From owner-mycology@net.bio.net Sat Jul 01 23:00:00 1995 Path: biosci!ADMIN.OGI.EDU!msachs From: msachs@ADMIN.OGI.EDU (Matthew Sachs) Newsgroups: bionet.mycology Subject: Re: Neurospora crassa PCR Date: 2 Jul 1995 11:34:20 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 38 Sender: daemon@net.bio.net Distribution: world Message-ID: <9506301810.AA29292@admin.ogi.edu> NNTP-Posting-Host: net.bio.net >I am having a difficult time PCRing N. crassa and I was wondering if >anyone could direct me to a paper that could guide me on optimal >conditions i.e. buffer, template and primer concentrations, enzyme >amount, temperatures and cycle number. This is a protocol Michael Freitag and I have used successfully for amplification of a 662 nt reporter DNA fragment from plasmids and from a single copy integrant in the Neurospora genome using an Ericomp cycler: In a typical reaction (50 =B5l), 5 ng plasmid DNA or 100 ng genomic DNA template, 0.5 =B5M primers, 400 =B5M of each dNTP, 1X VentPolymerase buffer = (10 mM KCl, 20 mM Tris-HCl [pH 8.8], 10 mM [NH4]2SO4, 0.1% Triton X-100; New England Biolabs, Beverly, MA) and VentPolymerase (1 u) were overlaid with 25 =B5l of light mineral oil (Aldrich, St. Louis, MO) and cycled 35 times through the following temperature profile: 90 s at 94=B0C, 30 s at 50=B0C,= 60 s at 72=B0C. The last extension step was carried out for 5 min. Reactions were cooled on ice and extracted with 60 =B5l chloroform, vortexed and phase= s separated by centrifugation for 5 min at 16,000 xg. One-tenth of the reactions were run on an analytical 0.8% agarose gel. Reactions that yielded discrete bands (662 nt) were directly digested with EcoRI [the fragment had internal EcoRI sites] and used for subcloning into pBS SKII. ----------------------------------------------------------- Matthew Sachs Department of Chemistry, Biochemistry and Molecular Biology Oregon Graduate Institute of Science and Technology 20000 NW Walker Road P.O. Box 91000 Portland, OR 97291-1000 503 690-1487 Phone 503 690-1464 Fax msachs@admin.ogi.edu From owner-mycology@net.bio.net Sat Jul 01 23:00:00 1995 Path: biosci!ihnp4.ucsd.edu!agate!howland.reston.ans.net!swrinde!hookup!news.mcgill.ca!news From: Christian Archambault Newsgroups: bionet.mycology Subject: Need chitinase clone from fungus Date: 2 Jul 1995 04:09:21 GMT Organization: McGill University Computing Centre Lines: 8 Message-ID: <3t565h$cuk@sifon.cc.mcgill.ca> NNTP-Posting-Host: i-09.das.mcgill.ca X-Newsreader: AIR News 3.X (SPRY, Inc.) I would need clones for the chitinase gene from fungus (ideally from basidiomycete) Thanx From owner-mycology@net.bio.net Sat Jul 01 23:00:00 1995 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!prairienet.org!egrunden From: egrunden@prairienet.org (Eric Grunden) Newsgroups: bionet.mycology Subject: What should I expect from a general mycology course? Date: 2 Jul 1995 05:38:46 GMT Organization: University of Illinois at Urbana Lines: 20 Message-ID: <3t5bd6$9m1@vixen.cso.uiuc.edu> Reply-To: egrunden@prairienet.org (Eric Grunden) NNTP-Posting-Host: firefly.prairienet.org This Fall I'll be transferring into the U of I and will be taking a general mycology course. Can someone who's taken such a course (espec. at U/I) give me a general idea about what such a course focuses in on? How will it differ from the fungal sections of a scientific biology course? It involves a lab, should I be honing my knowledge of spawning techniques? Will mycorhizzae be covered in such a course? How about mycoparasites (fungi that lives off of other fungi)? Any info. is appreciated.............................. = eg = -- ******************* The Spirit of Nature, a powerful force, Belongs and returns to its creative source. - Excerpted from The Collective Works of Johnny Pokerface - From owner-mycology@net.bio.net Sun Jul 02 23:00:00 1995 Path: biosci!rutgers!uwm.edu!cs.utexas.edu!news.sprintlink.net!cam.news.pipex.net!pipex!edi.news.pipex.net!pipex!oleane!jussieu.fr!univ-lyon1.fr!ghost.dsi.unimi.it!sirio.cineca.it!gopher From: Marco Floriani Newsgroups: bionet.mycology Subject: Re: Psilocybe Cyanescens Date: 3 Jul 1995 08:24:08 GMT Organization: Cineca Lines: 23 Message-ID: <3t89f8$sa9@sirio.cineca.it> References: NNTP-Posting-Host: sunc27.inf.unitn.it Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; SunOS 5.4 sun4m) To: rmp@rmp.seanet.com X-URL: news:rmp.61.2FEBA8CF@rmp.seanet.com Psilocybe cyanescens is a quite rare species, which is nevertheless described in many books dealing with this genus or even in some more general floras. I'm sure you can find a full description of it in R. Watling and N. Mac Gregory's "British Fungus Flora" vol. 5 (which deals with Strophariaceae and Coprinaceae, genera Stropharia, Hypholoma, Melanotus, Psilocybe, Panaeolus, etc.). This species was found very abundant last fall in Switzerland, where it was growing in the urban parks. Alfredo Riva, a well known Swiss mycologist, has presented a complete macro- and microscopic description of the species in the Swiss Bullettin of Mycology, supplying it with a beautiful color photograph. If I remember correctly, it should be the first issue of 1995, but if you are real- ly interested, please E-mail me and I'll check it out. I hope this can be of help Marco Floriani ________________________________________________________________________________ Marco Floriani Via Vigolana, 8 38057 Pergine Valsugana (TN) Tel : 0461/510450 E-mail : ________________________________________________________________________________ From owner-mycology@net.bio.net Sun Jul 02 23:00:00 1995 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!EU.net!Austria.EU.net!newsfeed.ACO.net!aci.cvut.cz!risc.upol.cz!tudynb05.upol.cz!rac From: rac@risc (Vladislav Raclavsky) Newsgroups: bionet.mycology Subject: Help: Vogel's medium N Date: Fri, 30 Jun 1995 15:08:08 Organization: upol.cz Lines: 19 Message-ID: NNTP-Posting-Host: tudynb05.upol.cz Hi myconetters, I'm sorry for the stupid question, but I would like to test the effect of some chemicals on Neurospora crassa. 90 % of the articles I know use Vogel's medium for Neurospora cultivation, but the original journals cited (Am. Natur. 98: 435-446 and Genet Bull. 13: 42-43) aren't simply available for me (I would have to wait 1 month and more for a copy). So I would greatly appreciate if anybody could e-mail me the Vogel's medium composition. Thanks a lot Vladislav e-mail: rac@risc.upol.cz snail-mail: Vladislav Raclavsky, M.D. Department of Biology Univ. Palacky Sch. Med. Hnevotinska 3 CZ-775 15 Olomouc Czech Republic From owner-mycology@net.bio.net Tue Jul 04 23:00:00 1995 Path: biosci!CC.NTU.EDU.TW!r3603207 From: r3603207@CC.NTU.EDU.TW ("C.C.Chen") Newsgroups: bionet.mycology Subject: Isolation of Geotrichum sp.'s spores Date: 4 Jul 1995 05:59:17 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: I use malt extract agar slant to cultivate Geotrichum. After 3 days of growth, I use 1% triton soln to wash the slant getting the spore suspension. But when I cultivate it in the ME agar again. I find that only 5 spores are germinative. I use LM to detecte it finding that there is many spores cross-link together. How can I getting the spores suspension? And how can I getting the mono-spore ? THANK YOU!!!!!!! From owner-mycology@net.bio.net Tue Jul 04 23:00:00 1995 Path: biosci!CC.NTU.EDU.TW!r3603207 From: r3603207@CC.NTU.EDU.TW ("C.C.Chen") Newsgroups: bionet.mycology Subject: Enzyme Location Date: 4 Jul 1995 05:59:42 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: I want to localize the enzyme of fungi .( Geotrichum ) How can I do it? Can anyone tell me? I want to use HPLC to separate the melanoidins. What kinds of column can I use it ? From owner-mycology@net.bio.net Tue Jul 04 23:00:00 1995 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: Luminescent Mycena Date: 5 Jul 1995 15:53:34 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 42 Sender: root@newsbf02.news.aol.com Message-ID: <3teqju$1j1@newsbf02.news.aol.com> Reply-To: stamets1@aol.com (Stamets 1) NNTP-Posting-Host: newsbf02.mail.aol.com Re: Mycena chlorophos, a green mushroom ??? Perhaps someone can help explain a bizarre phenomenon. I have Mycena chlorophos, which, as the name implies should produce green light. When I grew it out, the light was a distinct iridescent, cool blue light with absolutely no greenish hues. This was witnessed by 4 others. It lit up like a light bulb when the lights were turned out - no waiting for your eyes to adjust. We set up 3 Nikon camers (F-3, N8008, F4) and shot 3 types of film (Kodak Print Gold 400, Tungsten Ektachrome 100, and Kodachrome Professional 64) under various conditions. The Kodakchrome Gold gave the best image - of Green luminescence - while the others also gave partial images (also green). No blue at all! Have any of you had a similar experiences? 1.) Is this an indication of reciprocity failure of the film (15 sec - 3 minute) exposures? Would reciprocity failture show a shift from blue to green ? 2.) Was the species misnamed due to similar experiences - i.e. when the photographs came back, they looked green, hence the name. Might it not be better called have Mycena cyanophos ? Where was this species first published ? A copy of the original article would be gratefully received. 3) Are there other examples or references which might explain this phenomenon? We have posted an excellent photograph of this luminescent mycelium on our Home Page at http://www.halcyon.com/mycomed/fppage.html Check it out and please provide any pertinent comments. Thanks to all. paul stamets From owner-mycology@net.bio.net Tue Jul 04 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!lamarck.sura.net!news.uky.edu!chuck From: Chuck Staben Newsgroups: bionet.mycology Subject: Re: Help: Vogel's medium N Date: 5 Jul 1995 15:28:42 GMT Organization: University of Kentucky Lines: 40 Message-ID: <3teb3a$h9n@service1.uky.edu> References: NNTP-Posting-Host: bs6.mi.uky.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Following is our lab recipe, lifted intact from David Perkins': THIS IS A 50X STOCK (sorry-no subscripts) Dissolve each component before adding the next. Add all to 750 ml water. sodium citrate.2water (125g) OR sodium citrate.5water (150g) KH2PO4 (monobasic potassium phosphate) (250g) NH4NO3 (ammonium nitrate) (100 g) MgSO4.7water (magnesium sulfate heptahydrate) (10g) CaCl2.2water (calcium chloride) (5g) ADD: Trace element solution 5 ml Biotin solution (0.1 mg/ml) 2.5 ml Add water to 1 liter. Add 5 ml CHCl3 as a preservative. Store at room temperature. To make liquid or solid medium, dilute to 1X, add carbon source (1.5% sucrose is typical) Use agar at 1.5-2.0% for solid media. Trace element solution: citric acid 5 g ZnSo4.7H2o 5 g Fe(NH4)2(SO4)2.6H20 1g CuSO4.5H2O 0.25 g MnSO4.1H2O 0.05g H3BO3 0.05 g Na2MO4.2H2O 0.05 g Dissolve in 95 ml water. Add 1 ml chloroform as preservative. Store at room temperature Biotin is dissolved in water and stored frozedn in small aliquots. From owner-mycology@net.bio.net Tue Jul 04 23:00:00 1995 Path: biosci!CYBERGATE.COM!jolivas From: jolivas@CYBERGATE.COM (John L. Olivas) Newsgroups: bionet.mycology Subject: Axenic VAM Culture with spores Date: 5 Jul 1995 16:10:35 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 38 Sender: daemon@net.bio.net Distribution: world Message-ID: <9507052304.AA04924@CyberGate.COM> NNTP-Posting-Host: net.bio.net On April 28, Heidi wrote: >Return-Path: >To: mycology@net.bio.net >From: Heidi-Jayne Hawkins >Subject: Axenic VAM Culture with spores >Date: 28 Apr 1995 16:12:09 GMT >Nntp-Posting-Host: rs60.ws1.uni-hohenheim.de > >Greetings! > >I am working with VAM inoculated plants under sterile conditions. This >requires a sterile inoculum source - ie. spores. I have been isolating >spores (by centrifugation on sucrose), harvesting (by Pasteur pipette >connected to a vacuum or not - under the disection microscope) and >cleaning (by rolling over filter paper) and sterilising (by transferral >to sterilant in a cup sieve under a laminar flow hood) - all these >processes are very tedious and a lot of transferals means that spores >are lost. > >Can anyone with experience of spores advise any other methods or >modifications of the methods above. I would sincerely appreciate this >as each plant inoculated required 500 to 1000 spores; this involves >thus for one experiment large amounts of soil filtrate! > >Thanks. > >Yours sincerely >Heidi > I have information for Heidi, but here address is incorrect. Does anyone have it? John L. Olivas - Phone 209-269-1152 Technical Representative - Office 800-USA-BIO1 BioScientific, Inc. - Fax 209-439-8639 3574 W. Escalon Avenue - Email jolivas@cybergate.com Fresno, California 93711 - From owner-mycology@net.bio.net Tue Jul 04 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!lamarck.sura.net!news.uky.edu!chuck From: Chuck Staben Newsgroups: bionet.mycology Subject: Re: Help: Vogel's medium N Date: 5 Jul 1995 15:27:19 GMT Organization: University of Kentucky Lines: 60 Message-ID: <3teb0n$h9n@service1.uky.edu> References: NNTP-Posting-Host: bs6.mi.uky.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: rac@risc Following is our lab recipe, lifted intact from David Perkins': THIS IS A 50X STOCK Dissolve each component before adding the next. Add all to 750 ml water. sodium citrate.2water (125g) OR sodium citrate.5water (150g) KH2PO4 (monobasic potassium phosphate) (250g) NH4NO3 (ammonium nitrate) (100 g) MgSO4.7water (magnesium sulfate heptahydrate) (10g) CaCl2.2water (calcium chloride) (5g) ADD: Trace element solution 5 ml Biotin solution (0.1 mg/ml) 2.5 ml Add water to 1 liter. Add 5 ml CHCl3 as a preservative. Store at room temperature. To make liquid or solid medium, dilute to 1X, add carbon source (1.5% sucrose is typical) Use agar at 1.5-2.0% for solid media. Trace element solution: citric acid 5 g ZnSo4.7H2o 5 g Fe(NH4)2(SO4)2.6H20 1g CuSO4.5H2O 0.25 g MnSO4.1H2O 0.05g H3BO3 0.05 g Na2MO4.2H2O 0.05 g Dissolve in 95 ml water. Add 1 ml chloroform as preservative. Store at room temperature Biotin is dissolved in water and stored frozedn in small aliquots. rac@risc (Vladislav Raclavsky) wrote: > Hi myconetters, > > I'm sorry for the stupid question, but I would like to test the >effect of some chemicals on Neurospora crassa. 90 % of the articles I >know use Vogel's medium for Neurospora cultivation, but the original journals >cited (Am. Natur. 98: 435-446 and Genet Bull. 13: 42-43) aren't simply >available for me (I would have to wait 1 month and more for a copy). So I >would greatly appreciate if anybody could e-mail me the Vogel's medium >composition. > Thanks a lot > Vladislav >e-mail: rac@risc.upol.cz >snail-mail: >Vladislav Raclavsky, M.D. >Department of Biology >Univ. Palacky Sch. Med. >Hnevotinska 3 >CZ-775 15 Olomouc >Czech Republic From owner-mycology@net.bio.net Wed Jul 05 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!swrinde!sgigate.sgi.com!enews.sgi.com!decwrl!waikato!comp.vuw.ac.nz!canterbury.ac.nz!MANUKA.lincoln.ac.nz!usenet From: Julie Dale Newsgroups: bionet.mycology Subject: VACANCY Date: 6 Jul 1995 03:27:05 GMT Organization: Lincoln University Lines: 3 Message-ID: <3tfl69$2qr@MANUKA.lincoln.ac.nz> NNTP-Posting-Host: pc095227.lincoln.ac.nz Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: dalej@lincoln.ac.nz CURRENT VACANCY From owner-mycology@net.bio.net Wed Jul 05 23:00:00 1995 Newsgroups: bionet.mycology,soc.culture.french,rec.arts.books.marketplace Path: biosci!galaxy.ucr.edu!library.ucla.edu!agate!tcsi.tcs.com!uunet!in1.uu.net!hobbes!asco.london.sco.com!mourlhon From: mourlhon@asco.london.sco.com (Patrick Mourlhon) Subject: French Typographic books Organization: SCO, Inc. Date: Wed, 05 Jul 1995 10:32:57 GMT Message-ID: <1995Jul05.103257.26856@sco.com> References: <3sd33r$l1p@panix.com> <3sl3jk$kmh@srvr1.engin.umich.edu> Sender: news@sco.com (News admin) Lines: 10 Xref: biosci bionet.mycology:2398 soc.culture.french:54821 rec.arts.books.marketplace:14156 Is there anyone that could tell me of a good french book, i could buy about typography ? y a-t-il quelqu'un qui pourrait me donner un nom d'un livre francais concernant des regles typographique ? Merci d'avance. Thank in advance. Patrick From owner-mycology@net.bio.net Wed Jul 05 23:00:00 1995 Path: biosci!CC.NTU.EDU.TW!r3603207 From: r3603207@CC.NTU.EDU.TW ("C.C.Chen") Newsgroups: bionet.mycology Subject: Monospore isolation? Date: 6 Jul 1995 09:08:35 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 6 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Does anyone have experience about manipulation of monospore isolation? How to do monospore isolation? Can anyone tell me? I have culture of Geotricum sp.in malt extract agar slants, and after 3 days of incubation at 30 degree celsius, I used 1% triton solution to obtain the spores. The spores was incubated in malt extract agar but the germination rate was very poor. Did I do anything wrong in the procedure? From owner-mycology@net.bio.net Wed Jul 05 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!cam.news.pipex.net!pipex!dish.news.pipex.net!pipex!oleane!jussieu.fr!univ-lyon1.fr!ghost.dsi.unimi.it!sirio.cineca.it!gopher From: Marco Floriani Newsgroups: bionet.mycology Subject: (no subject) Date: 6 Jul 1995 14:14:24 GMT Organization: Cineca Lines: 31 Message-ID: <3tgr40$226@sirio.cineca.it> NNTP-Posting-Host: sunc16.inf.unitn.it Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; SunOS 5.4 sun4m) X-URL: news:bionet.mycology Some months ago there was a long discussion on this newsgroup about the identity of the fungus that Italians call "porcino". Well, now you can take a look at four magnificent, original Italian "porcini" (Boletus edulis) at the following URL: http://www.inf.unitn.it/~mflorian/fungi/boleedul.html You can also view another dozen of photos of fungi in my personal image archive, which is located at this URL: http://www.inf.unitn.it/~mflorian/mpia.html which is part of my mycological home-page: http://www.inf.unitn.it/~mflorian/mycopage.html Will you take a look at this URLs and let me know what you think about them? Thank you very much Marco ________________________________________________________________________________ Marco Floriani Via Vigolana, 8 38057 Pergine Valsugana (TN) Tel : 0461/510450 E-mail : or ________________________________________________________________________________ From owner-mycology@net.bio.net Wed Jul 05 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!swrinde!dish.news.pipex.net!pipex!oleane!jussieu.fr!unilim.fr!cict.fr!news From: "philippe j. blanc" Newsgroups: bionet.mycology Subject: production of antibiotics by fungus Date: 6 Jul 1995 15:07:53 GMT Lines: 6 Message-ID: <3tgu89$ltf@news.cict.fr> NNTP-Posting-Host: bio1.insa-tlse.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) can somebody tell me if he has already observed the production of citrinin by the fungus monascus which countries authorize the use of the red pigments of monascus in their food thanks mail to blanc@insa-tlse.fr From owner-mycology@net.bio.net Wed Jul 05 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!swrinde!gatech!europa.chnt.gtegsc.com!news.sprintlink.net!howland.reston.ans.net!xlink.net!news.dfn.de!uni-muenster.de!TUD2.UNI-MUENSTER.DE!tudzyns From: tudzyns@uni-muenster.de (Prof. Dr. P. Tudzynski) Newsgroups: bionet.mycology Subject: ECFG3 - 3rd European Congress for Fungal Genetics, Muenster Date: Thu, 6 Jul 1995 12:37:58 GMT Organization: Inst. allg. Botanik Lines: 14 Message-ID: NNTP-Posting-Host: tud2.uni-muenster.de X-Newsreader: Trumpet for Windows [Version 1.0 Rev B final beta #4] ECFG3 - 3rd European Congress for Fungal Genetics, Muenster March 27 - 30, 1996 contact address: Prof. Dr. P. Tudzynski WWU Muenster Inst. f. Botanik Schlossgarten 3 48149 Muenster Fax: 0251 83 3823 Email: tudzyns@uni-muenster.de From owner-mycology@net.bio.net Wed Jul 05 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!spool.mu.edu!agate!ames!waikato!comp.vuw.ac.nz!canterbury.ac.nz!MANUKA.lincoln.ac.nz!usenet From: Julie Dale Newsgroups: bionet.mycology Subject: VACANCIES - POSTDOC AND RESEARCH TECH. - NZ Date: 6 Jul 1995 03:40:58 GMT Organization: Lincoln University Lines: 67 Message-ID: <3tfm0a$2qr@MANUKA.lincoln.ac.nz> NNTP-Posting-Host: pc095227.lincoln.ac.nz Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) PLANT SCIENCE DEPARTMENT POSTDOCTORAL FELLOWSHIP Fungal Molecular Biology and Ecology (Vacancy No. 95/44) An opportunity exists for a Postdoctoral Fellow to become part of the research programme ‘Molecular Ecology of Vegetable Pathogens and their Biological Control Agents’. Research experience in fungal molecular biology is required, as is familiarity with the following techniques; DNA isolation, PCR, agarose and polyacrylamide gel electrophoresis, cloning, sequence analysis and southern analysis. This position has been established for a maximum of three years and will be renumerated at a rate commensurate with qualifications and experience. For Conditions of Appointment and an application form, please contact the Personnel Section. Please quote the relevant vacancy number when making enquiries. Applications close Friday, 21 July 1995. Personnel Section Tel: (03) 325-2811 extn 8575 Fax: (03) 325-3870 Email: Huttonv@lincoln.ac.nz PLANT SCIENCE DEPARTMENT RESEARCH TECHNICIAN Plant Pathology (Vacancy No. 95/45) A further position is available for a Research Technician. The appointee should have research experience in mycology or plant pathology and should be familiar with the following techniques; microbial culturing, fungal isolation and inoculation techniques, DNA isolation, PCR, gel electrophoresis, cloning and sequence analysis. The successful applicant will act as a Research Technician for the Plant Pathology Research Group. This position is for a maximum of two years and the salary will be based on the Lincoln University salary scales for Technicians 1 up to a maximum of $22,200 per annum. For Conditions of Appointment and an application form, please contact the Personnel Section. Please quote the relevant vacancy number when making enquiries. Applications close Friday, 21 July 1995. Personnel Section Tel: (03) 325-2811 extn 8575 Fax: (03) 325-3870 Email: Huttonv@lincoln.ac.nz Lincoln University is committed to a policy of equal opportunity in education and employment. From owner-mycology@net.bio.net Wed Jul 05 23:00:00 1995 Path: biosci!rutgers!gatech!bloom-beacon.mit.edu!news.starnet.net!wupost!waikato!comp.vuw.ac.nz!canterbury.ac.nz!MANUKA.lincoln.ac.nz!usenet From: Julie Dale Newsgroups: bionet.mycology Subject: vacancy - POSTDOCTORAL FELLOWSHIP Date: 6 Jul 1995 03:38:37 GMT Organization: Lincoln University Lines: 37 Message-ID: <3tflrt$2qr@MANUKA.lincoln.ac.nz> NNTP-Posting-Host: pc095227.lincoln.ac.nz Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) PLANT SCIENCE DEPARTMENT POSTDOCTORAL FELLOWSHIP Fungal Molecular Biology and Ecology (Vacancy No. 95/44) An opportunity exists for a Postdoctoral Fellow to become part of the research programme ‘Molecular Ecology of Vegetable Pathogens and their Biological Control Agents’. Research experience in fungal molecular biology is required, as is familiarity with the following techniques; DNA isolation, PCR, agarose and polyacrylamide gel electrophoresis, cloning, sequence analysis and southern analysis. This position has been established for a maximum of three years and will be renumerated at a rate commensurate with qualifications and experience. For Conditions of Appointment and an application form, please contact the Personnel Section. Please quote the relevant vacancy number when making enquiries. Applications close Friday, 21 July 1995. Personnel Section Tel: (03) 325-2811 extn 8575 Fax: (03) 325-3870 Email: Huttonv@lincoln.ac.nz Lincoln University is committed to a policy of equal opportunity in education and employment. From owner-mycology@net.bio.net Wed Jul 05 23:00:00 1995 Path: biosci!rutgers!gatech!bloom-beacon.mit.edu!news.starnet.net!wupost!waikato!comp.vuw.ac.nz!canterbury.ac.nz!MANUKA.lincoln.ac.nz!usenet From: Julie Dale Newsgroups: bionet.mycology Subject: RESEARCH TECHNICIAN VACANCY - NEW ZEALAND Date: 6 Jul 1995 03:36:12 GMT Organization: Lincoln University Lines: 35 Message-ID: <3tflnc$2qr@MANUKA.lincoln.ac.nz> NNTP-Posting-Host: pc095227.lincoln.ac.nz Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: stewarta PLANT SCIENCE DEPARTMENT RESEARCH TECHNICIAN Plant Pathology (Vacancy No. 95/45) A further position is available for a Research Technician. The appointee should have research experience in mycology or plant pathology and should be familiar with the following techniques; microbial culturing, fungal isolation and inoculation techniques, DNA isolation, PCR, gel electrophoresis, cloning and sequence analysis. The successful applicant will act as a Research Technician for the Plant Pathology Research Group. This position is for a maximum of two years and the salary will be based on the Lincoln University salary scales for Technicians 1 up to a maximum of $22,200 per annum. For Conditions of Appointment and an application form, please contact the Personnel Section. Please quote the relevant vacancy number when making enquiries. Applications close Friday, 21 July 1995. Personnel Section Tel: (03) 325-2811 extn 8575 Fax: (03) 325-3870 Email: Huttonv@lincoln.ac.nz Lincoln University is committed to a policy of equal opportunity in education and employment. From owner-mycology@net.bio.net Wed Jul 05 23:00:00 1995 Path: biosci!agate!ames!waikato!comp.vuw.ac.nz!canterbury.ac.nz!MANUKA.lincoln.ac.nz!usenet From: Julie Dale Newsgroups: bionet.mycology Subject: POSTDOCTORAL VACANCY - NEW ZEALAND Date: 6 Jul 1995 03:32:43 GMT Organization: Lincoln University Lines: 36 Message-ID: <3tflgr$2qr@MANUKA.lincoln.ac.nz> NNTP-Posting-Host: pc095227.lincoln.ac.nz Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: STEWARTA PLANT SCIENCE DEPARTMENT POSTDOCTORAL FELLOWSHIP Fungal Molecular Biology and Ecology (Vacancy No. 95/44) An opportunity exists for a Postdoctoral Fellow to become part of the research programme ‘Molecular Ecology of Vegetable Pathogens and their Biological Control Agents’. Research experience in fungal molecular biology is required, as is familiarity with the following techniques; DNA isolation, PCR, agarose and polyacrylamide gel electrophoresis, cloning, sequence analysis and southern analysis. This position has been established for a maximum of three years and will be renumerated at a rate commensurate with qualifications and experience. For Conditions of Appointment and an application form, please contact the Personnel Section. Please quote the relevant vacancy number when making enquiries. Applications close Friday, 21 July 1995. Personnel Section Tel: (03) 325-2811 extn 8575 Fax: (03) 325-3870 Email: Huttonv@lincoln.ac.nz Lincoln University is committed to a policy of equal opportunity in education and employment. From owner-mycology@net.bio.net Wed Jul 05 23:00:00 1995 Path: biosci!rutgers!gatech!news.sprintlink.net!newsfeed.internetmci.com!news.compuserve.com!newsmaster From: <74752.1434@compuserve.com> Newsgroups: bionet.mycology Subject: Nashua, NH Myco Contact? Date: 6 Jul 1995 03:28:10 GMT Organization: CompuServe Incorporated Lines: 7 Message-ID: <3tfl8a$a32@dub-news-svc-4.compuserve.com> NNTP-Posting-Host: ad04-033.compuserve.com Content-Type: text/plain Content-length: 156 X-Newsreader: AIR Mosaic (16-bit) version 1.00.198.07 I am interested in joining the Nashua, NH Mycological group. Can anyone give me a contact name? I have no idea where or when they meet. Thanks, Mike From owner-mycology@net.bio.net Wed Jul 05 23:00:00 1995 Path: biosci!rutgers!gatech!europa.chnt.gtegsc.com!newsfeed.internetmci.com!news.compuserve.com!newsmaster From: <74752.1434@compuserve.com> Newsgroups: bionet.mycology Subject: Nashua, NH Myco Date: 6 Jul 1995 03:25:53 GMT Organization: CompuServe Incorporated Lines: 2 Message-ID: <3tfl41$a32@dub-news-svc-4.compuserve.com> NNTP-Posting-Host: ad04-033.compuserve.com Content-Type: text/plain Content-length: 0 X-Newsreader: AIR Mosaic (16-bit) version 1.00.198.07 From owner-mycology@net.bio.net Wed Jul 05 23:00:00 1995 Path: biosci!KUHUB.CC.UKANS.EDU!fgsc From: fgsc@KUHUB.CC.UKANS.EDU (Fungal Genetics Stock Center) Newsgroups: bionet.mycology Subject: 1995 Fungal Genetics Newsletter Date: 6 Jul 1995 14:08:37 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HSK0QCX14Y8XH3QR@KUHUB.CC.UKANS.EDU> NNTP-Posting-Host: net.bio.net Attention FGN Readers! The 1995 Newsletter will go to the printer in the next few days. We hope it will be shipped to readers within two weeks. Meanwhile all the articles and bibliographies for this year can be read at the FGSC WWW site: http://www.kumc.edu/research/fgsc/main.html The only items excluded are the sheets of updated RFLP mapping data from Bob Metzenberg (they don't fit into HTML very well) and stocks added to the collection in the last year (they should already be included in catalogue files). As always, let us know if you want to subscribe to the newsletter or have any questions about it or the stock center. Craig Wilson From owner-mycology@net.bio.net Wed Jul 05 23:00:00 1995 Path: biosci!PLANTPATH.PP.KSU.EDU!JFL From: JFL@PLANTPATH.PP.KSU.EDU ("John F. Leslie") Newsgroups: bionet.mycology Subject: Postdoctoral position Date: 6 Jul 1995 11:57:56 -0700 Organization: Plant Pathology Lines: 47 Sender: daemon@net.bio.net Distribution: world Message-ID: <8FFBBA3763@plantpath.pp.ksu.edu> NNTP-Posting-Host: net.bio.net Position Announcement Title: Research Associate (Postdoctoral) Location: Kansas State University Department of Plant Pathology Throckmorton Plant Sciences Center Manhattan, Kansas, USA Appointment: Full-time, 12-month position, with annual reappoint- ment contingent upon continued funding and satisfac- tory performance. Position Available: 1 October 1995 Responsibilities: Conduct research in genetics of Fusarium monili- forme using appropriate classical, molecular or pop- ulation genetic approaches and designed in collabora- tion with the principal investigator. Potential topics include vegetative compatibility, fumonisin biosynthesis, construction of near-isogenic lines, spore-killer, mating-type, and genetic variation in field populations. Qualifications: Ph.D. in genetics, microbiology, plant pathology or related field. Experience with fungal/microbial genetics and/or molecular biology strongly preferred. Salary: $22,000-24,000 per year plus standard university benefits (health and life insurance, vacation and retirement). Applications: Send application, including transcript(s) and resume, and have three letters of reference sent to: Dr. John F. Leslie Department of Plant Pathology 4002 Throckmorton Plant Sciences Center Kansas State University Manhattan, Kansas 66506-5502 USA phone: 913-532-1363 fax: 913-532-2414 e-mail: JFL@PlantPath.pp.KSU.edu Application Deadline: 15 September 1995 Kansas State University is an Equal Opportunity/Affirmative Action Employer. Women and minorities are encouraged to apply. From owner-mycology@net.bio.net Thu Jul 06 23:00:00 1995 Path: biosci!GEMINI.OSCS.MONTANA.EDU!umbjc From: umbjc@GEMINI.OSCS.MONTANA.EDU (Jimmy Cutler) Newsgroups: bionet.mycology Subject: (none) Date: 7 Jul 1995 13:05:37 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net subscribe mycology Jimmy Cutler NNTP-Posting-Host: net.bio.net ANNOUNCEMENT: IMMEDIATE OPENING FOR A GRADUATE (Ph.D.) STUDENT Montana State University has an opening for a graduate student in their Medical Mycology Predoctoral Training Program (MMPTP) which is funded, in part, by the National Institute of Infectious Diseases (NIAID). The intent of the MMPTP is to provide support and training for graduate students who wish to pursue a Ph.D. with research and training emphasis in medical mycology. The number of serious fungal infections has increased dramatically over the past decade and the number is expected to continue to increase. People who develop immmunological deficiencies are especially at risk. The deficiencies may occur because of infectious disease such as AIDS, or because of modern medical practices such as organ transplants and anti-cancer treatments. Eleven research faculty from four departments at MSU will serve as Ph.D. dissertation advisors for trainees in the MMPTP. Clinical laboratory training opportunities, medical mycology courses, workshops and travel support to national scientific meetings are also available through the Program. The graduate students (trainees) will receive an annual stipend of $14,000 and fee waivers. We currently are accepting applications for this coming academic year. The following minimum criteria are required to be considered for admission into the Program: US citizenship; baccalaureate degree in a biological or physical science; grade point average of 3.0 or better in science courses; graduate record exam (GRE). Women and minorities are especially encouraged to apply. Inquires about the Program should be directed to : Jim E. Cutler, Ph.D., Director, MMPTP, Department of Microbiology, Montana State University, Bozeman, MT 59717 (tel: 406-994-2373; FAX:406-994-4926; e-mail: umbjc@gemini.oscs.montana.edu) From owner-mycology@net.bio.net Thu Jul 06 23:00:00 1995 Path: biosci!UNIX1.SNCC.LSU.EDU!esiegel From: esiegel@UNIX1.SNCC.LSU.EDU (Eric Robb Siegel) Newsgroups: bionet.mycology Subject: Re: Help: Vogel's medium N Date: 7 Jul 1995 16:44:27 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <9507072342.AA15360@unix1.sncc.lsu.edu> NNTP-Posting-Host: net.bio.net The Vogel's medium N is also given in a lengthy article by Davis and DeSerre (I think that's the proper spelling) on Neurospora Genetics which appeared in Methods in Enzymology circa 1975. The volume also has articles on yeast genetics and Bacillus genetics. I don't have the Vogel's medium N recipe in front of me, but I use it for growing Fusarium, partly because the recipe is for a 50X stock solution. It sure beats weighing out six or seven mineral salts every time I want to whip up a liter of medium. From owner-mycology@net.bio.net Thu Jul 06 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!rhf.bradley.edu!sunca.ncaur.gov!usenet From: () Newsgroups: bionet.mycology Subject: Re: Monospore isolation? Date: 7 Jul 1995 22:00:07 GMT Organization: USDA:NCAUR:Computer Applications Lines: 21 Distribution: world Message-ID: <3tkap7$q1m@sunca.ncaur.gov> References: NNTP-Posting-Host: novix.ncaur.gov Mime-Version: 1.0 X-Newsreader: Firefox WinVN 1.02 In article , r3603207@CC.NTU.EDU.TW says... > > Does anyone have experience about manipulation of monospore isolation? >How to do monospore isolation? Can anyone tell me? > I have culture of Geotricum sp.in malt extract agar slants, and after 3 >days of incubation at 30 degree celsius, I used 1% triton solution to obtain >the spores. The spores was incubated in malt extract agar but the germination >rate was very poor. Did I do anything wrong in the procedure? I would suggest you try your isolation with a lower concentration of triton. I have used 0.0001 % and have been sucessful with Aspergillus fumigatus. Best of luck, Tim Dvorak From owner-mycology@net.bio.net Fri Jul 07 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!news.bluesky.net!solaris.cc.vt.edu!swiss.ans.net!prodigy.com!usenet From: VPGH51A@prodigy.com (Jim Berlstein) Newsgroups: bionet.mycology Subject: Re: Amanita phalloides - not in my back yard! Date: 8 Jul 1995 15:44:23 GMT Organization: Prodigy Services Company 1-800-PRODIGY Lines: 16 Distribution: world Message-ID: <3tm94n$18es@usenetw1.news.prodigy.com> References: <3tkuho$7tt@newsbf02.news.aol.com> NNTP-Posting-Host: inugap4.news.prodigy.com X-Newsreader: Version 1.2 Since this is a mycorhizal fungus (symbiotic), You might consider the extreme measure of killing the tree, or trees, that the fungus coexists with. If they fruit in a circular area around one particular tree, it should not be too hard to figure out which tree to kill. I think they usually associate with evergreens in this country, although some amanitias seem to associate with different trees in different areas. The man who ID'd the fungus might know. Best of luck, - JIM BERLSTEIN VPGH51A@prodigy.com From owner-mycology@net.bio.net Fri Jul 07 23:00:00 1995 Path: biosci!agate!overload.lbl.gov!emf.emf.net!gatech!newsfeed.pitt.edu!godot.cc.duq.edu!news.duke.edu!usenet From: "Evan L. Cooper" Newsgroups: bionet.mycology Subject: Chanterelle or Jack O'Lantern? Date: 8 Jul 1995 15:23:11 GMT Organization: Duke University / Chem. Dept. Lines: 23 Message-ID: <3tm7sv$s69@news.duke.edu> NNTP-Posting-Host: async32.async.duke.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: elcooper@chem.duke.edu I'm a VERY amateur mycologist equipped with an Audubon field guide to mushrooms. June has been a record rainfall month here in Durham, NC. We live near a forest with many oak trees in our yard. Put it all together and we have many mushrooms growing right now. Great fun! I've identified (?) some boletes, bitter, yellow-cracked and old man of the woods, in latin, Tylopilus fellus, Boletus subtomentosus and Strobilomyces floccopus. There are a couple of Amanitas I'm not sure about. Finally, what about the Chantarelle or Jack O'Lantern question. I'm pretty sure I've got one or the other. How can I tell? Audubon says that Jack, Omphalotus olearius, gives off an eerie gree glow from its gills. Well, I ran straight inside to a closet to check this out, but I didn't see anything. :( Imagine my disappointment. Of course if they're Chanterelles maybe I don't need to be sad. :) Thanks in advance, Evan L. Cooper (elcooper@chem.duke.edu) From owner-mycology@net.bio.net Fri Jul 07 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!news.starnet.net!wupost!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: skikider@aol.com (Skikider) Newsgroups: bionet.mycology Subject: Re: Plastic Bags/Amycel? Date: 8 Jul 1995 01:42:44 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 12 Sender: root@newsbf02.news.aol.com Message-ID: <3tl5sk$acj@newsbf02.news.aol.com> References: <3snf66$g1h@ixnews3.ix.netcom.com> Reply-To: skikider@aol.com (Skikider) NNTP-Posting-Host: newsbf02.mail.aol.com The contact below sell bags of all sizes and shapes and have built in Microporus Filters so no collar and sponge is needed. Unicorn Lou Hsu P.O. Box 785 Water Balley, MS 38965 Ph#:601/675-8000..or..800/888-0811 Fax#:601/675-8800 or. Unicornbag@aol.com Ray Hatch From owner-mycology@net.bio.net Fri Jul 07 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!spool.mu.edu!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: whoff26753@aol.com (WHoff26753) Newsgroups: bionet.mycology Subject: Amanita phalloides - not in my back yard! Date: 7 Jul 1995 23:37:28 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 19 Sender: root@newsbf02.news.aol.com Message-ID: <3tkuho$7tt@newsbf02.news.aol.com> Reply-To: whoff26753@aol.com (WHoff26753) NNTP-Posting-Host: newsbf02.mail.aol.com We live in Mill Valley, California, and have had a dog poisoned by Amanita phalloides from our back yard. We have dug up numerous fruiting bodies under a single large oak tree directly behind our kitchen. These have been confirmed as A. phalloides by a Dr. Robert West, who teaches mycology at College of Marin. Interestingly, although it is apparently extremely unusual, these fruits have been recovered since Christmas day, up to this week (July 1995). We are looking for advice regarding eradication of these mushrooms, which appears to be extremely difficult. A landscaper has suggested using a heavy sheeting over the top of the yard which will allow water through but will not allow growth upwards except where holes are cut for specific plants, but I am concerned that this may in fact enhance the growth of the mushrooms. If you have any advice, or can refer us to another source of information, please post a message on the newsgroup or e-mail to CleftMD@aol.com. Thanks Bill Hoffman and Teresa Holt, Mill Valley, CA From owner-mycology@net.bio.net Fri Jul 07 23:00:00 1995 Path: biosci!rutgers!uwm.edu!reuter.cse.ogi.edu!usenet From: Matthew Sachs Newsgroups: bionet.mycology Subject: Re: Help: Vogel's medium N Date: 7 Jul 1995 23:55:43 GMT Organization: Oregon Graduate Institute - Computer Science & Engineering Lines: 29 Message-ID: <3tkhhv$k3f@reuter.cse.ogi.edu> References: <9507072342.AA15360@unix1.sncc.lsu.edu> NNTP-Posting-Host: fungus.cbs.ogi.edu esiegel@UNIX1.SNCC.LSU.EDU (Eric Robb Siegel) wrote: > > > > The Vogel's medium N is also given in a lengthy article by Davis and DeSerre > (I think that's the proper spelling) on Neurospora Genetics which appeared in > Methods in Enzymology circa 1975. The volume also has articles on yeast > genetics and Bacillus genetics. > > I don't have the Vogel's medium N recipe in front of me, but I use it for > growing Fusarium, partly because the recipe is for a 50X stock solution. It > sure beats weighing out six or seven mineral salts every time I want to whip up a liter of medium. The reference for this article: Davis, R. H., and de Serres, F. J. (1970). Genetic and microbiological research techniques for Neurospora crassa. Methods. Enzymol. 27A, 79-143. ----------------------------------------------------------- Matthew Sachs Department of Chemistry, Biochemistry and Molecular Biology Oregon Graduate Institute of Science and Technology 20000 NW Walker Road P.O. Box 91000 Portland, OR 97291-1000 503 690-1487 Phone 503 690-1464 Fax msachs@admin.ogi.edu From owner-mycology@net.bio.net Fri Jul 07 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!vixen.cso.uiuc.edu!news.uoregon.edu!gaia.ucs.orst.edu!news.PEAK.ORG!microbe From: microbe@PEAK.ORG (Steven Carpenter) Newsgroups: bionet.mycology Subject: Re: Chanterelle or Jack O'Lantern? Date: 8 Jul 1995 20:19:03 GMT Organization: CS Outreach Services, Oregon State University, Corvallis, OR, USA Lines: 3 Message-ID: <3tmp7n$8hq@odo.PEAK.ORG> References: <3tm7sv$s69@news.duke.edu> NNTP-Posting-Host: kira X-Newsreader: TIN [version 1.2 PL2] We tried this many years ago, and found that in an extremely dark room, it took about 10 min. before our eyes were able to see the bioluminescence. Then it was truely marvelous to behold. From owner-mycology@net.bio.net Fri Jul 07 23:00:00 1995 Path: biosci!TELEPORT.COM!lorelei From: lorelei@TELEPORT.COM (Lorelei Norvell) Newsgroups: bionet.mycology Subject: Re: Amanita phalloides - not in my back yard! Date: 8 Jul 1995 12:54:54 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <3tkuho$7tt@newsbf02.news.aol.com> NNTP-Posting-Host: net.bio.net Yes, Amanita phalloides is mycorrhizal, but not with evergreens. We think it might have been introduced into this country from Europe around the turn of the century when European chestnuts were brought in. To my knowledge it has been found with filberts, oaks, and chestnuts (probably beeches as well). If you are truly concerned, you might contemplate removing the tree or trees with which it might be associated. Unfortunately roots do not necessarily extend evenly in all directions from the symbiont partner ... and the fungal partner can often be found growing an unusual distance away from its "host". Lorelei Norvell From owner-mycology@net.bio.net Sat Jul 08 23:00:00 1995 Path: biosci!TELEPORT.COM!lorelei From: lorelei@TELEPORT.COM (Lorelei Norvell) Newsgroups: bionet.mycology Subject: DNA from herbarium specimens Date: 9 Jul 1995 15:25:44 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net I have been successful in extracting and amplifying nDNA (ITS1 + 5.8srRNA + ITS2) from over 100 different dried herbarium specimens. However, I have found that the older the DNA, the greater problems I have encountered. Unfortunately it is imperative I extract DNA from type specimens collected from 1910 to 1970. While I have had some success with the 1970 and 1956 collections, I am encountering problems with degraded DNA. I would welcome advice with respect to [1] Whether drying the specimens again before extraction would increase success or further degrade the DNA (drying with no heat). [2] Another procedure besides the hot CTAB procedure I have used [3] Any other preparation advice. Thanks. Lorelei Norvell University of Washington Department of Botany From owner-mycology@net.bio.net Sat Jul 08 23:00:00 1995 Path: biosci!agate!newsxfer.itd.umich.edu!gatech!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: brianmc@aol.com (BrianMc) Newsgroups: bionet.mycology Subject: Re: Chanterelle or Jack O'Lantern? Date: 9 Jul 1995 15:26:11 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 19 Sender: root@newsbf02.news.aol.com Message-ID: <3tpagj$9jf@newsbf02.news.aol.com> References: <3tm7sv$s69@news.duke.edu> Reply-To: brianmc@aol.com (BrianMc) NNTP-Posting-Host: newsbf02.mail.aol.com The most common mistake beginning amateurs make is an overreliance on a single feature in identifying a mushroom. For the Chantrelle, you want to look for a combination of features. First, are the widely spaced, blunt, fold-like ridges in place of the narrow, close gills found on the Jack-O-Lantern. Second, the odor, which is faint, or mildly fruity (like apricots). The Jack-O-Lantern has a disagreeable odor. The gills on the Jack-O-Lantern are differently colored than the cap. Under a microscope, the differences are startling. Cantherellus has six-spored, and sometimes eve eight-spored basidia (fertile cells) Omphalotus is more typically four-spored. Most amateurs, however, have neither the time, nor the inclination to do microscopy (what a shame!). Keep a careful eye on the mushroom's habitat. Habitat often offers important clues as to the nature of the mushroom. ---------------------------------------------------------- | Brian McNett e-mail: | Editor: MycoInfo Submissions: | Promoting Mycology in the Online Community From owner-mycology@net.bio.net Sat Jul 08 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!usc!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: whoff26753@aol.com (WHoff26753) Newsgroups: bionet.mycology Subject: Re: Amanita phalloides - not in my back yard! Date: 9 Jul 1995 15:07:04 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 8 Sender: root@newsbf02.news.aol.com Message-ID: <3tp9co$97l@newsbf02.news.aol.com> References: Reply-To: whoff26753@aol.com (WHoff26753) NNTP-Posting-Host: newsbf02.mail.aol.com In fact the growth of amanita has been found in our yard under a large oak tree. It would be difficult to cut down this tree, which is close to one hundred feet high, and there are adjacent trees all over the lot. In addition, it seems that cutting down the tree would still leave an extensive root system and plenty of mycorhizal opportunities. We appreciate your suggestions, in particular the possibility of competing species from Nathan Wilson. We have tried fungicide once but had fruiting Amanita within a week. Any other ideas would be welcomed. From owner-mycology@net.bio.net Sat Jul 08 23:00:00 1995 Path: biosci!STUDENT.MSU.EDU!jiangchu From: jiangchu@STUDENT.MSU.EDU (Chunxiao Jiang) Newsgroups: bionet.mycology Subject: (none) Date: 8 Jul 1995 21:09:02 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <9507090407.AA16001@pilot08.cl.msu.edu> NNTP-Posting-Host: net.bio.net subscribe employment From owner-mycology@net.bio.net Sat Jul 08 23:00:00 1995 Path: biosci!STUDENT.MSU.EDU!jiangchu From: jiangchu@STUDENT.MSU.EDU (Chunxiao Jiang) Newsgroups: bionet.mycology Subject: (none) Date: 8 Jul 1995 20:40:05 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <9507090338.AA94006@pilot05.cl.msu.edu> NNTP-Posting-Host: net.bio.net subscribe employment From owner-mycology@net.bio.net Sat Jul 08 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!news.uoregon.edu!vixen.cso.uiuc.edu!prairienet.org!egrunden From: egrunden@prairienet.org (Eric Grunden) Newsgroups: bionet.mycology Subject: Mycoparasites Date: 9 Jul 1995 03:38:13 GMT Organization: University of Illinois at Urbana Lines: 17 Message-ID: <3tniv5$oe3@vixen.cso.uiuc.edu> Reply-To: egrunden@prairienet.org (Eric Grunden) NNTP-Posting-Host: firefly.prairienet.org Does anyone know where I can find a list of the mycoparasites (fungicolous fungi)? Does anyone else feel that there is a great potential for using them to fight fungal disease in plants and animals? Has it been tried? Who/where/when/results? = eg = -- ******************* The Spirit of Nature, a powerful force, Belongs and returns to its creative source. - Excerpted from The Collective Works of Johnny Pokerface - From owner-mycology@net.bio.net Sun Jul 09 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!lamarck.sura.net!news.uky.edu!chuck From: Chuck Staben Newsgroups: bionet.mycology Subject: Re: DNA from herbarium specimens Date: 10 Jul 1995 12:12:34 GMT Organization: University of Kentucky Lines: 9 Message-ID: <3tr5fi$s6d@service1.uky.edu> References: NNTP-Posting-Host: bs6.mi.uky.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) John Taylor's lab has very wide experience with preparing DNA from many fungi, although perhaps not dried specimen's. You can e-mail him: jtaylor@violet.berkeley.edu. I use a simple procedure like one that John published in which one simply incubates dried mycelium in 50 mM Tris (pH=8), 50 mM EDTA, 2% SDS, 1% 2-mercaptoethanol, 100 microgram/ml proteinase K at 60 degrees overnight. Phenol extract 2X. CHCl3. Ethanol precipitate. Can further purify on CsCl gradients for large preps. This also works for my lab to do minipreps from conidia for PCR. From owner-mycology@net.bio.net Sun Jul 09 23:00:00 1995 Newsgroups: bionet.mycology Path: biosci!rutgers!gatech!news.sprintlink.net!uunet!in1.uu.net!newsflash.concordia.ca!CC.UMontreal.CA!news.uqam.ca!news From: "SŽbastien Chartrand" Subject: Mycologists Montreal/Quebec Content-Type: text/plain; charset=iso-8859-1 Message-ID: Sender: news@news.uqam.ca Nntp-Posting-Host: 132.208.165.29 Content-Transfer-Encoding: 8bit Organization: mcgill Mime-Version: 1.0 Date: Mon, 10 Jul 1995 17:39:32 GMT X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) X-Url: news://news/bionet.mycology Lines: 10 Hi, I'm looking for amateur mycologysts in the area of Montreal or the province to know things about the good places to pick mushrooms. Unfortunetly, my only sucess so far this year is a "polyporus squamosus" I eated, found on the Mount-Royal. (Don't laugh!!) : ) Sébastien Chartrand From owner-mycology@net.bio.net Sun Jul 09 23:00:00 1995 Newsgroups: bionet.mycology Path: biosci!rutgers!gatech!news.sprintlink.net!uunet!in1.uu.net!newsflash.concordia.ca!CC.UMontreal.CA!news.uqam.ca!news From: "SŽbastien Chartrand" Subject: Mycologists Montreal/Quebec Content-Type: text/plain; charset=iso-8859-1 Message-ID: Sender: news@news.uqam.ca Nntp-Posting-Host: 132.208.165.29 Content-Transfer-Encoding: 8bit Organization: mcgill Mime-Version: 1.0 Date: Mon, 10 Jul 1995 17:39:21 GMT X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) X-Url: news://news/bionet.mycology Lines: 10 Hi, I'm looking for amateur mycologysts in the area of Montreal or the province to know things about the good places to pick mushrooms. Unfortunetly, my only sucess so far this year is a "polyporus squamosus" I eated, found on the Mount-Royal. (Don't laugh!!) : ) Sébastien Chartrand From owner-mycology@net.bio.net Sun Jul 09 23:00:00 1995 Path: biosci!bcm!news.msfc.nasa.gov!elroy.jpl.nasa.gov!sdd.hp.com!spool.mu.edu!news.moneng.mei.com!news.ecn.bgu.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!news.sprintlink.net!malgudi.oar.net!corky.mco.edu!NewsWatcher!user From: rodenbaugh@opus.mco.edu (Garry T Cole) Newsgroups: bionet.mycology Subject: Postdoctoral Research Position in Medical Mycology Date: Mon, 10 Jul 1995 14:46:25 -0400 Organization: MCO Lines: 15 Message-ID: NNTP-Posting-Host: 136.247.30.74 A postdoctoral fellowship is available immediately in the Department of Microbiology at the Medical College of Ohio (MCO). The focus of the research project is identification, cloning and expression of T-cell reactive protein genes from coccidioides immitis. This fungus is the causative agent of a human repiratory infection which is endemic to the southwest of the united states. The aim of the project is to develop a vaccine against coccidioidomycosis which will be tested in a murine model. The salary for the fellowship is $25,000 per annum. Please send your C.V. and 3 letters of reference to Dr Garry T. Cole, Professor and Chairman of Microbiology, MCO, 3000 arlington Avenue, Toledo, Ohio, 43199. Or E-mail the address given above. From owner-mycology@net.bio.net Sun Jul 09 23:00:00 1995 Path: biosci!rutgers!gatech!newsxfer.itd.umich.edu!zip.eecs.umich.edu!panix!not-for-mail From: arpad@panix.com (Arpad Jaromy) Newsgroups: bionet.mycology,rec.arts.books.marketplace,rec.collecting.stamps Subject: Book on mushroom stamps Date: 10 Jul 1995 13:54:00 -0400 Organization: PANIX Public Access Internet and Unix, NYC Lines: 4 Message-ID: <3trpfo$2q9@panix.com> NNTP-Posting-Host: panix.com Summary: book on mushroom stamps Keywords: book on mushroom stamps Xref: biosci bionet.mycology:2432 rec.arts.books.marketplace:14436 rec.collecting.stamps:11006 :Looking for the Stanley Gibbons Thematic Catalogue: Collect Fungi on Stamps. Where can I buy it? Do you have any information? Arpad From owner-mycology@net.bio.net Sun Jul 09 23:00:00 1995 Path: biosci!ESSEX.HSC.COLORADO.EDU!claudes From: claudes@ESSEX.HSC.COLORADO.EDU (Claude Selitrennikoff) Newsgroups: bionet.mycology Subject: suggestions for antibody-chromophore Date: 10 Jul 1995 10:30:20 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Groups: We are trying to develop an antigen capture diagnostic test and would like help in designing the "best" and easiest antibody-chromophore detection antibody. We have an IgG and an IgM that we would like to couple to a chromophore that can be easily seen. Suggestions would be greatly appreciated. Thanks Claude Selitrennikoff University of Colorado Health Sci Center Denver, CO From owner-mycology@net.bio.net Sun Jul 09 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!news.sprintlink.net!howland.reston.ans.net!Germany.EU.net!news.dfn.de!uni-muenster.de!TUD2.UNI-MUENSTER.DE!tudzyns From: tudzyns@uni-muenster.de (Prof. Dr. P. Tudzynski) Newsgroups: bionet.mycology Subject: ECFG 3 3rd European Congress of Fungal Genetics in Muenster, Germany Date: Mon, 10 Jul 1995 14:44:33 GMT Organization: Inst. allg. Botanik Lines: 14 Message-ID: NNTP-Posting-Host: tud2.uni-muenster.de X-Newsreader: Trumpet for Windows [Version 1.0 Rev B final beta #4] ECFG 3 3rd European Congress of Fungal Genetics in Muenster, Germany March 27 - 30, 1996 contact adress: Prof. Dr. Paul Tudzynski Westfälische Wilhelms-Universität Institut f. Botanik Schlossgarten 3 48149 Muenster / Germany Fax: 0251 / 83 3823 Email: tudzyns@uni-muenster.de From owner-mycology@net.bio.net Mon Jul 10 23:00:00 1995 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!news.uoregon.edu!psgrain!nntp.teleport.com!usenet From: "Ralph D. Arnold" Newsgroups: bionet.mycology Subject: SERIOUS OUTBREAK OF GLIOCLADIUM... Date: Tue, 11 Jul 95 00:08:04 PST Organization: Teleport - Portland's Public Access (503) 220-1016 Lines: 41 Message-ID: <17115.rarnold@teleport.com> Reply-To: NNTP-Posting-Host: ip-pdx7-32.teleport.com X-Minuet-Version: Minuet1.0_Beta_17A X-POPMail-Charset: English To: poei@telebyte.nl I was asked to post this for someone presently without newsgroup access ----- Forwarded message begins here ----- From: Peter Oei SERIOUS OUTBREAK OF GLIOCLADIUM IN PLEUROTUS CULTIVATION IN THE NETHERLANDS One of the main oyster mushroom substrate producers has serious infection problems for months. Five to seven days after spawning an green mould occurs in all the bags, which was identified as Gliocladium flavum by dr. Geels of the experimenting station in Horst. The massive outbreak lets one to believe the infection occurs in the substrate preparation. Some strange things occured: when he switched from P. ostreatus to P. pulmonarius, things would go much better. But after having grown for some months the pulmonarius, he got the same problems. So he switched back to P. ostreatus, and things went better again. When he inoculated half a batch with ostr. and the other half with pulm, he found in the beginning that all the P. ostreatus would spoil and all the pulm would grow out well. later this pattern reversed. If straw was left unspawned then no infection would occur. The mould seems to parasitize the mycelium of the oyster mushroom, and being capable to adapting itself to another species of Pleurotus. It seems there is a feedback loop (reinfection at the farm), but several experts have failed to point their finger at a contamination source. Before spawning the spawning room is completely sterilised with H2O2, the room has absolute filters, hygienic measures at the farm are OK, so nobody understands where the infection occurs. The straw is brought in batches every two weeks, straw from several places has been used and found to give the same results. Substrate preparation takes place in a giant turning container (18 tons of substrate each time) The system worked fine for 7 years, excluding some problems with spawn containing bacteria. The straw is heated in a dry stage with steam and then cooled with cold water. The water is from a well 35 meters deep. Does anybody know more about Gliocladium flavum or the above mentioned problem? More than 12 growers and their families depend on this substrate producer. Please send comments to: Peter Oei poei@telebyte.nl ------ Forwarded message ends here ------ From owner-mycology@net.bio.net Mon Jul 10 23:00:00 1995 Newsgroups: bionet.mycology Path: biosci!agate!howland.reston.ans.net!swrinde!hookup!nstn.ns.ca!cs.dal.ca!usenet From: Ira Redwood Subject: Digestive enzymes Content-Type: TEXT/PLAIN; charset=US-ASCII Message-ID: Sender: usenet@cs.dal.ca (USENET News) Nntp-Posting-Host: ug.cs.dal.ca Organization: Math, Stats & CS, Dalhousie University, Halifax, NS, Canada Mime-Version: 1.0 Date: Tue, 11 Jul 1995 13:53:01 GMT Lines: 14 Greetings Could anyone tell me if there is a fungal enzyme that resembles those produced by the human pancreas? **************************************************************************** Ira (Red Dog) Redwood | My Heart Is: Advanced Major | Environmental Science | Canadian First, Eh! Dalhousie University | Halifax, Nova Scotia | Albertan Second, Bud! Canada | redwood@ug.cs.dal.ca | Nova Scotian T'ird, B'ye! **************************************************************************** From owner-mycology@net.bio.net Mon Jul 10 23:00:00 1995 Path: biosci!CC.NTU.EDU.TW!r3603207 From: r3603207@CC.NTU.EDU.TW ("C.C.Chen") Newsgroups: bionet.mycology Subject: Enzyme Localization Date: 11 Jul 1995 07:26:59 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I want to localize the enzyme of fungi .( Geotrichum ) How can I do it? Can anyone tell me? I want to use HPLC to separate the melanoidins. What kinds of column can I use it ? From owner-mycology@net.bio.net Mon Jul 10 23:00:00 1995 Path: biosci!CC.NTU.EDU.TW!r3603207 From: r3603207@CC.NTU.EDU.TW ("C.C.Chen") Newsgroups: bionet.mycology Subject: Monospore isolation? Date: 11 Jul 1995 07:10:15 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net ---------- Forwarded message ---------- Does anyone have experience about manipulation of monospore isolation? How to do monospore isolation? Can anyone tell me? I have culture of Geotricum sp.in malt extract agar slants, and after 3 days of incubation at 30 degree celsius, I used 1% triton solution to obtain the spores. The spores was incubated in malt extract agar but the germination rate was very poor. Did I do anything wrong in the procedure? From owner-mycology@net.bio.net Mon Jul 10 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!news.sprintlink.net!uunet!in1.uu.net!news.shout.net!news From: koeltz Newsgroups: bionet.mycology Subject: Koeltz mycology catalog Date: 11 Jul 1995 13:03:26 GMT Organization: koeltz Scientific Books USA Lines: 4 Message-ID: <3ttsqu$6uc@treflan.shout.net> NNTP-Posting-Host: sgtdial02.shout.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) our mycology catalog is now online at http://www.shout.net/~koeltz/ we also have a stock clearing sale on one of our books. From owner-mycology@net.bio.net Mon Jul 10 23:00:00 1995 Path: biosci!WWG3.UOVS.AC.ZA!BRENDA From: BRENDA@WWG3.UOVS.AC.ZA ("BRENDA WINGFIELD - PERSONEEL") Newsgroups: bionet.mycology Subject: DNA from Herbarium specimens Date: 11 Jul 1995 03:56:58 -0700 Organization: Univ. of the Orange Free State Lines: 42 Sender: daemon@net.bio.net Distribution: world Message-ID: <2E45D7B79BB@wwg3.uovs.ac.za> NNTP-Posting-Host: net.bio.net We have done quite a few PCRs from herbarium material. In my experience some of the best sucesses I have had is directly from the old material itself, that is do not extract the DNA just add the "mycelium". We have reported on this a couple of times and it really works well. My suggestion over and above not extracting is to use a smaller region. That is rather than ITS1 to ITS4 (which I presume is what you are using) use ITS1 to ITS2 and then ITS3 to ITS4. Another problem we have encountered which we have not been able to resolve is that ITS1 and ITS4 simply do not work with some ascomycetes that we are interested in. You do not say what group of organisms you are working with. Good luck Brenda Wingfield . > To: mycology@net.bio.net > From: lorelei@TELEPORT.COM (Lorelei Norvell) > Subject: DNA from herbarium specimens > Date: 9 Jul 1995 15:25:44 -0700 > > I have been successful in extracting and amplifying nDNA (ITS1 + 5.8srRNA > + ITS2) from over 100 different dried herbarium specimens. However, I > have found that the older the DNA, the greater problems I have > encountered. Unfortunately it is imperative I extract DNA from type > specimens collected from 1910 to 1970. While I have had some success > with the 1970 and 1956 collections, I am encountering problems with > degraded DNA. > I would welcome advice with respect to > [1] Whether drying the specimens again before extraction would increase > success or further degrade the DNA (drying with no heat). > [2] Another procedure besides the hot CTAB procedure I have used > [3] Any other preparation advice. > > Thanks. > Lorelei Norvell > University of Washington > Department of Botany > > From owner-mycology@net.bio.net Mon Jul 10 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!gatech!news-feed-1.peachnet.edu!news.duke.edu!usenet From: elcooper@chem.duke.edu (Evan L. Cooper) Newsgroups: bionet.mycology Subject: Yes! Chantarelles NOT Jack O'Lantern Date: 11 Jul 1995 10:10:51 GMT Organization: Duke University / Chem. Dept. Lines: 20 Message-ID: <3ttinb$nd5@news.duke.edu> NNTP-Posting-Host: async49.async.duke.edu Mime-Version: 1.0 Content-Type: Text/Plain; charset=ISO-8859-1 X-Newsreader: WinVN 0.99.5 Greetings! Thanks to everyone for their help with my question regarding identification of my mushrooms. After reading the posted and emailed replies I became pretty sure that they were Chantarelles. I went over to the Duke University Biology department looking for Prof. Rytas Vilgalys. He is on sabbatical until August. Members of his research group were very helpful, though. Particularly one experienced field collector who positively identified a couple of specimens I brought in as Chantarelle. They have the blunt forked folds instead of gills as everyone mentioned. They are growing out of the ground and not attached to wood as some stated. They did have a faint apricot-like odor. And, they are delicious. Nice peppery taste. Yum! Thanks again! Evan L. Cooper (elcooper@chem.duke.edu) From owner-mycology@net.bio.net Mon Jul 10 23:00:00 1995 Path: biosci!rutgers!gatech!news.sprintlink.net!dish.news.pipex.net!pipex!uknet!bhamcs!news.ox.ac.uk!oxpath!casselton From: casselton@molbiol.ox.ac.uk Newsgroups: bionet.mycology Subject: homeodomain proteins-postdoc Date: 11 Jul 95 21:04:30 GMT Organization: Oxford University Molecular Biology Data Centre Lines: 19 Message-ID: <1995Jul11.210430@ania.path.ox.ac.uk> NNTP-Posting-Host: ania.path.ox.ac.uk POSTDOCTORAL RESEARCH ASSISTANT - FUNGAL MOLECULAR GENETICS Applications are invited for a postdoctoral position to study the interactions of homeodomain proteins that regulate sexual development and determine self-nonself recognition in the mushroom Coprinus cinereus. The post is funded by BBSRC for two years and is available immediately. Salary will be on the RS 1A scale. The project is jointly supervised by Dr Lorna Casselton in the Department of Plant Sciences and Dr Jane Mellor in the Department of Biochemistry. Applications, including a detailed curriculum vitae and names of two referees, should be sent to the Administrator, Department of Plant Sciences, South Parks Road, Oxford OX1 3RB. Closing date 18 August 1995. Enquiries: Lorna Casselton: Tel 01865 275 109; FAX 01865 275 074; e-mail Casselton@molbiol.ox.ac.uk From owner-mycology@net.bio.net Mon Jul 10 23:00:00 1995 Path: biosci!rutgers!gatech!news.uoregon.edu!gaia.ucs.orst.edu!news.PEAK.ORG!PEAK.ORG!ddelace From: Dave Delacey Newsgroups: bionet.mycology Subject: test Date: Tue, 11 Jul 1995 14:00:33 -0700 Organization: CS Outreach Services--PEAK, Corvallis, Oregon, USA Lines: 1 Message-ID: NNTP-Posting-Host: kira Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII hi there From owner-mycology@net.bio.net Mon Jul 10 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!newsxfer.itd.umich.edu!caen!malgudi.oar.net!corky.mco.edu!NewsWatcher!user From: rodenbaugh@opus.mco.edu (Garry T Cole) Newsgroups: bionet.mycology Subject: Research Technician Required Date: Tue, 11 Jul 1995 17:21:00 -0400 Organization: MCO Lines: 16 Message-ID: NNTP-Posting-Host: 136.247.30.74 Animal Research position at the Medical College of Ohio (MCO) A technician/research position is available immediately at MCO involving murine studies of both antifungal drug efficacy and T-cell immunity. The latter is part of a research project focused on fungal vaccine development. Minimal requirement is a Bachelor of Science degree with at least 3 years of postgraduate experience in animal research. Salary is commensurate with experience. Excellent fringe benefits. Send C.V. and 3 letters of reference to Dr Garry T. Cole, Professor and Chairman of Microbiology, MCO, 3000 Arlington Avenue, Toledo, OH, 43199. Or E-mail address given above. From owner-mycology@net.bio.net Tue Jul 11 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!news.moneng.mei.com!howland.reston.ans.net!dish.news.pipex.net!pipex!warwick!news.shef.ac.uk!silver!mb935645 From: mb935645@silver.shef.ac.uk (D L Clarke) Newsgroups: bionet.mycology Subject: Transcription start site determination. Date: 12 Jul 1995 12:38:26 GMT Organization: University of Sheffield, UK Lines: 12 Message-ID: <3u0fo2$82c@hippo.shef.ac.uk> NNTP-Posting-Host: silver.shef.ac.uk X-Newsreader: TIN [version 1.2 PL2] Dear all, at present I am characterising a gene from Aspergillus nidulans. for publication the transcription start site must be determined. Does anybody know of a good method or kit from which I can map the start site. Thanks Dave Clarke E-mail D.L.Clarke@Sheffield.ac.uk From owner-mycology@net.bio.net Tue Jul 11 23:00:00 1995 Path: biosci!rutgers!gatech!news.sprintlink.net!dish.news.pipex.net!pipex!oleane!jussieu.fr!univ-lyon1.fr!news.imag.fr!ciril.fr!paxillus.scbiol.u-nancy.fr!user From: richard@scbiol.u-nancy.fr (RICHARD Thierry) Newsgroups: bionet.mycology Subject: Postdoctoral position research Date: Wed, 12 Jul 1995 19:15:33 +0200 Organization: Lab bio Forest - Universite Henri Poincare - Nancy (France) Lines: 20 Message-ID: NNTP-Posting-Host: paxillus.scbiol.u-nancy.fr Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit Postdoctoral position research Working currently as a PhD student on the lectins of fungi, I intend to defend my thesis in november 1995. Over the last for years, I have purified, characterized the lectin of Rigidoporus lignosus, a phytopathogenic fungus of tropical plantations which differentiates rhizomorphs. We have gained evidence that this lectin is likely involved in the process of rhizomorph differentiation and in the process of host-parasitism by the immunolocalization of the lectin on the fungus cells wall and its affinity sites on the fungus cells wall and on the cells wall of Hevea roots. Would it be possible for a laboratory to propose me a postdoctoral position starting from november 1995. I can send my Curriculum vitae with some details of my background if someone is interested. I thank you very much in anticipation and remain yours truly. RICHARD Thierry (richard@scbiol.u-nancy.fr; Tel: 83 91 22 39) From owner-mycology@net.bio.net Tue Jul 11 23:00:00 1995 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!dish.news.pipex.net!pipex!oleane!jussieu.fr!univ-lyon1.fr!news.imag.fr!ciril.fr!paxillus.scbiol.u-nancy.fr!user From: Pascale.Jacquot@scinfo.u-nancy.fr (Pascale Jacquot) Newsgroups: bionet.mycology Subject: Postdoctoral position Research Date: Wed, 12 Jul 1995 19:02:29 +0200 Organization: Scinfo - ESIAL - Universite Henri Poincare - Nancy (France) Lines: 18 Message-ID: NNTP-Posting-Host: paxillus.scbiol.u-nancy.fr Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit Working currently as a PhD student on the lectins of fungi, I intend to defend my thesis in november 1995. Over the last for years, I have purified, characterized the lectin of Rigidoporus lignosus, a phytopathogenic fungus of tropical plantations which differentiates rhizomorphs. We have gained evidence that this lectin is likely involved in the process of rhizomorph differentiation and in the process of host-parasitism by the immunolocalization of the lectin on the fungus cells wall and its affinity sites on the fungus cells wall and on the cells wall of Hevea roots. Would it be possible for a laboratory to propose me a postdoctoral position starting from november 1995. I can send my Curriculum vitae with some details of my background if someone is interested. I thank you very much in anticipation and remain yours truly. RICHARD Thierry (richard@scbiol.u-nancy.fr; Tel: 83 91 22 39) From owner-mycology@net.bio.net Tue Jul 11 23:00:00 1995 Path: biosci!rutgers!gatech!howland.reston.ans.net!agate!nntp-ucb.barrnet.net!well!peter From: peter@well.sf.ca.us (Peter Febbroriello) Newsgroups: bionet.mycology Subject: Research technician available Date: 12 Jul 1995 14:08:35 GMT Organization: The Whole Earth 'Lectronic Link, Sausalito, CA Lines: 5 Message-ID: <3u0l13$vt@nkosi.well.com> NNTP-Posting-Host: well.com After noting several requests for technicians in differnt forums, I thought I might offer my services at a distance. My lab has a little extra capacity to run some experiments if anyone needs a little extra help with a project.--pf From owner-mycology@net.bio.net Tue Jul 11 23:00:00 1995 Path: biosci!ESSEX.HSC.COLORADO.EDU!claudes From: claudes@ESSEX.HSC.COLORADO.EDU (Claude Selitrennikoff) Newsgroups: bionet.mycology Subject: anticapsin Date: 12 Jul 1995 14:24:37 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Group: A number of years ago, a compound of fungal amidotransferase (the first pathway specific enzyme of the Leloir Pathway) called anticapsin was described as an inhibitor. Does anyone know where I can obtain a sample--it is not in a number of obvious places, e.g., Sigma, Aldrich, etc.? Thanks for your help Claude Selitrennikoff University of Colorado Health Sci Center From owner-mycology@net.bio.net Tue Jul 11 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: tomcoleman@aol.com (TomColeman) Newsgroups: bionet.mycology Subject: Re: Unknown fungus Date: 12 Jul 1995 19:26:37 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 6 Sender: root@newsbf02.news.aol.com Message-ID: <3u1lnd$hrf@newsbf02.news.aol.com> References: <3t4iu6$j8u@newsbf02.news.aol.com> Reply-To: tomcoleman@aol.com (TomColeman) NNTP-Posting-Host: newsbf02.mail.aol.com What you have been given is a fungus commonly called Kombucha. This is a questionable endeavor. I suggest you read an article in a magazine called "Mushroom- The Journal of Wild Mushrooming" entitled "My Adventures with the Blob" by Paul Stamets. It is in Issue 46, Vol.13, No. 1 Winter 94-95. Address is Mushroom, 861 Harold St. Moscow, ID. I would refrain from drinking this tea until you read this article. TJC From owner-mycology@net.bio.net Wed Jul 12 23:00:00 1995 Newsgroups: bionet.mycology Path: biosci!bloom-beacon.mit.edu!gatech!howland.reston.ans.net!swrinde!dish.news.pipex.net!pipex!demon!btnet!gold.compulink.co.uk!cix.compulink.co.uk!usenet From: ecoli@cix.compulink.co.uk ("K N and P J Harris") Subject: DNA from herbarium specimens. Message-ID: Organization: Compulink Information eXchange X-Newsreader: WigWam Date: Thu, 13 Jul 1995 08:24:31 GMT Lines: 14 "Old DNA is of a wider interest than you might think. The soil microbiology world is awash with interest in extracting DNA from soil in the hopes of using it as a way of coping with the hitherto intractable problem of diversity in the soil population. Problem is that we know that some of the humus organic matter is hundreds, if not thousands of years old. Some of that old organic matter could be DNA, messed up and with all sorts of crud attached to it but still DNA. Now, the problem is that,if you extract and purify DNA, how on earth (no pun intended) do you know whether it is relevant to the current population or whether it is a good historical record of what was the population before Columbus sailed ? Peter Harris, Environmental Microbiologist, University of Reading, UK AKA From owner-mycology@net.bio.net Wed Jul 12 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!spool.mu.edu!usenet.eel.ufl.edu!interlog.com!io.org!prevop.net3.io.org!bpretto From: bpretto@io.org (Bruno Pretto & Paula Vopni) Newsgroups: bionet.mycology Subject: Desert Truffles Date: Thu, 13 Jul 1995 08:05:50 Organization: Mushrooms/Envirosearch Lines: 12 Message-ID: NNTP-Posting-Host: prevop.net3.io.org X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] My next door neighbour was in Syria on an archeologigal expedition. At the market he was able to locate some desert truffles for me. He returned with one specimen weighing approx. 500 grams, for which he paid $ 10.00. It has a smooth outside skin unlike the french truffles. I plan to take some photographs of it before consuming it and I will post them on the net. Can anyone tell me the latin name for this truffle. Bruno Pretto, Fun Guy Farm (Toronto,Canada). Home page http://www.io.org/~bpretto NAMA Trustee for Toronto Mycological Society. Secretary Oak Forest Mushroom Association (outdoor shiitake growers). From owner-mycology@net.bio.net Wed Jul 12 23:00:00 1995 Path: biosci!rutgers!gatech!usenet.eel.ufl.edu!usenet.cis.ufl.edu!caen!news.tc.cornell.edu!newsserver.sdsc.edu!nic-nac.CSU.net!charnel.ecst.csuchico.edu!csusac!csus.edu!news.ucdavis.edu!jerome.ucdavis.edu!user From: jfrigot@ucdavis.edu (Jerome Rigot) Newsgroups: bionet.mycology Subject: Biomarker to monitor presence and activity in environment Date: Thu, 13 Jul 1995 14:12:53 -0800 Organization: UCDavis Lines: 21 Message-ID: NNTP-Posting-Host: jerome.ucdavis.edu Keywords: Filamentous fungi, biomarker, activity, release, environment, monitoring Hello, I am looking for references related to the insertion of a biomarker into a filamentous fungus to monitor its presence and activity in the environment after release. This could be applied to the monitoring biocontrol organisms in soil or to the monitoring of degradative organisms released in the environment for bioremediation purpose. Lux gene is used for bacterial systems, and luc gene (luciferase) may potentially be used for fungi or yeasts. Are there any other approaches? I found only one reference about luc gene inserted in yeast. I would appreciate suggestions and specific references. Respond directly to my email address. Thanks. Jerome Rigot -- Jerome Rigot Dept. Environmental Toxicology University of California Davis, CA 95616 (916)752-5896 e-mail: jfrigot@ucdavis.edu From owner-mycology@net.bio.net Wed Jul 12 23:00:00 1995 Path: biosci!ESSEX.HSC.COLORADO.EDU!claudes From: claudes@ESSEX.HSC.COLORADO.EDU (Claude Selitrennikoff) Newsgroups: bionet.mycology Subject: APT paper Date: 13 Jul 1995 14:36:58 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Group: Does anyone know a commercial source for aminophenylthioether paper (APT cellulose) and/or diazophenylthioether paper? Thanks for your help Claude Selitrennikoff From owner-mycology@net.bio.net Wed Jul 12 23:00:00 1995 Path: biosci!forestry.utoronto.ca!dobranic From: dobranic@forestry.utoronto.ca (Jason Dobranic) Newsgroups: bionet.mycology Subject: Mushroom equipment Date: 13 Jul 1995 12:38:17 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: <9507131944.AA19529@forestry.utoronto.ca> NNTP-Posting-Host: net.bio.net Hello, Can anyone recommend the best company to buy mushrooms growing equipment and supplies from? I am in the Toronto, Canada so is there any  in the area? Thanks! Jason From owner-mycology@net.bio.net Wed Jul 12 23:00:00 1995 Path: biosci!SPLAVA.CC.PLATTSBURGH.EDU!SEIDELBL From: SEIDELBL@SPLAVA.CC.PLATTSBURGH.EDU (Bonnie Seidel-Rogol) Newsgroups: bionet.mycology Subject: antibodies to spores Date: 13 Jul 1995 11:03:24 -0700 Organization: SUNY at Plattsburgh, New York, USA Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HSTO7RSC9U8X10R5@splava.cc.plattsburgh.edu> NNTP-Posting-Host: net.bio.net Date sent: 13-JUL-1995 13:56:25 I am interested in using fluorescent antibodies against fungal and bacterial spores for detection in environmental and food samples. I'm looking for a commercial source of such antibodies, or a company that will synthesize the antibodies. I'm from a small undergraduate institution and do not have the facilities (or the expertise) for making my own antibodies. Any information/suggestions would be greatly appreciated. Bonnie L. Seidel-Rogol Dept. Biol. Sci. SUNY-Plattsburgh Plattsburgh, N.Y. 12901 EMail: Seidelbl@splava.cc.plattsburgh.edu" From owner-mycology@net.bio.net Wed Jul 12 23:00:00 1995 Path: biosci!SEMOVM.SEMO.EDU!C749SCB From: C749SCB@SEMOVM.SEMO.EDU (Walt Lilly) Newsgroups: bionet.mycology Subject: Stephen Horton's Email Address Date: 13 Jul 1995 08:59:21 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <13JUL95.11805612.0037.MUSIC@SEMOVM> NNTP-Posting-Host: net.bio.net Hi..... Does anyone have Steve Horton's Email address? Are you out there, Steve? Thanks, Walt Lilly From owner-mycology@net.bio.net Thu Jul 13 23:00:00 1995 Path: biosci!TELEPORT.COM!lorelei From: lorelei@TELEPORT.COM (Lorelei Norvell) Newsgroups: bionet.mycology Subject: Re: DNA from herbarium specimens. Date: 13 Jul 1995 18:33:48 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net On Thu, 13 Jul 1995, K N and P J Harris wrote: > "Old DNA is of a wider interest than you might think. The soil > microbiology world is awash with interest in extracting DNA from soil in > the hopes of using it as a way of coping with the hitherto intractable > problem of diversity in the soil population. Problem is that we know > that some of the humus organic matter is hundreds, if not thousands of > years old. Some of that old organic matter could be DNA, messed up and > with all sorts of crud attached to it but still DNA. Now, the problem is > that,if you extract and purify DNA, how on earth (no pun intended) do > you know whether it is relevant to the current population or whether it > is a good historical record of what was the population before Columbus > sailed ? Faith. Desire. Subterfuge......? And comparisons to a reference DNA library. Which we don't have yet. Which brings us back to faith desire subterfuge Lorelei From owner-mycology@net.bio.net Thu Jul 13 23:00:00 1995 Path: biosci!rutgers!gatech!swrinde!cs.utexas.edu!usc!nic-nac.CSU.net!usenet From: spencal@nextlab7.calstatela.edu (Steve Pencall) Newsgroups: bionet.mycology Subject: LAMS Spore Print Archives Now Online Date: 14 Jul 1995 01:02:05 GMT Organization: Information Resources and Technology Lines: 30 Distribution: world Message-ID: <3u4fmd$cia@nic-nac.CSU.net> NNTP-Posting-Host: nextlab7.calstatela.edu The Electronic Edition of The Spore Print, Journal of the Los Angeles Mycological Society, is now archived on a gopher site at the Institute for Global Communications. Issues available are Number 189 (October 1993) through Number 207 (June 1995) It's URL is: Via gopher gopher.igc.apc.org/00/pubs/sporeprint/ (Uses default port 70) For World Wide Web users there is a link to it off the IGC Mushroom Homepage. which lives at: http://www.igc.apc.org/igc/www.myco.html If you are using a Gopher browser: Find the Institute for Global Communications gopher site (it is in California) then: EcoNet--Environment--> EcoNet's General Environmental Resources--> EcoNet's Mycology and Mushrooms Gopher--> The Spore Print, Journal of the Los Angeles Mycological Society A very Special Thanks to Michael Stein of the Institute for Global Communications for all his hard work and for making a home for The Spore Print at the IGC! Steven Pencall Editor, The Spore Print Journal of The Los Angeles Mycological Society From owner-mycology@net.bio.net Thu Jul 13 23:00:00 1995 Path: biosci!daresbury!trane.uninett.no!Norway.EU.net!EU.net!howland.reston.ans.net!news.sprintlink.net!news.clark.net!rahul.net!a2i!coconut!aloha.com!not-for-mail From: daka@aloha.com (_doc_) Newsgroups: bionet.mycology Subject: Re: Mushroom equipment Date: 14 Jul 1995 09:32:15 -1000 Organization: A poorly-installed InterNetNews site Lines: 18 Message-ID: <3u6gnv$7sp@aloha.com> References: <9507131944.AA19529@forestry.utoronto.ca> NNTP-Posting-Host: mango.aloha.com In article <9507131944.AA19529@forestry.utoronto.ca>, Jason Dobranic wrote: >Hello, > Can anyone recommend the best company to buy mushrooms growing >equipment and supplies from? I am in the Toronto, Canada so is there any  >in the area? I dont know any in your area, however I would higghly reccomend: Fungi Perfecti P.O. Box 7634 Olympia, WA 98507 USA (206) 426-9292 This company was founded/is run by Paul Staments and his family. The catalog is filled with just about everything you need. Mike From owner-mycology@net.bio.net Thu Jul 13 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!lamarck.sura.net!news.uky.edu!chuck From: Chuck Staben Newsgroups: bionet.mycology Subject: Re: anticapsin Date: 14 Jul 1995 12:13:25 GMT Organization: University of Kentucky Lines: 7 Message-ID: <3u5n15$smf@service1.uky.edu> References: NNTP-Posting-Host: bs6.mi.uky.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) A few years ago Molecular Design offered a software product that searched a database of all chemical suppliers for specific compounds. It could also do structural searches. I believe that they offered this as a contract service as well. You might contact them. I think they now have a WWW site, http://www.ig.com/bioinfo.html From owner-mycology@net.bio.net Thu Jul 13 23:00:00 1995 Path: biosci!daresbury!trane.uninett.no!Norway.EU.net!EU.net!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: unicornbag@aol.com (UNICORNbag) Newsgroups: bionet.mycology Subject: Re: Mushroom equipment Date: 14 Jul 1995 18:59:59 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 1 Sender: root@newsbf02.news.aol.com Message-ID: <3u6stf$1b4@newsbf02.news.aol.com> References: <9507131944.AA19529@forestry.utoronto.ca> Reply-To: unicornbag@aol.com (UNICORNbag) NNTP-Posting-Host: newsbf02.mail.aol.com Western Biologicals Ltd.-Aldergrove, BC- 604 856 3339 Bill Chelmers From owner-mycology@net.bio.net Fri Jul 14 23:00:00 1995 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!agate!nntp-ucb.barrnet.net!well!peter From: peter@well.sf.ca.us (Peter Febbroriello) Newsgroups: bionet.mycology Subject: Kombucha Date: 15 Jul 1995 15:10:28 GMT Organization: The Whole Earth 'Lectronic Link, Sausalito, CA Lines: 4 Message-ID: <3u8lp4$g5h@nkosi.well.com> NNTP-Posting-Host: well.com Kombucha fungus produces heparin and heparin precursors which may cause bleeding problems in some folks, may interfere with heart drugs, or intensify anti clotting protocols...--pf From owner-mycology@net.bio.net Fri Jul 14 23:00:00 1995 Newsgroups: bionet.mycology Path: biosci!rutgers!uwm.edu!cs.utexas.edu!news.tamu.edu!news.utdallas.edu!corpgate!bcarh189.bnr.ca!nott!cunews!freenet.carleton.ca!FreeNet.Carleton.CA!bs459 From: bs459@FreeNet.Carleton.CA (Erica Tan) Subject: Manchurian Mushrooms Message-ID: Sender: bs459@freenet3.carleton.ca (Erica Tan) Organization: The National Capital FreeNet, Ottawa, Ontario, Canada Date: Fri, 14 Jul 1995 14:01:38 GMT Lines: 11 Does anybody know of the longterm effects of Manchurian Mushrooms? My mother used to drink the "mushroom tea", but she gave up on it after a month (too much trouble to keep the darn thing alive). I was wondering if any studies (published) have been conducted on these mushrooms. There seems to be a lot of interest in these mushrooms, but there isnt' much information on them. Thanks in advance. Erica. From owner-mycology@net.bio.net Sat Jul 15 23:00:00 1995 Path: biosci!agate!news.mindlink.net!vanbc.wimsey.com!io.org!prevop.net3.io.org!bpretto From: bpretto@io.org (Bruno Pretto & Paula Vopni) Newsgroups: bionet.mycology Subject: Re: Desert Truffles Date: Sun, 16 Jul 1995 19:57:06 Organization: Mushrooms/Envirosearch Lines: 22 Message-ID: References: NNTP-Posting-Host: prevop.net3.io.org X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] >My next door neighbour was in Syria on an archeologigal expedition. At the >market he was able to locate some desert truffles for me. He returned with one >specimen weighing approx. 500 grams, for which he paid $ 10.00. It has a >smooth outside skin unlike the french truffles. I plan to take some >photographs of it before consuming it and I will post them on the net. >Can anyone tell me the latin name for this truffle. July 16 Have finally photographed and tasted this intriguing fungus. The exterior is dark brown, smooth textured. The interior is beige with white interlacing lines rather like the Italian white truffle. The flavour is very mild but rather pleasant, texture is somewhat crunchy. There is not much aroma to compared to the French black truffle. But here is a good question for all you mycologists. Is this a mychorrizal species, and if it is what is it forming a symbiotic relationship with if there are no trees in the desert. Bruno Pretto, Fun Guy Farm (Toronto,Canada). Home page http://www.io.org/~bpretto NAMA Trustee for Toronto Mycological Society. Secretary Oak Forest Mushroom Association (outdoor shiitake growers). From owner-mycology@net.bio.net Sat Jul 15 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!news.moneng.mei.com!uwm.edu!msunews!harbinger.cc.monash.edu.au!news.uwa.edu.au!r10.dialup.uwa.edu.au!user From: rwills@sunny.gis.uwa.edu.au (rwills@sunny.gis.uwa.edu.au) Newsgroups: bionet.mycology Subject: Anouncement: Senior position, Phytophthora root diseases Date: Sun, 16 Jul 1995 12:50:00 +0800 Organization: Geography Department, The University of Western Australia Lines: 42 Message-ID: NNTP-Posting-Host: r10.dialup.uwa.edu.au Principal Research Scientist/Senior Principal Research Scientist Western Australian Department of Conservation and Land Management This new position has been established to co-ordinate the research on the Phytophthora root diseases. Tenure: Appointment for 5 years, possibility of extension. Western Australian Governement Level 7 or Level 8. Pos. No. CLM3000852 Salary: $Aus 53750 - 57599 (Level 7) $Aus 60856 - 66082 (Level 8) (An alternative Workplace Agreement with a higher salary range may be negotiated) Qualifications/experience: National recognition for excellence of research into the control of fungal pathogens of plants. A degree in biological science or an approved equivalent. Proven skills in effective management of research teams. Proven skills in the allocation and control of research budgets. Experience in the control and management of research budgets. Experience in the control and management of plant diseases in natural plant communities (desirable). A higher degree in a subject relevant to the duties (desirable). Duties: Undertakes research and provides leadership for a team of scientists researching management of plant diseases in natural communities. Co-ordinates the interaction of the team and the transfer of results to management prescriptions. Conditions: Subject to a Workplace Agreement. General: Class 'A' drivers licence essential. Location: Como, Perth, Western Australia. Further information: Dr Jim Armstrong phone (61 - 9) 442 0307 Job description and application forms: phone (61 - 9) 334 0222 Closing Date: 5.00 pm, 27 July 1995 From owner-mycology@net.bio.net Sun Jul 16 23:00:00 1995 Newsgroups: bionet.mycology Path: biosci!rutgers!gatech!howland.reston.ans.net!Germany.EU.net!EU.net!news.sprintlink.net!uunet!in1.uu.net!shore!news3.near.net!emba-news.uvm.edu!news From: rullrich@moose.uvm.edu (Robert C. Ullrich) Subject: Postdoctoral Position - Molecular Genetics of Fungi Message-ID: <1995Jul17.183853.4111@emba.uvm.edu> Sender: news@emba.uvm.edu Organization: Your Organization X-Newsreader: WinVN 0.93.11 Date: Mon, 17 Jul 1995 18:38:53 GMT Lines: 32 Newsgroups: bionet.jobs Subject: NIH-funded Postdoc Date: July 14, 1995 Organization: Departments of Microbiology and Molecular Genetics, and Botany and Agricultural Biochemistry, University of Vermont POSTDOCTORAL RESEARCH FELLOW - FUNGAL MOLECULAR GENETICS A Postdoctoral Fellowship funded from an NIH research grant is available immediately to study regulation of sexual development by homeodomain proteins in the model basidiomycete fungus, Schizophyllum commune. See Proc. Natl. Acad. Sci. USA. 89:7169-7173 and 7174-7178, Molec. Gen. Genet. 244:318-324, and Biochem. Biophys. Res. Commun. 211:1071-1076 for additional background. Experience in all standard techniques of recombinant DNA analysis, protein expression, protein-protein interactions and protein-DNA interactions is essential. Experience with fungi is desirable, but not essential. This project is jointly administered by Drs. Charles P. Novotny, Dept. of Microbiology and Molecular Genetics and Robert C. Ullrich, Dept. of Botany and Agricultural Biochemistry. To apply send curriculum vitae, statement of research interests and three letters of reference to: Dr. Robert C. Ullrich, Department of Botany, University of Vermont, Burlington, VT 05405 0086, phone (802) 656 0432, FAX (802)-656-0440. The University of Vermont is an AAEO employer. From owner-mycology@net.bio.net Sun Jul 16 23:00:00 1995 Path: biosci!rutgers!gatech!news.uoregon.edu!news.u.washington.edu!homer19.u.washington.edu!todell From: Thomas O'Dell Newsgroups: bionet.mycology Subject: Re: Desert Truffles Date: Mon, 17 Jul 1995 10:37:50 -0700 Organization: University of Washington Lines: 8 Message-ID: References: NNTP-Posting-Host: homer19.u.washington.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII NNTP-Posting-User: todell In-Reply-To: Desert truffles, Terfezia and allies, are mycorrhizal with Helianthemum spp (Cistaceae) and probably other plants. There is some literature on this, but I dont have references handy. Not al ectomycorrhizal hosts are trees, or even woody plants, e.g. Kobresia myrosoides (Cyperaceae) is ectomycorrhizal and a sedge relative. todell@u.washington.edu From owner-mycology@net.bio.net Sun Jul 16 23:00:00 1995 Path: biosci!agate!news.duke.edu!solaris.cc.vt.edu!news.alpha.net!uwm.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!lamarck.sura.net!news.uky.edu!chuck From: Chuck Staben Newsgroups: bionet.mycology Subject: Re: Searching FGSC on-line data Date: 17 Jul 1995 15:37:59 GMT Organization: University of Kentucky Lines: 5 Message-ID: <3ue04n$pjf@service1.uky.edu> References: <01HSYZK7LEYQ8X3WJM@KUHUB.CC.UKANS.EDU> NNTP-Posting-Host: bs6.mi.uky.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) I would like to thank Craig and everyone at the Stock Center for the effort in doing this. I think we are all coming to appreciate that electronic data are important, but that careful consideration of how these data will be used is critical to enhancing the value of the data to the user. Thanks again. From owner-mycology@net.bio.net Sun Jul 16 23:00:00 1995 Path: biosci!KUHUB.CC.UKANS.EDU!fgsc From: fgsc@KUHUB.CC.UKANS.EDU (Fungal Genetics Stock Center) Newsgroups: bionet.mycology Subject: Searching FGSC on-line data Date: 17 Jul 1995 07:18:00 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HSYZK7LEYQ8X3WJM@KUHUB.CC.UKANS.EDU> NNTP-Posting-Host: net.bio.net It took a long time to accomplish the task, but listings of three large groups of stocks at FGSC can now be searched via the Stock Center web site. Neurospora crassa mutants, Neurospora wild types, and Aspergillus nidulans strains can be searched. The routine allows for combinations of markers. Examples might be adG and yA in Aspergillus, pab and inl in Neurospora. Partial names are legitimate; searching for pab in either list of mutants would yield all mutants requiring p-aminobenzoate. Specifying pabaA in A. nidulans or pab-1 in N. crassa would return only strains with those specific mutations. Searching is case sensitive. We think this will be a useful addition to our web page, and invite people to use these functions and comment on what does and does not work. The stock center web page can be found at: http://www.kumc.edu/research/fgsc/main.html Craig Wilson From owner-mycology@net.bio.net Sun Jul 16 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!usc!cs.utexas.edu!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!uunet!in1.uu.net!news.uiowa.edu!news From: michael-clark@uiowa.edu (Michael Clark) Newsgroups: bionet.mycology Subject: Morel mycelium Date: 17 Jul 1995 01:31:50 GMT Organization: University of Iowa, Iowa City, IA, USA Lines: 13 Sender: -Not-Authenticated-[6705] Distribution: world Message-ID: <3ucei6$e4d@nexus.uiowa.edu> NNTP-Posting-Host: 128.255.93.18 X-Posted-From: InterNews 1.0.1@128.255.93.18 Xdisclaimer: No attempt was made to authenticate the sender's name. Hello, Sorry if this seems like a stupid question, but I've recently taken a tissue culture from a black morel, and the mycelium looks very different from other mushroom mycelium I've seen (C. comatus, A. brun., S. rugoso-annulata). It is much less dense, whitish brown, and appears to grow into the agar as well as on top of it. My media is standard malt-extract agar supplimented with yeast extract with ampicillin (200mg/liter of agar) added to suppress bacterial contamination. Is this appearance normal for morel mycelium, and are there any recommendations on agar media? Thanks. Michael Clark michael-clark@uiowa.edu From owner-mycology@net.bio.net Sun Jul 16 23:00:00 1995 Path: biosci!MIZAR.USC.EDU!dreynold From: dreynold@MIZAR.USC.EDU (Don R. Reynolds) Newsgroups: bionet.mycology Subject: Kombucha Date: 17 Jul 1995 14:50:43 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: <199507172106.OAA15022@mizar.usc.edu> NNTP-Posting-Host: net.bio.net Kombucha fungus produces heparin and heparin precursors which may cause bleeding problems in some folks, may interfere with heart drugs, or intensify anti clotting protocols...--pf Don R. Reynolds Natural History Museum 900 Exposition Boulevard Los Angeles, California 90007 213 744 3232 http://www.usc.edu/lacmnh dreynold@bcf.usc.edu From owner-mycology@net.bio.net Sun Jul 16 23:00:00 1995 Path: biosci!hawaii.edu!roxanne From: roxanne@hawaii.edu (Roxanne Yamashita) Newsgroups: bionet.mycology Subject: Postdoctoral Research Fellow Date: 17 Jul 1995 12:52:36 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 69 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net July 17, 1995 Dear Drs. Ullrich and Novotny, Hello! I am responding to the posting for a Postdoctoral position you advertised. I am currently working on my Ph.D. at the University of Hawaii and plan to finish in August of this year. I completed my Masters degree in December of 1993 studying the expression of foreign heterologous proteins in Neurospora crassa. Some of the things I looked at involved codon bias, promoters, and signal sequences. I am very familiar with using various computer programs which look at protein structure based on alignment at both the DNA and protein level and the use of neural networks. My Ph.D. work involves the use and recognition of signal sequences in Neurospora crassa. I am currently trying to improve upon the work of von Heinje's weighted matrix approach in the identification of signal peptides. This approach works well for many proteins of many organisms, but Neurospora seems to be an exception. I am also trying to increase the data we have of signal sequences in Neurospora, to date only 6 of approximately 20 genes for secreted proteins have been identified. Using degenerate oligomers based on N-terminal sequences I am probing Lambda Zap libraries, looking for homology via GCG, and trying to apply this data to a program which will identify signal sequences in Neurospora. I have also isolated and sequenced the cDNA clone of an aldolase gene in N. crassa and I am currently isolating the genomic clone. I am also mapping the gene by RFLP analysis. I have a strong molecular biology background. I have experience in RNA extraction, PAGE, PCR, Westerns, Northerns, Southerns, plasmid construction (Masters work) and mapping (Ph.D. work). Although I have enjoyed working with Neurospora for the past 4 years, I would welcome the opportunity to try something new. I have enclosed a list of reference contacts for my graduate work and will send you both a current curriculum vita and letters of recommendation from my previous undergraduate research ASAP. Thank you for your time. Sincerely, Roxanne Yamashita W. Dorsey Stuart University of Hawaii Dept. of Genetics and Molecular Biology 1960 East West Rd. Biomed A104 Honolulu, Hi 96822 work: (808) 956-5516 email: dorsey@uhunix3.uhcc.hawaii.edu Doug Vann University of Hawaii Dept. of Genetics and Molecular Biology 1960 East West Rd. Biomed A104 Honolulu, Hi 96822 work: (808) 956-8878 email: dvann@uhunix3.uhcc.hawaii.edu Neil Reimer University of Hawaii BMBIT/PBRC Gilmore 411 Honolulu, Hi 96822 work: (808) 956-9653 and 956-6718 email: neil@fuchsia.uhcc.hawaii.edu From owner-mycology@net.bio.net Mon Jul 17 23:00:00 1995 Newsgroups: bionet.mycology Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!newsfeed.internetmci.com!news.sprintlink.net!tank.news.pipex.net!pipex!oleane!jussieu.fr!univ-lille1.fr!ciril.fr!news.imag.fr!univ-lyon1.fr!swidir.switch.ch!newsfeed.ACO.net!edvz.sbg.ac.at!wst.edvz.sbg.ac.at!margit From: margit@wst.edvz.sbg.ac.at (Margit Haider) Subject: Neurospora on the internet? Message-ID: Lines: 19 Sender: news@wst.edvz.sbg.ac.at (USENET News System) Reply-To: margit@wst.edvz.sbg.ac.at (Margit Haider) Organization: Univerity of Salzburg / Austria Date: Tue, 18 Jul 1995 19:54:40 GMT Dear netters, does anyone know where to find information about Neurospora sp. on the net, but not sequences but mainly about life cycle in general. I have found some references via medline search and have some info about genetics of sporulation and germination (my special interest) but there are no reviews to get a general and mor complete impression of this organism. So maybe there is some info on the net. Thanks in advance, Margit --------------------------------------------------------------------------- Margit Haider Braeuhausstr.4a | Uni Salzburg, Institut fuer Genetik und Allg. Biologie A-5020 Salzburg | Hellbrunnerstr. 34, A-5020 Salzburg Tel. 0662/825041 | Tel. 0662/8044/5750 | email: margit@dgen10.gen.sbg.ac.at --------------------------------------------------------------------------- From owner-mycology@net.bio.net Mon Jul 17 23:00:00 1995 Path: biosci!rutgers!uwm.edu!news.moneng.mei.com!news.ecn.bgu.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!Germany.EU.net!EU.net!Austria.EU.net!newsfeed.ACO.net!swidir.switch.ch!univ-lyon1.fr!ws41.cnusc.fr!ciril.fr!univ-lille1.fr!cict.fr!news From: "Philippe J. Blanc" Newsgroups: bionet.mycology Subject: WORKSHOP ON MONASCUS Date: 18 Jul 1995 15:39:26 GMT Lines: 7 Message-ID: <3ugkje$oa9@news.cict.fr> NNTP-Posting-Host: bio1.insa-tlse.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) An international workshop on the filamentous fungus MONASCUS will be organized in TOULOUSE (France) at the beginning of 1996. All mycologists colleagues can express their interest on net or on my Emailbox : blanc@insa-tlse.fr Thanks From owner-mycology@net.bio.net Mon Jul 17 23:00:00 1995 Path: biosci!rutgers!uwm.edu!news.moneng.mei.com!news.ecn.bgu.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!tank.news.pipex.net!pipex!oleane!jussieu.fr!univ-lille1.fr!cict.fr!news From: "Philippe J. Blanc" Newsgroups: bionet.mycology Subject: MEETING ON FILAMENTOUS FUNGI Date: 18 Jul 1995 15:36:45 GMT Lines: 10 Message-ID: <3ugked$oa9@news.cict.fr> NNTP-Posting-Host: bio1.insa-tlse.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Is there any meeting, conference, exhibition or workshop on filamentous fungi till the end of 95 ? I am especially interested to meet searchers working on MONASCUS Thanks to reply on net or on my Emailbox : blanc@insa-tlse.fr Thanks From owner-mycology@net.bio.net Mon Jul 17 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!tank.news.pipex.net!pipex!oleane!jussieu.fr!univ-lille1.fr!cict.fr!news From: "Philippe J. Blanc" Newsgroups: bionet.mycology Subject: MONASCUS Date: 18 Jul 1995 13:47:34 GMT Lines: 4 Message-ID: <3uge1m$n7f@news.cict.fr> NNTP-Posting-Host: bio1.insa-tlse.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Search others scientists to exchange ideas, expertise, papers and why not postdoc or trainee on MONASCUS, my main subject of interest thanks to reply either on net or on my Emailbox : blanc@insa-tlse.fr From owner-mycology@net.bio.net Mon Jul 17 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!dish.news.pipex.net!pipex!tank.news.pipex.net!pipex!oleane!jussieu.fr!univ-lille1.fr!cict.fr!news From: "Philippe J. Blanc" Newsgroups: bionet.mycology Subject: MONASCUS Date: 18 Jul 1995 13:47:18 GMT Lines: 4 Message-ID: <3uge16$n7f@news.cict.fr> NNTP-Posting-Host: bio1.insa-tlse.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: bionet Search others scientists to exchange ideas, expertise, papers and why not postdoc or trainee on MONASCUS, my main subject of interest thanks to reply either on net or on my Emailbox : blanc@insa-tlse.fr From owner-mycology@net.bio.net Mon Jul 17 23:00:00 1995 Path: biosci!rutgers!uwm.edu!msunews!harbinger.cc.monash.edu.au!bunyip.cc.uq.oz.au!news From: M.Weinert@tpp.uq.oz.au (Matthew Weinert) Newsgroups: bionet.mycology Subject: Re: Desert Truffles Date: 17 Jul 1995 22:45:48 GMT Organization: CRC for Tropical Plant Pathology Lines: 30 Message-ID: <3uep6s$8fb@dingo.cc.uq.oz.au> References: NNTP-Posting-Host: lupton.tpp.uq.edu.au X-Newsreader: WinVN 0.92.6+ In article , bpretto@io.org (Bruno Pretto & Paula Vopni) says: > > >>My next door neighbour was in Syria on an archeologigal expedition. At the >>market he was able to locate some desert truffles for me. He returned with one >>specimen weighing approx. 500 grams, for which he paid $ 10.00. It has a >>smooth outside skin unlike the french truffles. I plan to take some >>photographs of it before consuming it and I will post them on the net. >>Can anyone tell me the latin name for this truffle. > >July 16 >Have finally photographed and tasted this intriguing fungus. The exterior is >dark brown, smooth textured. The interior is beige with white interlacing >lines rather like the Italian white truffle. The flavour is very mild but >rather pleasant, texture is somewhat crunchy. There is not much aroma to >compared to the French black truffle. > >But here is a good question for all you mycologists. Is this a mychorrizal >species, and if it is what is it forming a symbiotic relationship with if >there are no trees in the desert. > Bruno Pretto, Fun Guy Farm (Toronto,Canada). > Home page http://www.io.org/~bpretto > NAMA Trustee for Toronto Mycological Society. > Secretary Oak Forest Mushroom Association (outdoor shiitake growers). I'm sure that fungi do not only form mychorrizal symbiotic relationships with trees. I'm sure that I have read somewhere that morels are thought to be mychorrizal with some types of braken ferns. The only morel cap I have found, morels don't appear to be too common in Australia, was growing in amongst some bracken. From owner-mycology@net.bio.net Mon Jul 17 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!newsfeed.internetmci.com!nntp-hub2.barrnet.net!news3.near.net!saturn.caps.maine.edu!dartvax.dartmouth.edu!usenet From: Jennifer.Loros@dartmouth.edu (Susan K. Crosthwaite) Newsgroups: bionet.mycology Subject: green fluorescent protein Date: 18 Jul 1995 16:57:18 GMT Organization: Dartmouth College, Hanover, NH Lines: 8 Sender: Susan.K.Crosthwaite@dartmouth.edu Message-ID: <3ugp5e$igc@dartvax.dartmouth.edu> NNTP-Posting-Host: at-1-sn-65.dartmouth.edu X-Posted-From: InterNews 1.0.6@dartmouth.edu Has anyone expressed green fluorescent protein in Neurospora? If so, I'd be interested to hear what promoter(s) you used and whether or not the protein is stable and easy to detect. I'm thinking about hooking up GFP to the qa-2 and ccg-2 promoters and would just like to get a feel for whether it's going to be sucessfully expressed. Please Email me if you have any information Susan.K.Crosthwaite@Dartmouth.EDU I will post a summary of the information I collect on the net. Thanks in advance, Sue Crosthwaite From owner-mycology@net.bio.net Mon Jul 17 23:00:00 1995 Path: biosci!FMPPR.FMNH.ORG!wu From: wu@FMPPR.FMNH.ORG (Qiuxin Wu) Newsgroups: bionet.mycology Subject: Roommate wanted for MSA 95 meeting Date: 18 Jul 1995 09:03:42 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <9507181604.AA07196@fmppr.fmnh.org> NNTP-Posting-Host: net.bio.net Hi! I plan to attend Mycological Society of America annual meeting which will be jointly held with AIBS in San Diego, August 6-10, 1995. I have already reserved a double-bed room in Town and Country Hotel, but my roommate decided not go to the meeting. I like to share the room and the cost ($108 per night, $54 per person per night) with someone. I am a female. If you are interested and you are a female, please send me a message to wu@fmppr.fmnh.org. Non-smoker preferred. Dr. Qiuxin Wu Phone: (312)-922-9410, ext. 718 Department of Botany Fax: (312) 427-2530 The Field Museum e-mail: wu@fmppr.fmnh.org Chicago, IL 60605-2496 From owner-mycology@net.bio.net Mon Jul 17 23:00:00 1995 Path: biosci!rutgers!uwm.edu!newsspool.doit.wisc.edu!news.doit.wisc.edu!F181-117.net.wisc.edu!tjvolk From: tjvolk@facstaff.wisc.edu (Tom Volk) Newsgroups: bionet.mycology Subject: Re: Morel mycelium Date: Tue, 18 Jul 1995 21:15:24 GMT Organization: U.S. Forest Service Lines: 30 Distribution: world Message-ID: References: <3ucei6$e4d@nexus.uiowa.edu> NNTP-Posting-Host: f181-117.net.wisc.edu In article <3ucei6$e4d@nexus.uiowa.edu> michael-clark@uiowa.edu (Michael Clark) writes: >Hello, > Sorry if this seems like a stupid question, but I've recently taken a >tissue culture from a black morel, and the mycelium looks very >different from other mushroom mycelium I've seen (C. comatus, A. brun., >S. rugoso-annulata). It is much less dense, whitish brown, and appears >to grow into the agar as well as on top of it. My media is standard >malt-extract agar supplimented with yeast extract with ampicillin >(200mg/liter of agar) added to suppress bacterial contamination. Is >this appearance normal for morel mycelium, and are there any >recommendations on agar media? Thanks. >Michael Clark >michael-clark@uiowa.edu Hi Michael. Morchella mycelium is fairly easy to recognize once you learn what it's supposed to look like. It sounds very much like the mycelium you ahve described. Fruiting morels is of course another matter entirely... It is fairly easy to grow on most kinds of medium. At least it grows on nearly every medium I've ever tried. For some additional information on recognition of the mycelium see one of my [old] morel papers: Volk, Thomas J & Thomas J Leonard. 1990 Cytology of the Life cycle of Morchella. Mycological Research. 94: 399-406 Hope this helps. Please write if you need additonal information. ---Tom Volk Center for Forest Mycology Research. Forest Products Lab. Madison Wisconsin From owner-mycology@net.bio.net Mon Jul 17 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!newsfeed.internetmci.com!news.sprintlink.net!paperboy.ids.net!ids!rand777 From: rand777@ids.net Newsgroups: bionet.mycology Subject: Re: green fluorescent protein Date: 18 Jul 95 16:08:24 EST Organization: The IDS World Network Internet Access Service Lines: 12 Message-ID: <1995Jul18.160824.1@ids> References: <3ugp5e$igc@dartvax.dartmouth.edu> NNTP-Posting-Host: ids.net In article <3ugp5e$igc@dartvax.dartmouth.edu>, Jennifer.Loros@dartmouth.edu (Susan K. Crosthwaite) writes: > Has anyone expressed green fluorescent protein in Neurospora? If so, > I'd be interested to hear what promoter(s) you used and whether or not > the protein is stable and easy to detect. I'm thinking about hooking up > GFP to the qa-2 and ccg-2 promoters and would just like to get a feel > for whether it's going to be sucessfully expressed. Please Email me if > you have any information Susan.K.Crosthwaite@Dartmouth.EDU I will post > a summary of the information I collect on the net. Thanks in advance, > Sue Crosthwaite Have you considered Pseudomonas contamination???? RRandolph From owner-mycology@net.bio.net Mon Jul 17 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.sprintlink.net!uunet!in1.uu.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: rrudine@aol.com (RRudine) Newsgroups: bionet.mycology,rec.arts.books.marketplace,rec.collecting.stamps Subject: Re: Book on mushroom stamps Date: 18 Jul 1995 19:18:26 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 2 Sender: root@newsbf02.news.aol.com Message-ID: <3uhfg2$og7@newsbf02.news.aol.com> References: <3trpfo$2q9@panix.com> Reply-To: rrudine@aol.com (RRudine) NNTP-Posting-Host: newsbf02.mail.aol.com Xref: biosci bionet.mycology:2485 rec.arts.books.marketplace:15029 rec.collecting.stamps:11274 I share Arpad's Mycophilatelic predilection and request. DOGFISHTUI From owner-mycology@net.bio.net Tue Jul 18 23:00:00 1995 Path: biosci!daresbury!trane.uninett.no!Norway.EU.net!EU.net!news.sprintlink.net!news.oz.net!news From: Marianne Elliott Newsgroups: bionet.mycology Subject: Scytalidium Date: 19 Jul 1995 01:57:43 GMT Organization: University of Washington Lines: 7 Message-ID: <3uhoqo$h5b@emerald.oz.net> NNTP-Posting-Host: conal.oz.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 32bit) I have been doing some reading about the plant pathogenic fungus Hendersonula toruloidea (Deuteromycotina, Coelomycetes), and the scytalidium state or form-genus has been mentioned. Is this the same as the genus scytalidium that is a mycoparasite on some fungi that cause rust diseases in trees? I guess I'm confused about what is meant by form-genus. Any information would be appreciated. ----Marianne From owner-mycology@net.bio.net Tue Jul 18 23:00:00 1995 Path: biosci!bcm!news.msfc.nasa.gov!newsfeed.internetmci.com!lamarck.sura.net!news.uky.edu!chuck From: Chuck Staben Newsgroups: bionet.mycology Subject: Re: Neurospora on the internet? Date: 19 Jul 1995 00:05:55 GMT Organization: University of Kentucky Lines: 6 Message-ID: <3uhi93$pj9@service1.uky.edu> References: NNTP-Posting-Host: bs6.mi.uky.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Sporulation and the relationship of sporulation pathways to one another was reviewed recently by Springer. Germination of conidia has not been very active recently. You might look under S. Brody for older reviews. Germination of plant pathogenic spores is of considerable current interest. From owner-mycology@net.bio.net Tue Jul 18 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!quagga.ru.ac.za!ucthpx!news From: "Gonzalo H. Aguilar" Newsgroups: bionet.mycology Subject: Information on Shiitake Mushroom Date: 19 Jul 1995 14:56:02 GMT Organization: Botany Department, University of Cape Town Lines: 13 Message-ID: <3uj6e2$ijg@ucthpx.uct.ac.za> NNTP-Posting-Host: 137.158.133.78 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) I'm in South Africa and would like to obtain shiitake mushrooms (Lat. Name: Lentinula edodes (Berkeley)). Anybody there with tips on where and how to get them. Thanks in advance Gonzalo H. Aguilar Tel: 021 6502436 Department of Botany e-mail: GAGUILAR@botzoo.uct.ac.za University of Cape Town : GAGUILAR@uctvax.uct.ac.za ========================== oo0 - 0oo =============================== From owner-mycology@net.bio.net Tue Jul 18 23:00:00 1995 Path: biosci!daresbury!trane.uninett.no!nac.no!Norway.EU.net!EU.net!news.sprintlink.net!tank.news.pipex.net!pipex!news.uoregon.edu!news.u.washington.edu!netnews.nwnet.net!reuter.cse.ogi.edu!engr.orst.edu!news.PEAK.ORG!microbe From: microbe@PEAK.ORG (Steven Carpenter) Newsgroups: bionet.mycology Subject: Penicillium Book Wanted Date: 18 Jul 1995 23:51:53 GMT Organization: CS Outreach Services, Oregon State University, Corvallis, OR, USA Lines: 15 Message-ID: <3uhhep$kp@odo.PEAK.ORG> NNTP-Posting-Host: kira X-Newsreader: TIN [version 1.2 PL2] I would like to buy a copy of "A Manual of the Penicillia" by Raper, Thom and Fennel, 1949. If you have one for sale or know where I could obtain one, please e-mail me at: microbe@peak.org Thanks. -Steve Carpenter Cascade Research Associates & Abbey Lane Laboratory From owner-mycology@net.bio.net Tue Jul 18 23:00:00 1995 Path: biosci!daresbury!trane.uninett.no!Norway.EU.net!EU.net!news.sprintlink.net!uunet!in1.uu.net!news.uiowa.edu!not-for-mail From: dgaunt@uhl.uiowa.edu (Dennis Gaunt) Newsgroups: bionet.mycology Subject: Re: Scytalidium Date: 19 Jul 1995 20:05:36 GMT Organization: University of Iowa, Iowa City, IA, USA Lines: 20 Distribution: world Message-ID: <3ujoig$8ku@nexus.uiowa.edu> References: <3uhoqo$h5b@emerald.oz.net> NNTP-Posting-Host: franklin.uhl.uiowa.edu X-Newsreader: TIN [UNIX 1.3 950515BETA PL0] Marianne Elliott (conal@oz.net) wrote: : I have been doing some reading about the plant pathogenic fungus : Hendersonula toruloidea (Deuteromycotina, Coelomycetes), and the : scytalidium state or form-genus has been mentioned. Is this the same as : the genus scytalidium that is a mycoparasite on some fungi that cause : rust diseases in trees? I guess I'm confused about what is meant by : form-genus. Any information would be appreciated. ----Marianne : A form-genus is commonly used for the anamorph or conidial state of the fungus. The prefix form- denotes that these groups don't imply phylogenetic relationships. They're simply "handles" for use in discussing the various forms. -- ---------------------------------------------------------------------- | Dennis D. Gaunt | Internet: dgaunt@uhl.uiowa.edu | | The University of Iowa | Voice: (319) 335-4500 | | Oakdale Research Campus | FAX: (319) 335-4555 | | Iowa City, Iowa 52242 | | ---------------------------------------------------------------------- From owner-mycology@net.bio.net Tue Jul 18 23:00:00 1995 Newsgroups: bionet.mycology Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!btnet!gold.compulink.co.uk!cix.compulink.co.uk!usenet From: ecoli@cix.compulink.co.uk ("K N and P J Harris") Subject: Fungus ? in deep sediment Message-ID: Organization: Compulink Information eXchange X-Newsreader: WigWam Date: Wed, 19 Jul 1995 07:33:23 GMT Lines: 19 Student brought in a sample of calcareous sediment from the Barcelona area in Spain. Question ? What are the little black spots ? Low power stereo (x40) revealed many more or less spherical black structures, half submerged in the soft rock base. About 200 to 300 microns in diameter, some with a small pore at the top. Poking with a scalpel showed many of them to be fragile with the consistency of soft charcoal and hollow. One more resilient specimen showed evidence of a hard gelatinous content. Eventually a few were observed to have small gelatinous, ochre coloured finger like projections, often curled, poking out of the top of the structure. Still only about 200 to 400 microns long. We managed to photograph a couple of the specimens. As a humble soil microbiologist, used to Penicillium, Aspergillus and Trichoderma with the odd Mucor thrown in for fun, I have never seen their like before. I suspect some type of ascomycete on size grounds but would welcome ANY suggestions. Peter Harris, Soil Science, University of Reading, UK. AKA From owner-mycology@net.bio.net Tue Jul 18 23:00:00 1995 Path: biosci!SPRINT.UCCS.EDU!jmattoon From: jmattoon@SPRINT.UCCS.EDU (James R Mattoon) Newsgroups: bionet.mycology Subject: (none) Date: 19 Jul 1995 16:17:47 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net How do I access this newsgroup from unix? jmattoon@sprint.uccs.edu From owner-mycology@net.bio.net Wed Jul 19 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!sdd.hp.com!caen!reeve.research.aa.wl.com!decwrl!pagesat.net!netserv.com!news2.aimnet.com!usenet From: xyzzyx@aimnet.com (xyzzyx) Newsgroups: bionet.mycology Subject: Re: Morel mycelium Date: 20 Jul 1995 05:47:34 GMT Organization: xyzzyx consultants Lines: 33 Distribution: world Message-ID: <3ukqlm$oh5@news2.aimnet.com> References: <3ucei6$e4d@nexus.uiowa.edu> NNTP-Posting-Host: dial-bel1-3.iway.aimnet.com Mime-Version: 1.0 Content-Type: Text/Plain; charset=ISO-8859-1 X-Newsreader: WinVN 0.99.5 In article <3ucei6$e4d@nexus.uiowa.edu>, michael-clark@uiowa.edu says... > >Hello, > Sorry if this seems like a stupid question, but I've recently taken a >tissue culture from a black morel, and the mycelium looks very >different from other mushroom mycelium I've seen (C. comatus, A. brun., >S. rugoso-annulata). It is much less dense, whitish brown, and appears >to grow into the agar as well as on top of it. My media is standard >malt-extract agar supplimented with yeast extract with ampicillin >(200mg/liter of agar) added to suppress bacterial contamination. Is >this appearance normal for morel mycelium, and are there any >recommendations on agar media? Thanks. > >Michael Clark >michael-clark@uiowa.edu Hi Michael, your description s