From owner-mycology@net.bio.net Wed Mar 01 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.sprintlink.net!everest.pinn.net!everest!tiger From: tiger@pinn.net (Rusty Lynch) Newsgroups: bionet.mycology Subject: mushrm gifs Date: 2 Mar 1995 21:23:13 GMT Organization: Pinnacle Online - Internet access for Hampton Roads, Virginia - 498-3889 Lines: 7 Message-ID: <3j5d01$ggs@everest.pinn.net> NNTP-Posting-Host: everest.pinn.net X-Newsreader: TIN [version 1.2 PL2] -- =:|\Pinnacle Online - Open and dynamic Internet services:= =:|o\. | | | ______ := =:|_/||\ ||\ | |\.| __ | Voice: 804/498.3889 email: info@pinn.net:= =:| || \|| \| o | \| \_ .| Data: 804/498.9762 login: guest or new := From owner-mycology@net.bio.net Wed Mar 01 22:00:00 1995 Path: biosci!newshost.lanl.gov!ncar!gatech!howland.reston.ans.net!Germany.EU.net!EU.net!dkuug!eunet.no!nuug!nac.no!trane.uninett.no!astfgl.edb.tih.no!pc013.kjemi.tih.no!bjorn From: bjorn@ik.hist.no (Bjoern Arne Naess) Newsgroups: bionet.general,bionet.mycology,bionet.plants,bionet.protista,sci.bio,sci.microbiology,sci.biotechnology Subject: Mushrooms repellent/antagonism of insects and bacteria ?? Date: Thu, 2 Mar 1995 13:27:29 Organization: institutt for Kjemi Lines: 20 Message-ID: NNTP-Posting-Host: pc013.kjemi.tih.no X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Xref: biosci bionet.general:13893 bionet.mycology:1656 bionet.plants:5840 bionet.protista:259 sci.bio:14636 Hi !! We are working with mycorrhiza and mushrooms in forest ecosystems. In particular we are interested in the combination of Cantharellus and Boletus with Picea abies and Betula sp. In conection with this we are interested in the ability of e.g. Cantharellus cibarius to repell most insects or at least to avoid predation. Also antagonistic properities against bacterias is of our interest. We look for information concerning chemicals or physiological properterities on theese matters. We search information both for practical use in our work and for the purpose of building a Biotechnology web. Per-Odd Eggen and Bjoern Arne Naess Please answere to newsgroup or by E.mail to bjorn@ik.hist.no Thank You in advance !! From owner-mycology@net.bio.net Wed Mar 01 22:00:00 1995 Path: biosci!rutgers!gatech!swrinde!howland.reston.ans.net!news.cac.psu.edu!news.tc.cornell.edu!travelers.mail.cornell.edu!newstand.syr.edu!kong.syr.edu!griffin From: griffin@kong.syr.edu (David H. Griffin) Newsgroups: bionet.mycology Subject: Re: HELP! basidiomycete recognition systems Date: 1 Mar 1995 15:04:32 GMT Organization: SUNY ESF, Syracuse, NY Lines: 70 Sender: David Griffin Message-ID: <3j22e0$9a6@newstand.syr.edu> References: <3iuah1$3dk@news.compulink.com> NNTP-Posting-Host: kong.syr.edu >Hello, I need some information on how mating type compatability, somatic >compatability and intersterility function in basidiomycete fungi. I >would appreciate any advice about how these systems operate, does the Recent reviews: Casselton, L. A. and U. Kues. 1994. Mating-type genes in Homobasidiomycetes. Pp. 307-321 In J. G. H. Wessels and F. Meinhardt (Eds.), Growth, differentiation and sexuality. Springer-Verlag, Berlin. Kamper, J., M. Bolker and R. Kahmann. 1994. Mating-type genes in Heterobasidiomycetes. Pp. 323-332 In J. G. H. Wessels and F. Meinhardt (Eds.), Growth, differentiation and sexuality. Springer-Verlag, Berlin. Esser, K. and R. Blaich. 1994. Heterogenic incompatibility in fungi. Pp. 211-232 In J. G. H. Wessels and F. Meinhardt (Eds.), Growth, differentiation and sexuality. Springer-Verlag, Berlin. >current literature suggest that they are all independent of one another? >how do they function in terms of genetic loci? must they be different or >similar to produce a successful mating pair? These processes are genetically independent, but physiologically they interact. Somatic incompatibility in Hymenomycetes apparently functions only among dicaryons, and therefore has little impact on mating per se. This is different from Ascomycetes in one respect and similar in another, namely, somatic incompatibility in Asco's occurs between monocaryons, but does not effect mating between trichogyne and whatever antheridial structure is presented. Intersterility phenomena clearly override the mating-type loci, entirely or partially depending on the fungus and what genotypes are involved. >Also does somatic (vegetative) incompatability seem to be an artifactual >phenomenon? or does it serve a true functional role in segregating >members within a species to recognize only themselves in the natural >environment? Rayner has discussed this in considerable detail. I doubt very much that this is artifactual. Rayner, A. D. M., D. Coates, A. M. Ainsworth, T. J. H. Adams, E. N. D. Williams and N. K. Todd. 1984. The biological consequences of the individualistic mycelium. Pp. 509-540 In D. H. Jennings and A. D. M. Rayner (Eds.), The ecology and physiology of the fungal mycelium. Cambridge Univ Press, Cambridge. Rayner, A. D. M. 1991. The Phytopathological Significance of Mycelial Individualism. Annu. Rev. Phytopathol. 29: 305-323. >Any advice concerning the above systems and how they interact with one >another would be appreciated. Also any opinions on the function of >somatic compatability in the wild would also be appreciated. I hope that this is helpful. David H. Griffin Department of Environmental & Forest Biology 350 Illick Hall College of Environmental Science and Forestry One Forestry Drive Syracuse NY 13210-2788 e-mail: griffin@mailbox.syr.edu From owner-mycology@net.bio.net Wed Mar 01 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.cac.psu.edu!psuvm!djr4 Organization: Penn State University Date: Thu, 2 Mar 1995 11:52:26 EST From: Mushroom Research Lab Message-ID: <95061.115226DJR4@psuvm.psu.edu> Newsgroups: bionet.mycology Subject: Sphaerobolus Lines: 3 Anyone out there have any info (recent or otherwise) on the Artillery Fungus--- ---------Sphaerobolus spp.?????? I just did a quick data search and didn't find much. Any help would be appreciated.........Britt From owner-mycology@net.bio.net Wed Mar 01 22:00:00 1995 Path: biosci!MANI.CBS.UMN.EDU!npv From: npv@MANI.CBS.UMN.EDU (Nora Plesofsky-Vig) Newsgroups: bionet.mycology Subject: re:mito membrane protein fractions Date: 2 Mar 1995 14:51:35 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: <9503022248.AA01658@mani.cbs.umn.edu> Reply-To: nora@biosci.cbs.umn.edu NNTP-Posting-Host: net.bio.net I have not actually subfractionated mitochondria, but here are some references for the published procedures: Sottocasa GL et al., J. Cell Biol. 32 (1967):415-436 Riezman H et al., EMBO J. 2 (1983): 1105-1111 Freitag H et al., Eur. J. Biochem: 126 (1982): 197-202 " " : 123 (1982): 629-636 The protein content of the inner mitochondrial membrane is apparently much greater than that of the outer membrane. You could make submitochondrial particles (by sonication) to separate the membranes from the matrix proteins. Nora Plesofsky-Vig From owner-mycology@net.bio.net Thu Mar 02 22:00:00 1995 Path: biosci!CUTTER.SHIP.EDU!as5470 From: as5470@CUTTER.SHIP.EDU (Alyn Leigh Scritchfield) Newsgroups: bionet.mycology Subject: Paracoccidioides brasiliensis Date: 2 Mar 1995 16:54:36 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I am a fourth year student researching material for a presentation related to Paracoccidioides brasiliensis. Would anyone be able to provide me with or direct me to a good source of information on this fungus and its related pathology? Thank you. as5470@cutter.ship.edu. From owner-mycology@net.bio.net Thu Mar 02 22:00:00 1995 Path: biosci!CUTTER.SHIP.EDU!as5470 From: as5470@CUTTER.SHIP.EDU (Alyn Leigh Scritchfield) Newsgroups: bionet.mycology Subject: subscription Date: 2 Mar 1995 16:48:15 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 6 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Mycology administrator: I would like to susbscribe to bionet mycology newsgroup. Thank you. as5470@cutter.ship.edu From owner-mycology@net.bio.net Thu Mar 02 22:00:00 1995 Path: biosci!sasa.gov.uk!burns From: burns@sasa.gov.uk (Robert Burns) Newsgroups: bionet.mycology Subject: test....ignore Date: 2 Mar 1995 02:32:17 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: Robert Burns Monoclonal Antibody Unit Scottish Agricultural Science Agency East Craigs Edinburgh Scotland burns@sasa.gov.uk From owner-mycology@net.bio.net Thu Mar 02 22:00:00 1995 Path: biosci!ESSEX.HSC.COLORADO.EDU!claudes From: claudes@ESSEX.HSC.COLORADO.EDU (Claude Selitrennikoff) Newsgroups: bionet.mycology Subject: Postdoctoral positions available Date: 3 Mar 1995 10:51:31 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Three postdoctoral positions are available beginning July 1 to study the molecular biology of cell-wall assembly of Neurospora crassa. These positions are funded from a 3-year NIH RO1. Requirements for two postions include experience with fungal growth and molecular biology of filamentous fungi, including screening libraries by sibselection, the isolation and cloning of genes and cDNAs. Some experience with Candida albicans a plus. The other postion will involve the purification of an integral membrane enzyme complex, (1,3)B-glucan synthase. Experience concerning protein purification a must. Please reply to: Claude P. Selitrennikoff Professor University of Colorado Health Sciences Center Denver CO 80262 I will be at the Fungal Genetics Meeting at Asilomar in late March. From owner-mycology@net.bio.net Thu Mar 02 22:00:00 1995 Path: biosci!DTPAX2.NCIFCRF.GOV!SNYDER From: SNYDER@DTPAX2.NCIFCRF.GOV Newsgroups: bionet.mycology Subject: sphaerobolus reply Date: 3 Mar 1995 06:40:34 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 6 Sender: daemon@net.bio.net Distribution: world Message-ID: <950303094316.2022be84@dtpax2.ncifcrf.gov> NNTP-Posting-Host: net.bio.net There is an article by G.T. Ingolt "Spaerobolus - story of a fungus" in Trans. Brit. Mycol. Soc. Vol.58, p. 179-195, 1972. Also, you could try looking in one of the six volumes of A.H.R. Buller's "Research on Fungi" 1922. V. Snyder From owner-mycology@net.bio.net Thu Mar 02 22:00:00 1995 Path: biosci!ns1.faseb.org!darwin.sura.net!gatech!swrinde!howland.reston.ans.net!vixen.cso.uiuc.edu!prairienet.org!peburnsx From: peburnsx@prairienet.org (Pamela E. Burns-Balogh) Newsgroups: bionet.mycology Subject: mycology catalog is over 1MB-can't email Date: 3 Mar 1995 12:09:31 GMT Organization: University of Illinois at Urbana Lines: 10 Message-ID: <3j70tr$ojq@vixen.cso.uiuc.edu> NNTP-Posting-Host: firefly.prairienet.org we are responding to many users of this bulletin board who requested we send our catalog to them via email, our mycology catalog is 1133000+ in size and cannot be emailed to you. you can download this via any communications program from your modem 217-355-4532 takes 20 seconds to load our comm program so please wait until you see the please wait.. Pamela Burns-Balogh Koeltz Scientific Books USA -- Koeltz Scientific Books USA From owner-mycology@net.bio.net Fri Mar 03 22:00:00 1995 Path: biosci!IDPTV.IDBSU.EDU!pub907 From: pub907@IDPTV.IDBSU.EDU (Warren Rekow) Newsgroups: bionet.mycology Subject: Re: Predatorial Fungi Date: 4 Mar 1995 10:13:46 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net On march 4th egrunden@prairienet.org (Eric Grundein) asked: > I know that there is a fungi that traps nematodes by > forming a loop that constricts when a nematode passes > through. Does anyone know the name of this fungi? Eric, you may want to look up the following article: "Jekyll-Hyde Mushrooms" by George Barron in Natural History magazine, March 1992 This article describes fungal predation and includes a picture of the fungus Arthrobotrys capturing a nematode in a ring structure. You may also want to pose this question on the Micronet mail list operated by people at the University of Guelph in Canada. This university is cited in the above article. -- Warren Rekow (pub907@idptv.idbsu.edu) HC 79, Box 104, Melba ID 83641 Phone or fax: 208-896-4908 From owner-mycology@net.bio.net Fri Mar 03 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!msunews!harbinger.cc.monash.edu.au!news.cs.su.oz.au!metro!unsw.edu.au!usenet From: S.Yussof@unsw.EDU.AU (Shaharin Yussof) Newsgroups: bionet.mycology Subject: Re: MORAL'S Date: 4 Mar 1995 07:17:55 GMT Organization: Dept. of Safety Science, UNSW Lines: 15 Message-ID: <3j9473$rn5@mirv.unsw.edu.au> References: <3ir63n$sj9@newsbf02.news.aol.com> <3iua4u$grf@crl7.crl.com> NNTP-Posting-Host: 149.171.28.200 Mime-Version: 1.0 X-Newsreader: WinVN 0.93.14 In article <3iua4u$grf@crl7.crl.com>, kasplash@crl.com says... > >Well let's start with habitat, like *where are you*? >Then maybe someone here can refer you to a veteren picker. ^ >and then you'll buy Mushrooms Demystifyed, by David Arora and you'll ^ >Then you'll learn to replace the a with an e and get morels. Don't correct others until you can correct yourself! Shah From owner-mycology@net.bio.net Fri Mar 03 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!prairienet.org!egrunden From: egrunden@prairienet.org (Eric Grunden) Newsgroups: bionet.mycology Subject: Predatorial Fungi Date: 4 Mar 1995 06:52:04 GMT Organization: University of Illinois at Urbana Lines: 15 Message-ID: <3j92mk$gko@vixen.cso.uiuc.edu> Reply-To: egrunden@prairienet.org (Eric Grunden) NNTP-Posting-Host: firefly.prairienet.org I know that there is a fungi that traps nematodes by forming a loop that constricts when a nematode passes through. Does anyone know the name of this fungi? Does it "feed" on a specific nematode? Could the fungi be used as a troubleshooter in a field exhibiting signs of soybean cyst nematode infection? = eg = -- * * * * * * * "An empty can, just like a mind, makes noisy senseless clatter. Full cans sit stoically behind, and contemplate the latter." - excerpted from "The Collective Works of Johnny Pokerface" - From owner-mycology@net.bio.net Fri Mar 03 22:00:00 1995 Path: biosci!bcm!news.msfc.nasa.gov!cs.utk.edu!gatech!howland.reston.ans.net!news.sprintlink.net!uunet!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: kmcfarl714@aol.com (KMcfarl714) Newsgroups: bionet.mycology Subject: avian use of marasmius mushrooms Date: 3 Mar 1995 21:09:34 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 4 Sender: root@newsbf02.news.aol.com Message-ID: <3j8i4u$kqt@newsbf02.news.aol.com> Reply-To: kmcfarl714@aol.com (KMcfarl714) NNTP-Posting-Host: newsbf02.mail.aol.com I am looking for any information anyone may have on birds using mushrooms, particularly marasmius spp., in nest construction. Any personal notes or tips on published material would be helpful. Please e-mail me with help. Thanks. From owner-mycology@net.bio.net Fri Mar 03 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!news.sprintlink.net!kaleka.seanet.com!usenet!not-for-mail From: meriah@seanet.com (Meriah Crawford) Newsgroups: bionet.mycology Subject: Re: black pepper Date: 4 Mar 1995 12:31:33 -0800 Organization: Seanet Online Services, Seattle WA Lines: 13 Message-ID: <3jain5$o6@kisa.seanet.com> References: NNTP-Posting-Host: kisa.seanet.com In article , Novo Nordisk Biotech wrote: >i have heard a rumor that black pepper is made black by processing >of green pepper with Glomerella cingulata. is this true? I read (somewhere) recently that black pepper is green pepper that has been "aged" and has had a fungus form on it naturally. I don't remember the name of the fungus, but my understanding is that "process" would definitely not be the right word. I may have read this in rec.foods.historic ?? Meriah... From owner-mycology@net.bio.net Sat Mar 04 22:00:00 1995 Path: biosci!agate!nntp-ucb.barrnet.net!well!peter From: peter@well.sf.ca.us (Peter Febbroriello) Newsgroups: bionet.mycology Subject: Plasmopara ??? Date: 5 Mar 1995 00:52:30 GMT Organization: The Whole Earth 'Lectronic Link, Sausalito, CA Lines: 4 Message-ID: <3jb20e$pvs@nkosi.well.com> NNTP-Posting-Host: well.sf.ca.us Looking for any info aiding in speciation of Plasmopara. reply to 2448141@mcimail.com or peter@well.sf.ca.us From owner-mycology@net.bio.net Sat Mar 04 22:00:00 1995 Newsgroups: bionet.mycology Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!agate!newsxfer.itd.umich.edu!gatech!udel!news.bluesky.net!news.sprintlink.net!alfa02.medio.net!netnews.nwnet.net!news.pfc.forestry.ca!PFC.Forestry.CA!RWINDER From: rwinder@PFC.Forestry.CA (Richard Winder) Subject: Re: MORAL'S X-Nntp-Posting-Host: pfc.pfc.forestry.ca Message-ID: Sender: news@news.pfc.forestry.ca (Usenet News) Reply-To: rwinder@PFC.Forestry.CA Organization: Forestry Canada (Pacific Forestry Centre) References: <3ir63n$sj9@newsbf02.news.aol.com>,<3j0aqs$12ts@usenetw1.news.prodigy.com> Date: Wed, 1 Mar 1995 17:53:02 GMT Lines: 20 In article <3j0aqs$12ts@usenetw1.news.prodigy.com>, VPGH51A@prodigy.com (Jim Berlstein) writes: >I also want to know where in Ca., so I can estimate the progress of Morel >season up the coast to portland and Seattle. Yes! If anyone could report the northward progress of morel fruiting in North America &/or the West Coast, it would really be a big help to those of us who have to plan dates for mushroom club forays (some members in my group are still not convinced there's even such thing as a morel- we seem to have hit all the wrong dates and places last year.) Tracking the chanterelle reports northward last fall in bionet.mycology proved to be very useful- we were able to hit the nail right on the head. I imagine that northward tracking would also benefit those further south who want to know how far they are into the season. -RSW RICHARD WINDER Title: Research Scientist Canadian Forest Service Phone: (604) 363-0773 Victoria, B.C. Internet: RWINDER@A1.PFC.Forestry.CA From owner-mycology@net.bio.net Sat Mar 04 22:00:00 1995 Path: biosci!agate!newsxfer.itd.umich.edu!gatech!udel!news.udel.edu!strauss.udel.edu!not-for-mail From: heytler@strauss.udel.edu (Peter Heytler) Newsgroups: bionet.mycology Subject: Re: black pepper Date: 5 Mar 1995 11:08:04 -0500 Organization: University of Delaware Lines: 26 Message-ID: <3jcnl4$1ps@strauss.udel.edu> References: <3jain5$o6@kisa.seanet.com> NNTP-Posting-Host: strauss.udel.edu In article <3jain5$o6@kisa.seanet.com>, Meriah Crawford wrote: >In article , >Novo Nordisk Biotech wrote: >>i have heard a rumor that black pepper is made black by processing >>of green pepper with Glomerella cingulata. is this true? > >I read (somewhere) recently that black pepper is green pepper that has >been "aged" and has had a fungus form on it naturally. mber >Meriah... That doesn't sound right to me, though there may be some type of condiment prepared that way. Common black pepper comes from a totally different plant, and two types are in far-apart plant families with different habitats. Ordinary black peppercorns are the dried small berries of a tropical vine (Piper nigrum). Green peppers - as well as the various other fleshy green, red and yellow peppers - are the fruit of various species of Capsicum, esp. C. frutescens. Red pepper seeds, of course, come from this type of pepper fruit. It's certainly possible that fungus-infected pepper-fruits could yield blackened seeds, which might be used somewhere; I don't know. --Peter Heytler From owner-mycology@net.bio.net Sun Mar 05 22:00:00 1995 Path: biosci!MANI.CBS.UMN.EDU!npv From: npv@MANI.CBS.UMN.EDU (Nora Plesofsky-Vig) Newsgroups: bionet.mycology Subject: lag in email transmission Date: 5 Mar 1995 16:13:20 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: <9503060009.AA00363@mani.cbs.umn.edu> Reply-To: nora@biosci.cbs.umn.edu NNTP-Posting-Host: net.bio.net Greetings: I am sending this message on March 5th to document for myself (and others who receive newsgroup messages as email) how long it takes to receive this message. I have noticed that there is a completely different group of messages posted on the bionet.mycology newsgroup (more recent, I assume) than I am receiving at roughly the same time by email. This was not the case for another newsgroup, where I had a good correspondence between the posted messages and the email I received. Is there a reason for this lag in the mycology group? Can anything be done about it? Nora Plesofsky-Vig From owner-mycology@net.bio.net Sun Mar 05 22:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!howland.reston.ans.net!news.cac.psu.edu!news.tc.cornell.edu!news.graphics.cornell.edu!newsstand.cit.cornell.edu!cu-dialup-0232.cit.cornell.edu!user From: mhg3@cornell.edu (Mike Griggs) Newsgroups: bionet.mycology Subject: Re: insect control Date: 4 Mar 1995 14:08:41 GMT Organization: Research Support Scientist/Entomologist Lines: 20 Sender: mhg3@cornell.edu (Verified) Message-ID: References: NNTP-Posting-Host: cu-dialup-0232.cit.cornell.edu I am a little late in responding to this but here is what I know. I work with a group doing research on entomopathogenic fungi. Our cryocollection contains approximately 4800 isolates of fungi collected from insects from around the world. There are supprisingly few collections from hymenopterans and very few indeed collected from the honey bee (Apis melifera). The one exception is ascosphaera apis the fungus that causes chalk brood. Then again why would one want to control the honey bee unless atempting to shift the resources to an endangered solitary bee. Removing local bee hives would have a greater affect and or bacillus larvae (foul brood). Hope this answers your question. Mike In article , Larry Jones wrote > I wanted to know if anyone knows if any parasitic fungi have been use to > control bees. (Apis). Any information will be appreciated. > Larry Jones -- Mike Griggs Entomologist/Apiculturist/Arborist From owner-mycology@net.bio.net Sun Mar 05 22:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!swiss.ans.net!prodigy.com!usenet From: VPGH51A@prodigy.com (Jim Berlstein) Newsgroups: bionet.mycology Subject: Re: Yellow Moral (sic) Date: 6 Mar 1995 00:12:41 GMT Organization: Prodigy Services Company 1-800-PRODIGY Lines: 27 Distribution: world Message-ID: <3jdk1p$uv8@usenetp1.news.prodigy.com> References: <01HNLL385O2Q8WY3LN@AXE.HUMBOLDT.EDU> NNTP-Posting-Host: inugap1.news.prodigy.com X-Newsreader: Version 1.2 In regards to some mushrooms making some people sick, as far as I know it is not uncommon. Many people eat Verpa Bohemica- I have eaten them without any bad effects. My wife wanted to try them, I parboiled them, I through out the water 3 times, I cooked the hell out of them. I allowed her to eat 6 small ones. She liked them and wanted more, but I would not allow it. Now she calls them "acher-makers" and will not eat them again. Also, beware of edible mushrooms from suburban lawns containing pesticides or herbacides or whatever. I never eat a new mushroom on the first find. I always try very small amounts. I only sample really good ones. I eat "old friends" without hesitation, like white and yellow chanterelles, true morels, rozites, hericium, boletus edulis and B. mirabilis, hydenums etc...but I do not serve them to others. I avoid mushrooms other's eat like Lepiota rachodes, sulfur shelves, honey mushrooms, lobster mushrooms, and all amanitas. There are no extra points for eating what you find, so eat what you like and eat what you know. Never eat a mushroom to prove something. - JIM BERLSTEIN VPGH51A@prodigy.com From owner-mycology@net.bio.net Sun Mar 05 22:00:00 1995 Path: biosci!rutgers!gatech!howland.reston.ans.net!news.sprintlink.net!uunet!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: mycochef@aol.com (MYCOCHEF) Newsgroups: bionet.mycology Subject: Re: Kombucha, Manchurian mushroom Date: 6 Mar 1995 14:57:12 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 6 Sender: root@newsbf02.news.aol.com Message-ID: <3jfpeo$6pq@newsbf02.news.aol.com> References: <3je3ss$411@dingo.cc.uq.oz.au> Reply-To: mycochef@aol.com (MYCOCHEF) NNTP-Posting-Host: newsbf02.mail.aol.com Paul Stamets wrote a great article for Mushroom, the Journal of Wild Mushrooming. Winter 1994-1995. check it out. quickly--kombucha is a mixture of yeasts and bacteria that has been around quite a while. Seems that today a lot of interest is being generated. here in Marin country , Ca. a lot of folks (usually old hippies) drink it faithfully. It "cures" grey hair and makes you feel young again, , , , From owner-mycology@net.bio.net Sun Mar 05 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.cac.psu.edu!psuvm!hgw1 Organization: Penn State University Date: Mon, 6 Mar 1995 10:49:04 EST From: Heather Treaster Message-ID: <95065.104904HGW1@psuvm.psu.edu> Newsgroups: bionet.mycology Subject: Grad. Assistantships Available Lines: 15 The Penn State Plant Pathology Department has several assistantships available for M.S. or Ph.D. students. Faculty programs include a diverse range of research in basic and applied plant pathology, including: field and greenhouse crop pathology, forest pathology, sustainable agriculture, molecular fungal taxonomy, mushroom culture and pathology, fungal genetics, epidemiology, virology, plant responses to environmental stress, air pollution effects to forest trees and ecosystems, root-specific metabolism, root-pathogen interactions, host-parasite interactions. Excellent facilities available for field work and biochemical/molecular biology research. Contact Dr. J. E. Ayers, Graduate Admissions Committee, Plant Pathology Department, 211 Buckhout Laboratory, University Park, PA 16802 (Phone: 814-865-7069; Fax: 814-863-7217; E-mail: htreaste@psupen.psu.edu). Affirmative Action/Equal Opportunity Employer; women and minorities encouraged to apply. From owner-mycology@net.bio.net Sun Mar 05 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!gatech!news-feed-1.peachnet.edu!news.duke.edu!eff!cs.umd.edu!news.umbc.edu!haven.umd.edu!purdue!mozo.cc.purdue.edu!levy.bio.purdue.edu!user From: kzeller@bilbo.bio.purdue.edu (K.A.Zeller) Newsgroups: bionet.mycology Subject: Re: black pepper Followup-To: bionet.mycology Date: 6 Mar 1995 15:54:42 GMT Organization: Biological Sciences Lines: 37 Distribution: world Message-ID: References: <3jain5$o6@kisa.seanet.com> <3jcnl4$1ps@strauss.udel.edu> NNTP-Posting-Host: levy.bio.purdue.edu In article <3jcnl4$1ps@strauss.udel.edu>, heytler@strauss.udel.edu (Peter Heytler) wrote: > > > > >I read (somewhere) recently that black pepper is green pepper that has > >been "aged" and has had a fungus form on it naturally. mber > >Meriah... > > That doesn't sound right to me, though there may be some type of > condiment prepared that way. Common black pepper comes from a totally > different plant, and two types are in far-apart plant families with > different habitats. > Ordinary black peppercorns are the dried small berries of a tropical > vine (Piper nigrum). Green peppers - as well as the various other > fleshy green, red and yellow peppers - are the fruit of various species > of Capsicum, esp. C. frutescens.[snip] > > --Peter Heytler Actually,... I think that the green peppercorns (yes these do exist) do come from a species of Piper (I'll have to look it up in a herb & spice book that I have at home to be sure) and that the black ones are just fermented and dried green pepercorns. I think you are confusing the two types of green "pepper". Sorry I can't add any input into the origional question; as to what specifically is done to make the green peppercorns into black ones. Kurt A. Zeller * -- One difficulty, of course, is that no Dept. of Biol. Sci * two students,... will agree entirely on the Purdue University-W. Lafayette* relative significance of the various * features involved. This, however, should There really is fun in fungi! * discourage no one: that one's own ideas * cannot be unequivocally proved to be * correct is a small price to pay for the * certainty that they will not be proved * entirely wrong.--Raper and Flexer, 1971. From owner-mycology@net.bio.net Sun Mar 05 22:00:00 1995 Path: biosci!COMPUSERVE.COM!70363.1442 From: 70363.1442@COMPUSERVE.COM ("Ted D. Conley") Newsgroups: bionet.mycology Subject: Unsubscribe request... Date: 5 Mar 1995 21:25:09 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: <950306052335_70363.1442_CHV63-2@CompuServe.COM> NNTP-Posting-Host: net.bio.net Sorry to do this to you all, but what is the address and code msg I send to send to get off this list?\ From owner-mycology@net.bio.net Sun Mar 05 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!gatech!news-feed-1.peachnet.edu!usenet.eel.ufl.edu!gmi!msunews!harbinger.cc.monash.edu.au!bunyip.cc.uq.oz.au!news From: M.Dale@botany.uq.oz.au (Michele Dale) Newsgroups: bionet.mycology Subject: Kombucha, Manchurian mushroom Date: 6 Mar 1995 04:43:08 GMT Organization: Botany Dept, Uni of Queensland Lines: 10 Message-ID: <3je3ss$411@dingo.cc.uq.oz.au> NNTP-Posting-Host: dale.botany.uq.oz.au X-Newsreader: WinVN 0.92.6+ I'm sure I should already know this, but there was an article in the weekend paper a while ago extolling the virtues of the Kombucha or Manchurian Mushroom. Apparently people drink a tea which amounts to drinking the culture filtrate, claimed to fix everything from arthritis to eczema. Out of interest can someone please tell me what this fungus is? Thanks in advance, Michele. From owner-mycology@net.bio.net Mon Mar 06 22:00:00 1995 Path: biosci!agate!ames!onramp.arc.nasa.gov!pioneer.arc.nasa.gov!watson From: watson@pioneer.arc.nasa.gov (John S. Watson - FSC) Newsgroups: bionet.mycology Subject: Re: Morels in the SF Bay Area? Date: 6 Mar 1995 22:08:05 GMT Organization: NASA Ames Res. Ctr. Mtn Vw CA 94035 Lines: 18 Distribution: ba Message-ID: <3jg145$sf6@onramp.arc.nasa.gov> References: <3htv7j$p33@onramp.arc.nasa.gov> NNTP-Posting-Host: pioneer.arc.nasa.gov In article <3htv7j$p33@onramp.arc.nasa.gov> watson@pioneer.arc.nasa.gov (John S. Watson - FSC) writes: > >Do morels occur in the bay area? Well, I get to answer my own question ... YES YES YES!!! :-) I just found (March 6, 1995) a couple yellow morels growing in the planter in the building across the street from mine. This is the first time I've ever found a morel in S.F. bay area. Previously I've found some in the Sierra (California) and Olympic peninsula (Washington). Now, how can I make a feast out of 2 morels? John S. Watson NASA Ames Research Center MS/243-9, Moffett Field, CA 94035 John S. Watson "Don't it always seem to go, that you don't know what you've got 'til it's gone ..." From owner-mycology@net.bio.net Mon Mar 06 22:00:00 1995 Path: biosci!sasa.gov.uk!burns From: burns@sasa.gov.uk (Robert Burns) Newsgroups: bionet.mycology Subject: test-ignore Date: 6 Mar 1995 08:59:26 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: test Robert Burns Monoclonal Antibody Unit Scottish Agricultural Science Agency East Craigs Edinburgh Scotland burns@sasa.gov.uk From owner-mycology@net.bio.net Mon Mar 06 22:00:00 1995 Path: biosci!EM.AGR.CA!REELEDERR From: REELEDERR@EM.AGR.CA ("Richard D. Reeleder") Newsgroups: bionet.mycology Subject: seed pathogens - reply Date: 7 Mar 1995 12:47:17 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Laura Hyatt lhyatt@mail.sas.upenn.edu wrote: >Does anyone know about any fungi that specialize on bringing an end >to the life of seeds in the soil? I am a seed bank ecologist and am >wondering about the effects of fungi on seed longevity. Also, does >anyone know about how one might go about measuring and >manipulating the abundance of hyphae in the soil? Many fungi will rot seeds; the prevalence of a particular fungal group as seed-rotting pathogens depends in part on the plant species, age of seed, soil type, temperature and moisture. There are many references on this subject; I would suggest Bruehl's text as a start: Bruehl, GW 1987. Soilborne Plant Pathogens. Macmillan Pub Co. For measuring fungal activity in the soil, there are also many techniques available. I suggest this review of techniques and approaches: Parkinson, D; Coleman, DC. 1991. Microbial Communities, Activity and Biomass. Ecosyst. Environ. 34:3-33. RD Reeleder reelederR@em.agr.ca From owner-mycology@net.bio.net Mon Mar 06 22:00:00 1995 Path: biosci!adam.cc.sunysb.edu!news.nysernet.net!news.sprintlink.net!howland.reston.ans.net!news.cac.psu.edu!news.pop.psu.edu!psuvax1!news.cc.swarthmore.edu!netnews.upenn.edu!mail1.sas.upenn.edu!lhyatt From: lhyatt@mail1.sas.upenn.edu (Laura Hyatt) Newsgroups: bionet.mycology Subject: seed pathogens Date: 7 Mar 1995 17:38:44 GMT Organization: University of Pennsylvania Lines: 16 Message-ID: <3ji5n4$t3m@netnews.upenn.edu> NNTP-Posting-Host: mail1.sas.upenn.edu X-Newsreader: TIN [version 1.2 PL2-upenn1.1] Does anyone know about any fungi that specialize on bringing an end to the life of seeds in the soil? I am a seed bank ecologist and am wondering about the effects of fungi on seed longevity. Also, does anyone know about how one might go about measuring and manipulating the abundance of hyphae in the soil? Thanks for your attention. Laura -- Laura Hyatt lhyatt@mail.sas.upenn.edu ------------------------------------------------------------------ Keep in mind the present you are constructing. It should be the future you want. ------------------------------------------------------------------ From owner-mycology@net.bio.net Mon Mar 06 22:00:00 1995 Path: biosci!CS.Arizona.EDU!math.arizona.edu!news.Arizona.EDU!ag!mccluske From: Kevin McCluskey Newsgroups: bionet.mycology Subject: Fungal Genetics Conference at Asilomar Date: Mon, 6 Mar 1995 17:39:27 -0700 Organization: The University of Arizona Lines: 93 Message-ID: NNTP-Posting-Host: ag.arizona.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII (I am posting this for Hans VanEtten. If anyone needs to contact his lab before the 13th, please contact me by E-mail. Kevin McCluskey) To individuals that will be attending the 18th Fungal Genetics Meeting at Asilomar on March 21 to 26: Below is the schedule for the workshop on "Pathogenicity and avirulence genes of plant pathogens: new and emerging techniques for their identification and isolation" which will take place on the afternoon of March 22. The objective of this workshop is to highlight approaches that allow the isolation of pathogenicity or avirulence genes without prior knowledge of the gene's biochemical function or activity. Some of the presenters will supply hand-outs of the techniques they will discuss. There is still some room on the program during the "general discussion periods" for additional presentations of approaches that are not listed on the program as well as time for others to share their experience with the listed techniques. If you have experience with a technique that has allowed you to isolate a pathogenicity or avirulence gene or experience with the listed techniques and would be willing to share that information, please contact me (Hans VanEtten) at email VANETTEN@CCIT.ARIZONA.EDU. Unfortunately, because of my travel plans, I will not be accessing my email until March 13. However, hopefully communications initiated at that date or shortly there after will still be in time to make arrangements for participation in the workshop. Workshop on "Pathogenicity and Avirulence genes of Plant Pathogens: New and Emerging Techniques for their Identification and Isolation." 1:30 Introduction - Hans VanEtten, University of Arizona. 1:35 "Introduction of the restriction enzyme- mediated integration (REMI) technique and experience with Magnaporthe grisea" - Jim Sweigard, DuPont. 1:55 "REMI-induced mutations in several toxin- producing fungi." - Olen Yoder, Cornell University. 2:10 Open to discussion on the REMI technique. 2:25 Transposons: "Toward the development of a gene-tagging system in Fusarium oxysporum" - Marie Josee Daboussi, Universite Paris Sud. 2:45 Open floor on use of transposons. 3:00 "Differential cDNA screening strategies for the identification of stage-specific gene expression in pathogenic fungi." - Nick Talbot, University of Exeter. 3:15 "Identification of pathogenicity genes in U. maydis by differential display" - Regina Kahmann, University of Munich. 3:30 "Identification of virulence genes by negative selection." - David Holden, Hammersmith Hospital, London. 3:45 Open to floor on approaches to isolate pathogenicity or avirulence genes by first isolating or identifying their transcripts or promoters. 4:00 Break. 4:15 "Determining if pathogenicity genes are on dispensable chromosomes." - Hans VanEtten, Univeristy of Arizona. 4:30 "Telomere-mediated chromosome breakage and analysis of pathogenicity determinants on dispensable chromosomes." - Corby Kistler, University of Florida. 4:45 Open to floor on techniques that take advantage of pathogenicity or avirulence genes being on dispensable genomic DNA. 5:00 "Mapping genes in Phytophthora infestans using bulked segregant analysis." - Howard Judelson, University of California, Riverside. 5:15 "Genetic and physical mapping with amplified fragment length polymorphisms (AFLPs) and bacterial artificial chromosomes (BACs)." - Richard Michelmore, University of California, Davis. 5:30 Open to floor for discussion of physical mapping strategies and general discussion. ************************************************************************** ** ** ** Kevin McCluskey Department of Plant Pathology ** ** Forbes Building Room 104 ** ** Phone: (602) 621-9390 University of Arizona ** ** FAX: (602) 621-9290 Tucson, AZ 85721 ** ** ** ************************************************************************** From owner-mycology@net.bio.net Tue Mar 07 22:00:00 1995 Newsgroups: bionet.mycology Path: biosci!rutgers!gatech!newsfeed.pitt.edu!dsinc!ub!netfs.dnd.ca!dgbt!nott!cunews!freenet.carleton.ca!FreeNet.Carleton.CA!au331 From: au331@FreeNet.Carleton.CA (Gary Cristall) Subject: Italian Mushroom Knives Message-ID: Sender: au331@freenet3.carleton.ca (Gary Cristall) Organization: The National Capital FreeNet, Ottawa, Ontario, Canada Date: Wed, 8 Mar 1995 05:58:02 GMT Lines: 8 Recently a friend returned from Italy with a mushroom knife. It has a hooked blade and a brush on the end for cleaning the little beasts. It also has a ruler on one side for measuring the harvest. I would like to know if you can buy them here, i.e. in North America. I checked with a local Italian knife store but they had never seen one. My friend paid about 40 Canadian dollars for the one she bought in Italy. Has anyone seen such a thing for sale? Thanks for any info. From owner-mycology@net.bio.net Tue Mar 07 22:00:00 1995 Path: biosci!rutgers!gatech!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!fnnews.fnal.gov!lakesis.fapesp.br!bee08.uspnet.usp.br!bee.uspnet.usp.br!lsvermes From: lsvermes@icb2.usp.br (Lucia M. Singer Vermes) Newsgroups: bionet.mycology Subject: RE: P. brasiliensis Date: 8 Mar 1995 10:05:29 GMT Organization: Universidade de Sao Paulo / Brasil Lines: 19 Message-ID: <3jjvh9$3l7@bee.uspnet.usp.br> NNTP-Posting-Host: biomed.icb2.usp.br X-Newsreader: TIN [version 1.2 PL2] uYou can find complete and updated information on Paracoccidioides brasiliensis and paracoccidioidoiomycosis in: FRANCO, M; LACAZ,C.S.; RESTREPO-MORENO,A. & DEL NEGRO, G. - Paracoccidioidomycosis. CRC PRESS (Boca Raton,FL) - 1994 The book cover several aspects of the disease and of the etiological agent written mostly by investigators that live in endemic areas of the disease and that are studying P. brasiliensis and paracoccidioidoiomycosis for many years. All the best Lucia Mary Singer Vermes Depto. de Imunologia - ICB - USP FAX (55) (11) 8130845 Fone (55) (11) 8187397 SAo Paulo - SP - Brazil From owner-mycology@net.bio.net Tue Mar 07 22:00:00 1995 Path: biosci!ESSEX.HSC.COLORADO.EDU!claudes From: claudes@ESSEX.HSC.COLORADO.EDU (Claude Selitrennikoff) Newsgroups: bionet.mycology Subject: Postdoctoral positions available Date: 8 Mar 1995 15:15:04 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Three postdoctoral positions are available beginning July 1 to study the molecular biology of cell-wall assembly of Neurospora crassa. These positions are funded from a 3-year NIH RO1. Requirements for two postions include experience with fungal growth and molecular biology of filamentous fungi, including screening libraries by sibselection, the isolation and cloning of genes and cDNAs. Some experience with Candida albicans a plus. The other postion will involve the purification of an integral membrane enzyme complex, (1,3)B-glucan synthase. Experience concerning protein purification a must. Please reply to: Claude P. Selitrennikoff Professor University of Colorado Health Sciences Center Denver CO 80262 I will be at the Fungal Genetics Meeting at Asilomar in late March. From owner-mycology@net.bio.net Tue Mar 07 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!ix.netcom.com!netcomsv!netcomsv!gatekeeper.genencor.com!usenet From: sdanko@genencor.com (Steve Danko) Newsgroups: bionet.mycology Subject: Re: Predatorial Fungi Date: 8 Mar 1995 00:05:14 GMT Organization: Genencor International, Inc. - (415) 742-7500 Lines: 28 Message-ID: <3jisbq$6id@gatekeeper.genencor.com> NNTP-Posting-Host: 10.1.1.41 X-Newsreader: AIR News 3.X (SPRY, Inc.) There exists a considerable volume of literature on the nematode-destroying fungi. Barron (Barron, G.L. 1977. The Nematode-Destroying Fungi. Topics in Mycology No. 1. 144 pp. Can. Biol. Publ. Ltd. Guelph, Ontario) divides these fungi in two groups: endoparasites and predators. The endoparasites produce spores that either become attached to the nematodes or are injested by the nematodes. Upon germination, the fungi then digest the worm. Example: *Harposporium anguillulae*. The predators produce constricting and nonconstricting hyphal rings and adhesive knobs or nets. Fungi such as *Dactylaria bronchopaga* and *Arthrobotrys dactyloides* are examples. You asked about soybean cyst nematode: *Heterodera glycines*. I don't know if any nematophagous fungi specifically attack this nemtode, but the American Type Culture Collection contains a fungus *Dactylella oviparisitica* isolated from egg masses of the root-knot nematode *Meloidogyne*. For further reading see Duddington, C.L. 1957. The Friendly Fungi. A New Approach to the Eelworm Problem. Faber and Faber, London. 188 pp. Hope this helps! Steve Danko Genencor International, Inc. sdanko@genencor.com From owner-mycology@net.bio.net Tue Mar 07 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!swrinde!pipex!warwick!leicester!usenet From: Anthony Hall Newsgroups: bionet.mycology Subject: wanted Verticillium agaricinum Date: 8 Mar 1995 16:35:08 GMT Organization: University of Leicester, UK Lines: 6 Message-ID: <3jkmbs$ft9@ipgate.le.ac.uk> NNTP-Posting-Host: pc6.botany.le.ac.uk Hi I'm still looking for a DNA prep or culture of Verticillium agaricinum Thankyou email ajwh1@leicester.ac.uk From owner-mycology@net.bio.net Wed Mar 08 22:00:00 1995 Path: biosci!CS.Arizona.EDU!math.arizona.edu!news.Arizona.EDU!ag!mccluske From: Kevin McCluskey Newsgroups: bionet.mycology Subject: Re: black pepper Date: Thu, 9 Mar 1995 10:15:22 -0700 Organization: The University of Arizona Lines: 18 Message-ID: References: <3jain5$o6@kisa.seanet.com> <3jcnl4$1ps@strauss.udel.edu> NNTP-Posting-Host: ag.arizona.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII In-Reply-To: In "On Food and Cooking" by Hal McGee (1984, Scribners) the production of black and white pepper is described. Black peppers are harvested green and fermented exposed to the sun. The black color on the surface is produced by the fungus Glomerella cingulata (the telomorph of Colletotrichum gloeosporioides). White pepper is harvested ripe (red), then fermented as black pepper, but before it is dried, the skin and some pulp is removed. Green and red pepper are available from some "gourmet" shops and catalogs. I expect that these are dried without fermenting, but the production of these varieties was not described in the book. From owner-mycology@net.bio.net Wed Mar 08 22:00:00 1995 Path: biosci!rutgers!gatech!swrinde!cs.utexas.edu!news.sprintlink.net!news.clark.net!kcowing-ppp.clark.net!user From: kcowing@aibs.org (Keith L. Cowing) Newsgroups: bionet.microbiology,sci.bio.microbiology,sci.bio.technology,bionet.cellbiol,bionet.mycology Subject: CALL FOR PROPOSALS: NASA NRA 95-01: Ground-Based and Small Payloads Research in Space Life Sciences Date: Thu, 09 Mar 1995 10:14:22 -0500 Organization: American Institute of Biological Sciences Lines: 173 Distribution: inet Message-ID: NNTP-Posting-Host: kcowing-ppp.clark.net Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit Xref: biosci bionet.microbiology:1646 sci.bio.microbiology:514 sci.bio.technology:2437 bionet.cellbiol:1774 bionet.mycology:1694 UPDATE: NASA NRA OLMSA 95-01 -- > Letters of Intent Due: March 24, 1995 <--- You can now download this entire NASA Research Announcement (NASA NRA 9501) from the AIBS Gopher (for Mac and PC) by selecting the "Professional Opportunities" folder and then looking within the "NASA" folder. You can connect to the AIBS gopher at: gopher.aibs.org or via WWW at: gopher://aibs.org +++++++++++++ NASA OMB Approval No. 2700-0042 NRA 95 OLMSA-01 NASA Research Announcement Soliciting Proposals for Ground-Based and Small Payloads Research in Space Life Sciences Life and Biomedical Sciences and Applications Division Office of Life and Microgravity Sciences and Applications National Aeronautics and Space Administration Date NRA Issued: January 26, 1995 -- > Letters of Intent Due: March 24, 1995 <--- Proposals Due: April 21, 1995 This National Aeronautics and Space Administration (NASA) Research Announcement (NRA) solicits proposals to participate in Ground-Based and Small Payloads Research in Space Life Sciences. This is a broad-based announcement that solicits research proposals for all of the major programs of the Life and Biomedical Sciences and Applications Division. The specific programs that are included in this announcement are Space Biology, Space Physiology and Countermeasures, Environmental Health, Space Radiation Health, Space Human Factors, Advanced Life Support, Advanced Extravehicular Activity Systems, Advanced Technology Development and Data Analysis. Proposals submitted in response to this Announcement may be either for standard ground-based research investigations or for small payload flight experiments or for both. Proposal to develop experiments aiming at space station utilization are particularly encouraged. Subsequent announcements similar to and based upon this Announcement will be updated and issued annually and will be the primary means of obtaining research proposals form the scientific community for ground and small payloads research in the space life sciences. Although this NRA is broad-based, it is restricted to the science programs named above and described in detail in Appendix A. The potential proposer should read the program descriptions that are of interest with care and attempt to focus the proposal on the specific research emphases defined in this Announcement. Participation in the Ground-based and Small Payloads Research in Space Life Sciences is open to all individuals and all categories of domestic and foreign organizations, industry, educational institutions, other nonprofit organizations, NASA laboratories, and other U.S. Governmental agencies. Proposals which will enhance or complement the scientific return from research currently being supported by the National Institutes of Health, National Science Foundation, or other Government agencies are encouraged. In addition, proposals to advance technology and develop practical applications of technology are sought under this Announcement. Because of limitations of access to flight opportunities, it is expected that the majority of proposals approved in response to this Announcement would be for ground-based research. A letter of Intent to Propose is requested by March 24, 1995. Proposals may be submitted at any time up to April 21, 1995. Proposals will be evaluated for scientific/technical value, soundness, intrinsic scientific/technical value, relevance, implementation feasibility, and cost. A selection announcement will be made between August and October of 1995. Funding of selected proposals will begin sometime between October 1995 and September 1996. Proposals due: April 21, 1995 Letters of Intent Due: March 24, 1995 Number of copies Requested: 20 NASA Selecting official: Dr. Joan Vernikos Director, Life and Biomedical Sciences and Applications Division/ Code ULR NASA Headquarters Washington, DC 20564-0001 Obtain additional information from the appropriate Science Program manager, as indicated below, at the following address: UL/ Life and Biomedical Sciences and Applications Division NASA Headquarters Washington, DC 20546-0001 Telephone: 202-358-2530 Fax: 202-358-4168 Program: Program Manager/Email Space Biology: Dr. Mary Anne Frey MAFrey@smtpgmgw.olmsa.hq.nasa.gov Space Physiology and Countermeasures: Dr. Victor S. Schneider vschneider@smtpgmgw.olmsa.hq.nasa.gov Environmental Health: Dr. Victor S. Schneider vschneider@smtpgmgw.olmsa.hq.nasa.gov Space Radiation Health: Dr. Frank M. Sulzman fsulzman@smtpgmgw.olmsa.hq.nasa.gov Space Human Factors: Dr. Guy C. Fogleman GCFogleman@smtpgmgw.olmsa.hq.nasa.gov Advanced Life Support: Dr. Maurice M. Averner maverner@smtpgmgw.olmsa.hq.nasa.gov Advanced Extravehicular Activity Systems: Mr. Robert K. Callaway RCallaway@smtpgmgw.olmsa.hq.nasa.gov Advanced Technology Development: Mr. Gregory K. Schmidt gschmidt@smtpgmgw.olmsa.hq.nasa.gov Data Analysis: Dr. Ronald J. White RJWhite@smtpgmgw.olmsa.hq.nasa.gov Small Payloads Program: Mr. Marc Shepanek mshepanek@smtpgmgw.olmsa.hq.nasa.gov Proposals and Letters of Intent mailed through the U.S. Postal Service by express, first class, registered, or certified mail are to be sent to the following address: Ronald J. White, PhD. Life and Biomedical Sciences and Applications Division/ Code UL NASA Headquarters Washington, DC 20546-0001 Proposals and Letters of Intent hand delivered or sent by commercial delivery or courier services are to be delivered between the hours of 8 AM and 4:30 PM: Ronald J. White, PhD. Life and Biomedical Sciences and Applications Division/ Code UL National Aeronautics and Space Administration ATTN: Receiving and Inspection (Rear of Building) 300 E street, SW Washington, DC 20024-3210 The telephone number 202-488-2940 may be used when required for reference by delivery services. NASA cannot receive deliveries on Saturdays, Sundays, or federal Holidays. Special instructions apply to foreign (non-U.S.) proposals. From owner-mycology@net.bio.net Wed Mar 08 22:00:00 1995 Path: biosci!rutgers!gatech!newsxfer.itd.umich.edu!caen!night.primate.wisc.edu!news.doit.wisc.edu!F180-114.net.wisc.edu!jmicales From: jmicales@facstaff.wisc.edu (Jessie Micales) Newsgroups: bionet.mycology Subject: Scalp mycosis??? Date: Thu, 9 Mar 1995 08:24:10 Organization: U.S. Forest Service Lines: 11 Message-ID: NNTP-Posting-Host: f180-114.net.wisc.edu Any medical mycologists out there? Our secretary noticed yesterday that she has two circular areas on her scalp in which all the hair is gone. The patches are circular, very red and irritated looking (although they don't hurt), and about 2 cm in diameter. She went to the doctor (a general practioner) who had never seen anything like it before. He took some samples and will presumably do some culturing. Any suggestions in the interim? She, of course, is quite worried. Jessie (jmicales@facstaff.wisc.edu) From owner-mycology@net.bio.net Wed Mar 08 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!newsjunkie.ans.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: mgkmkr@aol.com (MGKMKR) Newsgroups: bionet.mycology Subject: A. Vellaneous Date: 8 Mar 1995 19:44:39 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 1 Sender: root@newsbf02.news.aol.com Message-ID: <3jlj1n$pav@newsbf02.news.aol.com> Reply-To: mgkmkr@aol.com (MGKMKR) NNTP-Posting-Host: newsbf02.mail.aol.com Can anyone identify this term? From owner-mycology@net.bio.net Thu Mar 09 22:00:00 1995 Path: biosci!ESSEX.HSC.COLORADO.EDU!claudes From: claudes@ESSEX.HSC.COLORADO.EDU (Claude Selitrennikoff) Newsgroups: bionet.mycology Subject: postdoc positions Date: 10 Mar 1995 06:33:59 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 25 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Three postdoctoral positions are available beginning July 1 to study the molecular biology of cell-wall assembly of Neurospora crassa. These positions are funded from a 3-year NIH RO1. Requirements for two postions include experience with fungal growth and molecular biology of filamentous fungi, including screening libraries by sibselection, the isolation and cloning of genes and cDNAs. Some experience with Candida albicans a plus. The other postion will involve the purification of an integral membrane enzyme complex, (1,3)B-glucan synthase. Experience concerning protein purification a must. Please reply to: Claude P. Selitrennikoff Professor University of Colorado Health Sciences Center Denver CO 80262 I will be at the Fungal Genetics Meeting at Asilomar in late March. From owner-mycology@net.bio.net Thu Mar 09 22:00:00 1995 Path: biosci!utmb.edu!chester.cooper From: chester.cooper@utmb.edu (Chester Cooper) Newsgroups: bionet.mycology Subject: Re: Important Opportunity from J Mugg Date: 10 Mar 1995 06:04:35 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HNYPBRM4SI0063I1@BEACH.UTMB.EDU> NNTP-Posting-Host: net.bio.net To all bionetters: To me, this so-called great opportunity recently posted from Ms. Mugg and Di Cesare that purportedly has no similarity to a pyramid scheme (as my son so often says, "Yea, Dad,and monkeys fly out of my butt!") does have a profound resemblance to a typical New York street "Mugg"-ing. Should we render unto "DiCesare"? Is anyone else out there tired of this kind of crap posted to our science net groups? From owner-mycology@net.bio.net Thu Mar 09 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!cs.utexas.edu!news.sprintlink.net!pipex!lyra.csx.cam.ac.uk!warwick!news.shef.ac.uk!silver!mb1jxk From: mb1jxk@silver.shef.ac.uk (J Kennedy) Newsgroups: bionet.mycology Subject: GTG start for fungal genes? Date: 10 Mar 1995 09:51:42 GMT Organization: University of Sheffield, UK Lines: 1 Message-ID: <3jp7fe$2pj@hippo.shef.ac.uk> NNTP-Posting-Host: silver.shef.ac.uk X-Newsreader: TIN [version 1.2 PL2] From owner-mycology@net.bio.net Thu Mar 09 22:00:00 1995 Path: biosci!ESSEX.HSC.COLORADO.EDU!claudes From: claudes@ESSEX.HSC.COLORADO.EDU (Claude Selitrennikoff) Newsgroups: bionet.mycology Subject: Re: Important Opportunity from J Mugg Date: 10 Mar 1995 09:32:10 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 5 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <01HNYPBRM4SI0063I1@BEACH.UTMB.EDU> NNTP-Posting-Host: net.bio.net I couldn't agree more: this certainly does not belong in a science group!!! Claude Selitrennikoff University of Colorado From owner-mycology@net.bio.net Thu Mar 09 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!swrinde!sgiblab!uhog.mit.edu!news.mtholyoke.edu!news.umass.edu!nic.umass.edu!k12.oit.umass.edu!puchalsk From: puchalsk@k12.oit.umass.edu (Joshua Puchalski (GCC/TEME)) Newsgroups: bionet.mycology Subject: potato dextrose broth? Date: 10 Mar 1995 15:25:45 GMT Organization: University of Massachusetts K-12 Outreach Lines: 12 Message-ID: <3jpr1p$cso@nic.umass.edu> NNTP-Posting-Host: k12.ucs.umass.edu X-Newsreader: TIN [version 1.2 PL2] I know several places sell a potato dextros agar mixture, but does is there a ready made (i.e. just add water) broth? The same mixture, just without the agar. If so, where could I order it from? Thanks a lot puchalsk@k12.oit.umass.edu Joshua Puchalski From owner-mycology@net.bio.net Thu Mar 09 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.cac.psu.edu!news.tc.cornell.edu!newsserver.sdsc.edu!nic-nac.CSU.net!charnel.ecst.csuchico.edu!csusac!csus.edu!news.ucdavis.edu!dale!fzejones From: fzejones@dale.ucdavis.edu (Elizabeth Jones) Newsgroups: bionet.mycology Subject: powdery mildews Date: 9 Mar 1995 22:30:10 GMT Organization: University of California, Davis Lines: 12 Message-ID: <3jnvhi$mv6@mark.ucdavis.edu> NNTP-Posting-Host: dale.ucdavis.edu Keywords: powdery mildews, tomato, Leveillula taurica X-Newsreader: TIN [version 1.2 PL2] Hi, I would like to make contact with anyone working on powdery mildews, particularly on tomato, and particularly Leveillula taurica. I am trying to study molecular variability in Californian L. taurica isolates and would like to obtain freeze dried/DNA samples of isolates of L. taurica and other tomato powdery mildews (Oidium or Erysiphe) from around the world to use as comparisons/controls. If you work on any of these pathogens, or know someone who does, please let me know. Also, I have had considerable difficulties maintaining L. taurica in an invitro system (on tomato plantlets in magenta boxes) and in getting decent DNA of L. taurica. The principal problem seems to be getting a very tough spore wall open. Any ideas? Know of any good papers? Thanks, Elizabeth Jones From owner-mycology@net.bio.net Fri Mar 10 22:00:00 1995 Path: biosci!adam.cc.sunysb.edu!news.nysernet.net!news.sprintlink.net!psgrain!news.teleport.com!usenet From: "Ralph D. Arnold" Newsgroups: bionet.mycology Subject: Will You Help Answer These, Please? Date: Fri, 10 Mar 95 21:24:55 PST Organization: Teleport - Portland's Public Access (503) 220-1016 Lines: 100 Message-ID: <86857.rarnold@teleport.com> Reply-To: NNTP-Posting-Host: ip-pdx2-17.teleport.com X-Minuet-Version: Minuet1.0_Beta_17A X-POPMail-Charset: English As moderator of FUNGUS, the almost-daily email newsletter on mushroom cultivation, I have posted these questions, which unfortunately FUNGUS readers haven't been able/willing to answer. I'm appealing to you bionet.mycology readers for help. (I'll post any answers to an upcoming FUNGUS emailing). Thanks! Ralph Arnold (rarnold@teleport.com) 1. Reed Retort Seal Problems? 2. Contamination of Spawn? 3. Mushrooms in Caves? 4. Marketing Advise in Boston? //////////////////////////////////////////////////////////////////////// Date: Wed, 8 Mar 1995 17:08:05 -0800 From: PEHLING@coopext.cahe.wsu.edu (Dave Pehling) Subject: Reed Retort seal problems?? Hi Fungus Folk, One of our mushroom-growers in the County is having trouble with the seal sticking on her Reed retort. Her instructions say that she should use a mix of water and graphite on the seal to prevent this. She has not been able to find a source for this material. Any suggestions? Thanks in advance for your help! Cheers, Dave Pehling ----------------------------------------------------------------------- W.S.U. Cooperative Extension/Snohomish County 600 - 128th St. SE Everett, WA. 98208 U.S.A. phone - (206) 338-2400 FAX - (206) 338-3994 email - PEHLING@coopext.cahe.wsu.edu ///////////////////////////////////////////////////////////////////// From: "Buzz Bell" Date: Tue, 7 Mar 1995 16:01:21 +0500EST Subject: "Contamination of Spawn?" I have a bright red contaminant in some of my spawn(millet). I frequently(weekly) use a dilute bleach solution on all of my work surfaces to disinfect them. I soak all of my instruments in 100% IsOH until use, and flame them repeatedly when transferring, cloning, etc. Contaminated spawn is immediately discarded, although it is a recurring problem. Also, a blue-green contaminant which looks similar to the ends of a fruticose lichen grows in my compost, but I scoop this out with a flamed instrument. Does anyone know what either of these contaminants are? One more question. When my compost is run through fully, or at least waiting a few more days shows very minimal additional growth, I applied casing soil(sphagnum peat, ph neuralized with lime). It has been almost two weeks, and I do not see any pin-heads appearing yet. I do see some mycelia coming up through the casing soil. Any ideas on what's going on? ///////////////////////////////////////////////////////////////////// From: leucim@ext.missouri.edu Date: Mon, 06 Mar 95 13:28:57 cdt Subject: mushrooms in caves? Hello, I have a client who is interested in information about growing mushrooms in caves. He works at Meramec State Park (Missouri) where there are a lot of caves. Supposedly around the turn of the century there was a gentleman who was growing mushrooms in one of the caves and selling the mushrooms to local restaurants. What types of mushrooms can be grown successfully in caves? Are there presently any commercial scale cave mushroom growers in the US? Where can someone find information on this specific topic? Thanks for your help. Debi Kelly Missouri Alternatives Center 628 Clark Hall Columbia, MO 65211 314-882-1905 ///////////////////////////////////////////////////////////////////// Date: Fri, 3 Mar 95 08:15:51 EST From: okerholm_pau@us4rmc.pko.dec.com Subject: Marketing Advise in Boston??? Hi, I'm back with more questions. I have quite an E-Mail dialog going with a grower from the West Coast so the net is working well for me. I had posted a question in another newsgroup and Ralph thought it might apply here as well. So I rephrased it a bit and here it is. I am hoping to sell Shiitake mushrooms to the food service market in the Boston area. Boston has a large produce market but I have no idea how to connect with it. Do you have to belong to a co-op or other organization? Do you have to deal through a broker? If so how do you make contact with them? My questions are about the East but I assume they are generic and would apply to any major metropolitan market. If anyone has experience in selling to a large metropolitan market, I'd appreciate a reply. Thanks, Paul ///////////////////////////////////////////////////////////////////// From owner-mycology@net.bio.net Fri Mar 10 22:00:00 1995 Path: biosci!agate!news.mindlink.net!news.bc.net!torn!ccshst05.cs.uoguelph.ca!news From: Ken Ursic Newsgroups: bionet.mycology Subject: Root Biology and Mycorrhiza Research Group WWW PAGE Date: 10 Mar 1995 20:24:14 GMT Organization: University of Guelph Lines: 6 Message-ID: <3jqche$bkj@ccshst05.cs.uoguelph.ca> NNTP-Posting-Host: ccshst01.cs.uoguelph.ca Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 7bit The root biology and Mychorrhiza research group at the University of Guelph has an experimental WWW page up. It is still under construction so check it out periodically. the URL is http://www.uoguelph.ca/CBS/roots.htm Ken Ursic From owner-mycology@net.bio.net Fri Mar 10 22:00:00 1995 Path: biosci!agate!spool.mu.edu!uwm.edu!news.alpha.net!solaris.cc.vt.edu!insosf1.infonet.net!newshost.marcam.com!charnel.ecst.csuchico.edu!psgrain!news.teleport.com!news.teleport.com!not-for-mail From: larryc@teleport.com (Larry Caldwell) Newsgroups: bionet.mycology Subject: Re: MOReLs Date: 11 Mar 1995 02:09:55 -0800 Organization: Teleport - Portland's Public Access (503) 220-1016 Lines: 27 Message-ID: <3jrstj$815@kelly.teleport.com> NNTP-Posting-Host: kelly.teleport.com rwinder@PFC.Forestry.CA (Richard Winder) writes: |:Yes! If anyone could report the northward progress of morel fruiting in |:North America &/or the West Coast, it would really be a big help to those of |:us who have to plan dates for mushroom club forays (some members in my |:group are still not convinced there's even such thing as a morel- we seem |:to have hit all the wrong dates and places last year.) Morel hunting is not an armchair occupation. I doubt you will have much luck, considering the variety of microclimates on the Pacific coast. I spotted a couple verpa bohemica last week around Roseburg. There weren't enough for a meal, and I was planting a thousand Douglas Fir seedlings, so I covered a *lot* of ground. The weather was warm and sunny. 200 miles farther south it was cold and rainy. A few days of local fog can screw up a morel fruiting. The best thing you can do is wait until the ground starts to warm and then go out and hike 20 miles with your nose at knee level. Remember to outfit your club members with a survival pack. .. Kissing an ashtray is like licking a smoker. -- MR/2 #109 -- Larry Caldwell larryc@teleport.com From owner-mycology@net.bio.net Fri Mar 10 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!uunet!in1.uu.net!spool.mu.edu!torn!ccshst05.cs.uoguelph.ca!ccshst01.cs.uoguelph.ca!sannis From: sannis@uoguelph.ca (Seanna Annis) Newsgroups: bionet.mycology Subject: Re: Need meth. to distinguish endo/exo glucanase Date: 10 Mar 1995 22:46:11 GMT Organization: University of Guelph Lines: 22 Message-ID: <3jqkrj$fvi@ccshst05.cs.uoguelph.ca> References: <3iva0k$qur@sifon.cc.mcgill.ca> NNTP-Posting-Host: ccshst01.cs.uoguelph.ca X-Newsreader: TIN [version 1.2 PL2] Christian Archambault (popa0215@PO-Box.McGill.CA) wrote: : I would need a method to determine if the protein we purified is an endo or exo beta-1,3 glucanase. Any suggestion ??? : Christian Archambault : popa0215@po-box.mcgill.ca You can easily do this by viscosity. There are a lot of papers on using the technique for determining endo and exo polygalacturonases and this should work on glucanases depending upon what type of glucanase you have. If carboxy methyl cellulose is a substrate for your enzyme you can make a viscous solution add your enzyme and then measure the viscosity at set intervals after you added your enzyme then plot determine the change in viscosity for each time and plot this against the reducing sugar released by your enzyme at each time (ie an identical time course except determine the reducing sugars released) If the released reducing sugars are very high with a 50 % change in the viscosity than you have an exo enzyme otherwise if there are low levels of reducing sugar you have a endo enzyme. If you need more info I can provide it otherwise" Hoffman and Turner, 1982 physiological plant pathology" is a paper describing the viscosity method among other methods for polygalacturonase activity determination. You can use the same methods on a variety of degradative enzymes. Good luck Seanna Annis. sannis@uoguelph.ca From owner-mycology@net.bio.net Fri Mar 10 22:00:00 1995 Path: biosci!rbge.org.uk!GORDON From: GORDON@rbge.org.uk ("GORDON RUTTER") Newsgroups: bionet.mycology Subject: subscribe Date: 11 Mar 1995 13:13:42 -0800 Organization: Royal Botanic Garden Edinburgh Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <84AE2A4698@rbg-3.rbge.org.uk> NNTP-Posting-Host: net.bio.net subscribe From owner-mycology@net.bio.net Fri Mar 10 22:00:00 1995 Path: biosci!agate!newsxfer.itd.umich.edu!gatech!swrinde!howland.reston.ans.net!news.moneng.mei.com!uwm.edu!spool.mu.edu!torn!ccshst05.cs.uoguelph.ca!ccshst01.cs.uoguelph.ca!kursic From: kursic@uoguelph.ca (Ken A Ursic) Newsgroups: bionet.mycology Subject: Re: Root Biology and Mycorrhiza Research Group WWW PAGE Date: 11 Mar 1995 21:52:49 GMT Organization: University of Guelph Lines: 9 Message-ID: <3jt63h$2jn@ccshst05.cs.uoguelph.ca> NNTP-Posting-Host: ccshst01.cs.uoguelph.ca X-Newsreader: TIN [version 1.2 PL2] The Proper URL for the Root Biology and Mycorrhiza Group is http://www.uoguelph.ca/CBS/Botany/roots.htm -- Ken Ursic Cliff Ecology Research Group Dept. of Botany, University of Guelph Guelph, Ontario, CANADA http://www.uoguelph.ca/CBS/Botany/index.htm From owner-mycology@net.bio.net Fri Mar 10 22:00:00 1995 Path: biosci!agate!newsxfer.itd.umich.edu!gatech!swrinde!howland.reston.ans.net!spool.mu.edu!torn!ccshst05.cs.uoguelph.ca!ccshst01.cs.uoguelph.ca!kursic From: kursic@uoguelph.ca (Ken A Ursic) Newsgroups: bionet.mycology Subject: Re: Root Biology and Mycorrhiza Research Group WWW PAGE Date: 11 Mar 1995 21:50:35 GMT Organization: University of Guelph Lines: 15 Message-ID: <3jt5vb$2jn@ccshst05.cs.uoguelph.ca> References: <3jqche$bkj@ccshst05.cs.uoguelph.ca> NNTP-Posting-Host: ccshst01.cs.uoguelph.ca X-Newsreader: TIN [version 1.2 PL2] Ken Ursic (kursic@uoguelph.ca) wrote: : The root biology and Mychorrhiza research group at the University : of Guelph has an experimental WWW page up. It is still under : construction so check it out periodically. : the URL is http://www.uoguelph.ca/CBS/roots.htm : Ken Ursic SORRY THE URL REALLY IS http://www.uoguelph.ca/CBS/Botany/roots.htm -- Ken Ursic Cliff Ecology Research Group Dept. of Botany, University of Guelph Guelph, Ontario, CANADA http://www.uoguelph.ca/CBS/Botany/index.htm From owner-mycology@net.bio.net Fri Mar 10 22:00:00 1995 Path: biosci!CLS.BIOL.SCAROLINA.EDU!CZAKO From: CZAKO@CLS.BIOL.SCAROLINA.EDU Newsgroups: bionet.mycology Subject: Hypoxylon fragiforme Date: 11 Mar 1995 10:15:21 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I would like to obtain a sample of Hypoxylon fragiforme or another green Hypoxylon species. I have read a paper on a green fungal pigment, hypoxyxylerone with great interest (Edwards RL, Fawcett V, Maitland DJ, Nettleton R, Shields L, Whalley JS: Hypoxyxylerone. A novel green pigment from the fungus Hypoxylon fragiforme (Pers.: Fries) Kickx. J Chem Soc, Chem Commun 1009-10, 1991). Although, principally I am interested in the biosynthesis of complex plant pigments of heartwood, I like to learn about novel chromophores of other kingdoms. Especially if it is from a fungus that may grow in wood. Can these fungi cause green stains in woods? Basically, I try to find out if the pigment I am dealing with is of plant or maybe Hypoxylon origin. I know it is not due to Chlorociboria sp. I would greatly appreciate if one could send me a Hypoxylon fragiforme isolate that is green, or a similar green species. Dr. Mihaly Czako Research Associate Department of Biological Sciences University of South Carolina 700 Sumter st. Columbia, SC 29208 Tel.: 803-777-8928 FAX: 803-777-4002 Czako@biol.scarolina.edu From owner-mycology@net.bio.net Sat Mar 11 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!newsjunkie.ans.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: randbio@aol.com (RandBio) Newsgroups: bionet.mycology Subject: Re: potato dextrose broth? Date: 12 Mar 1995 17:46:49 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 6 Sender: root@newsbf02.news.aol.com Message-ID: <3jvtkp$q4t@newsbf02.news.aol.com> References: <3jpr1p$cso@nic.umass.edu> Reply-To: randbio@aol.com (RandBio) NNTP-Posting-Host: newsbf02.mail.aol.com Randolph Biomedical is a speciality culture media manufacturer. They prepare small lots of Hard To Find preparation or media formulas referenced in reprints but not commercially available. They also will make custom formulations. They can be reached at 401-826-1407 (USA) or e-mail randbio@aol.com This is not intended to be a commerical use of this newsgroup - but an effort to let you know of a source. RR From owner-mycology@net.bio.net Sat Mar 11 22:00:00 1995 Path: biosci!agate!news.duke.edu!news-feed-1.peachnet.edu!gatech!howland.reston.ans.net!ix.netcom.com!netcomsv!uucp3.netcom.com!hood.paros.com!metro.atlanta.com!pop.atlanta.com!dakraut From: dakraut@atlanta.com (David Kraut) Newsgroups: bionet.mycology Subject: Mycolog Date: Sun, 12 Mar 1995 08:43:39 Organization: Internet Atlanta Lines: 8 Message-ID: NNTP-Posting-Host: dakraut.atlanta.com X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Maybe I am out of line here but I was hoping to find out if anyone knows of a product called Mycolog. My main interest is the long term effects of using this product. I have been forced to continually use this product to subdue the effects of what several doctors have told me is an allergy. I have had a red rash that developed at the corner of my lips and Mycolog is the only thing that will minimize this embarrasing problem. However I am worried that my liver may be taking a long term beating. Thanks in advance, DAVE. dakraut@atlanta.com From owner-mycology@net.bio.net Sun Mar 12 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!agate!library.ucla.edu!csulb.edu!paris.ics.uci.edu!news.service.uci.edu!gandalf.bio.uci.edu!rdlab From: rdlab@gandalf.bio.uci.edu (rlab davis) Newsgroups: bionet.mycology Subject: Neurospora RNA for RNase protection Date: Sun, 12 Mar 1995 19:14:03 Organization: Bio Sci Lines: 12 Message-ID: NNTP-Posting-Host: davislab3.bio.uci.edu X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Is anyone using a particular RNA isolation protocol for Neurospora which yields total RNA suitable for RNase protection? I seem to be having trouble with DNA contamination using techniques published in the FGN. Ideally, I would like a technique which is quick, easy and doesn't involve isolating mRNA from total. Is this wishful thinking? If you have any suggestions, please email me. Thanks in advance. Martin Hoyt Dept. of Molecular Biology and Biochemistry, Univ. Calif. Irvine mahoyt@uci.edu From owner-mycology@net.bio.net Sun Mar 12 22:00:00 1995 Path: biosci!LABA.TDH.TEXAS.GOV!JHARRIS From: JHARRIS@LABA.TDH.TEXAS.GOV ("Jim Harris") Newsgroups: bionet.mycology Subject: Workshops Date: 13 Mar 1995 09:42:14 -0800 Organization: Texas Department of Health LABs Lines: 34 Sender: daemon@net.bio.net Distribution: world Message-ID: <34D00A037DC@laba.tdh.texas.gov> NNTP-Posting-Host: net.bio.net Because there have been so many persons adding to the mycology group and because my e-mail address has changed this week, I am resubmitting the following workshop information for interested mycologists. If you have responded by e-mail prior to last week, I am confident that I received your inquiry and you should have received the printed information from me by now. Workshop #1 Fungi to Be Feared? Condo Contaminants and Toxic Toadstools Scheduled for May 18-19, 1995 in Washington, D.C. Presenters: L. Sigler, R. Summerbell, B. Jarvis, D. Miller, R. Gots, S. Epstein. Fee: Before April 15 is $200, after April 15, $250. Workshop #2 Identification of Significant Species of Aspergillus and Penicillium Scheduled for July 31-August 4, 1995 in San Antonio, TX Presenters: J. Pitt, M. Klich Fee: Before July 4 is $695, after July 4, $750 For more information on either of these programs, please send me your mailing address or a FAX number. Jim Harris, Ph.D. Training Coordinator Bureau of Laboratories Texas Department of Health 1100 West 49th Street Austin, TX 78756 jharris@laba.tdh.state.tx.us From owner-mycology@net.bio.net Sun Mar 12 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!EU.net!uknet!bcc.ac.uk!is.bbsrc.ac.uk!news From: firstname.surname@bbsrc.ac.uk (windy) Newsgroups: bionet.mycology Subject: Peronospora parasitica Date: 13 Mar 1995 15:45:02 GMT Organization: Institute of Arable Crops Research, Rothamsted Lines: 12 Message-ID: <3k1p9u$ron@is.bbsrc.ac.uk> Reply-To: herana@bbsrc.ac.uk NNTP-Posting-Host: pc838.res.afrc.ac.uk X-Newsreader: WinVN 0.92.6+ I'm looking for an isolate of P. parasitica that can infect Brassica juncea *cv. Kranti*, an Indian cultivar. To date I have screened a number of Indian and English isolates. If anybody has an isolate that can be regenerated on cv. Kranti, I would be very pleased to hear from you. Please email me if possible, as I do not regularly read this newsgroup. happiness...... Windy Heran. From owner-mycology@net.bio.net Sun Mar 12 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!news.sprintlink.net!howland.reston.ans.net!spool.mu.edu!uwm.edu!msunews!harbinger.cc.monash.edu.au!news.cs.su.oz.au!metro!unsw.edu.au!aix00.csd.unsw.OZ.AU!p8443882 From: p8443882@aix00.csd.unsw.OZ.AU (David Orlovich) Newsgroups: bionet.mycology Subject: Re: Scalp mycosis??? Date: 13 Mar 1995 00:59:44 GMT Organization: University of New South Wales, Sydney Australia. Lines: 2 Message-ID: <3k05e0$cjf@mirv.unsw.edu.au> References: NNTP-Posting-Host: aix00.csd.unsw.oz.au Sounds like ringworm ... Tinea capitis From owner-mycology@net.bio.net Sun Mar 12 22:00:00 1995 Path: biosci!forestry.utoronto.ca!dobranic From: dobranic@forestry.utoronto.ca (Jason Dobranic) Newsgroups: bionet.mycology Subject: Metarhizium Studies Date: 13 Mar 1995 07:53:00 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 4 Sender: daemon@net.bio.net Distribution: world Message-ID: <9503131559.AA14882@forestry.utoronto.ca> NNTP-Posting-Host: net.bio.net Hi all, I am interested in making contact with other researchers that are working on Metarhiziun anisopliae as a biocontrol agent. For exchange of ideas and procedures. Hope to hear from you soon! From owner-mycology@net.bio.net Sun Mar 12 22:00:00 1995 Path: biosci!agate!newsxfer.itd.umich.edu!gatech!howland.reston.ans.net!news.cac.psu.edu!psuvm!hgw1 Organization: Penn State University Date: Mon, 13 Mar 1995 09:57:21 EST From: Heather Treaster Message-ID: <95072.095721HGW1@psuvm.psu.edu> Newsgroups: bionet.mycology Subject: Grad. Assistantships Available Lines: 15 Graduate Assistantships Available. The Penn State Plant Pathology Department has several assistantships available for M.S. or Ph.D. students. Faculty programs include a diverse range of research in basic and applied plant pathology, including: field and greenhouse crop pathology, forest pathology, sustainable agriculture, molecular fungal taxonomy, mushroom culture and pathology, fungal genetics, epidemiology, virology, plant responses to environmental stress, air pollution effects to forest trees and ecosystems, root-specific metabolism, root-pathogen interactions, host-parasite interactions. Excellent facilities available for field work and biochemical/molecular biology research. Contact Dr. J.E. Ayers, Graduate Admissions Committee, Plant Pathology Department, 211 Buckhout Lab., University Park, PA 16802 (Phone: 814-865-7069; Fax: 814-863-7217; E-mail: htreaste@psupen.edu). Affirmative Action/Equal Opportunity Employer; womenand minorities encouraged to apply. From owner-mycology@net.bio.net Sun Mar 12 22:00:00 1995 Path: biosci!PLANTPATH.WISC.EDU!AHE From: AHE@PLANTPATH.WISC.EDU ("Albert H Ellingboe") Newsgroups: bionet.mycology Subject: unsubcribe Date: 13 Mar 1995 15:41:35 -0800 Organization: University of Wisconsin Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <197C1C15599@charon.plantpath.wisc.edu> Reply-To: ahe@plantpath.wisc.edu NNTP-Posting-Host: net.bio.net unsubscribe. I will be away from my office for two months. I will wish to subscribe again when I reyurn. ************************************** Albert H. Ellingboe Room 593 Department of Plant Pathology 1630 Linden Drive Madison, WI 53706-1598 (608) 262-7269 FAX (608) 263-2626 -------------------------------------- From owner-mycology@net.bio.net Sun Mar 12 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!uunet!in1.uu.net!nntp.cac.washington.edu!homer10.u.washington.edu!todell From: Thomas O'Dell Newsgroups: bionet.mycology Subject: Morels Date: Mon, 13 Mar 1995 12:51:35 -0800 Organization: University of Washington Lines: 6 Message-ID: NNTP-Posting-Host: homer10.u.washington.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII I found one today on the Universitry of Washington campus, first I've heard of this spring in the puget sound region. Anyone else? todell@u.washington.edu From owner-mycology@net.bio.net Sun Mar 12 22:00:00 1995 Path: biosci!ADMIN.OGI.EDU!msachs From: msachs@ADMIN.OGI.EDU (Matthew Sachs) Newsgroups: bionet.mycology Subject: Re: Neurospora RNA for RNase protection Date: 13 Mar 1995 12:45:29 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 45 Sender: daemon@net.bio.net Distribution: world Message-ID: <9503132040.AA09934@admin.ogi.edu> NNTP-Posting-Host: net.bio.net >Is anyone using a particular RNA isolation protocol for Neurospora which >yields total RNA suitable for RNase protection? I seem to be having trouble >with DNA contamination using techniques published in the FGN. > >Ideally, I would like a technique which is quick, easy and doesn't involve >isolating mRNA from total. Is this wishful thinking? > >If you have any suggestions, please email me. Thanks in advance. > >Martin Hoyt >Dept. of Molecular Biology and Biochemistry, Univ. Calif. Irvine >mahoyt@uci.edu 1. Lower pH (5-6) in aqueous buffers for RNA extraction is reported to reduce the recovery of contaminating DNA. 2. A simple but not quantitative method to separate DNA (and small RNA) from larger RNA, such as mRNA, is on the basis of their differential solubilities in lithium chloride. 3. RNase-free DNase can be used to treat total RNA samples. See: Wallace, D. M. (1987). Large- and small-scale phenol extractions. Meth. Enzymol. 152, 33-41. Wallace, D. M. (1987). Precipitation of nucleic acids. Meth. Enzymol. 152, 41-48. ----------------------------------------------------------- Matthew Sachs Department of Chemistry, Biochemistry and Molecular Biology Oregon Graduate Institute of Science and Technology 20000 NW Walker Road P.O. Box 91000 Portland, OR 97291-1000 503 690-1487 Phone 503 690-1464 Fax msachs@admin.ogi.edu From owner-mycology@net.bio.net Sun Mar 12 22:00:00 1995 Path: biosci!MANI.CBS.UMN.EDU!npv From: npv@MANI.CBS.UMN.EDU (Nora Plesofsky-Vig) Newsgroups: bionet.mycology Subject: re: RNA from Neurospora Date: 13 Mar 1995 10:14:17 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 41 Sender: daemon@net.bio.net Distribution: world Message-ID: <9503131810.AA00730@mani.cbs.umn.edu> Reply-To: nora@biosci.cbs.umn.edu NNTP-Posting-Host: net.bio.net To Martin Hoyt: We have developed a procedure for isolating RNA from Neurospora that is quick and appears clean, but we have not tested the RNA in protection studies: RNA Extraction Buffer 8 M Guanidine HCl (practical grade) 50 mM Tris, pH 7.5 1 mM EDTA 8% v/v 2-mercaptoethanol 0.5% N-lauroylsarcosine filter sterilize (0.45 uM filter), store 4oC 1. Homogenize c. 200 mg cells for 60 sec (we use MSK, with cooling and 2.7 g glass beads for homogenization) in either one or two ml (larger vol throughout for spores) of RNA extraction buffer. 2. Put homogenate in Eppendorf tubes; wash beads with 200 or 400 ul of extraction buffer and add to tube(s). Leave tubes at room temp for 5 min. 3. Spin in microfuge (15,000 x g) for 10 min at 4oC. Collect supernatant and spin again. 4. To second supernatant (600-800 ul), add 1/3 vol of ddwater and 1/3 vol of 10 M LiCl. The final concentration should be 2 M LiCl and 4.8 M Guanidine HCl. 5. Ppt. RNA overnight at 4oC. 6. To recover ppt RNA, spin in microfuge at 4oC for 10 min, wash pellet 3x with 300 ul of 2 M LiCl and 3x with 300 ul of (-20oC) 95% ethanol 7. Dry pellet in speed vac and dissolve in DEPC-treated ddwater (50-100 ul). RNA pellets dissolve within 15 min at room temp. Clean RNA of insoluble material by brief spin, transferring supernatant to clean tube. Store RNA at -80oC. Nora Plesofsky-Vig From owner-mycology@net.bio.net Mon Mar 13 22:00:00 1995 Path: biosci!rutgers!gatech!swrinde!howland.reston.ans.net!nntp.crl.com!crl2.crl.com!not-for-mail From: labware@crl.com (John Brookes) Newsgroups: bionet.mycology Subject: For Sale: Discount Lab Glassware Date: 13 Mar 1995 17:57:39 -0800 Organization: CRL Dialup Internet Access Lines: 9 Message-ID: <3k2t6j$1ot@crl2.crl.com> NNTP-Posting-Host: crl2.crl.com X-Newsreader: TIN [version 1.2 PL2] CyberGlass is offering Laboratory Glassware of all types for sale at discount prices: New............................30% off list price Used (like new condition)......40% off list price Please e-mail us for details! John Brookes From owner-mycology@net.bio.net Mon Mar 13 22:00:00 1995 Path: biosci!rutgers!gatech!swrinde!cs.utexas.edu!news.sprintlink.net!uunet!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: chilicatt@aol.com (Chilicatt) Newsgroups: bionet.mycology Subject: Photos for new diag micro text Date: 13 Mar 1995 21:19:25 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 10 Sender: root@newsbf02.news.aol.com Message-ID: <3k2ufd$g19@newsbf02.news.aol.com> Reply-To: chilicatt@aol.com (Chilicatt) NNTP-Posting-Host: newsbf02.mail.aol.com I am looking for photos, artwork, etc. for a new diagnostic microbiology textbook (low royality or no royality). I appreciate any help you can offer and I will of course gratefully acknowledge anything that I am able to use. You can reach me at Chilicatt@aol.com Thanks! Chilicatt From owner-mycology@net.bio.net Mon Mar 13 22:00:00 1995 Path: biosci!adam.cc.sunysb.edu!news.nysernet.net!news.sprintlink.net!news.bluesky.net!solaris.cc.vt.edu!swiss.ans.net!prodigy.com!usenet From: VPGH51A@prodigy.com (Jim Berlstein) Newsgroups: bionet.mycology Subject: Re: Morels Date: 13 Mar 1995 23:58:38 GMT Organization: Prodigy Services Company 1-800-PRODIGY Lines: 18 Distribution: world Message-ID: <3k2m7e$132k@usenetw1.news.prodigy.com> NNTP-Posting-Host: inugap4.news.prodigy.com X-Newsreader: Version 1.2 In Regards to a morel found at UW- Are you sure it is a morchella genus morel and not a Verpa genus early morel? The Verpas are wrinkly, and the head is connected to the stem only at the top of the stem, and the stem is filled with a cottony material. The morchellas are PITTED, not wrinkled, and the head is joined to the stem at the base of the head, and the stem is hollow. Would you be willing to say where you found it? I was going down to the U on tuesday anyway, and I would like to look for some to photograph. I am planning my first trip to my favorite spot near portland next week, but I expect to find only verpas. Please pardon me if this information is an insult to your knowledge of morels. Happy hunting. - JIM BERLSTEIN VPGH51A@prodigy.com From owner-mycology@net.bio.net Mon Mar 13 22:00:00 1995 Path: biosci!news.cs.umb.edu!hsdndev!dartvax.dartmouth.edu!usenet From: Jennifer.Loros@dartmouth.edu (Jennifer Loros) Newsgroups: bionet.mycology Subject: Neurospora Methods Manual Date: 14 Mar 1995 20:54:37 GMT Organization: Dartmouth College, Hanover, NH Lines: 109 Message-ID: <3k4vqd$h4g@dartvax.dartmouth.edu> NNTP-Posting-Host: at-1-sn-178.dartmouth.edu X-Posted-From: InterNews 1.0.2@dartmouth.edu Neurospora Methods Manual An open letter of invitation to all Neurospora researchers, As we are all probably painfully aware, manuals of laboratory y protocols are usually written for and developed from a popular or widely used research system. Often, in today's research environment, that system is either mammalian cells in culture or yeast or maybe flies. While many of these methods can and have been successfully adapted in the laboratory for other model systems, like Neurospora, the time and effort involved in the adaptation process itself can be wearying if not prohibitive. In addition, there are many methods and techniques developed over the years that are specific to the genetics and physiology of this wonderful experimental system. Many of us working with Neurospora over the years have wished there was a collection of modern, easy to understand and replicate, laboratory methods specifically tailored to Neurospora. Preferably, only tried and truly useful methods would be included in a carefully screened and edited set of protocols that would systematically cover and address most approaches used to answer genetic, cell and molecular questions people are interested in asking today. Such an admirable collection of methods, providing a widely available and useful research tool world-wide for the practicing Neurosporologist, might be suitable for publication. The Neurospora Policy Committee was charged with this practical mission two years ago at the Fungal Genetics Meeting at Asilomar. We divided the responsibility among ourselves for collecting written protocols in a specified area of technique [very generally, DNA & RNA techniques, Libraries, Transformations including disruption and targeting, Cell fractionation , Culture in different contexts, Proteins & Microscopy]. We had a reasonably stringent set of rules for acceptance of a method including that each participating laboratory document the usefulness of each method by providing written evidence that said method was in successful use in at least one other laboratory. Our idea was to come up with something suitable for publication. Unfortunately, we have made slow progress in assembling a draft for a Neurospora laboratory methods book. Certainly, we will not have a draft ready for review by the community at the upcoming Asilomar workshop. While there is still an interest in working towards a publishable manual, the interests and needs of the Neurospora community may best be served on the short term by the immediate assembly of an informal collection of techniques in current use, hence this letter inviting all Neurospora laboratories to submit their favorite, tried and true, faithful recipes for laboratory success. At this time we plan no regular mailing so please pass this request on by phone or face-to-face communication with other labs. The needs of the community can be better served at this point by gathering a loose-leaf collection of commonly and successfully used protocols. If we start with an involved community and a loose-leaf collection of ever-expanding and up-dated protocols we might actually make the leap to a more formalized collection sometime in the future. In addition to "bench" benefits in the way of more successful experimentation, a further positive outcome might be that trading protocols will result in more informal communication and collaboration among Neurospora labs. We request: All labs with an interest in submitting a protocol (please don't be shy or think it sounds like too much work. The eventual success of this endeavor depends on an involved community) arrive at Asilomar at the Fungal Genetics Meeting with 50 Xeroxed copies of each submitted protocol. We intend to encourage participation in this way: at the meeting we will collect the protocols, and will trade one complete collection of each different protocol for your 50 copies of your submitted protocols-no protocol to submit, no collection to take home. We will distribute 1 set per lab which can be taken home and copied. Each method should preferably be 3 or less pages in length and type-written. Please punch them for a 3-ring binder. All labs with an interest in the collection arrive at Asilomar with an empty 3-ring binder. Protocols should be accompanied by appropriate references including; -Any literature references citing this protocol from the submitting lab or another lab. -Appropriate historical citation. (for example, many methods have been developed from or are adapted from either a published method or a method originating in some other lab). - Known use of protocols in other labs. -Names, including lab P.I., addresses, e-mail and snail-mail, and phone #'s. If you would like to participate but do not intend to attend the Asilomar meeting, please send me your name and address, along with your protocol(s). One last request. Eric Selker, Jay Dunlap and I all are enthusiastic about collecting a set of Neurospora experiments suitable for secondary and/or undergraduate biology teaching. As a teaching organism, Neurospora, with its lack of pathology, great genetics and highly visible development, both sexual and asexual, is excellent. If you have a lab already worked up, please bring a copy to Asilomar or send to me. If we get enough interested people we could put together a collection available to teachers. Sincerely, Jennifer Loros For the Neurospora Policy Committee Dept. of Biochemistry Dartmouth Medical School Hanover, NH 03755-3844 jennifer.loros@dartmouth.edu From owner-mycology@net.bio.net Mon Mar 13 22:00:00 1995 Path: biosci!rutgers!gatech!swrinde!cs.utexas.edu!uunet!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: MushroomPeople Apology Date: 14 Mar 1995 16:14:09 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 36 Sender: root@newsbf02.news.aol.com Message-ID: <3k50v1$564@newsbf02.news.aol.com> Reply-To: stamets1@aol.com (Stamets 1) NNTP-Posting-Host: newsbf02.mail.aol.com Folks- I am patiently awaiting an apology from MushroomPeople for the non-permissioned use of my Overview of Cultivation Techniques that they included in a mailing to their customer base advertising a March workshop by Bob Harris. They used this drawing without my permission, without acknowledgement or reference. As part of our legal settlement, they were to mail an apology to the same list which originally received the first mailer. Despite their assurances, I have not received the apology via the second mailing whereas I did receive the first one which illegally used my copyrighted material. Naturally, I wonder whether this second mailing was sent to the same customer base. I am simply asking: Has anyone received a mailing of the apology? I am asking members of Bionet.mycology for help in this matter. Thank you. Paul Stamets ps: On another matter, a group in New Zealand has copied portions of Growing Gourmet & Medicinal Mushrooms, including my photographs (and other contributors to this book) and are using them for advertising, instructional and other purposes. One correspondent said that bootleg copies of this book are also being distributed. Does anyone know anything about this ???? From owner-mycology@net.bio.net Mon Mar 13 22:00:00 1995 Path: biosci!sasa.gov.uk!burns From: burns@sasa.gov.uk (Robert Burns) Newsgroups: bionet.mycology Subject: antibodies from PAGE Date: 14 Mar 1995 09:06:39 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hi, has anybody out there used excised protein bands from PAGE gels to raise antibodies for use in fungal diagnostics? I was planning to try and find species-specific bands on gels and use the recovered peoteins as immunogens for Mab production. Any comments? Rob Robert Burns Monoclonal Antibody Unit Scottish Agricultural Science Agency East Craigs Edinburgh Scotland burns@sasa.gov.uk From owner-mycology@net.bio.net Mon Mar 13 22:00:00 1995 Path: biosci!rutgers!gatech!newsjunkie.ans.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: Re: Morels Date: 14 Mar 1995 10:33:37 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 4 Sender: root@newsbf02.news.aol.com Message-ID: <3k4d0h$1gs@newsbf02.news.aol.com> References: <3k2m7e$132k@usenetw1.news.prodigy.com> Reply-To: stamets1@aol.com (Stamets 1) NNTP-Posting-Host: newsbf02.mail.aol.com I am confident that if Tom O'Dell says he found a Morchella, he found a Morchella ! paul stamets From owner-mycology@net.bio.net Tue Mar 14 22:00:00 1995 Path: biosci!UMAIL.UMD.EDU!Jerome_J_MOTTA From: Jerome_J_MOTTA@UMAIL.UMD.EDU (jm102) Newsgroups: bionet.mycology Subject: Meeting Announcement Date: 15 Mar 1995 07:45:51 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 27 Sender: daemon@net.bio.net Distribution: world Message-ID: <9503151540.AA23890@umailsrv1.UMD.EDU> NNTP-Posting-Host: net.bio.net Announcement, Registration, and Call For Papers MID-ATLANTIC STATES MYCOLOGY CONFERENCE and FORAY May 6-7, 1995 The Pennsylvania State University University Park, PA FOR INFORMATION, REGISTRATION CONTACT: E.L Stewart Department of Plant Pathology The Pensylvania State Universtity 212-B Buckhout Laboratory University Park, PA 16802 Phone 814 865 7448 Fax 814 863 7271 e-mail els4@psu.ed or ebf@psuvm.psu.edu posted 3/15/95 J.Motta jm102@umail.umd.edu From owner-mycology@net.bio.net Tue Mar 14 22:00:00 1995 Path: biosci!VMS1.TAMU.EDU!dje0282 From: dje0282@VMS1.TAMU.EDU (Dan Ebbole) Newsgroups: bionet.mycology Subject: Neurospora Methods Manual Date: 15 Mar 1995 06:22:28 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Distribution: world Message-ID: <199503151422.GAA27346@net.bio.net> NNTP-Posting-Host: net.bio.net I believe there is a flaw in the plan suggested by Jennifer Loros for disseminating the protocol manual at Asilomar. Just because one does not have a rigorously tested protocol to share does not seem like a good justification for being excluded from receiving a copy of the protocols. I guess labs could bring something that sort of works sometimes so they could avoid being excluded, but that would be rather a waste of everyone's time. Certainly some folks will bring portable computers, so perhaps bringing 50 hard copies and a file on disk for mac or dos format (someone surely will should have a labtop that will read both formats to allow file copying at least). The collection of protocols should then be obtainable by request or by WWW from FGSC. ------------------------ Dr. Daniel Ebbole dje0282@summa.tamu.edu Dept. of Plant Pathology and Microbiology Texas A&M University College Station, TX 77843-2132 From owner-mycology@net.bio.net Tue Mar 14 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!swrinde!gatech!newsjunkie.ans.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: randbio@aol.com (RandBio) Newsgroups: bionet.mycology Subject: Re: MushroomPeople Apology Date: 15 Mar 1995 07:37:00 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 3 Sender: root@newsbf02.news.aol.com Message-ID: <3k6n1c$h79@newsbf02.news.aol.com> References: <3k50v1$564@newsbf02.news.aol.com> Reply-To: randbio@aol.com (RandBio) NNTP-Posting-Host: newsbf02.mail.aol.com I can offer no information regarding your apology, but I am interested in both publications mentioned in your posting. Please e-mail details on the title and availability. Thank you.....RR randbio@aol.com From owner-mycology@net.bio.net Tue Mar 14 22:00:00 1995 Path: biosci!rutgers!gatech!swrinde!cs.utexas.edu!news.sprintlink.net!oro.net!usenet From: brdotwn@oro.net (SlimeMold Technologies) Newsgroups: bionet.mycology Subject: Call for Flood Help Date: Tue, 14 Mar 95 23:54:27 GMT Organization: Darkside Dreamwalkers' Society Lines: 18 Message-ID: <3k5ihn$2u0@ag.oro.net> NNTP-Posting-Host: brdotwn.oro.net X-Newsreader: News Xpress Version 1.0 Beta #3 On the news tonight it was reported that more than a million almond trees have been blown over by the storms in California. I find this a hard number to believe. Still there are many-many trees down. These orchard owners will be very hard hit by this event. Of course being a mushroom enthusiast my thoughts turn to: "I wonder if there is a wonderful gourmet mushroom that can be grown on almond trees?" Okay mushroom folks. Is there a way that we can help these almond growers out and perhaps put mushroom culture more into the public eye? If they could be offered a low tech, easy to start mushroom culture that will use the millions of downed almond trees I think they would be very interested in listening. Claude brdotwn@oro.net From owner-mycology@net.bio.net Tue Mar 14 22:00:00 1995 Path: biosci!rutgers!gatech!swrinde!cs.utexas.edu!news.sprintlink.net!uunet!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: mycochef@aol.com (MYCOCHEF) Newsgroups: bionet.mycology Subject: Re: MOReLs Date: 14 Mar 1995 20:00:03 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 5 Sender: root@newsbf02.news.aol.com Message-ID: <3k5e6j$8lc@newsbf02.news.aol.com> References: <3jrstj$815@kelly.teleport.com> Reply-To: mycochef@aol.com (MYCOCHEF) NNTP-Posting-Host: newsbf02.mail.aol.com so far we have none to report, even up to the 3,500 foot elevation in the Sierras. Someone had reported finding a few dozen near Placerville, Ca. but this hunter was unable to locate any--and usually we do. . . .David A. did find some in downtown Monterey, Ca., two weeks ago but he is lucky that way. Weird almost. From owner-mycology@net.bio.net Tue Mar 14 22:00:00 1995 Path: biosci!bloom-beacon.mit.edu!grapevine.lcs.mit.edu!olivea!spool.mu.edu!howland.reston.ans.net!gatech!news-feed-1.peachnet.edu!insosf1.infonet.net!solaris.cc.vt.edu!news.duke.edu!eff!cs.umd.edu!news.umbc.edu!haven.umd.edu!hecate.umd.edu!usenet From: "J. Motta" Newsgroups: bionet.mycology Subject: MASMC CONFERENCE ANNOUNCEMENT - Jerome J. Motta Date: 15 Mar 1995 16:02:40 GMT Organization: University of Maryland, College Park Lines: 23 Message-ID: <3k7330$i0p@hecate.umd.edu> NNTP-Posting-Host: hjpatt-70.umd.edu Announcement and Call for Papers for THE MID-ATLANTIC STATES MYCOLOGY CONFERENCE AND FORAY MAY 6,7, 1995 The Pennsylvania State University University Park, PA For Information and Registration contact: Elwin L. Stewart Department of Plant Pathology The Pennsylvania State University 212-B Buckhout Laboratory University Par, PA 16802 Phone 814 865 7448 Fax 814 863 7217 e-mail els4@psu.edu or ebf@psuvm.psu.edu From owner-mycology@net.bio.net Tue Mar 14 22:00:00 1995 Newsgroups: bionet.mycology Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!ix.netcom.com!netcom.com!nnbt From: nnbt@netcom.com (Novo Nordisk Biotech) Subject: Hyphal tip isolations/strain purification Message-ID: Organization: NETCOM On-line Communication Services (408 261-4700 guest) X-Newsreader: TIN [version 1.2 PL1] Date: Wed, 15 Mar 1995 23:14:28 GMT Lines: 1 Sender: nnbt@netcom2.netcom.com From owner-mycology@net.bio.net Tue Mar 14 22:00:00 1995 Path: biosci!LABA.TDH.TEXAS.GOV!JHARRIS From: JHARRIS@LABA.TDH.TEXAS.GOV ("Jim Harris") Newsgroups: bionet.mycology Subject: Re: Workshops Date: 15 Mar 1995 14:27:53 -0800 Organization: Texas Department of Health LABs Lines: 35 Sender: daemon@net.bio.net Distribution: world Message-ID: <7541D1459@laba.tdh.texas.gov> NNTP-Posting-Host: net.bio.net Because there have been so many persons adding to the mycology group and because my e-mail address has changed this week, I am resubmitting the following workshop information for interested mycologists. If you have responded by e-mail prior to last week, I am confident that I received your inquiry and you should have received the printed information from me by now. Workshop #1 Fungi to Be Feared? Condo Contaminants and Toxic Toadstools Scheduled for May 18-19, 1995 in Washington, D.C. Presenters: L. Sigler, R. Summerbell, B. Jarvis, D. Miller, R. Gots, S. Epstein. Fee: Before April 15 is $200, after April 15, $250. Workshop #2 Identification of Significant Species of Aspergillus and Penicillium Scheduled for July 31-August 4, 1995 in San Antonio, TX Presenters: J. Pitt, M. Klich Fee: Before July 4 is $695, after July 4, $750 For more information on either of these programs, please send me your mailing address or a FAX number. (Or call me 512-458-7566) Jim Harris, Ph.D. Training Coordinator Bureau of Laboratories Texas Department of Health 1100 West 49th Street Austin, TX 78756 jharris@laba.tdh.state.tx.us From owner-mycology@net.bio.net Tue Mar 14 22:00:00 1995 Path: biosci!daresbury!not-for-mail From: "GORDON RUTTER" Newsgroups: bionet.mycology Subject: PhD Date: 15 Mar 1995 21:30:51 -0000 Organization: Royal Botanic Garden Edinburgh Lines: 8 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <3k7mab$8sv@mserv1.dl.ac.uk> X-mailer: WinPMail v1.0 (R2) Original-To: mycology@dl.ac.uk Dear mycologists, A quick question, Is there anyone out there who can offer me a PhD place, ideally on fungal taxonomy ideally basidiomycetes? If anyone has anything or any suggestions please email me direct and I can send my details to you. Thanks for your time Gordon Rutter From owner-mycology@net.bio.net Tue Mar 14 22:00:00 1995 Path: biosci!adam.cc.sunysb.edu!news.nysernet.net!news.sprintlink.net!howland.reston.ans.net!spool.mu.edu!eng.ufl.edu!usenet.eel.ufl.edu!newsmaster From: Steve Davies Newsgroups: bionet.mycology Subject: Kombucha or "Manchurian Mushroom" Date: 15 Mar 1995 20:57:32 GMT Lines: 3 Message-ID: <3k7kbs$2ie@huron.eel.ufl.edu> NNTP-Posting-Host: pc142.aec.env.gov.ab.ca I know this topic has been flogged to death lately but I recently received a request from an official in our Health Department (I am a research scientist working for the Government of Alberta in Western Canada) for information on Kombucha with regard to possible health concerns. I seem to recall postings of a couple of newspaper articles with reference to FDA concerns over use of this product; unfortunately I did not retain this information. I would appreciate any relevant info on the subject. Thank you. From owner-mycology@net.bio.net Tue Mar 14 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!news.sprintlink.net!uunet!in1.uu.net!dziuxsolim.rutgers.edu!er5.rutgers.edu!not-for-mail From: dubos@eden.rutgers.edu (Michelle Dubos) Newsgroups: bionet.mycology Subject: clubroot Date: 15 Mar 1995 15:22:17 -0500 Organization: Rutgers University Lines: 14 Distribution: world Message-ID: <3k7i9p$218@er5.rutgers.edu> NNTP-Posting-Host: er5.rutgers.edu Hello! Is anyone out there working with Plasmodiophora brassicae (or know of someone who is) ? I'm supposed to be working with it for my masters thesis and am running into a lot of problems. Many of them concern an inability to get infection in the greenhouse. I also would like to germinate the resting spores in the lab so I can work with the zoospores. ANY info concerning working with this organism would be GREATLY appreciated. Thanks. -Michelle dubos@eden.rutgers.edu From owner-mycology@net.bio.net Tue Mar 14 22:00:00 1995 Path: biosci!bloom-beacon.mit.edu!grapevine.lcs.mit.edu!olivea!spool.mu.edu!howland.reston.ans.net!news.cac.psu.edu!news.pop.psu.edu!hudson.lm.com!asia.lm.com!not-for-mail From: mspear@telerama.lm.com (mspear) Newsgroups: bionet.mycology Subject: Re: MushroomPeople Apology Date: 15 Mar 1995 11:56:44 -0500 Organization: Telerama Public Access Internet, Pittsburgh, PA Lines: 43 Message-ID: <3k768c$k64@asia.lm.com> References: <3k50v1$564@newsbf02.news.aol.com> NNTP-Posting-Host: asia.lm.com X-Newsreader: TIN [version 1.2 PL2] Stamets 1 (stamets1@aol.com) wrote: > Folks- > I am patiently awaiting an apology from MushroomPeople for the > non-permissioned use of my Overview of Cultivation Techniques that > they included in a mailing to their customer base advertising a March > workshop > by Bob Harris. They used this drawing without my permission, without > acknowledgement or reference. As part of our legal settlement, they were > to mail an apology to the same list which originally received the first > mailer. > Despite their assurances, I have not received the apology via the second > mailing whereas I did receive the first one which illegally used my > copyrighted > material. Naturally, I wonder whether this second mailing was sent to the > same > customer base. > I am simply asking: Has anyone received a mailing of the apology? > I am asking members of Bionet.mycology for help in this matter. > Thank you. > Paul Stamets > ps: On another matter, a group in New Zealand has copied portions of > Growing > Gourmet & Medicinal Mushrooms, including my photographs (and other > contributors to this book) and are using them for advertising, > instructional > and other purposes. One correspondent said that bootleg copies of this > book are also being distributed. Does anyone know anything about this ???? I saw one. ---- Mark mspear@sylvanres.com From owner-mycology@net.bio.net Tue Mar 14 22:00:00 1995 Path: biosci!bloom-beacon.mit.edu!grapevine.lcs.mit.edu!olivea!uunet!in1.uu.net!news.sprintlink.net!howland.reston.ans.net!usenet.ins.cwru.edu!cleveland.Freenet.Edu!fa609 From: fa609@cleveland.Freenet.Edu (Antonino G. Vetrano) Newsgroups: bionet.mycology Subject: Re: MushroomPeople Apology Date: 15 Mar 1995 15:37:11 GMT Organization: Case Western Reserve University, Cleveland, Ohio (USA) Lines: 18 Message-ID: <3k71j8$sep@usenet.INS.CWRU.Edu> NNTP-Posting-Host: kanga.ins.cwru.edu Dear Mr. Stamets: I am sure I saw an apology some weeks back but I am not sure if it was in FUNGUS Digest run by I believe R.Arnold at teleport.com. It was entitled "OOPS". I know I printed it out but I won't be able to search for it till Monday. If you know of Mr. Arnold and his FUNGUS DIGEST you might get faster results from him and his. When I find the copy I will send it by mail to you at FP. I also left a message with Mr. Arnold about this matter so he might be getting back to you. Just trying to help... T.Vetrano -- |-B^{> oB^{> dB^{> * -\B^{?> >B^{!> P^{> {B^{> ;^{> | A. G. Vetrano, fa609@cleveland.freenet.edu | Specialization | | is for Insects |<}c8 <}^| <}^O <}^: NNTP-Posting-Host: net.bio.net By popular request, I am posting a registration form and tentative schedule for the May 6,7 MASMC meeting and foray. Information on accomodations is available by request. We appreciate all of the interest! Mycology IS alive and well. Jerry Motta ......................................................................... REGISTRATION AND CALL FOR PAPERS MID-ATLANTIC STATES MYCOLOGY CONFERENCE MAY 6-7, 1995 Registration Deadline: April 10, 1995 Name and Mailing Address ________________________________________________ FAX: __________________ ________________________________________________ PHONE: __________________ ________________________________________________ E-MAIL: __________________ ________________________________________________ Registration Fees: Regular Registration (Includes Barbeque) $40.00 _____ Student Registration (Includes Barbeque) 30.00 _____ Barbeque only 20.00 _____ _____ I plan to attend the field trip on May 7, 1995 +------------------------------------------------------------------------+ | I plan present a paper (15 minutes) | | | | Title of Paper: ___________________________________________________| | ___________________________________________________________________| | | | Do you need a slide projector? ______ Overhead projector? _______ | | | +------------------------------------------------------------------------+ Send to: Elwin I. Stewart Department of Plant Pathology The Pennsylvania State Unvieristy 212-B Buckhout Laboratory University Park, PA 16802 814 865 7448 FAX 814 863 7217 e-mail - Elwin Steward: els4@psu.edu Barbara Christ: ebf@psuvm.psu.edu Make Checks Payable to: MASMC (Mid-Atlantic States Mycology Conference) ........................................................................ snip snip snip snip snip snip snip MID - ATLANTIC STATES MYCOLOGY CONFERENCE Date: May 6-7, 1995 Location The Pennsylvania State University 101 Animal Science & Industry (ASI) Building University Park, Pennsylvania Tentative Schedule: May 6, 1995 (Saturday) 8:00 a.m. - 9:00 a.m. Registration 8:30 a.m. - 12:00 noon Papers 12:00 noon - 1:pm p.m. Lunch 1:00 p.m. - 4:30 p.m, Papers 4:30 p.m. - 5:00 p.m. Business meeting 5:30 p.m. - Outdoor Barbeque May 7, 1995 (Sunday) 9:00 a.m. - 12:00 Field Trip  From owner-mycology@net.bio.net Wed Mar 15 22:00:00 1995 Path: biosci!ORCHID.UCSC.EDU!bowman From: bowman@ORCHID.UCSC.EDU Newsgroups: bionet.mycology Subject: Fungal Genetics meeting Date: 16 Mar 1995 10:36:31 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: <9503161835.AA09509@biology.UCSC.EDU> TO: those planning to come to the meeting at Asilomar CA, 21-26 March. FROM B. Bowman, UC Santa Cruz. Several people have asked about road and weather conditions. Because of the recent storms, several roads into the Monterey Peninsula are closed. However, usable detours are available in most cases. The most serious problem is that the bridge is gone over the Carmel river on the coast highway (Rt 1). Those coming from the south should not use RT 1. It may also be difficult to get to Point Lobos. If you come down from San Francisco, Rt 1 is also closed at the area known as Devil's Slide (well named). You can take Rt 1 if you take 92 to Half Moon Bay and then proceed south. Between Santa Cruz and Asilomar, Rt 1 is closed near Watsonville, but there is a detour around this problem. As of today (11PM Wed 16 March) the sun is shining and the weather is beautiful. Good weather is predicted through Monday. Some of the road problems should be fixed by the start of the meeting. From owner-mycology@net.bio.net Wed Mar 15 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!news.sprintlink.net!pipex!sunsite.doc.ic.ac.uk!dundee.ac.uk!mban560.scri.sari.ac.uk!Mail From: Mail@scri.sari.ac.uk Newsgroups: bionet.mycology Subject: New BSPP web server (British Society for Plant Pathology Date: Thu, 16 Mar 1995 16:55:40 Organization: Scottish Crop Research Institute Lines: 10 Message-ID: NNTP-Posting-Host: 134.36.144.229 Keywords: BSPP Plant pathology www X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] A new web server for the British Society for Plant Pathology (BSPP) has been established at the Scottish Crop Research Institute. The URL is http://www.scri.sari.ac.uk/bspp Helpful comments to a.newton@scri.sari.ac.uk Many documents have yet to be written. Please be patient! Adrian Newton From owner-mycology@net.bio.net Wed Mar 15 22:00:00 1995 Path: biosci!rutgers!gatech!swrinde!cs.utexas.edu!news.tamu.edu!NewsWatcher!user From: aat3230@zeus.tamu.edu (Gus Trautweiler) Newsgroups: bionet.mycology,bionet.molbio.methds-reagnts Subject: Advice on mitochondria isolation from fungi (i.e. percoll gradient). Date: Thu, 16 Mar 1995 17:26:04 +0800 Organization: Texas A&M University Lines: 13 Message-ID: NNTP-Posting-Host: 128.194.248.26 Xref: biosci bionet.mycology:1747 bionet.molbio.methds-reagnts:26039 We are about to isolate mitochondria using both a sucrose gradiant and percoll gradient from two fungi, Mycosphaerella graminicola and Stagonospora nodorum. Our protocol tells us to suspend the crude mito-pellet in a 0.8M sucrose solution prior to centrifugation. Is it any different with the percoll gradient? Any other advice is welcome including preparing/grinding of cells. Please respond by email....THANK YOU! Gus Trautweiler ###QRNQURNQ##*. .*#*. .*#*. .*#*. Plant Pathology & Microbiology * | | | * * | | | * | | | * Texas A&M University * * | | | * * | | | * * | | | * College Station, TX 77843-2132 * | | | * | | | * * | | | * AAT3230@ZEUS.TAMU.EDU ###*' `*#*' `*#*' `*#*' From owner-mycology@net.bio.net Thu Mar 16 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!msunews!harbinger.cc.monash.edu.au!news.cs.su.oz.au!metro!news.une.edu.au!usenet From: Mathew Campbell Newsgroups: bionet.mycology Subject: Suicide Fungi Date: 17 Mar 1995 08:12:03 GMT Organization: University of New England, NSW, Australia Lines: 8 Message-ID: <3kbg8j$7cl@grivel.une.edu.au> NNTP-Posting-Host: kenny.une.edu.au Has anyone read any recent references on the use of suicide fungi in biocontrol, i.e. the use of fungi which are applied with a nutrient adjuvant as bioherbicides. If so could you PLEASE let me know the relevant references. Thanks in Advance, Mathew Campbell Department of Botany University of New England Armidale, NSW, Australia 2351. From owner-mycology@net.bio.net Thu Mar 16 22:00:00 1995 Newsgroups: bionet.mycology Path: biosci!rutgers!gatech!swrinde!howland.reston.ans.net!ix.netcom.com!netcom.com!nnbt From: nnbt@netcom.com (Novo Nordisk Biotech) Subject: hyphal tips Message-ID: Organization: NETCOM On-line Communication Services (408 261-4700 guest) X-Newsreader: TIN [version 1.2 PL1] Date: Fri, 17 Mar 1995 01:18:29 GMT Header: hyphal tips Lines: 17 Sender: nnbt@netcom12.netcom.com Are there any experts out there with (successful) experience in hyphal tip isolations? I would be interested to hear from anyone who has used the technique for obtaining "purified" strains/transformants. Perhaps you know of alternatives for obtaining colonies derived from propagules/hyphal fragments containing single nuclei in species with multinucleate spores? If so, and you would like to share the information, please mail me at the address below. Thanks! Wendy T. Yoder E-mail: windy@nnbt.com Novo Nordisk Biotech Inc, 1445 Drew Avenue Davis CA 95616 USA From owner-mycology@net.bio.net Thu Mar 16 22:00:00 1995 Path: biosci!rutgers!gatech!news-feed-1.peachnet.edu!news.duke.edu!solaris.cc.vt.edu!swiss.ans.net!prodigy.com!usenet From: VPGH51A@prodigy.com (Jim Berlstein) Newsgroups: bionet.mycology Subject: Re: The Cyberspace Homeless Date: 17 Mar 1995 19:28:41 GMT Organization: Prodigy Services Company 1-800-PRODIGY Lines: 17 Distribution: world Message-ID: <3kcnt9$88m@usenetw1.news.prodigy.com> NNTP-Posting-Host: inugap4.news.prodigy.com X-Newsreader: Version 1.2 In regards to Theo's pitiful plea for purchasing power, I have come up with my own more mycologically relevant rant regarding rubles- Dear theo and others, I want to spend all my time roaming the woods picking mushrooms. You can send me money so I can do this, or you can turn the page. Boo-hoo Nobody loves me c',mon mister, spare some change, huh? Will hunt mushrooms for food. - JIM BERLSTEIN VPGH51A@prodigy.com From owner-mycology@net.bio.net Thu Mar 16 22:00:00 1995 Path: biosci!cabi.org!J.PRYSE From: J.PRYSE@cabi.org ("Janet Pryse ", Training Officer) Newsgroups: bionet.mycology Subject: Courses in mycology Date: 17 Mar 1995 08:30:05 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 138 Sender: daemon@net.bio.net Distribution: world Message-ID: <2F69B88F@msm.cgnet.com> NNTP-Posting-Host: net.bio.net INTERNATIONAL MYCOLOGICAL INSTITUTE The International Mycological Institute (IMI) is the world's largest centre for systematic, applied and environmental mycology. Operating from its new purpose-built campus at Egham on the south-west edge of London, IMI organises a wide range of courses in agricultural, industrial, environmental and systematic mycology and bacteriology covering identification, characterization, isolation and preservation techniques and methods. Brief details of courses available in 1995 are given below. For further details on any of these, please indicate the course(s) of interest and send your postal address to: Miss Janet Pryse (Training Officer) International Mycological Institute Bakeham Lane Egham, Surrey TW20 9TY UK Tel +44 (0) 1784 470111 Fax +44 (0) 1784 470909 Email j.pryse@cabi.org Diagnostic Techniques in Plant Pathology 12 - 23 June 1995 An intensive course with a high practical content, providing a full introduction to the theory and practice of the broad range of techniques used in investigative plant pathology. The course is intended for practising field plant pathologists, quarantine officers, plant clinic officers etc., who wish to widen and enhance their skills in the diagnosis of diseases caused by plant pathogenic fungi, bacteria, viruses and nematodes. The course aims to provide a thorough coverage of the elements of the diagnostic process, including symptom recognition, examination of diseased material, isolation, culture and preliminary identification of possible causal agents and confirmation of their disease-causing ability. Course Fee ?950 Modern Techniques in the Identification of Bacteria and Filamentous Fungi 26 June - 7 July 1995 This two week course is designed to give microbiologists/plant pathologists training in modern techniques currently of use in microbial taxonomy. The course will include lectures and practical work, with emphasis on gaining "hands on" experience of the techniques themselves. Both molecular biological and chemotaxonomic procedures will be used to study filamentous fungi and bacteria of agricultural significance. Topics to be covered include: - RFLP analyses - PCR detection and characterisation methods (including RAPD's) - Detection and identification techniques using serological methods - Characterisation of microorganisms by polyacrylamide gel electrophoresis - Analysis of whole-cell proteins - Analysis of extra- and intracellular isozyme patterns for identification - Identification of bacteria by quantitative fatty acid analysis - Analysis of isoprenoid quinones - Diagnostic uses of secondary metabolites (eg phytotoxins, mycotoxins) by TLC, HPLC Course Fee ?1000 International Course on the Identification of Fungi of Agricultural Importance 7 August - 15 September 1995 A long established six week course giving plant pathologists training in the classification and identification of agriculturally important groups of microfungi. The course will be of particular relevance to those working in the tropics. The training includes lectures and informal discussions, but most of the time will be spent in practical work. A number of 'option' days throughout the course enable participants to receive individual training and to specialize in certain aspects of the Institute's work. Course Fee ?2600 to include self catering accommodation. This course is held each year. Basic Mycological Techniques 23 & 24 October 1995 A short introductory course aimed at microbiology technicians or others needing help with traditional mycological techniques. It will be suitable for those working in plant pathology, industry, food or environmental mycology. It will be particularly useful for new technical staff or those unused to working with fungi. Isolation from plant material, soil and manufactured goods will be covered. Topics will include the preparation of a variety of material for microscopic examination, culturing and aseptic techniques, methods of inducing sporulation and the use and preparation of standard and specialised media. The course will consist of lectures and demonstrations but most time will be spent in experience building practical sessions. There will be ample opportunity for discussion of any particular mycological problems which participants may have encountered. Course fee ?275 - For participants also registered for our Culture Preservation Techniques course, 25-27 October, a combined discounted fee o