From owner-peptides@net.bio.net Tue Mar 02 22:00:00 1999 Path: biosci!biosci!not-for-mail From: Michael Sherrell Newsgroups: bionet.molecules.peptides Subject: ABI 377 for sale Date: 3 Mar 1999 08:44:17 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 39 Sender: daemon@net.bio.net Approved: cmgross@biosci.cbs.umn.edu Distribution: world Message-ID: <7bjot1$151@net.bio.net> NNTP-Posting-Host: net.bio.net Newly for sale: ABI 377 DNA sequencer, XL, 96 lanes, under transferrable Perkin Elmer service contract until March 2000. Price: $105,000. I also have these peptide and oligo synthesizers and sequencers, LC-mass specs and NMRs for sale: Synthesizers and sequencers: ABI 394 synthesizer, valve blocks rebuilt, warranteed: $12,500          ABI Procise 492 protein sequencer, $59,000 ABI 3948 synthesizer: inquire ABI 390Z synthesizer, 50-100uM yields: $4,000        ABI 373 stretch, 5-filter, Genescan, under ABI service: $19,500 Li-Cor 4000LS, 1994 model: <$20,000 ABI 430, working: $6,000 ABI 430, rebuilt, warranteed: $10,500 ABI 433, upgraded by ABI from 431: $39,000 Advanced ChemTech 396 Multiple Biomolecule Synthesizer, unused: $45,000 PerSeptive 9050+, $6,000 + a variety of other ABI, PerSeptive, HP, Biosearch, ACT, etc. synthesizers and sequencers LC-MS: PE Sciex API III+, 5yrs old, ES, APCI (installation available): $82,000 Finnigan TSQ 7000, installed, warranty: $149,000      Finnigan TSQ 700, 1995; NOT Y2K compliant; under Finnigan service now: $80,000 HP 5989B, extended mass range (2000 amu), hex ion guide (HPLC, installation & warranty available): $45,000 NMRs: Bruker AMX-500, installed: $175,000 Bruker AC-250, running in lab now: $30,000 Varian Gemini 300: $70,000 . Please contact me to discuss any of these, or if you have any items you might like to sell. Michael Sherrell Grizzly Analytical 707 887 2919/fax 707 887 9834 www.grizzlyanalytical.com From owner-peptides@net.bio.net Sun Mar 07 22:00:00 1999 Path: biosci!biosci!not-for-mail From: The Antibody Resource Page Newsgroups: bionet.molecules.peptides Subject: The Antibody Resource Page Date: 8 Mar 1999 08:28:18 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 52 Sender: daemon@net.bio.net Approved: cmgross@biosci.cbs.umn.edu Distribution: world Message-ID: <7c0tr2$hnd@net.bio.net> NNTP-Posting-Host: net.bio.net The Antibody Resource Page (http://www.antibodyresource.com/) is your number one source for information on antibodies. Here is just some of what can be found on the page: 1. Custom Antibodies - a list of online companies that will make custom antibodies for you. 2. Books - a small but growing section on books important to researchers who work with antibodies. Do you sell such books? Let us know and we can post a review. 3. How to Find an Antibody - a variety of ways on and off the web to find the antibody you are looking for. There are links to free search engines that allow you to peruse a multitude of companies for the specific antibody that you need. 4. Online Companies - links to over 130 companies that sell antibodies or antibody related products. Is your company listed on this page? 5. Antibody Image Gallery - see some of the latest in animated and non-animated antibody graphics 6. Bulletin Board - Have a question? Then stop by and post a message. Or answer a question. 7. Educational Resources - a variety of new links have been added.There are links to pages on immunochemistry, antibody production, autoimmunity, vaccines, immunology and much more. This page is divided up into sections on research, educational, and health resources. 8. The latest in antibody news - Get up-to-date, antibody-related news articles and newsgroup discussions 9. Online databanks and databases - links to online protein and nucleic acid sequencing databases. Invaluable to biochemists and molecular biologists who work with antibodies. Check it all out at: http://www.antibodyresource.com/ ps. Don't forget to visit our current sponsors, Diatec (http://www.diatec.com/), Sciquest (http://www.sciquest.com/) and Bethyl Laboratories (http://www.bethyl.com). pps. Are you interested in being a sponsor on the Antibody Resource Page (ARP)? Nearly half of the visitors come to find commercially available antibodies. If you are a company that sells antibodies and want to increase webtraffic to your site, email the antibody resource page to find out about sponsorship: antibody@antibodyresource.com From owner-peptides@net.bio.net Sun Mar 07 22:00:00 1999 Path: biosci!biosci!not-for-mail From: Pier Carlo Montecucchi <100143.765@CompuServe.COM> Newsgroups: bionet.molecules.peptides Subject: Biotech Products Date: 8 Mar 1999 08:47:51 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Approved: cmgross@biosci.cbs.umn.edu Distribution: world Message-ID: <7c0uvn$jog@net.bio.net> NNTP-Posting-Host: net.bio.net Do you need outsourcing services in biotech areas? We can assist you. Contact: MonteGen - A Bureau for Biotechnology Email: innovations@montegen.com (1) 305-776-2241 internationally --  From owner-peptides@net.bio.net Thu Mar 11 22:00:00 1999 Path: biosci!biosci!not-for-mail From: Ben Vendeland Newsgroups: bionet.molecules.peptides Subject: Workshop on Single Particle Reconstruction from Electron Date: 12 Mar 1999 14:54:40 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 32 Sender: daemon@net.bio.net Approved: cmgross@biosci.cbs.umn.edu Distribution: world Message-ID: <7cc5vg$k9l@net.bio.net> NNTP-Posting-Host: net.bio.net The Pittsburgh Supercomputing Center will offer a workshop on Single Particle Reconstruction from Electron Microscope Images on July 21-24, 1999. This workshop will cover techniques of 3D reconstruction of single biological macromolecules imaged by cryo-electron microscopy, programming on the level of SPIDER scripts, and efficient use of parallel processing. The following topics will be included: theory of EM image formation, projection theorem, sampling, alignment and classification, determination of orientations, 3D reconstruction and refinement, CTF Correction, visualization and interpretation. Practical sessions will be devoted to EM image processing using SPIDER system ported on supercomputers. Special topics will include reconstruction of symmetric macromolecules and introduction to EMAN, an automated processing system. Instructors for this workshop include Prof. Joachim Frank, Prof. Wah Chiu, Dr. Pawel Penczek, Dr. Steve Ludtke, Dr. Philip R. Baldwin and Dr. Christian Spahn. To register for this workshop, or for more information, please check the following URL: http://www.psc.edu/biomed/biomed.html From owner-peptides@net.bio.net Mon Mar 29 23:00:00 1999 Path: biosci!biosci!not-for-mail From: Per Thoren Newsgroups: bionet.molecules.peptides Subject: How do I prevent peptide adsorption to surfaces?? Date: 30 Mar 1999 11:13:01 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Approved: cmgross@biosci.cbs.umn.edu Distribution: world Message-ID: <7dr7nt$26o@net.bio.net> NNTP-Posting-Host: net.bio.net I am doing fluorscence studies on a peptide, but the amount of peptide that sticks to the cuvette walls and even the polypropylene pipette tips is not negligible. Are there any modified cuvettes commercially available? Are there any good methods for modifying quartz cuvettes, to make them "non-sticky"? We have tried GelSlick, but it didn't do the job. Are there any modified commercially available pipette tips? I am grateful for any help you can give me Per Thorén From owner-peptides@net.bio.net Wed Mar 31 23:00:00 1999 Path: biosci!biosci!not-for-mail From: "Graham Dellaire, Ph.D." Newsgroups: bionet.molecules.peptides Subject: Problems expressing a 50-60kDa protein in E. coli... tips? Date: 1 Apr 1999 10:03:24 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 39 Sender: daemon@net.bio.net Approved: cmgross@biosci.cbs.umn.edu Distribution: world Message-ID: <7e0cdc$if3@net.bio.net> NNTP-Posting-Host: net.bio.net Dear all, I am trying to express a 1.65 kb cDNA in E. coli using the pET32+ system with BL21(DE3) bugs. The protein including the trxa/his tag should be about 50 to 60 kDa. The protocol I used is as follows: first I grow up a 5 ml culture overnight from a single colony; then in the morning to inoculate 10 mls of medium with 100 ul of the o/n culture; two hours later I start induction with 1 mM IPTG and collect samples at 1 h, 2 hr, 4 hrs post-induction and o/n. By coomassie staining I see basically no induction at all. The protein sequence has quite a few arginines and lysines (so fairly basic) and perhaps this is a problem, although solubility may not be an issue! If anyone has any experience in expressing a large protein in E. coli I would like to hear how you got the thing to be expressed efficiently. I am currently trying 0.1 mM IPTG induction overnight at 37 and also I am trying the host strain ad494(DE3) and ad494(DE3)-lys. Any and all tips/protocols are much appreciated. Cheers, Graham Dellaire -- ******************** Graham Dellaire, Ph.D. MRC Human Genetics Unit Western General Hospital Crewe Road Edinburgh, UK EH4 2XU Fax: +44 (0)131 343 2620 Phone: +44 (0)131 332 2471 ********************