1Lndhc2hpbmd0b24uZWR1DQo= ---1936831475-2078917053-779141217:#69461-- From owner-mycology@net.bio.net Thu Sep 08 23:00:00 1994 Path: biosci!U.WASHINGTON.EDU!todell From: todell@U.WASHINGTON.EDU ("Thomas O'Dell") Newsgroups: bionet.mycology Subject: job announcement, queries Date: 9 Sep 1994 16:22:39 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net sorry, the readable version lacks an address reply to me by email or T. O'Dell, Dept. of Botany, KB-15, University of Washington, Seattle, WA 98195 for more info thanks for your patience From owner-mycology@net.bio.net Thu Sep 08 23:00:00 1994 Path: biosci!U.WASHINGTON.EDU!todell From: todell@U.WASHINGTON.EDU ("Thomas O'Dell") Newsgroups: bionet.mycology Subject: job announcement Date: 9 Sep 1994 15:45:44 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 47 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Molecular Ecologist Technician Starting: ? about 1 Oct. 1994 Duties: Assist with field sampling of soil, fungi and roots, field data collection as required. Perform DNA extractions and analyses for fungus and plant species identifications. Operate a variety of laboratoy instruments including pH meters, centrifuges, electrophoresis apparatus. Prepare reagents; perform data entry; perform photomicrography and microscopic morphological descriptions of ectomycorrhizae; washing root samples; other related duties as required. Keep detailed records of studies conducted. Read scientific litereature pertinant to the research area. Special skills/education Must be willing to work in remote field sites, long hours in poor weather; overnight travel required. Experiance in ecological field sampling, fungus identification and description, PCR based DNA analyses are desired. Prefer someone with an interest in mycology/mycoecology, field experience in Western Washington ecosystems, PCR DNA amplification, and PCR DNA sequencing. Abstract of funded proposal follows. A Comparison of Sporocarp and Mycorrhiza Dominance of Ectomycorrhizal Fungi At one site with high ectomycorrhizal fugus species richness and one site with low species richness we will attempt to answer the following questions: 1) Are the ectomycorrhizal fungus species that produce the most sporocarps colonizing the most root tips (i.e., forming the most mycorrhizae)? 2) Is sporocarp biomass or frequency (number of plots where it is detected) better correlated with mycorrhizae abundance of important fungus species? 3) Do fungus species vary in their prefered substrate (organic vs. mineral soil) for mycorrhizae formation? 4) Do the fungus species forming the most mycorrhizae vary by season? 5) Does peak sporocarp production occur during the season of peak mycorrhizae abundance? We will determine which species of ectomycorrhizal fungi produce sporocarps most frequently and abundantly in each stand by collecting sporocarps for biomass determination from systematically placed plots of known area. The most numerous morphological types of ectomycorrhizae in each stand will be determined from soil cores. Dominant mycorrhizal types will be compared with species producing the most frequent and abundant sporocarps using RFLPs of fungal ITS DNA amplified from sporocarps and ectomycorrhizae. From owner-mycology@net.bio.net Thu Sep 08 23:00:00 1994 Path: biosci!daresbury!bioftp.unibas.ch!citi2.fr!jussieu.fr!sxpo.fdn.org!techlink!lionel Message-ID: <1867697947.21267094@techlink.techlink.fr> Date: 09 Sep 1994 20:26:18 GMT From: lionel@techlink.fr (Lionel Benhamou) Organization: TechnoLink, FirstClass Server Reply-To: lionel@techlink.fr Subject: Re: Status of MYCONET Distribution: world Newsgroups: bionet.mycology Lines: 16 > I've tried to subscribe to a couple of lists offered throught MYCONET a couple of > times now but have not gotten any response. Does anyone have > any info on them? Are they still up and running? I subscribed 10 days ago and Im still waiting too. Any news ? bientt. Lionel ***lionel@techlink.fr*** ========================================================================== TechnoLink - FC Server - France | Renseignements : info@techlink.fr (33-1) 49 83 86 49 | Fax :(33-1) 49 83 81 17 ========================================================================== From owner-mycology@net.bio.net Thu Sep 08 23:00:00 1994 Path: biosci!BULL.CC.USM.EDU!GLEN_SHEARER From: GLEN_SHEARER@BULL.CC.USM.EDU (GLEN SHEARER) Newsgroups: bionet.mycology Subject: Important info. Date: 9 Sep 1994 15:10:36 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: <940909.17094188.044802@USM.CP6> NNTP-Posting-Host: net.bio.net The following critically valuable information is encoded in the three common formats (Epicurean pictograms, Machiavellian hieroglyphs and Minoan angiograms) for your convenience: fiGfh%*78%$/'[()20=fG-bR4%c!#@fl90Prx""cT5Fg: 2b-1aI+??74(0)Ynx2Njfl40(ln )nnf 2$%^lnClnXt 10FTiOppr-(";?. From owner-mycology@net.bio.net Thu Sep 08 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!swiss.ans.net!malgudi.oar.net!chemabs!usenet From: jac55@cas.org () Subject: Re: Abstract:how to submit? Message-ID: <1994Sep9.201306.11604@chemabs.uucp> Sender: usenet@chemabs.uucp Reply-To: jac55@cas.org Organization: Chemical Abstracts Service References: <34h634$jh3@hippo.shef.ac.uk> Date: Fri, 9 Sep 1994 20:13:06 GMT Lines: 32 In article jh3@hippo.shef.ac.uk, mb1gt@sunc.sheffield.ac.uk (G Turner) writes: >Is it possible to send a file to bionet.mycology >via the reader (similar to posting messages like this one) >without subscribing. If so, which command? If not, what >is the email address of bionet? >Typing out an abstract using this system is too laborious. >Geoff Turner >G.Turner@sheffield.ac.uk If you're using a UNIX box then it's fairly easy. With an older newsreader like "rn" you start your post as normal and then you copy the file into the post using the "cat" command, i.e. cat . If you have an X-window machine, or any other graphical user interface machine using xrn, or xvnews or something comparable, then all the commands you need should be available through buttons or pull down menus. Your system administrator should be able to tell what commands to use with your machine. Alec This either a forgery or a very clever original. **************************************************************** *Alec Chambers *My employers and I do not speak * *(jac55@cas.org) *for one another, this is a most * *Associate Editor *agreeable arrangement. * *Chemical Abstracts Service * * *P.O.Box 3012 * * *Columbus, OH 43210 * Phone:(614)-447-3600 ext. 3992. * **************************************************************** From owner-mycology@net.bio.net Thu Sep 08 23:00:00 1994 Path: biosci!U.WASHINGTON.EDU!todell From: todell@U.WASHINGTON.EDU ("Thomas O'Dell") Newsgroups: bionet.mycology Subject: email adress Date: 9 Sep 1994 13:08:16 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net does anyone have an email adress for R. Agerer? or know how to obtain his Colour Atlas of Ectomycorrhizae? thx From owner-mycology@net.bio.net Thu Sep 08 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!europa.eng.gtefsd.com!newsxfer.itd.umich.edu!uunet!news.delphi.com!usenet From: Agaricus Newsgroups: bionet.mycology Subject: Re: Cultivation of Cantharellus cibarius, mykhorrhiza Date: Thu, 8 Sep 94 22:30:05 -0500 Organization: Delphi (info@delphi.com email, 800-695-4005 voice) Lines: 11 Message-ID: References: NNTP-Posting-Host: bos1g.delphi.com X-To: Bjorn Ness Bjorn: The Cantharellus cibarius culture is obtainable through the Department of Plant Pathol;ogy College of Agricultural Sciences at Penn State University. The address is 210 Buckhout Laboratory: University Park, PA16802-4507U.S.A. $45.00/tube plus $5.00 S.&H. Prepaid Order must be accompanied by a draft on a U.S.bank or an international money order payable to The Pennsylvania State University. Culture # is 359 I mean 560, sorry. Paul J. Wuest is the Curator -- many others are also available. From owner-mycology@net.bio.net Thu Sep 08 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!vixen.cso.uiuc.edu!news.uoregon.edu!gaia.ucs.orst.edu!news.CSOS.ORST.EDU!microbe From: microbe@CSOS.ORST.EDU (Steven Carpenter) Newsgroups: bionet.mycology Subject: Re: Fungus tea Date: 8 Sep 1994 13:30:40 GMT Organization: CS Outreach Services, Oregon State University, Corvallis, OR, USA Lines: 27 Message-ID: <34n3m0$ija@jadzia.CSOS.ORST.EDU> References: <34ihft$ml0@mserv1.dl.ac.uk> <34ir7t$nk0@search01.news.aol.com> NNTP-Posting-Host: csos.orst.edu X-Newsreader: TIN [version 1.2 PL2] Don, Try posting to "alt.folklore.herbs" - there was a good response to a question on this substance earlier this year. I will check with the Asia Pacific International Corporation for more leads. -Steve Carpenter Abbey Lane Laboratories Stamets 1 (stamets1@aol.com) wrote: : In article <34ihft$ml0@mserv1.dl.ac.uk>, Don Reynolds : writes: : the fungus tea you mention might be referring to one called the : "Manchurian : Mushroom" or Chambucha, Kambuska, etc.... : I have grown it and have lots of information on it. : It is very popular with the New Agers who : would rather drink a substance of unknown : identity than to learn that it may kill you. : seriously. give me your address and i : will send you an info sheet on it. : paul stamets From owner-mycology@net.bio.net Thu Sep 08 23:00:00 1994 Path: biosci!EFN.ORG!mlandman From: mlandman@EFN.ORG (Marshall Landman) Newsgroups: bionet.mycology Subject: Status of MYCONET Date: 8 Sep 1994 20:58:19 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I've tried to subscribe to a couple of lists offered throught MYCONET a couple of times now but have not gotten any response. Does anyone have any info on them? Are they still up and running? The rain that has fallen here in Eugene should help early flushes come forth. Think I'll go out looking this weekend. Marshall Landman mlandman@efn.org From owner-mycology@net.bio.net Fri Sep 09 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!europa.eng.gtefsd.com!news.uoregon.edu!usenet.coe.montana.edu!mtvms2.mtech.edu!DVANDERVOORT From: dvandervoort@mtvms2.mtech.edu Newsgroups: bionet.mycology Subject: Paecilomyces production Date: 9 Sep 1994 14:29:48 GMT Organization: Montana Tech Lines: 15 Message-ID: <34prgs$l1d@pdq.coe.montana.edu> Reply-To: dvandervoort@mtvms2.mtech.edu NNTP-Posting-Host: mtvms2.mtech.edu My wife works in large-scale production of Paecilomyces fumosoroseus. She's not hooked to the net so she asked me to post these questions. What is viability? How best to measure viability? What is lost when viability lost (enzyme systems)? Same for virulence... I hope that makes sense. I wrote the questions down verbatim. If you can answer these questions or know someone who can please respond. This is my first posting. I'm a geologist so I'm not worried about embarassing myself in fornt of a bunch of mycologists. Sorry, no signature (yet). From owner-mycology@net.bio.net Fri Sep 09 23:00:00 1994 Path: biosci!GAR.UNION.EDU!SALVOJ From: SALVOJ@GAR.UNION.EDU ("Jill Salvo") Newsgroups: bionet.mycology Subject: ultrastructure of fungal mitochondria Date: 10 Sep 1994 07:00:05 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <199409101400.GAA13318@net.bio.net> NNTP-Posting-Host: net.bio.net We have recently been sifting unsuccessfully through the literature for any information on the structure of fungal mitchondria, in particular Neurospora, Aspergillus and Podospora. Does anyone know of any studies using electron microsopy or other techniques to observe the mitochondria during the life cycle of any of these? Thanks in advance. Jill Salvo salvoj@gar.union.edu From owner-mycology@net.bio.net Sat Sep 10 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!noc.near.net!saturn.caps.maine.edu!news.ycc.yale.edu!llilag From: llilag@minerva.cis.yale.edu (Larry Ilag) Newsgroups: bionet.mycology Subject: fungal pathogens Date: 11 Sep 1994 18:00:54 GMT Organization: Yale University Lines: 6 Message-ID: <34vgkm$sln@news.ycc.yale.edu> NNTP-Posting-Host: minerva.cis.yale.edu X-Newsreader: TIN [version 1.2 PL2] I would appreciate it very much if someone could provide me a sampling (a list) of fungal pathogens that exhibit race (cultivar) specificity and are amenable to genetic analysis. I would also be grateful for references to approriate literature. Thank you in advance. Larry Ilag, PhD From owner-mycology@net.bio.net Sat Sep 10 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!pipex!sunic!trane.uninett.no!eunet.no!nuug!EU.net!uunet!newstf01.cr1.aol.com!search01.news.aol.com!not-for-mail From: schoolcamp@aol.com (Schoolcamp) Newsgroups: bionet.mycology Subject: Re: Mushroom forays Date: 11 Sep 1994 10:12:04 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 19 Sender: news@search01.news.aol.com Message-ID: <34v37k$7tp@search01.news.aol.com> References: <32cdgf$3ph@guru.med.cornell.edu> NNTP-Posting-Host: search01.news.aol.com In article <32cdgf$3ph@guru.med.cornell.edu>, fgrun@med.cornell.edu (fgrun) speaks of forays north of Albany. I am in Lake Placid and went out yesterday at the recommendation of a hiker friend who said he had seen birch trees covered with something that looked as though it would be delicious.I went out with hopes of oysters or chickens and found instead somethig which I do not know: beautiful massed clumps of s brown topped, dry buffgilled species, veiled, ringed, gills fairly crowded, slightly decurrent. I filled my day pack and have spent the morning with my books and without a satisfactory outcome. I am making a spore print right now. These were growing from the base and about three feet up a birch tree, probably dead though I failed to confirm that. Also found Pholiotas in abundance and another smaller veiled species also clumped on birch,yellow-brown, brown gills. Both of these taste sweet and delicious but I am nervous about going any further without more certain identification. Any help would be appreciated. Frank Wallace at North Country School - Camp Treetops From owner-mycology@net.bio.net Sat Sep 10 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!agate!spool.mu.edu!umn.edu!dialup-3-156.gw.umn.edu!sjacobs From: sjacobs@maroon.tc.umn.edu (Steve Jacobson) Subject: HELP! Strange Mold Message-ID: Sender: news@news.cis.umn.edu (Usenet News Administration) Nntp-Posting-Host: dialup-3-156.gw.umn.edu Organization: University of Minnesota, Twin Cities X-Newsreader: Trumpet for Windows [Version 1.0 Rev B final beta #1] Date: Sun, 11 Sep 1994 02:04:41 GMT Lines: 31 Could anyone out there help me. Someone from the bionet.general newsgroup suggested I post this here. My cousin has a strange mold/organism growing throughout her house (on the end of Long Island, NY). It started with a leak in a gutter that eventually got into the house (the house was cool and humid). It incubated over the summer and when the weather became humid, it started breeding rapidly. The humidity rapidly built up in the house early in the summer and everything became damp. The house never fully dried out. The mold starts out with a pinkish color with a round shape (like little droplets). The shape then will change to become irregular. The mold then starts to grow and get darker (orange or brown). It takes on all kinds of shapes, but its favorite is round-like ameoba or protoplasm. The mold grows very large and spreads fast. It covers everything, especially porus items, like paper, wood, and fabric (also pottery, paint, and wood floors) When the mold gets ahold and spreads, it turns darker. Clorox does not seem to kill it-it sometimes lightens it a little (maybe one out of 10 cases). There is also a version of this mold that is gray or black. Does anyone know what this stuff is and HOW TO KILL IT. Thanx in advance. ----------------------------------------------- Rgds Steve Jacobson sjacobs@maroon.tc.umn.edu From owner-mycology@net.bio.net Sat Sep 10 23:00:00 1994 Path: biosci!VENUS.TAMU.EDU!npk3325 From: npk3325@VENUS.TAMU.EDU (Nancy Keller) Newsgroups: bionet.mycology Subject: teaching thanks Date: 11 Sep 1994 10:22:37 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: <199409111722.KAA20953@net.bio.net> NNTP-Posting-Host: net.bio.net I appreciate all of you who gave me some ideas on teaching labs. & if you've got any other neat ideas, please pass by. I'd be happy to share those labs that were & weren't successful. Nancy P. Keller Dept. Plant Pathology and Microbiology Texas A&M University College Station, TX 77843-2132 409-845-0963 P 409-845-8463 F npk3325@venus.tamu.edu From owner-mycology@net.bio.net Sun Sep 11 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!spool.mu.edu!mnemosyne.cs.du.edu!nyx.cs.du.edu!not-for-mail From: rwood@nyx.cs.du.edu (robert wood) Newsgroups: bionet.mycology Subject: Re: Fungus tea Date: 12 Sep 1994 07:53:52 -0600 Organization: University of Denver, Math/CS Dept. Lines: 24 Message-ID: <351mhg$s02@nyx.cs.du.edu> References: <9409071433.AA00982@uclachem.chem.ucla.edu> <1994Sep9.162023.14248@spruce.pfc.forestry.ca> NNTP-Posting-Host: nyx.cs.du.edu rwinder@PFC.Forestry.CA (Richard Winder) writes: >In article <9409071433.AA00982@uclachem.chem.ucla.edu>, >rlw@CHEM.UCLA.EDU (Richard Weiss) writes: >>It would benefit all subscribers to mycology@net.bio.net if responses to >>individual inquiries were addressed to the individual making the inquiry >>rather than sent out on the net. This may take a bit of effort since you >>will have to address the message to the individual rather than simply reply >>to messages on the internet. This will lessen the clutter on the network. >> >>Dick Weiss >Clutter? I was rather enjoying the discussion... -RSW > RICHARD WINDER Title: Research Scientist > Canadian Forest Service Phone: (604) 363-0773 > Victoria, B.C. Internet: RWINDER@A1.PFC.Forestry.CA I Agree!! One will never be able to learn of information available if one does not know it exists... From owner-mycology@net.bio.net Sun Sep 11 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!agate!overload.lbl.gov!ames!elroy.jpl.nasa.gov!usc!nic-nac.CSU.net!charnel.ecst.csuchico.edu!psgrain!nntp.cs.ubc.ca!news.UVic.CA!spruce.pfc.forestry.ca!PFC.Forestry.CA!RWINDER From: rwinder@PFC.Forestry.CA (Richard Winder) Subject: Re: Fungus tea Message-ID: <1994Sep9.162023.14248@spruce.pfc.forestry.ca> Sender: news@spruce.pfc.forestry.ca Nntp-Posting-Host: pfc.pfc.forestry.ca Reply-To: rwinder@PFC.Forestry.CA Organization: Forestry Canada (Pacific Forestry Centre) References: <9409071433.AA00982@uclachem.chem.ucla.edu> Date: Fri, 9 Sep 1994 16:20:23 GMT Lines: 16 In article <9409071433.AA00982@uclachem.chem.ucla.edu>, rlw@CHEM.UCLA.EDU (Richard Weiss) writes: >It would benefit all subscribers to mycology@net.bio.net if responses to >individual inquiries were addressed to the individual making the inquiry >rather than sent out on the net. This may take a bit of effort since you >will have to address the message to the individual rather than simply reply >to messages on the internet. This will lessen the clutter on the network. > >Dick Weiss Clutter? I was rather enjoying the discussion... -RSW RICHARD WINDER Title: Research Scientist Canadian Forest Service Phone: (604) 363-0773 Victoria, B.C. Internet: RWINDER@A1.PFC.Forestry.CA From owner-mycology@net.bio.net Sun Sep 11 23:00:00 1994 Path: biosci!UMAIL.UMD.EDU!David_C_STRANEY From: David_C_STRANEY@UMAIL.UMD.EDU (ds106) Newsgroups: bionet.mycology Subject: abstract: Homologous in vitro transcription Date: 12 Sep 1994 10:49:46 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 62 Sender: daemon@net.bio.net Distribution: world Message-ID: <9409121749.AA12349@umailsrv1.UMD.EDU> NNTP-Posting-Host: net.bio.net 2 abstracts relating to reconstitution of regulated transcription in homologous mycelial extracts abstract: in press, Current Genetics IN VITRO TRANSCRIPTION FROM THE NECTRIA HAEMATOCOCCA PDA1 PROMOTER IN HOMOLOGOUS EXTRACT REFLECTS IN VIVO PISATIN- RESPONSIVE REGULATION Yijun Ruan and David C. Straney Department of Botany and Maryland Agricultural Experiment Station, University of Maryland, College Park Maryland 20742 The PDA1 gene of Nectria haematococca MP VI (anamorph: Fusarium solani) encodes pisatin demethylase. This enzyme detoxifies the isoflavanoid phytoalexin pisatin, produced by the plant on which this fungus is pathogenic. Expression of pisatin demethylase activity is induced in mycelium by pretreatment with pisatin. We have developed a homologous in vitro transcription system which accurately initiates transcription from the PDA1 promoter. Transcription levels in vitro reflect the same pisatin-responsive stimulation as measured for PDA1 mRNA in vivo, and are dependent upon sequences in the 5' upstream region of PDA1. Pisatin-responsive transcription from the PDA1 promoter indicates that initiation of transcription is a major regulatory step in pisatin induction of pisatin demethylase expression. Another related abstract: Gene, in press A PCR-BASED CONSTRUCTION OF PROMOTER/G-FREE TEMPLATES FOR IN VITRO TRANSCRIPTION ANALYSIS ALLOWS SELECTION OF PLASMIDS WITH OPTIMAL ACTIVITY IN HOMOLOGOUS EXTRACTS Yijun Ruan and David C. Straney Department of Botany and Maryland Agricultural Experiment Station, University of Maryland, College Park, MD 20742, USA In vitro transcription has been used for dissecting transcriptional controls in many eukaryotic systems. One modification which greatly reduces background non-specific transcription is the placement of a guanosine-free (G-free) region of DNA immediately downstream of a promoter [Sawadogo and Roeder, Proc. Natl. Acad. Sci. USA 82 (1985) 4394-4398]; transcription in the presence of RNase T1 and 3' O-Me-GTP eliminates non-specific transcripts, and produces the G-free transcripts initiated at the promoter. Restriction site-based fusion of a G-free cassette downstream of promoters is complicated by the requirement for G nucleotides to be excluded from the coding strand downstream of the transcription start points. We present an approach to add a G-free template onto a eukaryotic promoter by combining PCR-based termini construction and terminal deoxynucleotidyl transferase extension. The pisatin demethylase promoter (PDA1p) of the filamentous fungus Nectria haematococca, was used as the test promoter. Three PDA1p/G-free constructs were tested in heterologous Drosophila melanogaster and HeLa and homologous N. haematococca transcription extracts. Each extract produced a PDA1p-specific transcript from each construct, but the relative level of transcription between constructs varied, particularly in the homologous extract. Since the choice of G-free sequence influences transcription differently among systems, this method for producing multiple G-free constructs should be useful for constructing and selecting optimal promoter/G-free templates for in vitro transcription in other homologous systems. From owner-mycology@net.bio.net Sun Sep 11 23:00:00 1994 Path: biosci!ADMIN.OGI.EDU!msachs From: msachs@ADMIN.OGI.EDU (Matthew Sachs) Newsgroups: bionet.mycology Subject: Re: ultrastructure of fungal mitochondria Date: 12 Sep 1994 08:03:41 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <9409121503.AA24607@admin.ogi.edu> NNTP-Posting-Host: net.bio.net >We have recently been sifting unsuccessfully through the literature for any >information on the structure of fungal mitchondria, in particular Neurospora, >Aspergillus and Podospora. Does anyone know of any studies using electron >microsopy or other techniques to observe the mitochondria during the life >cycle of any of these? Thanks in advance. > Jill Salvo > salvoj@gar.union.edu These are some older literature references indexed under the rubric "mitochondria-morphology-electron microscopy" from the Neurospora Bibliography and Index, 1965. Luck, D.J.L., Genesis of mitochondria in Neurospora crassa. PNAS 49:233-240, 1963 Shatkin, A.J. and Tatum, E.L., Electron microscopy of Neurospora crassa mycelia. J. Biophys. Biochem. Cytol. 6:423-426, 1959 Shatkin, A.J. and Tatum, E.L., The relationshop of m-inositol to morphology in Neurospora crassa. Am. J. Botany 48:760-771, 1961 Zalokar, M., Electron microscopy of centrifuged hyphae of Neurospora. J. Biophys. Biochem. Cytol. 9:609-617, 1961 ----------------------------------------------------------- Matthew Sachs Department of Chemistry, Biochemistry and Molecular Biology Oregon Graduate Institute of Science and Technology 20000 NW Walker Road P.O. Box 91000 Portland, OR 97291-1000 503 690-1487 Phone 503 690-1464 Fax msachs@admin.ogi.edu From owner-mycology@net.bio.net Sun Sep 11 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!bcm!cs.utexas.edu!uunet!panix!zip.eecs.umich.edu!umn.edu!news From: brambl@graz.cbs.umn.edu (Robert Brambl) Subject: Re: teaching thanks Message-ID: Sender: news@news.cis.umn.edu (Usenet News Administration) Nntp-Posting-Host: graz.cbs.umn.edu Reply-To: brambl@molbio.cbs.umn.edu (Robert Brambl) Organization: University of Minnesota, Twin Cities References: <199409111722.KAA20953@net.bio.net> Date: Mon, 12 Sep 1994 18:35:45 GMT Lines: 39 In article <199409111722.KAA20953@net.bio.net> npk3325@VENUS.TAMU.EDU (Nancy Keller) writes: > I appreciate all of you who gave me some ideas on teaching labs. & if > you've got any other neat ideas, please pass by. I'd be happy to share > those labs that were & weren't successful. > Nancy P. Keller Several years ago Salomon Bartnicki-Garcia (bart@ucrac1.ucr.edu) published (I think in the ASM News) a lab demonstration of dimorphism in Mucor that is easy and nifty. He cooled agar almost to the point of solidification, mixed in Mucor spores, and poured the suspension into a plastic beaker. Later, this beaker was cut in half to reveal a dimorphic cell gradient from top to bottom (reflecting, presumably, an oxygen gradient) that could be seen with a low-power microscope. Anyone else tried this? Another easy demonstration that also can be used in an experimental sense is an assay of pectinases produced by Rhizopus. This fungus, when grown as a floating mat on a liquid medium (consisting of sweet potato broth) produces a powerful pectinase. (Be certain to get a producing isolate of Rhizopus directly from the surface of any sweet potato.) Then, the enzymology begins. The enzyme activity assayed directly in the filtered culture medium or precipitated with ammonium sulfate for concentration. A simple acoustic assay (quantitative: time-to-endpoint) for enzyme activity is to prepare uniform thin disks of carrot root that are perforated in the centers. One hook placed through the perforated disk anchors the enzyme substrate (the carrot disk) to the bottom of the incubation tube, and another hook is tied to a string that hangs out of the incubation tube with a small weight on the other end. Digestion of the plant tissue causes the weight to drop noisily into a metal pan on the floor. (I know, I know. This sounds comical, but it works in its primative way.). One can set up several assays at once to record enzyme protein concentration and denaturation effects, pH, temperature, culture production curves, etc.). If nothing else, it teaches a new respect for the discoverers and suppliers and costs of restriction enzymes. Best wishes. Bob From owner-mycology@net.bio.net Mon Sep 12 23:00:00 1994 Path: biosci!HUGO.FSUFAY.EDU!dhaas From: dhaas@HUGO.FSUFAY.EDU Newsgroups: bionet.mycology Subject: Growing morel mushrooms Date: 13 Sep 1994 06:54:43 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <94091309470255@hugo.fsufay.edu> NNTP-Posting-Host: net.bio.net Hello mycos Does anyone know anything about Gourmet Mushrooms, Inc. They claim that with a Morel Habitat Kit that you can grow Morels in your back yard. I thought this couldn't be done. Has someone found the secret? or is it a scam? Dave From owner-mycology@net.bio.net Mon Sep 12 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!daresbury!trane.uninett.no!eunet.no!nuug!EU.net!uunet!gatekeeper.us.oracle.com!sgiblab!nbn!news From: harris@miwok.nbn.com (Bob Harris) Subject: Re: Fungus tea Message-ID: X-Posted-From: InterNews 1.0.2@ncs-22.nbn.com Lines: 6 Sender: news@nbn.com Organization: North Bay Network's news posting service - not responsible for content References: <34oe2a$ejs@search01.news.aol.com> X-Authenticated: harris on POP host miwok.nbn.com Date: Tue, 13 Sep 1994 04:25:28 GMT I have heard from several people who were using the tea that it seemed to bring on Herpes simplex symptoms more frequently than normal. For what it is worth. Bob Harris harris@bhc.com From owner-mycology@net.bio.net Mon Sep 12 23:00:00 1994 Path: biosci!MOOSE.UVM.EDU!lvaillan From: lvaillan@MOOSE.UVM.EDU (Lisa Vaillancourt) Newsgroups: bionet.mycology Subject: re:teaching thanks Date: 13 Sep 1994 09:43:34 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <9409131643.AA103873@moose.uvm.edu> NNTP-Posting-Host: net.bio.net Hi Bob, I am very intrigued by your "acoustic assay" for Rhizopus pectinases! Do you fill the tube with the filtered culture broth, submerging the carrot disk? How long does it usually take for the weight to drop? How thick do you cut the carrot slices? Thankyou! Lisa Vaillancourt From owner-mycology@net.bio.net Tue Sep 13 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!swrinde!howland.reston.ans.net!swiss.ans.net!newstf01.cr1.aol.com!search01.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: Re: Fungus tea Date: 13 Sep 1994 20:36:05 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 19 Sender: news@search01.news.aol.com Message-ID: <355ghl$310@search01.news.aol.com> References: NNTP-Posting-Host: search01.news.aol.com In article , harris@miwok.nbn.com (Bob Harris) writes: thanks for the comments on Chambuska aka the Manchurian mushroom. The topical application may make sense. I am being inundated with requests....as I write I am talking to G. Lincoff, he too is being flooded with calls. Some responsible physician on Internet should take it on as a cause celebre and get to the bottom of it all. I think there will be appropriate uses, and Herpes may be one application. There was an article published in the Canadian Journal of Botany about the exact identification of the yeasts and bacteria involved. I have misplaced the article but will search for it. I do know that about 10 different names have been used. Anyhow, it getting ridiculous.... stay in touch...more books on the way! paul stamets From owner-mycology@net.bio.net Tue Sep 13 23:00:00 1994 Path: biosci!cibnor.conacyt.mx!dianson From: dianson@cibnor.conacyt.mx ("David Ianson [BE]") Newsgroups: bionet.mycology Subject: Help Date: 14 Sep 1994 11:20:32 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I am trying to find a position in Alaska. I have a PhD in Plant Pathology and have specialized in mycorrhizal fungi and plant-microbe interactions as they are influenced by site disturbances. How can a person from the lower 48 get a job up there. It seems like a very closed system. I would appreciate hearing any advice on the subject. Dave the mycorrhizast From owner-mycology@net.bio.net Tue Sep 13 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!quagga.ru.ac.za!nntp.und.ac.za!pc057.ag.unp.ac.za!TennentG From: TennentG@micr.unp.ac.za (Geoff.Tennant) Newsgroups: bionet.mycology Subject: Re: Growing morel mushrooms Date: Wed, 14 Sep 1994 06:44:20 GMT Organization: University of Natal , Pietermaritzburg, South Africa Lines: 25 Distribution: world Message-ID: References: <94091309470255@hugo.fsufay.edu> NNTP-Posting-Host: pc057.ag.unp.ac.za In article <94091309470255@hugo.fsufay.edu> dhaas@HUGO.FSUFAY.EDU writes: >From: dhaas@HUGO.FSUFAY.EDU >Subject: Growing morel mushrooms >Date: 13 Sep 1994 06:54:43 -0700 >Hello mycos > Does anyone know anything about Gourmet Mushrooms, Inc. They claim that >with a Morel Habitat Kit that you can grow Morels in your back yard. I >thought >this couldn't be done. Has someone found the secret? or is it a scam? >Dave Dave, I seem to remember reading a paper/abstract somewhere that some morels had a symbiotic (mycorrhizal) relationship with various ferns and grasses. If true then surely this IS a scam of note. Only one morel has ever been found in our area (in an area called Ferncliffe!), Geoff Tennant Pietermaritzburg South Africa From owner-mycology@net.bio.net Tue Sep 13 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!swiss.ans.net!newstf01.cr1.aol.com!search01.news.aol.com!not-for-mail From: mycoworld@aol.com (MycoWorld) Newsgroups: bionet.mycology Subject: Re: Growing morel mushrooms Date: 13 Sep 1994 23:00:10 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 22 Sender: news@search01.news.aol.com Message-ID: <355ovq$5mh@search01.news.aol.com> References: <94091309470255@hugo.fsufay.edu> NNTP-Posting-Host: search01.news.aol.com In article <94091309470255@hugo.fsufay.edu>, dhaas@HUGO.FSUFAY.EDU writes: > Is gourmet mushrooms, Inc. Legitimate Yes, they've been around awhile. Read their guarantee carefully - it doesn't say that YOU will be able to grow the morels, only that they will grow some morels off the spawn as a test. It is possible to grow morels outdoors in properly prepared beds, but it is certainly NOT a sure thing. Fungi Perfecti, Northwoods Retail Nursery, and others market similar kits. We tried one and have no results yet - although we got them started in some pretty bad weather conditions (cold and dry). Others report varying degrees of success, but I haven't heard of anyone producing anything like commercial quantities. It doesn't cost a lot to give it a try and you might get lucky. It seems like morels require some specific weather patterns to fruit reasonably. You chances of getting those weather patterns will, of course, be better if you have wild morels fruiting in your vicinity. As far as I know, only Morel Mountain (now owned by Terry Farms), ever produced commercial quantities indoors. -Jerry - The Mushroom Growers' Newsletter MycoWorld@aol.com From owner-mycology@net.bio.net Tue Sep 13 23:00:00 1994 Path: biosci!rutgers!gatech!newsxfer.itd.umich.edu!europa.eng.gtefsd.com!howland.reston.ans.net!swrinde!cs.utexas.edu!uunet!news.sprintlink.net!news.world.net!news.teleport.com!usenet From: "Ralph D. Arnold" Newsgroups: bionet.mycology Subject: FAQ info: email address Date: Tue, 13 Sep 94 18:10:56 CST Organization: Teleport - Portland's Public Access (503) 220-1016 Lines: 19 Message-ID: <76256.rarnold@teleport.com> Reply-To: NNTP-Posting-Host: ip-c01.teleport.com X-Minuet-Version: Minuet1.0_Beta_16 X-POPMail-Charset: English I'd like to set up an amateur FAQ file for people seeking information about mycology (hunting/eating/growing). If you are a representative of a company or club or other relevent organization, please email me your details: Name of organization and contact person(s) email address fax and phone numbers mailing address description of your group/company I'll try to organize this info and make it available. Thanks! Ralph D. Arnold P.O. Box 889 Oregon City, OR 97045 rarnold@teleport.com From owner-mycology@net.bio.net Tue Sep 13 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!europa.eng.gtefsd.com!newsxfer.itd.umich.edu!nntp.cs.ubc.ca!news.UVic.CA!spruce.pfc.forestry.ca!PFC.Forestry.CA!RWINDER From: rwinder@PFC.Forestry.CA (Richard Winder) Subject: Re: Cultivation of Cantharellus cibarius, mykhorrhiza Message-ID: <1994Sep12.192600.28156@spruce.pfc.forestry.ca> Sender: news@spruce.pfc.forestry.ca Nntp-Posting-Host: pfc.pfc.forestry.ca Reply-To: rwinder@PFC.Forestry.CA Organization: Forestry Canada (Pacific Forestry Centre) References: <1994Sep6.221240.28767@spruce.pfc.forestry.ca>,<34odn1$efb@search01.news.aol.com> Date: Mon, 12 Sep 1994 19:26:00 GMT Lines: 33 In article <34odn1$efb@search01.news.aol.com>, stamets1@aol.com (Stamets 1) writes: >In article <1994Sep6.221240.28767@spruce.pfc.forestry.ca>, > >thanks for the info. I think Nils Fries is the one who published the spore >germination w/R. glutinus article. check out his bibliography in the >article you sighted. I should think it would be there. I back-checked through all of the previous work Nils cited on this topic. Unfortunately, I could find no reference to C. cibarius. The paper is probably later, or disconnected from the chain of references that I have in my posession. There is a paper in which Nils tries the R. glutinus/charcoal technique on Laccaria laccata, Amanita muscaria, Lactarius helvus, Paxillus involutus, and Leccinum scabrum. (Fries, N., 1978. Basidiosproe germination in some mycorrhiza-forming hymenomycetes. Trans. Brit. Mycol. Soc. 70:319-324). It looks like a good general technique for germinating most any basidiospore. There are two references which I couldn't check on because I don't have ready access to them: Fries, N. 1941. Uber die Sporenkeimung bei einigen Gasteromyceten und Mykorrhiza-bildenden Hymenomyceten. Archiv für Mikrobiologie 12:266-284. Fries, N. 1943. Untersuchungen über Sporenkeimung und Mycelentwicklung botenbewohndender Hymenomyceten. Symbolae Botanicae Upsalinense 6:1-81. Sorry I couldn't find anything that would be of more specific help. -RSW RICHARD WINDER Title: Research Scientist Canadian Forest Service Phone: (604) 363-0773 Victoria, B.C. Internet: RWINDER@A1.PFC.Forestry.CA From owner-mycology@net.bio.net Tue Sep 13 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!uunet!newstf01.cr1.aol.com!search01.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: Re: Growing morel mushrooms Date: 14 Sep 1994 14:44:07 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 10 Sender: news@search01.news.aol.com Message-ID: <357g9n$i88@search01.news.aol.com> References: NNTP-Posting-Host: search01.news.aol.com In article , TennentG@micr.unp.ac.za (Geoff.Tennant) writes: i have been growing morels for several years outdoors. our success is about 75%, and is weather-dependent. we have published quite a bit on the subject as you probably know. paul stamets From owner-mycology@net.bio.net Wed Sep 14 23:00:00 1994 Path: biosci!BADLANDS.NODAK.EDU!jweiland From: jweiland@BADLANDS.NODAK.EDU (John J Weiland) Newsgroups: bionet.mycology Subject: Fungal Promoters Date: 15 Sep 1994 09:53:08 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Has anyone seen a publication comparing RNA pol II promoters from filamentous fungi? Also, can anyone direct me to sources for: a) the hygromycin B resistance gene b) a commercial BAC vector c) the cheapest source of V-bottom 96-well microtitre plates for use in MJR Thermocycler. Thanks in advance! John Weiland jweiland@badlands.nodak.edu From owner-mycology@net.bio.net Thu Sep 15 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!pipex!uknet!daresbury!not-for-mail From: "Peter J. Punt,MBL-TNO,Rijswijk,The Neth." Newsgroups: bionet.mycology Subject: abstracts Date: 16 Sep 1994 09:37:21 +0100 Organization: TNO Nutrition and Food Research Lines: 181 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <35blg1$and@mserv1.dl.ac.uk> Original-To: mycology@dl.ac.uk STRAIN IMPROVEMENT STRATEGIES FOR THE OVERPRODUCTION OF FUNGAL PROTEINS BY FILAMENTOUS FUNGI Jan C.Verdoes, Peter J. Punt and Cees A.M.M.J. van den Hondel. Applied Microbiology and Biotechnology (1994) in press I. INTRODUCTION Filamentous fungi may be isolated from a large number of different natural environments (e.g. from soil, organic waste and as pathogens from plants and animals). These different habitats and the competition with other micro-organisms in the same place may explain the extraordinary metabolic diversity of many filamentous fungi, as illustrated by the wide variety of carbon and nitrogen sources they can use for growth. The availability of a multitude of biosynthetic and catabolic pathways necessary for this metabolic diversity makes filamentous fungi potential production organisms for a wide range of low molecular-weight primary and secondary metabolites, and even more importantly, intra- and extracellular hydrolytic enzymes. In effect, filamentous fungi or the enzymes they produce have been used in food and foodprocessing industry for many decades which resulted in a so-called GRAS status (Generally Recognized As Safe; U.S Food and Drug Administration) for some of the species (such as Aspergillus niger and A. oryzae).=20 The industrial potential of filamentous fungi has stimulated research in the development of large scale fermentation processes, down-stream processing and methods for strain improvement (see below section II), the results of which further contributed to the use of filamentous fungi in today's industry. The development of molecular genetic techniques (in particular gene transfer technology) for most "industrial" species (for reviews see Finkelstein, 1991; May, 1992) has even further opened the way for industrial exploitation of these organisms in particular for the production of enzymes encoded by fungal, and even non-fungal, genes. In this chapter we give an overview of research carried out towards improved production of extracellular fungal proteins. In general, the proteins of interest are hydrolytic enzymes involved in the degradation of polysaccharides, which degradation product is used by the fungi as carbon source.=20 =0CTHE EFFECT OF MULTIPLE COPIES OF THE UPSTREAM REGION ON EXPRESSION OF THE ASPERGILLUS NIGER GLUCOAMYLASE-ENCODING GENE=20 Jan C. Verdoesa&, Peter J. Punta, Adriaan H. Stouthamerb and Cees A.M.J.J. van den Hondela Gene (1994) in press SUMMARY The regulation of transcription of the glucoamylase- encoding gene (glaA) of Aspergillus niger was studied. To facilitate this study a reporter strain containing a fusion of the glaA promoter (PglaA) of A. niger to the =FE-glucuronidase- encoding gene (uidA) of Escherichia coli was constructed. To analyze whether regulatory proteins are involved in the regulation of glaA, multiple copies of PglaA were introduced into this reporter strain. Analysis of the resulting strains revealed that introduction of an increasing number of PglaA copies resulted in lower expression of the uidA reporter gene and the endogenous glaA gene in cultures cultivated on different inducing carbon sources. However, repression by xylose was not influenced by the copy number of PglaA. These results indicate that the expression of genes under control of PglaA are regulated by specific trans-acting regulatory protein(s). Deletion analysis of PglaA indicated that regulatory proteins interact with DNA sequences within 0.5-kb upstream from the ATG, whereas sequences between about 0.8 and 0.5-kb upstream from the ATG are required for high-level expression of glaA. =0CCHARACTERIZATION OF AN EFFICIENT GENE CLONING STRATEGY FOR ASPERGILLUS NIGER BASED ON AN AUTONOMOUSLY REPLICATING PLASMID: CLONING OF THE NICB GENE OF A. NIGER Jan C. Verdoesa*, Peter J. Punta, Pieter van der Bergab, Fons Debetsb, Adriaan H. Stouthamerc and Cees A.M.J.J. van den Hondela=20 Gene (1994) in press SUMMARY The development of an improved gene cloning strategy by complementation of mutant alleles in Aspergillus niger is described. The strategy is based on the use of a fungal autonomously replicating vector, pAB4-ARp1. This vector was constructed by the introduction of a previously described sequence (AMA1), involved in autonomous replication, into a pyrG integrative vector, pAB4-1. With vector pAB4-ARp1, a 10 to 100-fold increase in transformation frequency was obtained, as compared to pAB4-1. Furthermore, the transformation frequency of a cotransformed plasmid is also increased using pAB4-ARp1. A. niger transformants containing pAB4-ARp1 are mitotically unstable. Cotransformed plasmids strictly cosegregated with the autonomously replicating vector, as a result of recombination between both vectors. The use of pAB4- ARp1 in gene cloning was demonstrated by the complementation of two linkage group VII-specific A. niger mutants. Complementation of a lysF mutant was achieved by cotransformation of pAB4-ARp1 with total genomic A. niger DNA ("instant bank"). A nicB-deficient A. niger was complemented by cotransformation with pAB4-ARp1 and an A. niger cosmid library. The complementing DNA was re-isolated from a Nic+ transformant by transforming E. coli with total genomic DNA of this transformant. Gene disruption and genetic analysis was carried out to prove that the previous unknown A. niger nicB gene has been cloned.=20 =0CTHE COMPLETE KARYOTYPE OF ASPERGILLUS NIGER: THE USE OF INTRODUCED ELECTROPHORETIC MOBILITY VARIATION OF CHROMOSOMES FOR GENE ASSIGNMENT STUDIES.=20 Jan C. Verdoes*, M. Regina Calila, Peter J. Punt, Fons Debetsa, Klaas Swarta, Adriaan H. Stouthamerb and Cees A.M.J.J. van den Hondel. Molecular General Genetics (1994) 244:75-80 Summary A method is described for unambiguous assignment of cloned genes to Aspergillus niger chromosomes by CHEF-Southern analysis. All of the 8 linkage groups (LGs) with the exception of LG VII have previously been assigned to specific chromosomal bands in the electrophoretic karyotype of A. niger (Debets et al. (1990) MGG 224: 264-268). Using a LG VII specific probe (nicB gene of A. niger) we have shown that LG VII corresponds to a chromosome of about 4.1 Mb. Furthermore, genetic localization of three unassigned genes (glaA, aglA and pepA) in strains in which these genes were replaced using a selectable marker gene led to a revised karyotype with respect for the chromosomes corresponding LG VIII and VI. The revised electrophoretic karyotype reveals only 5 distinct bands. The presence of 3 pairs of equally sized chromosomes precluded assignment of genes to one specific chromosome in the wild- type strain. However, unambiguous chromosome assignment of cloned genes using CHEF-Southern analysis was demonstrated using a set of A. niger strains with introduced chromosomal size variation. The availability of these tester strains obviates the need of isolating or constructing mutant strains for the purpose of chromosome assignment. =0CEVALUATION OF MOLECULAR = AND GENETIC APPROACHES TO GENERATE GLUCOAMYLASE OVERPRODUCING STRAINS OF ASPERGILLUS NIGER=20 =20 Jan C. Verdoes1 *, Anne D. van Diepeningen2, Peter J. Punt1, Alfons J.M. Debets2, Adriaan H. Stouthamer3 and Cees A.M.J.J. van den Hondel1 Journal of Biotechnology 36 (1994) 165-175 Summary To evaluate the possibility to improve glucoamylase (GLA) production in A. niger strains, carrying multiple copies of the GLA encoding gene (glaA), additional glaA copies were introduced either by genetic recombination or retransformation. For strains to be used in such experiments a genetic analysis was first carried out. The results of this analysis clearly revealed that in each transformant integration had occurred at a chromosome corresponding to a single linkage group. The GLA production per gene copy showed considerable variation in these strains, indicating a clear effect of the site of integration on gene expression. Introduction of additional gene copies by genetic recombination experiments was carried out for different combinations of strains, carrying glaA copies in different chromosomes. The introduction of additional glaA gene copies by genetic recombination did not result in a considerable increase in GLA production compared to the parental strains. In some strains recombination resulted in genetic instability, observed by the frequent loss of glaA copies. Also retransformation of multi-copy glaA strains did not result in increase in GLA production. In several strains even a decrease in GLA production was found after retransformation. Southern analysis of these transformants suggested that newly introduced gene copies were heavily rearranged which partly explains why GLA production was not increased. Further analysis of one such transformant provided evidence that the overexpression of the glaA gene is limited by the amount of trans-acting regulatory protein(s) available. From owner-mycology@net.bio.net Thu Sep 15 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!agate!library.ucla.edu!news.mic.ucla.edu!unixg.ubc.ca!nntp.cs.ubc.ca!news.UVic.CA!spruce.pfc.forestry.ca!PFC.Forestry.CA!RWINDER From: rwinder@PFC.Forestry.CA (Richard Winder) Subject: Re: Growing morel mushrooms Message-ID: <1994Sep15.202940.12093@spruce.pfc.forestry.ca> Sender: news@spruce.pfc.forestry.ca Nntp-Posting-Host: pfc.pfc.forestry.ca Reply-To: rwinder@PFC.Forestry.CA Organization: Forestry Canada (Pacific Forestry Centre) References: <94091309470255@hugo.fsufay.edu> Date: Thu, 15 Sep 1994 20:29:40 GMT Lines: 48 In article <94091309470255@hugo.fsufay.edu>, dhaas@HUGO.FSUFAY.EDU writes: >Hello mycos > > Does anyone know anything about Gourmet Mushrooms, Inc. They claim that >with a Morel Habitat Kit that you can grow Morels in your back yard. I thought >this couldn't be done. Has someone found the secret? or is it a scam? > >Dave > Encouraging morels to grow in your back yard has always been possible, and is often successful- it is not exactly the same thing as cultivation though, since success depends on the habitat, weather, phase of the moon, etc. All you are doing is introducing inoculum into an area, adjusting pH, nutrients, etc., and hoping that it will fruit one day (assuming morel inoculum isn't already in the area). What *is* difficult is farming morels dependably, on a schedule, under controlled conditions. A person named Ower, along with others, won two U.S. patents for generating morels under controlled conditions- a Michigan company is supposed to be scaling up production of morels based on these patents. Unfortunately, the methods seem to be difficult (if not impossible) to duplicate in most labs I've heard about. The methods involve careful control of temperature, humidity, and free water in the substrate. Other workers have more or less defined the conditions under which sclerotial primordia can be formed in agar, partly basing their ideas on the patent work. If a split plate is used, with water agar on one side and nutrient agar on the other, mycelium inoculated on the plain agar side will import nutrients from the nutrient rich section to form sclerotia. I've duplicated this effect in my lab with M. deliciosa, and by using malt extract/jerusalem artichoke agar as a nutrient source, with some ground seashell thrown in the plain agar side, I have not only generated tons of sclerotia, but I've noticed the generation of substantial amounts of tissue on a few (5%) of the petri dish covers where free water accumulates as condensation. It is not enough to form an ascocarp, but the cells are clearly forming a parenchymatous tissue mass about an inch wide and several mm thick in some cases (much larger than a sclerotial primordium). This tissue generation doesn't appear to come about in the same way that Ower illustrates in some of his work- perhaps there is more than one avenue for generating tissue in this genus. The tissue eventually turns darker brown and ceases growth, probably due to limited water and production of staling or inhibitory substances. With a better water wick and a healthy dose of ascorbic acid (pH balanced) to take care of staling compounds, I'm sure it wouldn't be too difficult to generate at least a partial ascocarp. Hopefully, a simple method like this could dilute patent infrigement problems somewhat (which would please me to no end). -RSW RICHARD WINDER Title: Research Scientist Canadian Forest Service Phone: (604) 363-0773 Victoria, B.C. Internet: RWINDER@A1.PFC.Forestry.CA From owner-mycology@net.bio.net Thu Sep 15 23:00:00 1994 Path: biosci!bcm!news.msfc.nasa.gov!europa.eng.gtefsd.com!howland.reston.ans.net!gatech!nntp.msstate.edu!saimiri.primate.wisc.edu!news.doit.wisc.edu!news From: MULROY@EPISAS.EPI.WISC.EDU (Bob Mulroy) Newsgroups: bionet.mycology Subject: Kombucha-cha-cha Date: 16 Sep 1994 19:09:09 GMT Organization: Martian Home Extention Service Lines: 19 Distribution: world Message-ID: <35cqgl$icj@news.doit.wisc.edu> NNTP-Posting-Host: episas.epi.wisc.edu X-News-Reader: VMS NEWS 1.24 I hate to add another wit of info to the kombucha mystery, so I'll add two: 1. Kombucha means "Giant Kelp Tea" "Kombu", the dried leaves of the giant pacific kelp is an almost inescapable foodstuff in Japan. I can see where a fermented beverage of kombu would be at least wholesome -if not an elixer of immortality. 2. In Chicago I went to Yaohan, a complete mall for asians. In the tea section I found a hexagonal tin labled Kombucha. It couldn't have weighed ten grams. All the other writing was in japanese and my curiousity was smaller than the fifteen-dollar price tag. I'm getting oplder and uglier everyday, so I hope somebody figures this out soon! Bob Mulroy, Successful and ruthless monoparent, Unemployed Chief Techie Weiner 9-A University Houses, Madison WI 53705. (608) 233-4672 Bob in '96!!! --------------------------R. U. Hiring?------------------------------------- From owner-mycology@net.bio.net Thu Sep 15 23:00:00 1994 Path: biosci!BULL.CC.USM.EDU!GLEN_SHEARER From: GLEN_SHEARER@BULL.CC.USM.EDU (GLEN SHEARER) Newsgroups: bionet.mycology Subject: Ireland, et. al. DNA extraction Date: 16 Sep 1994 12:25:01 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: <940916.14241588.053475@USM.CP6> NNTP-Posting-Host: net.bio.net I heard someone mention at a recent talk a good method to extract DNA from filamentous fungi. Unfortunately the speaker could only recall that one of the authors was Ireland and that it was probably in Fungal Genetics Newsletter. Anyone know if this sounds like a recent FGN article? If so, how well does it work in your hands? Many thanks Glen From owner-mycology@net.bio.net Fri Sep 16 23:00:00 1994 Path: biosci!daresbury!trane.uninett.no!sunic!uunet!newstf01.cr1.aol.com!newsbf01.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: Re: Kombucha-cha-cha Date: 16 Sep 1994 19:39:06 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 10 Sender: news@newsbf01.news.aol.com Message-ID: <35daaq$2o1@newsbf01.news.aol.com> References: <35cqgl$icj@news.doit.wisc.edu> NNTP-Posting-Host: newsbf01.news.aol.com In article <35cqgl$icj@news.doit.wisc.edu>, MULROY@EPISAS.EPI.WISC.EDU (Bob Mulroy) writes: I have had two interviews in the past 24 hours with the L.A. times and New Age journal about kambuska. it is achieving a fever-pitch out there. I have not heard of the kelp connection... that is news to me. paul stamets From owner-mycology@net.bio.net Fri Sep 16 23:00:00 1994 Path: biosci!agate!library.ucla.edu!csulb.edu!nic-nac.CSU.net!charnel.ecst.csuchico.edu!yeshua.marcam.com!zip.eecs.umich.edu!panix!ddsw1!godot.cc.duq.edu!news.duke.edu!news-feed-1.peachnet.edu!gatech!swiss.ans.net!newstf01.cr1.aol.com!newsbf01.news.aol.com!not-for-mail From: burrite@aol.com (Burrite) Newsgroups: bionet.mycology Subject: Re: Mushroom-hunter's group? or no? Date: 17 Sep 1994 14:32:08 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 13 Sender: news@newsbf01.news.aol.com Message-ID: <35fcn8$ikp@newsbf01.news.aol.com> References: <3451nm$64p@search01.news.aol.com> NNTP-Posting-Host: newsbf01.news.aol.com wtcat@aol.com (WTCat) writes: >>Contact Brian McNett (BrainMc@aol.com). He puts together MycoInfo, an online mushroom newsletter. Lots of fun for those of us at hunt and eat the fleshy fungus. Could you send me some info on the newsletter? I'd sure like to start growing mushrooms but don't have a clue as to where to start. Thanks in advance burrite@aol.com From owner-mycology@net.bio.net Fri Sep 16 23:00:00 1994 Path: biosci!agate!library.ucla.edu!news.ucdavis.edu!rocky!ez040548 From: ez040548@rocky.ucdavis.edu (Faust) Newsgroups: bionet.mycology Subject: Toxin absorbed from Substrate? Date: 17 Sep 1994 18:07:29 GMT Organization: Information Resources, UC Davis Lines: 19 Message-ID: <35fb91$hep@mark.ucdavis.edu> NNTP-Posting-Host: rocky.ucdavis.edu X-Newsreader: TIN [version 1.2 PL2] I have a question requarding the metabolic processes and absortion of potential toxins from the substrate into the fruit-body of mushrooms. I recently began composting and I am planning on growing any mushroom species which will take in my yard, I had in mind Stropharia rugoso-annulata (King Stropharia) and other edible mushrooms perhaps woodears and oyster mushrooms, the question I have is whether the compost of Oleander (a toxic bush/tree) will contain the toxins that the original plant did, and if it does, will these toxins be absorbed by the fungi. Obviously the toxins in this case are organic, but I also have another question, I have access to "sludge" from a waste treatment plant, and it contains larger quantitys of heavy metals than does normal soil, I am wondering if -those- would be absorbed. Also both of these questions would apply to normal vegetables as well if anyone knows anything about that, and if there is any appreciable difference in absortion. My e-mail address in case it is not posted it whporter@ucdavis.edu. Thanks, -Bill From owner-mycology@net.bio.net Fri Sep 16 23:00:00 1994 Path: biosci!daresbury!trane.uninett.no!sunic!uunet!spool.mu.edu!howland.reston.ans.net!swiss.ans.net!newstf01.cr1.aol.com!newsbf01.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: Need information on Poria cocos/Cordyceps sinense Date: 16 Sep 1994 19:46:02 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 27 Sender: news@newsbf01.news.aol.com Message-ID: <35danq$2rf@newsbf01.news.aol.com> NNTP-Posting-Host: newsbf01.news.aol.com Subj: Poria cocos/Cordyceps sinense Date: 94-09-16 12:27:48 EDT From: Stamets 1 To: BioNet I am searching for cultivation parameters for Poria cocos, Cordyceps sinense and Fomitopsis pinicola. Any help would be much appreciated. Most references have been in Chinese and the abstracts have not been very enlightening. Additionally, I would like to bring to your attention to theavailability of the American Type Culture Collection's catalogue is now available on diskette. I loaded the entire library (6 diskettes) onto my Macintosh (PC also available). More than 30,000 cultures can be searched. The program has been intelligently designed with many good features. Very cool. The cost of printing their catalogue has become prohibitive so it has not been updated (i.e. re-printed) since 1991. paul stamets From owner-mycology@net.bio.net Fri Sep 16 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!agate!dog.ee.lbl.gov!news.cs.utah.edu!emba-news.uvm.edu!moose.uvm.edu!wschaeff From: "Warren I. Schaeffer" Subject: New Journal Message-ID: <1994Sep17.190852.17256@emba.uvm.edu> Originator: wschaeff@moose.uvm.edu Sender: news@emba.uvm.edu Organization: EMBA Computer Facility, University of Vermont Date: Sat, 17 Sep 1994 19:08:52 GMT Lines: 115 Greetings! I hesitated to use the newsgroup to disseminate the following information but, on reflection, knew that I would want to know about a new journal with direct relevance to my research. However, if this posting offends anyone, I would appreciate their responding directly to me rather than by cluttering the newsgroup. Therefore, I would like to call your attention to a new journal "METHODS IN CELL SCIENCE"which is to be published by Kluwer Academic Publishers of Dordrecht, The Netherlands. I have recently accepted the position of Editor-in-Chief. Below are the Aims & Scope of the journal for your perusal. In addition, I am including the initial Area Descriptors which will be the areas under which prospective authors will submit their manuscripts. I am currently in the process of assembling the Editorial Board and hope to have it appointed within the next two months. ************************ AREA DESCRIPTORS: Biotechnology; Cellular and Molecular Toxicology; Cell Biology; Developmental Biology; Growth, Differentiation or Senescence; Genetics; Immunology; Infectious Diseases/Cellular Pathology; Neurobiology; Plant Biology and Virology. ************************ AIMS AND SCOPE: The aim of METHODS IN CELL SCIENCE is to provide _the_ forum for publishing the most up-to-date, cutting edge, methods developed during the course of cell science research involving vertebrates, inverte- brates, plants or single-celled prokaryotic or eukaryotic organisms. The research relevant to this publication is ordinarily conducted using in vitro systems rather than the whole animal or plant, an exception being research in which the animal or plant itself must be viewed microscopically. Manuscripts will also be accepted which describe the design or construction of equipment, media, or quality control procedures that are ancillary to cellular research. Descriptions of the subheadings used in the Table of Contents of METHODS IN CELL SCIENCE (found in the "Instructions for Authors") provide prospective authors with additional information about the subsections of the journal that would be most appropriate for their manuscripts. Manuscripts will be peer reviewed and will only be published in METHODS IN CELL SCIENCE if they provide sufficient detail to enable a researcher to successfully duplicate the method using only the protocol described in the manuscript. In contrast, methods published in most scientific journals, whose emphasis is different than that of METHODS IN CELL SCIENCE, often lack the details required for readers to do so without prior experience with the method or by consulting the author(s). All manuscripts submitted will describe methods original to the author's laboratory which either have never before been published or which were originally published as part of a research paper submitted to another journal. To be acceptable for publication in METHODS IN CELL SCIENCE, rewritten methods must now contain the original citation and all of the detail which could not be included in the original manuscript. A rewritten method, published in METHODS IN CELL SCIENCE, will provide an opportunity for the author to reach a wider audience. SPECIAL ISSUES will devote an entire issue to methodology involved in a specialized area of cellular research. Such specialized areas might, for example, include: Methods in Invertebrate Cell Clulture Research; Methods in Oral Streptococci Research; Research Techniques Involving Caenorhabditis elegans; Methods in Plant Molecular Biology; Molecular Genetic Techniques Using Zebra Danio or Drosophila or, etc. Editors of special issues will solicit, compile and review the necessary manuscripts to complete such an issue. Editors of special issues will often be invited to participate but authors interested in compiling such an issue should contact the Editor-in-Chief. REVIEW ARTICLES: Both mini reviews and full-length reviews, covering various aspects of developments in methodological approaches, equipment, media or quality control procedures related to cellular research will be accepted. Usually they will be invited, but authors interested in contributing such a paper should contact the Editor-in-Chief, describing the proposed review, before submitting a manuscript. A mini review will ordinarily span no more than four to five printed pages whereas full reviews may be ten or more printed pages. *********************** I do hope that you are sufficiently interested in the raison d'etre of the journal that you will join me in this enterprise by submitting manuscripts to it. I believe that this will not be just another journal (we probably have too many now) but will serve a definite purpose. The methods presented will be as complete as it is possible to write them. One should, by reading the paper, be "transported" to the lab bench in the author's laboratory and be following along as the protocol is demonstrated. I firmly believe that there is a place for this kind of presentation. One plan for the journal includes publishing in a "three-hole punched" format so that either the entire issue, or one or more of the methods can be placed right into the laboratory's methods notebook. This may be a possibility. Initially, the journal will appear quarterly, but I hope that with rising interest, it can ultimately appear bimonthly or even monthly. Please contact me at the address below if you wish to obtain an initial copy of the "Information for Authors" or if you are interested in a subscription to the journal. You may also direct your institution to do the same if they are interested in a subscription for the library. If your library currently subscribes to the "Journal of Tissue Culture Methods", they will automatically be contacted by the publishers as that journal will be replaced by METHODS IN CELL SCIENCE. Many of you have wanted to see a journal such as this one available and now I believe this has come to fruition. With the usual few delays in getting something new underway, METHODS IN CELL SCIENCE should be available at the beginning of the new year. I would appreciate receiving any comments you may have regarding this new enterprise (however, please respond directly to me rather than through the newsgroup) and, especially, your manuscripts and suggestions for Special Issues. ___________________________________________________________________________ Warren I. Schaeffer, University of Vermont, Markey Center for Molec. Genet., Microbiol. & Molec. Genetics Dep't., 117 Stafford Bldg. Burlington, Vt.05405 Phone: (802)656-2290 * Fax:(802)656-8749 * e-mail: wschaeff@moose.uvm.edu ____________________________________________________________________________ -- ___________________________________________________________________________ Warren I. Schaeffer "Never, for the sake of peace and quiet Microbiology & Molec. Genetics deny your own experience or convic- 117 Stafford Building tions" Dag Hammarskjold From owner-mycology@net.bio.net Fri Sep 16 23:00:00 1994 Path: biosci!agate!library.ucla.edu!csulb.edu!nic-nac.CSU.net!charnel.ecst.csuchico.edu!yeshua.marcam.com!zip.eecs.umich.edu!panix!ddsw1!godot.cc.duq.edu!news.duke.edu!news-feed-1.peachnet.edu!gatech!swiss.ans.net!newstf01.cr1.aol.com!newsbf01.news.aol.com!not-for-mail From: burrite@aol.com (Burrite) Newsgroups: bionet.mycology Subject: Re: Mushroom-hunter's group? or no? Date: 17 Sep 1994 14:33:03 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 5 Sender: news@newsbf01.news.aol.com Message-ID: <35fcov$il6@newsbf01.news.aol.com> References: <3451nm$64p@search01.news.aol.com> NNTP-Posting-Host: newsbf01.news.aol.com Oh, sorry. I obviously really don't have a clue :-) Meant to send that last message via e-mail. Apologies. From owner-mycology@net.bio.net Sun Sep 18 23:00:00 1994 Path: biosci!agate!msuinfo!harbinger.cc.monash.edu.au!news.uwa.edu.au!newsman.csu.murdoch.edu.au!esmac4.csu.murdoch.edu.au!user From: walker@essun1.murdoch.edu.au (Colin Walker) Newsgroups: bionet.mycology Subject: Food Value of Cultivated Mushrooms Followup-To: bionet.mycology Date: 19 Sep 1994 07:32:34 GMT Organization: Murdoch Lines: 31 Distribution: world Message-ID: NNTP-Posting-Host: esmac4.csu.murdoch.edu.au I am searching for food value measurements ( you know the stuff, vitamins, trace elements, other minerals) on the cultivated mushrooms, particularly Agaricus spp., but I'll take anything. Those of you who know the literature might be aware that there is a bit out there in texts and journal articles. I have been accessing some of that, especially through CAB, but I'm sure there is a body of additional information that has been collected by many and just accumulates on files. This is a shame because while data on many other plant food species is accumulating and agronomically-minded people are now working on fertiliser effects on yields and tissue concentrations, optimising the ratios of nutrients using principles like DRIS, a