From owner-peptides@net.bio.net Tue Jul 01 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Phil Newsgroups: bionet.molecules.peptides Subject: Using Copper Peptide as hair growth stimulator Date: 2 Jul 1997 09:31:43 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 6 Sender: daemon@net.bio.net Approved: cmgross@biosci.cbs.umn.edu Distribution: world Message-ID: <5pdvpf$40u@net.bio.net> NNTP-Posting-Host: net.bio.net Super Oxide Dismustases are known to have hair stimulating properties, my question is would it be possible for a layman such as myself to synthesize a copper peptide for topical skin application? What would I need and how would I do it. What would be the toxicity of this be? Thanks From owner-peptides@net.bio.net Tue Jul 01 23:00:00 1997 Path: biosci!biosci!not-for-mail From: benvdp@innogenetics.be Newsgroups: bionet.molecules.peptides Subject: Phosphopeptides Date: 2 Jul 1997 09:32:04 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Approved: cmgross@biosci.cbs.umn.edu Distribution: world Message-ID: <5pdvq4$41n@net.bio.net> NNTP-Posting-Host: net.bio.net We have great difficulties in separating phospho and non-phospho peptides. We have used different columns and buffer systems. In many cases the two forms will co-elute or have appr. equal retention times. Can anyone help give us any new ideas ? Thank you very much! From owner-peptides@net.bio.net Sat Jul 05 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Phillip Robinson Newsgroups: bionet.molecules.peptides Subject: Re: Phosphopeptides Date: 6 Jul 1997 13:06:15 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Approved: cmgross@biosci.cbs.umn.edu Distribution: world Message-ID: <5potrn$lde@net.bio.net> NNTP-Posting-Host: net.bio.net You might try IMAC using Fe immobilised to the column. This gives extremely high affinity for phosphate in proteins and might work well for some of your peptides. Of course, like all good ideas, for some reason it doesn't work well for all (or even most) proteins. eg: Erickson, A.C. & Johnson, G.V.W. (1993) Metal (Fe3+) affinity chromatography: Differential adsorption of tau phosphoproteins. J. Neurosci. Methods 46, 245-249. Phil benvdp@innogenetics.be wrote: > > We have great difficulties in separating phospho and non-phospho peptides. > We have used different columns and buffer systems. In many cases the two > forms will co-elute or have appr. equal retention times. Can anyone help > give us any new ideas ? > > Thank you very much! From owner-peptides@net.bio.net Sun Jul 06 23:00:00 1997 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.molecules.peptides Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 7 Jul 1997 12:06:56 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 234 Sender: daemon@net.bio.net Approved: cmgross@biosci.cbs.umn.edu Distribution: world Message-ID: <5preog$r89@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-peptides@net.bio.net Thu Jul 24 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "Georgi P. Gladyshev" Newsgroups: bionet.molecules.peptides Subject: thermodynamics of evolution of peptides Date: 25 Jul 1997 13:28:31 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 89 Sender: daemon@net.bio.net Approved: cmgross@biosci.cbs.umn.edu Distribution: world Message-ID: <5rb29f$svt@net.bio.net> NNTP-Posting-Host: net.bio.net Subject: Supramolecular chemistry and thermodynamics of biological evolution, Thermodynamics of origin of life, Macrothermodynamics, Self-assembly of supramolecular structures, Supramolecules and Assemblies, Adaptation, Aging Dear Colleagues, My Institute: URL http://www.biograph.comstar.ru/evolution/ The information in my URL is: Hello to all, I am seeking professional scientists who would like to understand (to comprehend) my thermodynamic theory of the Evolution of Living Beings. This theory is presented in work: G. P. Gladyshev, "Thermodynamic theory of the Evolution of Living Beings" (in Russian, Moscow, 1996). The work will be published by NOVA Sci. Publ., Inc., NY USA. I can send an additional information. For best understanding of the work it is very imported to have the experience in fields: classical physical chemistry, biophysical chemistry, chromatography, biological evolution, ontogenesis, aging, DSC of tissues, ... . Please, respond to e-mail: biograph@online.comstar.ru or academy@endeav.msk.su or mail: Academy of Creative Endeavors, 36, Novy Arbat, Moscow, 121205, Russia. Many thanks in advance! Georgi P. Gladyshev Professor of Physical Chemistry (Institute of Ecological Biophysical Chemistry of the Academy of Creative Endeavors, Laboratory of Thermodynamics and Macrokinetics of Non-equilibrium Systems of N.N.Semionov Institute of Chemical Physics of the Russian Academy of Sciences), Professor of the Chair of Biomedical Systems and Devices (N.E.Bauman Moscow State Technical University, 1975-1985) Summary This book is devoted to the physical theory of the biological evolution. The theory is based on macrothermodynamics, i.e., the hierarchic thermodynamics of complex systems. The results of the studies presented in the book allow one to state that the peculiarities of the evolution of living beings, as well as the peculiarities of the chemical evolution, can be explained without the concepts of the dynamical self-organization and the dissipative structures. According to the second law, the tendency of the evolution of biological systems on chemical and supramolecular levels can be determined by studying the effect of thermodynamical self-organization (self-assembly). The criterion for estimating the evolutionary development of supramolecular structures of biosystems (biotissues) is given by the variation of the specific Gibbs function of their formation. During the processes of ontogenesis, philogenesis, and biological evolution in general, the specific supramolecular component of the Gibbs function of a biosystem, that is quasi-closed thermodynamically and kinetically, tends to a relative minimum. The value of this minimum is a characteric of the given biosystem surrounded by the given environment. The non-stationary theoretical model presented in the monograph explains the reasons causing the variations in the chemical composition and structure of living beings in the course of ontogenesis, philogenesis, and the evolution in general. It also allows to find out the rules determining the variations in the composition and structure of a biosystem during its adaptation to the external conditions. At present, it seems that the most essential applications of the theory relate to the study of the living creatures behavior and to gerontology, pharmacology, nutrition. The book is written as an interdisciplinary monograph and is designed for a reader with university education. During the last decades, an opinion has widely spread that there is "the apparent contradiction between biological order and laws of physics - particularly the second law of thermodynamics". Besides, it is claimed that this contradiction "cannot be removed as long as one tries to understand living systems by the methods of equilibrium thermodynamics". The author of the present work states: if living systems are described in the framework of hierarchic equilibrium thermodynamics, this contradiction does not exist. Review of the work will appear in one of the next issues of Journal of Biological Physics. See also: G.P.Gladyshev, "Thermodynamic Direction of Biological Evolution: Model and Reality", Biology Bulletin, Vol. 23, No. 4, 1996, pp. 315-322. Translated from Izvestia Akademii Nauk, Seriya Biologicheskaya, No. 4, 1996, pp. 389-397. I have an suggestion: introduce to your Web site the section "Thermodynamics of Aging" . Sincerely, Georgi Gladyshev. From owner-peptides@net.bio.net Thu Jul 24 23:00:00 1997 Path: biosci!biosci!not-for-mail From: RHODES@UCONNVM.UCONN.EDU Newsgroups: bionet.molecules.peptides Subject: 96 well plates ?? Date: 25 Jul 1997 13:31:09 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Approved: cmgross@biosci.cbs.umn.edu Distribution: world Message-ID: <5rb2ed$t2k@net.bio.net> NNTP-Posting-Host: net.bio.net Does anyone know of a source for 96 well (or 6 or 12 or 24 or 384...) plates made of something OTHER than plastic? Thanks! | O==O | | David G. Rhodes O==O Phone 860-486-5413 | | School of Pharmacy; U-92 O==O Fax 860-486-4998 | | University of Connecticut O==O | | Storrs, CT 06269-2092 O==O rhodes@uconnvm.uconn.edu | |_____________________________O==O____________________________| CAUTION: Dates on calendar are closer than they appear! From owner-peptides@net.bio.net Tue Jul 29 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Genetics Department Newsgroups: bionet.molecules.peptides Subject: Re: Phosphopeptides Date: 30 Jul 1997 13:44:59 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 6 Sender: daemon@net.bio.net Approved: cmgross@biosci.cbs.umn.edu Distribution: world Message-ID: <5ro94b$8nn@net.bio.net> NNTP-Posting-Host: net.bio.net Personnaly I separated phospho from non phosphorylated peptide on ion exchange column. In my case the two form present a pI with more than two unit differenceand make things quite easy. Sincerely, Christophe