From owner-mycology@net.bio.net Fri Dec 01 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!vixen.cso.uiuc.edu!uwm.edu!reuter.cse.ogi.edu!engr.orst.edu!news.PEAK.ORG!PEAK.ORG!ddelace From: Dave Delacey Newsgroups: bionet.mycology Subject: Thanks for the recommendations! Date: Thu, 30 Nov 1995 18:49:27 -0800 Organization: CS Outreach Services--PEAK, Corvallis, Oregon, USA Lines: 11 Message-ID: NNTP-Posting-Host: peak.org Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Arora's it will be, then; it was unanimous. I'm sure I will eventually get a copy of "The Savory Wild Mushroom" as well, but I AM new at this and not at all comfortable with my ID'ing skills. I'll stick to morels for now |) Thanks again, Dave DeLacey Corvallis, Or. From owner-mycology@net.bio.net Fri Dec 01 22:00:00 1995 Path: biosci!bloom-beacon.mit.edu!newsfeed.internetmci.com!info.ucla.edu!library.ucla.edu!csulb.edu!drivel.ics.uci.edu!news.service.uci.edu!gandalf.bio.uci.edu!rdlab From: rdlab@gandalf.bio.uci.edu (rlab davis) Newsgroups: bionet.mycology Subject: in vitro transcription using N. crassa nuclear extracts? Date: Fri, 1 Dec 1995 14:51:00 Organization: Bio Sci Lines: 25 Message-ID: NNTP-Posting-Host: davislab3.bio.uci.edu X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] I am attempting to determine if changes in the abundance of a particular N. crassa mRNA under two different growth conditions are due changes in the cytoplasmic stability of the message. As a control I would like to determine if there are any differences in transcription of the gene in the two growth conditions. (Nuclear run-on assays are messy owing to the normally low level of expression of this gene which leads to high signal to noise ratios.) Brett Tyler and Norman Giles have published a protocol for making crude RNAPII exctracts using conidia. My concern is that if the stability of my mRNA of interest is regulated by a cytoplasmic factor, it might be present in these extracts. Is this a valid concern? The solution to this problem would seem to be to use nuclear extracts for the in vitro assays. Has anyone ever made a nuclear RNAP II transcription extract? (I didn't find one in a search of current literature) Could I use one of the nuclear isolition procedures for Neurospora and then modify the Tyler and Giles protocol? Or, could I obtain extracts from isolated nuclei using a mammalian or yeast protocol? I would appreciate any feedback/help I can get. Thanks in advance, Martin Hoyt (Rowland Davis lab) mahoyt@uci.edu Dept. Mol. Biol. and Biochem. Univ. of Calif., Irvine From owner-mycology@net.bio.net Fri Dec 01 22:00:00 1995 Path: biosci!UCDAVIS.EDU!bmtyler From: bmtyler@UCDAVIS.EDU (Brett Tyler) Newsgroups: bionet.mycology Subject: Re: Neurospora in vitro transcription Date: 1 Dec 1995 17:53:49 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 74 Sender: daemon@net.bio.net Distribution: world Message-ID: <199512020152.RAA27243@dale.ucdavis.edu> NNTP-Posting-Host: net.bio.net Dear Martin, I tend to agree with Rowland that in vitro transcription reactions are NOT the way to, even nuclear ones, to demonstrate a lack of regulation. Even the best extracts tend to be missing various transcription factors, because you are trying to isolate so many different proteins all at once. So if you got a negative result, you would not know if you had just lost the relevant factor from the extract, especially if you used nuclei, which are leaky at the best of times. We have tried nuclear extracts with poor success using just the approach you were thinking of. One way to test your hypothesis is to connect the spe-1 promoter to a reporter such as qa-2 or something and show that that transcript is not regulated. Then connect another promoter to the spe-1 mRNA coding region, and show that this IS regulated by polyamines. There is the question of whether you have an internal transcriptional regulatory sequence, however a way to get around this might be to drive the spe-1 mRNA off the T7 RNA polymerase promoter, which will be insensitive to eukaryotic regulatory sequences. The T7 polymerase would be produce off another promoter. Kevin Struhl did this in yeast several yearsago. Ref is: Chen W; Tabor S; Struhl K. Distinguishing between mechanisms of eukaryotic transcriptional activation with bacteriophage T7 RNA polymerase. Cell, 1987 Sep 25, 50(7):1047-55. Hope this helps Brett Tyler >Brett, > >I am a current grad student of Rowland Davis, and he suggested I write to >you to see if you could offer us any advice. We are currently studying how >spe-1 mRNA (encoding ornithine decarboxylase) abundance is repressed by >polyamines (the products of the ODC reaction). Our preliminary evidience >suggests that the stability of spe-1 mRNA is reduced by polyamines and that >that changes in spe-1 stability of normal and polyamine-starved cultures can >account for the changes in spe-1 mRNA abundance in those cultures. > >We wish to verify that the contribution of spe-1 transcriptional regulation >is minor, if present at all. We have done nuclear run-on experiments, but we >get poor signal to noise ratios due to the normally low abundance of spe-1 >message. I am now considering measuring differences in spe-1 transcription >under the two conditions (+/- polyamines) using an in vitro system. > >I have read your 1985 PNAS paper, but I am concerned that a cytoplasmic >factor responsible for spe-1 turnover may persist in the whole-cell extracts. >Is this a legitamate concern? Could this be overcome using RNase inhibitors? >A solution to this problem would be to carry out the transcription reactions >using nuclear extracts. I cannot find any references to such a technique. >Could your whole-cell protocol be easily modified for use with isolated >nuclei? I am afraid that I know very little about the technical aspects of >in vitro transcription, so I would like any advice you can offer on how to >proceed. > >Rowland would like some assurance on how well any in vitro technique will >work, since we seek negative evidence from the data (no change in >transcription). Again, any feedback you could give would be invaluble to us. > >Thank you for your time, > >Martin Hoyt >Dept. Mol. Biol. and Biochem. >Univ. of Calif., Irvine **************** Brett Tyler Professor Department of Plant Pathology University of California, Davis bmtyler@ucdavis.edu **************** From owner-mycology@net.bio.net Fri Dec 01 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: Articles on primates eating mushrooms ??? Date: 2 Dec 1995 16:33:29 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 5 Sender: root@newsbf02.news.aol.com Message-ID: <49qgn9$oqm@newsbf02.news.aol.com> Reply-To: stamets1@aol.com (Stamets 1) NNTP-Posting-Host: newsbf02.mail.aol.com I am searching for any articles which describes primates eating mushrooms. Does anyone know of any citations ? On the 'net? Thanks in advance. paul stamets From owner-mycology@net.bio.net Fri Dec 01 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!prodigy.com!usenet From: ZUBP40A@prodigy.com (David Pinero) Newsgroups: bionet.mycology Subject: Geotrichum vs. Arthroconidial Basidiomycet Date: 2 Dec 1995 02:55:06 GMT Organization: Prodigy Services Company 1-800-PRODIGY Lines: 6 Distribution: world Message-ID: <49of6a$1sbe@usenetw1.news.prodigy.com> NNTP-Posting-Host: inugap5.news.prodigy.com X-Newsreader: Version 1.2 I have some confusion over somewhat fluffy isolates matching the Geothrichum form. Is it correct to call them Geothrichum? Is Geotrichum a term for the imperfect state of more than one species, including basidiomycetes? Or does it comprise only one species? Literature about this is highly contradictory. From owner-mycology@net.bio.net Sat Dec 02 22:00:00 1995 Path: biosci!agate!news.Stanford.EDU!usenet From: Matt Springer Newsgroups: bionet.mycology Subject: Re: Articles on primates eating mushrooms ??? Date: 3 Dec 1995 09:34:48 GMT Organization: Stanford University Lines: 6 Message-ID: <49rqvo$h57@nntp.Stanford.EDU> References: <49qgn9$oqm@newsbf02.news.aol.com> NNTP-Posting-Host: r312-pb540c.stanford.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) X-URL: news:49qgn9$oqm@newsbf02.news.aol.com Are we talking about the primates with short hair all over their bodies or the primates with long hair on the top of their head? ;-) From owner-mycology@net.bio.net Sat Dec 02 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!miwok!bdt.com!usenet From: mwood@bdt.com (Michael Wood) Newsgroups: bionet.mycology Subject: Re: Any Mycology groups in SoCal? Date: Sun, 03 Dec 1995 16:47:06 GMT Organization: Paradigm Micro Systems Lines: 28 Message-ID: <49skec$p1b@bdt.bdt.com> References: <49lp88$g90@gap.cco.caltech.edu> NNTP-Posting-Host: mwood.dial-up.bdt.com X-Newsreader: Forte Agent .99a.107 bwoods@alumni.caltech.edu (Bradley B. Woods) wrote: >Hi, I realize it ain't the best place to go looking for mushrooms, but it >is where I live, so are there any amateur mycology groups in southern >California? > I have a list of 90-100 amateur mushroom clubs in North America at Myko Web: http://www.bdt.com/home/mwood/mycology.html From that list: Los Angeles Mycological Society (LAMS) 23611 Golden Springs Drive #I-3 Diamond Bar, CA 91765 You can read past issues of the LAMS newsletter "Spore Prints" at: gopher://gopher.igc.apc.org:70/11/pubs/sporeprint Happy hunting! | Michael Wood Paradigm Micro Systems | | mwood@bdt.com San Leandro, CA USA | | | From owner-mycology@net.bio.net Sat Dec 02 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!EU.net!sun4nl!news.omroep.nl!Utrecht.NL.net!rubens.telebyte.nl!news From: "Peter Oei (ECO Consult)" Newsgroups: bionet.mycology Subject: World production figures of edible mushrooms Date: 3 Dec 1995 20:11:55 GMT Organization: ECO Lines: 9 Message-ID: <49t0ac$2tp@rubens.telebyte.nl> NNTP-Posting-Host: nm-1-22.telebyte.nl Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Who can provide me with more or less reliable data how many mushrooms have been produced and which species, later than 1991? I have a list from 1991 by Shu Ting Chang, have there been more recent publications which include speciality mushrooms like Pholiota nameko and heticium erniaceus? Peter Oei From owner-mycology@net.bio.net Sat Dec 02 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!in2.uu.net!news.deltanet.com!usenet From: cardpro@deltanet.com (Charles West) Newsgroups: bionet.mycology Subject: Re: Any Mycology groups in SoCal? Date: Sun, 03 Dec 1995 18:13:45 GMT Organization: Delta Internet Services, Anaheim, CA Lines: 12 Message-ID: <49sljr$3j3@news1.deltanet.com> References: <49lp88$g90@gap.cco.caltech.edu> NNTP-Posting-Host: ana1035.deltanet.com X-Newsreader: Forte Free Agent 1.0.82 bwoods@alumni.caltech.edu (Bradley B. Woods) wrote: >Hi, I realize it ain't the best place to go looking for mushrooms, but it >is where I live, so are there any amateur mycology groups in southern >California? >B Try Spore Print via Eco Net http://www.econet.apc.org/igc/www.myco.html From owner-mycology@net.bio.net Sun Dec 03 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!vixen.cso.uiuc.edu!news.ksu.ksu.edu!news.cis.okstate.edu!news.ecn.uoknor.edu!news.uoknor.edu!pdlab2.noble.org!user From: library@noble.org (Library) Newsgroups: bionet.mycology Subject: Re: Q: can two fungal species mate and reproduce new species? Date: 3 Dec 1995 22:58:50 GMT Organization: The Noble Foundation Lines: 70 Message-ID: References: NNTP-Posting-Host: pdlab2.noble.org In article , until@teleport.com wrote: > I've been told by a horticulturist that it's possible for two > different fungal species to mate and reproduce a third > species. Is this really possible? What would be the > limitations--as in, would the two species have to be closely > related? Would the offspring fungus be able to reproduce > itself--or would it be sterile? What kind of spore-dispersal > mechanisms would be dominant and passed on to the > offspring fungus in relation to other spore-dispersal mechanisms? > > In the two books (_not_ ID books) I have about fungi and their > spores, nothing like this is mentioned. So, is this completely > speculative, or is this possible? > > - Tara K. Harper > until@teleport.com > > / Even a thought / > / Even a possibility can shatter us and transform us / > / - Nietzsche / Dear Tara Harper; It is possible for two different fungal species to mate, but only between closely related species in which mating genes and intersterility genes are compatible (Otrosina et al. 1993). I am familiar to only one fungal species. Let me tell it first. Wood rot fungal species Heterobasidion annosum (basidiomycete) can be divided into a number of sub-species with different host tree preference. It is said that speciation (formation of new species) is undergoing within this fungus, since between races they don't usually mate. The European Fir (Eur-F) strain and the European spruce (Eur-S) are intersterile to each other, although they are genetically very closely related (Kasuga et al. 1993). On the other hand, the North American spruce (NAm-S) strain can mate with the Eur-F or Eur-S strains, although the NAm-S strain is genetically distant from both Eur-S and Eur-F strains. This is a vary interesting system for fungal speciation (Otrosina et al 1993). It is claimed that recently, Heterobasidion annosum in Europe gained host specificity on spruce and fir , and simultaneously intersterility system was developed between spruce and fir specialised groups. A hybrid between them may be less pathogenic, and could be at a disadvantage in nature. This phenomenon has been reported for several other plant pathogenic fungi. On the other hand, American and European strains have not developed interstrility system, since they are geographically separated....no need to worry about hybrid formation. Now I can give you some hints. When new species evolved within a population, it should become sterile to the original species to maintain new gene pool. It is therefore unlikely that two different fungal species can mate to form a new species in nature. However, if geographically isolated two closely related species meet, they may form a hybrid. In Heterobasidion system, hybrids are fertile and segregation of intersterility genes have been studied. However hybrids tend to revert back to parent genotype. Otrosina WJ et al. (1993). Can. J. Bot 71, 1064-1071 Kasuga T et al. (1993) Curr Genet 24 433-436 Hope you've got some idea about fungal hybrids. Ciao, Takao Kasuga The Noble Foundation Ardmore OK 73401 POBox 2180 From owner-mycology@net.bio.net Sun Dec 03 22:00:00 1995 Path: biosci!utmb.edu!lester.pasarell From: lester.pasarell@utmb.edu Newsgroups: bionet.mycology Subject: Re: Geotrichum vs. Arthroconidial Basidiomycet Date: 4 Dec 1995 08:40:02 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 56 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <49of6a$1sbe@usenetw1.news.prodigy.com> Reply-To: lester.pasarell@utmb.edu NNTP-Posting-Host: net.bio.net On Sat, 2 Dec 1995, David Pinero wrote: > I have some confusion over somewhat fluffy isolates matching the > Geothrichum form. Is it correct to call them Geothrichum? Is Geotrichum a > term for the imperfect state of more than one species, including > basidiomycetes? Or does it comprise only one species? Literature about > this is highly contradictory. > > > Just my opinion...but it may be nice to hear others people comments about this one.... Yes..the literature is very contradictory...it depends basically in which part of the ocean you live...(-: and what books you follow...but Usually the basidiomycete Arthroconidial forms are grouped around Trichosporon sp. most of them having a positive urea reaction were as Geotricum sp. is related to ascomycetes and has a Urea negative reaction...... Most commercial yeast identifications methods include this two genera in their data bases, so in theory if you have one of the common ones you should be able to identify it with a biochemical strip and a morphology plate (Dalmau method) Another genus that is fluffy , white and produces Arthroconidia...and its more common in some places than Trichosporon and Geotricum is Arthrographis sp.. This one tends to be fluffy all the time and does not tend to have a yeast like apearance in culture or when you take a look under the microscope. There is also some Basidios that will produce arthroconidia...but they will also have clamp connections in the hyphae. There is a bunch of other white fluffy moulds with arthroconidia... that you may see more often depending of what you are working with. By the way....were did you isolate this one from? hope this helps..let me know if you need more information Lester **************************************************************************** Lester Pasarell-Luiggi Lester.Pasarell@utmb.edu Medical Mycology Research laboratory LPASAREL@beach.utmb.edu University of Texas Medical Branch Galveston, Texas http://fungus.utmb.edu furuki o tazune atarashiki o shiru Study the old to understand the new --- Japanese Proberb----- ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ From owner-mycology@net.bio.net Sun Dec 03 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!EU.net!sun4nl!Utrecht.NL.net!rubens.telebyte.nl!news From: "Peter Oei (ECO Consult)" Newsgroups: bionet.mycology Subject: Re: Q: can two fungal species mate and reproduce new species? Date: 4 Dec 1995 15:50:49 GMT Organization: ECO Lines: 6 Message-ID: <49v5cp$rhr@rubens.telebyte.nl> References: NNTP-Posting-Host: nm-1-15.telebyte.nl Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) There are more species which can be distinguished but which are closely related and can form fertile ofspring, e.g. Pleurotus abalonus and P. cystidiosus Peter Oei From owner-mycology@net.bio.net Sun Dec 03 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: Re: Articles on primates eating mushrooms ??? Date: 4 Dec 1995 10:16:06 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 2 Sender: root@newsbf02.news.aol.com Message-ID: <49v3bm$h6o@newsbf02.news.aol.com> References: <49rqvo$h57@nntp.Stanford.EDU> Reply-To: stamets1@aol.com (Stamets 1) NNTP-Posting-Host: newsbf02.mail.aol.com All right, let's say non-human primates! From owner-mycology@net.bio.net Sun Dec 03 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!usc!newshub.csu.net!axe.humboldt.edu!cws1 From: cws1@axe.humboldt.edu Newsgroups: bionet.mycology Subject: Request for help with hyphomycete id. Date: Sun, 3 Dec 1995 19:47:01 -0800 Organization: Information Resources and Technology Lines: 27 Message-ID: References: <49t0ac$2tp@rubens.telebyte.nl> NNTP-Posting-Host: axe.humboldt.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII In-Reply-To: <49t0ac$2tp@rubens.telebyte.nl> I've got a real headache of an isolate from Carex roots gathered from alpine and subalpine meadows. If you might be able to offer suggestions (it's not in Domsch and Gams) it has the following properties: - clamp connections at every septa, extending about 2um from the hypha - hypha about 1.6 - 4.5 um diameter - cross connections frequent between hypha 30+ um apart. - connidia found scattered about in small clusters, but no recognizeable conideogenous cells; conidia abo9ut1.7 - 3.6 um diameter - culture is powdery white. - meadow soils were likely Histisols. Any ideas? Thanks in advance! Curt Seeliger cws1@axe.humboldt.edu Humbled State University Arcata, CA 95521 The views expressed herein are "I can not live without books." not only my views, but also the -- Thomas Jefferson views of Humboldt State University and the rest of the world as well, "I don't want to live in a country so wise up bucko! that can't make it's own television sets" -- H. Ross Perot From owner-mycology@net.bio.net Sun Dec 03 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!news.cdsnet.net!usenet From: Jerry Haugen Newsgroups: bionet.mycology Subject: Re: World production figures of edible mushrooms Date: 4 Dec 1995 01:50:44 GMT Organization: The Mushroom Company Lines: 25 Message-ID: <49tk5k$rdd@news.cdsnet.net> References: <49t0ac$2tp@rubens.telebyte.nl> NNTP-Posting-Host: kf-pm1-027.cdsnet.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.12(Macintosh; I; PPC) X-URL: news:49t0ac$2tp@rubens.telebyte.nl "Peter Oei (ECO Consult)" wrote: >Who can provide me with more or less reliable data how many mushrooms >have been produced and which species, later than 1991? > >I have a list from 1991 by Shu Ting Chang, have there been more recent >publications which include speciality mushrooms like Pholiota nameko and >heticium erniaceus? > >Peter Oei > Peter, There is a lot of world-wide data at: gopher://mann77.mannlib.cornell.edu:70/11/data-sets/specialty/94003 Unfortunately, not much info by species. If you find the data you are looking for, could you post it to this newsgroup? Thanks. -Jerry- The Mushroom Growers' Newsletter MycoWrld@cdsnet.net From owner-mycology@net.bio.net Sun Dec 03 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!news.u.washington.edu!homer29.u.washington.edu!todell From: Thomas O'Dell Newsgroups: bionet.mycology Subject: Re: Q: can two fungal species mate and reproduce new species? Date: Mon, 4 Dec 1995 08:45:29 -0800 Organization: University of Washington Lines: 84 Message-ID: References: NNTP-Posting-Host: homer29.u.washington.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII NNTP-Posting-User: todell In-Reply-To: Of some relevance is recent work demonstrating the ability to produce intergeneric hybrids using protoplasts. see Ogawa, K. 1993. Interspecific and intergeneric hybridization of edible mushrooms by protoplast fusion. pp. 193-206 in Genetics and Breeding of Edible mushrooms. Chang, S-T et al. eds. Gordon and Breach Science Publishers. On 3 Dec 1995, Library wrote: > In article , until@teleport.com wrote: > > > I've been told by a horticulturist that it's possible for two > > different fungal species to mate and reproduce a third > > species. Is this really possible? What would be the > > limitations--as in, would the two species have to be closely > > related? Would the offspring fungus be able to reproduce > > itself--or would it be sterile? What kind of spore-dispersal > > mechanisms would be dominant and passed on to the > > offspring fungus in relation to other spore-dispersal mechanisms? > > > > In the two books (_not_ ID books) I have about fungi and their > > spores, nothing like this is mentioned. So, is this completely > > speculative, or is this possible? > > > > - Tara K. Harper > > until@teleport.com > > > > / Even a thought / > > / Even a possibility can shatter us and transform us / > > / - Nietzsche / > > Dear Tara Harper; > > It is possible for two different fungal species to mate, but only between > closely related species in which mating genes and intersterility genes are > compatible (Otrosina et al. 1993). I am familiar to only one fungal > species. Let me tell it first. > > Wood rot fungal species Heterobasidion annosum (basidiomycete) can be > divided into a number of sub-species with different host tree preference. > It is said that speciation (formation of new species) is undergoing within > this fungus, since between races they don't usually mate. The European > Fir (Eur-F) strain and the European spruce (Eur-S) are intersterile to > each other, although they are genetically very closely related (Kasuga et > al. 1993). On the other hand, the North American spruce (NAm-S) strain > can mate with the Eur-F or Eur-S strains, although the NAm-S strain is > genetically distant from both Eur-S and Eur-F strains. > > This is a vary interesting system for fungal speciation (Otrosina et al > 1993). It is claimed that recently, Heterobasidion annosum in Europe > gained host specificity on spruce and fir , and simultaneously > intersterility system was developed between spruce and fir specialised > groups. A hybrid between them may be less pathogenic, and could be at a > disadvantage in nature. This phenomenon has been reported for several > other plant pathogenic fungi. On the other hand, American and European > strains have not developed interstrility system, since they are > geographically separated....no need to worry about hybrid formation. > > Now I can give you some hints. When new species evolved within a > population, it should become sterile to the original species to maintain > new gene pool. It is therefore unlikely that two different fungal species > can mate to form a new species in nature. However, if geographically > isolated two closely related species meet, they may form a hybrid. In > Heterobasidion system, hybrids are fertile and segregation of > intersterility genes have been studied. However hybrids tend to revert > back to parent genotype. > > > Otrosina WJ et al. (1993). Can. J. Bot 71, 1064-1071 > Kasuga T et al. (1993) Curr Genet 24 433-436 > > Hope you've got some idea about fungal hybrids. > > Ciao, > > Takao Kasuga > The Noble Foundation > Ardmore OK 73401 > POBox 2180 > > todell@u.washington.edu Mycology is better than Urology! From owner-mycology@net.bio.net Sun Dec 03 22:00:00 1995 Path: biosci!agate!news.ucdavis.edu!library.ucla.edu!info.ucla.edu!newsfeed.internetmci.com!in1.uu.net!news.u.washington.edu!homer29.u.washington.edu!todell From: Thomas O'Dell Newsgroups: bionet.mycology Subject: Re: Does mushroom picking damage or encourage the Mycelium? Date: Mon, 4 Dec 1995 08:39:37 -0800 Organization: University of Washington Lines: 64 Message-ID: References: <4826hj$ehe@alpha.aladdin.co.uk> <4906lu$sha@nic-nac.CSU.net> NNTP-Posting-Host: homer29.u.washington.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII NNTP-Posting-User: todell In-Reply-To: <4906lu$sha@nic-nac.CSU.net> You seem to want to apply the clear cut mentality to all natural resources. National Forests are required to manage their resources on a sustained yield basis. Since they don't know what that is for fungi it is appropriate to err on the side of caution, rather than the mistakes of the timber (over-) harvest. If public lland managers are changing their attitudes regarding careful husbanding of resources, its about time! My impression is not so much a change in attitude as a response to the successful lawsuits re. long-term mismanagement of naturla resources on public lands. The paucity of data on effects of mushroom collection put managers in the position of shooting in the dark. Until they have relaible data on which to base decisions they are justified in keeping impacts to a minimum. By the way, National Forest Regulations also require charging "fair market value" for harvested products, users of the forest drive on federally built roads and receive other services that are expensive. Only recently has there been any discussion of user fees for harvesting mushrooms. Most mushroom harvest is actually theft. What are you willing to pay in return for this use of public lands? On 22 Nov 1995, Steve Pencall wrote: > > Apropos of this subject, I have prepared a summary of my observations over > a 9 year period at a chanterelle collecting site in coastal central > California. It is NOT a controlled scientific study, but I believe that > the nature of the site and of my collection patterns make it relevant to > this topic. It is available by e-mail by sending a request to me at the > address at the bottom of this post. The summary is approximately 10K of > ASCII text. Please put "chanterelle summary" in the subject line. > > Your post of Nov. 11 touched on an important but controversial subject. > We here in the US have faced a proliferation of regulations by various > public land management agencies designed to curtail and often eradicate > ALL mushroom collecting EXCEPT by scientific permit. The ostensible > reason for these policies is a concern that the viability of edible > mushroom populations may be threatened by collecting; commercial, amateur, > or both. The scientific premise of such policies is flimsy to say the > least, falling principally into the "could be," "might be," "possibly > harmful" categories. Although the few available studies indicate little > or no harmful effect from MODERATE levels of collecting using low impact > collection methods, as indicated by Lorelei Norvell's post of Nov. 11, > these studies have been little heeded by the advocates of more > restrictions, both in and out of government, because they do not fit into > the ideological mindset of these people. > > A reaction to commercial collecting is only partly responsible for the > increase in restrictions. Much more insidious is an attitude shift on the > part of many land managers which regards removing anything from the > natural environment as a violation of Nature. Many of them seem to have snip > Steven Pencall > > Editor, The Spore Print > Journal of The Los Angeles Mycological Society > > "Leave the beaten path and dive into the woods" > --Alexander Graham Bell > > > todell@u.washington.edu Mycology is better than Urology! From owner-mycology@net.bio.net Mon Dec 04 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: brianmc@aol.com (BrianMc) Newsgroups: bionet.mycology Subject: Re: Pacific NW field guides? Date: 4 Dec 1995 21:42:26 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 25 Sender: root@newsbf02.news.aol.com Message-ID: <4a0bii$3oa@newsbf02.news.aol.com> References: <49o22u$lj8@galaxy.ucr.edu> Reply-To: brianmc@aol.com (BrianMc) NNTP-Posting-Host: newsbf02.mail.aol.com Specifc to Oregon, Washington, Idaho, British Columbia and Western Montana, are a set of keys produced by the Pacific Northwest Key Council. Pacific Northwest Key Council 1943 SE Locust Ave. Portland, OR 97201-2250 These are keys to specific genera, and some are still in the trial stage. Others may not be available, as they're in the process of being updated. However the prices are right. The Pacific Northwest Key Council is a non-profit organization, and produces the keys at cost. A complete set will turn out heavier than Arora's "Mushrooms Demystified"! I specifically recommend Ben Woo's key to Russula, which is a very detailed treatment, and Coleman Luethy's Lactarius key. Both are fairly recent (more so than Arora, which is desperately in need of a new edition), and more detailed that a more generalized guide. --Brian McNett ---------------------------------------------------------- | Brian McNett e-mail: | Editor: MycoInfo | Submissions: | Promoting Mycology in the Online Community From owner-mycology@net.bio.net Mon Dec 04 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!swrinde!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: brianmc@aol.com (BrianMc) Newsgroups: bionet.mycology Subject: Re: Pacific NW field guides? Date: 4 Dec 1995 21:22:59 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 25 Sender: root@newsbf02.news.aol.com Message-ID: <4a0ae3$36q@newsbf02.news.aol.com> References: <49o22u$lj8@galaxy.ucr.edu> Reply-To: brianmc@aol.com (BrianMc) NNTP-Posting-Host: newsbf02.mail.aol.com Specifc to Oregon, Washington, Idaho, British Columbia and Western Montana, are a set of keys produced by the Pacific Northwest Key Council. Pacific Northwest Key Council 1943 SE Locust Ave. Portland, OR 97201-2250 These are keys to specific genera, and some are still in the trial stage. Others may not be available, as they're in the process of being updated. However the prices are right. The Pacific Northwest Key Council is a non-profit organization, and produces the keys at cost. A complete set will turn out heavier than Arora's "Mushrooms Demystified"! I specifically recommend Ben Woo's key to Russula, which is a very detailed treatment, and Coleman Luethy's Lactarius key. Both are fairly recent (more so than Arora, which is desperately in need of a new edition), and more detailed that a more generalized guide. --Brian McNett ---------------------------------------------------------- | Brian McNett e-mail: | Editor: MycoInfo | Submissions: | Promoting Mycology in the Online Community From owner-mycology@net.bio.net Mon Dec 04 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!uwm.edu!reuter.cse.ogi.edu!engr.orst.edu!news.PEAK.ORG!microbe From: microbe@PEAK.ORG (Steven Carpenter) Newsgroups: bionet.mycology Subject: Re: Request for help with hyphomycete id. Date: 4 Dec 1995 14:08:30 GMT Organization: CS Outreach Services, Oregon State University, Corvallis, OR, USA Lines: 43 Message-ID: <49uvcu$cdb@odo.PEAK.ORG> References: <49t0ac$2tp@rubens.telebyte.nl> NNTP-Posting-Host: peak.org X-Newsreader: TIN [version 1.2 PL2] Your description is interesting, but identification of fungi over the internet is pretty difficult unless you can post a visual with the short verbal description. Dr. David Largent is a mycologist at your university, and could probably figure this out for you. If not, my company will do so for a fee. -Steven E. Carpenter, Ph.D. Cascade Research Associates & Abbey Lane Laboratory microbe@peak.org cws1@axe.humboldt.edu wrote: : I've got a real headache of an isolate from Carex roots gathered from : alpine and subalpine meadows. If you might be able to offer suggestions : (it's not in Domsch and Gams) it has the following properties: : - clamp connections at every septa, extending about 2um from the hypha : - hypha about 1.6 - 4.5 um diameter : - cross connections frequent between hypha 30+ um apart. : - connidia found scattered about in small clusters, but no recognizeable : conideogenous cells; conidia abo9ut1.7 - 3.6 um diameter : - culture is powdery white. : - meadow soils were likely Histisols. : Any ideas? Thanks in advance! : Curt Seeliger cws1@axe.humboldt.edu : Humbled State University : Arcata, CA 95521 : The views expressed herein are "I can not live without books." : not only my views, but also the -- Thomas Jefferson : views of Humboldt State University : and the rest of the world as well, "I don't want to live in a country : so wise up bucko! that can't make it's own television : sets" : -- H. Ross Perot From owner-mycology@net.bio.net Tue Dec 05 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!in1.uu.net!newsfeed.ACO.net!news.univie.ac.at!news-admin@univie.ac.at From: Mayerhofer Thomas Newsgroups: bionet.mycology Subject: Cryphonectria parasitica Date: 6 Dec 1995 15:52:47 GMT Organization: Forschungszentrum Seibersdorf Lines: 22 Message-ID: <4a4e8f$k54@ftp.univie.ac.at> NNTP-Posting-Host: zdla12.arcs.ac.at Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) PLEASE tell me all you know about Cryphonectria parasitica !!!!! I am a Student here in Austria and I have started working on this Project recently so I need any Information I can get. Especially I need all the Stuff about the double stranded RNA Virus which makes it Hypovirulent and if there are Seqences of this RNA anywhere to get or even can send me some cDNA Clone ?!? :-) Thomas Mayerhofer Abteilung Agrarforschung und Biotechnologie Forschungszentrum Seibersdorf A-2444 Seibersdorf AUSTRIA Tel. : 02254-80-3525 Fax : 02254-80-2118 Email: Mayerhofer@zdfzs.arcs.ac.at From owner-mycology@net.bio.net Tue Dec 05 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!nntp.coast.net!torn!news.unb.ca!nimble.mta.ca!usenet From: Trevor W Lewis Newsgroups: bionet.mycology Subject: ARE POTATOES MYCORRHIZAL ???? Date: 6 Dec 1995 02:21:54 GMT Organization: Mount Allison University Lines: 7 Message-ID: <4a2uo2$bu2@nimble.mta.ca> NNTP-Posting-Host: 138.73.27.129 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.22 (Windows; I; 16bit) Anybody know the answer to this question? Please respond to the following e-mail address ASAP. Thanks, Trevor. twlws@mailserv.mta.ca From owner-mycology@net.bio.net Tue Dec 05 22:00:00 1995 Path: biosci!ESSEX.UCHSC.EDU!claudes From: claudes@ESSEX.UCHSC.EDU (Claude Selitrennikoff) Newsgroups: bionet.mycology Subject: expression plasmids for C. albicans Date: 6 Dec 1995 08:15:40 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <4a2uo2$bu2@nimble.mta.ca> NNTP-Posting-Host: net.bio.net Dear Group: I would like to express a protein in Candida albicans under the control of an inducible promoter (either metabolic or light or whatever). does anyone know of such a vector? Thanks for your help. Claude Selitrennikoff University of Colorado From owner-mycology@net.bio.net Wed Dec 06 22:00:00 1995 Path: biosci!MUWAYF.UNIMELB.EDU.AU!Hynes_Lab From: Hynes_Lab@MUWAYF.UNIMELB.EDU.AU (Hynes Lab) Newsgroups: bionet.mycology Subject: New WWW site for Aspergillu Date: 6 Dec 1995 16:29:53 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Subject: Time: 11:08 AM OFFICE MEMO New WWW site for Aspergillus Date: 12/7/95 Excuse the blatant self promotion but we have just opened up our WWW site covering Aspergillus nidulans and amdS regulation. We are also interested to hear from others who have Aspergillus related sites so that we can add links to these sites. The URL is http://hdlab.genetics.unimelb.edu.au Alex. From owner-mycology@net.bio.net Wed Dec 06 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!news.math.psu.edu!hudson.lm.com!asia.lm.com!not-for-mail From: Richard W. Kerrigan Newsgroups: bionet.mycology Subject: Re: Pacific NW field guides? Date: 7 Dec 1995 10:07:49 -0500 Organization: Telerama Public Access Internet, Pittsburgh, PA Lines: 28 Sender: sylvan@telerama.lm.com Message-ID: <4a7005$iqe@asia.lm.com> References: <49ngbo$q09@crl7.crl.com> NNTP-Posting-Host: asia.lm.com X-Newsreader: TIN [version 1.2 PL2] From distant memory: The Savory Wild Mushroom, by Stuntz and McKinney (?), has also been useful. Is it still in print? -- Rick Bob Galvan (kasplash@crl.com) wrote: : Dave Delacey wrote: : >I've been trying to ID the different fungi I've come across during the : >past two years using the Audubon's field guide, and I haven't had much : >success. Lack of effort, maybe? Is there a better guide for the area? : The field guide that all the west coast mycological societies (and : commercial pickers) use is _All That the Rain Promises, and More_, : by David Arora, about $20. : Then there is the more indepth _Mushrooms Demystified_ also by Arora. : about $30. : Most folks take "Rain" with them in their back pocket, and keep "DM" in : the car or at home, because it weighs about 5 lbs! -- Richard W. Kerrigan, Research Department, Sylvan Spawn Laboratory, Inc. 1163 Winfield Rd., Cabot, PA 16032 USA e-mail: rwk@sylvanres.com phone: 412-352-1521 fax: 412-352-4062 "Success has a thousand parents; failure is an orphan" From owner-mycology@net.bio.net Wed Dec 06 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!uwm.edu!msunews!netnews.upenn.edu!news.tju.edu!TJUVM.TJU.EDU!IEI036G From: IEI036G@TJUVM.TJU.EDU (Mark F. Miller) Newsgroups: bionet.mycology Subject: Oregon Mycological Society? Date: Thu, 07 Dec 95 00:57:09 EDT Organization: Thomas Jefferson University Lines: 10 Message-ID: <1746DD6B.IEI036G@TJUVM.TJU.EDU> NNTP-Posting-Host: tjuvm.tju.edu X-Newsreader: NNR/VM S_1.3.2 Greetings, Could some kind soul please post (or email) the contact address for the Oregon Mycological Society? Thank you very much. Regards, Mark Miller FAX: 215-464-3561 Mark.Miller@mail.tju.edu CIS: 70176, 1153 From owner-mycology@net.bio.net Wed Dec 06 22:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!newsfeed.internetmci.com!in1.uu.net!csn!nntp-xfer-2.csn.net!yuma!DRice.EHS.ColoState.EDU!drice From: drice@drice.ehs.colostate.edu (Doug Rice) Newsgroups: bionet.mycology Subject: Bioaerosol Web Site?? Date: Thu, 7 Dec 1995 16:05:42 GMT Organization: Colorado State University Lines: 10 Message-ID: NNTP-Posting-Host: drice.ehs.colostate.edu X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Good Morning: Is there a web site or a news group that concentrates on bioaerosols. I am specifically looking for an electronic tap into the pulse of Industrial Hygienists dealing with airborne fungi. THANKS! ________________________________________________________ Doug Rice: Laboratory Director; Environmental Quality Laboratory Microbiology of water, food, soil, and air. Colorado State University: Ft Collins, Colorado. e-mail: drice@vines.colostate.edu voice: (970) 491-6503 From owner-mycology@net.bio.net Thu Dec 07 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!psgrain!nntp.teleport.com!usenet From: "Ralph D. Arnold" Newsgroups: bionet.mycology Subject: Re: Oregon Mycological Society? Date: Thu, 7 Dec 95 19:45:14 PST Organization: Teleport - Portland's Public Access (503) 220-1016 Lines: 22 Message-ID: <81417.rarnold@teleport.com> Reply-To: NNTP-Posting-Host: ip-pdx08-45.teleport.com X-Minuet-Version: Minuet1.0_Beta_17A X-POPMail-Charset: English To: IEI036G@TJUVM.TJU.EDU I am the treasurer of the cultivation committee of O.M.S. and can supply you with details about both the society as a group and the cultivation committee in particular. Feel free to join us! Email me if you wish further details...and join us in 1996 for a GREAT mushrooming year! Ralph Arnold On Thu, 07 Dec 95 00:57:09 EDT, Mark F. Miller wrote: >Greetings, > >Could some kind soul please post (or email) the contact address for the >Oregon Mycological Society? > >Thank you very much. > > Regards, > Mark Miller FAX: 215-464-3561 > Mark.Miller@mail.tju.edu CIS: 70176, 1153 > From owner-mycology@net.bio.net Thu Dec 07 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!howland.reston.ans.net!news-e1a.megaweb.com!pop.gnn.com!Bugman3157 From: Bugman3157@gnn.com (Robert Domine) Newsgroups: bionet.mycology Subject: Aspergillus resistance to sanitizing agents Date: Thu, 07 Dec 1995 18:34:28 Organization: Megaweb Lines: 12 Message-ID: <4a7ua6$n19@news-e1a.megaweb.com> NNTP-Posting-Host: @www-20-208.gnn.com Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" X-GNN-NewsServer-Posting-Date: 7 Dec 1995 23:45:10 GMT X-Mailer: GNNmessenger 1.2 Can anyone recommend an effective sanitizing agent for ridding our pharmaceutical manufacturing facility of A. flavus and A. fumigatus? It must be non-corrosive to stainless steel and feasable for large scale application (spray, mop, fumigation). We have tried several actives with only moderate success. It appears that we have a particularly resistant (and prolific) strain contaminating our plant environment. Thanks From owner-mycology@net.bio.net Thu Dec 07 22:00:00 1995 Path: biosci!ns1.faseb.org!lamarck.sura.net!ra.nrl.navy.mil!news.math.psu.edu!hudson.lm.com!asia.lm.com!not-for-mail From: mspear@telerama.lm.com (mspear) Newsgroups: bionet.mycology Subject: Re: Infosift Anamorph Date: 8 Dec 1995 11:01:26 -0500 Organization: Telerama Public Access Internet, Pittsburgh, PA Lines: 27 Message-ID: <4a9ngm$b8a@asia.lm.com> References: <4a9n6n$b6d@asia.lm.com> NNTP-Posting-Host: asia.lm.com X-Newsreader: TIN [version 1.2 PL2] mspear (mspear@telerama.lm.com) wrote: > I've got the very useful database from Bryce Kendrick loaded onto my > little HP 200 palmtop and it works very well indeed. However, I > notice that the older database from the same source covering mushroom > genera does not show up as well on the screen. > Does anyone else use these programs? Do you know how to get Infosift > (the database engine) to display for monochrome screens? > Thanks in advance. > ---- Mark P.S. It is a pure pleasure to sit at a microscope and pull a good key out of your pocket. I have all kinds of programs on my desktop that are not much use in the lab unless I get another computer. The compactness and low resource overhead of these databases make them more useable than you would expect from a non-graphic, DOS program. Also the mushroom database goes into the field with me an my picking basket. Try to do that with a desktop. Thanks Bryce. ---- Mark From owner-mycology@net.bio.net Thu Dec 07 22:00:00 1995 Path: biosci!daresbury!bioftp.unibas.ch!news.vub.ac.be!usenet From: rlejeune@vnet3.vub.ac.be (Robert Lejeune) Newsgroups: bionet.mycology Subject: enzymatic resolution of racemic mixtures Date: 8 Dec 1995 10:12:22 GMT Organization: VUB Brussels Lines: 7 Message-ID: <4a9326$5b7@rc1.vub.ac.be> NNTP-Posting-Host: chispc2.vub.ac.be. Mime-Version: 1.0 X-Newsreader: WinVN 0.99.3 I am looking at more information (references) about the enzymatic resolution of a racemic mixture of D,L-tartaric acid (by a Penicillium fungi?). Ronnie Willaert From owner-mycology@net.bio.net Thu Dec 07 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!vixen.cso.uiuc.edu!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: dmall60379@aol.com (DMall60379) Newsgroups: bionet.mycology Subject: Re: Diverisity of fungi across continets Date: 7 Dec 1995 23:01:20 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 6 Sender: root@newsbf02.news.aol.com Message-ID: <4a8dag$dkp@newsbf02.news.aol.com> References: <49c574$c3l@newshost.rpms.ac.uk> Reply-To: dmall60379@aol.com (DMall60379) NNTP-Posting-Host: newsbf02.mail.aol.com Two of the species that you mention form ectomycorrhizae with forest trees and are probably not native to South Africa. It is likely that Amanita virosa, Boletus edulis and other common edible mushrooms in South Africa were introduced with tree partners such as pines, eucaypts, oaks, etc. Coprinus comatus does not form mycorrhizae and is probably widespread throughout the world. From owner-mycology@net.bio.net Thu Dec 07 22:00:00 1995 Path: biosci!ihnp4.ucsd.edu!usc!chi-news.cic.net!newsfeed.internetmci.com!in2.uu.net!fdn.fr!jussieu.fr!pasteur.fr!infobiogen.fr!bioftp.unibas.ch!news.vub.ac.be!ben.vub.ac.be!wmoens From: William Moens Newsgroups: bionet.mycology Subject: Preparation of Fungal DNA Date: Fri, 8 Dec 1995 14:16:41 +0000 Organization: Brussels Free Universities VUB/ULB Lines: 14 Message-ID: NNTP-Posting-Host: ben.vub.ac.be. Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII To whom it may concern: We published recently a paper (Journal of medical Mycology(1995) 5,133-139) validating a method for the preparation of fungal genomic DNA suitable for PCR and RAPD. This method has worked with 24 fungal genera covering about 200 species. Reprint can be obtained through Email or at W.Moens at Institute of Hygiene & Epidemiology Service of Biosafety & Biotechnology Wytsmanstreet, 14 B-1050 Brussels BELGIUM Fx: 322-6425292 Email wmoens@sbb.ihe.be From owner-mycology@net.bio.net Thu Dec 07 22:00:00 1995 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.hawaii.edu!ames!pendragon!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!newsfeed.internetmci.com!chi-news.cic.net!news.math.psu.edu!hudson.lm.com!asia.lm.com!not-for-mail From: mspear@telerama.lm.com (mspear) Newsgroups: bionet.mycology Subject: Re: Aspergillus resistance to sanitizing agents Date: 8 Dec 1995 10:50:14 -0500 Organization: Telerama Public Access Internet, Pittsburgh, PA Lines: 26 Message-ID: <4a9mrm$b4b@asia.lm.com> References: <4a7ua6$n19@news-e1a.megaweb.com> NNTP-Posting-Host: asia.lm.com X-Newsreader: TIN [version 1.2 PL2] Robert Domine (Bugman3157@gnn.com) wrote: > Can anyone recommend an effective sanitizing agent for ridding our > pharmaceutical manufacturing facility of A. flavus and A. > fumigatus? It must be non-corrosive to stainless steel and > feasable for large scale application (spray, mop, fumigation). > We have tried several actives with only moderate success. It > appears that we have a particularly resistant (and prolific) > strain contaminating our plant environment. > Thanks > We have found in similar situations that it is often not a true resistance to the biocide but a continual presence of an "encouraging" environment that is responsable for difficult outbreaks. If you are keeping a damp stale closed atmosphere in the plant you can nuke it with whatever you want and you won't get rid of the problem. Try culture plates ammended with the biocides to see if they are really resistant. Try a few large ceiling fans in poor air flow areas of the plant to see if that's the problem. ---- Mark From owner-mycology@net.bio.net Fri Dec 08 22:00:00 1995 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.hawaii.edu!ames!pendragon!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!newsfeed.internetmci.com!chi-news.cic.net!news.math.psu.edu!hudson.lm.com!asia.lm.com!not-for-mail From: mspear@telerama.lm.com (mspear) Newsgroups: bionet.mycology Subject: Infosift Anamorph Date: 8 Dec 1995 10:56:07 -0500 Organization: Telerama Public Access Internet, Pittsburgh, PA Lines: 12 Message-ID: <4a9n6n$b6d@asia.lm.com> NNTP-Posting-Host: asia.lm.com X-Newsreader: TIN [version 1.2 PL2] I've got the very useful database from Bryce Kendrick loaded onto my little HP 200 palmtop and it works very well indeed. However, I notice that the older database from the same source covering mushroom genera does not show up as well on the screen. Does anyone else use these programs? Do you know how to get Infosift (the database engine) to display for monochrome screens? Thanks in advance. ---- Mark From owner-mycology@net.bio.net Fri Dec 08 22:00:00 1995 Path: biosci!ns1.faseb.org!lamarck.sura.net!newsfeed.internetmci.com!dimensional.com!winternet.com!guitar.sound.net!news From: dwimmer@smartnet.net Newsgroups: bionet.mycology Subject: Re: Aspergillus resistance to sanitizing agents Date: Fri, 08 Dec 1995 22:41:26 -0500 Organization: Sound Advice Limited's Internet Access for KC Lines: 21 Message-ID: <30C90566.664B@smartnet.net> References: <4a7ua6$n19@news-e1a.megaweb.com> NNTP-Posting-Host: dialup14.smartnet.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b2 (Macintosh; I; PPC) Robert Domine wrote: > > Can anyone recommend an effective sanitizing agent for ridding our > pharmaceutical manufacturing facility of A. flavus and A. > fumigatus? It must be non-corrosive to stainless steel and > feasable for large scale application (spray, mop, fumigation). > We have tried several actives with only moderate success. It > appears that we have a particularly resistant (and prolific) > strain contaminating our plant environment. > > Thanks As in the previous reply, you need to look at your facility design first. You should expect to see a lot of Aspergillus and Penicillum in your unclassified areas, especially if your HVAC systems don't have good prefilters. In your classified areas, you shouldn't be seeing the stuff, especially as the classification increases. Without knowing more about your situation, it is hard to help- is this localized in one area, what is the area classification, equipment/processes in the area, etc.? I know of a situation where there was HEPA-filtered air supplied to a lab area which was quite some way downstream of the filter and the diffuser had no filter on it. Someone had found that quite a bit of dust had built up on the louvres, which was causing molds to blow into the area. From owner-mycology@net.bio.net Fri Dec 08 22:00:00 1995 Path: biosci!CS.Arizona.EDU!news.Arizona.EDU!hamblin.math.byu.edu!acs2.byu.edu!news.cuny.edu!caen!reeve.research.aa.wl.com!decwrl!lll-winken.llnl.gov!fnnews.fnal.gov!nntp-server.caltech.edu!alumni.caltech.edu!bwoods From: bwoods@alumni.caltech.edu (Bradley B. Woods) Newsgroups: bionet.mycology Subject: Re: Any Mycology groups in SoCal? Date: 9 Dec 1995 01:05:05 GMT Organization: California Institute of Technology, Pasadena Lines: 12 Message-ID: <4aanc1$bul@gap.cco.caltech.edu> References: <49lp88$g90@gap.cco.caltech.edu> <49sljr$3j3@news1.deltanet.com> NNTP-Posting-Host: alumni.caltech.edu X-Newsreader: TIN [version 1.2 PL2] bwoods@alumni.caltech.edu (Bradley B. Woods) wrote: >Hi, I realize it ain't the best place to go looking for mushrooms, but it >is where I live, so are there any amateur mycology groups in southern >California? >B I want to thank everyone who replied in this thread or by e-mail. I definitely know where to go now. B From owner-mycology@net.bio.net Sat Dec 09 22:00:00 1995 Path: biosci!ihnp4.ucsd.edu!agate!news.Stanford.EDU!nntp-hub2.barrnet.net!news1.digital.com!uunet!in2.uu.net!newsfeed.internetmci.com!news.fyionline.com!op.net!en.com!news.netnet.net!kryten.awinc.com!news From: bigamj@cadvision.com (Jay Bigam) Newsgroups: bionet.mycology Subject: Mountain Mushrooms - Regular Posting Date: 7 Dec 1995 17:18:37 GMT Organization: A & W Internet Inc. Lines: 40 Message-ID: <4a77ld$6kt@kryten.awinc.com> NNTP-Posting-Host: pmeb18.access.awinc.com Mime-Version: 1.0 X-Newsreader: WinVN 0.99.3 Mountain Mushrooms - Regular Posting Mountain Mushrooms proudly announces it's Internet Wild Mushroom home page and order system located at: http://www.catalog.com/monafood/ Mountain Mushrooms picks it's wild mushrooms in the pristine mountain ranges and forests of western Canada. Clean air, soil and good weather provide prime mushroom growing conditions and the highest quality product. Hand picked fresh mushrooms are delivered immediately to mobile dehydrators to be dried and packaged to preserve optimum flavour and quality. With a moisture content of 5-8%, all our dehydrated mushrooms are completely free of additives and preservatives and are packed in air tight containers to preserve freshness. They can be stored for up to a year with no loss of flavour or quality. Mountain Mushrooms are an excellent source of many nutrients. Long known for curative and restorative properties, these mushrooms are a healthy and delicious addition to any diet. Order Direct On The Net Distributors Welcome! For More Information Contact Mountain Mushrooms Suite 2830, Manulife Place 10180-101 Street Edmonton, AB, Canada T5J 3S4 (403) 228-1327 (403) 244-5970 Fax( 403) 421-1320 Email bigamj@cadvision.com From owner-mycology@net.bio.net Sun Dec 10 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!tank.news.pipex.net!pipex!dish.news.pipex.net!pipex!news.esoterica.pt!usenet From: jcb1@mail.esoterica.pt (jcb1) Newsgroups: bionet.mycology Subject: Test - Ignore Date: 10 Dec 1995 17:00:05 GMT Organization: esoterica.pt Lines: 2 Message-ID: <4af3ml$qqd@orthanc.esoterica.com> Reply-To: jcb1@esoterica.pt NNTP-Posting-Host: lisslip5.esoterica.pt Mime-Version: 1.0 X-Newsreader: WinVN 0.93.10 fg From owner-mycology@net.bio.net Sun Dec 10 22:00:00 1995 Path: biosci!agate!news.ucdavis.edu!library.ucla.edu!newsfeed.internetmci.com!howland.reston.ans.net!EU.net!peer-news.britain.eu.net!uknet!str-ccsun!usenet From: David Currie Newsgroups: bionet.mycology Subject: field guide to the carribean Date: 11 Dec 1995 15:56:39 GMT Organization: University of Strathclyde Computer Centre Lines: 8 Message-ID: <4ahkbn$831@rockall.cc.strath.ac.uk> NNTP-Posting-Host: d-currie.biosci.strath.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 32bit) To: newsgroup:bionet.mycology Hi there, I am soon to visit the island of St Lucia in the South eastern Carribean. Does anyone know of a field guide for basidiomycetes for this area?> > -------------------------------------------------------------------- > From owner-mycology@net.bio.net Sun Dec 10 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!gatech2!EU.net!peer-news.britain.eu.net!uknet!str-ccsun!usenet From: David Currie Newsgroups: bionet.mycology Subject: field guide to the carribean Date: 11 Dec 1995 15:41:08 GMT Organization: University of Strathclyde Computer Centre Lines: 6 Message-ID: <4ahjek$6tf@rockall.cc.strath.ac.uk> NNTP-Posting-Host: d-currie.biosci.strath.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 32bit) To: bionet.mycology Hi there, I am soon to visit the island of St Lucia in the South eastern Carribean. Does anyone know of a field guide for basidiomycetes for this area? From owner-mycology@net.bio.net Sun Dec 10 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!EU.net!sun4nl!Utrecht.NL.net!news.via.nl!usenet From: mushvg@ns.via.nl Newsgroups: bionet.mycology Subject: transfer failure Date: Mon, 11 Dec 95 11:19:03 PDT Organization: Venlo Internet Access Lines: 8 Message-ID: <4ah0s1$l0t@news.via.nl> NNTP-Posting-Host: modems9.via.nl Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Newsreader: NEWTNews & Chameleon -- TCP/IP for MS Windows from NetManage Appr. a week ago I posted a message about a postdoc position available in our Experimental Station. One of the responders was: cibbb4i@PINAR1.csic.es. The response could not be delivered because of transfer failure or maximum time expired. I tried to contact the same adress; again no transfer because of wrong adress. Sorry to use this forum to solve the problem. Please contact again by e-mail or by fax 31 77464 7575. Thank you. From owner-mycology@net.bio.net Sun Dec 10 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!EU.net!sun4nl!Utrecht.NL.net!news.via.nl!usenet From: mushvg@ns.via.nl Newsgroups: bionet.mycology Subject: Re: Preparation of Fungal DNA Date: Mon, 11 Dec 95 09:36:25 PDT Organization: Venlo Internet Access Lines: 40 Message-ID: <4agqj5$jqp@news.via.nl> References: NNTP-Posting-Host: modems0.via.nl Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII To: wmoens@sbb.ihe.be X-Newsreader: NEWTNews & Chameleon -- TCP/IP for MS Windows from NetManage In article , writes: > Path: news.via.nl!Utrecht.NL.net!sun4nl!EU.net!howland.reston.ans.net!nntp.coast. net!lll-winken.llnl.gov!uwm.edu!newsfeed.internetmci.com!in2.uu.net!fdn.fr! jussieu.fr!pasteur.fr!infobiogen.fr!bioftp.unibas.ch!news.vub.ac.be!ben.vub ac.be!wmoens > From: William Moens > Newsgroups: bionet.mycology > Subject: Preparation of Fungal DNA > Date: Fri, 8 Dec 1995 14:16:41 +0000 > Organization: Brussels Free Universities VUB/ULB > Lines: 14 > Message-ID: > NNTP-Posting-Host: ben.vub.ac.be. > Mime-Version: 1.0 > Content-Type: TEXT/PLAIN; charset=US-ASCII > > To whom it may concern: > > We published recently a paper (Journal of medical Mycology(1995) > 5,133-139) validating a method for the preparation of > fungal genomic DNA suitable for PCR and RAPD. This method has worked with > 24 fungal genera covering about 200 species. > Reprint can be obtained through Email or at > > W.Moens at Institute of Hygiene & Epidemiology > Service of Biosafety & Biotechnology > Wytsmanstreet, 14 > B-1050 Brussels BELGIUM > Fx: 322-6425292 > Email wmoens@sbb.ihe.be I would like to receive reprint of the paper mentioned. Thanks. L.J.L.D. Van Griensven Mushroom Exptl Stat POB 6042 5960 AA Horst The Netherlands From owner-mycology@net.bio.net Sun Dec 10 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!usenet.eel.ufl.edu!psgrain!nntp.teleport.com!nntp.teleport.com!not-for-mail From: larryc@teleport.com (Larry Caldwell) Newsgroups: bionet.mycology Subject: Re: Aspergillus resistance to sanitizing agents Date: 10 Dec 1995 11:05:04 -0800 Organization: Teleport - Portland's Public Access (503) 220-1016 Lines: 34 Message-ID: References: <4a7ua6$n19@news-e1a.megaweb.com> NNTP-Posting-Host: julie.teleport.com In article <4a7ua6$n19@news-e1a.megaweb.com>, Bugman3157@gnn.com (Robert Domine) wrote: > Can anyone recommend an effective sanitizing agent for ridding our > pharmaceutical manufacturing facility of A. flavus and A. > fumigatus? It must be non-corrosive to stainless steel and > feasable for large scale application (spray, mop, fumigation). > We have tried several actives with only moderate success. It > appears that we have a particularly resistant (and prolific) > strain contaminating our plant environment. Check with local hazardous materials contractors. Specialists in asbestos, heavy metal and solvent abatement often have resources that can help you out. There are 2-part activation disinfectants that will do what you want. Bio-rid is one trade name I remember. I've tackled this problem at medical school research labs, and found the primary source of contamination was either ductwork or porous surfaces like suspended ceiling tile. Fiber duct liners, originally installed for sound deadening, are often a major source of contamination. We developed a number of strategies for dealing with the problem. Ductwork can be brushed and vacuumed, followed by application of a disinfectant and sealant. Ceiling tile can be vacuumed and sealed. An impermeable barrier can be installed over surfaces that can't be sealed. The real trick is accomplishing the work without shutting down the facility, since the work naturally will stir up greatly increased levels of contamination. Good luck. -- Larry Caldwell Myrtle Creek, Oregon From owner-mycology@net.bio.net Sun Dec 10 22:00:00 1995 Newsgroups: bionet.mycology Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!torn!nott!cunews!freenet.carleton.ca!FreeNet.Carleton.CA!by207 From: by207@FreeNet.Carleton.CA (Satoko Hayashi) Subject: FUSCOPORIA OBLIQUA Message-ID: Sender: by207@freenet.carleton.ca (Satoko Hayashi) Reply-To: by207@FreeNet.Carleton.CA (Satoko Hayashi) Organization: The National Capital FreeNet Date: Mon, 11 Dec 1995 21:39:59 GMT Lines: 17 I am looking for somebody who is involved in a research on Fuscoporia obliqua, a kind of mushrooms grown on white birch. Professor Ueno of Science University of Tokyo is seeking a collaborative project on AIDS treatment. I would appreciate any kind of information on this subject. Thank you. Satoko Hayashi by207@FreeNet.Carleton.CA From owner-mycology@net.bio.net Sun Dec 10 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!nntp.coast.net!swidir.switch.ch!in2p3.fr!univ-lyon1.fr!ws41.cnusc.fr!ciril.fr!univ-lille1.fr!usenet From: Regis.Courtecuisse@phare.univ-lille2.fr (Regis.Courtecuisse) Newsgroups: bionet.mycology Subject: Re: field guide to the carribean Date: 11 Dec 1995 17:54:36 GMT Organization: Your Organization Lines: 38 Message-ID: <4ahr8s$h8m@netserver.univ-lille1.fr> References: <4ahjek$6tf@rockall.cc.strath.ac.uk> NNTP-Posting-Host: bolet.univ-lille2.fr Mime-Version: 1.0 X-Newsreader: WinVN 0.93.10 In article <4ahjek$6tf@rockall.cc.strath.ac.uk>, D.A.Currie@strath.ac.uk says... > >Hi there, >I am soon to visit the island of St Lucia in the South eastern >Carribean. >Does anyone know of a field guide for basidiomycetes for this >area? > Hi ! The only (recent and rather complete) book on Basidiomycetes concerning the Caribbean area is the "Agaric Flora of the Lesser Antilles" by David N. Pegler (Kew Bull.Add.Ser.IX:668 pp. + 28 pl. -published 1983) It can't be considered as a field guide because of its weight...! I am afraid that is the single and only thing I can recommend. By the way, let me introduce myself as a specialist in taxonomy of neotropical agarics (gilled fungi). I work especially on the guianese area (Northern South America, French Guiana, Guyana and Surinam) and I am involved in the "Flora of the Guianas" serial publication. I am now starting a further program on the Caribbean area. Much remains to be done, even after the good work by Pegler cited above. I was last year in Guadeloupe and Saint Martin and I'll visit several islands next February. Whenever you would aggree with the idea of providing material for further examination and identification, I would be very pleased to send you a "protocol for the collection and preparation of mycological paterial" I prepared and published for naturalist of different addinities -not especially mycologists- so that they could collect valuable material from any tropical -or other- area. Please let me know if you would accept to collaborate this way. Looking forward to earing from you soon. Best regards and wishes. Regis. From owner-mycology@net.bio.net Mon Dec 11 22:00:00 1995 Path: biosci!newshost.lanl.gov!usenet From: 74627.3370@compuserve.com (Ted Stampfer) Newsgroups: bionet.mycology Subject: South American Field Guide Date: 12 Dec 1995 04:21:46 GMT Organization: Los Alamos National Laboratory Lines: 7 Sender: -Not-Authenticated-[4327] Message-ID: <4aj00q$29h@newshost.lanl.gov> NNTP-Posting-Host: transitory7.lanl.gov X-Posted-From: InterNews 1.0.6@transitory7.lanl.gov Xdisclaimer: No attempt was made to authenticate the sender's name. I would appreciate any suggestions for a good field guide for southern South America, preferably in English? Thank you. Ted Stampfer From owner-mycology@net.bio.net Mon Dec 11 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!nntp.coast.net!oleane!jussieu.fr!univ-lille1.fr!usenet From: Regis.Courtecuisse@phare.univ-lille2.fr (Regis.Courtecuisse) Newsgroups: bionet.mycology Subject: Re: South American Field Guide Date: 12 Dec 1995 11:00:34 GMT Organization: Your Organization Lines: 25 Message-ID: <4ajnci$vn@netserver.univ-lille1.fr> References: <4aj00q$29h@newshost.lanl.gov> NNTP-Posting-Host: bolet.univ-lille2.fr Mime-Version: 1.0 X-Newsreader: WinVN 0.93.10 In article <4aj00q$29h@newshost.lanl.gov>, 74627.3370@compuserve.com says... > >I would appreciate any suggestions for a good field guide for southern >South America, preferably in English? > >Thank you. > >Ted Stampfer > I know no true field-guide (if understood as a bok with nice colour photographs or illustrations in a pocket format...) for this area. May I suggest that you try the following (very good) E.Horak -1979- Flora Criptogamica de tierra del fuego/ Tomo XI(fasc.6), Fungi, Basidiomycetes. Agaricales y Gasteromycetes secotioides. 524 pp. (in spanish!) (an address on the book is "Funfacion para la Educacion, la Cicencia y la Cultura. Moreno 431, Buenos Aires, Argentina"). I don't know if it is still available... Other books exist. Is this too southern ? Regards and hope this helps ! Régis From owner-mycology@net.bio.net Mon Dec 11 22:00:00 1995 Path: biosci!botany.uq.edu.au!J.Manners From: J.Manners@botany.uq.edu.au ("MANNERS, DR. John M.") Newsgroups: bionet.mycology Subject: phleomycin transformed strains Date: 11 Dec 1995 20:52:23 -0800 Organization: Dept of Botany, Univ of Qld Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hi Everyone, We are interested in the 'horizontal transfer' of specific chromosomes between normally genetically incompatible fungal biotypes and species. We aer using specific chromosomes tagged with selectable marker genes (usually hygR) in donor strains and recipient strains tagged with phleomycin resistance. So far our work has focused on Colletotrichum gloeosporioides but we are now interested in trying other species as recipients. Therefore I am interested in accessing some strains of ascomycete/deuteromycete fungi which are transformed for resistance to phleomycin. The level of resistance should be higher than 10 micrograms per ml. If anyone can help I would be very grateful. Please reply directly to my Email address. thanks a lot John Manners John Manners The CRC for Tropical Plant Pathology Level 5, John Hines Building The University of Queensland 4072, Australia Telephone: 61-7-38703773 Facsimile: 61-7-33654771 Email: j.manners@botany.uq.edu.au From owner-mycology@net.bio.net Mon Dec 11 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!usc!news.cerf.net!newsserver.sdsc.edu!news.tc.cornell.edu!newsstand.cit.cornell.edu!NewsWatcher!user From: kh11@cornell.edu (Kathie T. Hodge) Newsgroups: bionet.mycology Subject: Mycology on the WWW Date: 11 Dec 1995 18:29:11 GMT Organization: Cornell University Lines: 17 Sender: kh11@cornell.edu (Verified) Message-ID: NNTP-Posting-Host: 128 Those of you seeking fungal information on the internet might be interested in the recently updated web pages I maintain for the WWW Virtual Library: http://muse.bio.cornell.edu/taxonomy/fungi.html The pages are a compilation of all the mycology-related internet sites that I can find. If your favorite site is missing, please let me know! Online information on all manner of other subjects can be found through the other branches of the WWW VL. Click on the Library icon at the top of my pages to see the subject index. best wishes, Kathie Hodge kh11@cornell.edu maintainer, WWW VL: Mycology From owner-mycology@net.bio.net Tue Dec 12 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!demon!mail2news.demon.co.uk!scottp.demon.co.uk From: Richard Scott Newsgroups: bionet.mycology Subject: EPIDEMIOLOGICAL SURVEY IN RURAL ASIA Date: Wed, 13 Dec 1995 00:19:02 GMT Organization: Myorganisation Lines: 26 Message-ID: <317456335wnr@scottp.demon.co.uk> Reply-To: Richard@scottp.demon.co.uk X-NNTP-Posting-Host: scottp.demon.co.uk X-Broken-Date: Wednesday, Dec 13, 1995 00.19.02 X-Newsreader: Newswin Alpha 0.7 X-SMTP-Posting-Host: scottp.demon.co.uk [Wed, 13 Dec 95 0:25:58 GMT] X-SMTP-Posting-Host: post.demon.co.uk [Wed, 13 Dec 95 0:26:22 GMT] I am a 2nd year pre-clinical medical student at Cambridge University (UK); A friend (another medic from Cambridge), are travelling in Tibet for 3 months next summer (July until October) and are interested in carrying out a medically related survey. We will be travelling in 'urban' areas, but probably of more use would be the contact we will also have with very rural (including nomadic) communities. We anticipate that we will trek in such rural (high altitude) areas for at least a month of the 3. We would be more than happy to carry out a survey as part of someone elses research. Possibilities might include surveys into: (1) immunization programs (2) disease epidemiology (3) genetic disease prevalence (4) water quality (especially water borne diseases) (5) nutrition however, we are open to your suggestions. Thank you very much, Richard Scott e-mail: richard@scottp.demon.co.uk From owner-mycology@net.bio.net Tue Dec 12 22:00:00 1995 Newsgroups: bionet.mycology Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sisna.com!NewsWatcher!user From: squash@Flairnet.com (Mike Clark) Subject: ?Health effects of Stachybotrys chartarum? Message-ID: Date: Tue, 12 Dec 1995 20:21:14 -0500 Lines: 7 I am a high school biology teacher seeking any information on the possible health effects of S. chartarum. It has been positively identified in an older middle school where at least two teachers have suffered disabling chronic illnesses. Three species of Aspergillus were also identified at the same location. I would appreciate any info or suggestions as to where else to look. My e-mail address: Mike_Clark@auburn.cnyric.org From owner-mycology@net.bio.net Tue Dec 12 22:00:00 1995 Path: biosci!MANI.CBS.UMN.EDU!npv From: npv@MANI.CBS.UMN.EDU (Nora Plesofsky-Vig) Newsgroups: bionet.mycology Subject: nitrotetrazolium blue assays Date: 12 Dec 1995 17:10:12 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <9512130106.AA00941@mani.cbs.umn.edu> Reply-To: nora@biosci.cbs.umn.edu NNTP-Posting-Host: net.bio.net We are interested in trying the nitrotetrazolium blue assay with Neurospora to test for respiratory activity under different conditions. I have a reference to this assay in regard to succinic dehydrogenase activity, as well as a reference to a general respiratory assay that uses 2,3,5-triphenyl-2H-tetrazolium chloride. If anyone has information or a reference on the use of the nitrotetrazolium blue to measure respiration, including the components used in the overlay, I would appreciate hearing about it. Thanks alot. Nora Plesofsky-Vig nora@biosci.cbs.umn.edu From owner-mycology@net.bio.net Tue Dec 12 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!nntp.coast.net!swidir.switch.ch!in2p3.fr!univ-lyon1.fr!jussieu.fr!univ-lille1.fr!usenet From: Regis.Courtecuisse@phare.univ-lille2.fr (Regis.Courtecuisse) Newsgroups: bionet.mycology Subject: Re: Gymnopilus spectabilis -- psylocibin/psylocin? Date: 13 Dec 1995 14:46:07 GMT Organization: Your Organization Lines: 32 Message-ID: <4amovf$7b8@netserver.univ-lille1.fr> References: NNTP-Posting-Host: bolet.univ-lille2.fr Mime-Version: 1.0 X-Newsreader: WinVN 0.93.10 In article , willikh0@seraph1.sewanee.edu says... > >hi there, > > can anyone out there give me an accurate estimate of the average ratio >of psylocibin/psylocin content between P. cubensis and Gymnopilus >spectabilis? I have heard that the latter contains enough ps. to be >psychoactive... is this true? > your humble servant, > C. Lamp Connection > Hi ! Gymnopilus spectabilis is said to be psychoactive but it seems that it works only in some areas of the world. European specimens tested proved to be devoid of any psilocybin and related tryptamines (Stijve & Kuyper, 1988, Persoonia 13(4):464) Concerning North American specimens, it depends of the collecting area. The same authors (see above) also studied an US collection and found it to be devoid of the mentioned compounds. Arora (1986, 2nd.Ed.) in Mushrooms demystified tells something about that (p.410). I hope this is only scientific curiosity... Gymnopilus is too bitter anyway ! And just have a trip through the observation and study of fungi. It is really fun ! Régis From owner-mycology@net.bio.net Tue Dec 12 22:00:00 1995 Path: biosci!utmb.edu!lester.pasarell From: lester.pasarell@utmb.edu Newsgroups: bionet.mycology Subject: Re: ?Health effects of Stachybotrys chartarum? Date: 13 Dec 1995 06:49:08 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 49 Sender: daemon@net.bio.net Distribution: world Message-ID: References: Reply-To: lester.pasarell@utmb.edu NNTP-Posting-Host: net.bio.net On Tue, 12 Dec 1995, Mike Clark wrote: > I am a high school biology teacher seeking any information on the possible > health effects of S. chartarum. It has been positively identified in an > older middle school where at least two teachers have suffered disabling > chronic illnesses. Three species of Aspergillus were also identified at > the same location. > I would appreciate any info or suggestions as to where else to look. > My e-mail address: Mike_Clark@auburn.cnyric.org > > > As with anything here in the bionet groups related to patient health ...any clinical symptom experienced by you or any other person should be first consulted with a physician...but the literature shows that this organism could be quite damaging to crops, building materials humans and animals due to its toxins , in the last two..humans and animals ingestion or inhalation can cause: dermatitis, burning sensation in the mouth, mucosal irration of nose and other respiratory sites and severe pathological changes such as low white blood cell and platelets counts in the circulating blood. reference: Microfungi by Susan Gravesen, Jens C. Frisvad, Robert A. Samson and Munksgaard. 1994. High Tech Prepress A/S, Copenhagen. Printed in Denmark by Special-Trykkeriet Viborg a/s Isbn 87-16-11436-1 I am no expert in this field... but information about this fungus could also be obtained doing a Medline search or by reviewing one of the other excellent books available for Medical Mycology or Industrial Mycology published in the past years Good luck lester **************************************************************************** Lester Pasarell-Luiggi Lester.Pasarell@utmb.edu Medical Mycology Research laboratory LPASAREL@beach.utmb.edu University of Texas Medical Branch Galveston, Texas http://fungus.utmb.edu furuki o tazune atarashiki o shiru Study the old to understand the new --- Japanese Proberb----- ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ From owner-mycology@net.bio.net Tue Dec 12 22:00:00 1995 Path: biosci!lhc.nlm.nih.gov!darwin.sura.net!cherub.sewanee.edu!NewsWatcher!user From: willikh0@seraph1.sewanee.edu (coon dog) Newsgroups: bionet.mycology Subject: Gymnopilus spectabilis -- psylocibin/psylocin? Date: 13 Dec 1995 09:09:28 GMT Organization: it's a secret Lines: 25 Message-ID: NNTP-Posting-Host: 152.97.75.169 X-Newsreader: Value-Added NewsWatcher 2.0b24.0+ hi there, can anyone out there give me an accurate estimate of the average ratio of psylocibin/psylocin content between P. cubensis and Gymnopilus spectabilis? I have heard that the latter contains enough ps. to be psychoactive... is this true? your humble servant, C. Lamp Connection ************************************************************* *...........Oh out near Stonehenge, I lived alone...........* ************************************************************* * * * .-'~~~-. willikh0@seraph1.sewanee.edu * * .'o oOOOo`. box 1447 * * :~~~-.oOo o`. 735 university ave * * `. \ ~-. oOOo. sewanee, tn 37383-1000 * * `.; / ~. OO: * * .' ;-- `.o.' * * ,' ; ~~--'~ * * ; ; * *_\\;_\\//_ * ************************************************************* From owner-mycology@net.bio.net Tue Dec 12 22:00:00 1995 Path: biosci!daresbury!not-for-mail From: lus@taxus.oesa.ufz.de (Nobert Luschka) Newsgroups: bionet.mycology Subject: Re: South American Field Guide Date: 12 Dec 1995 17:54:47 -0000 Lines: 27 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4akfl7$5vm@mserv1.dl.ac.uk> X-Sender: lus@taxus.oesa.ufz.de Original-To: mycology@dl.ac.uk >In article <4aj00q$29h@newshost.lanl.gov>, 74627.3370@compuserve.com >says... >> >>I would appreciate any suggestions for a good field guide for southern >>South America, preferably in English? >> >>Thank you. >> >>Ted Stampfer >> > One possibility of a field-key at least for Agaricales is "J. Raithelhuber: Flora mycologia Argentina. Hongos III." Metrodiana 1991. It is in spanish with an english key (156 p.) Norbert Luschka Dr. Nobert Luschka Sektion Biozoenoseforschung (Dep. of Community Ecology) Umweltforschungszentrum Leipzig-Halle GmbH (Centre for Environmental Research Leipzig-Halle Ltd.) Hallesche Strasse 44 D-06246 Bad Lauchstaedt, Germany Tel.:(+34635)73-291 Fax: (+34635)73-237 E-Mail: lus@oesa.ufz.de From owner-mycology@net.bio.net Tue Dec 12 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.kei.com!nntp.et.byu.edu!news.byu.edu!hamblin.math.byu.edu!news.Arizona.EDU!news.Cerritos.edu!newshub.csu.net!charnel.ecst.csuchico.edu!waldorf.csc.calpoly.edu!polyslo.csc.calpoly.edu!not-for-mail From: dgross@polyslo.csc.calpoly.edu (David Gross) Newsgroups: bionet.mycology,alt.drugs.psychedelics,rec.drugs.psychedelic Subject: Hypertext magic mushroom grower's guides & field guides available Date: 12 Dec 1995 16:26:03 -0800 Organization: Ventura Ventura Space People Lines: 12 Message-ID: <4al6ir$pmi@polyslo.csc.calpoly.edu> Reply-To: dgross@polyslo.CalPoly.EDU (Dave Gross) NNTP-Posting-User: dgross@polyslo.csc.calpoly.edu Xref: biosci bionet.mycology:3245 rec.drugs.psychedelic:6157 Hypertext magic mushroom grower's guides and field guides are available free-of-charge at the Sputnik Drug Information Zone: http://www.nepenthes.xo.com/Plants/shrooms/index.html This is quite probably the best resource on the net for the magic mushroom grower and/or hunter. -- ***** INTERNET: dgross@polyslo.CalPoly.EDU ***** dgross@morlock.punk.net ****** "Symbolic representation of quantitative entities is doomed to its rightful place of minor importance in a world where flowers and beautiful women abound." -- Albert Einstein From owner-mycology@net.bio.net Tue Dec 12 22:00:00 1995 Path: biosci!PLANTPATH.WISC.EDU!SAL From: SAL@PLANTPATH.WISC.EDU ("Sally Ann Leong") Newsgroups: bionet.mycology Subject: unsubscribe Date: 13 Dec 1995 09:48:32 -0800 Organization: University of Wisconsin Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <156EDD1663@charon.plantpath.wisc.edu> Reply-To: sal@plantpath.wisc.edu NNTP-Posting-Host: net.bio.net Sal@plantpath.wisc.edu From owner-mycology@net.bio.net Tue Dec 12 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!swrinde!cssun.mathcs.emory.edu!hobbes.cc.uga.edu!UGA.CC.UGA.EDU!BLACKMJ From: BLACKMJ@UGA.CC.UGA.EDU (Michael J. Black) Newsgroups: bionet.mycology Subject: Re: ?Health effects of Stachybotrys chartarum? Date: Wed, 13 Dec 95 16:52:44 EST Organization: University of Georgia Lines: 44 Message-ID: <17473ED5ES86.BLACKMJ@UGA.CC.UGA.EDU> References: NNTP-Posting-Host: uga.cc.uga.edu >On Tue, 12 Dec 1995, Mike Clark wrote: > >> I am a high school biology teacher seeking any information on the possible >> health effects of S. chartarum. It has been positively identified in an >> older middle school where at least two teachers have suffered disabling >> chronic illnesses. Three species of Aspergillus were also identified at >> the same location. Stachybotrys spp. occur uncommonly on cellulosic substrates. This means it can potentially grow on ceiling tiles, particle board, paper wrapping, cellulosic insullation, and storage materials such as boxes. It has a reputation of being a human health hazard since one or more species are capable of producing mycotoxins. This reputation may not necessarily be warranted. It has been pretty well documented, primarily from the Balkans, to cause disease in farm animals that ingest or sleep on hay infested with S. atra. A few human cases have been documented, but are associated only with contact of moldy straw, I believe. To the best of my knowledge no human or veterinary case has been linked to inhalation of this organism's conidia, or spores. It has been shown that the mycotoxin can occur in the conidia and presumably this means that the mycotoxin can be inhaled. It has also been shown that S. atra can produce the mycotoxin on subtrates as simple as MCE filter paper, suggesting that common building materials would be suitable substrates for mycotoxin production. Sure, Stachybotrys has the potential to be a problematic organism in indoor settings, but so little evidence has been collected to support such assertions that fears of this critter are probably unwarranted. Aspergillus would be an unlikely candidate to cause multiple illnesses in an indoor setting such as a school building and is probably not the source of your problem, but this is just my opinion. Many fungi produce volatile compounds which could potentially cause problems if ventilation within your building is poor. I personally would recommend that you insist that the ventilation system be properly inspected to insure an adequate intake of fresh air. Also, inspect your building for signs of water damage. Repair damaged areas and leaks, and remove water damaged materials. Let me know if you need a list of the better references on S. atra. Michael Black From owner-mycology@net.bio.net Tue Dec 12 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!vixen.cso.uiuc.edu!milo.mcs.anl.gov!mac108.tcg.anl.gov!user From: burleigh@tcg.anl.gov (darin) Newsgroups: bionet.mycology Subject: Re: Gymnopilus spectabilis -- psylocibin/psylocin? Followup-To: bionet.mycology Date: 13 Dec 1995 19:25:45 GMT Organization: Argonne National Laboratory Lines: 21 Message-ID: References: NNTP-Posting-Host: mac108.tcg.anl.gov In article , willikh0@seraph1.sewanee.edu (coon dog) wrote: > hi there, > > can anyone out there give me an accurate estimate of the average ratio > of psylocibin/psylocin content between P. cubensis and Gymnopilus > spectabilis? I have heard that the latter contains enough ps. to be > psychoactive... is this true? > > your humble servant, > > C. Lamp Connection > Illinois specimens appear to be active. -- darin burleigh@tcg.anl.gov "cogito cogito ergo cogito sum" - ambrose bierce From owner-mycology@net.bio.net Wed Dec 13 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!newsfeed.ACO.net!sbg.ac.at!DPFL18!zorer From: zorer@sbg.ac.at Newsgroups: bionet.mycology Subject: Second (Final) Circular of the THIRD IAL3 Date: Thu, 14 Dec 1995 10:24:35 GMT Organization: Computing Services, University of Salzburg Lines: 11 Message-ID: NNTP-Posting-Host: dpfl18.pfl.sbg.ac.at X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] The Second (Final) Circular IAL3 The third IAL SYMPOSIUM PROGRESS AND PROBLEMS IN LICHENOLOGY IN THE NINETIES SALZBURG, AUSTRIA 1 - 7 September 1996 is avaible under: http://www.sbg.ac.at/pfl/projects/lichen/index.htm From owner-mycology@net.bio.net Thu Dec 14 22:00:00 1995 Path: biosci!PLANTPATH.WISC.EDU!SAL From: SAL@PLANTPATH.WISC.EDU ("Sally Ann Leong") Newsgroups: bionet.mycology Subject: unsubscribe Date: 15 Dec 1995 10:49:14 -0800 Organization: University of Wisconsin Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <4674874793@charon.plantpath.wisc.edu> Reply-To: sal@plantpath.wisc.edu NNTP-Posting-Host: net.bio.net sal@plantpath.wisc.edu From owner-mycology@net.bio.net Thu Dec 14 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!nntp.coast.net!news00.sunet.se!sunic!news99.sunet.se!news.uni-c.dk!ut.ibt.dtu.dk!ut From: ut@ibt.dtu.dk (Ulf Thrane) Newsgroups: bionet.mycology Subject: Re: ?Health effects of Stachybotrys chartarum? Date: Fri, 15 Dec 1995 16:57:56 Organization: Department of Biotechnology, DTU Lines: 26 Message-ID: References: NNTP-Posting-Host: ut.ibt.dtu.dk X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] In article squash@Flairnet.com (Mike Clark) writes: >From: squash@Flairnet.com (Mike Clark) >Subject: ?Health effects of Stachybotrys chartarum? >Date: Tue, 12 Dec 1995 20:21:14 -0500 >I am a high school biology teacher seeking any information on the possible >health effects of S. chartarum. It has been positively identified in an >older middle school where at least two teachers have suffered disabling >chronic illnesses. Three species of Aspergillus were also identified at >the same location. >I would appreciate any info or suggestions as to where else to look. >My e-mail address: Mike_Clark@auburn.cnyric.org Two replies earlier this week already gave some info on this fungus. For more medical aspects as well as building construction you could try to contact Dr Suzanne Gravesen at SUG@sbi.dk Best wishes -ulf *%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%* Ulf Thrane E-mail: ut@ibt.dtu.dk Mycology Group Phone: +45 4525 2630 Department of Biotechnology - 221 +45 4593 3066 Technical University of Denmark Fax: +45 4588 4922 DK-2800 Lyngby, Denmark *%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%*%* From owner-mycology@net.bio.net Thu Dec 14 22:00:00 1995 Path: biosci!daresbury!bioftp.unibas.ch!news.vub.ac.be!usenet From: rlejeune@vnet3.vub.ac.be (Robert Lejeune) Newsgroups: bionet.mycology Subject: Enzymes/proteins commercially produced as heterologous proteins? Date: 15 Dec 1995 08:15:34 GMT Organization: VUB Brussels Lines: 11 Message-ID: <4arar6$gln@rc1.vub.ac.be> NNTP-Posting-Host: chispc2.vub.ac.be. Mime-Version: 1.0 X-Newsreader: WinVN 0.99.3 I would like to know if recently enzymes or recombinant proteins, produced as heterologous proteins in a filamentous fungus have hit the markets? I know of three such products (chymosin, phytase and lipase) and wonder if there are any new products out there. Thanks Robert lejeune Dep. Chem. Eng. Brussels From owner-mycology@net.bio.net Thu Dec 14 22:00:00 1995 Path: biosci!hawaii.edu!dorsey From: dorsey@hawaii.edu (Dr W Dorsey Stuart) Newsgroups: bionet.mycology Subject: Re: Enzymes/proteins commercially produced as heterologous proteins? Date: 15 Dec 1995 12:08:23 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 29 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <4arar6$gln@rc1.vub.ac.be> NNTP-Posting-Host: net.bio.net On Thu, 14 Dec 1995, Robert Lejeune wrote: > I would like to know if recently enzymes or recombinant proteins, produced > as heterologous proteins in a filamentous fungus have hit the markets? > I know of three such products (chymosin, phytase and lipase) and wonder if > there are any new products out there. > > Thanks > > Robert lejeune > Dep. Chem. Eng. > Brussels > Neugenesis Corporation is about to launch its first product produced in its proprietary Neurospora system. The product is a glycosylated, bioactive homodimeric human growth factor. It should be on the retail market in January, 1996. W. Dorsey Stuart President/CEO Neugenesis Corporation 2800 Woodlawn Drive Ste 251 Honolulu, Hawaii 96822 Voice (808)-539-3801 Fax (808)-539-3804 email From owner-mycology@net.bio.net Thu Dec 14 22:00:00 1995 Path: biosci!PUCCINI.CRL.UMN.EDU!paulz From: paulz@PUCCINI.CRL.UMN.EDU (Paul Zambino) Newsgroups: bionet.mycology Subject: Re: Cryphonectria parasitica Date: 15 Dec 1995 14:04:52 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <199512152203.QAA20161@puccini.crl.umn.edu> NNTP-Posting-Host: net.bio.net >PLEASE tell me all you know about >Cryphonectria parasitica !!!!! > >I am a Student here in Austria and I have >started working on this Project recently >so I need any Information I can get. >Especially I need all the Stuff about the >double stranded RNA Virus which makes it >Hypovirulent and if there are Seqences of >this RNA anywhere to get or even can send >me some cDNA Clone ?!? :-) > >Thomas Mayerhofer >Abteilung Agrarforschung und Biotechnologie >Forschungszentrum Seibersdorf >A-2444 Seibersdorf >AUSTRIA >Tel. : 02254-80-3525 >Fax : 02254-80-2118 >Email: Mayerhofer@zdfzs.arcs.ac.at You might check with Donald Nuss, Roche Institute of Molecular Biology, Dept. of Molecular Oncology and Virology, Roche Research Center, Nutley, NJ 07110, USA. He has transformed C. parasitica and the pin oak blight fungus Endothia gyrosa using cDNA from the ds RNA of the C. parasitica hypovirulence factor, with reduced virulence in the transformants. Sorry I don't have his EMAIL address. Perhaps someone else can post it to you. Paul Paul Zambino, Ph.D. USDA Forest Service Forestry Sciences Lab 5985 Highway K Rhinelander, WI 54501 PH:(715)362-1178 FAX: (715)362-1166 EMAIL: paulz@puccini.crl.umn.edu DG:p.zambino:R09F06A From owner-mycology@net.bio.net Thu Dec 14 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!news.cac.psu.edu!news.tc.cornell.edu!newsstand.cit.cornell.edu!NewsWatcher!user From: kh11@cornell.edu (Kathie T. Hodge) Newsgroups: bionet.mycology Subject: Mycology on the WWW -- changed URL Date: 15 Dec 1995 19:52:41 GMT Organization: Cornell University Lines: 15 Sender: kh11@cornell.edu (Verified) Message-ID: NNTP-Posting-Host: 128 Hello all, I recently posted to let you know about a set of web pages I maintain for the WWW Virtual Library. There you will find a pretty comprehensive listing of mycological resources on the internet. Unfortunately the URL has changed. Please update your bookmarks and links as follows: http://muse.bio.cornell.edu/~fungi/welcome.html Best wishes for the holidays, Kathie T. Hodge kh11@cornell.edu maintainer: WWW VL: Mycology From owner-mycology@net.bio.net Fri Dec 15 22:00:00 1995 Message-ID: <001315Z16121995@anon.penet.fi> Path: biosci!bloom-beacon.mit.edu!newsfeed.internetmci.com!EU.net!news.eunet.fi!anon.penet.fi Newsgroups: bionet.mycology,alt.drugs.psychedelics,alt.drugs.chemistry From: an333569@anon.penet.fi X-Anonymously-To: bionet.mycology,alt.drugs.psychedelics,alt.drugs.chemistry Organization: Anonymous forwarding service Reply-To: an333569@anon.penet.fi Date: Sat, 16 Dec 1995 00:09:19 UTC Subject: Any info on Lepiota Peele? Lines: 12 Any info on Lepiota Peele? At least that is what I think it's called: I've only seen it called "Peele's Lepiota." Anyway, info or any pointers to good articles would be appreciated. By the way, this is supposed to be a psychoactive mushroom that does NOT contain psilocybin/psilocin. Curious. --****ATTENTION****--****ATTENTION****--****ATTENTION****--***ATTENTION*** Your e-mail reply to this message WILL be *automatically* ANONYMIZED. Please, report inappropriate use to abuse@anon.penet.fi For information (incl. non-anon reply) write to help@anon.penet.fi If you have any problems, address them to admin@anon.penet.fi From owner-mycology@net.bio.net Fri Dec 15 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!nctuccca.edu.tw!netnews.ntu.edu.tw!news From: r3603207@cc.ntu.edu.tw (ccc) Newsgroups: bionet.mycology Subject: Medium for sporulation? Date: Sun, 17 Dec 1995 00:13:30 GMT Organization: National Taiwan University Lines: 10 Message-ID: <4au0ri$pmv@netnews.ntu.edu.tw> NNTP-Posting-Host: @140.112.78.181 X-Newsreader: Forte Free Agent 1.0.82 How to decide a medium for sporulation? I am doing screening experiment with Aspergillus niger. I use PDA slants to collect the spores. Recently, many strains seemed to loss their ability to sporulate, but their parent strains do sporulate. Is it too acidic for them to sporulate? I noted that the pH of the slants were very low that most of the agar slants were hydrolysed after autoclave. I even tried to add calcium carbonate into the medium but it didn't work. What should I do to make them sporulate? From owner-mycology@net.bio.net Fri Dec 15 22:00:00 1995 Newsgroups: bionet.mycology Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!torn!news.bc.net!news.express.ca!not-for-mail From: soma@express.ca (John Doe) Subject: lets get this group started-we can trade grow tipsn'shit! Message-ID: Date: Sat, 16 Dec 1995 05:50:06 GMT Organization: Pacific Royal Adventure Cruises Reply-To: soma@express.ca X-Newsreader: Forte Free Agent 1.0.82 Lines: 2 From owner-mycology@net.bio.net Sun Dec 17 22:00:00 1995 Path: biosci!agate!newsxfer.itd.umich.edu!chi-news.cic.net!newsjunkie.ans.net!prodigy.com!usenet From: ZUBP40A@prodigy.com (David Pinero) Newsgroups: bionet.mycology Subject: Re: ?Health effects of Stachybotrys chartarum? Date: 18 Dec 1995 04:04:19 GMT Organization: Prodigy Services Company 1-800-PRODIGY Lines: 13 Distribution: world Message-ID: <4b2p83$10hs@useneta1.news.prodigy.com> References: NNTP-Posting-Host: inugap5.news.prodigy.com X-Newsreader: Version 1.2 I found that Stachybotrys Chartarum was growing inside my old house. It was on a black spot on a water-damaged corner of a wall (sheetrock) in my office room, where I spent about two to four hours a day. MK-3 air samples showed that the spore content was high in that room, which was not ventilated (>1,000 p/m3; we could not isolate this fungus via Andersen viable sampling; this correlates with some literature). I had no health problems even though I was exposed to it for months before I discovered it, although it is a suspected carcinogen, so I wouldn't know for sure. However, after I found out about it I decided to move out. From owner-mycology@net.bio.net Sun Dec 17 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!usenet.eel.ufl.edu!warwick!news.shef.ac.uk!newsmaster@sheffield From: Newsgroups: bionet.mycology Subject: (no subject) Date: 18 Dec 1995 18:48:20 GMT Organization: Academic Computing Services, Sheffield University Lines: 6 Message-ID: <4b4d1k$irc@hippo.shef.ac.uk> NNTP-Posting-Host: mac107069.shef.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) X-URL: news:bionet.mycology Does anyone know how to encourage or promote the survival of a Lepiota procera mycelium (parasol). It is rare in England, but grows in my small brick greenhouse and has produced eight big fruiting bodies this year. I have never seen them before. They taste excellent. From owner-mycology@net.bio.net Mon Dec 18 22:00:00 1995 Newsgroups: bionet.mycology Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!info.ucla.edu!unixg.ubc.ca!nntp.cs.ubc.ca!news.UVic.CA!news.pfc.forestry.ca!PFC.Forestry.CA!SSINGH From: ssingh@PFC.Forestry.CA Subject: Phellinus weirii X-Nntp-Posting-Host: pfc.pfc.forestry.ca Message-ID: Sender: news@yew.pfc.forestry.ca (Usenet News) Reply-To: ssingh@PFC.Forestry.CA Organization: Forestry Canada (Pacific Forestry Centre) Date: Mon, 18 Dec 1995 18:57:41 GMT Lines: 4 I'm beginning some work with P. weirii and its infection biology by looking at molecular changes in the host roots. Has anyone worked with this organism at the molecular level (or related fungi)? I would really like to hear from you. Regards./ From owner-mycology@net.bio.net Mon Dec 18 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!swrinde!howland.reston.ans.net!news.nic.surfnet.nl!sun4nl!Utrecht.NL.net!rubens.telebyte.nl!news From: "Peter Oei (ECO Consult)" Newsgroups: bionet.mycology Subject: Re: Air Filtration and Fans Date: 19 Dec 1995 19:38:38 GMT Organization: ECO Lines: 37 Message-ID: <4b74bu$oe3@rubens.telebyte.nl> References: <4b6g0b$f06@madeline.INS.CWRU.Edu> NNTP-Posting-Host: nm-1-01.telebyte.nl Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: fa609@cleveland.Freenet.Edu fa609@cleveland.Freenet.Edu (Antonino G. Vetrano) wrote: > >Hello to all: > Can anyone shed some light or suggest some literature >to review regarding the physics of fans and filters. I wish to >equip a glovebox with a 530 cfm fan and adequate filters for >culturing. How does one figure, i.e. what are the formulae >involved, in calculating fan & filter combinations for optimum. >results. I have looked in ASHRAE manuals, spoken to refrigera- >tion techs and have dug through the local library with little >outcome. Anyone have any ideas or should I locate my ol >physics professor? > Thanks for your Assistance! >-- First you have to determine whether you want to have a real laminar flow or just a stream of filtered air. The air from a laminar flow should have a speed of about 0.45 m/s. You can calculate the surface of the filter in the following way: If a fan can blow 30 m3 per minute through a filter with a given static pressure, say 2.54cm, then the surface can be .54m2. The capacity of the fan should be somewhat higher to account for pressure losses and higher static pressure of the filter when it becomes loaded with particles. HEPA filters are sufficient. You may also want to work with a stream of clean air, which may be turbulent. In that case the velocity of the outcoming air should not be lower than the mentioned .45m/s I quoted the above from my 'Manual on mushroom cultivation' which will be republished in spring 1996. You can find more information in a book by Paul Stamets: the Mushroom Cultivator. Peter Oei From owner-mycology@net.bio.net Mon Dec 18 22:00:00 1995 Path: biosci!agate!news.ucdavis.edu!library.ucla.edu!info.ucla.edu!newsfeed.internetmci.com!howland.reston.ans.net!usenet.ins.cwru.edu!cleveland.Freenet.Edu!fa609 From: fa609@cleveland.Freenet.Edu (Antonino G. Vetrano) Newsgroups: bionet.mycology Subject: Air Filtration and Fans Date: 19 Dec 1995 13:51:07 GMT Organization: Case Western Reserve University, Cleveland OH (USA) Lines: 17 Message-ID: <4b6g0b$f06@madeline.INS.CWRU.Edu> NNTP-Posting-Host: kanga.ins.cwru.edu Hello to all: Can anyone shed some light or suggest some literature to review regarding the physics of fans and filters. I wish to equip a glovebox with a 530 cfm fan and adequate filters for culturing. How does one figure, i.e. what are the formulae involved, in calculating fan & filter combinations for optimum. results. I have looked in ASHRAE manuals, spoken to refrigera- tion techs and have dug through the local library with little outcome. Anyone have any ideas or should I locate my ol physics professor? Thanks for your Assistance! -- |-B^{> oB^{> dB^{> * -\B^{?> >B^{!> P^{> {B^{> ;^{> | A. G. Vetrano, fa609@cleveland.freenet.edu | Specialization | | is for Insects |<}c8 <}^| <}^O <}^: References: <4b2p83$10hs@useneta1.news.prodigy.com> NNTP-Posting-Host: uga.cc.uga.edu (David Pinero) writes: > I found that Stachybotrys Chartarum was growing inside my old house. >It was on a black spot on a water-damaged corner of a wall (sheetrock) in >my office room, where I spent about two to four hours a day. MK-3 air >samples showed that the spore content was high in that room, which was >not ventilated (>1,000 p/m3; we could not isolate this fungus via >Andersen viable sampling; this correlates with some literature). Stachybotrys can be isolated using the Andersen Sampler. I've seen it recovered on Rose Bengal and Malt extract media. > I had no health problems even though I was exposed to it for months >before I discovered it, although it is a suspected carcinogen, so I >wouldn't know for sure. However, after I found out about it I decided to >move out. I think that this is a good example of Stachybotrys having an over exaggerated importance in building air quality. Proper hygiene ie removal of the water damaged material probably would have reduced exposure risks to near zero. Repairing the water damageshould have be the building owner's biggest concern. Michael From owner-mycology@net.bio.net Tue Dec 19 22:00:00 1995 Path: biosci!agate!kosh-321-1.berkeley.edu!dgeiser From: David Geiser Newsgroups: bionet.mycology Subject: Re: Medium for sporulation? Date: 20 Dec 1995 07:11:11 GMT Organization: University of California at Berkeley Lines: 29 Distribution: world Message-ID: <4b8cuf$jcf@agate.berkeley.edu> References: <4au0ri$pmv@netnews.ntu.edu.tw> NNTP-Posting-Host: kosh-321-1.berkeley.edu Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Newsreader: Nuntius 2.0.4_68K X-XXMessage-ID: X-XXDate: Tue, 19 Dec 1995 23:10:48 GMT In article <4au0ri$pmv@netnews.ntu.edu.tw> ccc, r3603207@cc.ntu.edu.tw writes: > How to decide a medium for sporulation? > I am doing screening experiment with Aspergillus niger. I use PDA >slants to collect the spores. Recently, many strains seemed to loss >their ability to sporulate, but their parent strains do sporulate. > Is it too acidic for them to sporulate? I find that wild Aspergillus niger isolates sporulate quite well on the standard Aspergillus minimal medium (MM), the formula for which you can find at the Fungal Genetics Stock Center Web Site, or in the following reference: Barratt et al., 1965 Genetics 52:233-246. In fact, there have been times when I wished they were a little less enthusiastic. Adjust the pH of the medium to 6.5 prior to autoclaving. Complete medium (CM) works even better for most species, in my experience. Better sporulation might also be supported on Petri Plates. The problem is that it is much messier trying to harvest the spores. To do this, I add 10 ml of sterile 0.08% Tween80 to the top of the plate, scrape with a tool I call a "lawnmower", and then collect the spore suspension. To make a lawnmower, simply twist some inoculating wire into a triangle shape and place it in an inoculating loop handle. Use the flat end of the triangle to scrape the plate surface. This is something you would want to do in a sterile hood. Dave Geiser From owner-mycology@net.bio.net Tue Dec 19 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: stamets1@aol.com (Stamets 1) Newsgroups: bionet.mycology Subject: filters Date: 20 Dec 1995 09:55:36 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 29 Sender: root@newsbf02.news.aol.com Message-ID: <4b9858$d3u@newsbf02.news.aol.com> References: <4b6g0b$f06@madeline.INS.CWRU.Edu> Reply-To: stamets1@aol.com (Stamets 1) NNTP-Posting-Host: newsbf02.mail.aol.com Antonio et al. - It is actually quite simple. The goal: 100-200 ft./minute air velocity. This velocity will prevent air borne contaminants from descending into your work area. Take the CFM of a fan at 1" of static pressure. Manufacturers will list the net CFM's at various pressure drops, usually rated as S.P. (= static pressure of resistance = the pressure necessary to move a 1" diameter of water 1 " in a column.) Let's say your fan has 400 CFM at 1" S.P. and you have 24 x 24 inch. HEPA filter. 400 cfm/4 sq. ft = 100 feet per minute air velocity. In effect, this combination would give you the minimum velocity. As particulates build up in the filter, so too its resistance. I would recommend either reducing the size of the filter by a factor of 2 or increase the CFM of the fan. These type of formulas are described in Growing Gourmet & Medicinal Mushrooms ( the second edition of which is available in January) as well as drawings for converting glove boxes into laminar flow hoods. Unless the outer edge of air is recirculated back into the air plenum, you will likely have two small dead air pockets located about 1/3 from the outer periphery of the filter face. This does not pose any significant risk and is the trade-off involved in making your own. Good luck. paul stamets From owner-mycology@net.bio.net Wed Dec 20 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!usenet.eel.ufl.edu!news.ultranet.com!usenet From: griner@ultranet.com (George Riner) Newsgroups: bionet.mycology Subject: Truffle article in Natural History 1/96 Date: Thu, 21 Dec 1995 02:55:49 GMT Organization: UltraNet Communications, Inc. Lines: 3 Message-ID: <30d8ccb0.4029168@news.ultranet.com> NNTP-Posting-Host: gar.ultranet.com X-Newsreader: Forte Agent .99c/16.141 The 1/96 issue of Natural History magazine has an article on hunting the Perigord truffle in France. A quick read and an interesting note about looking for them by following a certain kind of insect. From owner-mycology@net.bio.net Wed Dec 20 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!ix.netcom.com!netcomsv!uu3news.netcom.com!netcomsv!uu4news.netcom.com!gatekeeper.genencor.com!usenet From: cwang@genencor.com (cynthia wang) Newsgroups: bionet.mycology Subject: prerservation of pleurotus Date: 20 Dec 1995 23:06:38 GMT Organization: Genencor Intl. Inc Lines: 4 Sender: -Not-Authenticated-[7114] Message-ID: <4ba4tu$gg8@gatekeeper.genencor.com> NNTP-Posting-Host: 10.1.1.96 X-Posted-From: InterNews 1.0.6@cwang.genencor.com Xdisclaimer: No attempt was made to authenticate the sender's name. I am trying to preserve the mycelia of Pleurotus ostreatus. I tried to freeze the culture broth/suspension but the revival rate was very low and it takes 3 weeks to recover the culture from frozen to an agar culture. Any suggestions? From owner-mycology@net.bio.net Wed Dec 20 22:00:00 1995 Message-ID: <055327Z21121995@anon.penet.fi> Path: biosci!daresbury!nntp-trd.UNINETT.no!Norway.EU.net!EU.net!news.eunet.fi!anon.penet.fi Newsgroups: alt.drugs,alt.drugs.psychedelics,rec.drugs.psychedelics,bionet.mycology From: an333569@anon.penet.fi X-Anonymously-To: alt.drugs,alt.drugs.psychedelics,rec.drugs.psychedelics,bionet.mycology Organization: Anonymous forwarding service Reply-To: an333569@anon.penet.fi Date: Thu, 21 Dec 1995 05:52:35 UTC Subject: Lepiota Peele or Lepiota Memorii ??? Lines: 11 Anyone have any info on these lepiota? I can not find ANY reference to them anywhere! I looked thru the Science Citations Index back to 1968 and have searched EVERY data base I can (psych-lit, medline, etc,etc,etc,etc)! Do these fungi even exist at all or did my original reference simply misidentify some other fungi? --****ATTENTION****--****ATTENTION****--****ATTENTION****--***ATTENTION*** Your e-mail reply to this message WILL be *automatically* ANONYMIZED. Please, report inappropriate use to abuse@anon.penet.fi For information (incl. non-anon reply) write to help@anon.penet.fi If you have any problems, address them to admin@anon.penet.fi From owner-mycology@net.bio.net Wed Dec 20 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!news.uiowa.edu!usenet From: michael-clark@uiowa.edu (Michael Clark) Newsgroups: bionet.mycology Subject: Re: prerservation of pleurotus Date: 21 Dec 1995 02:57:30 GMT Organization: University of Iowa, Iowa City, IA, USA Lines: 28 Sender: -Not-Authenticated-[6705] Distribution: world Message-ID: <4baieq$v8c@flood.weeg.uiowa.edu> References: <4ba4tu$gg8@gatekeeper.genencor.com> NNTP-Posting-Host: russo-lab.physlog.uiowa.edu X-Posted-From: InterNews 1.0.1@russo-lab.physlog.uiowa.edu Xdisclaimer: No attempt was made to authenticate the sender's name. Cynthia Wang wrote: >I am trying to preserve the mycelia of Pleurotus ostreatus. I tried to >freeze the culture broth/suspension but the revival rate was very low >and it takes 3 weeks to recover the culture from frozen to an agar >culture. Any suggestions? Did you use any cryoprotectant? The standard for yeast work is 10% glycerol. You could also try 10% DMSO, which is what we use for freezing mammalian cells. These agents dramatically improve survival of frozen microorganisms. Also, what temperature are you freezing at? The colder the better. -70C is the standard for long term cryopreservation of bacteria and yeast. Survival is reduced at -20C. BTW: slow freezing works better than flash freezing (i.e. immersion into liquid N2). Or it may be that Pleurotus just doesn't freeze well. I've successfully frozen at -70C, with excellent recovery, both Coprinus comatus and Stropharia ruguloso-annuata in 10% glycerol. But I'm still working to freeze morel mycelium (any suggestions would be helpful, hint hint to anyone in the know!) --Michael Clark --University of Iowa --Molecular Biology Program From owner-mycology@net.bio.net Wed Dec 20 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!EU.net!peer-news.britain.eu.net!lyra.csx.cam.ac.uk!virol1.plantsci.cam.ac.uk!user From: ddp10@cus.cam.ac.uk Newsgroups: bionet.mycology Subject: MYCOLOGIST POSITION Date: Thu, 21 Dec 1995 11:38:44 +0100 Organization: . Lines: 4 Message-ID: NNTP-Posting-Host: virol1.plantsci.cam.ac.uk The Department of Plant Sciences, University of Cambridge, England is seeking to employ a Lecturer (equivalent to Assistant professor in the US) in Molecular Mycology-----for more details contact Katherine Brown (klb1003@cus.cam.ac.uk) From owner-mycology@net.bio.net Wed Dec 20 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!uwm.edu!reuter.cse.ogi.edu!engr.orst.edu!news.PEAK.ORG!microbe From: microbe@PEAK.ORG (Steven Carpenter) Newsgroups: bionet.mycology Subject: Re: Air Filtration and Fans Date: 20 Dec 1995 13:51:50 GMT Organization: CS Outreach Services, Oregon State University, Corvallis, OR, USA Lines: 70 Message-ID: <4b94dm$id0@odo.PEAK.ORG> References: <4b6g0b$f06@madeline.INS.CWRU.Edu> NNTP-Posting-Host: peak.org X-Newsreader: TIN [version 1.2 PL2] If you want to equip a glovebox (versus building a high tech cleanroom) you have two concerns: 1. Optimization of airflow to reduce eddies 2. Personal safety In a glovebox, you want to direct the airflow in steady streams over and away from the work surface. If you ramp up the fan too much, you will get turbulence which potentially rams air against the work surface (such as an open Petri plate) and back upwards in a roil. You don't want that, as the air that could roil over an open plate of sporulating fungi could re-deposit spores onto surfaces of sterile tubes and plates. So...you could calculate all you want, but you can perform a VERY simple test: 1. Put a rheostat on the fan motor 2. Turn on the fan and fog the insides with a CO2 fogger. You can make a fogger using a container of hot water, and dump a chunk of dry ice into the container. An old plug-in coffee percolator works fine, and if the pour spout is small enough, you can run tubing from the pour spout into the glove box. Once you are fogging into the chamber, you can adjust the fan to reduce turbulence. Turbulence can also be reduced or completely eliminated by putting slots along the sides of the glove box, and having a sliding damper over the slots to adjust the opening (and also to completely close the damper when the fan is turned off to keep down unwanted contamination). We have successfully used these techniques in "minienvironments" in high technology semiconductor cleanroom equipment. Our findings show that reduced airflow and slotting works well: often the most effective airflows are around 35-45 feet/min. Personal Safety: if you are working with any hazardous microbes, this is not a safe system. It blows propagules right into your face. Protocol: Finally, just blowing clean air into the chamber in a controlled fashion won't do it all for you. The biggest sources of contamination in a glovebox system will be on the surfaces of whatever material you bring into the box from the outside, including your hands and arms. Be sure to have a pre-cleaning protocol for materials entering the box, otherwise that clean air will blow the propagule-laden dirt/skin flakes from your hands onto the surface of your cultures. -Steven E. Carpenter, Ph.D. Cascade Research Associates & Abbey Lane Laboratory microbe@peak.org Antonino G. Vetrano (fa609@cleveland.Freenet.Edu) wrote: : Hello to all: : Can anyone shed some light or suggest some literature : to review regarding the physics of fans and filters. I wish to : equip a glovebox with a 530 cfm fan and adequate filters for : culturing. How does one figure, i.e. what are the formulae : involved, in calculating fan & filter combinations for optimum. : results. I have looked in ASHRAE manuals, spoken to refrigera- : tion techs and have dug through the local library with little : outcome. Anyone have any ideas or should I locate my ol : physics professor? : Thanks for your Assistance! : -- : |-B^{> oB^{> dB^{> * -\B^{?> >B^{!> P^{> {B^{> ;^{> : | A. G. Vetrano, fa609@cleveland.freenet.edu | Specialization : | | is for Insects : |<}c8 <}^| <}^O <}^: References: <17479DF1BS86.BLACKMJ@UGA.CC.UGA.EDU> Reply-To: davidmarc@aol.com (DavidMarc) NNTP-Posting-Host: newsbf02.mail.aol.com >I think that this is a good example of Stachybotrys having an over exaggerated importance in building air quality. It is refreshing to see such reason! Stachy is not so uncommon and I appreciate your reasoning on the relative risks and the buildin owners focus on remediation of the water-damage rather than on evacuation of occupants not at risk. Uncommon sense I must say and commend! Davidmarc@aol.com From owner-mycology@net.bio.net Thu Dec 21 22:00:00 1995 Path: biosci!ihnp4.ucsd.edu!swrinde!newsfeed.internetmci.com!EU.net!Norway.EU.net!telepost.no!usenet From: Roy Markussen Newsgroups: bionet.mycology Subject: FIELDTRIP TO NEW ZEALAND Date: 22 Dec 1995 10:27:12 GMT Organization: TelePost Public Access Lines: 18 Message-ID: <4be160$br3@nms.telepost.no> NNTP-Posting-Host: oslo412.telepost.no Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 1.1 (Windows; I; 16bit) I'm a Norwegian amateur mycologist. Does anyone have any tips for a fieldtrip to New Zealand? My main interrests are; - Macrofungi - Photographing (dias) Please contact on this address, or my private adress; Per Marstad Postmannsveien 7 3103 Třnsberg Norway From owner-mycology@net.bio.net Thu Dec 21 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!EU.net!Germany.EU.net!news.dfn.de!news.uni-jena.de!news From: Johannes Woestemeyer Newsgroups: bionet.mycology Subject: Assistantship in Mycology Date: 22 Dec 1995 07:42:57 GMT Organization: FSU Jena: Inst. Gen. Microbiol. / Microbe Genetics Lines: 21 Message-ID: <4bdni1$32a@fsuj01.rz.uni-jena.de> NNTP-Posting-Host: pc1.mikrogen.uni-jena.de Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) Hello Mycologists! The Institute for General Microbiology and Microbe Genetics at Friedrich-Schiller-Universitaet Jena /Germany looks for at least one, possibly two assistants with some background in mycology. We will be glad to welcome colleagues at the postdoctoral level who like to look at fungi under organismic points of view. They must have experience in isolating and determining fungi from the rhizosphere and perhaps endophytic organisms, should be interested in secondary metabolites and must be open to cooperation with colleagues working in the field of molecular strain typing. In few words: We need scientists devoted to evolutionary biology of fungi with some regards to applied aspects. Applicants will have to take an active part in our teaching responsibilities in microbiology. Interested? If yes, please contact me: Joh. Woestemeyer, b5wojo@rz.uni-jena.de or Fax: +49 (3641) 630321 From owner-mycology@net.bio.net Thu Dec 21 22:00:00 1995 Newsgroups: bionet.mycology Path: biosci!agate!howland.reston.ans.net!newsxfer.itd.umich.edu!nntp.cs.ubc.ca!news.UVic.CA!news.pfc.forestry.ca!PFC.Forestry.CA!RWINDER From: rwinder@PFC.Forestry.CA (Richard Winder) Subject: Re: Medium for sporulation? X-Nntp-Posting-Host: pfc.pfc.forestry.ca Message-ID: Sender: news@yew.pfc.forestry.ca (Usenet News) Reply-To: rwinder@PFC.Forestry.CA Organization: Forestry Canada (Pacific Forestry Centre) References: <4au0ri$pmv@netnews.ntu.edu.tw> Date: Wed, 20 Dec 1995 17:39:09 GMT Lines: 26 In article <4au0ri$pmv@netnews.ntu.edu.tw>, r3603207@cc.ntu.edu.tw (ccc) writes: > How to decide a medium for sporulation? > I am doing screening experiment with Aspergillus niger. I use PDA >slants to collect the spores. Recently, many strains seemed to loss >their ability to sporulate, but their parent strains do sporulate. > Is it too acidic for them to sporulate? I noted that the pH of the >slants were very low that most of the agar slants were hydrolysed >after autoclave. I even tried to add calcium carbonate into the medium > >but it didn't work. What should I do to make them sporulate? > One method I've used that works is to autoclave the medium for twice the normal time (for me, 40 minutes). I'm not sure of the reason- caramelization of sugars?- but it seems to work particularly well with V-8 juice agar, or V-8 Juice/ corn leaf decoction mixtures. I work with weed pathogens- I find the technique works for a broad range of foliar fungi, contaminants, opportunists, etc., including Aspergillus. I don't find PDA to be very suitable for maintaining sporulation. Although pH may have some effect, I tend to wory about the Carbon/Nitrogen ratio of the medium, and the total amount, complexity, and variety of carbohydrates used as a nutrient source. -RSW RICHARD WINDER Title: Research Scientist Canadian Forest Service Phone: (604) 363-0773 Victoria, B.C. Internet: RWINDER@A1.PFC.Forestry.CA From owner-mycology@net.bio.net Fri Dec 22 22:00:00 1995 Path: biosci!agate!news.duke.edu!solaris.cc.vt.edu!homer.alpha.net!uwm.edu!vixen.cso.uiuc.edu!news.ksu.ksu.edu!news.mid.net!news.dra.com!news.getnet.com!news.dgsys.com!sloth.swcp.com!tesuque.cs.sandia.gov!lynx.unm.edu!somasf.unm.edu!lus From: lus@taxus.oesa.ufz.de,InterGate,InterGate Newsgroups: bionet.mycology Subject: Re: South American Field Guide Date: Tue, 12 Dec 1995 17:54:47 GMT Organization: UNMSOM Lines: 49 Message-ID: NNTP-Posting-Host: somasf.unm.edu Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit From: lus@taxus.oesa.ufz.de,InterGate Subject: Re: South American Field Guide Date: Tue, 12 Dec 1995 17:54:47 GMT From: lus@taxus.oesa.ufz.de (Nobert Luschka) Subject: Re: South American Field Guide Date: 12 Dec 1995 17:54:47 -0000 >In article <4aj00q$29h@newshost.lanl.gov>, 74627.3370@compuserve.com >says... >> >>I would appreciate any suggestions for a good field guide for southern >>South America, preferably in English? >> >>Thank you. >> >>Ted Stampfer >> > One possibility of a field-key at least for Agaricales is "J. Raithelhuber: Flora mycologia Argentina. Hongos III." Metrodiana 1991. It is in spanish with an english key (156 p.) Norbert Luschka Dr. Nobert Luschka Sektion Biozoenoseforschung (Dep. of Community Ecology) Umweltforschungszentrum Leipzig-Halle GmbH (Centre for Environmental Research Leipzig-Halle Ltd.) Hallesche Strasse 44 D-06246 Bad Lauchstaedt, Germany Tel.:(+34635)73-291 Fax: (+34635)73-237 E-Mail: lus@oesa.ufz.de Path: lynx.unm.edu!jobone!newsxfer.itd.umich.edu!newsfeed.internetmci.com!EU.n et!Norway.EU.net!nntp-oslo.UNINETT.no!nntp-trd.UNINETT.no!daresbury!not- for-mail Path: lynx.unm.edu!somasf.unm.edu!lus Mime-Version: 1.0 Content-Transfer-Encoding: 8bit Content-Type: text/plain; charset=ISO-8859-1 From owner-mycology@net.bio.net Fri Dec 22 22:00:00 1995 Path: biosci!agate!newsxfer2.itd.umich.edu!newsxfer.itd.umich.edu!tank.news.pipex.net!pipex!howland.reston.ans.net!vixen.cso.uiuc.edu!news.ksu.ksu.edu!news.mid.net!news.dra.com!news.getnet.com!news.dgsys.com!sloth.swcp.com!tesuque.cs.sandia.gov!lynx.unm.edu!som