From owner-mycology@net.bio.net Sat Apr 01 23:00:00 1995
Path: biosci!bcm!cs.utexas.edu!news.sprintlink.net!news.bluesky.net!solaris.cc.vt.edu!swiss.ans.net!prodigy.com!usenet
From: VPGH51A@prodigy.com (Jim Berlstein)
Newsgroups: bionet.mycology
Subject: Re: **FAST CASH**
Date: 2 Apr 1995 00:36:35 GMT
Organization: Prodigy Services Company  1-800-PRODIGY
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I no speak so good,
but I send 1 dried morel out to 5 people and I get back
50,000 pounds of dried morels!
I want share good fortune,
this is for reel,
and monkeys are flying out of my butt,
no, really, they are,
why would I lie?


-
  JIM BERLSTEIN  VPGH51A@prodigy.com




From owner-mycology@net.bio.net Sat Apr 01 23:00:00 1995
Path: biosci!RUMAC.UPR.CLU.EDU!C_BETANCOURT
From: C_BETANCOURT@RUMAC.UPR.CLU.EDU
Newsgroups: bionet.mycology
Subject: CMI
Date: 2 Apr 1995 06:10:02 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
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I would like to know to acces the Commonwealth Mycological Institute
databases.Please send the information to my E_mail

From owner-mycology@net.bio.net Sat Apr 01 23:00:00 1995
Path: biosci!agate!howland.reston.ans.net!spool.mu.edu!torn!news.unb.ca!peinet.pe.ca!peinet.pe.ca!not-for-mail
From: stewart@bud.peinet.pe.ca (Paul Stewart)
Newsgroups: bionet.mycology
Subject: straw culture
Date: 2 Apr 1995 15:44:32 -0400
Organization: PEINet, Charlottetown, Prince Edward Island, Canada
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	I am currently growing a few dozen bottles of grain spawn of 
oyster mushrooms, and plan to inoculate pasteurized straw. As this is my 
first attempt, I would like to float a couple of questions to the group.
I could not find detailed answers to these questions in Stamet's latest book.

	1.  How does one cut the straw into the desired lengths without 
expensive farm machinery?  I have access to a chipper which a friend 
uses to chop apples prior to cider pressing, but he says when he tried 
straw in it, the straw wasn't so much cut as "puffed up". 

	2.  I plan to dump the pasteurized straw on bleach-cleaned 
plastic sheeting, inoculate with grain spawn, roll the sheet into a tube, 
tie the ends with wire, and hang the tube in a plastic-partitioned grow 
room in the loft of my garage/barn. Will this work? I just don't have the 
cash for "space bags" right now. How cold can such a mixture be and still 
grow?

	3.  In the interest of obtaining slants or spores from others 
within Canada or USA, what are the regulations governing transport or 
mailing of spawn/spores?

	4.  All I can find on PEI is barley/oats straw.  Not much wheat 
grown here, and the straw is ploughed back in.  Stamets favors wheat 
straw. Is there anything I can supplement the barley/oat straw with to 
bring it up to scratch?

	5.  The grain I am using is also barley/oats.  Not much rye grown 
here and what I've found is quite expensive.  I know about 50% moisture 
is good for grain spawn, but I steeped/boiled the damn stuff for two 
hours and couldn't get the weight of 100 mL of the stuff to double. Best 
I could do was 35%, which seems awfully low. Anyone else use this grain?
Also, when cooled the next day, thousands of tiny white dots was seen on 
the grain surface. Is this some form of contamination or a normal 
phenomenon of boiled grain?  It appeared on the inoculated and unopened 
bottles both. 

	6.  I drilled holes in the mason cover, and put a piece of filter 
paper (Whatman #42 Ashless) over it before sterilizing. I sterilize at 15 
lbs for 1.5 hours, and autoclave tape placed in the center of the grain 
jar turned black. Will this filter paper be sufficient to keep te interior 
sterile?

	I will be most grateful for any replies...

				Paul from the Great Thawing Northeast

From owner-mycology@net.bio.net Sun Apr 02 23:00:00 1995
Newsgroups: bionet.mycology
Path: biosci!agate!library.ucla.edu!csulb.edu!csus.edu!netcom.com!yarrum
From: yarrum@netcom.com (murcohen)
Subject: Kambucha???
Message-ID: <yarrumD6G4L3.7o7@netcom.com>
Organization: NETCOM On-line Communication Services (408 261-4700 guest)
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Date: Mon, 3 Apr 1995 06:27:03 GMT
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Can someone refer me to an article on this fungus and tea and the health 
benefits, if any, derived from it?  Please Email to:
						yarrum@netcom.com
					thanks....
-- 
                                             yarrum@netcom.com

From owner-mycology@net.bio.net Sun Apr 02 23:00:00 1995
Path: biosci!daresbury!not-for-mail
From: Alfiya Fattakhova <fata@charlie.ksu.ras.ru>
Newsgroups: bionet.mycology
Subject: e-mail Phytopathology
Date: 3 Apr 1995 15:43:38 +0100
Lines: 2
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Message-ID: <3lp1iq$jh2@mserv1.dl.ac.uk>
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Original-To: mycology@dl.ac.uk

Dear netters, I am seeking e-mail or mail adress of Phytopathology journal,I need the Order for authers.
Please if somebody know, help me. My Internet: fata@charlie.ksu.ras.ru

From owner-mycology@net.bio.net Sun Apr 02 23:00:00 1995
Path: biosci!cabi.org!D.BRAYFORD
From: D.BRAYFORD@cabi.org ("David Brayford ", IMI)
Newsgroups: bionet.mycology
Subject: RE: CMI
Date: 3 Apr 1995 00:48:59 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
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Hi
We are the International Mycological Institute nowadays. (This is because 
our parent company, CAB International, is a treaty level International 
organisation with an intergovernmental governing board, not restricted to 
members of the British Commonwealth).

At present most of our databases are not available for dial-in access, with 
the exception of our Culture Collection catalogue via the Microbial Strain 
Data Network (ask MSDN@PHX.CAM.AC.UK).  However, CAB International is 
planning a WWW page in the near future and IMI will be part of that. 
 Meanwhile, what information are you wanting to access?  Maybe we can help 
out.

For info, please note that we have a new address:
International Mycological Institute
Bakeham Lane
Egham, Surrey
TW20 9TY
UK
Phone: (01784) 470111
FAX: (01784) 470909
Email:  IMI@CABI.ORG

You can reach particular IMI mycologists here via email by taking their 1st 
initial, a dot, their surname, followed by @CABI.ORG.  The same goes for our 
plant pathology, industrial, biochemical, bacteriological and food mycology 
staff.  If you want information on CABI databases, such as CAB Abstracts, 
ask CABI@CABI.ORG.

Let me know what you are looking for and I'll try to help out.  Please 
include your email address as part of the text of your message, otherwise it 
just comes through as "BIOSCI-REQUEST".

Dave Brayford, IMI
d.brayford@cabi.org

 ----------
From: BIOSCI-REQUEST
To: mycology
Subject: CMI
Date: 02 April 1995 6:10

I would like to know to acces the Commonwealth Mycological Institute
databases.Please send the information to my E_mail


From owner-mycology@net.bio.net Sun Apr 02 23:00:00 1995
Path: biosci!KOKO.CSUSTAN.EDU!jshuman
From: jshuman@KOKO.CSUSTAN.EDU (Jeremy Shuman)
Newsgroups: bionet.mycology
Subject: (none)
Date: 3 Apr 1995 09:10:05 -0700
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From owner-mycology@net.bio.net Sun Apr 02 23:00:00 1995
Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!spool.mu.edu!torn!news.bc.net!rover.ucs.ualberta.ca!finzi.ccinet.ab.ca!news@ccinet.ab.ca
From: ksanders@ccinet.ab.ca (Ken Sanderson)
Newsgroups: bionet.mycology
Subject: Re: black morel and ...
Date: 3 Apr 1995 22:59:10 GMT
Organization: CCI Networks, a division of Corporate Computers Inc.
Lines: 20
Message-ID: <3lpuju$1s4@finzi.ccinet.ab.ca>
References: <9503301956.AA12716@sunblock.wec.com>
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X-Newsreader: WinVN 0.90.3

In article <9503301956.AA12716@sunblock.wec.com>, mh@SUNBLOCK.PGH.WEC.COM (Mike Hoffelder) says:
>
>Ken Sanderson <ksanders@ccinet.ab.ca> wrote:
>
>>   ..... What is the best way to get rid of the worms
>>   I find in even the youngest morel?
>
>What kind of worms do you find in morels?!?!
>I have been gathering morels for 25 years,
>and have found very few pests inhabiting morels.
>I have found only a couple of slugs and a few 
>wood lice in my whole 25 year morel experience.
>

The worms I find are small white ones approx  5-10 mm long (1/4 inch).  
I have found them in abundance in almost all morels I find here (northern
Alberta, Canada) even very small ones.  It has somewhat ruined the idea of
eating them.

Ken Sanderson

From owner-mycology@net.bio.net Sun Apr 02 23:00:00 1995
Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!spool.mu.edu!olivea!decwrl!nntp.crl.com!crl5.crl.com!not-for-mail
From: mitymik@crl.com (Mick Ishmael)
Newsgroups: bionet.mycology
Subject: Hi...I'm a clueless newbie!
Date: 3 Apr 1995 09:53:15 -0700
Organization: CRL Dialup Internet Access	(415) 705-6060  [Login: guest]
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X-Newsreader: TIN [version 1.2 PL2]

at least when it comes to mushrooms.Where can i find the FAQ...and can 
someone tell me about them things i see at the grocery (common commercial 
mushrooms)?

I remember a TV program a while bact that said you could grow a certain 
variety of mushroom on (hickory charcoal?)...anybody know what i'm 
refering to?

thanx.

From owner-mycology@net.bio.net Sun Apr 02 23:00:00 1995
Path: biosci!KOKO.CSUSTAN.EDU!jshuman
From: jshuman@KOKO.CSUSTAN.EDU (Jeremy Shuman)
Newsgroups: bionet.mycology
Subject: Subscribe
Date: 3 Apr 1995 09:07:02 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 3
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Distribution: world
Message-ID: <9504031607.AA23199@koko.csustan.edu>
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I would like to subscribe to the mycology newsgroup by mail.

thanks.

From owner-mycology@net.bio.net Mon Apr 03 23:00:00 1995
Path: biosci!UNIX1.SNCC.LSU.EDU!esiegel
From: esiegel@UNIX1.SNCC.LSU.EDU (Eric Robb Siegel)
Newsgroups: bionet.mycology
Subject: Re:  HELP: Fungal nuclei isolation
Date: 3 Apr 1995 20:49:23 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
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you could make fungal protoplasts, and then try using on them some of the techniques used for getting subcellular fractionation of plant material.  I do not have any of the latter at my fingertips to cite, but not only do they isolate
chloroplasts all the time, they also do "nuclear runoff" experiments with 
isolated plant nuclei.

From owner-mycology@net.bio.net Mon Apr 03 23:00:00 1995
Path: biosci!daresbury!trane.uninett.no!Norway.EU.net!EU.net!howland.reston.ans.net!cs.utexas.edu!news.tamu.edu!128.194.103.15!leland
From: leland@straylight.tamu.edu (Leland Ellis)
Newsgroups: bionet.mycology
Subject: Microbial Strain Data Network (MSDN)
Followup-To: bionet.mycology
Date: 04 Apr 1995 16:13:24 GMT
Organization: W.M. Keck Center for Genome Informatics, Institute of Biosciences
	and Technology, Texas A&M University, Houston
Lines: 84
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Message-ID: <LELAND.95Apr4111324@straylight.tamu.edu>
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Hello, the following announcement for a WWW page for the Microbial
Strain Data Network (MSDN) appeared on the biodiv-l Email ListServer.
The MSDN, which resides at the Base de Dados Tropical in Campinas (Sao
Paulo, Brasil), contains quite a few pointers to data sources,
incl. several for fungi.

Cheers,

Leland

P.S.:

From biodiv-l@ftpt.br Tue Apr  4 04:38 CDT 1995
Return-Path: <biodiv-l@ftpt.br>
Errors-To: dora@ftpt.br
Date: Mon, 3 Apr 95 13:36:23 EST
Errors-To: dora@ftpt.br
Reply-To: biodiv-l@ftpt.br
Originator: biodiv-l
Sender: biodiv-l@ftpt.br
Precedence: bulk
From: dora@ftpt.br (Dora Ann Lange Canhos)
To: Multiple recipients of list <biodiv-l@ftpt.br>
Subject: MSDN web page
X-Listserver-Version: 6.0 -- UNIX ListServer by Anastasios Kotsikonas
X-Comment: Biodiversity Information Network List
Content-Type: text
Content-Length: 1666

The Microbial Strain Data Network (MSDN) has launched its web
server at BDT. The location is http://www.bdt.org.br/msdn/msdn.html

This server includes the following databases:

   Bacterial Nomenclature (DSM's compilation of IJSB)
   Fungi from Brazil Recorded by Batista and Co-Workers
   American Type Culture Collection (link to ATCC web server)
   AVIS Animal Virus Information System
   Brazilian Catalogues
   CCALA Czech Algae and Cyanobacteria
   CCM Czech Bacteria
   CCF Czech Filamentous Fungi
   DSM Deutsche Sammlung von Mikroorganismen 
   IBSO Siberian Luminous Bacteria 
   KMMGU Russia Bacteria 
   IMI International Mycological Institute 
   IPPAS Russia Algae 
   MSU Russia Yeasts 
   NCYC UK National Collection Yeasts 
   PETERHOF Genetic Collection S.cerevisiae Yeasts 
   LEBIN Russia Basidiomycetes 
   RIAM Russia Fungi 
   Slovenia Filamentous Fungi
   VKM Russia  of Bacteria, Fungi and Yeasts
   VKM Russia Genetically Modified Organisms
   VKPM Russia  of Industrial Organisms (Bacteria, Fungi, Yeasts, Actinomycetes)
   Bacterial Identification Matrices
   EBIS European Community Biotechnology Information Service
   Microbial Strain Data Network International Directory

This server also has links to:

   WFCC World Data Centre on Microorganisms (WDCM)
   Bioline Publications
   IRRO Databases on Environmental Releases


-- 
-----------------------------------------------------------
Dora Ann Lange Canhos         E-mail: dora@bdt.org.br
Base de Dados Tropical        Tel:    +55 192 427022
Fundacao "Andre' Tosello"     Fax:    +55 192 427827
(http://www.bdt.org.br)
-----------------------------------------------------------


--

**************************************************************************
 Leland Ellis, Ph.D. -- Director, W.M. Keck Center for Genome Informatics
 Institute of Biosciences and Technology, Texas A&M University, Houston
 Email:	leland@straylight.tamu.edu
 WWW:	http://straylight.tamu.edu/straylight.html
**************************************************************************

From owner-mycology@net.bio.net Mon Apr 03 23:00:00 1995
Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!pipex!peernews.demon.co.uk!nova.novanet.org!harringtonj-smtc
Newsgroups: bionet.mycology
From: harringtonj-smtc@nova.novanet.org (harringtonj-smtc)
Subject: Re: straw growing
X-Posting-Host: novacon.cerl.uiuc.edu
Date: Tue, 4 Apr 1995 06:16:00 +0000
Message-ID: <199504040618.AA20725@novacon.cerl.uiuc.edu>
Sender: usenet@demon.co.uk
Lines: 50

Paul,
 
Regarding growing out the Oyster mushrooms, IMHO,
 
1. Chopping the straw: A chipper won't do it. Chippers are
for wood. You need a shredder, or chipper/shredder. If none
available, spread your straw out on a flat soil surface
and work it over with a lawn mower.
 
2. Rolling you own plastic tubing is risky. You need
a TIGHT, COMPACT medium for the mycelium to colonize. You
will probably end up with some air gaps or pockets that
will contaminate. For your first go at it, you might
consider some sturdy 4 mil plastic bags, about a cubic
foot in size. Much easier to handle. Your rolled plastic
tube might rip apart when you hang it up, especially after
you puncture it for the fruit to bloom through?
 
3. Temps for spawn run etc:  You need to follow the
parameters for the temp/humidity fairly closely. If its too
low, you risk contamination from competitors. Stamets give
the parameters for Ostreatus P. in his book. Copy success.
Maximize your growing area. If you have a space heater
(electric) available, put a line voltage thermostat in-line
on the extension cord. That way you can maintain the
required temps for the different stages.
 
4. Straws:  OAT straw should be fine. Wheat is preferred
because of its structure, but OAT is ok. Dr. Ralph
Kurtzman, USDA plant pathologist, was one of the pioneers
with Oyster cultivation, in the 1970's.  He wrote that he
had poor results with barley. Stamets said barley was
acceptable. Both agreed OAT is fine.
 
5. SPAWN GRAIN:  Again, OAT grain should be fine. Its not
clear how you are sterilizing it? It MUST be done in a
pressure cooker, at the specified times/pressures. Boiling
in a open atomosphere won't make it. Soak it overnight
before you autoclave it.
 
6. Inoculation rates: inoculate pasterized straw at minimum
of 5% or dry weight of straw, i.e., dry straw + 100 lbs,
add 5 # of grain spawn, ideally 10 # (10 %). Higher
inoculation rate will result in faster colonization.
 
Hope this helps!
 
Joe
 
Portland, Maine

From owner-mycology@net.bio.net Mon Apr 03 23:00:00 1995
Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!ix.netcom.com!netnews
From: DITTO70@ix.netcom.com (MARK PLOTKIN)
Newsgroups: bionet.mycology
Subject: Cordyceps
Date: 4 Apr 1995 04:20:22 GMT
Organization: Netcom
Lines: 7
Distribution: world
Message-ID: <3lqhe6$4le@ixnews4.ix.netcom.com>
NNTP-Posting-Host: ix-dc6-02.ix.netcom.com

All,
I'm a newcomer to this newsgroup looking for popular articles on life 
history and medicinal applications of Cordyceps suitable for high school 
and college level audiences. Any suggestionswould be most appreciated.

F. Bonifay


From owner-mycology@net.bio.net Mon Apr 03 23:00:00 1995
Path: biosci!IDPTV.IDBSU.EDU!pub907
From: pub907@IDPTV.IDBSU.EDU (Warren Rekow)
Newsgroups: bionet.mycology
Subject: Re: straw culture (Paul Stewart)
Date: 4 Apr 1995 09:51:33 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 47
Sender: daemon@net.bio.net
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Message-ID: <m0rwBoC-0000T6C@idptv.idbsu.edu>
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>         1.  How does one cut the straw into the desired lengths without
> expensive farm machinery?  ....

One can get good growth of Oysters on straw without cutting or shredding.
The innoculated straw should be tightly packed to increase the density.

>         2.  I plan to dump the pasteurized straw on bleach-cleaned
> plastic sheeting, inoculate with grain spawn, roll the sheet into a tube,
> tie the ends with wire, and hang the tube in a plastic-partitioned grow
> room in the loft of my garage/barn. Will this work? I just don't have the
> cash for "space bags" right now. How cold can such a mixture be and still 
> grow?

That would probably work fine, though just packing the straw into new
unused garbage bags of whatever size might be an easier approach. In either
case be sure to poke many holes into the container soon after filling.
Temperature requirements vary with the strain or species, but the high 60s
to low 70s would probably be good.  See Stamets' books.

> 4... Is there anything I can supplement the barley/oat straw with to 
> bring it up to scratch?

You should be able to get fruiting without supplementing the straw.
Oysters can grow on many cellulosic substrates.

> 5.  The grain I am using is also barley/oats. ....

If the grain is real clean with a low spore count as evidenced by few or
no contaminated jars, and if the grain kernels do not get gummed together
after autoclaving, then it should work.  Some people use wheat or millet.
If the white spots are contamination you will probably soon know by
observing non-white growths, places devoid of mycelia, or an unpleasant
odor. A good procedure is to soak the grain overnight prior to autoclaving.
This both hydrates the grain, and gives contaminant spores a chance to
germinate so that they will be more susceptible to kill by autoclaving.

> 6.  I drilled holes in the mason cover, and put a piece of filter 
> paper (Whatman #42 Ashless) over it ....
> Will this filter paper be sufficient to keep te interior sterile?

Per above, you will soon know.  It seems like Oyster or other mycelia
would be able to degrade the paper.  Fungi Perfecti sells filter disks
and plastic jar lids which work well and endure a long time.
-- 
Warren Rekow (pub907@idptv.idbsu.edu)
HC 79, Box 104, Melba  ID  83641
Phone or fax: 208-896-4908

From owner-mycology@net.bio.net Tue Apr 04 23:00:00 1995
Path: biosci!adam.cc.sunysb.edu!news.sprintlink.net!howland.reston.ans.net!news.cac.psu.edu!psuvm!hgw1
Organization: Penn State University
Date: Wed, 5 Apr 1995 10:20:47 EDT
From: Heather Treaster <HGW1@psuvm.psu.edu>
Message-ID: <95095.102047HGW1@psuvm.psu.edu>
Newsgroups: bionet.mycology
Subject: Grad. Assistantships Available
Lines: 14

Graduate Assistantships Available.  The Penn State Plant Pathology
Department has several assistantships available for M.S.  or Ph.D.
students.  Faculty programs include a diverse range of research in basic
and applied plant pathology, including: field and greenhouse crop
pathology, forest pathology, sustainable agriculture, molecular fungal
taxonomy, mushroom culture and pathology, fungal genetics, epidemiology,
virology, plant responses to environmental stress, air pollution effects
to forest trees and ecosystems, root-specific metabolism, root-pathogen
interactions, host-parasite interactions.  Excellent facilities
available for field work and biochemical/molecular biology research.
Contact Dr. J.E.  Ayers, Graduate Admissions Committee, Plant Pathology
Department, 211 Buckhout Laboratory, University Park, PA 16802 (Phone:
814-865-7069; Fax: 814-863-7217; E-mail: htreaste@psupen.psu.edu).
Affirmative Action/Equal Opportunity Employer; women and minorities
encouraged to apply.

From owner-mycology@net.bio.net Wed Apr 05 23:00:00 1995
Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.cac.psu.edu!news.tc.cornell.edu!travelers.mail.cornell.edu!newstand.syr.edu!rodan.syr.edu!jworrall
From: jworrall@rodan.syr.edu (James J. Worrall)
Newsgroups: bionet.mycology
Subject: Referring to members of higher taxa
Date: 6 Apr 1995 14:59:09 GMT
Organization: Syracuse University, Syracuse NY
Lines: 22
Message-ID: <3m0vjt$hus@newstand.syr.edu>
NNTP-Posting-Host: rodan.syr.edu

I would be grateful for opinions from mycologists, especially
systematists, on acceptable ways to refer to members of
certain higher taxa.  For instance, if we believe in a class
Ascomycetes, most of us get in the habit of using sentences
like: "Ascomycetes produce asci," as if the class were a plural
reference to all members, rather than saying,  "Members of the
Ascomycetes produce asci."

If we believe instead that the group should be recognized at the
division level, we have a choice of:
"Ascomycota produce asci," which sounds bad;
"Members of the Ascomycota produce asci,"
"Ascomycetes produce asci," using a lower-case informal term; or
"Ascomycotans produce asci," as Kendrick did in his book.

None of these seem completely satisfactory.  Using the term
"ascomycetes" to refer to members of the division Ascomycota
may be a bad habit and is certainly confusing to students.

Any opinions out there?  Thanks.

Jim Worrall

From owner-mycology@net.bio.net Wed Apr 05 23:00:00 1995
Path: biosci!adam.cc.sunysb.edu!news.sprintlink.net!howland.reston.ans.net!news.moneng.mei.com!uwm.edu!newsspool.doit.wisc.edu!news.doit.wisc.edu!F180-124.net.wisc.edu!jmicales
From: jmicales@facstaff.wisc.edu (Jessie Micales)
Newsgroups: bionet.mycology
Subject: Re: Referring to members of higher taxa
Date: Thu, 6 Apr 1995 17:56:15 GMT
Organization: U.S. Forest Service
Lines: 32
Message-ID: <jmicales.191.2F842B3E@facstaff.wisc.edu>
References: <3m0vjt$hus@newstand.syr.edu>
NNTP-Posting-Host: f180-124.net.wisc.edu

>I would be grateful for opinions from mycologists, especially
>systematists, on acceptable ways to refer to members of
>certain higher taxa.  For instance, if we believe in a class
>Ascomycetes, most of us get in the habit of using sentences
>like: "Ascomycetes produce asci," as if the class were a plural
>reference to all members, rather than saying,  "Members of the
>Ascomycetes produce asci."

>If we believe instead that the group should be recognized at the
>division level, we have a choice of:
>"Ascomycota produce asci," which sounds bad;
>"Members of the Ascomycota produce asci,"
>"Ascomycetes produce asci," using a lower-case informal term; or
>"Ascomycotans produce asci," as Kendrick did in his book.

>None of these seem completely satisfactory.  Using the term
>"ascomycetes" to refer to members of the division Ascomycota
>may be a bad habit and is certainly confusing to students.

>Any opinions out there?  Thanks.

>Jim Worrall

Although I'm not a mycologist in the pure, classical sense, it has always 
been my belief that your original statement is proper (ascomycetes produce 
asci), except that you would use lower case "a" for ascomycetes. I recently 
had a manuscript returned from a reviewer who changed "Basidiomycete" to 
"basidiomycete" in the same type of situation.  The singular form would 
also be correct (e.g. Cryphonectria is an ascomycete).

I will be interested in seeing other people's responses.


From owner-mycology@net.bio.net Wed Apr 05 23:00:00 1995
Path: biosci!bcm!cs.utexas.edu!news.swt.edu!pirates.cs.swt.edu!swrinde!emory!usenet
From: djdlab@bimcore.emory.edu (Dean Danner Lab)
Newsgroups: bionet.mycology
Subject: UCSF fungi?
Date: 6 Apr 1995 22:04:22 GMT
Organization: Biomolecular Computing Resource, Emory University
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Reply-To: mlanterm@gmm.gen.emory.edu
NNTP-Posting-Host: bimcore.emory.edu

Is there anyone at UCSF doing some fun things with fungi?  Need a post-doc?  Please write me if you know anything/one:
mlanterm@gmm.gen.emory.edu
Margaret Lanterman




From owner-mycology@net.bio.net Wed Apr 05 23:00:00 1995
Path: biosci!bloom-beacon.mit.edu!grapevine.lcs.mit.edu!chaos.dac.neu.edu!news3.near.net!bigboote.WPI.EDU!wpi.WPI.EDU!crusberg
From: crusberg@wpi.edu (Theodore C. Crusberg)
Newsgroups: bionet.mycology
Subject: Cell Wall Breakage
Date: 6 Apr 1995 19:49:37 GMT
Organization: Worcester Polytechnic Institute
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I would like to break through the cell walls of a Penicillium sp. to
rescue a periplasmic enzyme.  I have used a number of enzymes to
digest the cell wall.  Recently we read about a method in which the
mycelia is lyophilized and then treated with diluted ammonium
hydroxide.  Has anyone tried this?  Are there any better techniques?
Thanks,
Ted Crusberg
Biology & Biotechnology
Worcester Polytechnic Institute


From owner-mycology@net.bio.net Wed Apr 05 23:00:00 1995
Path: biosci!BSCR.UGA.EDU!arnold
From: arnold@BSCR.UGA.EDU
Newsgroups: bionet.mycology
Subject: (none)
Date: 6 Apr 1995 13:00:54 -0700
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NNTP-Posting-Host: net.bio.net

Physical maps of the Aspergillus nidulans
genome are available by anonymous ftp from

fungus.genetics.uga.edu


  . - - - - - - - - - - - Jonathan Arnold - - - - - - - - - - - - - - - .
  |                       Dept. of Genetics,                            |
  |                       University of Georgia                         |
  |                       Athens, Georgia 30602                         |
  | Phone:       (706) 542-1449                                         |
  | messages:    (706) 542-9359                                         |
  | FAX:         (706) 542-3910                                         |     
  | Internet:    ARNOLD@BSCR.UGA.EDU                                    |
  . - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - .


From owner-mycology@net.bio.net Thu Apr 06 23:00:00 1995
Newsgroups: bionet.mycology
Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.moneng.mei.com!uwm.edu!reuter.cse.ogi.edu!netnews.nwnet.net!ns1.nodak.edu!badlands!jweiland
From: jweiland@badlands.NoDak.edu (John J Weiland)
Subject: Pyrenophora mating--observations.
Sender: usenet@ns1.nodak.edu (Usenet login)
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Date: Fri, 7 Apr 1995 13:31:34 GMT
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First of all, thanks to all who responded to queries about mating of 
Pyrenophora in culture.  I wish to relay some interesting results so 
far.  The most common protocol relayed to me for mating of P.teres was to 
get mycelia of opposite mating types growing on Sach's agar with bits of 
sterile barley straw as perithecia substrate.  Then place in dark at 
10-15C for several months, and chack for maturation of asci along the way.

Here's the interesting part---after 3 months (it is now the first week in 
April) only one of the 25 plates that were seeded for each cross is 
maturing now, and it is REALLY cranking out the ascospores.  This plate 
was treated differently from the others, however.  It was brought home 
one week after initial seeding (mycelia had already invaded the straw and 
protoperithecia were evident) and placed in a west-facing bedroom that 
was un-heated (this is northern Minnesota, USA).  The plates were not 
exposed to direct sunlight, but were near a closed window.  Temperatures 
in this room probably ranged from 5C to 15C and the daylength was 
naturally increasing with the coming of spring.

So, maybe those in the southern hemisphere may wish to try this---the 
experiment could be to plate some matings now since the days are growing 
shorter, and compare the results to platings after June 21.  Please 
post/Email comments if you have any questions.

John Weiland
Department of Plant Pathology
NDSU
Fargo,   North Dakota   USA

From owner-mycology@net.bio.net Thu Apr 06 23:00:00 1995
Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!pipex!uknet!daresbury!not-for-mail
From: Alfiya Fattakhova <fata@charlie.ksu.ras.ru>
Newsgroups: bionet.mycology
Subject: conferences?
Date: 7 Apr 1995 14:17:40 +0100
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Dear netters ! Das anybody know what confernces will be in the future in mycology and phytopathology ?
please, e-mail: fata@charlie.ksu.ras.ru

From owner-mycology@net.bio.net Thu Apr 06 23:00:00 1995
Path: biosci!bcm!cs.utexas.edu!news.sprintlink.net!pipex!oleane!jussieu.fr!fdn.fr!uunet!newsfeed.ACO.net!fstgds15.tu-graz.ac.at!usenet
From: Michael Murkovic <f548murk@mbox.tu-graz.ac.at>
Newsgroups: bionet.mycology
Subject: solid state fermentation
Date: 7 Apr 1995 09:02:10 GMT
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I've a problem in determination of fungal biomass in 
solid state fermentation. Can anyone give me a hint?


thanks
mike

From owner-mycology@net.bio.net Thu Apr 06 23:00:00 1995
Path: biosci!newshost.lanl.gov!news.ttu.edu!aurora.LaTech.edu!darwin.sura.net!news.mci.net!news.mid.net!news.ksu.ksu.edu!vixen.cso.uiuc.edu!uwm.edu!newsspool.doit.wisc.edu!news.doit.wisc.edu!F180-200.net.wisc.edu!jmicales
From: jmicales@facstaff.wisc.edu (Jessie Micales)
Newsgroups: bionet.mycology
Subject: Re: Cell Wall Breakage
Date: Fri, 7 Apr 1995 13:36:53 GMT
Organization: U.S. Forest Service
Lines: 19
Message-ID: <jmicales.192.2F853FF5@facstaff.wisc.edu>
References: <3m1gkh$i79@bigboote.WPI.EDU>
NNTP-Posting-Host: f180-200.net.wisc.edu

>I would like to break through the cell walls of a Penicillium sp. to
>rescue a periplasmic enzyme.  I have used a number of enzymes to
>digest the cell wall.  Recently we read about a method in which the
>mycelia is lyophilized and then treated with diluted ammonium
>hydroxide.  Has anyone tried this?  Are there any better techniques?
>Thanks,
>Ted Crusberg
>Biology & Biotechnology
>Worcester Polytechnic Institute

Lyophilization can denature some enzymes.  I've never had much luck with it 
myself.  I usually use a mortar and pestle to crush hyphae in liquid 
nitrogen, perhaps with some glass beads (although they usually aren't 
necessary).  This seems to work sufficiently for what I do (enzyme 
extractions from basidiomycetes).

Jessie Micales
Forest Products Laboratory
Madison, WI

From owner-mycology@net.bio.net Thu Apr 06 23:00:00 1995
Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.sprintlink.net!matlock.mindspring.com!jemini.mindspring.com!jeb
From: jeb@mindspring.com (Jeb Howell)
Newsgroups: bionet.mycology
Subject: Mycology Definitive bible or Key
Date: Thu, 6 Apr 1995 21:12:08 -0400
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I am not a mushroom person, but my brother has a keen interest in the subject, 
and would like me to inquire of the group if there is a single best reference 
book about the culture of and identification of North American mushrooms. Any 
input would be appreciated. My email address is jeb@mindspring.com.

THANKS MUCHLY!!!

From owner-mycology@net.bio.net Fri Apr 07 23:00:00 1995
Path: biosci!adam.cc.sunysb.edu!news.sprintlink.net!howland.reston.ans.net!news.cac.psu.edu!news.pop.psu.edu!hudson.lm.com!india.lm.com!not-for-mail
From: mspear@telerama.lm.com (mspear)
Newsgroups: bionet.mycology
Subject: Re: solid state fermentation
Date: 7 Apr 1995 21:48:16 -0500
Organization: Telerama Public Access Internet, Pittsburgh, PA
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Michael Murkovic (f548murk@mbox.tu-graz.ac.at) wrote:
> I've a problem in determination of fungal biomass in 
> solid state fermentation. Can anyone give me a hint?


> thanks
> mike

Classic problem faced by many.  I know only of rough solutions.  A
couple of years ago Romaine at PSU developed an ELISA (I think) that
he could use to quantify cells.  I have seen claims for an ATP assay. 
Of course these are only very indirect measures.

I tend to appoach it more from a "How many cells?" point of view than
a "What mass?" point of view.  This can be assayed by serial dilution
plating or inoculation effeciency tests.  

How about a MPN assay?

    ---- Mark

From owner-mycology@net.bio.net Fri Apr 07 23:00:00 1995
Path: biosci!daresbury!trane.uninett.no!Norway.EU.net!EU.net!uunet!hearst.acc.Virginia.EDU!portal.gmu.edu!osf1.gmu.edu!hkilpatr
From: hkilpatr@osf1.gmu.edu (HENRY E. KILPATRICK JR.)
Newsgroups: bionet.mycology
Subject: Morels in the Mid-Atlantic
Date: 7 Apr 1995 14:00:22 GMT
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A few morels were found in the Washington, DC area on March 26.
It's been cold and dry lately, so I doubt there have been many more 
fruitings.  

--
Buddy Kilpatrick

From owner-mycology@net.bio.net Fri Apr 07 23:00:00 1995
Newsgroups: bionet.mycology
Path: biosci!adam.cc.sunysb.edu!news.sprintlink.net!howland.reston.ans.net!torn!news.bc.net!vanbc.wimsey.com!unixg.ubc.ca!nntp.cs.ubc.ca!news.UVic.CA!news.pfc.forestry.ca!PFC.Forestry.CA!RWINDER
From: rwinder@PFC.Forestry.CA (Richard Winder)
Subject: Re: e-mail Phytopathology
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References: <3lp1iq$jh2@mserv1.dl.ac.uk>
Distribution: bionet
Date: Fri, 7 Apr 1995 16:15:13 GMT
Lines: 12

In article <3lp1iq$jh2@mserv1.dl.ac.uk>, Alfiya Fattakhova 
<fata@charlie.ksu.ras.ru> writes:
>Dear netters, I am seeking e-mail or mail adress of Phytopathology journal,
>I need the Order for authers.
>Please if somebody know, help me. My Internet: fata@charlie.ksu.ras.ru

E-mail apsnet@scisoc.org


  RICHARD WINDER                    Title: Research Scientist
  Canadian Forest Service           Phone: (604) 363-0773
  Victoria, B.C.                    Internet: RWINDER@A1.PFC.Forestry.CA

From owner-mycology@net.bio.net Fri Apr 07 23:00:00 1995
Path: biosci!daresbury!trane.uninett.no!Norway.EU.net!EU.net!howland.reston.ans.net!news.sprintlink.net!uunet!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: cavetocave@aol.com (CAVETOCAVE)
Newsgroups: bionet.mycology
Subject: Re: Hi...I'm a clueless newbie!
Date: 6 Apr 1995 03:23:32 -0400
Organization: America Online, Inc. (1-800-827-6364)
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Don't eat the common commercial one if you are trying to avoid commercial
toxins.
Because of the problems of growing out of compost, fungicides (for the
lower fungus) and pesticides )for the flying inscects eggs/larva are used.
Eat shiitake' or tree oyster or wild from the woods types and you will not
have that problem.
Also if taste is a priority, same same.

From owner-mycology@net.bio.net Fri Apr 07 23:00:00 1995
Path: biosci!daresbury!trane.uninett.no!nac.no!Norway.EU.net!EU.net!news.sprintlink.net!uunet!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: cavetocave@aol.com (CAVETOCAVE)
Newsgroups: bionet.mycology
Subject: Re: Kambucha???
Date: 6 Apr 1995 03:22:50 -0400
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NNTP-Posting-Host: newsbf02.mail.aol.com

Mushroom The Journal ran a very good article by Paul Stamets who regularly
posts on this board.

From owner-mycology@net.bio.net Fri Apr 07 23:00:00 1995
Newsgroups: bionet.mycology
Path: biosci!adam.cc.sunysb.edu!news.sprintlink.net!howland.reston.ans.net!torn!news.bc.net!vanbc.wimsey.com!unixg.ubc.ca!nntp.cs.ubc.ca!news.UVic.CA!news.pfc.forestry.ca!PFC.Forestry.CA!RWINDER
From: rwinder@PFC.Forestry.CA (Richard Winder)
Subject: Re: straw culture
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References: <3lmrag$le0@bud.peinet.pe.ca>
Date: Fri, 7 Apr 1995 16:08:45 GMT
Lines: 21

In article <3lmrag$le0@bud.peinet.pe.ca>, stewart@bud.peinet.pe.ca 
(Paul Stewart) writes:
>
>	3.  In the interest of obtaining slants or spores from others 
>within Canada or USA, what are the regulations governing transport or 
>mailing of spawn/spores?
>
>

As far as I know, you only have a problem if the fungus you are
importing is considered pathogenic- a glance in the ATCC catalogue will
give you that information.  For example, A. bisporus would be fine, but
Armillaria (root rot) would require an import permit from Ag. Canada.  
If you're considering getting a lot of cultures from the U.S., it might be good
to touch base with Ag. Canada anyway.  Other than that concern, if there is a 
commercial value to the culture, you may have to pay duty.  -RSW


  RICHARD WINDER                    Title: Research Scientist
  Canadian Forest Service           Phone: (604) 363-0773
  Victoria, B.C.                    Internet: RWINDER@A1.PFC.Forestry.CA

From owner-mycology@net.bio.net Sat Apr 08 23:00:00 1995
Path: biosci!newshost.lanl.gov!news.ttu.edu!aurora.LaTech.edu!darwin.sura.net!spool.mu.edu!bloom-beacon.mit.edu!grapevine.lcs.mit.edu!uhog.mit.edu!rutgers!uwm.edu!reuter.cse.ogi.edu!netnews.nwnet.net!news.u.washington.edu!homer04.u.washington.edu!todell
From: Thomas O'Dell <todell@u.washington.edu>
Newsgroups: bionet.mycology
Subject: Re: solid state fermentation
Date: Sat, 8 Apr 1995 10:42:13 -0700
Organization: University of Washington
Lines: 7
Message-ID: <Pine.A32.3.91c.950408103818.120024B-100000@homer04.u.washington.edu>
References: <3m2v2i$9e8@fstgds15.tu-graz.ac.at> <3m4thg$ne5@india.lm.com>
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Ergosterol can be assayed and used to estimate of fungal biomass.
See the chapter by J-E Nylund in Methods in Microbiology 1992 edited by
Read, D.; Norris and Varma. There were two vols. with those editors that 
were dedicated to mycorrhizal techniques...
Email me directly for the complete citation (its at the office).
todell@u.washington.edu


From owner-mycology@net.bio.net Sat Apr 08 23:00:00 1995
Newsgroups: bionet.mycology
Path: biosci!news.cs.umb.edu!oitnews.harvard.edu!news.dfci.harvard.edu!camelot.ccs.neu.edu!chaos.dac.neu.edu!usenet.eel.ufl.edu!spool.mu.edu!howland.reston.ans.net!agate!dog.ee.lbl.gov!news.cs.utah.edu!emba-news.uvm.edu!lemming.uvm.edu!resnik
From: Robert Resnik <resnik@lemming.uvm.edu>
Subject: Re: Kambucha??? 
In-Reply-To: <yarrumD6G4L3.7o7@netcom.com> 
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On Mon, 3 Apr 1995, murcohen wrote:

> Can someone refer me to an article on this fungus and tea and the health 
> benefits, if any, derived from it?  Please Email to:
> 						yarrum@netcom.com
> 					thanks....
> -- 
>                                              yarrum@netcom.com

There was an article about kambucha and its reputed benefits in a 
magazine called Advocate about 2 issues ago (it was probably the February 
issue).

   **************************************************
   |  Robert J. Resnik      resnik@lemming.uvm.edu  |
   |  Outreach & Reference Librarian                |
   |  Fletcher Free Library                         |
   |  235 College Street     Burlington, VT 05401   |
   |  Phone (802) 865-7222   FAX   (802) 865-7227   |
   **************************************************


From owner-mycology@net.bio.net Sat Apr 08 23:00:00 1995
Path: biosci!daresbury!bioftp.unibas.ch!citi2.fr!jussieu.fr!oleane!pipex!howland.reston.ans.net!news2.near.net!news.delphi.com!loyl
From: loyl@delphi.com (Loy Lawhon)
Newsgroups: bionet.mycology
Subject: Bread mold
Date: 8 Apr 1995 11:52:41 GMT
Organization: Delphi Internet Services Corporation
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NNTP-Posting-Host: bos1e.delphi.com
X-To: Loy Lawhon <loyl@delphi.com>

 
    Hello,

    I have a question. It may seem dumb, but I am not a biologist.
Why does white bread mold more quickly than whole-wheat or rye bread?
Thanks in advance for your help.

    Loy : loyl@delphi.com

 

From owner-mycology@net.bio.net Sat Apr 08 23:00:00 1995
Path: biosci!UNIX1.SNCC.LSU.EDU!esiegel
From: esiegel@UNIX1.SNCC.LSU.EDU (Eric Robb Siegel)
Newsgroups: bionet.mycology
Subject: Fluorescence microscopy on Neurospora
Date: 9 Apr 1995 14:30:02 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
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Sender: daemon@net.bio.net
Distribution: world
Message-ID: <9504092129.AA124351@unix1.sncc.lsu.edu>
NNTP-Posting-Host: net.bio.net


I am trying to find out if anyone has used any of the Molecular Probes reagents 
to look at, for example, Vegetative Incompatibility in Neurospora.  I recently 
ran "fluoresc* and neurospora" as search words through the Science Citation 
Index databases back through 1989 (the earliest that we have, other than print),
and I came up with surprisingly little.  I do not know if this is an accurate 
reflection of what is in the literature, or if it merely means I was not imagin-
ative enough in my use of search terms.  No, I haven't thought to call Molecular
Probes, to ask them directly, until just now while composing this netmessage.
Still, I would be interested to hear from you what you know.

Eric Siegel

From owner-mycology@net.bio.net Sat Apr 08 23:00:00 1995
Path: biosci!SSD.INTEL.COM!branan
From: branan@SSD.INTEL.COM (Nancy Branan)
Newsgroups: bionet.mycology
Subject: mushrooms buyers
Date: 9 Apr 1995 16:49:49 -0700
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Message-ID: <9504092349.AA01862@SSD.intel.com>
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We are searching for names and contacts of (large) buyers of wild mushrooms
in the U.S.A., Europe and Japan.

Please contact Bruce Branan,
tel/fax (503) 614-0590
branan@ssd.intel.com

____________________________________________________________________

Nancy Branan                                    branan@ssd.intel.com
Customer Support                                tel: 503-629-7737
Intel Supercomputers                            fax: 503-629-6324
____________________________________________________________________


From owner-mycology@net.bio.net Sat Apr 08 23:00:00 1995
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From: mspear@telerama.lm.com (mspear)
Newsgroups: bionet.mycology
Subject: Re: Hi...I'm a clueless newbie!
Date: 9 Apr 1995 19:28:45 -0400
Organization: Telerama Public Access Internet, Pittsburgh, PA
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CAVETOCAVE (cavetocave@aol.com) wrote:
> Don't eat the common commercial one if you are trying to avoid commercial
> toxins.
> Because of the problems of growing out of compost, fungicides (for the
> lower fungus) and pesticides )for the flying inscects eggs/larva are used.
> Eat shiitake' or tree oyster or wild from the woods types and you will not
> have that problem.
> Also if taste is a priority, same same.

I disagree.  Commercial Agaricus farmers are just like any other
farmers.  There are some who use a lot of pesticides.  However the
successful ones got that way by keeping costs down and quality and
productivity up.  As in most crops this means minimal pesticides,
Integrated Pest Management, sanitary practices instead of poisons.

The average Agaricus operation is around 4 million pounds per year. 
It's got lot's of employees and has the usual constraints of FIFRA
regulations, insurance inspections, FDA rules and the like.

The average shiitake or oyster grower is much smaller, not nearly as
professional, has much less time in the business, and can "fly under
the radar".  Pest and disease control without poisons are not as well
worked out for the exotics.  In short these guys tend to use whatever
they need to and can get away with it.

The blanket assertion that Agaricus is pesticide laden and shiitake is
not probably should be discarded.

    ---- Mark

From owner-mycology@net.bio.net Sat Apr 08 23:00:00 1995
Path: biosci!ns1.faseb.org!darwin.sura.net!mother.usf.edu!madonna!schatzow
From: schatzow@madonna.ec.usf.edu. (Robert Schatzow (LFD))
Newsgroups: bionet.mycology
Subject: Thanks
Date: 9 Apr 1995 18:12:20 GMT
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About 1 or 2 months ago I asked for some help in finding a rare fungus 
that a professor here had contracted.  I received numerious responses.

Thanks for all of your help, the professor is doing fine now.  The 
information was very valuable.

Bob Schatzow

--
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^  
Robert S. Schatzow                                 FIRN:schatzr
Student Computer Lab Supervisor          Internet:schatzr@mail.firn.edu
Hillsborough Community College          

From owner-mycology@net.bio.net Sun Apr 09 23:00:00 1995
Path: biosci!agate!howland.reston.ans.net!pipex!oleane!jussieu.fr!fdn.fr!uunet!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: fitzdc@aol.com (FitzDC)
Newsgroups: bionet.mycology
Subject: want to grow 'em
Date: 9 Apr 1995 23:19:02 -0400
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I live in the southeast and have property with a spring and a creek.
Hence, high humidity and lots of perpetual ground moisture in parts. There
is always alot of native fungi around, but I'd like to know if it's
possible to "seed"(or should I say "spore") for particular types of
edibles. Also, where would I purchase the stuff to do so?

From owner-mycology@net.bio.net Sun Apr 09 23:00:00 1995
Path: biosci!rutgers!gatech!howland.reston.ans.net!news.cac.psu.edu!news.tc.cornell.edu!travelers.mail.cornell.edu!newstand.syr.edu!kong.syr.edu!griffin
From: griffin@kong.syr.edu (David H. Griffin)
Newsgroups: bionet.mycology
Subject: Re: Referring to members of higher taxa
Date: 10 Apr 1995 19:07:47 GMT
Organization: SUNY-ESF, Syracuse, NY
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The root -mycetes comes from the Greek word mykes (or myces), depending 
on how you want to transliterate the third letter of the alphabet, kappa. 
"Myces" is the singular nominative form and "mycetes" is the plural 
nominative form. Therefore making a pseudosingular form by dropping the 
final s is clearly an anglicisation of the word, and incorrect by the 
rules of grammar for the formal taxonomic name. When referring to one 
fungus as a member of a group, it would be better to say, for example, 
"Neurospora is a member of the Ascomycetes." The statement, "Neurospora 
is an Ascomycetes," has wrong number relationships. To drop the s, as in 
ascomycete, makes this Greek word incorrect as such, and should only be 
used informally, not as the name of a taxon. Thus, "Neurospora is an 
ascomycete." But then your reader cannot be sure if you mean to accept 
Ascomycetes as a class, or that you are just making a descriptive statement.

From owner-mycology@net.bio.net Sun Apr 09 23:00:00 1995
Path: biosci!EMAIL.PSU.EDU!djr4
From: djr4@EMAIL.PSU.EDU ("Dan Royse")
Newsgroups: bionet.mycology
Subject: Re: Workshop
Date: 10 Apr 1995 10:09:58 -0700
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Announcement

The 6th Annual Specialty Mushroom Workshop, June 13-15, 1995 will be held on 
Penn State's University Park (State College, PA) campus.  The Workshop is 
designed to promote the exchange of ideas for mushroom production, merchandising
and research.

Highlights

This year we will host the popular finger food mixer with the Agaricus Mushroom 
Group on Tuesday evening, June 13.  The informal atmosphere provides an 
excellent opportunity to renew old friendships and discuss the latest happenings
as well as make some new friends.  This year will also feature a field trip on 
Wednesday, June 14, with participants in the Mushroom Short Course to visit a 
mushroom packaging company and a composting company located near Kennett Square,
Pennsylvania.

The June 15th Program Includes:

* Marketing of Specialty Mushrooms and Production of Enokitake in the United 
States; Rod Sorenson
* Promotional Activities with Mushrooms in North America; Robyn Wilk
* Marketing and Other Challenges Facing the Specialty Mushroom Industry in the 
United States and Abroad; Robert Johns
* Trends in the Development of Production Technology for Specialty Mushrooms; 
Daniel J. Royse
* Toward Consistent Production of Shiitake and Pleurotus in Pennyslvania; Tim 
Hihn
* Status of Insects and Insect Management for Specialty Mushrooms; Shelby 
Fleischer
* Maitake and Shiitake Production in the Northeastern United States; Eric Lee
* Medicinal Aspects of Ganoderma and Shiitake; S. C. Jong
* Group Discussion and Tour of Mushroom Research Center

Registration

The registration fee for the Workshop is $165 ($185 after May 15) and includes 
tuition, tour, beverage breaks, and mixer.  Registration will be accepted by 
mail or fax through May 30, 1995.  Walk-in registrants will be accepted only as 
space allows.  Meals, tours and special events must be preregistered.  Refunds 
will be made in full if cancellation is received by June 1, 1995.  For 
cancellations after this date, a fee of $50 will be deducted before refunding.

For further information
About course content:
Daniel J. Royse, Chair
Specialty Mushroom Workshop
The Pennsylvania State University
316 Buckhout Laboratory
University Park, PA 16802-4507
Fax: 814/863-7217

About course registration:
Specialty Mushroom Workshop
The Pennsylvania State University
306 Ag. Adminsistration Building
University Park, PA 16802-2601
814/865-8301; Fax 814/865-7050; TDD 814/865-1204



From owner-mycology@net.bio.net Sun Apr 09 23:00:00 1995
Path: biosci!qmrelay.mail.cornell.edu!wolfram_koeller
From: wolfram_koeller@qmrelay.mail.cornell.edu ("Wolfram Koeller")
Newsgroups: bionet.mycology
Subject: Position Announcement
Date: 10 Apr 1995 07:45:56 -0700
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                       Subject:                               Time:9:34 AM
  OFFICE MEMO          Position Announcement                  Date:4/10/95

POSITION ANNOUNCEMENT

Postdoctoral position.  The New York State Agricultural Experiment Station in
Geneva as part of Cornell University is seeking a Postdoctoral Associate to
work on the characterization, cloning and mutation of a mitochondrial gene of
fungi as the target for a new class of antifungals.  A PhD degree, competence
in fungal molecular genetics and familiarity with cloning techniques are
required.  The position is funded for two years and is available immediately. 
Apply to:  Dr. Wolfram Koeller, Department of Plant Pathology, New York State
Agricultural Experiment Station, Geneva, NY 14456.  Phone;  315/787-2375. 
Fax:  315/787-2389.  Cornell University is an equal opportunity/affirmative
action employer.



From owner-mycology@net.bio.net Sun Apr 09 23:00:00 1995
Newsgroups: bionet.mycology
Path: biosci!agate!howland.reston.ans.net!pipex!uknet!comlab.ox.ac.uk!ivpc011.nerc-oxford.ac.uk!rel
From: rel@mail.nerc-oxford.ac.uk
Subject: Pythium infection process
Message-ID: <rel.1.000A7A6D@mail.nerc-oxford.ac.uk>
Lines: 16
Organization: Natural Environment Reseach Council
X-Newsreader: Trumpet for Windows [Version 1.0 Rev A]
Date: Mon, 10 Apr 1995 10:28:37

Can anyone help me?  I've recently started a studentship on the biocontrol of 
Pythium damping off on peas.  I'm having trouble finding out the true mode of 
infection of this pathogen.  It appears to me that mycologists spend a great 
deal of time studying zoospore infection processes whilst plant pathologists 
don't appear to consider this and instead concetrate their efforts on the 
hyphal mode of attack.  Can anyone provide anything that might help me resolve 
this problem?

Secondly, I'd like to mark the Pythium strain I have to study its in vivo 
distibution.  Can anyone advise me on how to transform this organism?

Thanks in advance

Richard Ellis

 

From owner-mycology@net.bio.net Sun Apr 09 23:00:00 1995
Path: biosci!VMS2.TAMU.EDU!dje0282
From: dje0282@VMS2.TAMU.EDU (Dan Ebbole)
Newsgroups: bionet.mycology
Subject: gene designations in Neurospora-changes?
Date: 10 Apr 1995 13:03:31 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
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I am inclined to suggest to the Neurospora policy committee that we
consider changing gene designations to match current usage for S.
cerevisiae.  There are many reasons for this, most of them are obvious. 
Currently a gene such as cpc-1 is written (in italics) cpc-1, the mutant is
(in italics) cpc-1-, the protein is CPC1.  In S. cerevisiae usage this
would be the gene (in italics) CPC1, a mutant, (in italics) cpc1 and the
protein Cpc1p.  

Comments?
------------------------
Dr. Daniel Ebbole
dje0282@summa.tamu.edu
Dept. of Plant Pathology and Microbiology
Texas A&M University
College Station, TX 77843-2132


From owner-mycology@net.bio.net Sun Apr 09 23:00:00 1995
Newsgroups: bionet.mycology
Path: biosci!rutgers!gatech!howland.reston.ans.net!spool.mu.edu!torn!nott!emr1!news
From: Kharrison (Ken Harrison)
Subject: Re: Help with Boletus retipes? ornatipes?
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In article <44496.rarnold@teleport.com>, rarnold@teleport.com says...
>
>I received this posting to FUNGUS.  Can you help? 
>Reply to: lombardi@ADMIN.ACES.K12.CT.US
>
>  Hello...I'm new at searching for information via computer and not
>really sure if I'm in the correct address for asking guestions.
>However, if there is any way I can get  some info regarding the
>edibility of "BOLETUS RETIPES" it would be appreciated.A field guide
>describes it as a variety of "BOLETES ORNATIPES" or "GOLDSTALK".
>Thank you for any info that may be available.........Frank
>      
>                          Lombardi@admin.aces.k12.ct.us
>
The following are quotes from the Boletus ornatipes description in Nova 
Scotian Boletes by D.W. Grund and K.A. Harrison (p. 78 & 79) published by 
J. Cramer in 1976 as Band 47 in their Bibliotheca Mycologica series:

"Observations: We agree with Smith and Thiers (p. 330, 1971) that B. 
ornatipes is not a synonym of B. retipes (Berk. & Curt.) Sing. There are 
differences in macrochemical reactions. Singer (p.10, 1947) used a 
"beautiful yellow" reaction of the pileus to KOH as a specific character 
for the identification of B. retipes and used it again in the key to this 
species on p.35 (loc. cit.). He placed B. ornatipes (p.9, loc. cit.) in 
synonymy with B. retipes, but apparently never checked for this reaction 
on the northern forms of this complex. B. ornatipes in Nova Scotia does 
not have this reaction to KOH ...."

"Edibility: Here is a case of confused identities. Snell and Dick report 
B. retipes as edible and of good flavor, but as B. retipes is a southern 
species it seems highly probable that Snell and Dick have reported on B. 
ornatipes. It is strongly recommended that anyone wishing to eat this 
common bolete should use the normal precautions. The taste can be 
distinctly bitter."

Hope this is of some help.
Regards,
KHarrison@fcmr.forestry.ca
 


From owner-mycology@net.bio.net Sun Apr 09 23:00:00 1995
Newsgroups: bionet.mycology
Path: biosci!adam.cc.sunysb.edu!news.sprintlink.net!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!newsspool.doit.wisc.edu!decwrl!tribune.usask.ca!canopus.cc.umanitoba.ca!newsflash.concordia.ca!CC.UMontreal.CA!ophelia.BCH.UMontreal.CA!okellyc
From: okellyc@ophelia.BCH.UMontreal.CA (Charles J. O'Kelly)
Subject: Re: Referring to members of higher taxa
Message-ID: <D6u0Fr.9FL@cc.umontreal.ca>
Sender: news@cc.umontreal.ca (Administration de Cnews)
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Date: Mon, 10 Apr 1995 18:23:49 GMT
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In article <3m0vjt$hus@newstand.syr.edu> jworrall@rodan.syr.edu (James J. Worrall) writes:

>I would be grateful for opinions from mycologists, especially
>systematists, on acceptable ways to refer to members of
>certain higher taxa.  

>For instance, if we believe in a class
>Ascomycetes, most of us get in the habit of using sentences
>like: "Ascomycetes produce asci," as if the class were a plural
>reference to all members, rather than saying,  "Members of the
>Ascomycetes produce asci."

my practice in formal writing is to use the latter form, usually something
like "Fungi that belong to the Xxx have ...".  classes do not have
asci :-).  however, in informal writing and lecturing, it is difficult to 
avoid the shortcut.  

>If we believe instead that the group should be recognized at the
>division level, we have a choice of:
>"Ascomycota produce asci," which sounds bad;
[etc.]
>None of these seem completely satisfactory.  Using the term
>"ascomycetes" to refer to members of the division Ascomycota
>may be a bad habit and is certainly confusing to students.

my opinion - the instructor is teaching two things here.  one is the formal
classification of fungi, the other is the way people in the real world,
or as close to real as academia gets :-), speak and write in the lab.

as far as I'm aware, folk in mycology use "ascomycetes" as the common name
for ascus-producing fungi, regardless of what formal classification is
recognized.  ditto "basidiomycetes", "diatoms", "euglenoids", "amoebae"
(even though this group is polyphyletic), etc.  it's bad luck that the
current common name for the ascomycete clade is identical to one of the
higher taxon names used to describe the clade, or one subset of it, but
if the student is to communicate with colleagues, the term(s) in real use
should be learned, in parallel with the more formal terms and complete with
all the inherent contradictions.  

maybe someone has a less ad-hoc solution?  

good luck.


charley o'kelly
mad protistologist
okellyc@bch.umontreal.ca
okellycj@nh.ngs.net

From owner-mycology@net.bio.net Mon Apr 10 23:00:00 1995
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From: Chris Schadt <gash@u.washington.edu>
Newsgroups: bionet.mycology
Subject: Re: Hi...I'm a clueless newbie!
Date: Mon, 10 Apr 1995 19:04:50 -0700
Organization: University of Washington
Lines: 44
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References: <3lp95r$n9i@crl5.crl.com> <3m04tk$j09@newsbf02.news.aol.com> <3m9qjd$763@india.lm.com>
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On 9 Apr 1995, mspear wrote:

> Commercial Agaricus farmers are just like any other
> farmers.  There are some who use a lot of pesticides.  However the
> successful ones got that way by keeping costs down and quality and
> productivity up.  As in most crops this means minimal pesticides,
> Integrated Pest Management, sanitary practices instead of poisons.
> 
> The average Agaricus operation is around 4 million pounds per year. 
> It's got lot's of employees and has the usual constraints of FIFRA
> regulations, insurance inspections, FDA rules and the like.
> 
> The average shiitake or oyster grower is much smaller, not nearly as
> professional, has much less time in the business, and can "fly under
> the radar".  Pest and disease control without poisons are not as well
> worked out for the exotics.  In short these guys tend to use whatever
> they need to and can get away with it.
> 
> The blanket assertion that Agaricus is pesticide laden and shiitake is
> not probably should be discarded.
> 
>     ---- Mark

Another consideration not adressed here is the fact that Agaricus farmer 
most often use straw and chicken manure purchased from farms over which 
they have little control as to what chemicals are being used.  Being as 
how Agaricus are famed for their accumulation of toxins and metals in 
fruiting bodies I can see how this initial cultivation of compost 
material might be an extremely important step in eliminating toxins from 
the product.

 
-------------------------------------------------------------------------
              /\      Christopher W. Schadt | "I never knew how soothing   
   /\        /||\     5249 17th Ave NE #6   |  trees are--many trees and
  /||\      //||\\ .  Seattle, WA 98105     |  patches of open sunlight,
 //||\\  .  //||\\/|\ (206) 517-5840        |  and tree presences--it is
 //||\\ /|\ //||\\/|\ gash@u.washington.edu |  almost like having another
 //||\\ /|\ //||\\/|\                       |  being."
   ||    |    ||   |  Univ. of Washington   |   -D.H.Lawerence      
-------------------------------------------------------------------------


From owner-mycology@net.bio.net Mon Apr 10 23:00:00 1995
Path: biosci!PRIMENET.COM!henrim
From: henrim@PRIMENET.COM (Henri Morin)
Newsgroups: bionet.mycology
Subject: Re: Help with Boletus retipes? ornatipes?
Date: 10 Apr 1995 17:37:38 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
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At 07:27 PM 4/10/95 GMT, Ken Harrison wrote:
>In article <44496.rarnold@teleport.com>, rarnold@teleport.com says...
>>
>>I received this posting to FUNGUS.  Can you help? 
>>Reply to: lombardi@ADMIN.ACES.K12.CT.US
>>
>>  Hello...I'm new at searching for information via computer and not
>>really sure if I'm in the correct address for asking guestions.
>>However, if there is any way I can get  some info regarding the
>>edibility of "BOLETUS RETIPES" it would be appreciated.A field guide
>>describes it as a variety of "BOLETES ORNATIPES" or "GOLDSTALK".
>>Thank you for any info that may be available.........Frank
>>      
>>                          Lombardi@admin.aces.k12.ct.us
>>
>The following are quotes from the Boletus ornatipes description in Nova 
>Scotian Boletes by D.W. Grund and K.A. Harrison (p. 78 & 79) published by 
>J. Cramer in 1976 as Band 47 in their Bibliotheca Mycologica series:
>
>"Observations: We agree with Smith and Thiers (p. 330, 1971) that B. 
>ornatipes is not a synonym of B. retipes (Berk. & Curt.) Sing. There are 
>differences in macrochemical reactions. Singer (p.10, 1947) used a 
>"beautiful yellow" reaction of the pileus to KOH as a specific character 
>for the identification of B. retipes and used it again in the key to this 
>species on p.35 (loc. cit.). He placed B. ornatipes (p.9, loc. cit.) in 
>synonymy with B. retipes, but apparently never checked for this reaction 
>on the northern forms of this complex. B. ornatipes in Nova Scotia does 
>not have this reaction to KOH ...."
>
>"Edibility: Here is a case of confused identities. Snell and Dick report 
>B. retipes as edible and of good flavor, but as B. retipes is a southern 
>species it seems highly probable that Snell and Dick have reported on B. 
>ornatipes. It is strongly recommended that anyone wishing to eat this 
>common bolete should use the normal precautions. The taste can be 
>distinctly bitter."
>
>Hope this is of some help.
>Regards,
>KHarrison@fcmr.forestry.ca
> 
>In France we collect the King Boletus wich is a gourmet mushroom.
>Heree in the states I found some nices specimenes in the High Sierra
>during fall. They were under pines in cool and shady places.
> My family and myself we cook them in a big fryer with garlic and 
> parsley and this was delicious.Be carefull because soe are not edible
> and have not a good taste.
>
>
>


From owner-mycology@net.bio.net Mon Apr 10 23:00:00 1995
Newsgroups: bionet.mycology
Path: biosci!rutgers!gatech!news-feed-1.peachnet.edu!usenet.eel.ufl.edu!spool.mu.edu!torn!news.bc.net!unixg.ubc.ca!nntp.cs.ubc.ca!news.UVic.CA!news.pfc.forestry.ca!PFC.Forestry.CA!RWINDER
From: rwinder@PFC.Forestry.CA (Richard Winder)
Subject: BC morels
X-Nntp-Posting-Host: pfc.pfc.forestry.ca
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Date: Mon, 10 Apr 1995 18:20:25 GMT
Lines: 9

OK all you West Coast morel trackers:

I just had a report of a find of 18.5 dozen morels on Vancouver Island.
Looks like it will be a good season!	-RSW


  RICHARD WINDER                    Title: Research Scientist
  Canadian Forest Service           Phone: (604) 363-0773
  Victoria, B.C.                    Internet: RWINDER@A1.PFC.Forestry.CA

From owner-mycology@net.bio.net Mon Apr 10 23:00:00 1995
Path: biosci!adam.cc.sunysb.edu!news.sprintlink.net!cs.utexas.edu!news.tamu.edu!baja.pacificrim.net!nwlink.com!ns.compumedia.com!news
From: cffeelvr@compumedia.com
Newsgroups: bionet.mycology
Subject: Looking for the cause of a scent in a closet
Date: 10 Apr 1995 21:38:38 GMT
Organization: Compumedia Internet Services
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X-Newsreader: AIR News 3.X (SPRY, Inc.)

Greetings, 
  I have a question about a certain smell I really like. This scent lives in one of my closets & I 
have often found it in thrift shops on a lot old clothes. I can't really describe what it smells like 
 but almost everyone I know called it "musty". I love it. Everything I store in this particular 
closet gets the scent after about a month or so. I've asked around & some think it's some 
kind of wood mold or yeast or maybe just rotting wood (my hubby thinks the wood is pine)
the smell is similar to that of old books, but much different & more of a 'woodsy' scent. 
Someone once told me they thought it was possibly caused by candida. I would love to find 
something that smells like this to make into my own personal cologne. Any ideas? thanks.
                                                                                    EC



   My home page address: 
    http://www.compumedia.com/~cffeelvr/
   Black Atmosphere's page:
    http://www.compumedia.com/~str8jkt/BlackAtmosphere.html



From owner-mycology@net.bio.net Mon Apr 10 23:00:00 1995
Path: biosci!rutgers!gatech!howland.reston.ans.net!news.cac.psu.edu!news.tc.cornell.edu!travelers.mail.cornell.edu!newstand.syr.edu!kong.syr.edu!griffin
From: griffin@kong.syr.edu (David H. Griffin)
Newsgroups: bionet.mycology
Subject: Re: gene designations in Neurospora-changes?
Date: 11 Apr 1995 19:01:57 GMT
Organization: SUNY ESF, Syracuse, NY
Lines: 20
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Concerning Dan Ebole's proposal for a more standardized genetic 
terminology. As a nonNeurospora mycologist who has struggled to read 
Neurosporology by diverse authors using diverse terminology systems, I'm 
all for it. I think that the Saccharomycetologists have invented an 
excellent system that is easy to follow (wash my mouth out with . . .). 
Other geneticists of other organisms should consider following suit.

                                  
David H. Griffin               
Department of Environmental &
    Forest Biology
350 Illick Hall
College of Environmental         
    Science and Forestry        
One Forestry Drive
Syracuse NY 13210-2788         
e-mail: griffin@mailbox.syr.edu
  
 


From owner-mycology@net.bio.net Mon Apr 10 23:00:00 1995
Path: biosci!VMS1.TAMU.EDU!dje0282
From: dje0282@VMS1.TAMU.EDU (Dan Ebbole)
Newsgroups: bionet.mycology
Subject: re: gene designations in Neurospora-changes?
Date: 11 Apr 1995 11:28:31 -0700
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Thanks Peter for getting this discussion going.  I'll argue with you.

>My personal bias is against major changes in the Neurospora gene
>designations, for several reasons:
>
>1. Changing things to be consistent with yeast seems like buying into the
>idea that yeast is really the only reasonable system (cf. "the one true
>organism"). I would argue that current Neurospora usage is appropriately
>consistent with Aspergillus.
>

Yeast is more widely studied and more familiar to general audiences, even
non-fungal audiences.  A broad range of people must become familiar with
the yeast system of gene designations.  If N. crassa has the same system it
will simply be easier for people to read N. crassa papers, that's all.


>2. The caps/lower case distinction in yeast is between dominant and
>recessive alleles. We could apply the same approach to Neurospora, but this
>ignores heterokaryons, internuclear complementation, different nuclear
>ratios, etc., aspects of Neurospora that make the definition of dominance a
>little more complex.
>
This is a very good point.  

>3. Suddenly changing designations seems like it would create confusion for
>the future, especially for DNA sequences and papers already entered onto
>the databases. Even if we are employing a historical artifact, we can a
>least be consistent about it. If anything, I would suggest dropping the "-"
>in the gene names; I find it personally annoying and I know that some
>find/search programs on the computer don't recognize the dash as a valid
>character (e.g. no distinction seen between the designations cot-1, cot-2
>and cot).
>
>-Peter Margolis

I doubt that many people would be confused, unfortunately.
------------------------
Dr. Daniel Ebbole
dje0282@summa.tamu.edu
Dept. of Plant Pathology and Microbiology
Texas A&M University
College Station, TX 77843-2132


From owner-mycology@net.bio.net Mon Apr 10 23:00:00 1995
Newsgroups: bionet.mycology
Path: biosci!rutgers!gatech!howland.reston.ans.net!pipex!sunic!sunic.sunet.se!news.lth.se!news.lu.se!news
From: Sigvard.Svensson@botmus.lu.se (Sigvard)
Subject: Re: Kambucha??? 
Message-ID: <1995Apr11.174739.19612@nomina.lu.se>
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References: <yarrumD6G4L3.7o7@netcom.com>  
 <Pine.ULT.3.91.950409051145.12033A-100000@lemming.uvm.edu>
Date: Tue, 11 Apr 1995 17:47:39 GMT
Xdisclaimer: No attempt was made to authenticate the sender's name.

In article <Pine.ULT.3.91.950409051145.12033A-100000@lemming.uvm.edu>
Robert Resnik <resnik@lemming.uvm.edu> writes:

> On Mon, 3 Apr 1995, murcohen wrote:
> 
> > Can someone refer me to an article on this fungus and tea and the health 
> > benefits, if any, derived from it?  Please Email to:
> >                                               yarrum@netcom.com
> >                                       thanks....
> > -- 
> >                                              yarrum@netcom.com



I saw a recent, very good, sort of "review" paper by Paul Stamets in
"Mushroom the Journal"  Winter 1994-95, titled "My adventures with the
Blob". Really readable! He is rather sceptic to uncontroled use, since
The "Kombucha tea", a.o. things contains some antibiotic.


Sigvard Svensson, Botanical museum, Ostra Vallgatan 18, S-223 61 Lund,
Sweden
E-mail (Internet): Sigvard.Svensson@botmus.lu.se
Telex: 812 6154 352 SICS Teletex: 2401-812 6154 352=SICS

From owner-mycology@net.bio.net Mon Apr 10 23:00:00 1995
Path: biosci!CMGM.STANFORD.EDU!margolis
From: margolis@CMGM.STANFORD.EDU (Peter Margolis)
Newsgroups: bionet.mycology
Subject: re: gene designations in Neurospora-changes?
Date: 11 Apr 1995 09:38:02 -0700
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My personal bias is against major changes in the Neurospora gene
designations, for several reasons:

1. Changing things to be consistent with yeast seems like buying into the
idea that yeast is really the only reasonable system (cf. "the one true
organism"). I would argue that current Neurospora usage is appropriately
consistent with Aspergillus.

2. The caps/lower case distinction in yeast is between dominant and
recessive alleles. We could apply the same approach to Neurospora, but this
ignores heterokaryons, internuclear complementation, different nuclear
ratios, etc., aspects of Neurospora that make the definition of dominance a
little more complex.

3. Suddenly changing designations seems like it would create confusion for
the future, especially for DNA sequences and papers already entered onto
the databases. Even if we are employing a historical artifact, we can a
least be consistent about it. If anything, I would suggest dropping the "-"
in the gene names; I find it personally annoying and I know that some
find/search programs on the computer don't recognize the dash as a valid
character (e.g. no distinction seen between the designations cot-1, cot-2
and cot).

-Peter Margolis


---------------------------------------------
McBain



From owner-mycology@net.bio.net Mon Apr 10 23:00:00 1995
Path: biosci!agate!howland.reston.ans.net!news.sprintlink.net!news.primenet.com!usenet
From: mikeeve@primenet.com
Newsgroups: bionet.mycology
Subject: Manchurian Mushroom
Date: 11 Apr 1995 16:27:56 GMT
Organization: Primenet
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Manchurian Mushrooms ?
Was recently given article on how to make tea from these mushrooms.  This is supposed to be good as a medicinal supplement . 
Has anyone heard of this?  Can you give me any info on the Manchurian Mushroom?



From owner-mycology@net.bio.net Mon Apr 10 23:00:00 1995
Path: biosci!rutgers!gatech!howland.reston.ans.net!news.sprintlink.net!sundog.tiac.net!grob.tiac.net!user
From: medcom@tiac.net (T.Wicky)
Newsgroups: bionet.mycology
Subject: Pictures of fungi?
Date: 11 Apr 1995 16:20:28 GMT
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Does anyone know where I can get some online pictures (GIF's) of fungi? 
Anything at all would be a great help.  E-mail: medcom@tiac.net(T.Wicky)

From owner-mycology@net.bio.net Mon Apr 10 23:00:00 1995
Path: biosci!rutgers!gatech!swrinde!cs.utexas.edu!not-for-mail
From: gianni@chaos.dominican.edu (Matthew J. Gianni)
Newsgroups: bionet.mycology
Subject: Mycobacterium tuberculosis?
Date: 10 Apr 1995 23:10:31 -0500
Organization: Dominican College of San Rafael
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Hello All,

Excuse me if this turns out to be the wrong kind of question for this 
group, but I've been studying a little bit about tuberculosis, and I'm 
not sure what is meant by the classification of mycobacterium. What are 
the distigushing features of a mycobacterium that set it apart from 
other forms of bacteria? And what, if anything, does it have in common 
with fungi?

TIA for anything that may shed some light on the subject! :)

-----------------
Matthew J. Gianni <gianni@chaos.dominican.edu>
  
Organic chemistry is the chemistry of carbon compounds.
Biochemistry is the study of carbon compounds that crawl.
		-- Mike Adams

From owner-mycology@net.bio.net Mon Apr 10 23:00:00 1995
Path: biosci!agate!news.mindlink.net!news
From: pklym <pklym@griffin.multimedia.edu>
Newsgroups: bionet.mycology
Subject: Re: BC morels
Date: 11 Apr 1995 22:15:49 GMT
Organization: MIND LINK! Communications Corp.
Lines: 15
Message-ID: <3mev2l$met@deep.rsoft.bc.ca>
References: <D6u0A1.LD@news.pfc.forestry.ca>
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I saw some fresh morels for sale at the Granville Island Market this past   
weekend (Apr.8th). They were 1-2" tall, solid medium-brown in color with   
whitish stalks, and looked very fresh. The vendor said they came from the   
Vancouver Lower Mainland area, NOT Vancouver Island. 
 
As I am totally new to Vancouver, I was wondering if anyone could tell me   
if morels can be found in or around the city (or as close as possible).   
If so, when and where ??? Naturally, I'm not after anyone's secret patch,   
but would just like some general guidance. I'm just looking for some to   
eat and am not a commercial picker. 
 
Any assitance would be greatly appreciated. 
 
Thanks,       Paul


From owner-mycology@net.bio.net Mon Apr 10 23:00:00 1995
Path: biosci!ADMIN.OGI.EDU!msachs
From: msachs@ADMIN.OGI.EDU (Matthew Sachs)
Newsgroups: bionet.mycology
Subject: Re: gene designations in fungi
Date: 11 Apr 1995 15:19:34 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
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A general point concerning genetic nomenclature:

Trends in Genetics recently distributed the first TIG Genetic Nomenclature
Guide to subscribers.  It is a compilation of naming conventions and
database resources for organisms from bacteria to humans, and includes the
"model" fungi.

-----------------------------------------------------------
Matthew Sachs
Department of Chemistry, Biochemistry and Molecular Biology
Oregon Graduate Institute of Science and Technology
20000 NW Walker Road
P.O. Box 91000
Portland, OR  97291-1000
503 690-1487 Phone
503 690-1464 Fax
msachs@admin.ogi.edu



From owner-mycology@net.bio.net Tue Apr 11 23:00:00 1995
Path: biosci!rutgers!gatech!howland.reston.ans.net!news.cac.psu.edu!psuvm!hgw1
Organization: Penn State University
Date: Wed, 12 Apr 1995 08:43:17 EDT
From: Heather Treaster <HGW1@psuvm.psu.edu>
Message-ID: <95102.084317HGW1@psuvm.psu.edu>
Newsgroups: bionet.mycology
Subject: Grad. Assistantships Available
Lines: 14

Graduate Assistantships.  The Penn State Plant Pathology Department has
several assistantships available for M.S.  or Ph.D.  students.  Faculty
programs include a diverse range of research in basic and applied plant
pathology, including: field and greenhouse crop pathology, forest
pathology, sustainable agriculture, molecular fungal taxonomy, mushroom
culture and pathology, fungal genetics, epidemiology, virology, plant
responses to environmental stress, air pollution effects to forest trees
and ecosystems, root-specific metabolism, root-pathogen interactions,
host-parasite interactions.  Excellent facilities available for field
work and biochemical/molecular biology research.  Contact Dr. J.E.
Ayers, Graduate Admissions Committee, Plant Pathology Department, 211
Buckhout Laboratory, University Park, PA 16802 (Phone: 814-865-7069;
Fax: 814-863-7217; E-mail: htreaste@psupen.psu.edu).  Affirmative
Action/Equal Opportunity Employer; women and minorities encouraged to
apply.

From owner-mycology@net.bio.net Tue Apr 11 23:00:00 1995
Path: biosci!rutgers!gatech!howland.reston.ans.net!EU.net!sun4nl!oce.nl!ns.via.nl!usenet
From: mushvg@via.nl.net
Newsgroups: bionet.mycology
Subject: Test
Date: Wed, 12 Apr 95 12:30:12 PDT
Organization: Venlo Internet Access, NL
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This is a Test



From owner-mycology@net.bio.net Tue Apr 11 23:00:00 1995
Path: biosci!rutgers!gatech!howland.reston.ans.net!news.sprintlink.net!uunet!in1.uu.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: drzeiger@aol.com (DRZeiger)
Newsgroups: bionet.mycology
Subject: Re: Mycobacterium tuberculosis?
Date: 11 Apr 1995 20:22:25 -0400
Organization: America Online, Inc. (1-800-827-6364)
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Reply-To: drzeiger@aol.com (DRZeiger)
NNTP-Posting-Host: newsbf02.mail.aol.com

Mycobacteria differ from bacteria in their cell wall makeup.  Mycobacteria
have long chain mycolic acids in their cell walls.  These organisms won't
show up on a regular Gram stain.  An acid fast stain is usually needed. 
Another term used for them is acid fast bacilli.  I'm not sure if they are
related to fungi in any way, although there are some fungi that are acid
fast also.

From owner-mycology@net.bio.net Tue Apr 11 23:00:00 1995
Path: biosci!bloom-beacon.mit.edu!grapevine.lcs.mit.edu!uhog.mit.edu!rutgers!gatech!newsjunkie.ans.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: randbio@aol.com (RandBio)
Newsgroups: bionet.mycology
Subject: CYCLOHEXIMIDE SUBSTITUTE???
Date: 12 Apr 1995 06:21:54 -0400
Organization: America Online, Inc. (1-800-827-6364)
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In view of the shortage and high cost of cycloheximide for use in culture
media for isolation of pathogenic fungi (mycobiotic, mycocel, bhi w/
c&c....) There is a need for a suitable substitute.

Saproxin is a qualified substitute.  Saproxin is more effective than
cycloheximide in media used for isolation of yeast.  Inhibition of
C.neoformans is minimal or non-existent with Saproxin.  Some dermatophytes
are inhibited, as with cycloheximide.

Saproxin is available to mycologist for investigation at no cost.  Please
e-mail your request for delivery to recognized medical/research
facilities.

Disclaimer:Randolph Biomedical developed Saproxin.  This is not a sales
offer, but an acknowledgement of the cycloheximide shortage and a possible
solution.



================================================================
     Robert Randolph          I
     Randolph Biomedical   I               .
     1-401-826-1407           I                               
     randbio@aol.com        I
     rand777.ids.net           I

From owner-mycology@net.bio.net Tue Apr 11 23:00:00 1995
Newsgroups: bionet.mycology
Path: biosci!daresbury!trane.uninett.no!sunic!sunic.sunet.se!news.lth.se!news.lu.se!news
From: Sigvard.Svensson@botmus.lu.se (Sigvard)
Subject: Re: Kambucha??? Citation completion 
Message-ID: <1995Apr12.021546.18330@nomina.lu.se>
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Organization: Botanical museum, Lund University, Sweden
References: <yarrumD6G4L3.7o7@netcom.com> 
 <Pine.ULT.3.91.950409051145.12033A-100000@lemming.uvm.edu>  
 <1995Apr11.174739.19612@nomina.lu.se>
Date: Wed, 12 Apr 1995 02:15:46 GMT
Xdisclaimer: No attempt was made to authenticate the sender's name.

Being asked; this is a more complete citation of the above paper,
though not full (I have only read a photocopy):

  Stamets, Paul: "My Adventures with the Blob"; Mushroom the Journal,
  Winter 1994-95, pp. 5-9.


 Sigvard

From owner-mycology@net.bio.net Tue Apr 11 23:00:00 1995
Path: biosci!sunmail.lrz-muenchen.de!uj44211
From: uj44211@sunmail.lrz-muenchen.de (Joerg Kaemper)
Newsgroups: bionet.mycology
Subject: Correction to "Diff.Display"
Date: 12 Apr 1995 12:34:07 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
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Sorry, we did not now that the signs for micro and degree celsius are not
applicable when you post notes at the bionet.
Please substitute =B5 with micro and
=B0 with degree celsius.

Joerg Kaemper
Institute of Genetics, University of Munich
Maria Ward Str. 1a, 80638 Munich, Germany
Phone: 49-89-17919821
Fax: 49-89-17919820



From owner-mycology@net.bio.net Tue Apr 11 23:00:00 1995
Path: biosci!sunmail.lrz-muenchen.de!uj44211
From: uj44211@sunmail.lrz-muenchen.de (Joerg Kaemper)
Newsgroups: bionet.mycology
Subject: Asilomar handout "Diff. Display" (R. Kahmann)
Date: 12 Apr 1995 11:52:11 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
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Differential Display of eukaryotic mRNAs
(Liang and Pardee, Science 257, 967-971)

I. Reverse Transcription of mRNA

20 =B5L Final volume                      =B5L
dH2O                                    X
5X buffer                               4
DTT (0.1M)                              2
dNTP (250=B5M)                            1.6
mRNA                                    0.2 =B5g DNA-free total RNA
T11XY (10=B5M)                            2
______________________________
Total                                   19 =B5L

65=B0C, 5 min - 37=B0C, 70 min - 95=B0C, 5 min - 4=B0C
1 =B5L RTase (20 units) is added 10 min after at 37=B0C.
 At the end of RT spin the rube briefly to collect
 condensation. Set tubes on ice for PCR or store at -20=B0C
 for later use.


II. PCR

20 =B5L Final Volume                             =B5L
dH2O                                           9.2
10X PCR buffer                                  2
dNTP (25 =B5M)                                   1.6
35S-dATP (12.5mCi)                               1
L-primer (10=B5M) (10mer arbitrary primer 50%GC)  2
T11XY (10=B5M)                                    2
RT mix                                          2
Ampli Taq                                      0.2
____________________________
Total                                           20

Mix well by pipetting up and down. Add 25 =B5L mineral oil.
PCR: 94=B0C, 30 sec. - 40=B0C, 2 min - 72=B0C, 30 sec.
for 40 cycles - 72=B0C, 5 min - 4=B0C.
Use 3.5 =B5L of samples plus 2 =B5L of loading dye and incubate
 at 80=B0C for 2 min before loading onto a DNA sequencing gel (6%).

III. SECONDARY PCR:

1.      Cut out band from dried gel. Soak for 10 min in 100 =B5L of H2O.
2.      Boil for 10 min.
3.      Add:
        a.      1/10 vol NaOAc
        b.      50 =B5g glycogen (Boehringer)
        c.      300 =B5L of ethanol
4.      Incubate for 30 min at -80=B0C.
5.      Microfuge and wash with 80% ethanol.
6.      Dissolve in 10 =B5L of H2O.
7.      PCR (as for primary PCR), using:
        a.      5 =B5L of DNA
        b.      20 =B5M dNTPs
        c.      1 =B5M of each primer
        d.      40 =B5L final vol
8.      Run 20 =B5L on a gel and save the remainder for cloning or
reamplification.

-----   use In Vitrogen TA cloning kit or use fragments directly
to probe cDNA or genomic library

Joerg Kaemper
Institute of Genetics, University of Munich
Maria Ward Str. 1a, 80638 Munich, Germany
Phone: 49-89-17919821
=46ax: 49-89-17919820



From owner-mycology@net.bio.net Tue Apr 11 23:00:00 1995
Path: biosci!rutgers!gatech!howland.reston.ans.net!news.sprintlink.net!uunet!in1.uu.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: fitzdc@aol.com (FitzDC)
Newsgroups: bionet.mycology
Subject: Re: Manchurian Mushroom
Date: 12 Apr 1995 16:50:46 -0400
Organization: America Online, Inc. (1-800-827-6364)
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NNTP-Posting-Host: newsbf02.mail.aol.com

Manchurian mushroom (aka Kambucha):

Look up the topics of Kambucha. Also, New Age Journal had an extensive
article(cover story) about three months back. I haven't used it, but you
do make tea, your fungus grows a "baby" (which you are suuposed to give
away) and people are taking it for anything from colds to cancer and aids.

Hope this helps.

                                                                     
--Fitz

From owner-mycology@net.bio.net Tue Apr 11 23:00:00 1995
Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!news.cac.psu.edu!news.tc.cornell.edu!travelers.mail.cornell.edu!newstand.syr.edu!kong.syr.edu!griffin
From: griffin@kong.syr.edu (David H. Griffin)
Newsgroups: bionet.mycology
Subject: gene designations in Neurospora-changes?
Date: 12 Apr 1995 14:12:59 GMT
Organization: SUNY-ESF, Syracuse NY
Lines: 17
Message-ID: <3mgn5b$jsl@newstand.syr.edu>
NNTP-Posting-Host: kong.syr.edu

For further excellent discussion of gene symbols and other terminology 
for fungal genetics, I recommend reading the following:

Yoder, O. C., B. Valent and F. Chumley.  1986.  Genetic nomenclature and 
practice for plant pathogenic fungi.  Phytopathology 76: 383-385.
                                  
David H. Griffin               
Department of Environmental &
    Forest Biology
350 Illick Hall
College of Environmental         
    Science and Forestry        
One Forestry Drive
Syracuse NY 13210-2788         
e-mail: griffin@mailbox.syr.edu
  


From owner-mycology@net.bio.net Tue Apr 11 23:00:00 1995
Path: biosci!rutgers!uwm.edu!msunews!news
From: Chris Wright <wrightc2@studen.msu.edu>
Newsgroups: bionet.mycology
Subject: Re: want to grow 'em
Date: 12 Apr 1995 18:11:33 GMT
Organization: Michigan State University
Lines: 20
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fitzdc@aol.com (FitzDC) wrote:
>
> I live in the southeast and have property with a spring and a creek.
> Hence, high humidity and lots of perpetual ground moisture in parts. There
> is always alot of native fungi around, but I'd like to know if it's
> possible to "seed"(or should I say "spore") for particular types of
> edibles. Also, where would I purchase the stuff to do so?

Yes !  It's possible to inoculate substrates such as wood chips
with specific mushroom spawn.  There is an excellent company in 
Michigan which supplies high quality, prolific mushroom spawns.
Write to:

EASYGROW MUSHROOMS AND COMPOSTING Inc.
P. O. BOX 2353
Farmington Hills,  MI 48333


Chris


From owner-mycology@net.bio.net Wed Apr 12 23:00:00 1995
Path: biosci!RUMAC.UPR.CLU.EDU!C_BETANCOURT
From: C_BETANCOURT@RUMAC.UPR.CLU.EDU
Newsgroups: bionet.mycology
Subject: prueba
Date: 13 Apr 1995 02:33:16 -0700
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This is a test

From owner-mycology@net.bio.net Wed Apr 12 23:00:00 1995
Path: biosci!RUMAC.UPR.CLU.EDU!C_BETANCOURT
From: C_BETANCOURT@RUMAC.UPR.CLU.EDU
Newsgroups: bionet.mycology
Subject: Database
Date: 13 Apr 1995 02:39:16 -0700
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Iwould like information on databases software programs and protocols for fungi.Thanks for your help.Send iformation to my Email
C_betancourt@Rumac.upr.clu.edu

From owner-mycology@net.bio.net Wed Apr 12 23:00:00 1995
Newsgroups: bionet.mycology
Path: biosci!rutgers!gatech!howland.reston.ans.net!torn!news.bc.net!vanbc.wimsey.com!scipio.cyberstore.ca!nntp.cs.ubc.ca!news.UVic.CA!news.pfc.forestry.ca!PFC.Forestry.CA!RWINDER
From: rwinder@PFC.Forestry.CA (Richard Winder)
Subject: Re: Mycobacterium tuberculosis?
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Organization: Forestry Canada (Pacific Forestry Centre)
References: <Pine.A32.3.91.950410205650.18549A-100000@chaos.dominican.edu>,<3mf6g1$4jd@newsbf02.news.aol.com>
Date: Wed, 12 Apr 1995 20:16:54 GMT
Lines: 17

In article <3mf6g1$4jd@newsbf02.news.aol.com>, drzeiger@aol.com (DRZeiger) writes:
>Mycobacteria differ from bacteria in their cell wall makeup.  Mycobacteria
>have long chain mycolic acids in their cell walls.  These organisms won't
>show up on a regular Gram stain.  An acid fast stain is usually needed. 
>Another term used for them is acid fast bacilli.  I'm not sure if they are
>related to fungi in any way, although there are some fungi that are acid
^^^^^^^^^^^^^^^^^
>fast also.

No.  Mycobacterium, Nocardia, and Corynebacterium are all gram positive 
microbes in the Actinomycete line that form mycelia to various degrees-
but they are in the prokaryotic kingdom Monera.  The Fungi constitute a 
different, eukaryotic kingdom.   -RSW

  RICHARD WINDER                    Title: Research Scientist
  Canadian Forest Service           Phone: (604) 363-0773
  Victoria, B.C.                    Internet: RWINDER@A1.PFC.Forestry.CA

From owner-mycology@net.bio.net Wed Apr 12 23:00:00 1995
Newsgroups: bionet.mycology
Path: biosci!rutgers!gatech!howland.reston.ans.net!torn!news.bc.net!vanbc.wimsey.com!unixg.ubc.ca!nntp.cs.ubc.ca!news.UVic.CA!news.pfc.forestry.ca!PFC.Forestry.CA!RWINDER
From: rwinder@PFC.Forestry.CA (Richard Winder)
Subject: Re: Mycobacterium tuberculosis?
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Organization: Forestry Canada (Pacific Forestry Centre)
References: <Pine.A32.3.91.950410205650.18549A-100000@chaos.dominican.edu>
Date: Thu, 13 Apr 1995 16:35:51 GMT
Lines: 48

In article <Pine.A32.3.91.950410205650.18549A-100000@chaos.dominican.edu>, 
gianni@chaos.dominican.edu (Matthew J. Gianni) writes:
>Hello All,
>
>Excuse me if this turns out to be the wrong kind of question for this 
>group, but I've been studying a little bit about tuberculosis, and I'm 
>not sure what is meant by the classification of mycobacterium. What are 
>the distigushing features of a mycobacterium that set it apart from 
>other forms of bacteria? And what, if anything, does it have in common 
>with fungi?

The genus Mycobacterium, which includes M. tuberculosis, the causal agent
of tuberculosis, is distinguished from other bacteria by its acid fastness-
cells treated with a hot phenolic solution of basic fuschin retain the dye
after subsequent treatment with an dilute acid solution.  This genus is a
member of the Actinomycetes, gram-positive bacteria which form mycelia.  In
the case of Mycobacterium, the mycelium is primitive and unstable- a
transitional form in relation to Actinomycetes with more well developed
mycelial morphology.  I would consider these mycelia to be morphologically 
analagous to fungal mycelia, rather than homologous, because we are talking 
about a prokaryotic (nucleus absent) cell versus eukaryotic (nucleii present) 
cells- different taxonomic kingdoms entirely.  In addition, most fungi have a 
cell wall made of chitin, some have cellulose or other polymers.  I think 
there is one bacterium (Sarcinaia ventriculi) with a cellulose cell wall, and 
another (Acetobacter xylinium) which excretes cellulose.  I don't know of any 
with a chitin cell wall.  Mycobacterium, along with Corynebacterium (C. 
diptheriae = diptheria) and Nocardia (N. farcinica = horse glanders) are 
distinguished from other bacteria by a unique cell wall composition.  Their 
cell wall peptidoglycans are type A (cross linked between meso-diaminopemelic
acid and D-alanine).  An arabinose/galcactose polysacharide is bonded to the 
peptidoglycan, and the wall also has a high lipid content which includes
mycolic acid (branched B-hydroxy acids, R1-CH-CH-COOH).  The arabionse
					   |  |
					   OH R2
galactose polysacharide is involved in immunilogical cross-reactivity.  If
you want to know more about *that*- you're in the wrong group!

My source for most of this info is old, so you can probably find more
up-to-date info, but here it is:

Stanier et. al. 1976.  The Microbial World.  Prentice Hall, NY. 871pp.
-RSW



  RICHARD WINDER                    Title: Research Scientist
  Canadian Forest Service           Phone: (604) 363-0773
  Victoria, B.C.                    Internet: RWINDER@A1.PFC.Forestry.CA

From owner-mycology@net.bio.net Wed Apr 12 23:00:00 1995
Path: biosci!rutgers!gatech!usenet.ins.cwru.edu!cleveland.Freenet.Edu!at591
From: at591@cleveland.Freenet.Edu (Lindsey W. Colby)
Newsgroups: bionet.mycology
Subject: Reishi mushrooms on conifer sawdust?
Date: 13 Apr 1995 23:44:22 GMT
Organization: Case Western Reserve University, Cleveland, Ohio (USA)
Lines: 28
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NNTP-Posting-Host: kanga.ins.cwru.edu


	Would anyone out there happen to know if reishi
	(Ganoderma lucidum) can be grown on supplimented
	conifer sawdust?  I understand the closly related
	G. tsugae is usually found on conifers, but not
	so G. lucidum...

	Stamets reports that Reishi can be grown on a
	"wide variety of soft-wood and hard-wood sawdust",
	but no mention of straight soft-wood sawdust and chips.

	I'm thinking of doing a small scale Reishi project,
	but the only industrial wastes up here in the great
	white north (Maine) seem to be generated from pines
	and such...

	On a related note....does anyone know of a machine
	that is designed to generate coarse sawdust from bulk
	wood?   I'm thinking of some sort of chipper/shredder
	that you feed a log into one end and out the other
	comes an endless supply of sawdust!

	Thanks for your help...

	Lindsey Colby
	Bio dept, Colby College
	Waterville, Maine
	lwcolby@host0.colby.edu

From owner-mycology@net.bio.net Wed Apr 12 23:00:00 1995
Path: biosci!biosci!not-for-mail
From: andrewt@r-node.io.org (Andrew Thomas)
Newsgroups: bionet.cellbiol,bionet.general,bionet.genome.chromosomes,bionet.immunology,bionet.microbiology,bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.molec-model,bionet.mycology,bionet.plants,bionet.software,bionet.virology
Subject: SURVEY - Things we'd like to see (commercial)
Date: 13 Apr 1995 14:44:34 -0700
Organization: Sakura Software Inc., Mississauga, Ontario, Canada
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Xref: biosci bionet.cellbiol:2072 bionet.general:14638 bionet.genome.chromosomes:541 bionet.immunology:3823 bionet.microbiology:1994 bionet.molbio.methds-reagnts:27180 bionet.molbio.proteins:4282 bionet.molec-model:320 bionet.mycology:1936 bionet.plants:6388 bionet.software:11785 bionet.virology:2246


I am posting this for a business consulting company.  It is a
questionnaire on the subject of lab techniques, equipment and software
in the biotechnology field, with an eye toward introducing prducts
into those areas where respondents feel there are weaknesses.  This is
your chance to complain about everything from overpriced equipment to
ergonomic disfunction :-).  Please send responses to one of the two
addresses below:

	couper@magi.com
	andrewt@io.org

Thanks in advance,
	Andrew Thomas

-------------------------------------------------------------

                                                April 5, 1995

ADP & Associates
42 Rideau River Lane
Ottawa, Ontario
K1S 0X1

TEL:   (613)526-5609
email: couper@magi.com



We are a business consulting company conducting a market study
on  behalf  of a research and development group interested  in
expanding into biotechnology. Our client is specialized in the
creation  of  new  and  innovative scientific  equipment.  The
object of this survey is to get the direct input of the actual
users  of  the  intended technology. Our client is  constantly
looking  to  develop  products that will simplify  your  work,
save you time, improve safety and reduce your operating costs.

In the questionnaire we have tried to find a balance where the
questions will simultaneously cover all our respondants  areas
of interest and be specific enough for their particular needs.
We  have  also tried to keep it as short as possible.   Should
you  find  that our questionnaire does not adequately  address
your  interests, please feel free to add your comments at  the
end.  All of your comments are valuable to us and all of  your
ideas  have  potential.  Our client is  capable  of  producing
products  with  a varying range of complexity, from  the  most
rudimentary test-tubes to specialized automation hardware  and
computer software. Should your suggestion be a starting  point
for   an   actual   product,   you   will   qualify   for   an
idea/development bonus of $500.00

We  would like to take this opportunity to thank you for  your
valuable  input. If you have any questions or require  further
information  please  feel  free to contact  us  at  the  above
address or telephone number.

If  you would like to be kept informed of our client's product
development, please print your name, address and FAX number at
the bottom of the questionnaire.


Yours truly,
A. Douglas Palmer.

--------------------------------------------------------------

                        Questionnaire

1.   What   are  the  three  most  time  consuming  tasks   or
     procedures carried out in your laboratory?



2.   Is equipment used in performing these tasks?     [Yes/No]
     If so, what?

     If not, what would you like to see made available?

3.   What types of equipment or tools for research, production
     or management would you like to see made available to you
     (not currently available on the market)?
     1.
     2.
     3.
     4.
     5.

     What  improvements (not available on the market) to  your
     current equipment would you like to see? Please describe.


4.   What  types  of  equipment or tools, which  you  are  not
     currently  using, would you purchase if  it  were  priced
     lower?  What type of equipment or tools do you  feel  you
     are paying too much for?


5.   What dangerous activities do you regularly perform? Could
     they be made safer with better tools or equipment?


6.   What  are  some of the more "menial" tasks  performed  in
     your  laboratory?  (tasks which use up valuable personnel
     hours, taking away from research).


7.   Could  your  daily operations be improved by the  use  of
     specialized computer software?                   [Yes/No]
 

     What should this software be capable of achieving? Please
     provide as much detail as you feel appropriate.

     Log book
     Data Manipulation
     Data Analysis
     Database
     Graphics
     Product interfaces
     Simulation and Prediction
     Control and Monitoring of Equipment
     Other


8.   What  are  your fields of expertise? Please  indicate  as
     many as appropriate. (Please be as specific as you can).

     Biochemistry
     Microbiology
     Genetic Research
     Virology
     Plant Research
     Animal Research
     Medical Research
     Pharmaceuticals
     Other.


9.   Is   your   research   primarily   market   oriented   or
     investigative?


10.  What   is   the  size  of  your  laboratory  (number   of
     personnel)?

     1 - 5
     6 - 10
     11 - 15
     16 - 20
     > 20


11.  Which trade publications do you read?
     1.
     2.
     3.
     4
     5


12.  If you have any other comments or suggestions please feel
     free to put them here.




         Name: 

 Work Address: 

        FAX #: 

email address: 
--
Andrew Thomas - President, Sakura Software Inc.
Watch for SCADALisp - Real-time control and UI language for QNX/UNIX
For information, email andrewt@io.org

From owner-mycology@net.bio.net Wed Apr 12 23:00:00 1995
Path: biosci!daresbury!trane.uninett.no!Norway.EU.net!EU.net!news.sprintlink.net!howland.reston.ans.net!vixen.cso.uiuc.edu!news.uoregon.edu!dalbrich1.uoregon.edu!brehm
From: brehm@aaa.uoregon.edu (Matthew T. Brehm)
Newsgroups: bionet.mycology
Subject: eugene.area.morels
Date: Thu, 13 Apr 1995 17:36:24 GMT
Organization: University of Oregon
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Keywords: still looking
X-Newsreader: Trumpet for Windows [Version 1.0 Rev A]

I've been hunting mushrooms for a few seasons and have yet to find any morels. 
 This year it's becoming an obsession, as I venture out a couple times a week 
for that single genus (though along the way I've collected a few meals-worth 
of Dentinum Repandum!).  I'm posting because I'd like some better clues - more 
on WHEN to find them than on WHERE.  Are they simply not fruiting yet around 
Eugene?  Thanks for any info and happy hunting!

From owner-mycology@net.bio.net Wed Apr 12 23:00:00 1995
Path: biosci!rutgers!gatech!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!reuter.cse.ogi.edu!netnews.nwnet.net!news.uidaho.edu!raven.csrv.uidaho.edu!kellogg
From: kellogg@raven.csrv.uidaho.edu (Scott Kellogg)
Newsgroups: bionet.mycology
Subject: Mycobacterium = Procaryote
Date: 13 Apr 1995 03:27:18 GMT
Organization: University of Idaho, Moscow, Idaho
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Mycobacterium is as the name denotes, a bacterium.  Although the 
combining form 'myco' does mean fungus, M. tuberculosis has nothing to 
do with fungi except perhaps for the bacterial colony.  Some of the 
bacteria that are in the mycobacterial order are filamentous but they are 
all procaryotes and thus have nothing to do with fungi.  Mycobacteria 
have waxy coats and thus do not stain like 'true' or eubacteria and 
require a special staining procedure (acid-fasting staining).  See any 
microbiology textbook for more information.

--
===================================================================
Scott T. Kellogg, Dept. of Microbiol., Mol. Biol. & Biochem.
Univ. of Idaho, Moscow, ID 83844-3052  Internet: kellogg@uidaho.edu
Phone: 208-885-6966      Fax: 208-885-6518

From owner-mycology@net.bio.net Thu Apr 13 23:00:00 1995
Path: biosci!rutgers!gatech!howland.reston.ans.net!news.sprintlink.net!uunet!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: drzeiger@aol.com (DRZeiger)
Newsgroups: bionet.mycology
Subject: Re: Mycobacterium tuberculosis?
Date: 13 Apr 1995 20:00:22 -0400
Organization: America Online, Inc. (1-800-827-6364)
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NNTP-Posting-Host: newsbf02.mail.aol.com

>No.  Mycobacterium, Nocardia, and Corynebacterium are all gram positive 
>microbes in the Actinomycete line that form mycelia to various degrees-
>but they are in the prokaryotic kingdom Monera.  The Fungi constitute a 
>different, eukaryotic kingdom.   -RSW

I have had a few microbiology courses and no instuctor has ever explained
that these three are related.  They were always covered separately. 
Nocardia as a fungus, Corynebacterium as bacteria and Mycobacteria on
their own.  Thank you for explaining the connection.  Now I know why the 3
look slightly similar when growing in culture (dry and crumbly).

From owner-mycology@net.bio.net Thu Apr 13 23:00:00 1995
Path: biosci!bloom-beacon.mit.edu!satisfied.apocalypse.org!news2.near.net!news.delphi.com!usenet
From: mikefer@delphi.com
Newsgroups: bionet.mycology
Subject: identification of a polypore
Date: Thu, 13 Apr 95 23:14:46 -0500
Organization: Delphi (info@delphi.com email, 800-695-4005 voice)
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I am trying to identify a stalked polypore with limited reference resources.
Soooo. I thought I'd ask. This was found growing on the ground in a pasture
has a woody stipe attached to the cap on its side in a small indentation. Any
ideas where to start looking at the genus level? Thanks

From owner-mycology@net.bio.net Thu Apr 13 23:00:00 1995
Path: biosci!BCM.TMC.EDU!gsmay
From: gsmay@BCM.TMC.EDU (Gregory S. May)
Newsgroups: bionet.mycology
Subject: Please ignore test
Date: 14 Apr 1995 11:41:17 -0700
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test


From owner-mycology@net.bio.net Thu Apr 13 23:00:00 1995
Path: biosci!rutgers!uwm.edu!newsspool.doit.wisc.edu!news.doit.wisc.edu!facstaff.wisc.edu!tjvolk
From: tjvolk@facstaff.wisc.edu (Tom Volk)
Newsgroups: bionet.mycology
Subject: Re: identification of a polypore
Date: Fri, 14 Apr 1995 13:26:44 GMT
Organization: U.S. Forest Service
Lines: 38
Message-ID: <tjvolk.78.2F8E7813@facstaff.wisc.edu>
References: <5uwcYRm.mikefer@delphi.com>
NNTP-Posting-Host: f180-110.net.wisc.edu

Hi Mike.

In article <5uwcYRm.mikefer@delphi.com> mikefer@delphi.com writes:

>From: mikefer@delphi.com
>Newsgroups: bionet.mycology
>Subject: identification of a polypore
>Date: Thu, 13 Apr 95 23:14:46 -0500
>Organization: Delphi (info@delphi.com email, 800-695-4005 voice)

>I am trying to identify a stalked polypore with limited reference resources.
>Soooo. I thought I'd ask. This was found growing on the ground in a pasture
>has a woody stipe attached to the cap on its side in a small indentation. Any
>ideas where to start looking at the genus level? Thanks

This is certainly not as easy as you might think. Although some polypores 
can be easily identified macroscopically, they are the exception rather than 
the rule.  The days are gone when all polypores could be classified as 
Polyporus or Fomes.  Gilbertson & Ryvarden describe 100 genera of Polypores 
in their 2-volume "North American Polypores"  Modern genera of polypores are 
based largely on microscopic characteristics (monomitic vs dimitic vs. 
trimitic, clamps vs. simple septate, certain kinds of cystidia, etc) and 
whether the fungus causes a white or a brown rot.  Some of the polypores are 
very interesting to look at in the microscope-- I recommend it highly.  
There is also a computerized synoptic key to polypores, using the above 
characteristics, called PolyKey by Adaskaveg and Dunlop. I recommend it very 
highly.
As for your polypore in the pasture, I'm sorry to say have no idea without 
seeing it or knowing additional information about its microscopic 
characteristics. 

Incidentally I am scheduled to gicve a morning workshop on 
introduction to identification of polypores and corticiod (resupinate crust) 
fungi at this year's NAMA foray in Bemidji Minnesota at the end of August.
A beautiful place and  a fun time.

---Tom Volk  Center for Forest Mycology Research, Madison Wisconsin
<tjvolk@facstaff.wisc.edu>

From owner-mycology@net.bio.net Thu Apr 13 23:00:00 1995
Path: biosci!agate!howland.reston.ans.net!newsjunkie.ans.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: pokerholm@aol.com (P Okerholm)
Newsgroups: bionet.mycology
Subject: Re: Reishi mushrooms on conifer sawdust?
Date: 14 Apr 1995 18:57:54 -0400
Organization: America Online, Inc. (1-800-827-6364)
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Sender: root@newsbf02.news.aol.com
Message-ID: <3mmuli$220@newsbf02.news.aol.com>
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Reply-To: pokerholm@aol.com (P Okerholm)
NNTP-Posting-Host: newsbf02.mail.aol.com

Lindsey,
        I too am looking into a wood grinder to produce sawdust. I am
starting a Shiitake farm (and others including Reishi) near you in West
Gardiner. If you are successful in locating or creating such a device
please let me know.  Perhaps we can both benefit from it. I will also let
you know if I am successful.
        I posted a question about sawdust production in the Fungus
newsgroup but I haven't gotten any replies yet. I'll forward them if I get
any.
Regards,
Paul

                Paul Okerholm
                Acorn Farms
                RR 5 Box 269
                West Gardiner, ME 04345

From owner-mycology@net.bio.net Thu Apr 13 23:00:00 1995
Path: biosci!BIO.MIE-U.AC.JP!issei
From: issei@BIO.MIE-U.AC.JP (Issei Kobayashi)
Newsgroups: bionet.mycology
Subject: Help! making protoplasts
Date: 14 Apr 1995 00:12:58 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 24
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <9504140711.AA00017@ShobyoPc2.bio.mie-u.ac.jp>
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Dear All,

We are tring to make protoplasts from Rhizoctonia solani AG2-2. We are using
standard procedure to make protoplast from other AGs (AG 1,4 and 5).
But, the efficiency is very low, so we could get very small no of parotoplasts
in this moment. We are using Novozyme234 as wall degrading enzyme and NaCl or
mannitol for stabilizer. We actually got huge numbers of protoplasts from 
AG4 with this method, but not from AG2-2. What is difference in cell wall 
between AG2-2 and Ag4?
If someone has experience to make protoplasts from AG 2-2 or some information
about differences in cell wall in different AGs, please let me know.

Thanks in advance.



          Issei Kobayashi
          Lab of Plant Pathology, Fac of Bioresources,
          Mie University, Tsu city, Mie, 514, Japan
          TEL: 0592-32-1211(X3559)
          [International: +81-592-32-1211(X3559)]
          FAX: 0592-31-9634
          [International: +81-592-31-9634]
          Email: issei@bio.mie-u.ac.jp

From owner-mycology@net.bio.net Thu Apr 13 23:00:00 1995
Path: biosci!daresbury!trane.uninett.no!Norway.EU.net!EU.net!howland.reston.ans.net!swrinde!cs.utexas.edu!news.sprintlink.net!uunet!nwlink.com!ns.compumedia.com!news
From: cffeelvr@compumedia.com
Newsgroups: bionet.mycology
Subject: I want to make more of my favorite scent......
Date: 14 Apr 1995 08:46:46 GMT
Organization: Compumedia Internet Services
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NNTP-Posting-Host: altair.compumedia.com
X-Newsreader: AIR News 3.X (SPRY, Inc.)

Question: is it a type of fungi that causes a musty odor in a closet? I have one closet that has 
a very distinctive (hard to explain what it smells like....)scent in it that also gets on most stuff, 
esp. clothes that I store in there. It is a musty sort of odor similar yet different  than the scent 
of old books & papers. It is not a" moldy" smell, but a warm, somewhat woodsy scent, kind 
of like wet wood.  I love the scent, which I often find on old furniture & clothing in thrift & 
second hand shops. Someone at an herbal shop I shop at occasionally said that they think it 
sounds like candida & another person told me they think it's the wood rotting or something. 
My husband says he thinks the wood is pine. I have tried to make the scent stronger but do 
not know how. I know nothing about molds & fungi's & am seriously trying to make more of 
the scent.  (cuz it isn't very strong even on clothes that have been stored in there for 
months). the only word I can think of to describe the smell is what some people have called 
it...."musty"....any ideas on how to make more? p.s. I keep the door shut on the closet most 
of the time but would love the scent all over our apt. Anyone know of anything else, maybe 
a particular flower or herb that is 'musty' smelling? thanks a bunch for listening.
                                                                                   EarthChylde



   My home page address: 
    http://www.compumedia.com/~cffeelvr/
   Black Atmosphere's page:
    http://www.compumedia.com/~str8jkt/BlackAtmosphere.html



From owner-mycology@net.bio.net Fri Apr 14 23:00:00 1995
Path: biosci!agate!howland.reston.ans.net!gatech!swrinde!hookup!rover.ucs.ualberta.ca!finzi.ccinet.ab.ca!news@ccinet.ab.ca
From: ksanders@ccinet.ab.ca (Ken Sanderson)
Newsgroups: bionet.mycology
Subject: Re: BC morels
Date: 15 Apr 1995 06:04:41 GMT
Organization: CCI Networks, a division of Corporate Computers Inc.
Lines: 20
Message-ID: <3mnnlq$qpi@finzi.ccinet.ab.ca>
References: <D6u0A1.LD@news.pfc.forestry.ca> <3mev2l$met@deep.rsoft.bc.ca>
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In article <3mev2l$met@deep.rsoft.bc.ca>, pklym <pklym@griffin.multimedia.edu> says:
>
>I saw some fresh morels for sale at the Granville Island Market this past   
>weekend (Apr.8th). They were 1-2" tall, solid medium-brown in color with   
>whitish stalks, and looked very fresh. The vendor said they came from the   
>Vancouver Lower Mainland area, NOT Vancouver Island. 
> 
>As I am totally new to Vancouver, I was wondering if anyone could tell me   
>if morels can be found in or around the city (or as close as possible).   
>If so, when and where ??? Naturally, I'm not after anyone's secret patch,   
>but would just like some general guidance. I'm just looking for some to   
>eat and am not a commercial picker. 
> 
>Any assitance would be greatly appreciated. 
> 
>Thanks,       Paul
>
I would suggest spending sometime wandering around the many green areas 
in vancouver and its surrounding municipalities.


From owner-mycology@net.bio.net Fri Apr 14 23:00:00 1995
Path: biosci!GENOME.GENETICS.UGA.EDU!arnold
From: arnold@GENOME.GENETICS.UGA.EDU
Newsgroups: bionet.mycology
Subject: PHYSICAL MAP OF ASPERGILLUS NIDULANS GENOME
Date: 15 Apr 1995 10:07:27 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 17
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <0098EEBA.46E98272.25@genome.genetics.uga.edu>
Reply-To: arnold@bscr.uga.edu
NNTP-Posting-Host: net.bio.net

Physical maps of the Aspergillus nidulans
genome are available by anonymous ftp from

fungus.genetics.uga.edu


  . - - - - - - - - - - - Jonathan Arnold - - - - - - - - - - - - - - - .
  |                       Dept. of Genetics,                            |
  |                       University of Georgia                         |
  |                       Athens, Georgia 30602                         |
  | Phone:       (706) 542-1449                                         |
  | messages:    (706) 542-9359                                         |
  | FAX:         (706) 542-3910                                         |     
  | Internet:    ARNOLD@BSCR.UGA.EDU                                    |
  . - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - .



From owner-mycology@net.bio.net Sat Apr 15 23:00:00 1995
Path: biosci!bloom-beacon.mit.edu!gatech!news-feed-1.peachnet.edu!usenet.eel.ufl.edu!afn02436
From: afn02436@usenet.freenet.ufl.edu (David H. Thomas)
Newsgroups: bionet.mycology
Subject: Shittake Mushrooms
Date: 16 Apr 1995 23:26:48 GMT
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I am just a farmer interested in Shittake Mushrooms. Can someone explain 
the procedures for growing them, and explain what Mycillium is? A source 
of spores, possibly in the Florida area, would be appreciated!

From owner-mycology@net.bio.net Sat Apr 15 23:00:00 1995
Path: biosci!bloom-beacon.mit.edu!gatech!swrinde!pipex!oleane!jussieu.fr!fdn.fr!uunet!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: laural6000@aol.com (LauraL6000)
Newsgroups: bionet.mycology
Subject: morels in the Catskills
Date: 16 Apr 1995 15:31:41 -0400
Organization: America Online, Inc. (1-800-827-6364)
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NNTP-Posting-Host: newsbf02.mail.aol.com

I'm going to look for morels for the first time this year. Any tips on
their season in the Catskills/Hudson Valley? Habitat? Etc? Thanks!

From owner-mycology@net.bio.net Sat Apr 15 23:00:00 1995
Path: biosci!bloom-beacon.mit.edu!gatech!swrinde!cs.utexas.edu!uunet!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: phildale@aol.com (PhilDale)
Newsgroups: bionet.mycology
Subject: Help regarding agar...
Date: 16 Apr 1995 13:17:44 -0400
Organization: America Online, Inc. (1-800-827-6364)
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Hi, all!

I have read that potato dextrose agar is the best for mushroom
cultivation, and I was wondering if lab agar would work, too?  It says
it's a blend of agar, gelatine, and beef nutrients...will this be
effective?

Thanks

From owner-mycology@net.bio.net Sat Apr 15 23:00:00 1995
Path: biosci!agate!spool.mu.edu!uwm.edu!cs.utexas.edu!news.sprintlink.net!uunet!in1.uu.net!munnari.oz.au!yarrina.connect.com.au!harbinger.cc.monash.edu.au!news.cs.su.oz.au!inferno.mpx.com.au!root
From: Joe Davids <wholerth@mpx.com.au>
Newsgroups: bionet.mycology
Subject: Using Coconut Fibre Dust as a mushroom Casing.
Date: 16 Apr 1995 13:29:43 GMT
Organization: Microplex Pty Ltd
Lines: 11
Message-ID: <3mr647$b7d@inferno.mpx.com.au>
NNTP-Posting-Host: dialup-222.mpx.com.au

Does anyone have any information on the use of Coconut Fibre Dust as 
an alternative to Peat Moss in mushroom casing?

Any information will be greatly appreciated.

I can also supply interested parties with all the technical and
physical information on Coconut Fibre Dust.

Please contact me via e-mail: wholerth@mpx.com.au

Joe Davids. 

From owner-mycology@net.bio.net Sun Apr 16 23:00:00 1995
Newsgroups: bionet.mycology
Path: biosci!rutgers!gatech!swrinde!pipex!sunic!sunic.sunet.se!news.lth.se!news.lu.se!news
From: Sigvard.Svensson@botmus.lu.se (Sigvard)
Subject: Re: Kambucha??? Citation completion 
Message-ID: <1995Apr17.201526.6034@nomina.lu.se>
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Organization: Botanical museum, Lund University, Sweden
References: <yarrumD6G4L3.7o7@netcom.com>  
 <Pine.ULT.3.91.950409051145.12033A-100000@lemming.uvm.edu>   
 <1995Apr11.174739.19612@nomina.lu.se>
Date: Mon, 17 Apr 1995 20:15:26 GMT
Xdisclaimer: No attempt was made to authenticate the sender's name.

Being asked; this is a more complete citation of the above paper,
though not full (I have only read a photocopy):

  Stamets, Paul: "My Adventures with the Blob"; Mushroom the Journal,
  Winter 1994-95, pp. 5-9.


 Sigvard

From owner-mycology@net.bio.net Mon Apr 17 23:00:00 1995
Path: biosci!bloom-beacon.mit.edu!mojo.eng.umd.edu!cs.umd.edu!news.umbc.edu!haven.umd.edu!hecate.umd.edu!yorick.umd.edu!cabirac
From: cabirac@yorick.umd.edu (Daniel M. Cabirac)
Newsgroups: bionet.mycology
Subject: Ag Biotech WWW/Gopher Site
Date: 17 Apr 1995 15:06:37 GMT
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    ***************************************************************
    
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               OF THE NATIONAL AGRICULTURAL LIBRARY (USDA) 
    
         Provides AG BIOTECHNOLOGY INFORMATION via WWW and Gopher 
   
    ***************************************************************

    WWW access:  http://www.inform.umd.edu/EdRes/Topic/AgrEnv/Biotech
    Instructions for our gopher are given below.
	
		    **BIOTECH EDUCATIONAL RESOURCES**        
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From owner-mycology@net.bio.net Mon Apr 17 23:00:00 1995
Path: biosci!bcm!cs.utexas.edu!news.sprintlink.net!noc.netcom.net!usenet.eel.ufl.edu!usenet.cis.ufl.edu!caen!newsxfer.itd.umich.edu!gatech!newsfeed.pitt.edu!hudson.lm.com!news.pop.psu.edu!psuvax1!uwm.edu!newsspool.doit.wisc.edu!decwrl!waikato!comp.vuw.ac.nz!canterbury.ac.nz!cantva!zool029
From: zool029@csc.canterbury.ac.nz (KELVIN DUNCAN)
Newsgroups: bionet.mycology
Subject: Re: Shittake Mushrooms
Date: 18 Apr 95 20:31:12 +1200
Organization: University of Canterbury, Christchurch, New Zealand
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In article <3ms93o$952@huron.eel.ufl.edu>, afn02436@usenet.freenet.ufl.edu (David H. Thomas) writes:
> I am just a farmer interested in Shittake Mushrooms. Can someone explain 
> the procedures for growing them, and explain what Mycillium is? A source 
> of spores, possibly in the Florida area, would be appreciated!

Shiitake are great fun and are tremndous food but they are not easy to grow,
especially in warmer climates such as Floridas.
I would strongly recommend that you attend a course, such as those run by
Stamets in Oregon or Bill Chalmers in Vancouver.  There may be training courses
in your area.  Your local FDA can help with advice.  Or look up recent issues
of one of the trade journals dealing with musrooms (Mushroom World is good).

I could write a book on how to grow Shiitake (i