From owner-mycology@net.bio.net Wed Feb 01 22:00:00 1995 Path: biosci!daresbury!bioftp.unibas.ch!citi2.fr!jussieu.fr!news.oleane.net!oleane!pipex!uunet!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: hfromageot@aol.com (HFromageot) Newsgroups: bionet.mycology Subject: Re: Wood Pulp Delignification Using Fungi Date: 1 Feb 1995 18:50:32 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 6 Sender: root@newsbf02.news.aol.com Message-ID: <3gp6o8$ods@newsbf02.news.aol.com> References: <3gn2no$e5a@usenetw1.news.prodigy.com> Reply-To: hfromageot@aol.com (HFromageot) NNTP-Posting-Host: newsbf02.mail.aol.com Yes, I heard of various mediators involved in laccase-oxygen oxidation of lignin among other substrates. The German inventor, Dr. H.-P. Call, mentioned recently that such mediators belonged to any of the following four classes of organic compounds: N-oxides, N-hydroxides, oximes and hydroxamic acids. I am really interested to know whether anyone else found similar results. Please keep me posted. From owner-mycology@net.bio.net Wed Feb 01 22:00:00 1995 Path: biosci!adam.cc.sunysb.edu!news.sprintlink.net!howland.reston.ans.net!torn!ccshst05.cs.uoguelph.ca!ccshst01.cs.uoguelph.ca!lmelvill From: lmelvill@uoguelph.ca (Lewis Melville) Newsgroups: bionet.mycology Subject: Re: mycorhizal fungi Date: 2 Feb 1995 21:20:07 GMT Organization: University of Guelph Lines: 21 Distribution: world Message-ID: <3gria7$lj2@ccshst05.cs.uoguelph.ca> References: <3g5juu$8rf@bud.peinet.pe.ca> NNTP-Posting-Host: ccshst01.cs.uoguelph.ca X-Newsreader: TIN [version 1.2 PL2] Paul Stewart (stewart@bud.peinet.pe.ca) wrote: : Fellow Myconuts: : Has anyone a contact for research or info regarding mycorhizal fungi? : Specifically, I'm looking for any attempts to culture them. My idea is : to try extracts of the companion roots or surrounding soil as additives : to agar and similar additives to the spawn mix (grain, wood chips, sawdust). We published a book called Practical Methods in Mycorrhiza Research which contains information on culturing and isolating Ecto fungi. It is available from Mycologue PUblications, 8728 Lochside Drive, Sidney, B.C. Canada. V8L 1M8. U.S. $23.00. Culturing VAM fungi is a different story, usually done in pot cultures with a host plant. You could also join the micronet listserv list, and contact mycorrhiza reseachers that way. SEnd a mail message to listserv@uoguelph.ca with the message subscribe micronet Good luck. lewis melville. From owner-mycology@net.bio.net Wed Feb 01 22:00:00 1995 Newsgroups: bionet.mycology Path: biosci!adam.cc.sunysb.edu!news.sprintlink.net!howland.reston.ans.net!gatech!newsxfer.itd.umich.edu!nntp.cs.ubc.ca!news.UVic.CA!spruce.pfc.forestry.ca!news.pfc.forestry.ca!PFC.Forestry.CA!RWINDER From: rwinder@PFC.Forestry.CA (Richard Winder) Subject: Growing mushrooms on Internet X-Nntp-Posting-Host: pfc.pfc.forestry.ca Message-ID: Sender: news@news.pfc.forestry.ca (0000-news(0000)) Reply-To: rwinder@PFC.Forestry.CA Organization: Forestry Canada (Pacific Forestry Centre) Date: Wed, 1 Feb 1995 20:34:23 GMT Lines: 88 Dear mushroom fans, For those who have been following the mushroom cultivation thread, I just spied the following in rec.gardens. Subject: MUSHROOM GROWING (hobby & comm'l) Message-ID: <57380.rarnold@teleport.com> From: "Ralph D. Arnold" Date: Sat, 28 Jan 95 12:25:19 PST Reply-To: Organization: Teleport - Portland's Public Access (503) 220-1016 Do you enjoy eating mushrooms, especially gourmet/wild ones? Would you like to learn how to grow them for fun, for profit(!), or ecology (recycling)?? Perhaps this will interest you? You can now subscribe to FUNGUS, a free Internet mailing list dedicated to mushroom cultivation (both hobby and commercial) and related mycology resources. I. Philosophical Statement about FUNGUS FUNGUS has been designed as a vehicle for mushroom growers (both hobbyists and professionals) and interested parties can have one more Internet access point, for dissemination of information and for discussions relating to the cultivation of edible and other fungi. Conceiveable, there will be a variety of commercial entities publicized and involved. This is meant to be a practical approach to cultivation. II. Important Non-Internet Resources for the Mushroom Grower This FREE Internet tool is IN NO WAY meant to replace/supplant other resources. I suggest all growers utilize this partial list of resources B. How to Unsubscribe to FUNGUS Send an email message to fungus-request@teleport.com with this message: UNSUBSCRIBE FUNGUS .. V. Any questions/comments can be directed to the moderator, which is Ralph D. Arnold. He can be reached at rarnold@teleport.com. RICHARD WINDER Title: Research Scientist Canadian Forest Service Phone: (604) 363-0773 Victoria, B.C. Internet: RWINDER@A1.PFC.Forestry.CA From owner-mycology@net.bio.net Wed Feb 01 22:00:00 1995 Path: biosci!hubcap.clemson.edu!biosci!D.UMN.EDU!grad From: grad@D.UMN.EDU (stephen hedman) Newsgroups: bionet.mycology Subject: Position Announcement Date: 30 Jan 1995 15:20:01 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 27 Sender: daemon@net.bio.net Distribution: world Message-ID: <199501302319.RAA14554@ub.d.umn.edu> NNTP-Posting-Host: net.bio.net Cell Biologist The University of Minnesota-Duluth Department of Biology invites applications for a tenure-track Assistant Professor position beginning September 1, 1995. The position requires a Ph.D in a biological science and postdoctoral research experience. Responsibilities will include: teaching cell biology, introductory biology, and advanced specialty courses; undergraduate advisement; graduate education (M.S. emphasis); and establishment of a strong extramurally funded research program. Collaborative research with faculty in one of three University of Minnesota, Duluth School of Medicine departments (Anatomy and Cell Biology, Medical Microbiology and Immunology, Medical and Molecular Physiology) strongly encouraged. Send curriculum vitae, copies of undergraduate and graduate transcripts, statement of teaching and research interests, up to three reprints, and three letters of recommendation that address teaching and research potential to: Dr. Conrad E. Firling, Cell Biology Search Chair, Department of Biology, University of Minnesota, 10 University Drive, Duluth, Minnesota 55812. Application deadline, March 20, 1995. The University of Minnesota is an Equal Opportunity Educator and Employer. Contact: Conrad E. Firling, email: cfirling@ub.d.umn.edu; TEL: 218-726-7270; FAX:218-726-8142 From owner-mycology@net.bio.net Wed Feb 01 22:00:00 1995 Path: biosci!NCCCOT.AGR.CA!SEIFERTK From: SEIFERTK@NCCCOT.AGR.CA Newsgroups: bionet.mycology Subject: Biological delignification of pulp Date: 1 Feb 1995 11:05:21 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 43 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HMJD8K44F6003AS1@GW.AGR.CA> The recent post on the use of white rot fungi to delignify pulp lead me to contact a 'ringer' to get a good answer... Delignification of pulps with enzymes is an active research area, lying somewhere between a scientific curiosity and a practical process. The use of xylanase to aid delignification and decrease (by ca. 25%) the amounts of chemicals required for bleaching is well established in Scandinavian and Canadian mills. Several large enzyme suppliers produce xylanase for the pulp bleaching market, often from Trichoderma, and competition to improve enzyme properties and lower price is intense. The use of oxidative enzymes from white-rot fungi that can directly attack lignin is a second generation approach, which could produce larger chemical savings than xylanase, but has not yet been developed to the mill scale. It is being studied at the Pulp and Paper Research Institute of Canada, several laboratories in Japan, and at Lignozym, a private research company in Germany. Enzyme and chemical suppliers are keenly interested, and there is likely more research going on but not yet publicized. Certain white- rot fungi can delignify kraft pulps, increasing their brightness and their responsiveness to brightening with chemicals. The fungal treatments are too slow, but the enzymes manganese peroxidase and laccase can also delignify pulps, and enzymatic processes are likely to be easier to optimize and apply than the fungal treatments. Progress up to 1993 was reviewed by Reid and Paice (FEMS Microbiology Reviews 13 (1994) 369-376). Pulp delignification with laccase is enhanced by the presence of a compound named ABTS, by an unknown mechanism. In the spring of 1994, Hans-Peter Call of Lignozym announced the discovery of a superior, but unnamed, "mediator" that also enhanced the action of laccase on pulp, allowing 66% lignin removal. For the rest of 1994, the grapevine buzzed with speculations about the identity of Call's mediator. His patent application has just been published (WO 94/29510), and we now know that the mediator is hydroxybenztriazole (HBT). Call has reported successful pilot plant trials, but there are concerns that HBT is too expensive for an economical process. Development work on laccase- mediator systems and on manganese peroxidase continues; further advances will probably be reported at the International Conference on Biotechnology in the Pulp and Paper Industry in Vienna this June. Ian D. Reid, Pulp and Paper Research Institute of Canada (PAPRICAN) REID@paprican.ca From owner-mycology@net.bio.net Wed Feb 01 22:00:00 1995 Path: biosci!NEXTLAB.CALSTATELA.EDU!spencal From: spencal@NEXTLAB.CALSTATELA.EDU (Steve Pencall) Newsgroups: bionet.mycology Subject: LA MUSHROOM FAIR Date: 1 Feb 1995 17:30:55 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 61 Sender: daemon@net.bio.net Distribution: world Message-ID: <9502020122.AA15693@nextlab.calstatela.edu> NNTP-Posting-Host: net.bio.net Wild Mushroom Fair, February 19, 1995 With this year's heavy rains, a bumper crop of mushrooms is assured in nearby fields and forests. To celebrate this bounty, the Los Angeles Mycological Society will be staging a Wild Mushroom Fair at the Los Angeles State and County Arboretum, 301 North Baldwin Avenue, Arcadia, California on Sunday, February 19, 1995 from 9:00 a.m. to 4:30 p.m. Admission is free with admission to the Arboretum ($3.00). The Fair will feature identified species of Wild Mushrooms displayed in natural habitat settings. Mushroom experts will be on hand to answer all your questions about Wild Mushrooms and you are invited to bring in Wild Mushrooms for identification. A variety of Mushroom books and posters will be offered for sale. The featured speaker will be Dr. Chester R. Leathers, retired professor of botany at Arizona State University in Tempe. Dr. Leathers will deliver an illustrated talk on "An Introduction to Mushrooms and Their Identification". On Saturday, February 18, there will be 3 Mushroom walks, or forays, to collect Wild Mushrooms for display at the Fair. One foray will be at Malibu Creek State Park, one at Placerita Canyon County Park and one in the Cleveland National Forest east of Orange County. The public is invited to participate in these forays free of charge. Please call the Los Angeles Mycological Society at (213) 292-1900 for up to date information on the Mushroom forays and Wild Mushroom Fair. Wild Mushroom Fair, February 19, 1995 With this year's heavy rains, a bumper crop of mushrooms is assured in nearby fields and forests. To celebrate this bounty, the Los Angeles Mycological Society will be staging a Wild Mushroom Fair at the Los Angeles State and County Arboretum, 301 North Baldwin Avenue, Arcadia, California on Sunday, February 19, 1995 from 9:00 a.m. to 4:30 p.m. Admission is free with admission to the Arboretum ($3.00). The Fair will feature identified species of Wild Mushrooms displayed in natural habitat settings. Mushroom experts will be on hand to answer all your questions about Wild Mushrooms and you are invited to bring in Wild Mushrooms for identification. A variety of Mushroom books and posters will be offered for sale. The featured speaker will be Dr. Chester R. Leathers, retired professor of botany at Arizona State University in Tempe. Dr. Leathers will deliver an illustrated talk on "An Introduction to Mushrooms and Their Identification". On Saturday, February 18, there will be 3 Mushroom walks, or forays, to collect Wild Mushrooms for display at the Fair. One foray will be at Malibu Creek State Park, one at Placerita Canyon County Park and one in the Cleveland National Forest east of Orange County. The public is invited to participate in these forays free of charge. Please call the Los Angeles Mycological Society at (213) 292-1900 for up to date information on the Mushroom forays and Wild Mushroom Fair. From owner-mycology@net.bio.net Wed Feb 01 22:00:00 1995 Path: biosci!NEXTLAB.CALSTATELA.EDU!spencal From: spencal@NEXTLAB.CALSTATELA.EDU (Steve Pencall) Newsgroups: bionet.mycology Subject: LA MUSHROOM FAIR Date: 1 Feb 1995 17:27:41 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: <9502020119.AA15675@nextlab.calstatela.edu> NNTP-Posting-Host: net.bio.net Wild Mushroom Fair, February 19, 1995 With this year's heavy rains, a bumper crop of mushrooms is assured in nearby fields and forests. To celebrate this bounty, the Los Angeles Mycological Society will be staging a Wild Mushroom Fair at the Los Angeles State and County Arboretum, 301 North Baldwin Avenue, Arcadia, California on Sunday, February 19, 1995 from 9:00 a.m. to 4:30 p.m. Admission is free with admission to the Arboretum ($3.00). The Fair will feature identified species of Wild Mushrooms displayed in natural habitat settings. Mushroom experts will be on hand to answer all your questions about Wild Mushrooms and you are invited to bring in Wild Mushrooms for identification. A variety of Mushroom books and posters will be offered for sale. The featured speaker will be Dr. Chester R. Leathers, retired professor of botany at Arizona State University in Tempe. Dr. Leathers will deliver an illustrated talk on "An Introduction to Mushrooms and Their Identification". On Saturday, February 18, there will be 3 Mushroom walks, or forays, to collect Wild Mushrooms for display at the Fair. One foray will be at Malibu Creek State Park, one at Placerita Canyon County Park and one in the Cleveland National Forest east of Orange County. The public is invited to participate in these forays free of charge. Please call the Los Angeles Mycological Society at (213) 292-1900 for up to date information on the Mushroom forays and Wild Mushroom Fair. From owner-mycology@net.bio.net Thu Feb 02 22:00:00 1995 Path: biosci!ns1.faseb.org!darwin.sura.net!news.tulane.edu!tulane!ames!onramp.arc.nasa.gov!pioneer.arc.nasa.gov!watson From: watson@pioneer.arc.nasa.gov (John S. Watson - FSC) Newsgroups: bionet.mycology Subject: Honey Mushrooms are Tree killers? Date: 3 Feb 1995 00:05:08 GMT Organization: NASA Ames Research Center Lines: 26 Distribution: na Message-ID: <3grrvk$ltt@onramp.arc.nasa.gov> NNTP-Posting-Host: pioneer.arc.nasa.gov Hello, Recently the brich in front of my parents house all died, and around them were a bunch of small muchrooms that looked like honey mushrooms, ( Armillariella [= Armillaria] mella ). At a friends house near here I recently found much bigger honey mushrooms all around 3 of his tree. Acording to David Aura's book "Mushroom's Demystified", honey mushrooms are parasite for which there is no cure, and which eventually kills the host tree. So does anyone know anymore about these guys? Is there anything that can be done except sit back and enjoy the mushrooms? So, are all these trees doomed? How long does it take to kill them? Should trees killed by them be replaced in the same area, and and should we wait a while to do so? Thanks, John John S. Watson NASA Ames Research Center MS/243-9, Moffett Field, CA 94035 John S. Watson "Don't it always seem to go, that you don't know what you've got 'til it's gone ..." From owner-mycology@net.bio.net Thu Feb 02 22:00:00 1995 Path: biosci!CS.Arizona.EDU!news.Arizona.EDU!hamblin.math.byu.edu!sol.ctr.columbia.edu!newsxfer.itd.umich.edu!gatech!howland.reston.ans.net!agate!news.ucdavis.edu!jerome.ucdavis.edu!user From: jfrigot@ucdavis.edu (Jerome Rigot) Newsgroups: bionet.mycology Subject: fungal-mediated bioremediation research funding Date: Fri, 03 Feb 1995 08:37:57 -0800 Organization: UCDavis Lines: 17 Message-ID: NNTP-Posting-Host: jerome.ucdavis.edu Hello, I am a grad student doing research at UCDavis in soil bioremediation, using soil fungi (not white rot). Unfortunatly, our funding through the EPA Superfund program got cut. I would like to finish my work, and I was wondering if any of you would know of funding sources to which I could apply to request some support for next year (95-96). Any resources would be appreciated concerning private or public funding. E-write to me directly, or post on the newsgroup, whichever you prefer. Thank you, Jerome. -- Jerome Rigot Dept. Environmental Toxicology University of California Davis, CA 95616 (916)752-5896 e-mail: jfrigot@ucdavis.edu From owner-mycology@net.bio.net Thu Feb 02 22:00:00 1995 Newsgroups: bionet.mycology Path: biosci!bcm!cs.utexas.edu!uunet!newsfeed.ACO.net!edvz.sbg.ac.at!DPFL03!tuerk From: tuerk@edvz.sbg.ac.at (Roman Tuerk) Subject: Third IAL Symposium- IAL3 Message-ID: Keywords: Lichen, International Association fro Lichenology, WWW Lines: 90 Sender: news@wst.edvz.sbg.ac.at (USENET News System) Organization: Institut of Plant Physiology - University of Salzburg X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Date: Fri, 3 Feb 1995 11:39:52 GMT Third IAL Symposium: preliminary announcement (online WWW - Informations: http://www.edvz.sbg.ac.at/pfl/ial3/prelimi.htm) Third IAL Symposium - IAL3: preliminary announcement Progress and Problems in Lichenology in the Nineties (Salzburg, Austria 1 - 7 September 1996) Preliminary announcement Arranged by the Intitute of Plant Physiology, University of Salzburg and the International Association for Lichenology Organisation I. Kärnefelt, President R. Türk, Secretary Local organizing Committee and Advisory Board O. Breuss, G. Gärtner, J. Hafellner, H. Mayrhofer, E. Stocker-Wörgötter, J. Üblagger General Information The third international meeting arranged by the IAL and the Insitute of Plant Physiology of the University of Salzburg will be held from 1st to 7th September 1996 at the University of Salzburg, Faculty of Natural Sciences. This symposium continues the series of international meetings on lichen biology which started at Berlin 1969. It is open to any person interested in lichenized fungi or working in fields such as morphology, physiology, ecology, chemistry and systematics of lichens. Preliminary Announcement and Second Circular If you are interested in receiving further information on the symposium please contact the scientific secretary. The second circular will be distributed during May 1995. Language The language at the Congress will be English. Scientific Programme The symposium will include oral contributions and poster sessions. It is planed to avoid simultaneous sessions. The preliminary scientific programme includes nine different sections: A. Systematics B. Morphology C. Ecology D. Chemistry E. Changes of Environment and Conservation F. Resynthesis and Coltivation G. Lichens of High Mountains H. Photobionts I. Lichens in the Tropics Scientific Sessions An estimated 20 minutes, comprising a 15 minutes lecture and a 5 minutes discussion will be allowed for each lecture. Contributed Papers Contributed papers may be accepted either as lectures or as posters depending on the decision of the convenors. Travel and Accomodations Salzburg can be reached by train and by air. There is the possibility to arrive by air in Vienna (Austria) or in Munich (Germany) and take the regional train to Salzburg. All the symposium partecipants can be offered hotel accomodation in modern student dormitories wich are used as hotel during summer time for a reasonable price. Camping accomodation is also available Congress Fee The Congress Fee will be estimated at 2.200.-- ATS (approximately equal to 220.-- $) for full members and 1.100.-- ATS for students. Excursions Four different excursions are offered before and after the Congress. Details will be presented in the second circular. Correspondence All correspondence and inquiries should be adressed to the scientific secretariat: Dr. Roman Türk University of Salzburg, Institute of Plant Physiology Hellbrunnerstr. 34 A - 5020 Salzburg, Austria Telephone Nat.: 0662 8044 5588; 0662 8044 5559 Telephone Int.: +43 662 8044 5588; +43 662 8044 5559 Fax: +43 662 8044 5010 E-mail: tuerk@edvz.sbg.ac.at From owner-mycology@net.bio.net Thu Feb 02 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!prairienet.org!jdkirkla From: jdkirkla@prairienet.org (Justin D. Kirkland) Newsgroups: bionet.mycology Subject: I need Pleurotus slides Date: 3 Feb 1995 04:42:41 GMT Organization: University of Illinois at Urbana Lines: 14 Message-ID: <3gsc81$a3c@vixen.cso.uiuc.edu> NNTP-Posting-Host: firefly.prairienet.org I am a senior at the University of Illinois and an agronomy major. I am enrolled in a required senior seminar class where we basically give presentations to our peers. The idea is to loosen us up for interviews or future job presentations. My main area of interest is mushroom production and would like to give a presentation on Oyster mushroom production. I am familar with the subject---Stamet's basic course, his books, etc... However living in central IL there are no slides available. I would like to borrow, rent, purchase, or trade for slides. I titled my presentation "From Spore to Store" and would appreciate any response. My presentation is Feb 23. Thanks very much--Justin Kirkland -- From owner-mycology@net.bio.net Fri Feb 03 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.cac.psu.edu!news.pop.psu.edu!psuvax1!news.cc.swarthmore.edu!netnews.upenn.edu!wal6000a.udc.upenn.edu!gosfield From: gosfield@udcemail.udc.upenn.edu (Edward Gosfield) Newsgroups: bionet.mycology Subject: southern mushrooms Date: 4 Feb 1995 04:29:31 GMT Organization: University of Pennsylvania Lines: 13 Message-ID: <3guvrb$f1j@netnews.upenn.edu> NNTP-Posting-Host: wal6000a.udc.upenn.edu X-Newsreader: TIN [version 1.2 PL2] latest sales circular from Edward R. Hamilton, Bookseller, Falls Village, CT 06031-5000 offers "A Field Guide to Southern Mushrooms" by Smith and Weber, U of Mich Press, for 9.95. I have dealt with this firm for years, and they are trustworthy. No credit cards, no phone. Send a check, with $3.00 extra for shipping, or request the January 27 circular to see what else you want. You will end up on their mailing list forever. I once got a copy of Bigelow's 'Clitocybe Vol 1' from them for about 9 bucks. I have no commercial connection blah blah ted g gosfield@udcemail.udc.upenn.edu From owner-mycology@net.bio.net Fri Feb 03 22:00:00 1995 Newsgroups: bionet.mycology Path: biosci!hubcap.clemson.edu!biosci!bcm!cs.utexas.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!caen!zip.eecs.umich.edu!newsxfer.itd.umich.edu!nntp.cs.ubc.ca!news.UVic.CA!spruce.pfc.forestry.ca!PFC.Forestry.CA!RWINDER From: rwinder@PFC.Forestry.CA (Richard Winder) Subject: Re: mycorhizal fungi Message-ID: <1995Jan30.182138.1415@spruce.pfc.forestry.ca> Sender: news@spruce.pfc.forestry.ca Nntp-Posting-Host: pfc.pfc.forestry.ca Reply-To: rwinder@PFC.Forestry.CA Organization: Forestry Canada (Pacific Forestry Centre) References: <3g5juu$8rf@bud.peinet.pe.ca> Date: Mon, 30 Jan 1995 18:21:38 GMT Lines: 98 In article <3g5juu$8rf@bud.peinet.pe.ca>, stewart@bud.peinet.pe.ca (Paul Stewart) writes: >Fellow Myconuts: > Has anyone a contact for research or info regarding mycorhizal fungi? >Specifically, I'm looking for any attempts to culture them. My idea is >to try extracts of the companion roots or surrounding soil as additives >to agar and similar additives to the spawn mix (grain, wood chips, sawdust). > I have successfully grown mycelium cultures of Boletus edulis >(king bolete), chanterelles, and Suillus luteus (slippery Jack), all of >which are native to PEI, but not reliably plentiful enough to wild >harvest (not crazy about wild harvest anyway). I've heard from plenty of >people that I'm wasting my time, so I'd rather that not be my only reply >(Stamets' latest book seems to agree with me). You are not wasting your time. Certain boletes (including B. edulis) have been reportedly grown in axenic culture. Ditto for morels. It will take a bit of imagination and research to overcome the technical problems in a way that would be economically feasible, though. > Anyone with ideas to share please e-mail me or reply to this >group. I'll continue to be willing to share any of my own findings. I >have already identified some necessary trace elements from soil >containing Chanterelle mycelia. I just took samples from the mycelial >layer, the grass root zone, and below this, followed by similar samples a >few feet away. The mycelia seemed to accumulate manganese, boron, and >calcium in significant quantities. Any comments.....? Just some really crazy ideas, with apologies out there to those who may have already considered these things... For culture of mycorrhizal mushrooms in general, I wonder if some of the issues to contend with are: 1) Intactness/size of mycelium Let's face it- B. edulis can get pretty big. Many of these mushrooms probably need to achieve a certain mycelial size before they start pumping all that water and nutrients into a fruiting body (hence the use of deep dish agar cultures to produce Boletus spp.) 2) Water relations If the medium dries out before a fruiting body can get going, it will abort. Proper water flow could also be involved in wicking away staling compounds or inhibitory compounds such as phenolics. 3) Nutrient flux A clear source/sink flow of nutrients may need to be established. A disruption of this flux might also be necessary for fruiting. 4) Inhibitors Some seeds need ample water to leach away inhibitors linked with dormancy- it wouldn't be shocking to find them in fungi. At any rate, closed systems accumulate waste products, inhibitors, etc. much more readily than open ones. Hence the incorporation of orange slices in successful Armillaria spp. mushroom culture (I think that the ascorbic acid/Vitamin C/antioxidant helps the fungus deal with accumulated phenolic compounds and other inhibitors- but watch that pH!). 5) Templates The orange peels described above also provide a good hunk of cellulose which probably acts as a nutritious growth template. 6) Photoperiod/hormones The B. edulis in my front yard (no, you can't have my home address!) always fruit exactly on Canada day (July 1) with the Amanita muscaria 2 days behind. The involvement of photoperiod might be direct, or indirect (plant hormones, etc.). 7) Nutrient base Approximating more closely the amino acids and sugars present in a tree root which are being exported to the mycelium might be a big help. Pectins, suberin, and various cell wall constituents might help. Maybe you also need phosphorylated compounds, membrane compounds (glycolipids, DNA fragments, etc.), or triggers like c-AMP to indicate a "living" presence- who knows. 8) Microbes Chantrelles and truffles apparently have a good deal of bacteria incorporated into their sporocarps, which may indicate symbiosis with other microbes as well as plants. It might be that bacterial cell wall components could be incorporated into the medium to avoid the contamination problems posed by these symbionts, which are usually facultative saprobes. If the bacteria produce iron-abosorbing ionophores which the fungus can use, this might be another thing to look into. Various microbes (bacteria, yeasts) may not be true symbionts, but may aid in sporocarp formation by providing competitve stress or an environmental cue. Still ready to grow boletes commercially? -RSW RICHARD WINDER Title: Research Scientist Canadian Forest Service Phone: (604) 363-0773 Victoria, B.C. Internet: RWINDER@A1.PFC.Forestry.CA From owner-mycology@net.bio.net Fri Feb 03 22:00:00 1995 Path: biosci!GEMINI.OSCS.MONTANA.EDU!umbjc From: umbjc@GEMINI.OSCS.MONTANA.EDU (Jimmy Cutler) Newsgroups: bionet.mycology Subject: Medical Mycology Predoc Train. Date: 3 Feb 1995 10:56:32 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 68 Sender: daemon@net.bio.net Distribution: world Message-ID: Announcement: Establishment of a Medical Mycology Predoctoral Training Program (MMPTP) at Montana State University The number of fungal infections has increased dramatically over the past decade and is expected to continue to increase. People who have immunological deficiencies are especially at risk. The deficiencies may occur because of infectious diseases such as AIDS, or because of modern medical procedures such as kidney and bone marrow transplants, indwelling catheters, the use of corticosteroids, broad spectrum antibiotics and anti-tumor agents. The NIH recognizes the need for scientists trained to do research in medical mycology. To help address this need, we have been awarded a training grant from the National Institute of Allergy and Infectious Diseases to establish the MMPTP. MMPTP Faculty and Research Facilities: The intent of the MMPTP at MSU is to develop Ph.D. scientists who will pursue a research career in medical mycology. The faculty in the Program have varied interests and strengths. The result is a strong interdisciplinary approach to training the predoctoral students. The combined faculty utilize virtually all of the modern research facilities on campus which include: DNA/RNA laboratories; computer image analysis and phospho imaging systems; hybridoma laboratories; modern microscopic techniques; flow cytometry laboratories; and, mass and NMR spectrometers. MSU has a campus-wide computer network and is a major node for NSFnet which gives access to supercomputers. Ten research faculty from four departments at MSU serve as Ph.D. mentors. Most of the faculty have federally funded research programs and include: Clifford W. Bond, virologist; Diane L. Brawner, medical mycologist; Jim E. Cutler, medical mycologist and Dir. MMPTP; Gill G. Geesey, biofilms; Joan M. Henson, fungal molecular genetics; A.J. Jesaitis, neutrophil function and signal transduction; Mark A. Jutila, E- and L-selectins, inflammation and gamma/delta T-cells; Mark T. Quinn, neutrophil function and inflammation; Norman D. Reed, immunologist; and, Gary A. Strobel, fungal pathogens and endophytes, fungal natural products. MMPTP Training Opportunities: In addition to dissertation work, trainees have the following opportunities. Two to three months of clinical mycology laboratory training under the direction of Kevin C. Hazen, Ph.D. at the University of Virginia Health Sciences Center (UVAHSC). Dr. Hazen is Associate Director of Clinical Microbiology Laboratories and Director of Medical Mycology at UVAHSC. Yearly workshops in clincial mycology at MSU by Michael G. Rinaldi, Ph.D., Chief of Clinical Microbiology Laboratories at the Univ. Texas Health Sci. Center in San Antonio. The trainees will also take a science core which includes medical and general mycology courses at MSU. Trainee Expenses Provided by the MMPTP: Travel expenses will be provided for the trainees to participate in clinical mycology laboratory training at UVAHSC and to present their research findings each year at a national scientific meeting. Trainees will receive an annual stipend of $14,000 to defray living expenses and they will receive fee waivers fro graduate studies. Openings in the Program and Minimum Criteria for Acceptance: We currently have two trainees in the Program and we will have two more openings by Fall Semester (September) of 1995. Applications will be accepted until the openings are filled. Minimum criteria for admission are US citizenship, baccalaureate degree in science, GPA 3.0 in science courses and Graduate Record Exam combined verbal + quantitative score of 1150. Please note that women and minorities are especially encouraged to apply. Address for Inquiries and Further Information About Admission into the Program: Jim E. Cutler, Ph.D., Director MMPTP, Department of Microbiology, 109 Lewis Hall, Montana State University, Bozeman, MT 59717; FAX: 406-994-2373; INTERNET: umbjc@gemini.oscs.montana.edu. From owner-mycology@net.bio.net Fri Feb 03 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!news.sprintlink.net!uunet!hodes.com!netcomsv!gatekeeper.genencor.com!usenet From: gwhited@genencor.com Newsgroups: bionet.mycology Subject: Re: Morel Production Date: 4 Feb 1995 00:33:40 GMT Organization: Genencor International, Inc. - (415) 742-7500 Lines: 13 Message-ID: <3gui14$17h@gatekeeper.genencor.com> References: <3g5pne$qae@newsbf02.news.aol.com> NNTP-Posting-Host: 10.1.1.41 X-Newsreader: AIR News 3.X (SPRY, Inc.) > mycochef@aol.com (MYCOCHEF) writes: > try Paul Stammets of Fungi Perfecti in Olympia, > Wash. call information for the number (area code 206). > >>>> Full address and phone number: Paul Stamets Fungi Perfecti PO Box 7634 Olympia, WA 98507 (206) 426-9292 From owner-mycology@net.bio.net Fri Feb 03 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!vixen.cso.uiuc.edu!news.uoregon.edu!netnews.nwnet.net!selway.umt.edu!not-for-mail From: bact@selway.umt.edu (James E Gannon) Newsgroups: bionet.mycology Subject: Morphology in submerged culture Date: 4 Feb 1995 13:38:24 -0700 Organization: University of Montana Lines: 8 Message-ID: <3h0ok0$psn@selway.umt.edu> NNTP-Posting-Host: selway.umt.edu Summary: What Controls morphology in submerged culture? Keywords: Rhizopus, Morphology, Submerged Culture X-Newsreader: TIN [version 1.2 PL2] I have just begun working with submerged (liquid grown) cultures of Rhizopus. Form the same spore prep, some times I get discrete bead like morphology and others I get an "uncontrolled" filamentous mass? What specifically controls morphological type under submerged conditions? Newsgroups: bionet.mycology Subject: Pleurotus tuber-regium?? Date: Sat, 4 Feb 95 22:41:19 PST Organization: Teleport - Portland's Public Access (503) 220-1016 Lines: 29 Message-ID: <91022.rarnold@teleport.com> Reply-To: NNTP-Posting-Host: ip-pdx2-01.teleport.com X-Minuet-Version: Minuet1.0_Beta_17A X-POPMail-Charset: English I have read several reports that within Nigeria there exists a type of mushroom with an underground "tuber" or sclerotia that is edible. Scientific name is Pleurotus tuber-regium (=Lentinus tuber-regium). Common names are: ofuako (Mid-Western States) ohu (Western Nigeria) osu (East and Central parts) rumbagada (Hausa people of Northern part) ufetu (Asaba division of Mid-Western state) utun weromon (Ishan division of Mid-Western state) Literature states that this mushroom "occurs in both tropical and subtropical regions of the world. It...forms large spherical to ovoid, subterranean sclerotia which sometimes measures up to 30 cm." I am seeking a culture or sclerotia of this species. Any assistance in this matter would be greatly appreciated! Regards, Ralph D. Arnold fax: 1-503-423-7409 rarnold@teleport.com PO Box 889 Oregon City, OR 97045 USA voicemail: 1-503-656-4709 ------ Forwarded message ends here ------ From owner-mycology@net.bio.net Sun Feb 05 22:00:00 1995 Path: biosci!rutgers!gatech!howland.reston.ans.net!news.cac.psu.edu!news.pop.psu.edu!hudson.lm.com!tusk.lm.com!not-for-mail From: mspear@telerama.lm.com (mspear) Newsgroups: bionet.mycology Subject: Re: Morphology in submerged culture Date: 6 Feb 1995 11:15:13 -0500 Organization: Telerama Public Access Internet, Pittsburgh, PA Lines: 14 Message-ID: <3h5huh$gqj@tusk.lm.com> References: <3h0ok0$psn@selway.umt.edu> NNTP-Posting-Host: tusk.lm.com X-Newsreader: TIN [version 1.2 PL2] James E Gannon (bact@selway.umt.edu) wrote: > I have just begun working with submerged (liquid grown) cultures of > Rhizopus. Form the same spore prep, some times I get discrete bead like > morphology and others I get an "uncontrolled" filamentous mass? What > specifically controls morphological type under submerged conditions? > Followup-To: bionet.mycology Sender: news@athena.ulaval.ca Nntp-Posting-Host: ophiostoma.rsvs.ulaval.ca Content-Transfer-Encoding: 8bit Organization: Universite Laval Mime-Version: 1.0 Date: Mon, 6 Feb 1995 20:53:41 GMT Lines: 23 I was recently contacted by a graduate student in soil microbiology. She is working on Penicillium spp. she has isolated from the soil and she has found that all her cultures are contaminated with protozoa (probably amebas, she believes). She is looking for information on how to get rid of these little beasts (the protozoa of course, not the fungi !). If any of you has some helpful hints, please contact me and I will pass the information on to the student. Thanks for your help. Louis Bernier Forest Pathologist Faculte de foresterie et de geomatique Pavillon Charles-Eugene Marchand Universite Laval Sainte-Foy, Quebec, Canada G1K 7P4 Phone: 418-656-7655 FAX:418-656-7493 E-mail: Louis.bernier@sbf.ulaval.ca From owner-mycology@net.bio.net Sun Feb 05 22:00:00 1995 Path: biosci!LELAND.STANFORD.EDU!mattman From: mattman@LELAND.STANFORD.EDU (Matt Springer) Newsgroups: bionet.mycology Subject: Submerged culture Date: 6 Feb 1995 12:10:27 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <199502062010.MAA10779@elaine17.Stanford.EDU> NNTP-Posting-Host: net.bio.net Regarding morphology in submerged culture: I don't know about Rhizopus specifically, but in Neurospora, the concentration of either carbon or nitrogen source can make the difference between mycelial growth and short, stunted conidiophore formation (microcycle conidiation). Temperature shifts and light can also have an effect. A computer search of "microcycle" will give several of these references. -Matt Springer From owner-mycology@net.bio.net Mon Feb 06 22:00:00 1995 Path: biosci!rutgers!gatech!swrinde!cs.utexas.edu!uunet!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: mzieg1234@aol.com (MZieg1234) Newsgroups: bionet.mycology Subject: Companion culture? Date: 6 Feb 1995 20:45:42 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 33 Sender: root@newsbf02.news.aol.com Message-ID: <3h6jc6$mbh@newsbf02.news.aol.com> Reply-To: mzieg1234@aol.com (MZieg1234) NNTP-Posting-Host: newsbf02.mail.aol.com Mushroom cultivators are supposed to be secretive about successes but I would like to report something interesting. About a month ago I wrote about the possibility of combining two different mushroom types in the same bag and called it "companion culture." The idea was that the combination of an easy fruiter and difficult fruiter would be -well- fruitful. The presence of competition or transfer of chemical/physical factors might induce primordia in the recalcitrant species. Nobody responded and I have not seen anything in the literature but it seemed a reasonable hypothesis. I have had no luck with Grifola frondosa in the past- an occasional aborted primordia. In tests this month once again control bags showed no primordia but in 50%(believe me this is gigantic improvement) of the bags with two species have nicely developing Grifola fruitbodies! Curiously the second species is not producing fruitbodies? I am a scientist and know that many more tests need to be done and it all could be coincidence. I am currently repeating the tests and perhaps the results will be different. I am truly hesitant to even send this note because of my academic upbringing but wouldn't mind if somebody tried repeating this on a small scale if they have the time or inclination. It could be of some importance economically for growing both Grifola and even other species. Some of you full timers must have tried this? I can't quite bring myself to say what the other species are just yet but two worked and I have a feeling it is not too critical. <:) Mike Ziegler From owner-mycology@net.bio.net Mon Feb 06 22:00:00 1995 Path: biosci!lhc!biosci!rutgers!gatech!bloom-beacon.mit.edu!satisfied.elf.com!news2.near.net!news.delphi.com!usenet From: da5id Newsgroups: bionet.mycology Subject: Re: Black Bolete Date: Tue, 7 Feb 95 00:15:46 -0500 Organization: Delphi (info@delphi.com email, 800-695-4005 voice) Lines: 4 Message-ID: References: <3fv7gl$hue@usenetw1.news.prodigy.com> <3g5pvf$qbi@newsbf02.news.aol.com> NNTP-Posting-Host: bos1d.delphi.com X-To: MYCOCHEF just curious about whether there there are indeed any boletes growing in the northeast and if they are whether they are safe?... or...could you point us in the direction of somebody who would know? shanjks! From owner-mycology@net.bio.net Mon Feb 06 22:00:00 1995 Path: biosci!galaxy.ucr.edu!ratatosk.yggdrasil.com!news.duke.edu!godot.cc.duq.edu!hudson.lm.com!tusk.lm.com!not-for-mail From: mspear@telerama.lm.com (mspear) Newsgroups: bionet.mycology Subject: Re: Morphology in submerged culture Date: 6 Feb 1995 11:15:13 -0500 Organization: Telerama Public Access Internet, Pittsburgh, PA Lines: 14 Message-ID: <3h5huh$gqj@tusk.lm.com> References: <3h0ok0$psn@selway.umt.edu> NNTP-Posting-Host: tusk.lm.com X-Newsreader: TIN [version 1.2 PL2] James E Gannon (bact@selway.umt.edu) wrote: > I have just begun working with submerged (liquid grown) cultures of > Rhizopus. Form the same spore prep, some times I get discrete bead like > morphology and others I get an "uncontrolled" filamentous mass? What > specifically controls morphological type under submerged conditions? > Newsgroups: bionet.mycology Subject: Re: Black Bolete Date: Tue, 7 Feb 95 00:15:46 -0500 Organization: Delphi (info@delphi.com email, 800-695-4005 voice) Lines: 4 Message-ID: References: <3fv7gl$hue@usenetw1.news.prodigy.com> <3g5pvf$qbi@newsbf02.news.aol.com> NNTP-Posting-Host: bos1d.delphi.com X-To: MYCOCHEF just curious about whether there there are indeed any boletes growing in the northeast and if they are whether they are safe?... or...could you point us in the direction of somebody who would know? shanjks! From owner-mycology@net.bio.net Mon Feb 06 22:00:00 1995 Path: biosci!rutgers!gatech!swrinde!cs.utexas.edu!uunet!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: mycochef@aol.com (MYCOCHEF) Newsgroups: bionet.mycology Subject: Re: Honey Mushrooms are Tree killers? Date: 6 Feb 1995 22:43:23 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 12 Sender: root@newsbf02.news.aol.com Message-ID: <3h6q8r$o4q@newsbf02.news.aol.com> References: <3grrvk$ltt@onramp.arc.nasa.gov> Reply-To: mycochef@aol.com (MYCOCHEF) NNTP-Posting-Host: newsbf02.mail.aol.com Hi, go out and harvest those puppies, heat up a pan, separate the stems from the caps, strip the tough outer layer from the stems and slice the caps, add some good olive to the pan and saute those shrooms until a little crispy. now enjoy them while watching your trees die. look under the bark and you'll see the mycelium threads clinging to the tree and eating three squares a day. some folks get sick at that sight and some just get sick from the mushroom so be careful when serving large groups. as always, mycochef From owner-mycology@net.bio.net Mon Feb 06 22:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!swrinde!howland.reston.ans.net!news.cac.psu.edu!news.pop.psu.edu!hudson.lm.com!asia.lm.com!not-for-mail From: Richard W. Kerrigan Newsgroups: bionet.mycology Subject: Re: mycorhizal fungi Date: 7 Feb 1995 12:20:03 -0500 Organization: Telerama Public Access Internet, Pittsburgh, PA Lines: 22 Sender: sylvan@telerama.lm.com Message-ID: <3h8a43$9lp@asia.lm.com> References: <3g5juu$8rf@bud.peinet.pe.ca> <1995Jan30.182138.1415@spruce.pfc.forestry.ca> <3gloac$gvo@bud.peinet.pe.ca> NNTP-Posting-Host: asia.lm.com X-Newsreader: TIN [version 1.2 PL2] Paul Stewart (stewart@bud.peinet.pe.ca) wrote: : My sincere thanks to all who have responded so far to my inquiries into : mycorrhizal culture... [ETC.] I didn't see the following info posted, so: The research lab 'Station Champignons' at INRA-Bordeaux has pioneered this area very successfully for more than 15 years. Contact Dr. J.-M. Olivier at the station, BP 81, 33883 Villenave d'Ornon, FRANCE. They wrote the book on Truffle culture and have had other successes, e.g. Lactarius spp. You might also want to contact Dr. Makoto Ogawa of Japan, to learn about unsuccessful work on Matsutake. I don't have his address at hand but could look it up if you e-mail me directly at rwk@sylvanres.com. Good luck! -- Rick -- Richard W. Kerrigan, Research Department, Sylvan Spawn Laboratory, Inc. 1163 Winfield Rd., Cabot, PA 16032 USA e-mail: rwk@sylvanres.com phone: 412-352-1521 fax: 412-352-4062 "Success has a thousand parents; failure is an orphan" From owner-mycology@net.bio.net Mon Feb 06 22:00:00 1995 Path: biosci!adam.cc.sunysb.edu!news.sprintlink.net!cs.utexas.edu!uunet!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: mycochef@aol.com (MYCOCHEF) Newsgroups: bionet.mycology Subject: Re: Honey Mushrooms are Tree killers? Date: 6 Feb 1995 22:43:23 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 12 Sender: root@newsbf02.news.aol.com Message-ID: <3h6q8r$o4q@newsbf02.news.aol.com> References: <3grrvk$ltt@onramp.arc.nasa.gov> Reply-To: mycochef@aol.com (MYCOCHEF) NNTP-Posting-Host: newsbf02.mail.aol.com Hi, go out and harvest those puppies, heat up a pan, separate the stems from the caps, strip the tough outer layer from the stems and slice the caps, add some good olive to the pan and saute those shrooms until a little crispy. now enjoy them while watching your trees die. look under the bark and you'll see the mycelium threads clinging to the tree and eating three squares a day. some folks get sick at that sight and some just get sick from the mushroom so be careful when serving large groups. as always, mycochef From owner-mycology@net.bio.net Mon Feb 06 22:00:00 1995 Path: biosci!galaxy.ucr.edu!ratatosk.yggdrasil.com!news.duke.edu!godot.cc.duq.edu!newsfeed.pitt.edu!gatech!swrinde!cs.utexas.edu!uunet!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: mzieg1234@aol.com (MZieg1234) Newsgroups: bionet.mycology Subject: Companion culture? Date: 6 Feb 1995 20:45:42 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 33 Sender: root@newsbf02.news.aol.com Message-ID: <3h6jc6$mbh@newsbf02.news.aol.com> Reply-To: mzieg1234@aol.com (MZieg1234) NNTP-Posting-Host: newsbf02.mail.aol.com Mushroom cultivators are supposed to be secretive about successes but I would like to report something interesting. About a month ago I wrote about the possibility of combining two different mushroom types in the same bag and called it "companion culture." The idea was that the combination of an easy fruiter and difficult fruiter would be -well- fruitful. The presence of competition or transfer of chemical/physical factors might induce primordia in the recalcitrant species. Nobody responded and I have not seen anything in the literature but it seemed a reasonable hypothesis. I have had no luck with Grifola frondosa in the past- an occasional aborted primordia. In tests this month once again control bags showed no primordia but in 50%(believe me this is gigantic improvement) of the bags with two species have nicely developing Grifola fruitbodies! Curiously the second species is not producing fruitbodies? I am a scientist and know that many more tests need to be done and it all could be coincidence. I am currently repeating the tests and perhaps the results will be different. I am truly hesitant to even send this note because of my academic upbringing but wouldn't mind if somebody tried repeating this on a small scale if they have the time or inclination. It could be of some importance economically for growing both Grifola and even other species. Some of you full timers must have tried this? I can't quite bring myself to say what the other species are just yet but two worked and I have a feeling it is not too critical. <:) Mike Ziegler From owner-mycology@net.bio.net Mon Feb 06 22:00:00 1995 Path: biosci!agate!library.ucla.edu!psgrain!ticsa.com!ucthpx!itu1.sun.ac.za!k2011.jsmarais.sun.ac.za!pwc From: pwc@MATIES.SUN.AC.ZA (Dr PW Crous, Plantpatologie, Tel 4796) Newsgroups: bionet.mycology Subject: Hyphomycetes Date: Tue, 7 Feb 1995 16:47:49 GMT Organization: University of Stellenbosch, South Africa Lines: 13 Message-ID: NNTP-Posting-Host: 146.232.44.36 Does anyone know of a good paper illustrating/discussing the mode of conidiogenesis of the hyphomycete genera (conidial fungi) Gyrothrix Corda and Circinotrichum Nees. I think several papers suggest they have annellations near the apices of the conidiogenous cells. However, could these not be small denticles? (I ask this because of a weird strain I have isolated that appears to have the latter). On a similar note, does anyone know who erected (and where) the genus Vermiculariopsiella. I suspect its Castaneda & Kendrick (its also recent). Yours in anticipation Pedro Crous PWC@MATIES.SUN.AC.ZA From owner-mycology@net.bio.net Mon Feb 06 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!vixen.cso.uiuc.edu!prairienet.org!jdkirkla From: jdkirkla@prairienet.org (Justin D. Kirkland) Newsgroups: bionet.mycology Subject: Pluerotus Slide Show Date: 7 Feb 1995 15:31:54 GMT Organization: University of Illinois at Urbana Lines: 7 Message-ID: <3h83pa$98k@vixen.cso.uiuc.edu> NNTP-Posting-Host: firefly.prairienet.org In my previous posting I said I needed Pluerotus slides for a presentation. I have had a few responses, but I feel I need to clarify that I am looking for projector slides for a slide show on Oyster Mushroom production and not microscope slides. Any help would be appreciated. Thanks-Justin -- From owner-mycology@net.bio.net Mon Feb 06 22:00:00 1995 Path: biosci!rutgers!gatech!swiss.ans.net!prodigy.com!usenet From: VPGH51A@prodigy.com (Jim Berlstein) Newsgroups: bionet.mycology Subject: Re: Black Bolete Date: 7 Feb 1995 18:48:45 GMT Organization: Prodigy Services Company 1-800-PRODIGY Lines: 10 Distribution: world Message-ID: <3h8fad$l9m@usenetw1.news.prodigy.com> References: <3fv7gl$hue@usenetw1.news.prodigy.com> <3g5pvf$qbi@newsbf02.news.aol.com> NNTP-Posting-Host: inugap4.news.prodigy.com X-Newsreader: Version 1.2 Sure there boletes there. Some are good, some are bad. Like always, it is a species by species thing. I have found boletus edulis in the NE. (once) Also, I think Boletus bicolor was common and OK. There are lots of Tylopolus genus boletes, the bitter bolete, not good. July and August mostly, if I remember correctly From owner-mycology@net.bio.net Mon Feb 06 22:00:00 1995 Path: biosci!rutgers!gatech!newsfeed.pitt.edu!hudson.lm.com!news.pop.psu.edu!news.cac.psu.edu!psuvm!rea105 Organization: Penn State University Date: Tue, 7 Feb 1995 11:08:18 EST From: Klamachpin Achgeketum Message-ID: <95038.110818REA105@psuvm.psu.edu> Newsgroups: bionet.mycology Subject: United Kingdom and Deutschland Lines: 8 I am a University Scholar at Penn State University. I am currently workin g on a VCG analysis of V. fungicola. I am also in the initial stages of fillin g out the applications for the Marshall(UK) and the Fulbright(Deut.) scholarshi ps. Eventually I will need to decide at which institution I would like to cont inue my studies. It is my hope that I will be able to find a location where my skills may be of use. If you have any suggestions, or would like to here more information, please contact me by e-mail at REA105@psuvm.psu.edu. Many Thanks Ryan Argot From owner-mycology@net.bio.net Mon Feb 06 22:00:00 1995 Path: biosci!rutgers!gatech!swrinde!howland.reston.ans.net!torn!news.unb.ca!peinet.pe.ca!peinet.pe.ca!not-for-mail From: stewart@bud.peinet.pe.ca (Paul Stewart) Newsgroups: bionet.mycology Subject: cloning from storebought Date: 7 Feb 1995 15:30:33 -0400 Organization: PEINet, Charlottetown, Prince Edward Island, Canada Lines: 19 Sender: stewart@bud.peinet.pe.ca Distribution: world Message-ID: <3h8hop$2mc@bud.peinet.pe.ca> NNTP-Posting-Host: bud.peinet.pe.ca Keywords: shiitake, oyster Has anyone successfully established mycelia from grocery store shiitakes or oysters? Nobody on PEI seems to have mycelia in culture (if I'm wrong please contact me), and so far I'm too cheap to buy spawn commercially. Also, I'm trying to keep it simple so as to eventually sell kits for home kitchens using local waste material. I have managed to start yellow morel and chanterelle mycelia from spores, but don't expect to fruit them this century :-} . I have just put some oyster stipe inner tissue into malt yeast agar with poplar sawdust added...about 50 slants. So while I'm crossing fingers against contamination, please let me know if anybody has tried this. Seeking not to reinvent the wheel, just grease it...Paul ABIOGEN c/o Paul Stewart RR #2 Vernon Bridge Prince Edward Island CANADA C0A 2E0 (902) 651-3163 From owner-mycology@net.bio.net Mon Feb 06 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!cs.utexas.edu!uunet!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: stella3771@aol.com (Stella3771) Newsgroups: bionet.mycology Subject: Textbook reviewers wanted (paid) Date: 7 Feb 1995 16:52:02 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 11 Sender: root@newsbf02.news.aol.com Message-ID: <3h8q22$51s@newsbf02.news.aol.com> Reply-To: stella3771@aol.com (Stella3771) NNTP-Posting-Host: newsbf02.mail.aol.com I work for a science textbook publishing company (Benjamin/Cummings), and I am looking for college professors who can do some reviewing. This involves taking a look at what is in the current textbook and filling out a couple of forms about it. I am most interested in having professors who can review the evolutionary diversity chapters of an introductory biology book for nonmajors, although I have some openings in other units (plant physiology, animal physiology, and genetics.) I have been told by professors that the average chapter takes about two hours to review, and we pay $80.00/ chapter. If you are interested, please contact me by phone at (800) 950-2665 ext. 887, or by e-mail at TabindaK@bc.aw.com. *Please* don't respond to the e-mail address that this message came from. Thanks. From owner-mycology@net.bio.net Mon Feb 06 22:00:00 1995 Path: biosci!MANI.CBS.UMN.EDU!npv From: npv@MANI.CBS.UMN.EDU (Nora Plesofsky-Vig) Newsgroups: bionet.mycology Subject: calcium in fungi Date: 7 Feb 1995 12:54:08 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: <9502072052.AA01079@mani.cbs.umn.edu> Reply-To: nora@molbio.cbs.umn.edu NNTP-Posting-Host: net.bio.net I would appreciate some help in designing/assessing experiments to determine cytoplasmic calcium levels in Neurospora crassa or another fungus. Specific questions are: (1) Does the esterified fura-2 work in Neurospora for fluorescent measurements of calcium, or what are the drawbacks to its use in fungi? (2) Does the calcium ionophore ionomycin work in Neurospora, or is there a better alternative? Any handy literature references would also be appreciated. Nora Plesofsky-Vig From owner-mycology@net.bio.net Mon Feb 06 22:00:00 1995 Path: biosci!rutgers!gatech!newsxfer.itd.umich.edu!jobone!lynx.unm.edu!dns1.NMSU.Edu!bilbo!rpeter From: rpeter@nmsu.edu (Peter Herman x5495) Newsgroups: bionet.mycology Subject: Re: infection of fungi by protozoa Date: 7 Feb 1995 18:44:34 GMT Organization: New Mexico State University Lines: 43 Message-ID: <3h8f2i$s19@dns1.NMSU.Edu> References: NNTP-Posting-Host: bilbo.nmsu.edu In article Louis.bernier@sbf.ulaval.ca (Louis Bernier) writes: >I was recently contacted by a graduate student in soil microbiology. She is > >working on Penicillium spp. she has isolated from the soil and she has >found >that all her cultures are contaminated with protozoa (probably amebas, she >believes). She is looking for information on how to get rid of these little > >beasts (the protozoa of course, not the fungi !). If any of you has some >helpful hints, please contact me and I will pass the information on to the >student. > There are 2 good ways 1) Get the cultures sporulating. Suspend many spores in a surfactant (like 0.05% Tween 20). Do serial dilutions of the spores. Put a drop on drop of suspension on the plate and spread or run it around to single spore. Some of the colonies will be amoeba free 2) Make a metal or glass ring with feet (I use 0.5 cm slices of 20 mm pyrex test tubes with 3 mm glass beads welded on to the ring in 3 spots). Put sterile ring in sterile petri dish. Pour in agar so that the agar level is over the feet and about mid-ring. Inoculate contaminated culture in the ring when the agar is completely set and plates are dried. (you have to watch out for condensate). The fungus will grow down through the agar and out from under the ring. cut a bit of the growing front when it is 2-3 cm beyond the ring. You may need to repeat the process. Bacteria and protozoa stay inside the ring because they cannot penetrate the agar. I use this to clean up field isolates of watermolds all the time and it works like a charm. Thank yous to John R. Raper who thought of this technique eons ago! Hope this helps * Peter Herman, Dept. of Biology Phone: +1 505 646 4532 * * New Mexico State University Fax: +1 505 646 5665 * * Box 30001, Dept. 3AF * * Las Cruces, NM 88003 USA e-mail rpeter@nmsu.edu * From owner-mycology@net.bio.net Tue Feb 07 22:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!howland.reston.ans.net!torn!news.bc.net!newsserver.sfu.ca!moorelab.biol.sfu.ca!pinto From: Linda Pinto Newsgroups: bionet.mycology Subject: Blocking protein synthesis Date: 8 Feb 1995 01:02:12 GMT Organization: Simon Fraser University Lines: 6 Distribution: world Message-ID: <3h956l$dds@seymour.sfu.ca> NNTP-Posting-Host: @moorelab.biol.sfu.ca X-UserAgent: Version 1.1.3 X-XXMessage-ID: X-XXDate: Tue, 7 Feb 95 21:12:35 GMT I am working with a wild type (from soil) Penicillium strain which is resistant to cycloheximide (500 micrograms/ml). I would like to inhibit protein synthesis (in liquid culture) to see whether a certain enzyme is induced. Do you know of an appropriate antibiotic that works with filamentous fungi, and could you suggest an appropriate concentration? Thanks for any information! Linda From owner-mycology@net.bio.net Tue Feb 07 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!news.moneng.mei.com!uwm.edu!news.doit.wisc.edu!F181-170.net.wisc.edu!tjvolk From: tjvolk@facstaff.wisc.edu (Tom Volk) Newsgroups: bionet.mycology Subject: Re: Honey Mushrooms are Tree killers? Date: Wed, 8 Feb 1995 16:10:24 GMT Organization: U.S. Forest Service Lines: 50 Distribution: na Message-ID: References: <3grrvk$ltt@onramp.arc.nasa.gov> NNTP-Posting-Host: f181-170.net.wisc.edu Hi John. This is with regard to your Armillaria problem. In article <3grrvk$ltt@onramp.arc.nasa.gov> watson@pioneer.arc.nasa.gov (John S. Watson - FSC) writes: >Recently the brich in front of my parents house all died, >and around them were a bunch of small muchrooms that looked like >honey mushrooms, ( Armillariella [= Armillaria] mella ). >At a friends house near here I recently found much bigger >honey mushrooms all around 3 of his tree. >Acording to David Aura's book "Mushroom's Demystified", >honey mushrooms are parasite for which there is no cure, >and which eventually kills the host tree. >So does anyone know anymore about these guys? Is there >anything that can be done except sit back and enjoy the mushrooms? >So, are all these trees doomed? How long does it take to kill them? >Should trees killed by them be replaced in the same area, >and and should we wait a while to do so? As you probably know, what we used to call Armillaria mellea has been split up into 9-10 species of Armillaria in this country (aboutt 35-40 species worldwide). These are defined by matings as biological species. This is certaily not a purely academic exercise fromt he standpoint of patholoy. The different species vary widely in their pathogenicity and many are specif to hardwood or conifer. For example true Armilllaria mellea (Vahl:Fr) Kummer can be e sever pathogen of hardwoods, and doesn't occur much on conifer. Armillaria gallica Marxm. & Romagn (= A.bulbosa Velen.) is probably the most common species in the east, and is not very pathogenic. Evidence indicate it is probably just saprophytic. Armillaria ostoyae (Romagn.) Herink is normally a severe pathogen of conifers, but also occurs nearly half the time on hardwoods. I can't tell for sure from your post, but I think you;re in California. One study in California has addressed this problem. Jacobs, K.A. et al Identification of Armillaria species in California. Mycologia 86:113-116, 1994 Much more work needs to be done on pathogenicity of the various species in different parts of the country. What is clear, however, is that your birch tree is doomed. Once Armillaria takes hold, the rhizomorps and mycelial fans penetrate under the bark of the roots and the "butt " of the tree and destroy the cambial layer, everntually girdling the tree. As MycoChef pointed out, though, they are delicious. I have noted no real difference in flavor in any of the species, although you certainly want to cook them *very* well in any case. there are several reports of illness from eating undercooked Armillarias. This may be much more than you wanted to know, but it's kind of interesting to me-- I am in volved in writing a monograph of the North temperate species of Armillaria at present. Fell free to write if you have queestions. ---Tom Tom Volk, Center for Forest Mycology research, Forest Products Lab, Forest Service, Madison Wisconsin tjvolk@facstaff.wisc.edu From owner-mycology@net.bio.net Tue Feb 07 22:00:00 1995 Newsgroups: bionet.mycology Path: biosci!bloom-beacon.mit.edu!gatech!howland.reston.ans.net!vixen.cso.uiuc.edu!news.uoregon.edu!netnews.nwnet.net!news.pfc.forestry.ca!PFC.Forestry.CA!RWINDER From: rwinder@PFC.Forestry.CA (Richard Winder) Subject: Re: Companion culture? X-Nntp-Posting-Host: pfc.pfc.forestry.ca Message-ID: Sender: news@news.pfc.forestry.ca (0000-news(0000)) Reply-To: rwinder@PFC.Forestry.CA Organization: Forestry Canada (Pacific Forestry Centre) References: <3h6jc6$mbh@newsbf02.news.aol.com> Date: Tue, 7 Feb 1995 22:30:20 GMT Lines: 46 In article <3h6jc6$mbh@newsbf02.news.aol.com>, mzieg1234@aol.com (MZieg1234) writes: >Mushroom cultivators are supposed to be secretive about successes but I >would like to report something interesting. About a month ago I wrote about the >possibility of combining two different mushroom types in the same bag and >called it "companion culture." The idea was that the combination of an >easy fruiter and difficult fruiter would be -well- fruitful. The presence of >competition or transfer of chemical/physical factors might induce >primordia in the recalcitrant species. Nobody responded and I have not seen anything >in the literature but it seemed a reasonable hypothesis. I have had no luck >with Grifola frondosa in the past- an occasional aborted primordia. In tests >this month once again control bags showed no primordia but in 50%(believe me >this is gigantic improvement) of the bags with two species have nicely >developing Grifola fruitbodies! Curiously the second species is not producing >fruitbodies? I am a scientist and know that many more tests need to be >done and it all could be coincidence. I am currently repeating the tests and >perhaps the results will be different. I am truly hesitant to even send >this note because of my academic upbringing but wouldn't mind if somebody tried >repeating this on a small scale if they have the time or inclination. It >could be of some importance economically for growing both Grifola and even >other species. Some of you full timers must have tried this? I can't >quite bring myself to say what the other species are just yet but two worked and >I have a feeling it is not too critical. <:) Mike Ziegler I imagine Paul Stamets will have something to say about this. It is pretty well known that bacteria, when introduced at the proper time, can help promote fruiting body development of cultivated mushrooms. The fungal yeast Rhodotorula glutinus has been used to trigger basidiospore germination for various mushrooms. Bacteria have been implicated as symbionts with Chantrelles. There are people I know who hope, some day, to study the whole succession of fungi that attack tree stumps, and how they interact with each other. So your findings aren't out of line- there are probably all kinds of interactions going on. Many multiple-fungus cultures exhibit various degrees of antagonism- and competitive stress leading to the formation of sclerotia or fruiting bodies is one of the effects. In producing morel primordia on agar plates, I have noticed that contamination by the fungus Fusarium, if it occurs at such a point that the whole culture is not swamped with contamination, seems to enhance the development of larger sclerotia and primordia. I know you were hoping for mushrooms for both fungi, but I think its great you've got at least one of them going. -RSW RICHARD WINDER Title: Research Scientist Canadian Forest Service Phone: (604) 363-0773 Victoria, B.C. Internet: RWINDER@A1.PFC.Forestry.CA From owner-mycology@net.bio.net Tue Feb 07 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!news.sprintlink.net!news.clark.net!kcowing-ppp.clark.net!user From: kcowing@clark.net (Keith L. Cowing) Newsgroups: bionet.biology.grasses,bionet.genome.arabidopsis,bionet.mycology,bionet.photosynthesis,bionet.plants Subject: UPDATE: Call for Proposals: NASA Ground-Based and Small Payloads Research in Space Life Sciences Date: Wed, 08 Feb 1995 11:48:59 -0500 Organization: American Institute of Biological Sciences Lines: 174 Message-ID: NNTP-Posting-Host: kcowing-ppp.clark.net Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit Xref: biosci bionet.biology.grasses:206 bionet.genome.arabidopsis:2956 bionet.mycology:1505 bionet.photosynthesis:568 bionet.plants:5437 UPDATE: NASA NRA OLMSA 95-01 Now Available For Downloading The following NASA Research Announcement has been mailed (1/26/95) to a standard NASA distribution list. This NRA can be obtained by contacting the NASA officials at the email or postal addresses listed below. You can now download this entire NRA from the NASA OLMSA Life Sciences Gopher at NASA Headquarters (for Mac and PC) by selecting "Division Wide NRA" at either: lifegopher.hq.nasa.gov or via WWW at: gopher://lifegopher.hq.nasa.gov/ +++++++++++++ NASA OMB Approval No. 2700-0042 NRA 95 OLMSA-01 NASA Research Announcement Soliciting Proposals for Ground-Based and Small Payloads Research in Space Life Sciences Life and Biomedical Sciences and Applications Division Office of Life and Microgravity Sciences and Applications National Aeronautics and Space Administration Date NRA Issued: January 26, 1995 Letters of Intent Due: March 24, 1995 Proposals Due: April 21, 1995 This National Aeronautics and Space Administration (NASA) Research Announcement (NRA) solicits proposals to participate in Ground-Based and Small Payloads Research in Space Life Sciences. This is a broad-based announcement that solicits research proposals for all of the major programs of the Life and Biomedical Sciences and Applications Division. The specific programs that are included in this announcement are Space Biology, Space Physiology and Countermeasures, Environmental Health, Space Radiation Health, Space Human Factors, Advanced Life Support, Advanced Extravehicular Activity Systems, Advanced Technology Development and Data Analysis. Proposals submitted in response to this Announcement may be either for standard ground-based research investigations or for small payload flight experiments or for both. Proposal to develop experiments aiming at space station utilization are particularly encouraged. Subsequent announcements similar to and based upon this Announcement will be updated and issued annually and will be the primary means of obtaining research proposals form the scientific community for ground and small payloads research in the space life sciences. Although this NRA is broad-based, it is restricted to the science programs named above and described in detail in Appendix A. The potential proposer should read the program descriptions that are of interest with care and attempt to focus the proposal on the specific research emphases defined in this Announcement. Participation in the Ground-based and Small Payloads Research in Space Life Sciences is open to all individuals and all categories of domestic and foreign organizations, industry, educational institutions, other nonprofit organizations, NASA laboratories, and other U.S. Governmental agencies. Proposals which will enhance or complement the scientific return from research currently being supported by the National Institutes of Health, National Science Foundation, or other Government agencies are encouraged. In addition, proposals to advance technology and develop practical applications of technology are sought under this Announcement. Because of limitations of access to flight opportunities, it is expected that the majority of proposals approved in response to this Announcement would be for ground-based research. A letter of Intent to Propose is requested by March 24, 1995. Proposals may be submitted at any time up to April 21, 1995. Proposals will be evaluated for scientific/technical value, soundness, intrinsic scientific/technical value, relevance, implementation feasibility, and cost. A selection announcement will be made between August and October of 1995. Funding of selected proposals will begin sometime between October 1995 and September 1996. Proposals due: April 21, 1995 Letters of Intent Due: March 24, 1995 Number of copies Requested: 20 NASA Selecting official: Dr. Joan Vernikos Director, Life and Biomedical Sciences and Applications Division/ Code ULR NASA Headquarters Washington, DC 20564-0001 Obtain additional information from the appropriate Science Program manager, as indicated below, at the following address: UL/ Life and Biomedical Sciences and Applications Division NASA Headquarters Washington, DC 20546-0001 Telephone: 202-358-2530 Fax: 202-358-4168 Program: Program Manager/Email Space Biology: Dr. Mary Anne Frey MAFrey@smtpgmgw.olmsa.hq.nasa.gov Space Physiology and Countermeasures: Dr. Victor S. Schneider vschneider@smtpgmgw.olmsa.hq.nasa.gov Environmental Health: Dr. Victor S. Schneider vschneider@smtpgmgw.olmsa.hq.nasa.gov Space Radiation Health: Dr. Frank M. Sulzman fsulzman@smtpgmgw.olmsa.hq.nasa.gov Space Human Factors: Dr. Guy C. Fogleman GCFogleman@smtpgmgw.olmsa.hq.nasa.gov Advanced Life Support: Dr. Maurice M. Averner maverner@smtpgmgw.olmsa.hq.nasa.gov Advanced Extravehicular Activity Systems: Mr. Robert K. Callaway RCallaway@smtpgmgw.olmsa.hq.nasa.gov Advanced Technology Development: Mr. Gregory K. Schmidt gschmidt@smtpgmgw.olmsa.hq.nasa.gov Data Analysis: Dr. Ronald J. White RJWhite@smtpgmgw.olmsa.hq.nasa.gov Small Payloads Program: Mr. Marc Shepanek mshepanek@smtpgmgw.olmsa.hq.nasa.gov Proposals and Letters of Intent mailed through the U.S. Postal Service by express, first class, registered, or certified mail are to be sent to the following address: Ronald J. White, PhD. Life and Biomedical Sciences and Applications Division/ Code UL NASA Headquarters Washington, DC 20546-0001 Proposals and Letters of Intent hand delivered or sent by commercial delivery or courier services are to be delivered between the hours of 8 AM and 4:30 PM: Ronald J. White, PhD. Life and Biomedical Sciences and Applications Division/ Code UL National Aeronautics and Space Administration ATTN: Receiving and Inspection (Rear of Building) 300 E street, SW Washington, DC 20024-3210 The telephone number 202-488-2940 may be used when required for reference by delivery services. NASA cannot receive deliveries on Saturdays, Sundays, or federal Holidays. Special instructions apply to foreign (non-U.S.) proposals. From owner-mycology@net.bio.net Wed Feb 08 22:00:00 1995 Path: biosci!massey.ac.nz!S.Leangon From: S.Leangon@massey.ac.nz ("S. Leangon") Newsgroups: bionet.mycology Subject: How to break fungal mycelium Date: 8 Feb 1995 22:51:49 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <9502090650.AA02832@cc-server4.massey.ac.nz> NNTP-Posting-Host: net.bio.net I have 2 questions, any suggestions would be great appreciated. 1. Does anybody know the good procedure to break the fungal mycelium ? I am working with Aspergillus niger. I grow it on the surface of a semi- solid medium prepared from sweet potato root. The mycelium sheet of the old culture is rather hard to break in the porcelain mortar. 2. I am trying to assay ATP, AMP, NAD and NADH in this fungus. Does anyone ever experienced with this assays (especially in fungal mycelium). At the moment I follow the procedures from "Bergmeyer Methods of Enzymatic Analysis" but I am wondering if there are any modifications or cautions. Please contact me at : "S.Leangon@massey.ac.nz" From owner-mycology@net.bio.net Wed Feb 08 22:00:00 1995 Path: biosci!MOL.F.U-TOKYO.AC.JP!akiyama From: akiyama@MOL.F.U-TOKYO.AC.JP Newsgroups: bionet.mycology Subject: Expression of bigger protein than expected in E. coli Date: 8 Feb 1995 19:37:55 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <199502090337.MAA08211@mol.f.u-tokyo.ac.jp> NNTP-Posting-Host: net.bio.net Has anyone ever experienced the production of bigger polypeptide than expected while expressing protein in E. coli ? I expressed a plant enzyme in E. coli using pET system. I ligated my cDNA into the vector pET-3d just at the point of initiation codon, but E. coli horboring this plasmid produced two different polypeptide. They differ in size by about 1-2 kDa. Both react to the antibody against the original plant protein. First, I thought this was caused by protease (i.e. the bigger one is the native form). But I found that the "smaller" one has catalytic activity and the "bigger" doesn't. Now I assume the presence of additional unexpected amino acid sequence before the N-terminus or after the C-terminus. Has anyone encountered or heard of such situation ? If you have, please give me a good advise. (I am going to determine the N-terminal amino acid sequence of both polypeptide in the near future.) From owner-mycology@net.bio.net Wed Feb 08 22:00:00 1995 Newsgroups: bionet.mycology,bionet.parasitology,bionet.plants,bionet.biology.grasses Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!news.sprintlink.net!uunet!newsflash.concordia.ca!CC.UMontreal.CA!megasun!okellyc From: okellyc@megasun.BCH.UMontreal.CA (Charles J. O'Kelly) Subject: Taxonomic Research - PEET White Paper Available on WWW Message-ID: Followup-To: bionet.protista Keywords: taxonomy, systematics, NSF, funding, PEET Sender: okellyc@megasun (Charles J. O'Kelly) Organization: OGMP- Organellar Genome Megasequencing Project Date: Thu, 9 Feb 1995 00:25:31 GMT Lines: 79 Xref: biosci bionet.mycology:1506 bionet.parasitology:496 bionet.plants:5450 bionet.biology.grasses:207 ============================================================================== GUIDELINES FOR MONOGRAPHIC RESEARCH PROPOSALS TO THE PEET PROGRAM OF NSF AVAILABLE ON WORLD WIDE WEB ====================================================================== The National Science Foundation has released a White Paper on the PEET (Partnerships for Enhancement of Expertise in Taxonomy) program. The report is titled: TAXONOMIC EXPERTS, SYSTEMATIC MONOGRAPHS REPORT OF A PANEL-WORKSHOP, 29-30 NOVEMBER 1994 ON PRACTICES AND DIRECTIONS IN MONOGRAPHIC RESEARCH and contains a number of specific comments and recommendations for both writers and reviewers of PEET proposals. The document may be found at the following URL address: http://megasun.bch.umontreal.ca/isep/peet.html and it may also be accessed from the ISEP home page http://megasun.bch.umontreal.ca/isep/isep.html by selecting the line "PEET Report". The report is also available by email from okellyc@bch.umontreal.ca (Charles J. O'Kelly), if you don't have access to the World Wide Web. If you are not familiar with the PEET initiative, the full description may be found in document NSF 94-109 from the Directorate of Biological Sciences, Division of Environmental Biology, (US) National Science Foundation. "The National Science Foundation (NSF), in partnership with academic institutions, botanical gardens, freshwater and marine institutes, and natural history museums, seeks to enhance and stimulate taxonomic research ... NSF announces a special competition ... to support competitively reviewed research projects that target groups of poorly known organisms. ... Three major elements are required of a project submitted ...: 1. Monographic Research; 2. Training; and 3. Computer Infrastructure. The deadline for receipt of proposals is 1 March 1995. Further information: Division of Environmental Biology (PEET) National Science Foundation 4201 Wilson Boulevard, suite 635 Arlington, VA 22230 USA tel: (1) 703 306 1481 fax: (1) 703 306 0367 email: sysrev@nsf.gov To get the whole document by electronic mail, mail to: stisserve@nsf.gov Leave the subject line blank. Send the following message: get nsf94109 ========================== charley o'kelly mad protistologist okellyc@bch.umontreal.ca From owner-mycology@net.bio.net Wed Feb 08 22:00:00 1995 Path: biosci!CSUVAX1.MURDOCH.EDU.AU!wylie From: wylie@CSUVAX1.MURDOCH.EDU.AU (Steve Wylie) Newsgroups: bionet.mycology Subject: PAL induced by R. solani Date: 8 Feb 1995 21:30:54 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I have tested several methods to analyse PAL activity from whole plants(tissue cultured tobacco) whose defense mechanisms have been induced by the eliciter Rhizoctonia solani.I have observed different PAL activity in the plants when inoculated with different eliciter concentrations. However, I have been unable to quantify PAL activity by any of the means tested. The methods I have tried are those of Bhattacharyya & Ward(1988) and Hillis & Ishikura(1970). Any suggestions would be most welcome. Steve Wylie wylie@murdoch.edu.au From owner-mycology@net.bio.net Wed Feb 08 22:00:00 1995 Path: biosci!rutgers!gatech!swiss.ans.net!prodigy.com!usenet From: VPGH51A@prodigy.com (Jim Berlstein) Newsgroups: bionet.mycology Subject: Re: GUARANTEED CREDIT REPAIR BY LAW FIRM Date: 9 Feb 1995 15:14:33 GMT Organization: Prodigy Services Company 1-800-PRODIGY Lines: 8 Distribution: world Message-ID: <3hdbgp$q7i@usenetw1.news.prodigy.com> NNTP-Posting-Host: inugap4.news.prodigy.com X-Newsreader: Version 1.2 How dare you post this crap in the bionet.mycology section? You should be ashamed of yourselves. You will only succeed in convincing people that you are slime. Credit repair? We don't need no stinkin credit repair. From owner-mycology@net.bio.net Wed Feb 08 22:00:00 1995 Path: biosci!bcm!usenet From: gsmay@bcm.tmc.edu (Gregory May) Newsgroups: bionet.mycology Subject: Abstract Date: 9 Feb 1995 14:54:21 GMT Organization: Baylor College of Medicine Lines: 41 Message-ID: <3hdaat$cao@gazette.bcm.tmc.edu> NNTP-Posting-Host: ai.cellb.bcm.tmc.edu X-Newsreader: WinVN 0.92.6+ The following is an abstract of a paer that is in press. It will appear in the Journal of Cell Biology Volume 128, No. 4, February 1995. myoA of Aspergillus nidulans encodes an essential myosin I required for secretion and polarized growth. Carol A. McGoldrick, Carol Gruver and Gregory S. May ABSTRACT We have identified and cloned a novel essential myosin I in Aspergillus nidulans, called myoA. The 1249 amino acid predicted polypeptide encoded by myoA is most similar to the amoeboid myosins I. Using affinity purified antibodies against the unique myosin I carboxyl terminus, we have determined that MYOA is enriched at growing hyphal tips. Disruption of myoA, by homologous recombination, resulted in a diploid strain heterozygous for the myoA gene disruption. We can recover haploids with an intact myoA gene from these strains but never haploids that are myoA disrupted. These data indicate that myoA encodes an essential myosin I and this has allowed us to use a unique approach to studying myosin I function. We have developed conditionally null myoA strains in which myoA expression is regulated by the alcA alcohol dehydrogenase promoter. A conditionally lethal strain germinated on inducing medium grows as wild type, displaying polarized growth by apical extension. However, growth of the same myoA mutant strain on repressing medium results in enlarged cells incapable of hyphal extension and these cells eventually die. Under repressing conditions, this strain also displays reduced levels of secreted acid phosphatase. The mutant phenotype indicates that myoA plays a critical role in polarized growth and secretion. =============================================== Gregory S. May Department of Cell Biology Baylor College of Medicine One Baylor Plaza Houston, TX 77030 Tel. 713-798-4756 FAX. 713-798-7799 E-mail gsmay@bcm.tmc.edu =============================================== From owner-mycology@net.bio.net Wed Feb 08 22:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!swrinde!howland.reston.ans.net!news.cac.psu.edu!news.tc.cornell.edu!caen!msunews!harbinger.cc.monash.edu.au!news.cs.su.oz.au!metro!news.une.edu.au!usenet From: Michael Adamson Newsgroups: bionet.mycology Subject: Looking for pathogenic Pleurotus Date: 9 Feb 1995 00:21:50 GMT Organization: UNE Lines: 3 Distribution: world Message-ID: <3hbn6u$kdm@grivel.une.edu.au> NNTP-Posting-Host: biol1-01.une.edu.au X-UserAgent: Nuntius v1.0 I recently heard from a FOAF that someone had isolated a Pleurotus from rotting tubers of Cyperus sp. Anyone have any idea what species of Pleurotus might be responsible? From owner-mycology@net.bio.net Wed Feb 08 22:00:00 1995 Path: biosci!rutgers!gatech!swrinde!howland.reston.ans.net!vixen.cso.uiuc.edu!news.uoregon.edu!gaia.ucs.orst.edu!news.uidaho.edu!xslip07.csrv.uidaho.edu!user From: bmiller@uidaho.edu (Bruce L. Miller) Newsgroups: bionet.mycology Subject: Faculty Position Available Date: Fri, 03 Feb 1995 10:14:00 -0800 Organization: University of Idaho Lines: 26 Message-ID: NNTP-Posting-Host: xslip07.csrv.uidaho.edu Mime-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit Assistant Professor of Molecular Biology and Biochemistry: The University of Idaho is seeking to fill a tenure track position for an assistant professor. We are interested in candidates capable of establishing a nationally competitive research program to study the molecular biology of plants or fungi in one of two areas: 1) molecular mechanisms of development or 2) responses to abiotic and biotic stimuli. The research program should complement existing departmental programs in plant molecular biology and biochemistry, microbial developmental genetics, medical/pathogenic microbiology and environmental microbiology. The successful candidate must have a strong commitment to teaching excellence and will be responsible for a one semester undergraduate lecture/laboratory course in basic biochemistry to be taught each year and a graduate level course in his/her area of expertise in alternate years. Applicants should have a Ph.D. in Molecular Biology, Biochemistry or related field and postdoctoral research experience using contemporary molecular techniques. Send CV, statement of research interests and three letters of reference by March 24, 1995 to Bruce L. Miller, Search Committee Chair, Department of Microbiology, Molecular Biology and Biochemistry, Life Sciences Bldg., University of Idaho, Moscow, ID 83844. FAX (208) 885-6518. AA/EOE -- Bruce L. Miller Dept. of Microbiology, Molecular Biology & Biochemistry University of Idaho Moscow, ID 83843 From owner-mycology@net.bio.net Wed Feb 08 22:00:00 1995 Path: biosci!CYBERGATE.COM!jolivas From: jolivas@CYBERGATE.COM (John L. Olivas) Newsgroups: bionet.mycology Subject: Re: Taxus Date: 9 Feb 1995 14:07:18 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: <9502092202.AA03889@CyberGate.COM> NNTP-Posting-Host: net.bio.net I have information on Taxus and Arctostaphylos for the people that were interested. However, the address I have is incorect. Email me back with a deliverable address. John Olivas jolivas@cybergate.com From owner-mycology@net.bio.net Wed Feb 08 22:00:00 1995 Path: biosci!NEXTLAB.CALSTATELA.EDU!spencal From: spencal@NEXTLAB.CALSTATELA.EDU (Steve Pencall) Newsgroups: bionet.mycology Subject: Comment on Forest Service Collecting policy Date: 9 Feb 1995 12:36:24 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 57 Sender: daemon@net.bio.net Distribution: world Message-ID: <9502092035.AA27863@nextlab.calstatela.edu> NNTP-Posting-Host: net.bio.net TO: ALL AMATEUR AND PROFESSIONAL MYCOLOGISTS SUBJECT: Forest Service Comment Period to close February 15! The COMMENT PERIOD on the U.S. Forest Service's Draft Strategy For Special Forest Product Resources closes on February 15. The Draft Strategic Plan on Special Forest Product Resources is expected to become a guideline for local Forest policies on the collection of mushrooms and a host of other non-timber forest products. Therefore, it is vitally important for all mushroom collectors who collect in National Forests, which is most of us, to have their say as to how mushroom picking will be regulated. If we don't speak up on our own behalf, we risk having the policy being determined by a few anti-picking extremists. Unfortunately, throughout the entire development of this Plan, the Forest Service has made little or no effort to involve the public in formulation of the Plan, including the tens of thousands nationwide who gather and use "Special Forest Products". I am unaware of any of the approximately 75 mycological societies having been contacted about the Plan, nor any individual members of those societies, nor any of the publications which address this audience. Nor am I aware of any other affected groups that have been contacted either. The Plan has not even been published in The Federal Register! The draft Special Forest Products Plan was finally published on October 27 with an allotted comment period of only 27 days!--far too little time for most of the affected groups to inform most of their members of the opportunity to comment on the policy. It is difficult to escape the conclusion that the reason for the actions that I have related is to suppress public input to the Plan. These actions are in direct contradiction of the avowed goals of Forest Service "reinvention". It seems unlikely that anyone in the mycological community would be aware of the Plan even today, were it not for a chance remark made to a member of the Mycological Society of San Francisco by a staffer in the Regional Office of the Forest Service in San Francisco. I wonder if the Forest Service will attempt to discover and punish the person who "leaked" word of this Plan? At the time the Plan was first published in October, the public comment period was a mere 27 days! Now due to protests by this writer and others, the comment deadline has been extended an additional 12 weeks, to February 15, 1995. You are urged to write or fax the Forest Service and tell them that the Special Forest Products Plan needs additional changes to fairly address the concerns of amateur mushroom collectors. For a list of specific points to include in your letter, please contact this writer. Mr. Mike Higgs USDA Forest Service, CF 4SE AUD P.O. Box 96090 Washington, DC 20090 Phone: (202) 273-4754 Fax: (202) 205-0975 Office hours are 8 am to 5 pm Eastern Time Please state that you are commenting on the Draft Strategic Plan for Managing Special Forest Product Resources. You should also request a copy of the Plan. From owner-mycology@net.bio.net Thu Feb 09 22:00:00 1995 Path: biosci!ESSEX.HSC.COLORADO.EDU!claudes From: claudes@ESSEX.HSC.COLORADO.EDU (Claude Selitrennikoff) Newsgroups: bionet.mycology Subject: Re: GUARANTEED CREDIT REPAIR BY LAW FIRM Date: 9 Feb 1995 16:06:14 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <3hct7g$t1u@panix.com> NNTP-Posting-Host: net.bio.net Dear Credit Advocates: I object strongly to your advertising on this network. This is not appropriate and I find this behavior highly objectionable. Claude P. Selitrennikoff, Ph.D. Professor From owner-mycology@net.bio.net Thu Feb 09 22:00:00 1995 Path: biosci!aol.com!MZieg1234 From: MZieg1234@aol.com Newsgroups: bionet.mycology Subject: More on Companion Cultures Date: 9 Feb 1995 22:07:43 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 47 Sender: daemon@net.bio.net Distribution: world Message-ID: <950209231038_17556005@aol.com> NNTP-Posting-Host: net.bio.net Ralph Arnold suggested I expand on my note of the other day concerning primordia formation in Grifola frondosa(GF)in "companion" cultures. The substrate conditions were similar to those I have used for growth of many other wood-inhabiting fungi. Grifola was expanded from MEA agar to winter wheat to sawdust fruiting substrate. 500ml volume of wheat spawn was used per 5lb bag. Substrate is 94% sawdust (8/2 oak sawdust/maple chips), 5% organic wheat bran, 1% gypsum. Moisture was to the point of a few drops when squeezed hard. Each bag also had about 1 Tbs. blackstrap molasses. Autoclave.... Incubation is in the dark at 72F. Experimental bags had additional volume of companion fungus added at the beginning of incubation. Bags were sealed and agitated to give through-spawn conditions. One species was added at equivolume wheat spawn. The other was added as50mls liquid innoculum. Incubation was for 40 days, then star shaped holes(2)were made at the top corners of the bag and all gasses expelled and the top of the bag tied down per suggestion of Annette at Northwest Myc Cons. Temperature was dropped to 60 degrees at 85-95% humidity. I have heard that Asian cultivators give GF a real cold shock-40's for 24-48 hours but I haven't tried this . Both positive species interact very little with GF, that is when incubated together the mycelium grow through one another without forming interaction zones as do some species e.g. trichoderma. One species that gave negative results was sulfur shelf. Interaction zones did form here and hard raised orange plateaus resulted( I had Fall conditions and might try Summer instead here to mimic sulfur shelf requirement s next time)! Both species which gave positive results were cool weather fruiters-one Spring and one Fall. As I noted before both sets of bags gave primordia of GF only even though control bags grew and fruited nicely. I have been trying dual Morel cultures(I know it doesn't make any sense considering the published lifecycle but what the heck, this is only a weird hobby) but find that it overgrows so quickly that companion mycelium get esta blished only minimally. I will be adjusting innoculum accordingly in future tries. As I said before these results are PRELIMINARY and may not pan out, but given my singular failings with GF in the past they certainly took me by surprise. Since there is nothing patentable here (anybody is free to throw two mushrooms in a bag thank you) I feel OK sharing this much and hope that anyone can grow any mushroom he or she pleases for both pleasure and profit and anything I can do to help I will. I would be happy to receive any comments on the above. Happy growing to all who try. Mike Ziegler from SNOWY Vermont. From owner-mycology@net.bio.net Thu Feb 09 22:00:00 1995 Path: biosci!rutgers!gatech!swrinde!cs.utexas.edu!news.sprintlink.net!aimnet1.aimnet.com!aimnet.aimnet.com!not-for-mail From: cina@aimnet.com (Chinese Information and Networking Association) Newsgroups: bionet.immunology,bionet.biology,tropical,bionet.mycology Subject: SOS, help save the Chinese girl's life Date: 9 Feb 1995 21:36:25 -0800 Organization: Aimnet Information Services, Cupertino, CA Lines: 70 Distribution: world Message-ID: <3heu0p$s7u@aimnet.aimnet.com> NNTP-Posting-Host: aimnet.aimnet.com Xref: biosci bionet.immunology:3109 bionet.mycology:1519 Call For Help! Non-infection Necrosis? Infectious necrosis? or Localized menifestation of an unknown systemic disease? --- A young Chinese girl is slowly being eaten away by a strange disease. A young Chinese girl suffering from a mysterious infection that causes progressive necrosis of her right forearm and left hand is arousing great concern in the whole country. After fighting the disease for nearly four months without any success, doctors in Beijing are appealing for emergency help from colleagues arround the world. The thirteen year old girl, Yang Xiaoxia comes from a poor farm family in Shangdong province in east China. The girl has been tortured by the mysterious disease for 8 months. The disease first began as a black spot under a finger nail of her right hand suspected by a local clinic as a stab wound caused by small stick or barb. A local doctor tried to get something out of the black spot, but thing got worse. She began to suffer from paronychiais and the infection soon spread to the whole right forearm and the mid-finger on her left hand. After treatments in five different hospitals in provincial capital Jinan had all failed, the girls parents took her to Beijing. She has hospitalized in a Beijing military hospital since early November, but the disease continued to progress despite all out efforts by the city's most elite doctors. The doctors have to amputate one third of her right forearm and three fingers of her left hand. Although the spreed of the infection seems a little bit slower, still there was no sign the infection will stop. What puzzled the whole medical community is that from the appearance of the affected surfaces it looks like an infection, but the infection have failed to response to any exsiting antibiotics and hyperbaric oxgen treatment. Bacterior cultures have always been negtive. Officals of the city public health bureau have invited experts to probe into the mystirous disease and welcomes any suggestions from doctors around the world. We will continue to update information on Yang Xiaoxia's condition. If you have any suggestions, please send e-mail to the following address: Huangxd@BEPC2.IHEP.AC.CN Dr. Xiangdong Huang Institute of Medical Information, 27 Taiping Road, Beijing 100039, China. Tel:86-1-8215603 Fax:86-1-8213044 From owner-mycology@net.bio.net Thu Feb 09 22:00:00 1995 Path: biosci!aol.com!MZieg1234 From: MZieg1234@aol.com Newsgroups: bionet.mycology Subject: re:cloning from storebought Date: 9 Feb 1995 20:37:26 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: <950209230443_17555242@aol.com> NNTP-Posting-Host: net.bio.net Paul- It certainly is possible to clone from storebought! My first Shitake cultures were from the grocery and fruited just fine on sawdust substrate. Shitakes keep well so I think your chances are better with them than Oysters. The Oysters I find in the stores are usually slimy and ugh. Although obtaining cultures this way is fine you may determine later that you want to know more about the fruiting characteristics prior to innoculating lots of logs. Multiyear or commercial efforts are worth the few dollars it would cost for spawn and the companies out there do all the research for you for very little money considering. I suppose legally the storebought can be expanded for sale but you might need to check with a lawyer on that. If you are just fooling around with a few bags of sawdust-go for it!! Mike Ziegler From owner-mycology@net.bio.net Thu Feb 09 22:00:00 1995 Path: biosci!CYBERGATE.COM!jolivas From: jolivas@CYBERGATE.COM (John L. Olivas) Newsgroups: bionet.mycology Subject: Alginate Date: 10 Feb 1995 10:41:26 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 22 Sender: daemon@net.bio.net Distribution: world Message-ID: <9502101836.AA13845@CyberGate.COM> Fellow Netters, I am looking for a copy of the following article: AU: Strullu,-D.G.; Plenchette,-C. TI: The entrapment of Glomus sp. in alginate beads and their use in root inoculum. SO: Mycol-Res. Cambridge : Cambridge University Press. Oct 1991. v. 95 (pt.10) p. 1194-1196 CN: DNAL QK600.M82 Any assistance or advise would be greatly appriciated. Regards, John Olivas BioScientific, Inc. 3574 W. Escalon Ave. Fresno, CA. 93711 Ph. 209-269-1152 Fax. 209-439-8639 Email jolivas@cybergate.com From owner-mycology@net.bio.net Fri Feb 10 22:00:00 1995 Path: biosci!rutgers!gatech!swrinde!cs.utexas.edu!news.sprintlink.net!uunet!in1.uu.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: brianmc@aol.com (BrianMc) Newsgroups: bionet.mycology Subject: Re: Honey Mushrooms are Tree killers? Date: 11 Feb 1995 00:03:49 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 15 Sender: root@newsbf02.news.aol.com Message-ID: <3hhgfl$qaf@newsbf02.news.aol.com> References: <3grrvk$ltt@onramp.arc.nasa.gov> Reply-To: brianmc@aol.com (BrianMc) NNTP-Posting-Host: newsbf02.mail.aol.com Tom Volk is the current "Expert of Note" on the topic of Armillaria. If he says it's so, I'm certainly not about to disagree. Armillaria has been something of a running topic of late, and I've picked up on a few threads here for inclusion in my e-mail newsletter, MycoInfo. If the participants don't mind, I'd like to cross-post this discussion as well. Reports from 1992 of "Humoungous Fungus" from upper Michigan, and SW Washington state, were both species of Armillaria. It definitely one of the more ubiquitous fleshy fungi. ---------------------------------------------------------- | Brian McNett e-mail: | Editor: MycoInfo Submissions: | Promoting Mycology in the Online Community From owner-mycology@net.bio.net Sat Feb 11 22:00:00 1995 Path: biosci!adam.cc.sunysb.edu!news.sprintlink.net!howland.reston.ans.net!swiss.ans.net!prodigy.com!usenet From: VPGH51A@prodigy.com (Jim Berlstein) Newsgroups: bionet.mycology Subject: Early Seattle mushrooms Date: 11 Feb 1995 10:09:45 GMT Organization: Prodigy Services Company 1-800-PRODIGY Lines: 17 Distribution: world Message-ID: <3hi2d9$uj2@usenetw1.news.prodigy.com> NNTP-Posting-Host: inugap4.news.prodigy.com X-Newsreader: Version 1.2 While strolling through some local woods and ball fields with my fungus hunting budies (Great Danes, of course) I spotted some spring entalomas (in grass) and and some Amianita Pantherinas (in woods). Apparently winter is coming on sunday, but I was wondering if others have seen any extremely early fruitings. Also, does anyone think that this weird weather will bring on morel season early as well? Anyone find any verpas yet? Some nice morels appeared last year were the city did some digging by the "mailbox rack" in front of my neihbors house. As he has graded his trailer parking area only twenty feet away from there, I'm expecting BIG things this year! Waiting for spring in Seattle - JIM BERLSTEIN VPGH51A@prodigy.com From owner-mycology@net.bio.net Sat Feb 11 22:00:00 1995 Path: biosci!newshost.lanl.gov!news.ttu.edu!seas.smu.edu!convex!insosf1.infonet.net!solaris.cc.vt.edu!news.bluesky.net!udel!gatech!swrinde!howland.reston.ans.net!nntp.crl.com!crl.crl.com!not-for-mail From: kasplash@crl.com (Bob Galvan) Newsgroups: bionet.mycology Subject: Re: Early Seattle mushrooms Date: 11 Feb 1995 09:29:14 -0800 Organization: CRL Dialup Internet Access (415) 705-6060 [login: guest] Lines: 5 Message-ID: <3his5a$hrl@crl.crl.com> References: <3hi2d9$uj2@usenetw1.news.prodigy.com> NNTP-Posting-Host: crl.com Here in Marin county, California Amanita pantherina has been common as cockroaches all January, and fading fast now as Amanita velosa pops up by every Coast Live Oak. Lots of A. rubescens too. Poor year for chanterelles here, though there have been good reports 50-100 miles south. From owner-mycology@net.bio.net Sat Feb 11 22:00:00 1995 Path: biosci!rutgers!uwm.edu!caen!newsxfer.itd.umich.edu!jobone!lynx.unm.edu!dns1.NMSU.Edu!bilbo!rpeter From: rpeter@nmsu.edu (Peter Herman x5495) Newsgroups: bionet.mycology Subject: Re: calcium in fungi Date: 10 Feb 1995 20:07:45 GMT Organization: New Mexico State University Lines: 20 Distribution: world Message-ID: <3hgh2h$avl@dns1.NMSU.Edu> References: <9502072052.AA01079@mani.cbs.umn.edu> NNTP-Posting-Host: bilbo.nmsu.edu In article <9502072052.AA01079@mani.cbs.umn.edu> nora@molbio.cbs.umn.edu writes: >I would appreciate some help in designing/assessing experiments to >determine cytoplasmic calcium levels in Neurospora crassa or another >fungus. Specific questions are: > > (1) Does the esterified fura-2 work in Neurospora for >fluorescent measurements of calcium, or what are the drawbacks to its >use in fungi? > > (2) Does the calcium ionophore ionomycin work in Neurospora, I have had some luck in using it in the watermold Achlya. My problem had to do with keeping the mycelium in place when we exposed it to the growth regulator we were testing. Even thin agar didn't allow quick diffusion of the regulator and gave messy backgrounds. * Peter Herman, Dept. of Biology Phone: +1 505 646 4532 * * New Mexico State University Fax: +1 505 646 5665 * * Box 30001, Dept. 3AF * * Las Cruces, NM 88003 USA e-mail rpeter@nmsu.edu * From owner-mycology@net.bio.net Sat Feb 11 22:00:00 1995 Path: biosci!bcm!news.msfc.nasa.gov!cs.utk.edu!gatech!howland.reston.ans.net!news.sprintlink.net!uunet!in1.uu.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: wharrison@aol.com (WHARRISON) Newsgroups: bionet.mycology Subject: Re: Growing mushrooms on Internet Date: 11 Feb 1995 17:46:12 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 20 Sender: root@newsbf02.news.aol.com Message-ID: <3hjenk$6tu@newsbf02.news.aol.com> References: Reply-To: wharrison@aol.com (WHARRISON) NNTP-Posting-Host: newsbf02.mail.aol.com You can also subscribe to the newsletter of the Colorado Mycological Society through the internet. It's free, and chuck full of interesting articles, if I do say so myself. I'm the editor. _____________________ | ~ ^ | | ^ /_\ ^ ~ | | /_\ / \ / \ | | /\ / \ ^ \ __\/\| |/ / \/|\ \ \ | | / ^ /|\ ^ \| | / /|\ ^ | /|\ ^ | |/ /|\ /|\ /|\ /|\| | | | | | | |_____________________| Wayne Harrison Pine, Colorado elevation 8,500 ft. (way up there) From owner-mycology@net.bio.net Sat Feb 11 22:00:00 1995 Path: biosci!adam.cc.sunysb.edu!news.sprintlink.net!howland.reston.ans.net!vixen.cso.uiuc.edu!prairienet.org!peburnsx From: peburnsx@prairienet.org (Pamela E. Burns-Balogh) Newsgroups: bionet.mycology Subject: Natural History BBS Date: 11 Feb 1995 13:06:33 GMT Organization: University of Illinois at Urbana Lines: 11 Message-ID: <3hicop$phd@vixen.cso.uiuc.edu> NNTP-Posting-Host: firefly.prairienet.org Our mycology catalog is now available for downloading off our direct dial BBS, modem number is 1-217-355-4532, set your your screen to ANSI, download protocol to ZMODEM, takes about 20 seconds to see the please wait.... remark. other subject area catalogs also available. koeltz Scientific Books -- Pamela Burns-Balogh Koeltz Scientific Books USA Champaign, IL 61821 From owner-mycology@net.bio.net Sat Feb 11 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.cac.psu.edu!news.pop.psu.edu!hudson.lm.com!newsfeed.pitt.edu!uunet!uunet.ca!uunet.ca!news.compulink.com!ninja From: ninja@cml.com (Joe Sommer) Newsgroups: bionet.mycology Subject: Mediums for Pleurotus growth Date: 12 Feb 1995 04:29:56 GMT Organization: ComputerLink Online Inc. Lines: 17 Message-ID: <3hk2s4$dto@news.compulink.com> NNTP-Posting-Host: cml.com X-Newsreader: TIN [version 1.2 PL2] Hello, I am going to conduct an experiment that deals with growing Pleurotus ostreatus on different types of substrates ie, cellulose, plastic, plywood, tripple mix soil, and so on. I want to see which medium provides the greatest yield measured by net dry/wet weight measures. Any suggestions would be appreciated in terms of the methodology and previous success on the above mentioned substrates. Joe --------------------------------------------------------------------- : ComputerLink Online Inc. Realms of Despair! : : (416)233-5410 telnet mud.compulink.com 4000 : : 106 lines, 300-28,800 bps endless medieval enjoyment! : : : : Join our International Teleconference --> chat.compulink.com 9000 : --------------------------------------------------------------------- From owner-mycology@net.bio.net Sun Feb 12 22:00:00 1995 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!usc!nic-nac.CSU.net!cello.gina.calstate.edu!cello.gina.calstate.edu!not-for-mail From: stweiss@cello.gina.calstate.edu (Steven H. Weiss) Newsgroups: bionet.mycology Subject: Cooking Mushrooms? Date: 13 Feb 1995 07:36:28 -0800 Organization: GINA and CORE+ Services of The California State University Lines: 6 Message-ID: <3hnu9s$jvp@cello.gina.calstate.edu> NNTP-Posting-Host: cello.gina.calstate.edu Does anyone have any suggestions for cookbooks that specialize in cooking wild mushrooms? Also, are there recipes anywhere out there on the Net? Thanks, Steve Weiss From owner-mycology@net.bio.net Sun Feb 12 22:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!swrinde!howland.reston.ans.net!torn!news.unb.ca!peinet.pe.ca!peinet.pe.ca!not-for-mail From: stewart@bud.peinet.pe.ca (Paul Stewart) Newsgroups: bionet.mycology Subject: re: metal uptake by fungi Date: 13 Feb 1995 08:47:21 -0400 Organization: PEINet, Charlottetown, Prince Edward Island, Canada Lines: 32 Sender: stewart@bud.peinet.pe.ca Distribution: world Message-ID: <3hnkcp$qr2@bud.peinet.pe.ca> NNTP-Posting-Host: bud.peinet.pe.ca Dear Rosemary, In response to your request for references on heavy metal uptake, first assume that the average water content of a fresh mushroom is 85%. This means that dried preparations will have about 10X the amount of contaminant reported for fresh. However, most tox. data on heavy metals is expressed as %dry matter. Here are a few references that may be helpful: (refs marked by a "*" are in my files...if you can't find a copy I can mail you one, if you don't mind the wait!) * Yasui, A., Tsutsumi, C., Takasaki, M., and T. Mori, 1988. (text in Japanese, abstract and tables in english) Absorption of elements from heavy-metals-containing culture media by oyster mushroom (P. ostreatus) NIPPON SHOKUHIN KOGYO GAKKAISHI (J.Jap.Soc.FoodSci.Tech) 35(3):160-165. (Cu, Cd, Pb, Ge...only Cd was uptaken, higher in cap than stem)(Nat Food Res Inst, Min Agri, For, & Fish, 2-1-2, Kannondai, Tsukuba-shi, Ibaraki, 305, Japan) Strmiskova, G., Strimiska, F., and J. Dubravicky, 1992. Mineral composition of oyster mushroom, NAHRUNG: FOOD 36(2):210-212. (G. Strm. @ Slovak Tech Univ, Fac Chem Technol, Radlinskeho Ul 9, CS-81237 Bratislava, Czechoslovakia) Mandic, ML, Grgic, J, Grgic, Z., and M. Seruga, 1992. The natural levels of aluminum, cadmium, and lead in wildlife mushrooms in Eastern Croatia, DEUTSCHE LEBENSMITTEL - RUNDSCHAU 88(3):76-77 (ML Mandic, Univ. Osijek, Fac Food Technol, YU-54000, Osijek, Yugoslavia Hope these are of some help. There are probably more recent ones (ie-from countries that still exist!); try looking in Food Science and Technology Abstracts, or contact Leatherhead in England for a complete search (they're pricy though thorough!)...Paul From The Great White Northeast. From owner-mycology@net.bio.net Sun Feb 12 22:00:00 1995 Path: biosci!adam.cc.sunysb.edu!news.sprintlink.net!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!msunews!harbinger.cc.monash.edu.au!aggedor.rmit.EDU.AU!s9104735 From: s9104735@bf.rmit.edu.au (Rosemary Waghorn) Newsgroups: bionet.mycology Subject: Info on metal uptake by fungi wanted Date: 13 Feb 1995 01:39:52 GMT Organization: Royal Melbourne Institute of Technology, Melbourne, Australia. Lines: 14 Message-ID: <3hmd98$hr2@aggedor.rmit.EDU.AU> NNTP-Posting-Host: otto.bf.rmit.edu.au NNTP-Posting-User: s9104735 Summary: metal uptake by fungi Keywords: help fungi metal X-Newsreader: NN version 6.5.0 (NOV) Does anyone know of any information about heavy metal (especially lead) uptake of dried preparations of fungi, in heavy metal contaminated soils or streams? Any book or journal references would be appreciated. Or about anything related. Thanks ..Rosemary.. -- -------------------------------------------------------------------------- Ladyhawke Practise random kindness and sensless acts s914735@otto.bf.rmit.oz.au of beauty. From owner-mycology@net.bio.net Sun Feb 12 22:00:00 1995 Newsgroups: bionet.mycology Path: biosci!rutgers!gatech!swrinde!howland.reston.ans.net!news.sprintlink.net!uunet!newsflash.concordia.ca!news.mcgill.ca!clouso.crim.ca!athena.ulaval.ca!ophiostoma.rsvs.ulaval.ca!user From: Louis.bernier@sbf.ulaval.ca (Louis Bernier) Subject: infection of fungi by protozoa-thanks Content-Type: text/plain; charset=ISO-8859-1 Message-ID: Followup-To: infection,of,fungi,by,protozoa Sender: news@athena.ulaval.ca Nntp-Posting-Host: ophiostoma.rsvs.ulaval.ca Content-Transfer-Encoding: 8bit Organization: Universite Laval Mime-Version: 1.0 Date: Mon, 13 Feb 1995 15:00:34 GMT Lines: 7 My previous query for information on how to rid fungal cultures of protozoa elicited an unexpectedly high number and diversity of answers from members of the mycology and forest pathology electronic networks. My thanks to all of you who contributed information. I will post the results we have obtained following application of the various protocols. Stay tuned. Louis Bernier From owner-mycology@net.bio.net Sun Feb 12 22:00:00 1995 Path: biosci!rutgers!gatech!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!caen!hearst.acc.Virginia.EDU!portal.gmu.edu!osf1.gmu.edu!hkilpatr From: hkilpatr@osf1.gmu.edu (HENRY E. KILPATRICK JR.) Newsgroups: bionet.mycology Subject: Re: Cooking Mushrooms? Date: 13 Feb 1995 23:00:03 GMT Organization: George Mason University, Fairfax, Virginia, USA Lines: 15 Message-ID: <3hoo9j$666@portal.gmu.edu> References: <3hnu9s$jvp@cello.gina.calstate.edu> NNTP-Posting-Host: osf1.gmu.edu Mime-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit X-Newsreader: TIN [version 1.2 PL2] Steven H. Weiss (stweiss@cello.gina.calstate.edu) wrote: : Does anyone have any suggestions for cookbooks that specialize : in cooking wild mushrooms? Also, are there recipes anywhere : out there on the Net? : : Thanks, : Steve Weiss -- The best one I've seen is written by Jack Czarnecki (sp?), who own's Joe's Restaurant in Reading, PA. I think the name of the book is something like Joe's Book of Mushroom Cookery. I have the book at home, so e-mail me if you need the exact name. Buddy Kilpatrick From owner-mycology@net.bio.net Sun Feb 12 22:00:00 1995 Path: biosci!ACS.UCALGARY.CA!bradbury From: bradbury@ACS.UCALGARY.CA Newsgroups: bionet.mycology Subject: RAPD primers for ecto's Date: 13 Feb 1995 13:32:46 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 4 Sender: daemon@net.bio.net Distribution: world Message-ID: <9502132133.AA30520@acs5.acs.ucalgary.ca> NNTP-Posting-Host: net.bio.net Can anyone advise me as to which RAPD primers maybe useful for amplifying fungal DNA from colonized ectomycorrhizal root tips (Lodgepole pine)? Any advice will be greatly appreciated. Thanks. From owner-mycology@net.bio.net Mon Feb 13 22:00:00 1995 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!swrinde!howland.reston.ans.net!nntp.crl.com!crl2.crl.com!not-for-mail From: kasplash@crl.com (Bob Galvan) Newsgroups: bionet.mycology Subject: Re: Cooking Mushrooms? Date: 13 Feb 1995 19:54:39 -0800 Organization: CRL Dialup Internet Access (415) 705-6060 [login: guest] Lines: 20 Message-ID: <3hp9hv$5i7@crl2.crl.com> References: <3hnu9s$jvp@cello.gina.calstate.edu> <3hoo9j$666@portal.gmu.edu> NNTP-Posting-Host: crl2.crl.com I know of two good books on wild mushroom cookery. 1> Wild About Mushrooms Cook book of the San Francisco Mycological Society I forget the author, (if even listed) and it is out of print, but I found it in my local library. 2> A Passion for Mushrooms kinda guessing on the author's name here - Paolo Ciccini he has several other books out, Passion for Pasta, Passion for etc... This book is loaded with beatiful, inspiring photos of finished mushroom dishes in addition to photos (some obviously staged) of wild mushrooms growing in the forest. Highly recommended. Recipes too. European perspective. Seasons will not match ours at all. Bob Galvan kasplash@crl.com From owner-mycology@net.bio.net Mon Feb 13 22:00:00 1995 Path: biosci!PANIX.COM!gyetter From: gyetter@PANIX.COM (Gene Yetter) Newsgroups: bionet.mycology Subject: The Mycologia Index, Vols. 1-58 Date: 13 Feb 1995 20:24:49 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 38 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Regular mycology newsgroup readers may recall the announcement I posted in December 1994 in which I sought volunteers to help transcribe the Mycologia Index, Vols. 1-58, to disk so that it could be made available online at the Telnet site, fungi.ars-grin.gov. I will scan the 1100-page Index and farm out to volunteers the task of cleaning-up and proofing of the scanner output. The project is still on track. Of the entire index, the first section, Fungus Taxa, through p. 514, has been scanned, broken up into blocks of pages and assigned to volunteers. About half of that number of pages have been cleaned up and e-mailed back to me. We're about a quarter of the way home. The individuals who have returned cleaned-up scanner output to date include: Felix (fgrun@mail.med.cornell.edu>), Kathie Hodge (Cornell University), Fred Rhoades (Bellingham, WA), Leo J. Van Griensven (The Netherlands), Brian McNett (Bremerton, WA), Richard Woodside (Canada) and Eugene Varney, Ph.D. (Rutgers University). Leo is the runaway leader with 120 pages returned. Quite an international cooperative effort! Several individuals have agreed to assist who have not yet been sent scanner output. This situation will be corrected as soon as I can devote more time to scanning. Additional volunteers will be needed as we swing into the Host Index and Subject Index sections. Many thanks to those who have participated or are still working on their sections. Please communicate with me if you missed the earlier posting and would like to know more. Gene Yetter New Jersey Myc. Assoc. & New York Myc. Society Reply to: gyetter@panix.com From owner-mycology@net.bio.net Mon Feb 13 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!gatech!news-feed-1.peachnet.edu!ukma!seqanal.mi.uky.edu!staben From: staben@seqanal.mi.uky.edu (Chuck Staben) Newsgroups: bionet.mycology Subject: G-protein effectors Date: 14 Feb 1995 02:37:57 GMT Organization: University of Kentucky, Dept. of Math Sciences Lines: 8 Message-ID: <3hp525$2n4@t2.mscf.uky.edu> NNTP-Posting-Host: seqanal.mi.uky.edu X-Newsreader: TIN [version 1.1 PL9] I wonder whether contributors to this forum might know which G-protein effectors are effective in either activating or inactivating the G-alpha signal transduction pathway in intact fungi. Although there are a million inhibitors used with G-proteins, and a lot of genetic work on S. cerevisiae, it is not clear to me how best to find this specific info. P.S. This is not a Neurospora-related question, but I have not deserted the one true organism--as readers of previous queries have queried From owner-mycology@net.bio.net Mon Feb 13 22:00:00 1995 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!swrinde!howland.reston.ans.net!news.sprintlink.net!uunet!in1.uu.net!olivea!nntp-hub.barrnet.net!decwrl!tribune.usask.ca!quartz.ucs.ualberta.ca!rover.ucs.ualberta.ca!news.ucalgary.ca!pc12.mid.ucalgary.ca!user From: cmody@acs.ucalgary.ca (Dr. C. Mody) Newsgroups: bionet.mycology Subject: Do I have cell wall fraction of yeast Date: Mon, 13 Feb 1995 18:13:28 -0700 Organization: MID Lines: 17 Message-ID: NNTP-Posting-Host: pc12.mid.ucalgary.ca Can anybody tell me how to be resonably sure that I have separated the cell wall and cytosolic fractions of Cryptococcus neoformans. C. neoformans was grown in Sabs broth, killed with heat (autoclaved), washed and about 10^9 organisms placed in a glass bead mill [Bead Beater®] (volume of organisms 75 ml, beads 100 ml, buffer to fill chamber). Rotated one minute on, one minute off for 60 minutes on ice. Disruption was complete by microscopic exam. A slow spin was done to remove debris. The preparation was spum at 13,000 xg for 30 minutes, and the pellet and supernatant was collected. My reading would indicate that the pellet should contain the cell wall, the supernatant the cytosol. How can I be more sure? Is there a simple assay that can be done to tell that my cell wall preparation has minimal contamination with cytosolic proteins and vice versa? Thanks Chris Mody cmody@acs.ucalgary.ca From owner-mycology@net.bio.net Mon Feb 13 22:00:00 1995 Path: biosci!CS.Arizona.EDU!news.Arizona.EDU!nectria.agmarley.arizona.edu!user From: qwu@ag.arizona.edu (Qindong Wu) Newsgroups: bionet.mycology Subject: phyophthora DNA Followup-To: bionet.mycology Date: Mon, 13 Feb 1995 17:07:11 +0100 Organization: University of Arizona Lines: 14 Message-ID: NNTP-Posting-Host: nectria.agmarley.arizona.edu A grad student in our department wants to bring samples of phytophthora into the states from Mexico, but realizes that live samples will not be permitted. Due to the nature of the project he is conducting, it will not be possible to extract DNA while in Mexico. What we have discussed, and what I am hoping to find out by making this posting, is whether it is possible to preserve tissue, grown axenically, in ethanol and bring it back for DNA extraction. Any advice would be greatly appreciated and can be forwarded to me or posted here. Thanks, Kevin McCluskey mccluske@ag.arizona.edu From owner-mycology@net.bio.net Mon Feb 13 22:00:00 1995 Path: biosci!CSC.ALBANY.EDU!pb019 From: pb019@CSC.ALBANY.EDU (Partha Banerjee) Newsgroups: bionet.mycology Subject: (none) Date: 14 Feb 1995 08:49:13 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <199502141649.LAA05325@sarah.albany.edu> NNTP-Posting-Host: net.bio.net It would be very nice if we could start a mycology job bulletin board on our newsgroup. It would also be worthwhile if we could start a column on why mycology jobs are so scarce and how we can create new jobs in mycology. The potential role of MSA and other like organizations and our schools and faculties in job-related matters should also be discussed. From owner-mycology@net.bio.net Mon Feb 13 22:00:00 1995 Newsgroups: bionet.mycology Path: biosci!rutgers!gatech!howland.reston.ans.net!swrinde!cs.utexas.edu!news.sprintlink.net!peernews.demon.co.uk!demon!gram.demon.co.uk!user From: gren@gram.demon.co.uk (Gareth Renowden) Subject: Re: Cooking Mushrooms? X-Nntp-Posting-Host: gram.demon.co.uk Message-ID: Sender: news@demon.co.uk (Usenet Administration) Organization: Publishing consultant References: <3hnu9s$jvp@cello.gina.calstate.edu> <3hoo9j$666@portal.gmu.edu> <3hp9hv$5i7@crl2.crl.com> Date: Tue, 14 Feb 1995 16:07:24 GMT Lines: 18 In article <3hp9hv$5i7@crl2.crl.com>, kasplash@crl.com (Bob Galvan) wrote: > 2> A Passion for Mushrooms > kinda guessing on the author's name here - Paolo Ciccini > he has several other books out, Passion for Pasta, Passion for etc... He's called Antonio Carluccio. And yes, it is very good. Another good one - over here, anyway - is The Mushroom Feast by Jane Grigson, a very famous UK cookery writer. -- Gareth Renowden l Publishing & marketing consultant l "I don't know what I want, Voice +44 (0)181 241 4852 l but I want it NOW!" Fax +44 (0)181 943 4294 l Sir Henry Rawlinson e-mail gren@gram.demon.co.uk l (c) Viv Stanshall From owner-mycology@net.bio.net Mon Feb 13 22:00:00 1995 Path: biosci!SUMMA.TAMU.EDU!dje0282 From: dje0282@SUMMA.TAMU.EDU (Dan Ebbole) Newsgroups: bionet.mycology Subject: the one true organism? Date: 14 Feb 1995 06:53:04 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: <199502141452.GAA09734@net.bio.net> NNTP-Posting-Host: net.bio.net Chuck Staben wrote: "P.S. This is not a Neurospora-related question, but I have not deserted the one true organism--as readers of previous queries have queried" Chuck, while I realize this is humor, there are others who actually could be offended by the "snobbishness" of the comment. Neurospora has some wonderful advantages and some disadvantages relative to other fungal systems for examining various aspects of fungal biology. I just want to make sure that everyone understands that Neurosporologists generally have great respect for other fungal systems. I know Chuck feels the same way and I'd hate for his comment to be misunderstood. It is most distasteful to send a message about politically correct statements regarding fungi-but here it is. ------------------------ Dr. Daniel Ebbole dje0282@summa.tamu.edu Dept. of Plant Pathology and Microbiology Texas A&M University College Station, TX 77843-2132 From owner-mycology@net.bio.net Mon Feb 13 22:00:00 1995 Path: biosci!rutgers!gatech!howland.reston.ans.net!news.sprintlink.net!peernews.demon.co.uk!btnet!uknet!strath-cs!st-and!Aberdeen!gen066 From: gen066@nof.abdn.ac.uk (b.morris) Newsgroups: bionet.mycology Subject: Re: calcium in fungi Date: 14 Feb 1995 13:23:32 GMT Organization: University of Aberdeen, Scotland Lines: 21 Distribution: world Message-ID: <3hqask$924@nof.abdn.ac.uk> References: <9502072052.AA01079@mani.cbs.umn.edu> <3hgh2h$avl@dns1.NMSU.Edu> NNTP-Posting-Host: bio.if2.abdn.ac.uk X-Newsreader: TIN [version 1.2 PL2] <9502072052.AA01079@mani.cbs.umn.edu> nora@molbio.cbs.umn.edu writes: : >I would appreciate some help in designing/assessing experiments to : >determine cytoplasmic calcium levels in Neurospora crassa or another : >fungus. Specific questions are: : > : > (1) Does the esterified fura-2 work in Neurospora for :.............. I can't help with the specifics but you may find some food for thought in the following papers :- Knight, H. et al. (1993) Confocal microscopy of living fungal hyphae microinjected with Ca2+-sensitive fluorescent dyes. Mycological Research 97(12), 1505-1515 Read, N.D. et al. (1992) Imaging and measurement of cytosolic free calcium in plant and fungal cells. Journal of Microscopy Vol 166, Part 1 April 1992, 57-86 Mike Morris @ Aberdeen, Scotland From owner-mycology@net.bio.net Tue Feb 14 22:00:00 1995 Path: biosci!agate!library.ucla.edu!csulb.edu!nic-nac.CSU.net!usc!howland.reston.ans.net!