From owner-mycology@net.bio.net Sat Oct 01 23:00:00 1994 Path: biosci!agate!spool.mu.edu!howland.reston.ans.net!swiss.ans.net!newstf01.cr1.aol.com!newsbf01.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: identify of microorganisms composing Kambucha Date: 2 Oct 1994 11:47:10 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 9 Sender: news@newsbf01.news.aol.com Message-ID: <36mklu$bc1@newsbf01.news.aol.com> NNTP-Posting-Host: newsbf01.news.aol.com For those interested in reading about the identity of the organisms composing Kambucha see the Mycologist, Vol 7 (1): 12-13. 1993. paul stamets From owner-mycology@net.bio.net Sun Oct 02 23:00:00 1994 Path: biosci!CS.Arizona.EDU!uunet!cs.utexas.edu!not-for-mail From: maxwell@calshp.cals.wisc.edu Newsgroups: bionet.mycology Subject: Growing morel mushrooms Date: 2 Oct 1994 21:07:39 -0500 Organization: UTexas Mail-to-News Gateway Lines: 41 Sender: nobody@cs.utexas.edu Message-ID: <9410030209.AA27447@calshp.cals.wisc.edu> References: <9410030140.AA26929@calshp.cals.wisc.edu> NNTP-Posting-Host: news.cs.utexas.edu > > > >> I seem to remember reading a paper/abstract somewhere that some morels had > >>a symbiotic (mycorrhizal) relationship with various ferns and grasses. If > >>true then surely this IS a scam of note. > > I think I've seen reports for at least one type of tree, too. I'm pretty sure that Morchella esculenta forms ectomycorrhizae on American Elm, though I can't find where I read this (always misplacing references). Alexopolous says about morels "many if not all are mycorrhial but this has not been proved." > > >I don't think this is the case; if morels do engage in mycorrhizae, then > >its certainly not of the obligate kind. The fact that Morels do so well > >in the wake of disturbance, especially forest fires, seems to rule aginst > >their being obligate mycorrhizal fungi. Certainly, they are not obligate mycorrhizae, since it is possible to isolate vegetative cultures of the fungus simply by plating a piece of the ascocarp on media. > > Under these conditions they operate more like cryophiles- microbes > which thrive best in very cold, nutrient rich soils, but are usually > outcompeted in warmer soils. Whole forests used to be burnt down in Russia > to encourage morels- a practice which surely would not have become popular > if it was necessary to have mycorrhizae. I have to disagree. A stable mycorrhizal reationship should encourage vegetative growth of the fungus. Sex generally occurs when a nutrient source becomes limited. Burning forests would cause the nutrient source of the fungi (the living tree) to be absent. At this point, the organism puts all of its resources into sex, and long distance dispersal. -enjoy. A -- David Maxwell 382 Russell Labs maxwell@calshp.cals.wisc.edu 1630 Linden Drive dlm@plantpath.wisc.edu From owner-mycology@net.bio.net Sun Oct 02 23:00:00 1994 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!swrinde!pipex!lyra.csx.cam.ac.uk!warwick!news.shef.ac.uk!sunc!mb1gt From: mb1gt@sunc.sheffield.ac.uk (G Turner) Newsgroups: bionet.mycology Subject: Neurospora expression vectors? Date: 3 Oct 1994 09:19:51 GMT Organization: Academic Computing Services, Sheffield University Lines: 6 Message-ID: <36oibn$rjb@hippo.shef.ac.uk> NNTP-Posting-Host: sunc.shef.ac.uk X-Newsreader: TIN [version 1.2 PL2] Does anyone know of the existence of useful expression vectors for overexpression of proteins in N. crassa? Geoff Turner Sheffield G.Turner@sheffield.ac.uk From owner-mycology@net.bio.net Tue Oct 04 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!EU.net!sunic!trane.uninett.no!astfgl.edb.tih.no!pc013.kjemi.tih.no!bjorn From: bjorn@kjemi.tih.no (Bjorn Ness) Newsgroups: bionet.general,bionet.mycology,bionet.protista Subject: Xylitol - production from xylan (hemicellulose) - fermentation Date: Wed, 5 Oct 1994 11:18:58 Organization: Kjemiavdelingen Lines: 22 Message-ID: NNTP-Posting-Host: pc013.kjemi.tih.no Keywords: xylitol- production, xylose, xylan, hemicellulose, enzymes, fermentation X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Xref: biosci bionet.general:11412 bionet.mycology:975 bionet.protista:180 Information needed - please help ! A student group at our college are doing a projectwork on the isue: production of xylitol from xylans (wood-hemicellulose). Can anyone give us information and/or referenses regarding microorganisms producing enzymes for degradation of hemicellulose to xylose and microorganisms producing enzymes witch reduses xylose to xylitol. Please answeare by E-mail. Thank's Bjorn Arne Nass Sør-Trøndelag College bjorn@kjemi.tih.no From owner-mycology@net.bio.net Tue Oct 04 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!europa.eng.gtefsd.com!library.ucla.edu!news.mic.ucla.edu!news.bc.net!vanbc.wimsey.com!pme20.pomo.wis.net!user From: margots@wimsey.com (Margot Sinclair) Newsgroups: bionet.mycology Subject: help! need info-odourless compost Date: 5 Oct 1994 01:24:25 GMT Organization: Marclaire Communications Lines: 15 Message-ID: NNTP-Posting-Host: pme20.pomo.wis.net I really would appreciate it if anyone can provide some information about odourless compost for mushroom farms. In some places, odour is considered an environmental hazard. So I'm trying to find the latest technology available that's environmentally-friendly (ie. odourless). I heard that Pennsylvania and the Netherlands are two sites with new technology. This is not an area in which I specialize . . . so in advance, I thank you for your patience and your assistance. Please respond by e-mail to margots@wimsey.com Thanks! -- margots@wimsey.com From owner-mycology@net.bio.net Tue Oct 04 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!swiss.ans.net!newstf01.cr1.aol.com!newsbf01.news.aol.com!not-for-mail From: stametsfam@aol.com (Stametsfam) Newsgroups: bionet.mycology Subject: Re: help! need info-odourless compost Date: 5 Oct 1994 09:33:03 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 13 Sender: news@newsbf01.news.aol.com Message-ID: <36u9uf$ajc@newsbf01.news.aol.com> References: NNTP-Posting-Host: newsbf01.news.aol.com In article , margots@wimsey.com (Margot Sinclair) writes: Ostrom's Mushroom Farms, here in Olympia, has reduced its odiferousness significantly over the past few years. They collect leachate and re-spray it over the compost continuously during Phase I. This oxygenation of the leachate seems to have a significant impact on ammonifying bacteria. You might want to contact Bill Street Jr. or Sr. at 206-491-1410 to discuss their process. Good luck. Paul Stamets From owner-mycology@net.bio.net Tue Oct 04 23:00:00 1994 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!swrinde!howland.reston.ans.net!torn!news.unb.ca!upei.ca!peinet.pe.ca!peinet.pe.ca!not-for-mail From: bncraig@bud.peinet.pe.ca (Brian N. Craig) Newsgroups: bionet.mycology Subject: potato newsletter Date: 5 Oct 1994 19:38:17 -0400 Organization: PEINet, Charlottetown, Prince Edward Island, Canada Lines: 291 Message-ID: <36v9sq$mso@bud.peinet.pe.ca> NNTP-Posting-Host: bud.peinet.pe.ca A new Newsletter that may interest you. Feel free to subscribe or comment. PRINCE EDWARD ISLAND POTATO NEWSLETTER October 1994 Volume 1, No. 2 This is a newsletter to provide information on extension technology that may be useful to the agriculture sector for potato production. The information will provide ideas and information useful to potato production in the North Eastern parts of North America. As this is a Canadian potato newsletter, the information on products will be those registered for use in Canada. Foreign products will be mentioned to provide the agriculture sector with new technology. Please feel free to read the articles and comment on the content. The Newsletter is designed to help individuals to stay up dated on events and technology. Only through comments will the newsletter be able to provide information that will suit your needs. ---------------------------------------------------------------------------- This issue of the Potato Newsletter will focus on Potato Storage Buildings, Post Harvest Potato Treatment, Potato Seed Field Inspection Standards, Items Wanted To Purchase, Products and Services For Sale. ----------------------------------------------------------------------------- POTATO STORAGE BUILDING: Potato storage buildings should be thoroughly inspected to ensure the potato stores well. The storage building must be able to protect the tubers from freezing while held at low temperatures. The building must be capable of maintaining high levels of humidity to keep the potato from loosing moisture. Because the tuber is "living", the storage must be able to move air and the humidity through the piled tubers. Storage buildings should be inspected each year. What should you check for? Here are a few things to check: 1. Insulation - Insulation can be damaged over the years. If they are not kept under control, rodents can cause major damage to the insulation. Therefore check the storage doors, ceilings and walls to ensure all of the insulation is still intact. Keep in mind that the walls of the storage building should have an insulation factor of R-30 while the ceiling should have an insulation factor of R-40. This level of insulation will keep condensation from collecting on your walls and ceilings. Moisture collecting on the building and falling down onto tubers creates ideal conditions for bacterial soft rot to develop. 2. Vapour Barrier - Check the vapour barrier covering the insulation. With high humidity requirements to store potatoes at, the vapour barrier protects moisture from entering the insulation. If moisture enters the insulation, the insulation ability of the potato storage will be reduced. Make sure the vapour barrier is still in good repair to keep moisture from soaking into the storage insulation. 3. Equipment (Fans & Humidifiers) - High quality storage buildings use fans to circulate air through the stored potato tubers. Stored tubers must have frequent air exchanges to remove heat, carbon dioxide, and excess water vapour. Fans must be in top working order to ensure even temperature and water vapour is evenly distributed throughout the piled potato tubers. Humidifiers may be required to ensure the relative humidity remains between 90-95%. Each year always consider if the fans and humidifiers are matched to meet the greatest demand the storage will be faced with. Will there be more tubers to store this year than last year? If the answer is yes, will the equipment handle the extra harvested tubers this year. Will the fan capacity deliver one (1) cubic foot of air per minute per cwt (hundred weight) of tubers. This should be your minimum fan capacity in your storage building. Make sure all equipment is in good repair. Check bearings, belts and lubricate the equipment before filling the storage. When the potato storage building is filled, make sure that the storage is moving air uniformly throughout the building. A good potato storage building must remove excess condensation from the ceilings and walls. 4. Duct System - The duct system that the fans and humidifiers are connected to should be checked. Check to ensure that none of the ducts are plugged or broken. This can be very crucial to ensure air movement is well distributed through the piled potato tubers. Once the potatoes are placed in storage it becomes extremely difficult to repair or clean a duct system. Keep in mind that the duct system has to be properly sized to the fan and humidifier system to ensure proper air flow through the stored potato tubers. 5. Doors - This is one area that causes many producers problems. Storage building doors must function the same as the walls of the building. They require the same insulation and vapour barriers as the walls have. When the doors are closed, they must seal "tightly". There is more heat lost in storage buildings because of doors not closing tight and providing a good quality seal. Doors are very prone to damage. Trucks, tractors and unloader have caused what would be consider small damage but results in major storage losses such as frozen tubers. Check and keep doors in top repair. 6. Control Panel - This is the "brains" of the storage. If the control panel is not functioning properly, the whole storage could be quickly lost. The control panel is connected to thermocouple which should be checked carefully to ensure they are in good repair and are working properly. Be sure that the thermocouple are placed properly in the storage building and replace andy malfunctioned ones. Before filling the storage make sure the control panel operates the dampers and damper motors to ensure the inside environment will be maintained at the levels required to store the harvested tubers. POST HARVEST POTATO TREATMENT: Potato harvesting usually brings with it the risk of storage diseases. To keep these diseases well under control a potato tuber treatment is recommended. MERTECT (thiabendazole) is the standard treatment to consider for treating tubers going into storage. Mertect controls tuber diseases: Dry Rot (Fusarium spp.), Pocket Rot (Phoma spp.), Silver Scurf (Helminthosporium spp.), Skin Spot (Oospora spp.) and Black Scurf (Rhizoctonia spp.). Mertect will provide a higher quality potato to be marketed as table stock. Through the use of Mertect seed potato producers will be able to preserve potato seed vigour, reduce seed decay and keep the five diseases mentioned above under control. The processing potato will benefit from Mertect. Processing potatoes are stored at a higher temperature than seed and table stock tubers. Warm temperatures are more favourable to disease infection than tubers stored at cooler temperatures. Mertect is sold in Canada as a 45% thiabendazole flowable product. When mixed with water, Mertect disperses easily in the water. The product is applied directly to the tubers as a mist as the potato moves over the bin piler. It is also recommended that tubers for seed and table stock be retreated at time of grading to help protect the tubers during shipment and storage at their destination. Mertect Application : 1. Shake the container of Mertect well. 2. Mix 8 litres of Mertect in 170.21 litres of water. Be sure to add the Mertect to the water. Do NOT add water to Mertect. 3. Keep the solution agitated in the holding tank at all times. This prevents the Mertect from settling out. 4. Apply 2 litres of the suspension per metric tonne of potatoes. Be sure the potatoes are rotating over the conveyor line to ensure total tuber coverage. 5. An ideal nozzle pressure for applying the Mertect mist is 80 to 100 psi. This allows a fine mist that will minimize product run-off on the potato tuber. 6. Always try to mix up a new solution of Mertect each day. Do not let the mixture to stand over night. 7. Always follow the labelled directions on the Mertect container. Mertect Applicator: A minimum of equipment is required to apply a Mertect solution. A small motor and pump is needed that will provide 80 to 100 psi of pressure. A holding tank for the Mertect solution with an agitator system, a pressure regulator (with shut-off valve) and a return bypass hose, a pressure gauge and three hollow cone nozzles (8 to 10 inches from the potatoes) that operate on a swivel to set the spray pattern. The applicator system should be installed at a point on the conveyor where the potatoes are falling or turning over. One good place for the spray system is on the bin piler where the potatoes drop onto the boom conveyor form the dirt eliminator or sizing chain. The potatoes both tumble and fall through the mist at this point. The spray equipment can also be watched carefully by the bin piler operator. Be very careful in locating the Mertect treater where the spray mist will cover canvas-backed belts. Canvas belts tend to shrink or separate if they become wet. Use belts that are completely rubber coated. FIELD STANDARDS FOR CLASSES OF SEED POTATOES PRODUCED ON PRINCE EDWARD ISLAND Pre-Elite: During the growing season, the fields are inspected 3 times by Agriculture Canada Potato Inspectors. With each field inspection, the potato plants must be visably free from any varietal mixture and free from visible symptoms of viruses or other diseases that could affect the quality of the seed. Elite I: During the growing season, the fields are inspected 3 times by federal agriculture inspectors. The potato seed fields must be planted in tuber units - whole seed or cut seed. The first 2 inspection have a tolerance for viruses - there must not be any more than 0.1% visible symptoms of viruses. During the last inspection no visible viruses must be observed. For varietal mixture, there is a zero tolerance during each inspection. Elite II: Durinng the growing season, the fields are inspected 3 times by federal agriculture inspectors. The potato seed must be planted in tuber units - whole seed or cut seed. During the first 2 inspections there must be no more than 0.1% visible symptoms of viruses with no symptoms visible during the last inspection. For varietal mixture, there is a zero tolerance during each inspection. Tolerances for field inspections of Elite III, Elite IV, Foundation and Certified are as follows: Elite III: Inspection 1 .25% Total Viruses; .5% total viruses, wilt, blackleg; .1% Foreign Varieties. Inspection 2 .1% Total Viruses; .25% total viruses, wilt, blackleg; 0% Foreign Varieties. Inspection 3 .1% Total Viruses; .25% total viruses, wilt, blackleg; 0% Foreign Varieties. Elite IV: Inspection 1 .5% Total Viruses; 1.0% total viruses, wilt, blackleg; .1% Foreign Varieties. Inspection 2 .1% Total Viruses; .25% total viruses, wilt, blackleg; 0% Foreign Varieties. Foundation: Inspection 1 .5% Total Viruses; 1.0% total viruses, wilt, blackleg; .1% Foreign Varieties. Inspection 2 .2% Total Viruses; .5% total viruses, wilt, blackleg; .05% Foreign Varieties. Certified: Inspection 1 1.0% any one virus; 2.0% Total Viruses; 3% total viruses, wilt, blackleg; 3% Foreign Varieties. Inspection 2 .5% any one virus; 1% Total Viruses; 2% total viruses, wilt, blackleg; .1% Foreign Varieties. IN UP COMING NEWSLETTERS TUBER STANDARDS FOR SEED SHIPMENTS WILL BE DETAILED. ----------------------------------------------------------------------------- WANTED! WANTED! WANTED! WANTED! WANTED! WANTED! WANTED! WANTED! WANTED! Any one wishing to sell Russet Norkotah this year? I have Prince Edward Island buyers who are looking for major sources of this variety. For sales please contact this Newsletter. ----------------------------------------------------------------------------- FOR SALE! FOR SALE! FOR SALE! FOR SALE! I have suppliers of new potato varieties. All seed is Nuclear stock or Pre-Elite. The varieties that are for sale include over 80 potato varieties. These varieties include: ATLANTIC, AC NOVACHIP, AC DOMINO, ALLEGANY, CHIEFTAIN, FONTENOT, GEMCHIP, GOLDRUSH, FRONTIER RUSSET, KATAHDIN, KRANTZ, KENNEBEC, NISKA, NORWIS, REDDALE, RUSSET BURBANK, RUSSET NORKOTAH, SEBAGO, SHEPODY, SNOWDEN, SUPERIOR, YUKON GOLD, D.R. NORLAND, NORCHIP. A hot new variety A7961-1 is also available. For further information on any of these varieties please contact this Newsletter. ----------------------------------------------------------------------------- Genetic Engineering: If you have specific genes in bacteria you wish to introduce into other potato varieties, this newsletter has listings of companies who have the technology and abilities to commercialize this opportunity for you. Confidentiality will be maintained with any genetic requests made by cooperating individuals. ----------------------------------------------------------------------------- If you have products or services that would be of interest to the potato sector, please contact this Newsletter. Comments or suggestions for future Newsletters are welcomed. POTATO NEWS bncraig@bud.peinet.pe.ca From owner-mycology@net.bio.net Tue Oct 04 23:00:00 1994 Path: biosci!PUCCINI.CRL.UMN.EDU!lszabo From: lszabo@PUCCINI.CRL.UMN.EDU (Les J. Szabo) Newsgroups: bionet.mycology Subject: Re: USDA National Needs Fellowships Date: 5 Oct 1994 14:19:55 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 55 Sender: daemon@net.bio.net Distribution: world Message-ID: <54691.lszabo@puccini.crl.umn.edu> NNTP-Posting-Host: net.bio.net We currently have one, possible two open positions for USDA National Needs Fellowships and we are looking for additional canidates. The tilte of the fellowship program is "Towards new and novel plant resistances to diseases caused by biotrophic fungi: molecular gentices of biotrophic fungal pathogens". Our time frame is extremely short, please contact Dr. Zeyen by Oct. 15, 1994 if you are interested. Ph.D. Fellowships in Plant and Fungal Biotechnology Two, three-year Ph.D. student fellowships from the USDA National Needs Graduate Fellowship Program in Plant Biotechnology are available in the Department of Plant Pathology, University of Minnesota, St Paul. Fellowship stipends are $17,000 annually. Fellowship program research involves plant defense systems active against diseases caused by biotrophic fungi. Research areas are: 1.) development of novel resistances for small grained cereals (oats and barley) using transgenics; 2.) genomic mapping of plant disease resistance genes in legumes using RFLP's; 3.) molecular genetics of biotrophic fungi, including mapping of aviulence genes in rust fungi. Fellowships will be filled within the 1994-95 academic year. Each supports a Ph.D. candidate for 3 years. Fellowship appointments can only be held by United States citizens pursuing a full-time Ph.D. program in plant pathology. Fellowship applicants must meet or exceed all University of Minnesota Graduate School requirements. The University of Minnesota is an equal opportunity educator and employer. For information on the University of Minnesota, the Department of Plant Pathology, and graduate school registration materials contact: Professor Richard J. Zeyen Department of Plant Pathology 495 Borlaug Hall University of Minnesota St. Paul, Minnesota 55108 USA Internet: richz@puccini.crl.umn.edu Phone: 612/625-4754 FAX: 612/625-9728 or Les J. Szabo Research Geneticist/Asst. Professor USDA-ARS Cereal Rust Laboratory Department of Plant Pathology University of Minnesota 1551 Lindig St. St. Paul, MN 55108 Phone: (612)625-3780 FAX: (612)649-5054 lszabo@puccini.crl.umn.edu From owner-mycology@net.bio.net Tue Oct 04 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!europa.eng.gtefsd.com!ceylon!news2.near.net!satisfied.elf.com!rpi!vccsouth12.its.rpi.edu!bermaa From: bermaa@vccsouth12.its.rpi.edu (Adam Louis Berman) Newsgroups: bionet.mycology Subject: Kampucha Date: 5 Oct 1994 20:17:51 GMT Organization: Rensselaer Polytechnic Institute, Troy, NY. Lines: 9 Distribution: world Message-ID: <36v1lf$bva@usenet.rpi.edu> Reply-To: bermaa@rpi.edu NNTP-Posting-Host: vccsouth12.its.rpi.edu Is there anyone who can supply information on Kampucha? I am currently researching the reproductional and nutritional capabilities of this organism. If you can offer any help, please reply through email. Thank you From owner-mycology@net.bio.net Tue Oct 04 23:00:00 1994 Path: biosci!biosci!not-for-mail From: vicka@wrq.com (the Littlest Orc) Newsgroups: bionet.mycology,uw.announce,pnw.general,seattle.general Subject: Puget Sound Wild Mushroom Show Date: 5 Oct 1994 08:01:19 -0700 Organization: Walker Richer & Quinn, Inc., Seattle, WA Lines: 26 Sender: kristoff@net.bio.net Distribution: world Message-ID: <36snmu$am3@news.u.washington.edu> NNTP-Posting-Host: net.bio.net Announcing the 31st Annual Puget Sound Mycological Society W I L D M U S H R O O M S H O W !!! Saturday October 22 (noon to 8 PM) and Sunday October 23 (10 AM to 6 PM) at the Center for Urban Horticulture, University of Washington, 3501 41st Street NE, Seattle, Washington There will be over 500 species of wild mushrooms identified and on display, along with demonstrations about cooking, cultivation, ecology, identification, poisonings, arts and crafts, microscopy, and mushroom lore. Fun for everybody, from mushroom maniacs to anyone who's ever wondered about a toadstool! Cost is $4 for general admission, $2 for students and seniors, and free for children under 12. For more information (or general info about the Puget Sound Mycological Society) send email to vicka@wrq.com. --vicka corey vicka@wrq.com "more fun than a barrel of monkey wrenches!" From owner-mycology@net.bio.net Wed Oct 05 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!EU.net!uknet!lyra.csx.cam.ac.uk!warwick!news.shef.ac.uk!silver!mb1jxk From: mb1jxk@silver.shef.ac.uk (J Kennedy) Newsgroups: bionet.mycology Subject: PCR from fungal colonies Date: 6 Oct 1994 12:31:31 GMT Organization: Academic Computing Services, Sheffield University Lines: 10 Message-ID: <370qn3$f8v@hippo.shef.ac.uk> NNTP-Posting-Host: silver.shef.ac.uk X-Newsreader: TIN [version 1.2 PL2] I am going to be doing some PCR on fungal colonies from plates. As I will be screening a lot of colonies (from a cross of Aspergillus nidulans) I am looking for a quick and reliable method. Any suggestions would be welcome. j.kennedy@sheffield.ac.uk From owner-mycology@net.bio.net Wed Oct 05 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!europa.eng.gtefsd.com!swiss.ans.net!newstf01.cr1.aol.com!newsbf01.news.aol.com!not-for-mail From: mycoworld@aol.com (MycoWorld) Newsgroups: bionet.mycology Subject: Wild Matsutake Harvest Date: 5 Oct 1994 23:03:16 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 11 Sender: news@newsbf01.news.aol.com Message-ID: <36vpdk$p70@newsbf01.news.aol.com> NNTP-Posting-Host: newsbf01.news.aol.com I am trying to determine how well the wild matsutake (Tricholoma magnivelare, Armillaria ponderosa, A. Caligata) season is doing around the world. Yields have been poor and prices low here in central Oregon. I have a reliable report from Korea that their season is one of the worst on record. I believe that Canada (BC) is doing ok and hear rumors that China and Russia are doing well - can anyone confirm or deny this info? Also, can anyone tell me what is happening in the Japanese retail market in terms of prices and supply compared to previous years? Thanks for any information. Jerry Haugen, The Mushroom Growers' Newsletter MycoWorld@aol.com From owner-mycology@net.bio.net Wed Oct 05 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!bcm!cs.utexas.edu!swrinde!sgiblab!nbn!news From: harris@bhc.com (Bob Harris) Subject: Fascism at Work in our Government Message-ID: X-Posted-From: InterNews 1.0.4@ncs-23.nbn.com Lines: 19 Sender: harris@miwok.nbn.com Organization: North Bay Network's news posting service - not responsible for content X-Authenticated: harris on POP host miwok.nbn.com Date: Thu, 6 Oct 1994 20:29:24 GMT I just downloaded this and thought all the "mycophiles" might like to investigate this more fully: This may be a bit premature since I have not myself verified exactly what happened, however, I have it on good authority that in mid-to-late-September to DEA sent a written order to ATCC (American Type Culture Center, I believe) demanding that they destroy all their psilocybian mushroom cultures. Evidently the researchers at ATCC carried out the order. It's my understanding that ATCC was one of the only legal sources for such cultures. I'll be trying to get some solid facts on this when I return to on Monday. If anyone has any info please funnel it my way. -richard glen boire The Entheogen Law Reporter P.O. Box 73481 Davis, CA 95617-3481 From owner-mycology@net.bio.net Thu Oct 06 23:00:00 1994 Path: biosci!UMAIL.UMD.EDU!Jerome_J_MOTTA From: Jerome_J_MOTTA@UMAIL.UMD.EDU (jm102) Newsgroups: bionet.mycology Subject: ATCC & Psilocybe Date: 7 Oct 1994 15:39:16 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 22 Sender: daemon@net.bio.net Distribution: world Message-ID: <9410072239.AA18587@umailsrv1.UMD.EDU> NNTP-Posting-Host: net.bio.net Just to set the record straight regarding Psilocybe and ATCC, the fact is that no one ordered, demanded or otherwise pressured ATCC into de-accessioning Psilocybe from its inventory. Psilocybe is on the list of controlled substances. DEA requires a special "License To Distribute" every controlled substance and imposes a significant financial burden of documentation and justification, including licensing, on both the vendor and the vendee. Even though ATCC is a non-profit organization, it is a business and it has to pay the rent. Simply maintaining living cultures is an expensive proposition in and of itself. There isn't much demand from the scientific community for Psilocybe. When ATCC looked at the distribution figures (sales) of Psilocybe cultures and factored in all the additional costs associated with federal regulation, it decided it was just not cost effective to maintain the culture. De-accessioning of Psilocybe was simply a business decision and not the result of coercion by anyone or any agency, government or otherwise. If more of you folks into schroons had bought cultures it would still be available. From owner-mycology@net.bio.net Thu Oct 06 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!swiss.ans.net!newstf01.cr1.aol.com!newsbf01.news.aol.com!not-for-mail From: brianmc@aol.com (BrianMc) Newsgroups: bionet.mycology Subject: Fascism at Work in our Government Date: 7 Oct 1994 17:02:04 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 28 Sender: news@newsbf01.news.aol.com Message-ID: <374d0c$3up@newsbf01.news.aol.com> NNTP-Posting-Host: newsbf01.news.aol.com >I just downloaded this and thought all the "mycophiles" >might like toinvestigate this more fully: > >This may be a bit premature since I have not myself >verified exactly what happened, however, I have it on >good authority that in mid-to-late-September to DEA sent >a written order to ATCC (American Type Culture Center, I >believe) demanding that they destroy all their psilocybian >mushroom cultures. Evidently the researchers at ATCC >carried out the order. It's my understanding that ATCC was >one of the only legal sources for such cultures. > >I'll be trying to get some solid facts on this when I return >to on Monday. If anyone has any info please funnel it my way. Behavior such as this should not be tolerated by the scientific community. There are several species of rare Psilocybe of which only one or two specimens have ever been recorded, the ONLY examples of which now reside in culture collections overseas, and which are now in a very sad state due to the repressive paranoia surrounding the genus, a reputation which it hardly deserves. Are we to not include specimens simply because our govenrment has an excessive fear of the possible psycotrophic content? ---------------------------------------------------------- | Brian McNett e-mail: | Editor: MycoInfo Submissions: | Promoting Mycology in the Online Community From owner-mycology@net.bio.net Thu Oct 06 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!spool.mu.edu!torn!nermal.cs.uoguelph.ca!irwin.cs.uoguelph.ca!lmelvill From: lmelvill@uoguelph.ca (Lewis Melville) Newsgroups: bionet.mycology Subject: Re: Fascism at Work in our Government Date: 7 Oct 1994 15:22:52 GMT Organization: University of Guelph Lines: 29 Message-ID: <373p4c$gtg@nermal.cs.uoguelph.ca> References: NNTP-Posting-Host: irwin.cs.uoguelph.ca X-Newsreader: TIN [version 1.2 PL2] Bob Harris (harris@bhc.com) wrote: : I just downloaded this and thought all the "mycophiles" might like to : investigate this more fully: : This may be a bit premature since I have not myself verified exactly : what happened, however, I have it on good authority that in : mid-to-late-September to DEA sent a written order to ATCC (American : Type : Culture Center, I believe) demanding that they destroy all their : psilocybian mushroom cultures. Evidently the researchers at ATCC : carried out the order. It's my understanding that ATCC was one of the : only legal sources for such cultures. This seems like a futile task. It's highly unlikely that anyone interested in culturing psilocybin for questionable purposes would need to obtain their material from the ATCC. This would be like destroying hemp seed kept in a seed bank as a means to eliminating street use of cannibis. Question: Should scientists carry out this type of order or resort to civil disobedience? : I'll be trying to get some solid facts on this when I return to on : Monday. If anyone has any info please funnel it my way. : -richard glen boire : The Entheogen Law Reporter : P.O. Box 73481 : Davis, CA 95617-3481 From owner-mycology@net.bio.net Thu Oct 06 23:00:00 1994 Path: biosci!rutgers!gatech!swrinde!howland.reston.ans.net!news.sprintlink.net!news.world.net!news.teleport.com!usenet From: "Ralph D. Arnold" Newsgroups: bionet.mycology Subject: Here is An Update Date: Thu, 6 Oct 94 18:50:16 CST Organization: Teleport - Portland's Public Access (503) 220-1016 Lines: 207 Message-ID: <78407.rarnold@teleport.com> Reply-To: NNTP-Posting-Host: ip-c14.teleport.com X-Minuet-Version: Minuet1.0_Beta_16 X-POPMail-Charset: English Several days ago, I posted a news article asking for responses, in order to obtain an EMAIL-based contact list for myco-fans. Below is the latest information I have received. Please update me with additions or changes. The only request is that the organization must be reachable by internet email. Other list can include snail-mail-only groups. My address is at the end of this posting. ////////////////////////////////////////////////////////////////////////// INTERNATIONAL ORGANIZATIONs PUGGEHATTEN-MYCOLOGICAL SOCIETY (Puggehatten is one of the local words for fungi in general. Best translated to "Toads Hat" or something like it.) contact person: Sigvard Svensson email address: Sigvard.Svensson@botmus.lu.se fax number: +46 46 10 42 34 phone number: +46 46 10 40 61 mailing address: Botanical Museum Oestra Vallgatan 18 S-223 61 Lund Sweden description: Local society working with the fungi of SCANIA (Southernmost counties in SWEDEN). We are especially interested in surveying the redlisted species and threatened nemoral biotopes. We are NOT devoted to mycophagy, although we pick the edibles we pass on forays. At the moment we are ca 200 members and issues a "members journal" in swedish, called PUGGEHATTEN with about 100 pages a year divided into four issues. Current annual fee is SEK 75:- *************************************************************************** BRITISH MYCOLOGICAL SOCIETY Contact Person: Dr. S.T. Moss (General Secretary) Email Address: moss@uk.ac.portsmouth.csovax Fax Number: 0705 842070 Phone Number: 0705 842024 Mailing Address: School of Biological Science King Henry Building University of Portsmouth King Henry 1 Street Portsmouth Hants, PO1 2DY, UK Description: Membership is provided for at Associate (Amateur) and Professional levels. A quarterly journal of general mycology - the Mycologist - can be subscribed to without membership of the Society. This journal includes recipes and articles of general interest. Both Associates and Professionals receive the Mycologist as part of their membership package. ////////////////////////////////////////////////////////////////////////// AMERICAN ORGANIZATIONS Fungus Federation of Santa Cruz Contact Person: Nathan Wilson Email Address: nathan@cse.ucsc.edu Phone Number: 408 684-2275 Mailing Address: 1305 East Cliff Dr Santa Cruz, CA 95062 Description: The amateur mushroom group in the Santa Cruz area affiliated with the North American Mycological Association. We meet on the third Wednesday of each month at the Santa Cruz City Museum of Natural History at above mailing address. **************************************************************************** Ohio Mushroom Society Contact Person: Jerry Pepera at gerald.pepera@ab.com Dick Doyle at doyle@cc.denison.edu Fax Number: 216 646-4343 Phone Number: 216 354-4774 (home) 216 646-4277 (work) Mailing Address: 10489 Barchester Dr Concord, OH 44077 Description: Organizes 3-4 mushroom forays per year all over Ohio. Public is always welcome. Evenets feature mushroom taste sampling, workshops, slide show, giveaways, and an organized lunch. Memebrship is $10.00 per year, which includes 6 newsletters, taxonomy service, program materials, and article database (under construiction) ***************************************************************************** MYCOINFO Newsletter Contact Person: brianmc@aol.com Description: This is an interesting newsletter produced free-of-charge via email. To subscribe, send a short message to above email address. ///////////////////////////////////////////////////////////////////////////////// COMMERCIAL ORGANIZATIONS The Mushroom Growers' Newsletter Contact People: Jerry & Trish Haugen Email Address: mycoworld@aol.com Fax Number: 503 883-3273 Phone Number: 503 883-3719 Mailing Address: P.O. Box 5065 Klamath Falls, OR 97601 Description: This monthly (on paper) targets primarily specialty mushroom growers (mostly small-scale growers, hobby growers, or those interested in it). Subjects include: cultivation of various species, marketing info, market prices (San Francisco and New York), environmental control, profiles of commercial growers, production statistics, equipment, book reviews, and sources of information and supplies. ***************************************************************************** Abbey Lane Laboratories Contact Person: Steve Carpenter Email Address: microbe@csos.orst.edu Mailing Address: P.O. Box 1665 Philmath, Oregon Description: Identification of molds for industry and agriculture. Teaching of classes on mushroom ident. in Corvallis ***************************************************************************** Florida Mycology Research Center (FMRC) Contact Person: Stephen L. Peele (curator) Email Address: 72253.1553@compuserve.com Phone Number: 904 327-4378 Mailing Address: P.O. Box 8104 Pensacola, FL 32505 Description: FMRC carries the world's largest mushroom spore and live culture collection. Publishes "The Mushrrom Culture, the Journal of Mushroom Cultivation (TMC)". Sells videos and publications on mushrooms not found elsewhere. Handles four catalogs ($10US each): "MAIN" - growing supplies, spores, and cultures of edible fungi. "RED" - spores and cultures of all types of mushrooms. "CHROMATOGRAPH" - supplies for doing extrations. "PLANT TISSUE CULTURE" - everything for tissue culturing ***************************************************************************** Fungi Perfecti Contact Person: Paul Stamets Email Address: stamets1@aol.com Fax Number: 206 426-9292 Phone Number: 206 426-9377 Mailing Address: P.O. Box 7634 Olympia, WA 98507 Description: FP offers an extremely-wide choice of supplies, books, seminars, accessories, and species/straining for the hobbiest and commercial grower. Contact them for details. ********************************************************************************** Thank you, P.S. I am in no way affiliated with ANY of these or other groups - I'm simply interested in mycology and enjoy the fast contact mode of email. Ralph D. Arnold P.O. Box 889 Oregon City, OR 97045 rarnold@teleport.com From owner-mycology@net.bio.net Fri Oct 07 23:00:00 1994 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!usc!howland.reston.ans.net!torn!nermal.cs.uoguelph.ca!gadwall.cs.uoguelph.ca!sannis From: sannis@uoguelph.ca (Seanna Annis) Newsgroups: bionet.mycology Subject: Re: Leptosphaeria maculans on Arabidopsis Date: 8 Oct 1994 01:53:43 GMT Organization: University of Guelph Lines: 15 Message-ID: <374u37$5i3@nermal.cs.uoguelph.ca> References: <36bpks$787@news.ycc.yale.edu> NNTP-Posting-Host: gadwall.cs.uoguelph.ca X-Newsreader: TIN [version 1.2 PL2] Larry Ilag (llilag@minerva.cis.yale.edu) wrote: : Does anyone know of research articles documenting Leptosphaeria maculans : (Phoma lingam; causal agent of blackleg) infection on Arabidopsis thaliana? : Thanks in advance. : Larry Ilag I am working on this fungus for my PhD. I have never heard of it naturally or artifically attacking Arabidopsis. I suggest getting a few highly virulent cultures and try it. It may work, I don't know off the top of my head how close Arabidopsis is to Crucifers. Dr. Paul Williams, at Michigan State University has a large collection of isolates, and if this host-pathogen interaction has ever been tried he might know. Good luck Seanna sannis@uoguelph.ca From owner-mycology@net.bio.net Fri Oct 07 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!usc!cs.utexas.edu!swrinde!sgiblab!nbn!news From: harris@bhc.com (Bob Harris) Subject: Re: ATCC & Psilocybe Message-ID: X-Posted-From: InterNews 1.0.4@ncs-27.nbn.com Lines: 21 Sender: harris@miwok.nbn.com Organization: North Bay Network's news posting service - not responsible for content References: <9410072239.AA18587@umailsrv1.UMD.EDU> X-Authenticated: harris on POP host miwok.nbn.com Date: Sat, 8 Oct 1994 01:28:03 GMT In article <9410072239.AA18587@umailsrv1.UMD.EDU> Jerome_J_MOTTA@UMAIL.UMD.EDU (jm102) writes: > > Just to set the record straight regarding Psilocybe and ATCC, the fact is > that no one ordered, demanded or otherwise pressured ATCC into de-accessioning > Psilocybe from its inventory. Psilocybe is on the list of controlled > substances. Just to set the record straight: Psilocybe is NOT a controlled substance. Psilocybin and psilocin ARE. The musrhoom has never been controlled under federal law, and only a few states, California being one of them, has ever specified a species of mushroom being illegal. ATCC has always had the requirements that you fill out special forms for these species, but you were still able to order them. As to the business aspect, there are thousands of cultures that ATCC maintains for the genetic diversity reasons alone. Many of those have never sold well. From owner-mycology@net.bio.net Fri Oct 07 23:00:00 1994 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!library.ucla.edu!europa.eng.gtefsd.com!howland.reston.ans.net!spool.mu.edu!torn!nermal.cs.uoguelph.ca!gadwall.cs.uoguelph.ca!sannis From: sannis@uoguelph.ca (Seanna Annis) Newsgroups: bionet.mycology Subject: Re: PCR from fungal colonies Date: 8 Oct 1994 01:59:57 GMT Organization: University of Guelph Lines: 15 Message-ID: <374uet$5i3@nermal.cs.uoguelph.ca> References: <370qn3$f8v@hippo.shef.ac.uk> NNTP-Posting-Host: gadwall.cs.uoguelph.ca X-Newsreader: TIN [version 1.2 PL2] J Kennedy (mb1jxk@silver.shef.ac.uk) wrote: : I am going to be doing some PCR on fungal colonies from : plates. As I will be screening a lot of colonies : (from a cross of Aspergillus nidulans) I am looking for : a quick and reliable method. Any suggestions would be : welcome. : j.kennedy@sheffield.ac.uk : In our lab we have been using a boiling prep method, very little time involved. I believe one of the methods can be found in Biotechniques. I have not used the method myself, but many people in our lab have, and it usually worked well. Good luck. Seanna sannis@uoguelph.ca From owner-mycology@net.bio.net Fri Oct 07 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!agate!howland.reston.ans.net!torn!nntp.cs.ubc.ca!news.UVic.CA!spruce.pfc.forestry.ca!PFC.Forestry.CA!RWINDER From: rwinder@PFC.Forestry.CA (Richard Winder) Subject: Re: Wild Matsutake Harvest Message-ID: <1994Oct7.170502.23027@spruce.pfc.forestry.ca> Sender: news@spruce.pfc.forestry.ca Nntp-Posting-Host: pfc.pfc.forestry.ca Reply-To: rwinder@PFC.Forestry.CA Organization: Forestry Canada (Pacific Forestry Centre) References: <36vpdk$p70@newsbf01.news.aol.com> Date: Fri, 7 Oct 1994 17:05:02 GMT Lines: 21 In article <36vpdk$p70@newsbf01.news.aol.com>, mycoworld@aol.com (MycoWorld) writes: >I am trying to determine how well the wild matsutake (Tricholoma >magnivelare, Armillaria ponderosa, A. Caligata) season is doing around the >world. Yields have been poor and prices low here in central Oregon. I >have a reliable report from Korea that their season is one of the worst on >record. I believe that Canada (BC) is doing ok and hear rumors that China >and Russia are doing well - can anyone confirm or deny this info? I've heard that there really aren't that many in BC this year- a few spots maybe OK due to our diverse geography, but overall the harvest seems to be low according to at least one harvester I talked too. That could just be here on Vancouver Island, though. Prices also seem to be coming down, according to this gentleman, though I can't confirm that- there was somewhat of a gold-rush mentality that is perhaps simmering down. Maybe all the good hunters have staked out their claims and purchased very good flashlights :-) -RSW RICHARD WINDER Title: Research Scientist Canadian Forest Service Phone: (604) 363-0773 Victoria, B.C. Internet: RWINDER@A1.PFC.Forestry.CA From owner-mycology@net.bio.net Sat Oct 08 23:00:00 1994 Path: biosci!MOLBIO.CBS.UMN.EDU!geomay From: geomay@MOLBIO.CBS.UMN.EDU ("Georgiana May") Newsgroups: bionet.mycology Subject: pcr from colonies Date: 9 Oct 1994 13:18:32 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 5 Sender: daemon@net.bio.net Distribution: world Message-ID: <9410092016.AA23243@molbio.cbs.umn.edu> NNTP-Posting-Host: net.bio.net We use Lee's method (BioTechniques 15, Sept.1993). We start with 3 small squares of mycelium, each about 1-1.5 mm sq. and trim away agar underneath. These go into 0-ring Eppendorf tubes with 50 uL extraction buffer. We follow the method and then use 2 uL/25 uL reaction. It comes out about right for Coprinus cinereus, sometimes a 1:10 dilution works better. The mycelium is freshly grown and the squares are removed about 1 cm back from the colony edge. Georgiana May From owner-mycology@net.bio.net Sun Oct 09 23:00:00 1994 Path: biosci!BIO.TAMU.EDU!TOM From: TOM@BIO.TAMU.EDU ("Tom Adams") Newsgroups: bionet.mycology Subject: Mycology FAQ (long) Date: 10 Oct 1994 11:31:14 -0700 Organization: Texas A&M University - Biology Dept Lines: 398 Sender: daemon@net.bio.net Distribution: world Message-ID: Reply-To: Tom@bio.tamu.edu NNTP-Posting-Host: net.bio.net ------------------------------------------------------------------------ The BIONET.Mycology FAQ V 1.0 October 10, 1994 ------------------------------------------------------------------------ This is version 1.0 of the BIONET.MICROBIOLOGY FAQ (frequently asked questions). This FAQ is adapted from one supplied by the MICROBIOLOGY newsgroup and also addresses questions and comments raised by the Mycology newsgroup users. Additional suggestions for future postings should be sent to: tom@bio.tamu.edu leland@straylight.tamu.edu gsmay@bcm.tmc.edu The FAQ will be posted once a month to BIONET.MYCOLOGY, as close to the beginning of the month as possible. The FAQ is organized as follows: I. What is bionet.microbiology II. How to access bionet.microbiology III. How to retrieve old articles IV. How and what to post in bionet.microbiology V. Related newsgroups and bulletin boards VI. Other internet sources of interest to microbiologists VII. Actual frequently asked questions VIII. Miscellaneous I. WHAT IS BIONET.MYCOLOGY ------------------------------- bionet.mycology is an internet newsgroup aimed at the discussion and information exchange of any relevant mycologyical topic. The newsgroupis in the bionet sector and is accessible to subscription via electronicmail and/or directly from newsservers carrying the bionet newsgroups. The newsgroup, bionet.mycology, is intended as a forum for scientific discussions and questions regarding all aspects of the mycological sciences. Topics to be discussed include such diverse aspects of fungal biologyt as genetics, molecular biology, evolution, biochemistry, host-parasite relationships, pathology, physiology, biotechnology, pathogenesis, taxonomy, and the teaching of mycology. The newsgroup also provides a forum for announcing meetings, funding sources, job opportunities, and pointers to other information sources such as the fungal genetics stock center world wide web page. In addition, the newsgroup encourages posting of abstracts for papers that have been accepted for publication in peer reviewed journals. II. HOW TO ACCESS BIONET.MYCOLOGY -------------------------------------- The newsgroup is in the bionet sector and is accessible from your local newsserver (NNTP server), assuming it carries the bionet newsgroups. You also can subscribe to bionet.microbiology via electronic mail. 1) Access by NNTP server The newsgroup is accessible as bionet.mycology by NNTP servers carrying bionet newsgroups. If your NNTP server does not have bionet.mycology, it is likely that your local news administrator has overlooked this newsgroup. Please contact your local news administrator concerning the addition of this newsgroup to your local newsserver (see below). 2) Access by e-mail Everyone in the AMERICAS and the PACIFIC RIM who has no access to a newsserver and wishes to subscribe to bionet.mycology can send an e-mail to: biosci-server@net.bio.net with the following text in the body of the message: subscribe mycology If you are located in EUROPE, AFRICA, OR CENTRAL ASIA and wish to subscribe to bionet.microbiology, send an e-mail to: MXT@dl.ac.uk with the following text in the body of the message: SUB bionet-news.bionet.mycology 3) General BIOSCI/bionet information If you like to know more about e-mail subscription and other bionet.* newsgroups and are located in the AMERICAS or the PACIFIC RIM, send an e-mail from your account to: biosci-server@net.bio.net with info usinfo in the body of your message. If you like to know more about e-mail subscription and other bionet.* newsgroups and are located in Europe, Africa or Asia, send an e-mail from your account to: biosci-server@net.bio.net with info ukinfo in the body of your message. You will receive a file with instructions of how to access the bionet.* newsgroups and furthermore find pointers to other useful information. Dave Kristofferson, the BIOSCI/bionet Manager, and others have done an excellent job in compiling a list of helpful information which are >=a must<= to everybody not familiar with the internet and netnews. This BIOSCI/bionet FAQ will cover all aspects concerning access to newsgroups, subscription, cancellation of subscription, how to post articles, what to post and not to post, how to reply to posts. I strongly recommend everyone not yet familiar with netnews and e-mail subscription to take a few minutes to familiarize themselves with the BIOSCI/bionet FAQ document. The FAQ can be retrieved by sending an e-mail to: biosci-server@net.bio.net with info faq in the body of your message. III. HOW TO RETRIEVE OLD ARTICLES ---------------------------------- 1) Access of bionet.mycology archives by anonymous FTP and gopher Archives for bionet.microbiology can be accessed by anonymous FTP at net.bio.net [134.172.2.69] in the pub/BIOSCI/MYCOLOGY directory. Note that files are ordered by date, and that the filenames are case sensitive. The same archived files are accessible via Gopher using net.bio.net as your gopher server. Gopher also allows you to view the individual messages within each monthly archive file. The files are in the MYCOLOGY directory. Please see the BIOSCI/bionet faq for details (see above). IV. HOW AND WHAT TO POST IN BIONET.MYCOLOGY ------------------------------------------------ Any message or post concerning mycology, or any aspect of interest to the mycology community are welcome in this newsgroup, provided they are scientific in nature or are of scientific interest. Some technical guidelines to posting messages to the bionet newsgroups are described in the BIOSCI/bionet faq (see above), and anybody not certain of how and what to post should become familiar with the document. If you like to post to the newsgroup with your favorite newsreader, please follow the instructions of your newsreader of how to post an article. If you like to post to the newsgroup by e-mail, and are located in the AMERICAS or the PACIFIC RIM, send an e-mail from your account to: mycology@net.bio.net If you like to post to the newsgroup by e-mail, and are located in Europe, Africa or Asia, send an e-mail from your account to: mycology@daresbury.ac.uk V. RELATED NEWSGROUPS AND BULLETIN BOARDS ------------------------------------------ 1) Newsgroups A number of other newsgroups exist in the bionet.* sector which may be of general interest to mycologists. These newsgroups are: bionet.prof-society.ascb bionet.prof-society.faseb bionet.immunology bionet.molbio.evolution bionet.molbio.methds-reagnts bionet.molbio.yeast bionet.mcrobiology bionet.protista bionet.software.www Anybody interested in accessing these newsgroup can do so as described above. VI. OTHER INTERNET SOURCES OF INTEREST ---------------------------------------------------------- Under construction; a few select entries are: 1) e-mail -------- dFLASH server The dFLASH Group has a new electronic mail server that allows GENBANK and PIR similarity searches with the FLASH algorithm. Further information can be obtained by sending: send help in the body of the message to dflash@watson.ibm.com Make sure you have dflash as subject header. 2) Anonymous FTP ---------------- E. coli Database Manfred Kroeger's E. coli datasets are available from the EMBL FTP site ftp.embl-heidelberg.de NIH Forms The ftp site is: ftp.u.washington.edu The directory is: /pub/user-supported/templates 3) Gopher/WAIS/WWW -------------------- ATCC The name of the host computer at the AMERICAN TYPE CULTURE COLLECTION (ATCC) used to access catalogue information has been changed from atcc.org to culture.atcc.org. This change of name affects using gopher to access information describing research materials available from the ATCC. You must point your gopher client to culture.atcc.org (gopher culture.atcc.org). The change also affects using telnet to connect to the ATCC Recombinant Materials Database (clones, vectors, recombinant libraries, transformation hosts, and oligonucleotides) and searching using IRX (telnet culture.atcc.org, username , password ). Candida WWW and gopher severs with information about Candida albicans molecular biology. The servers share most of their text data. The WWW server has additional images and diagrams. To access them: Point your gopher client to: alces.med.umn.edu There is a directory with the Candida information. The URL for the WWW server is: http://alces.med.umn.edu/start.html There are links to the Candida information and other information on the server on that page. Case Western Department of Biochemistry, Case Western Reserve University School of Medicine WWW server with the following URL http://biochemistry.bioc.cwru.edu:80/ E. coli The E. coli genetic stock center has a Web server: http://cgsc.biology.yale.edu/cgsc.html Barbara Bachman's E. coli culture collection can be accessed by gopher under: cgsc.biology.yale.edu This resource can be used more fully if you can hack the SYBASE program, you can telnet directly and do more powerful searches. The CGSC Gopher files are generated periodically from the public portions of the E. coli Genetic Stock Center (CGSC) database. They include information about strains, mutations, genes, and references. We assume that these files will be used primarily for searching for strains with specific mutations or genotype combinations and secondarily for examining stock center information on mutations and genes. The linkage map is being revised, and the list of genes and coordinates in the "Map" file represent the coordinates currently in the database. Access to the "external version" of the CGSC relational database itself is provided either as a menu option (#5) on this gopher (See sub menu item "How to Access..." and "CGSC Database") or by direct telnet to cgsc.biology.yale.edu To gain direct access, you must obtain a password and login as guest. Send e-mail to mary@cgsc.biology.yale.edu to obtain the current password for guest. --Using the CGSC Gopher v. Querying the CGSC Database-- The subset of information contained in the CGSC-gopher file can be accessed by text searches via IUWais. (See IUWAIS search features on the "2. CGSC Files and Database" menu.) You may also directly query the CGSC relational database. If your query is not easily formulated as a simple Boolean search or if a Wais search returns far too many instances to examine one by one, going to the form-based interface of the database will allow you to specify the query in a way that will limit the returns to those of most interest to you. The About file of the submenu provides examples of both types of queries. Read the DBAccess file on the submenu to learn how to navigate between data fields and menu bar and between object-forms. For more detailed instructions, contact Mary at address below for users' guide. Strains bearing CGSC numbers (the only strains present in the flat file) are available from the stock center. The stock center and the database development are supported by the National Science Foundation. For more information about the database or to request strains, contact Mary Berlyn at CGSC: mary@cgsc.biology.yale.edu Fungal Genetics This information source can be found under the following URL: http://kufacts.cc.ukans.edu/cwis/units/fgsc/main.html WWW server at Keck-IBT filamentous fungal databases URL: http://keck.tamu.edu/ibt.html or gopher://keck.tamu.edu Saccharomyces genomic information resource URL: http://genome-www.stanford.edu/ GenBank A searchable GenBank database can be accessed by addressing your gopher client to ftp.bio.indiana.edu, and looking under Genbank-Sequences/ GenomeNet GenomeNet is a Japanese computer network for genome research and related research areas in molecular and cellular biology. It can be accessed by WWW as: http://www.genome.ad.jp Microbiology The WWW Virtual Library >=Microbiology (Biosciences)<= is accessible at: http://golgi.harvard.edu/biopages/micro.html Protist Images Protist Image Data provides pictures and short descriptions of selected protist genera, especially those genera whose species are frequently used as experimental organisms or are important in studies of organismal evolution. The prerelease version of this database can be accessed through the WWW at the following URL: http://megasun.bch.umontreal.ca/protists/protists.html VII. MISCELLANEOUS -------------------- ACKNOWLEDGMENTS ---------------- This FAQ is adapted from a FAQ generated by Martin latterich, University of California, Berkeley. Thanks to Dave Kristofferson and the >=bionetters<= for compiling an excellent and comprehensive BIOSCI/bionet faq, which served as the basis to the technical subscription information in this MYCOLOGY FAQ in sections II. and III. Also many thanks to Keith Robinson, Harvard University, for much information on internet resources and Jim Graham, Indiana, for contributing Barbara Bachman's E. coli culture collection information. ------------------------------------------------------------------------ More information is always welcome. Please mail your suggestions,criticism and information to one of the following Discussion Leaders for bionet.mycology: tom@bio.tamu.edu leland@straylight.tamu.edu gsmay@bcm.tmc.edu Tom Adams Texas A&M University Leland Ellis Texas A&M University Greg May Baylor College of Medicine Tom Adams Department of Biology Texas A&M University College Station, TX 77843 409-845-1468 Tom@bio.tamu.edu From owner-mycology@net.bio.net Mon Oct 10 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!spool.mu.edu!torn!nermal.cs.uoguelph.ca!gadwall.cs.uoguelph.ca!thsiang From: thsiang@uoguelph.ca (Tom Hsiang) Newsgroups: bionet.mycology Subject: AVAILABLE: POST-DOC IN PLANT PATHOLOGY Date: 11 Oct 1994 12:51:39 GMT Organization: University of Guelph Lines: 21 Message-ID: <37e1or$opv@nermal.cs.uoguelph.ca> NNTP-Posting-Host: gadwall.cs.uoguelph.ca X-Newsreader: TIN [version 1.2 PL2] AVAILABLE: POST-DOC IN PLANT PATHOLOGY (October 1994) PLANT PATHOLOGY Post-Doctoral Research Associate. A position is available in the Department of Environmental Biology at the University of Guelph in Ontario. The major project examines the use of a fungal antagonist to control snow mould disease of turfgrass. There are a couple of smaller projects also involving turfgrass fungal pathogens. A good background in field-oriented plant pathology is required. The appointment is available immediately for a 12-month period with possibilities of extension. The salary is $25,000 per year with an additional 13% in benefits which covers prescription drugs, vision care and some supplementary health care benefits. Interested applicants should send a resume (with list of references) and unofficial copies of University transcripts to: Dr. T. Hsiang, Dept. Environ. Biol., Univ. Guelph, Guelph, Ontario, N1G 2W1, Canada. Phone 519-824-4120 ext. 2753. FAX 519-837-0442. E-mail THSIANG@EVBHORT.UOGUELPH.CA. From owner-mycology@net.bio.net Tue Oct 11 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!bcm!news.msfc.nasa.gov!europa.eng.gtefsd.com!newsxfer.itd.umich.edu!gumby!wupost!news.utdallas.edu!corpgate!bcarh189.bnr.ca!nott!cunews!freenet.carleton.ca!FreeNet.Carleton.CA!an524 From: an524@FreeNet.Carleton.CA (Jeffrey Ehlinger) Subject: Help with culture please (newbie) Message-ID: Sender: news@freenet.carleton.ca (Usenet News Admin) Organization: The National Capital FreeNet, Ottawa, Ontario, Canada Date: Wed, 12 Oct 1994 15:44:11 GMT Lines: 18 My professor got some spores for Christmas this past year and he asked me to start them for him. The instructions were in Russian, so we first had to have them translated. The species is "Boletus Edgilus" (the spelling could be wrong). After two weeks, there is many white moldy looking patches on\ the leaf mediumm. I have two trays that are being used. I have lowered the moisture content on one of them to try to get rid of the mold looking stuff. I wonder is this the form the mycelium takes ? Is that what I'm seeing.? In two weeks, I will cut large holes in the plastic bag keeping the moisture in, then hopefully I will get some results. Please e-mail me if you have any idea on how to culture Boletus edgilus. -Jeff PS- the medium should have been completely sterilized when I autocl;aved it at 120C for twenty minutes. From owner-mycology@net.bio.net Tue Oct 11 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!EU.net!Austria.EU.net!newsfeed.ACO.net!swidir.switch.ch!univ-lyon1.fr!jussieu.fr!sxpo.fdn.org!techlink!lionel Message-ID: <36863.142953160@techlink.techlink.fr> Date: 11 Oct 1994 23:52:04 GMT From: lionel@techlink.fr (Lionel Benhamou) Organization: TechnoLink, FirstClass Server Reply-To: lionel@techlink.fr Subject: Myconet Distribution: world Newsgroups: bionet.mycology Lines: 11 I tried two times to join Myconet without success. Am I the only one ? A bientot. lionel@techlink.fr ========================================================================== TechnoLink - FC Server - France | Renseignements : info@techlink.fr (33-1) 49 83 86 49 | Fax :(33-1) 49 83 81 17 ========================================================================== From owner-mycology@net.bio.net Tue Oct 11 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!EU.net!sun4nl!sci.kun.nl!galba.mbfys.kun.nl!hong From: hong@mbfys.kun.nl (Hong Zhou) Subject: Re: Recycled compost Message-ID: Sender: news@sci.kun.nl (News owner) Nntp-Posting-Host: galba.mbfys.kun.nl Organization: University of Nijmegen, The Netherlands References: <35unmj$pvo@terrazzo.lm.com> Date: Wed, 12 Oct 1994 19:51:18 GMT Lines: 6 The logic of fining the company is that they should not pollute the environment by adding even more nitrogen rich substrates to the soil. In Holland we have a law which forbids mushroom farmers to put the wasted substrate on the soil, because the sils are allready heavily polluted with nitrogen from the acid rain. Especially on poor soils there is a richdom of natural plants (and mushrooms!) From owner-mycology@net.bio.net Wed Oct 12 23:00:00 1994 Path: biosci!anthro.umass.edu!etj From: etj@anthro.umass.edu (Eric Jones) Newsgroups: bionet.mycology Subject: subscription info Date: 13 Oct 1994 16:11:17 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HI8LMY8KAQAPUF9G@phobos.ucs.umass.edu> NNTP-Posting-Host: net.bio.net Please send me any information you have on this conference From owner-mycology@net.bio.net Wed Oct 12 23:00:00 1994 Path: biosci!BIO.TAMU.EDU!TOM From: TOM@BIO.TAMU.EDU ("Tom Adams") Newsgroups: bionet.mycology Subject: newsgroup info Date: 13 Oct 1994 12:44:00 -0700 Organization: Texas A&M University - Biology Dept Lines: 398 Sender: daemon@net.bio.net Distribution: world Message-ID: Reply-To: Tom@bio.tamu.edu NNTP-Posting-Host: net.bio.net ------------------------------------------------------------------------ The BIONET.Mycology FAQ V 1.0 October 10, 1994 ------------------------------------------------------------------------ This is version 1.0 of the BIONET.MICROBIOLOGY FAQ (frequently asked questions). This FAQ is adapted from one supplied by the MICROBIOLOGY newsgroup and also addresses questions and comments raised by the Mycology newsgroup users. Additional suggestions for future postings should be sent to: tom@bio.tamu.edu leland@straylight.tamu.edu gsmay@bcm.tmc.edu The FAQ will be posted once a month to BIONET.MYCOLOGY, as close to the beginning of the month as possible. The FAQ is organized as follows: I. What is bionet.microbiology II. How to access bionet.microbiology III. How to retrieve old articles IV. How and what to post in bionet.microbiology V. Related newsgroups and bulletin boards VI. Other internet sources of interest to microbiologists VII. Actual frequently asked questions VIII. Miscellaneous I. WHAT IS BIONET.MYCOLOGY ------------------------------- bionet.mycology is an internet newsgroup aimed at the discussion and information exchange of any relevant mycologyical topic. The newsgroupis in the bionet sector and is accessible to subscription via electronicmail and/or directly from newsservers carrying the bionet newsgroups. The newsgroup, bionet.mycology, is intended as a forum for scientific discussions and questions regarding all aspects of the mycological sciences. Topics to be discussed include such diverse aspects of fungal biologyt as genetics, molecular biology, evolution, biochemistry, host-parasite relationships, pathology, physiology, biotechnology, pathogenesis, taxonomy, and the teaching of mycology. The newsgroup also provides a forum for announcing meetings, funding sources, job opportunities, and pointers to other information sources such as the fungal genetics stock center world wide web page. In addition, the newsgroup encourages posting of abstracts for papers that have been accepted for publication in peer reviewed journals. II. HOW TO ACCESS BIONET.MYCOLOGY -------------------------------------- The newsgroup is in the bionet sector and is accessible from your local newsserver (NNTP server), assuming it carries the bionet newsgroups. You also can subscribe to bionet.microbiology via electronic mail. 1) Access by NNTP server The newsgroup is accessible as bionet.mycology by NNTP servers carrying bionet newsgroups. If your NNTP server does not have bionet.mycology, it is likely that your local news administrator has overlooked this newsgroup. Please contact your local news administrator concerning the addition of this newsgroup to your local newsserver (see below). 2) Access by e-mail Everyone in the AMERICAS and the PACIFIC RIM who has no access to a newsserver and wishes to subscribe to bionet.mycology can send an e-mail to: biosci-server@net.bio.net with the following text in the body of the message: subscribe mycology If you are located in EUROPE, AFRICA, OR CENTRAL ASIA and wish to subscribe to bionet.microbiology, send an e-mail to: MXT@dl.ac.uk with the following text in the body of the message: SUB bionet-news.bionet.mycology 3) General BIOSCI/bionet information If you like to know more about e-mail subscription and other bionet.* newsgroups and are located in the AMERICAS or the PACIFIC RIM, send an e-mail from your account to: biosci-server@net.bio.net with info usinfo in the body of your message. If you like to know more about e-mail subscription and other bionet.* newsgroups and are located in Europe, Africa or Asia, send an e-mail from your account to: biosci-server@net.bio.net with info ukinfo in the body of your message. You will receive a file with instructions of how to access the bionet.* newsgroups and furthermore find pointers to other useful information. Dave Kristofferson, the BIOSCI/bionet Manager, and others have done an excellent job in compiling a list of helpful information which are >=a must<= to everybody not familiar with the internet and netnews. This BIOSCI/bionet FAQ will cover all aspects concerning access to newsgroups, subscription, cancellation of subscription, how to post articles, what to post and not to post, how to reply to posts. I strongly recommend everyone not yet familiar with netnews and e-mail subscription to take a few minutes to familiarize themselves with the BIOSCI/bionet FAQ document. The FAQ can be retrieved by sending an e-mail to: biosci-server@net.bio.net with info faq in the body of your message. III. HOW TO RETRIEVE OLD ARTICLES ---------------------------------- 1) Access of bionet.mycology archives by anonymous FTP and gopher Archives for bionet.microbiology can be accessed by anonymous FTP at net.bio.net [134.172.2.69] in the pub/BIOSCI/MYCOLOGY directory. Note that files are ordered by date, and that the filenames are case sensitive. The same archived files are accessible via Gopher using net.bio.net as your gopher server. Gopher also allows you to view the individual messages within each monthly archive file. The files are in the MYCOLOGY directory. Please see the BIOSCI/bionet faq for details (see above). IV. HOW AND WHAT TO POST IN BIONET.MYCOLOGY ------------------------------------------------ Any message or post concerning mycology, or any aspect of interest to the mycology community are welcome in this newsgroup, provided they are scientific in nature or are of scientific interest. Some technical guidelines to posting messages to the bionet newsgroups are described in the BIOSCI/bionet faq (see above), and anybody not certain of how and what to post should become familiar with the document. If you like to post to the newsgroup with your favorite newsreader, please follow the instructions of your newsreader of how to post an article. If you like to post to the newsgroup by e-mail, and are located in the AMERICAS or the PACIFIC RIM, send an e-mail from your account to: mycology@net.bio.net If you like to post to the newsgroup by e-mail, and are located in Europe, Africa or Asia, send an e-mail from your account to: mycology@daresbury.ac.uk V. RELATED NEWSGROUPS AND BULLETIN BOARDS ------------------------------------------ 1) Newsgroups A number of other newsgroups exist in the bionet.* sector which may be of general interest to mycologists. These newsgroups are: bionet.prof-society.ascb bionet.prof-society.faseb bionet.immunology bionet.molbio.evolution bionet.molbio.methds-reagnts bionet.molbio.yeast bionet.mcrobiology bionet.protista bionet.software.www Anybody interested in accessing these newsgroup can do so as described above. VI. OTHER INTERNET SOURCES OF INTEREST ---------------------------------------------------------- Under construction; a few select entries are: 1) e-mail -------- dFLASH server The dFLASH Group has a new electronic mail server that allows GENBANK and PIR similarity searches with the FLASH algorithm. Further information can be obtained by sending: send help in the body of the message to dflash@watson.ibm.com Make sure you have dflash as subject header. 2) Anonymous FTP ---------------- E. coli Database Manfred Kroeger's E. coli datasets are available from the EMBL FTP site ftp.embl-heidelberg.de NIH Forms The ftp site is: ftp.u.washington.edu The directory is: /pub/user-supported/templates 3) Gopher/WAIS/WWW -------------------- ATCC The name of the host computer at the AMERICAN TYPE CULTURE COLLECTION (ATCC) used to access catalogue information has been changed from atcc.org to culture.atcc.org. This change of name affects using gopher to access information describing research materials available from the ATCC. You must point your gopher client to culture.atcc.org (gopher culture.atcc.org). The change also affects using telnet to connect to the ATCC Recombinant Materials Database (clones, vectors, recombinant libraries, transformation hosts, and oligonucleotides) and searching using IRX (telnet culture.atcc.org, username , password ). Candida WWW and gopher severs with information about Candida albicans molecular biology. The servers share most of their text data. The WWW server has additional images and diagrams. To access them: Point your gopher client to: alces.med.umn.edu There is a directory with the Candida information. The URL for the WWW server is: http://alces.med.umn.edu/start.html There are links to the Candida information and other information on the server on that page. Case Western Department of Biochemistry, Case Western Reserve University School of Medicine WWW server with the following URL http://biochemistry.bioc.cwru.edu:80/ E. coli The E. coli genetic stock center has a Web server: http://cgsc.biology.yale.edu/cgsc.html Barbara Bachman's E. coli culture collection can be accessed by gopher under: cgsc.biology.yale.edu This resource can be used more fully if you can hack the SYBASE program, you can telnet directly and do more powerful searches. The CGSC Gopher files are generated periodically from the public portions of the E. coli Genetic Stock Center (CGSC) database. They include information about strains, mutations, genes, and references. We assume that these files will be used primarily for searching for strains with specific mutations or genotype combinations and secondarily for examining stock center information on mutations and genes. The linkage map is being revised, and the list of genes and coordinates in the "Map" file represent the coordinates currently in the database. Access to the "external version" of the CGSC relational database itself is provided either as a menu option (#5) on this gopher (See sub menu item "How to Access..." and "CGSC Database") or by direct telnet to cgsc.biology.yale.edu To gain direct access, you must obtain a password and login as guest. Send e-mail to mary@cgsc.biology.yale.edu to obtain the current password for guest. --Using the CGSC Gopher v. Querying the CGSC Database-- The subset of information contained in the CGSC-gopher file can be accessed by text searches via IUWais. (See IUWAIS search features on the "2. CGSC Files and Database" menu.) You may also directly query the CGSC relational database. If your query is not easily formulated as a simple Boolean search or if a Wais search returns far too many instances to examine one by one, going to the form-based interface of the database will allow you to specify the query in a way that will limit the returns to those of most interest to you. The About file of the submenu provides examples of both types of queries. Read the DBAccess file on the submenu to learn how to navigate between data fields and menu bar and between object-forms. For more detailed instructions, contact Mary at address below for users' guide. Strains bearing CGSC numbers (the only strains present in the flat file) are available from the stock center. The stock center and the database development are supported by the National Science Foundation. For more information about the database or to request strains, contact Mary Berlyn at CGSC: mary@cgsc.biology.yale.edu Fungal Genetics This information source can be found under the following URL: http://kufacts.cc.ukans.edu/cwis/units/fgsc/main.html WWW server at Keck-IBT filamentous fungal databases URL: http://keck.tamu.edu/ibt.html or gopher://keck.tamu.edu Saccharomyces genomic information resource URL: http://genome-www.stanford.edu/ GenBank A searchable GenBank database can be accessed by addressing your gopher client to ftp.bio.indiana.edu, and looking under Genbank-Sequences/ GenomeNet GenomeNet is a Japanese computer network for genome research and related research areas in molecular and cellular biology. It can be accessed by WWW as: http://www.genome.ad.jp Microbiology The WWW Virtual Library >=Microbiology (Biosciences)<= is accessible at: http://golgi.harvard.edu/biopages/micro.html Protist Images Protist Image Data provides pictures and short descriptions of selected protist genera, especially those genera whose species are frequently used as experimental organisms or are important in studies of organismal evolution. The prerelease version of this database can be accessed through the WWW at the following URL: http://megasun.bch.umontreal.ca/protists/protists.html VII. MISCELLANEOUS -------------------- ACKNOWLEDGMENTS ---------------- This FAQ is adapted from a FAQ generated by Martin latterich, University of California, Berkeley. Thanks to Dave Kristofferson and the >=bionetters<= for compiling an excellent and comprehensive BIOSCI/bionet faq, which served as the basis to the technical subscription information in this MYCOLOGY FAQ in sections II. and III. Also many thanks to Keith Robinson, Harvard University, for much information on internet resources and Jim Graham, Indiana, for contributing Barbara Bachman's E. coli culture collection information. ------------------------------------------------------------------------ More information is always welcome. Please mail your suggestions,criticism and information to one of the following Discussion Leaders for bionet.mycology: tom@bio.tamu.edu leland@straylight.tamu.edu gsmay@bcm.tmc.edu Tom Adams Texas A&M University Leland Ellis Texas A&M University Greg May Baylor College of Medicine Tom Adams Department of Biology Texas A&M University College Station, TX 77843 409-845-1468 Tom@bio.tamu.edu From owner-mycology@net.bio.net Wed Oct 12 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!EU.net!uunet!news.uiowa.edu!franklin.uhl.uiowa.edu!dgaunt From: dgaunt@franklin.uhl.uiowa.edu (Dennis Gaunt) Newsgroups: bionet.mycology Subject: Re: Help with culture please (newbie) Date: 13 Oct 1994 18:44:05 GMT Organization: University of Iowa, Iowa City, IA, USA Lines: 28 Distribution: world Message-ID: <37jv5l$pqk@nexus.uiowa.edu> References: NNTP-Posting-Host: franklin.uhl.uiowa.edu X-Newsreader: TIN [version 1.2 PL2] Jeffrey Ehlinger (an524@FreeNet.Carleton.CA) wrote: : My professor got some spores for Christmas this past year : and he asked me to start them for him. : The instructions were in Russian, so we first had to have them : translated. The species is "Boletus Edgilus" (the spelling could be wrong). : After two weeks, there is many white moldy looking patches on\ : the leaf mediumm. I have two trays that are being used. I have : lowered the moisture content on one of them to try to get rid of : the mold looking stuff. : I wonder is this the form the mycelium takes ? : Is that what I'm seeing.? : In two weeks, I will cut large holes in the plastic bag keeping the : moisture in, then hopefully I will get some results. : Please e-mail me if you have any idea on how to : culture Boletus edgilus. : -Jeff : PS- the medium should have been completely sterilized when I : autocl;aved it at 120C for twenty minutes. Do you mean Boletus edulis? Those white moldy looking patches are probably the fungus that you "planted". -- ---------------------------------------------------------------------- | Dennis D. Gaunt | Internet: dgaunt@uhl.uiowa.edu | | The University of Iowa | Voice: (319) 335-4500 | | Oakdale Research Campus | FAX: (319) 335-4555 | | Iowa City, Iowa 52242 | | ---------------------------------------------------------------------- From owner-mycology@net.bio.net Wed Oct 12 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!pipex!warwick!news.shef.ac.uk!silver!mb935645 From: mb935645@silver.shef.ac.uk (D L Clarke) Newsgroups: bionet.mycology Subject: DNA from dermatophytes Date: 13 Oct 1994 17:18:44 GMT Organization: Academic Computing Services, Sheffield University Lines: 4 Message-ID: <37jq5k$giu@hippo.shef.ac.uk> NNTP-Posting-Host: silver.shef.ac.uk X-Newsreader: TIN [version 1.2 PL2] Does anyone know of a protocol for extraction of DNA from dermatophytes especially Trichophyton and Epidermophyton species. From owner-mycology@net.bio.net Thu Oct 13 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!rutgers!sgigate.sgi.com!olivea!trib.apple.com!amd!netcomsv!netcomsv!netcom.com!netnews From: "Chuck Pullen" Subject: Carbon Monoxide and Fungi Message-ID: <84726.cpullen@netcom15.netcom.com> X-Minuet-Version: Minuet1.0_Beta_16 Sender: netnews@netcom.com (USENET Administration) X-Popmail-Charset: English Reply-To: Organization: NETCOM On-line Communication Services (408 261-4700 guest) Date: Fri, 14 Oct 1994 03:46:00 GMT Lines: 22 I am an industrial hygienist working on a project involving worker protection from carbon monoxide (CO) exposure during comercial fungi agriculture. We have found (CO) concentrations of up to 400 ppm during the growth cycle. CO concentrations correlate with Carbon Dioxide concentrations. Does anyone know a low tempreature biological sourse or process for the generation of CO? I initially thought that spontain. combustion of the compost was the sourse. However, the growth of the fungus is tempreature sensitive. The compost is not allowed to exceed 100 deg. F. which I would think should rule out simple oxidation as a CO sourse. Any help or referals would be appreciated. Chuck Pullen, CIH (cpullen@netcom.com) Pacific Safety Solutions - Sacramento Voice: (916) 687-7993 10215 Davis Road, Suite B Fax: (916) 687-8611 Wilton, CA 95693 "...to seek, to strive, to search, and not to yield." - - Tennyson From owner-mycology@net.bio.net Thu Oct 13 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.moneng.mei.com!uwm.edu!news.doit.wisc.edu!F180-218.net.wisc.edu!han From: han@plantpath.wisc.edu (Shinhan Shiu) Newsgroups: bionet.mycology Subject: Sorry. Date: Fri, 14 Oct 1994 03:26:55 GMT Organization: Dept of Plant Pathology, Univ of Wisconsin-Madison Lines: 2 Message-ID: NNTP-Posting-Host: f180-218.net.wisc.edu In the previous message, it should be "not simply additive but synergistic". From owner-mycology@net.bio.net Thu Oct 13 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!spool.mu.edu!uwm.edu!news.doit.wisc.edu!F180-218.net.wisc.edu!han From: han@plantpath.wisc.edu (Shinhan Shiu) Newsgroups: bionet.mycology Subject: Synergism, hard to define. Date: Fri, 14 Oct 1994 03:21:54 GMT Organization: Dept of Plant Pathology, Univ of Wisconsin-Madison Lines: 21 Message-ID: NNTP-Posting-Host: f180-218.net.wisc.edu I am a first-year grad. And I am going to study the mechanism of synergism between Verticillium dahliae (the potato-early-dying pathogen) and Pratylenchus penetrans (root lesion nematode). According the field data, yeild loss and symptoms tend to be severe when both pathogens are present in the same plot. What we and the other group observe is that the effect is simply additive but synergistic. Before I look into the mechanism of such synergistic effect, I would like to know if there is any well-defined system of synergistic interaction. Please indicate the information source if possible. And, I am tring to send some messages to Prof Carl H. Beckman, Univeristy of Rhode Island, who studies wilt disease of various plants. If anybody know his e-mail add, please inform me about that. Thank you. Shinhan Shiu han@plantpath.wisc.edu Department of Plant Pathology University of Wisconsin-Madison From owner-mycology@net.bio.net Thu Oct 13 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!swrinde!pipex!warwick!news.shef.ac.uk!silver!mb927136 From: mb927136@silver.shef.ac.uk (H Rolph) Newsgroups: bionet.mycology Subject: DNA from dermatophytes Date: 14 Oct 1994 14:48:44 GMT Organization: Academic Computing Services, Sheffield University Lines: 3 Message-ID: <37m5oc$97e@hippo.shef.ac.uk> NNTP-Posting-Host: silver.shef.ac.uk X-Newsreader: TIN [version 1.2 PL2] Does anyone know of a protocol for DNA extraction from dermatophytes (Trichophyton and Epidermatophyton) Helen Rolph H.J.Rolph@sheffield.ac.uk From owner-mycology@net.bio.net Thu Oct 13 23:00:00 1994 Path: biosci!rutgers!uwm.edu!cs.utexas.edu!convex!news.duke.edu!godot.cc.duq.edu!hudson.lm.com!asia.lm.com!not-for-mail From: Richard W. Kerrigan Newsgroups: bionet.mycology Subject: Terminology, homokaryons, pedigrees Date: 14 Oct 1994 07:52:41 -0400 Organization: Telerama Public Access Internet, Pittsburgh, PA Lines: 20 Sender: sylvan@telerama.lm.com Message-ID: <37lre9$q03@asia.lm.com> NNTP-Posting-Host: asia.lm.com X-Newsreader: TIN [version 1.2 PL2] I'm looking for a concise term by which to refer to homokaryons in pedigrees. "The homokaryons that were crossed to produce the F1 hybrid xxx" is a bit cumbersome. I've recently used "progenitor homokaryons" but this does not seem completely satisfactory. More recently, I've seen the term 'midparents' used in this sense. I suspect the term is used by agricultural breeders but the several references I've consulted omit mention of this term. It seems clear and simple. Help, please: (1) What term(s) do you employ for this purpose? (2) What is known about origins and usage of 'midparents'; is it appropriate for this purpose? Thanks in advance. I'll be off-line until 14 November, so please reply or copy your reply to my e-mail address: rwk@sylvanres.com -- Rick Kerrigan Sylvan Research Cabot, PA USA From owner-mycology@net.bio.net Thu Oct 13 23:00:00 1994 Path: biosci!CABI.org!D.BRAYFORD From: D.BRAYFORD@CABI.org ("David Brayford ", IMI) Newsgroups: bionet.mycology Subject: RE: DNA from dermatophytes Date: 14 Oct 1994 03:34:48 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 27 Sender: daemon@net.bio.net Distribution: world Message-ID: <2E9E5F64@smtp-gateway.cgnet.com> NNTP-Posting-Host: net.bio.net I've not actually worked on this, but happen to know of these references: Estruch, J. J., Antuna, C., Ferrer, S. & Ramon, D. (1989). Isolation of genomic DNA from Trichophyton mentagrophytes. Revista Iberica de Micologia 6: 62-66. Davison, F. D. & Mackenzie, D. W. R. ( 1984). DNA homology studies in the taxonomy of dermatophytes. Sabouraudia 22: 117-123. for mtDNA - see Suzuki et al., Mycopathologia 103: 147-151 (1988) and Nishio et al. Mycopathologia 117: 127-132 (1992) Dave Brayford International Mycological Institute UK ---------- From: BIOSCI-REQUEST To: mycology Subject: DNA from dermatophytes Date: 13 October 1994 5:18 Does anyone know of a protocol for extraction of DNA from dermatophytes especially Trichophyton and Epidermophyton species. From owner-mycology@net.bio.net Thu Oct 13 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!europa.eng.gtefsd.com!newsxfer.itd.umich.edu!nntp.cs.ubc.ca!news.UVic.CA!spruce.pfc.forestry.ca!PFC.Forestry.CA!RWINDER From: rwinder@PFC.Forestry.CA (Richard Winder) Subject: Re: Help with culture please (newbie) Message-ID: <1994Oct13.162239.18813@spruce.pfc.forestry.ca> Sender: news@spruce.pfc.forestry.ca Nntp-Posting-Host: pfc.pfc.forestry.ca Reply-To: rwinder@PFC.Forestry.CA Organization: Forestry Canada (Pacific Forestry Centre) References: Date: Thu, 13 Oct 1994 16:22:39 GMT Lines: 42 In article , an524@FreeNet.Carleton.CA (Jeffrey Ehlinger) writes: > > My professor got some spores for Christmas this past year > and he asked me to start them for him. > The instructions were in Russian, so we first had to have them >translated. The species is "Boletus Edgilus" (the spelling could be wrong). Probaby Boletus edulis = cep = steinpilz, etc. = a fungus (basidiomycete) (this fungus forms symbiotic relationships with trees (mycorrhizae) and the sexual fruiting body is a large mushroom with pores, not gills. > After two weeks, there is many white moldy looking patches on\ >the leaf mediumm. I have two trays that are being used. I have >lowered the moisture content on one of them to try to get rid of >the mold looking stuff. > I wonder is this the form the mycelium takes ? Sounds right for a fungus. The mycelium is a mass of thread-like cells which represents the vegetative phase in the life cycle of many fungi. The B. edulis mycelium that I have cultured is white and cottony. Doesn't rule out the possibility of a contaminant, though. > Is that what I'm seeing.? >In two weeks, I will cut large holes in the plastic bag keeping the >moisture in, then hopefully I will get some results. > Please e-mail me if you have any idea on how to >culture Boletus edgilus. It would be nice if you could post the details of the Russian culture method. The only method I've seen reported in the literature involves the use of Hagem/Modess medium (see Pantidou, M. 1961. Cultural studies of Boletaceae. Gyrodon Merulioides and four species of Boletinus. Canadian Journal of Botany 39:1149-1162.) It also grows fairly well, at least at first, on malt extract agar amended with about .1% (or so) yeast extract. I imagine you need a pretty big petri dish to get a fruiting body though :-) -RSW RICHARD WINDER Title: Research Scientist Canadian Forest Service Phone: (604) 363-0773 Victoria, B.C. Internet: RWINDER@A1.PFC.Forestry.CA From owner-mycology@net.bio.net Thu Oct 13 23:00:00 1994 Path: biosci!rnisd0.DNET.roche.com!koenigg From: koenigg@rnisd0.DNET.roche.com Newsgroups: bionet.mycology Subject: PFGE separation of mt DNA? Date: 14 Oct 1994 08:46:55 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <9410141543.AA01620@mailgate.roche.com> NNTP-Posting-Host: net.bio.net Does anyone have a protocol for pulsed field electrophoretic separation of fungal mitochondrial DNA? We are studying the chromosomal arrangements of the human pathogens Coccidioidesimmitis and Histoplasma capsulatum. We would like to apply the right PFGE conditions to separate the mt DNA from the chromosomes. I'd appreciate any references. Thank you. Gina Koenig Roche Molecular Systems Koenigg@rnisd0.dnet.roche.com (510)-814-2815 fax (510) 522-1285 1145 Atlantic Avenue Alameda, CA 94501 USA From owner-mycology@net.bio.net Thu Oct 13 23:00:00 1994 Newsgroups: bionet.mycology Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!wupost!news.utdallas.edu!corpgate!bcarh189.bnr.ca!nott!cunews!freenet.carleton.ca!FreeNet.Carleton.CA!an524 From: an524@FreeNet.Carleton.CA (Jeffrey Ehlinger) Subject: Re: Help with culture please (newbie) Message-ID: Sender: news@freenet.carleton.ca (Usenet News Admin) Organization: The National Capital FreeNet, Ottawa, Ontario, Canada Date: Fri, 14 Oct 1994 05:59:37 GMT Lines: 8 I will look at the patches of moldy -looking stuff. I will look at it under a microscope and look for sporulation. Maybe I can identify it as a beginning of the Boletus edulis or perhaps just penicillin. :( -Jeff PS_ Thanks for all the help folks. From owner-mycology@net.bio.net Thu Oct 13 23:00:00 1994 Path: biosci!MOLBIO.CBS.UMN.EDU!geomay From: geomay@MOLBIO.CBS.UMN.EDU ("Georgiana May") Newsgroups: bionet.mycology Subject: midparents Date: 14 Oct 1994 11:02:44 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: <9410141800.AA17870@molbio.cbs.umn.edu> NNTP-Posting-Host: net.bio.net Rick, I think we should seek to adopt more standard genetic terminology for our crossing experiments. The problem, as I see it, is that we are NOT crossing parents, spores are gametes, the dikaron from which they come are the parents in the sense that breeders use. We just have long-lived gametes which are not available to plant and animal breeders. So, could one scan the literature for what is done in other haploid/diploid systems? What do the yeast breeders (for beer or wine?) do? My only strong feeling is that we shouldn't be calling them parents but probably a lot of people will disagree. Georgiana From owner-mycology@net.bio.net Fri Oct 14 23:00:00 1994 Path: biosci!BADLANDS.NODAK.EDU!jweiland From: jweiland@BADLANDS.NODAK.EDU (John J Weiland) Newsgroups: bionet.mycology Subject: EMBO workshop Filamentous Fungi Date: 15 Oct 1994 09:41:34 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Can anyone relay information to me about the 1995 EMBO workshop on Filamentous Fungi (if it is in 1995)? Where will it be and when? What is the due date for abstracts and how much is registration? THanks! John Weiland jweiland@badlands.nodak.edu From owner-mycology@net.bio.net Fri Oct 14 23:00:00 1994 Path: biosci!BADLANDS.NODAK.EDU!jweiland From: jweiland@BADLANDS.NODAK.EDU (John J Weiland) Newsgroups: bionet.mycology Subject: Manipulations with Filamentous Fungi Date: 15 Oct 1994 08:41:53 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I'm kind of new to these beasts, and am looking for some input into the following questions. The fungus I'm working with is a pathogen of barley called Pyrenophora teres. 1) We currently store long-term cultures of the fungus on silica gel. Is this a standard long term storage media? Can spores be stored in glycerol at -80 degrees C. Can they be stored as stabs in agar (like many bacterial isolates)? 2) Conidia of P. teres can be collected from mycelia grown in culture. Ascospores, however, result from the mating of two opposite mating types of the fungus on barley plants (but not in culture). Has anyone seen mention of mutations in the fungus, or culture conditions, that may induce productive mating of such a fungus in culture, rather than on its normal host plant? 3) Any papers out there on the "bulk segregation analysis" of traits in haploid fungi using PCR? Did Michelmore's group do it with the fungus, or just with the plant traits? Thanks in advance! John Weiland jweiland@badlands.nodak.edu From owner-mycology@net.bio.net Fri Oct 14 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!swiss.ans.net!newstf01.cr1.aol.com!newsbf01.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: Re: ATCC & Psilocybe Date: 15 Oct 1994 15:33:01 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 30 Sender: news@newsbf01.news.aol.com Message-ID: <37papd$s10@newsbf01.news.aol.com> References: NNTP-Posting-Host: newsbf01.news.aol.com In article , harris@bhc.com (Bob Harris) writes: Bob is right.The compounds are illegal; not the genus or its members. Of interest to many of you, I have finally got around to submitting a new species for publication - Psilocybe azurescens which I have co-authored with Jochen Gartz who did extensive analyses. A surprising finding is that when the mycelium of this species, otherwise extremely potent, was cultured on 10% plus malt agar medium (100 grams/liter), there were NO detectable amounts of psilocybin, psilocin, and/or baeocystin! In contrast, mycelia on 2%MEA had, collectively, more than 2.5% (d.w.) of the same. Bizarre, but true.This is the third species I have named and it is by far, the most interesting. It may well be the most potent species ever analyzed. This work was done under the auspices of a DEA license through Dr. Michael Beug of The Evergreen State College and has no connection to Fungi Perfecti. I agree that the loss of these cultures is a loss to us all - especially for future works. paul stamets The article will be published in Integration. The ms. was 20 pages and should clear up or - in the minds of some - further complicate the taxonomy of the Caerulescent species. (No cultures are available. Please do not ask. The type location is Astoria, Oregon.) From owner-mycology@net.bio.net Fri Oct 14 23:00:00 1994 Path: biosci!daresbury!trane.uninett.no!sunic!pipex!uunet!newstf01.cr1.aol.com!newsbf01.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: Re: Kampucha Date: 15 Oct 1994 15:23:04 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 10 Sender: news@newsbf01.news.aol.com Message-ID: <37pa6o$rsa@newsbf01.news.aol.com> References: <36v1lf$bva@usenet.rpi.edu> NNTP-Posting-Host: newsbf01.news.aol.com In article <36v1lf$bva@usenet.rpi.edu>, bermaa@vccsouth12.its.rpi.edu (Adam Louis Berman) writes: I am writing an article for Mushroom, the Journal on Kombucha. I have a fair amount of information - some credible, some quite incredible. Give me your mailing address and I will send you info. on the compounds generated by it. paul stamets From owner-mycology@net.bio.net Sat Oct 15 23:00:00 1994 Path: biosci!agate!spool.mu.edu!howland.reston.ans.net!pipex!warwick!news.shef.ac.uk!silver!mb927136 From: mb927136@silver.shef.ac.uk (H Rolph) Newsgroups: bionet.mycology Subject: h.j.rolph@sheffield.ac.uk Date: 16 Oct 1994 12:22:17 GMT Organization: Academic Computing Services, Sheffield University Lines: 1 Message-ID: <37r5tp$n6e@hippo.shef.ac.uk> NNTP-Posting-Host: silver.shef.ac.uk X-Newsreader: TIN [version 1.2 PL2] dna from dermatophytes responses From owner-mycology@net.bio.net Sat Oct 15 23:00:00 1994 Path: biosci!rutgers!gatech!howland.reston.ans.net!wupost!waikato!waikato.ac.nz!aeiham From: aeiham@waikato.ac.nz Newsgroups: bionet.mycology Subject: Q: soil fungi Peniophora & Pleurotus Message-ID: <1994Oct16.134458.34211@waikato.ac.nz> Date: 16 Oct 94 13:44:58 +1300 Organization: University of Waikato, Hamilton, New Zealand Lines: 8 Can anyone tell me off the top of there head what type of soil fungi _Peniophora gigantea_ and _Pleurotus ostreatus_ are? Just a general description like ecto or VAM mycorrhiza or fungal antagonist similar to Trichoderma or whatever. Many thanks, Terry Brown. From owner-mycology@net.bio.net Sat Oct 15 23:00:00 1994 Path: biosci!daresbury!trane.uninett.no!eunet.no!nuug!EU.net!uunet!utcsri!utnut!torn!uunet.ca!uunet.ca!io.org!prevop.net3.io.org!bpretto From: bpretto@io.org (Bruno Pretto & Paula Vopni) Newsgroups: bionet.mycology Subject: fungus tea Date: Sun, 16 Oct 1994 08:38:21 Organization: Mushrooms/Envirosearch Lines: 3 Message-ID: NNTP-Posting-Host: prevop.net3.io.org Keywords: Kombucha X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Does anybody have any information on verified cases of adverse reactions to consumption of Kombucha tea? Bruno Pretto From owner-mycology@net.bio.net Sun Oct 16 23:00:00 1994 Path: biosci!UCDAVIS.EDU!bmtyler From: bmtyler@UCDAVIS.EDU (Brett Tyler) Newsgroups: bionet.mycology Subject: Re: Manipulations with Filamentous Fungi Date: 17 Oct 1994 08:02:28 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 53 Sender: daemon@net.bio.net Distribution: world Message-ID: <199410171502.IAA14033@bullwinkle.ucdavis.edu> NNTP-Posting-Host: net.bio.net At 8:41 AM 10/15/94 -0700, John J Weiland wrote: >I'm kind of new to these beasts, and am looking for some input into the >following questions. The fungus I'm working with is a pathogen of barley >called Pyrenophora teres. > >1) We currently store long-term cultures of the fungus on silica >gel. Is this a standard long term storage media? Can spores be stored >in glycerol at -80 degrees C. Can they be stored as stabs in agar (like >many bacterial isolates)? > >2) Conidia of P. teres can be collected from mycelia grown in >culture. Ascospores, however, result from the mating of two opposite >mating types of the fungus on barley plants (but not in culture). Has >anyone seen mention of mutations in the fungus, or culture conditions, >that may induce productive mating of such a fungus in culture, rather >than on its normal host plant? > >3) Any papers out there on the "bulk segregation analysis" of traits >in haploid fungi using PCR? Did Michelmore's group do it with the >fungus, or just with the plant traits? > >Thanks in advance! > >John Weiland jweiland@badlands.nodak.edu Regarding 1) In our experience with Neurospora, silica gel is in fact the best long term storage method. Slants or glycerol can be used, depending on the fungus, but can be unreliable. Liquid nitrogen storage is another excellent long term storage method. Regarding 3) We are using bulked segregant analysis for Phytophthora sojae. There is no reason at all why this method can't be used on any genetic progeny showing random segregation of genes. In haploids, BSA should work even better than diploids because dominance and recessiveness (especially of the RAPDs themselves) is not a problem. The most important considerations for BSA are (i) that the trait of interest is determined by a single locus, (ii) that you have at least 8-10 progeny for each bulk (you should have much more than this if the genetics have been done well), (iii) that you are absolutely sure that none of the chosen progeny have been mis-scored and (iv) that there is a sufficient level of DNA sequence polymorphism between the parents (at least 5% of all RAPD bands should differ between the two parents, otherwise you will have a real struggle). Can't help with 2). **************** Brett Tyler Professor bmtyler@ucdavis.edu **************** From owner-mycology@net.bio.net Sun Oct 16 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!pipex!uunet!newstf01.cr1.aol.com!newsbf01.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: Re: fungus tea Date: 17 Oct 1994 10:38:03 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 16 Sender: news@newsbf01.news.aol.com Message-ID: <37u28b$mof@newsbf01.news.aol.com> References: NNTP-Posting-Host: newsbf01.news.aol.com In article , bpretto@io.org (Bruno Pretto & Paula Vopni) writes: Bruno- I have heard that 5 people in Israel died from drinking a contaminated batch of tea. Also, there is a report from Florida which I am tracking down where a woman allegedly died from drinking Kombucha Tea. I have finished writing an extensive article on Kombucha for Mushroom, the Journal which will appear in the next issue. If you can't wait, I'll be glad to send a copy of the ms. (sans photos). How's your cultivation projects? pablo-san From owner-mycology@net.bio.net Sun Oct 16 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!swrinde!pipex!uunet!newstf01.cr1.aol.com!newsbf01.news.aol.com!not-for-mail From: mycoworld@aol.com (MycoWorld) Newsgroups: bionet.mycology Subject: RE:Q: soil fungi Peniophora & Pleurotus Date: 17 Oct 1994 11:54:02 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 15 Sender: news@newsbf01.news.aol.com Message-ID: <37u6mq$nqa@newsbf01.news.aol.com> NNTP-Posting-Host: newsbf01.news.aol.com >aeiham@waikato.ac.nz Writes: >Can anyone tell me off the top of there head what type of soil fungi >_Peniophora gigantea_ and _Pleurotus ostreatus_ are? Pleurotus ostreatus is commonly known as the oyster mushroom. It's widely cutivated - by last report #2 in world production after Agaricus. It's a decayer- grows on tress, straw or just about anything. Peniophora gigantea took me to David Arora's book "Mushrooms Demystified". It's sort of a bracket fungus forming a "paper-thin crust on dead conifers". Apparently a decayer also. Jerry Haugen, The Mushroom Growers' Newsletter MycoWorld@aol.com From owner-mycology@net.bio.net Sun Oct 16 23:00:00 1994 Path: biosci!GNV.IFAS.UFL.EDU!FNM From: FNM@GNV.IFAS.UFL.EDU Newsgroups: bionet.mycology Subject: PFGE of mtDNA Date: 17 Oct 1994 08:49:40 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 32 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HIDRD49ZMQ8XOKGW@gnv.ifas.ufl.edu> NNTP-Posting-Host: net.bio.net Dear Gina, In response to your inquiry about PFGE of mtDNA, you might find the following references helpful. Fukuhara H, Sor F, Drissi R, Dinouel N, Miyakawa I, Rousset S, Viola A-M (1993) Linear mitochondrial DNAs of yeasts: Frequency of occurrence and general features. Mol Cell Biol 13:2309-2314 Han Z, Stachow C (1994) Analysis of Schizosaccharomyces pombe mitochondrial DNA replication by two dimensional gel electrophoresis. Chromosoma 103:162-170 Maleszka R (1993) Single stranded regions in yeast mitochondrial DNA revealed by pulsed field electrophoresis. App Theor Electroph 3:259-263 Maleszka R (1992) Electrophoretic profiles of mitochondrial plasmids in Neurospora suggest they replicate by a rolling circle mechanism. Biochem Biophys Res Commun 186:1669-1673 Maleszka R, Clark-Walker GD (1992) In vivo conformation of mitochondrial DNA in fungi and zoosporic moulds. Curr Genet 22:341-344 Maleszka R, Skelly PJ, Clark-Walker GD (1991) Rolling circle replication of DNA in yeast mitochondria. J EMBO 10:3923-3929 Sincerely, Frank Martin  From owner-mycology@net.bio.net Sun Oct 16 23:00:00 1994 Path: biosci!bmg.bhs.uab.edu!KSROSTAND From: KSROSTAND@bmg.bhs.uab.edu Newsgroups: bionet.mycology Subject: yeast adherence Date: 17 Oct 1994 12:33:57 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Does anyone know a way to block non-specific binding of Candida albicans to plastic? I have tried coating the plastic with albumin, gelatin, and milk proteins(skim milk) but they don't help. I know the yeast does not stick to glass but once the glass is exposed to fetal calf serum ( which it will be in my experiements) the yeast are quite happy to adhere. Thanks. Kathy Rostand, Ph.D. Department of Biochemistry and Molecular Genetics University of Alabama in Birmingham Birmingham, AL 35294 TEL: (205) 934-1967 FAX: (205) 975-2547 e-mail: ksrostand@bmg.bhs.uab.edu From owner-mycology@net.bio.net Sun Oct 16 23:00:00 1994 Path: biosci!rutgers!gatech!swrinde!pipex!uunet!newsflash.concordia.ca!nstn.ns.ca!coranto.ucs.mun.ca!news.unb.ca!nbt.nbnet.nb.ca!news From: Newsgroups: bionet.mycology Subject: Information about mushroom(Armillaria caligata) Date: 17 Oct 1994 17:57:10 GMT Organization: N.B.C.C.- MIRAMICHI Lines: 6 Message-ID: <37udtm$g2o@darwin.nbnet.nb.ca> Reply-To: bthornbu@science.watstar.uwaterloo.u.ca NNTP-Posting-Host: 142.139.182.150 X-Newsreader: WinVN 0.91.3 I am looking for any information concerning the mushroom Armillaria caligata. I am particularly interested in the techniques involved in growing this mushroom. Thanks in advance Robert Mullin From owner-mycology@net.bio.net Sun Oct 16 23:00:00 1994 Path: biosci!NCCCOT.AGR.CA!REDHEADS From: REDHEADS@NCCCOT.AGR.CA Newsgroups: bionet.mycology Subject: Armillaria caligata (R. Mullin) Date: 17 Oct 1994 13:35:33 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HIE1BXDGJ6002IAT@GW.AGR.CA> NNTP-Posting-Host: net.bio.net RE Armillaria caligata Armillaria caligata is really Tricholoma caligatum. It is mycorrhizal and sometimes is said to be a synonym of the Japanese Matsutake, a highly prized edible. Presumably interest stems from that aspect. For those accepting the synonymy there is Japanese literature on attempts to cultivate the mycorrhizal fungus (not necessarily the mushroom fruitbodies). For the true T. caligatum there is less literature. Just what is that you want to know? From owner-mycology@net.bio.net Mon Oct 17 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.cac.psu.edu!news.pop.psu.edu!psuvax1!news.cc.swarthmore.edu!netnews.upenn.edu!dsinc!ub!galileo.cc.rochester.edu!newstand.syr.edu!gamera.syr.edu!griffin From: griffin@gamera.syr.edu (David H. Griffin) Newsgroups: bionet.mycology Subject: midparents Date: 17 Oct 1994 13:14:24 GMT Organization: Syracuse University, Syracuse, NY Science and Forestry Lines: 1 Distribution: world Message-ID: <37ttbg$93@newstand.syr.edu> NNTP-Posting-Host: gamera.syr.edu e-mail: griffin@mailbox.syr.edu From owner-mycology@net.bio.net Mon Oct 17 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!EU.net!uunet!newstf01.cr1.aol.com!newsbf01.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: Re: yeast adherence Date: 17 Oct 1994 20:08:01 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 8 Sender: news@newsbf01.news.aol.com Message-ID: <37v3l1$3m6@newsbf01.news.aol.com> References: NNTP-Posting-Host: newsbf01.news.aol.com In article , KSROSTAND@bmg.bhs.uab.edu writes: Re: Concerning the prevention of yeasts bonding to plastic: Have you tried silicon spray? paul stamets From owner-mycology@net.bio.net Mon Oct 17 23:00:00 1994 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!agate!library.ucla.edu!europa.eng.gtefsd.com!howland.reston.ans.net!pipex!uknet!festival!leeds.ac.uk!bradford.ac.uk!cc117l12.pub.brad.ac.uk!afbrown From: A.F.Brown@bradford.ac.uk (Fred) Newsgroups: bionet.mycology Subject: palaeoethnomycology Q's Date: Tue, 18 Oct 1994 13:22:06 Organization: University of Bradford Lines: 12 Message-ID: NNTP-Posting-Host: cc117l12.pub.brad.ac.uk X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] I am currently wanting to investigate the use of psychoactive fungi in archaic cultures. Any opinions, leads, references, recipes greatly appreciated. I am not another spaced-out hippy with my head in a cloud (of pot smoke), I am trying to understand what has been taboo for a long long time in this culture, and eventually piece together what little research there is in this area. Does Terence Mckenna ring any bells ?.... thanks FRED xxx From owner-mycology@net.bio.net Mon Oct 17 23:00:00 1994 Path: biosci!CABI.org!D.BRAYFORD From: D.BRAYFORD@CABI.org ("David Brayford ", IMI) Newsgroups: bionet.mycology Subject: RE: Manipulations with Filamentous Fungi Date: 18 Oct 1994 04:23:43 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 50 Sender: daemon@net.bio.net Distribution: world Message-ID: <2EA3B0DF@smtp-gateway.cgnet.com> NNTP-Posting-Host: net.bio.net 1. Silica gel is fine for, say, 5 years if you keep it refrigerated. Liquid nitrogen or lyophilisation are better, but obviously more expensive. It is not recommended to keep them growing on agar since they are likely to mutate. 2. I don't know a synthetic medium for crossing this fungus. However, a good ploy is to mimic the natural conditions by pairing isolates on bits of autoclaved barley straw (say 2-3 cm lengths) placed on plain agar. Allow the isolates to grow for 2 weeks to allow colonisation of the straw, then transfer to cool conditions (10 C) for 2-3 weeks, then transfer to 20-25 C in alternating dark and daylight. The fungus usually forms its sexual state in spring after a cold winter snap - so the cool incubation period may help promote perithecial formation - it certainly does in other fungi. Dave Brayford International Mycological Institute d.brayford@cabi.org ---------- From: BIOSCI-REQUEST To: mycology Subject: Manipulations with Filamentous Fungi Date: 15 October 1994 8:41 I'm kind of new to these beasts, and am looking for some input into the following questions. The fungus I'm working with is a pathogen of barley called Pyrenophora teres. 1) We currently store long-term cultures of the fungus on silica gel. Is this a standard long term storage media? Can spores be stored in glycerol at -80 degrees C. Can they be stored as stabs in agar (like many bacterial isolates)? 2) Conidia of P. teres can be collected from mycelia grown in culture. Ascospores, however, result from the mating of two opposite mating types of the fungus on barley plants (but not in culture). Has anyone seen mention of mutations in the fungus, or culture conditions, that may induce productive mating of such a fungus in culture, rather than on its normal host plant? 3) Any papers out there on the "bulk segregation analysis" of traits in haploid fungi using PCR? Did Michelmore's group do it with the fungus, or just with the plant traits? Thanks in advance! John Weiland jweiland@badlands.nodak.edu From owner-mycology@net.bio.net Mon Oct 17 23:00:00 1994 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!agate!howland.reston.ans.net!news.sprintlink.net!news.world.net!news.teleport.com!usenet From: "Ralph D. Arnold" Newsgroups: bionet.mycology Subject: A: Check This Out; B: Help Please Date: Mon, 17 Oct 94 22:49:12 CST Organization: Teleport - Portland's Public Access (503) 220-1016 Lines: 49 Message-ID: <91454.rarnold@teleport.com> Reply-To: NNTP-Posting-Host: ip-c08.teleport.com X-Minuet-Version: Minuet1.0_Beta_16 X-POPMail-Charset: English A: Check This Out: I'm setting up a gopherspace designed to help mushroom cultivators - both hobby and comm'l. Within this gopher is a partial list of commercial sources with internet/email access (major requirement); small list of internet resources (still compiling); files on growing shiitakes (from Mushroompeople) + max. use of autoclaves & cultivating lion's mane (from Mushroom Growers' Newsletter) + excerpts dealing with cultivating fungi from various sources/newsletters. Access to this gopherspace: Via WWW gopher://gopher.teleport.com/11/users/rarnold Via Gopher gopher.teleport.com : select "Users"; then "ON-LINE ACCESS"; then "PLANTS_BY_EMAIL"; then "MUSHROOMS, FUNGI, MYCOLOGY" (This gopherspace is changing almost daily) BTW - if you don't have gopher-ability and you would like to review the material I have collected, simply send me an email and ask for one or more of these subjects (each will be sent as a separate email): Commercial Sources..... Shiitake Article (by MUSHROOMPEOPLE)....... Articles on Autoclave and Cultivating Lion's Mane (by Mushroom Growers'Newsletter)........ EXCERPTS (from various sources)....... FREE Internet Email Materials/Sources B: Help Please : Do you know of commercial sources I might add (they must have internet/email access)? Do you have some text files I might add \ Please send these to me OR some newsletter articles from your \ as part of email message - society I might excerpt THAT deal with / I have trouble with cultivating wild/gourmet fungi ?? / receiving enclosures/files What other suggestions might you have to improve this info-source? Ralph D. Arnold rarnold@teleport.com P.O. Box 889, Oregon City, OR 97045 maintainer of "ON-LINE ACCESS" gopherspace -encouraging internet access gopher://gopher.teleport.com/11/users/rarnold From owner-mycology@net.bio.net Mon Oct 17 23:00:00 1994 Path: biosci!daresbury!trane.uninett.no!sunic!pipex!howland.reston.ans.net!europa.eng.gtefsd.com!newsxfer.itd.umich.edu!nntp.cs.ubc.ca!torn!uunet.ca!uunet.ca!io.org!bonk.io.org!bpretto From: bpretto@bonk.io.org (bruno pretto) Newsgroups: bionet.mycology Subject: medicinal fungi Date: 18 Oct 1994 12:16:28 GMT Organization: Internex Online, Toronto, Ontario, Canada (416 363 3783) Lines: 9 Message-ID: <380eat$kc1@ionews.io.org> NNTP-Posting-Host: bonk.io.org X-Newsreader: TIN [version 1.2 PL2] Does anybody have any information on the use of Inonotus obliquus (Fr.) Pilat, as a medicinal tea. This is a hard polypore and apparently has been used by the Russian community as a medicinal tea for centuries. -- Bruno Pretto. NAMA Trustee. Mycological Society of Toronto. Amateur mycologist. Secretary. Oak Forest Mushroom Association (outdoor shiitake) Paula Vopni. Environmental Research. From owner-mycology@net.bio.net Mon Oct 17 23:00:00 1994 Path: biosci!NCCCOT.AGR.CA!REDHEADS From: REDHEADS@NCCCOT.AGR.CA Newsgroups: bionet.mycology Subject: Edible & Poisonous Mushrooms of Canada Date: 18 Oct 1994 11:54:07 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HIFC1R96JM002U1I@GW.AGR.CA> NNTP-Posting-Host: net.bio.net Excuse me if I am breaking any unwritten rules (and please let me know [politely] if I am as this is all new to me). We have several new copies of "Edible and poisonous mushrooms of Canada" 326 pgs. by J.W. Groves, 1981 printing, available for exchange only, one per person. Is there any interest? Scott Redhead From owner-mycology@net.bio.net Tue Oct 18 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!swrinde!gatech!swiss.ans.net!newstf01.cr1.aol.com!newsbf01.news.aol.com!not-for-mail From: mycoworld@aol.com (MycoWorld) Newsgroups: bionet.mycology Subject: Q: Cortinellus shiitake Date: 18 Oct 1994 23:28:14 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 7 Sender: news@newsbf01.news.aol.com Message-ID: <3823oe$h69@newsbf01.news.aol.com> NNTP-Posting-Host: newsbf01.news.aol.com Is "Cortinellus shiitake" (from a 1979 reference) the same beast as "Lentinula edodes"? Thanks for any feedback. Jerry Haugen, The Mushroom Growrs' Newsletter Mycoworld@aol.com From owner-mycology@net.bio.net Tue Oct 18 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.cac.psu.edu!news.tc.cornell.edu!news.graphics.cornell.edu!newsstand.cit.cornell.edu!NewsWatcher!user From: mab20@cornell.edu (Michael Anthony Ball) Newsgroups: bionet.mycology Subject: DMSO Followup-To: bionet.mycology Date: 19 Oct 1994 13:48:47 GMT Organization: Cornell University/College of Veterinary Medicine Lines: 11 Sender: mab20@cornell.edu (Verified) Message-ID: NNTP-Posting-Host: cu-dialup-0112.cit.cornell.edu Greetings, Can anyone help with references regarding the anti-fungal properities of DMSO?? Is there any data out there that supports its use as an anti-fungal and if so are there any reported MIC values?? Thank-you, M.A. Ball, DVM Department Clinical Science College Veterinary Medicine Cornell University From owner-mycology@net.bio.net Tue Oct 18 23:00:00 1994 Path: biosci!NCCCOT.AGR.CA!REDHEADS From: REDHEADS@NCCCOT.AGR.CA Newsgroups: bionet.mycology Subject: Armillaria caligata (R. Mullin) Date: 19 Oct 1994 10:32:13 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HIGNDBVHOI0031HF@GW.AGR.CA> NNTP-Posting-Host: net.bio.net When I add all your questions up they tell me you are interested in harvesting the Matsutake commercially, and that you are starting from scratch. In North America you should be interested in Tricholoma magnivelare (a.k.a. Armillaria ponderosa, Tricholoma ponderosum), the North American Matsutake (a.k.a. the Pine Mushroom). You are therefore not interested in cultivating sterile mycelium. The Pine Mushroom is not overly common in New Brunswick but probably can be found on well drained sandy or gravelly soils under pines (and possibly hemlocks). If you find some let me know. Researching the literature could be a major task. I suggest you go to a research library and ask to use Biosis or some other source. Again I must ask what is it you are ultimately attempting to do and where are you starting? From owner-mycology@net.bio.net Tue Oct 18 23:00:00 1994 Path: biosci!rutgers!gatech!howland.reston.ans.net!pipex!uunet!newsflash.concordia.ca!nstn.ns.ca!coranto.ucs.mun.ca!news.unb.ca!nbt.nbnet.nb.ca!news From: R. Mullin@nbnet.nb.ca (Robert Mullin) Newsgroups: bionet.mycology Subject: Re: Armillaria caligata (R. Mullin) Date: 19 Oct 1994 15:00:53 GMT Organization: N.B.C.C.- MIRAMICHI Lines: 24 Message-ID: <383cb5$c2d@darwin.nbnet.nb.ca> References: <01HIE1BXDGJ6002IAT@GW.AGR.CA> NNTP-Posting-Host: 142.139.182.155 X-Newsreader: WinVN 0.91.3 In article <01HIE1BXDGJ6002IAT@GW.AGR.CA>, REDHEADS@NCCCOT.AGR.CA says: > >RE Armillaria caligata > >Armillaria caligata is really Tricholoma caligatum. It is >mycorrhizal and sometimes is said to be a synonym of the >Japanese Matsutake, a highly prized edible. Presumably >interest stems from that aspect. For those accepting the >synonymy there is Japanese literature on attempts to >cultivate the mycorrhizal fungus (not necessarily the >mushroom fruitbodies). For the true T. caligatum there is >less literature. Just what is that you want to know? Thank you for your reply. I am interested in the following: What is the difference between T. caligatum and the Japanese Matsutake? How would I get the Japanese literature? How would I cultivate T. caligatum? What conditions and habitat produce optimum growth of T. caligatum? Where would I find T. caligatum in New Brunswick? Your reply would be greatly appreciated. R. Mullin From owner-mycology@net.bio.net Tue Oct 18 23:00:00 1994 Path: biosci!rutgers!gatech!howland.reston.ans.net!EU.net!ub4b!idefix.CS.kuleuven.ac.be!news.sri.ucl.ac.be!NewsWatcher!user From: Moulliard@mbla.ucl.ac.be (Moulliard) Newsgroups: bionet.mycology Subject: MUCL vcancy Date: Wed, 19 Oct 1994 15:53:02 +0100 Organization: MUCL Lines: 46 Distribution: world Message-ID: NNTP-Posting-Host: 130.104.82.1 > FACULTE DES SCIENCES AGRONOMIQUES > UNIVERSITE CATHOLIQUE DE LOUVAIN (UCL) > Louvain-la-Neuve, Belgium. > > At the College of Agriculture of the Catholic University of Louvain, a full > time faculty position has become available: > Field: Molecular biology of microorganisms > Aim: to develop a research group devoted to molecular biology applied to > fungi and bacteria and to supervise the mycotheque of the university > (23 000 strains) with the development and use of molecu